Abstract: The present disclosure provides a kit and method for constructing a long-acting depression animal model. The present disclosure adopts Bacille Calmette-Guerin (BCG) (or Mycobacterium bovis (M bovis)) and low-dose pertussis toxin (PTX) for combined induction, which greatly reduces modeling cost. A mouse model constructed by the method of the present disclosure can clearly distinguish a time interval of pathological behaviors from a time interval of depression-like behaviors and has prominent face validity and predictive validity. The method of the present disclosure is simple and convenient and does not require a large number of tedious daily operations as the depression model construction by chronic unpredictable stress, chronic restraint stress, social defeat, mother-child separation, or the like. The mouse model of the present disclosure can provide a valuable long-term intervention window for research on antidepressant therapy.

Abstract: A message processing method includes: displaying first message content and second message content; moving the first message content to the second message content in response to a continuous action operation for the first message content; and displaying merged message content in response to an end of the continuous action operation and that the first message content is moved to an area where the second message content is located, the merged message content including the first message content and the second message content.

Abstract: The present disclosure provides a successive gas path fault diagnosis method with high precision for gas turbine engines and falls within the technical field of fault diagnosis for gas turbine engines, including the following steps: establishing an engine nonlinear component-level model; capturing dynamic effects of an engine transient maneuver; outputting an estimated value of an engine observation parameter by the engine nonlinear component-level model; acquiring a measurement of the engine observation parameter through sensors; and iteratively updating a degradation factor through a solver. The present disclosure captures the dynamic effects of the transient maneuver at consecutive moments through time-series gas path measurement parameters, thereby realizing successive and high-precision diagnosis for health conditions of the gas turbine engines. This technology can provide a new successive and high-precision diagnosis method for the gas turbine engines under steady-state and transient conditions.

Abstract: The invention provides herpes virus nucleic acid vectors for expressing shRNAs in mammalian cells and thereby silencing target genes. The vectors include (a) a herpes virus packaging signal sequence; (b) a herpes virus origin of replication; (c) a segment expressing a light-emitting marker; and (d) a transfer RNA promoter upstream of a restriction endonuclease recognition sequence. A segment encoding an shRNA can be cloned into the restriction endonuclease recognition sequence. Thus, the invention also provides vectors containing: (a) a herpes virus packaging signal sequence; (b) a herpes virus origin of replication; (c) a segment expressing a light-emitting marker; and (d) a transfer RNA promoter linked to (e) a segment encoding a short hairpin RNA (shRNA) that is adapted to degrade in vivo to a small interference RNA (siRNA) that is complementary to a segment of a target gene.