Abstract: An object of the present invention is to identify a novel single nucleotide polymorphism (SNP) associated with the onset and the advancement of inflammatory diseases such as myocardial infarction. The present invention provides a method for judging an inflammatory disease which comprises detecting at least 1 type of genetic polymorphism existing in at least one gene selected from the group consisting of the LBP-32 gene, the TSBP gene, and the WAP gene.

Abstract: An object of the present invention is to identify a novel single nucleotide polymorphism (SNP) associated with the onset and the advancement of inflammatory diseases such as myocardial infarction. The present invention provides a method for judging an inflammatory disease which comprises detecting at least 1 type of genetic polymorphism existing in at least one gene selected from the group consisting of the LBP-32 gene, the TSBP gene, and the WAP gene.

Abstract: It is intended to easily dispense a minute amount of nonvolatile liquid. In a preferred embodiment, in dispensing of mineral oil (nonvolatile liquid), dispensing is conducted in the condition that the amount of air contained in a tip (70) is small by aspirating a larger amount of mineral oil (40) than a single dispensing amount in the tip (70) of a nozzle (28).

Abstract: A single nucleotide polymorphism occurring on a leptin receptor gene is analyzed and an inflammatory disease is examined on the basis of the analytical data. Further, a substance capable of changing the interaction between the leptin receptor and galectin-2 is selected to thereby screen a remedy for an inflammatory disease.

Abstract: An object of the present invention is to identify a novel single nucleotide polymorphism (SNP) associated with the onset and the advancement of inflammatory diseases such as myocardial infarction. The present invention provides a method for judging an inflammatory disease which comprises detecting at least 1 type of genetic polymorphism existing in at least one gene selected from the group consisting of the LBP-32 gene, the TSBP gene, and the WAP gene.

Abstract: A single nucleotide polymorphism occurring on a leptin receptor gene is analyzed and an inflammatory disease is examined on the basis of the analytical data. Further, a substance capable of changing the interaction between the leptin receptor and galectin-2 is selected to thereby screen a remedy for an inflammatory disease.

Abstract: It is intended to easily dispense a minute amount of nonvolatile liquid. In a preferred embodiment, in dispensing of mineral oil (nonvolatile liquid), dispensing is conducted in the condition that the amount of air contained in a tip (70) is small by aspirating a larger amount of mineral oil (40) than a single dispensing amount in the tip (70) of a nozzle (28).

Abstract: It is intended to facilitate dispensing of a minute amount of a nonvolatile liquid. In a preferred embodiment, in dispensing of mineral oil (40) onto a reaction solution (170) that is previously dispensed to a probe arrangement part (18), a liquid droplet (40a) of the mineral oil (40) is formed on a tip end of a tip (70) of the nozzle, and the liquid droplet (40a) is transferred into the reaction well while it is in contact with the inner wall face of the reaction well or the surface of the reaction solution (170) previously dispensed to the reaction well.

Abstract: The object of the present invention is to determine inflammatory diseases such as myocardial infarction by identifying single nucleotide polymorphisms (SNPs) associated with myocardial infarction and utilizing these SNPs. The present invention provides a method for determining an inflammatory disease, which comprises detecting at least one gene polymorphism existing in at least one gene selected from the group consisting of a lymphotoxin-? (LT-?) gene, an I Kappa B-like (IKBL) gene, and a BAT1 gene; an oligonucleotide used in said method; a kit for diagnosing an inflammatory disease which comprises said oligonucleotide; and use thereof. The present invention further provides a method for treating an inflammatory disease; and a method for screening for a therapeutic agent for an inflammatory disease.

Abstract: A reaction vessel suitable for automating of various measurements is provided. In a preferred embodiment, on the same side of plate-like substrate (10), sample injection part (12), typing reagent reservoir part (14) and mineral oil reservoir part (16) are provided as concaves, and further, multiple probe arrangement parts (18) are provided. The typing reagent reservoir part (14) and mineral oil reservoir part (16) are sealed with film (20). The surface of the substrate (10) is covered with detachable sealing material (22) with a size capable of covering the sample injection part (12), typing reagent reservoir part (14), mineral oil reservoir part (16) and multiple probe arrangement parts (18) in such a manner that the film (20) is covered by the sealing material (22). Liquid transfer is carried out through nozzles.

Abstract: In a preferred embodiment, a gene polymorphism diagnosing reaction vessel at least having a plurality of probe arrangement parts each individually holding a probe that emits fluorescence in accordance with each of a plurality of polymorphic sites is used. A controller (118) determines presence or absence of a gene polymorphism based on a fluorescence intensity value (a time-course gradient) per unit time of a fluorescence detection value obtained from a fluorescence detector (64).

Abstract: The object of the invention is to carry out typing for multiple SNP sites automatically from the stage of sample preparation. A mixture of sample (2) and PCR reaction solution (4) is subjected to PCR reaction according to a given temperature cycle. After the completion of PCR reaction, invader reagent (6) is added thereto. Subsequently, the reaction mixture having the invader reagent (6) added thereto is added to probe fixing part (8) of typing reaction zone to thereby effect reaction therebetween. Invader probes capable of emitting fluorescence in respective correspondence to multiple SNP sites are separately held on individual sites of the probe fixing part (8), so that the reaction mixture reacts with the invader probes and when SNPs corresponding to the invader probes exist, fluorescence is emitted.

Abstract: An object of the present invention is to identify a novel single nucleotide polymorphism (SNP) associated with the onset and development of inflammatory diseases such as myocardial infarction. The present invention provides a method for judging inflammatory diseases which comprises detecting at least one gene polymorphism in the galectin-2 gene.

Abstract: The object of the present invention is to determine inflammatory diseases such as myocardial infarction by identifying single nucleotide polymorphisms (SNPs) associated with myocardial infarction and utilizing these SNPs. The present invention provides a method for determining an inflammatory disease, which comprises detecting at least one gene polymorphism existing in at least one gene selected from the group consisting of a lymphotoxin-? (LT-?) gene, an I Kappa B-like (IKBL) gene, and a BAT1 gene; an oligonucleotide used in said method; a kit for diagnosing an inflammatory disease which comprises said oligonucleotide; and use thereof. The present invention further provides a method for treating an inflammatory disease; and a method for screening for a therapeutic agent for an inflammatory disease.

Abstract: The present invention enables typing nearly hundreds of thousands of SNP sites using remarkably a small amount of genomic DNA. That is, the method for SNP typing of the present invention comprises the steps of simultaneously amplifying a plurality of nucleotide sequences comprising at least one or more sites of single nucleotide polymorphism (SNP) using genomic DNA and a plurality of primer pairs; and typing for distinguishing nucleotides of SNP sites contained in the plurality of nucleotide sequences amplified by the above amplification step, using the amplified nucleotide sequences.