Patents by Inventor Yuanfa Liu

Yuanfa Liu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11946088
    Abstract: The disclosure discloses a method for processing oil crops with rhodotorula, and belongs to the technical field of fermentation. The method includes the step of inoculating the rhodotorula (such as Rhodotorula mucilaginosa, Sporidiobolus salmonicolor and Rhodotorula glutinis) that can produce carotenoid into a fermentation medium that contains oil-rich oil crops for solid state fermentation to obtain oil and oil crop meal rich in carotenoid. The carotenoid as a fermentative metabolite of the rhodotorula has bioactivities of resisting oxidation, preventing vascular sclerosis, enhancing immunity and preventing cancers. Contents of carotenoid in the oil and oil crop meal acquired by the method can be up to 9.071 ?g/g and 8.062 ?g/g correspondingly.
    Type: Grant
    Filed: June 18, 2021
    Date of Patent: April 2, 2024
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Yuanfa Liu, Zhongyang Ding, Yunzhi Jiang, Liting Zhao, Mengmeng Xu, Qi Zhang, Zhenghua Gu, Guiyang Shi
  • Publication number: 20230416302
    Abstract: Disclosed are hemp seed protein Pickering particles as well as a preparation method and application thereof, belonging to the field of multi-component restructured foods. The preparation of Pickering emulsion based on hemp seed protein Pickering nanoparticles includes: preparing hemp seed protein Pickering particles by using an anti-solvent precipitation method under the condition of taking hemp seed protein as a raw material, formic acid and water as solvents; then, adding the prepared hemp seed protein Pickering particles into water together with maltodextrin and corn syrup solids for fully dissolving to form a homogeneous suspension; and adding vegetable oil into the suspension and mixing well to obtain the Pickering emulsion. The Pickering emulsion is high in stability, and powdered oil constructed by using same has the advantages of being environmentally-friendly, cost-saving, and easy to achieve high stability and high unsaturation, thus having wide application value in food and medicine fields.
    Type: Application
    Filed: September 8, 2023
    Publication date: December 28, 2023
    Inventors: Yuanfa Liu, Xiuhang Chai, Yanwen Sun, Wanjun Han
  • Publication number: 20230406891
    Abstract: The present disclosure discloses the dual-functional bean-derived polypeptides and a preparation method therefor, and belongs to the technical field of functional polypeptides. The method includes extracting a bean source protein isolate from beans by an alkali-solution and acid-isolation method first, then subjecting the bean source protein isolate to infrared heat treatment, and finally performing enzymolysis with a protease to obtain the bean-derived polypeptides. The bean-derived polypeptides have dual functions of an antihypertensive activity and an antioxidant activity at the same time.
    Type: Application
    Filed: August 29, 2023
    Publication date: December 21, 2023
    Inventors: Yuanfa Liu, Zhaojun Zheng
  • Patent number: 11845908
    Abstract: The disclosure discloses a method for preparing functional grease through segmented solid-state fermentation of mixed fungi, and belongs to the field of edible oil processing. According to the disclosure, soybeans are used as a main fermentation substrate, for the requirements for different functional nutrition, the soybeans are matched with other oil seeds, fungus segmented solid-state fermentation is used and combined with a supercritical extraction fractionation technology, so that the purposes of improving the aftertaste of soybean oils and generating different flavors are achieved, and the nutritional value of the grease is improved. The fungus segmented solid-state fermentation of the disclosure can improve the oil yield, improve the fatty acid composition of the grease, and increase the content of main flavor substances such as pyrazines, alcohols and phenols, and the sensory functions such as flavor and color of the grease more meet the requirements of consumers for innovative products.
    Type: Grant
    Filed: June 30, 2020
    Date of Patent: December 19, 2023
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Yuanfa Liu, Jinwei Li, Yinghong Zhai
  • Publication number: 20230271109
    Abstract: The present disclosure discloses a method for accurately separating and identifying an oxidized triglyceride in frying oil, and belongs to the technical field of detection. In the present disclosure, the structure of the oxidized triglyceride (ox-TG) in the frying oil is identified by mass spectrometry first. It is found that after frying is conducted for 24 h, the ox-TG mainly includes epoxy ox-TG, hydroxyl ox-TG, and aldehyde ox-TG. Thus, the three types of ox-TG are selected as a template molecule to synthesize a surface molecularly imprinted polymer (SMIPs). Then, a polymer completely matched with the ox-TG template molecule in action site and spatial configuration is synthesized, and the specific ox-TG can be separated by using the SMIPs. According to the present disclosure, OXTG-SMIPs prepared by a molecular imprinting technology has good specificity, stability, and affinity, and accurate separation of the ox-TG in the frying oil can be achieved.
    Type: Application
    Filed: April 17, 2023
    Publication date: August 31, 2023
    Inventors: Yuanfa Liu, Xinyu Cao, Yongjiang Xu, Liyang Yuan, Yanpeng Du, Zhaojun Zheng
  • Patent number: 11733230
    Abstract: The disclosure discloses a method for quickly and accurately analyzing polyphenol content in rapeseed oil, and belongs to the field of analysis of natural compounds. The separation method of the disclosure uses acetonitrile-water as an extractant to extract polyphenols from the rapeseed oil, and cooperates with a C18 adsorbent for purification, and then performs separation and purification. Compared with the traditional liquid-liquid extraction and solid-phase extraction, the method has an average recovery rate of polyphenols in the rapeseed oil of 81.31% to 102.95%, and RSDs of 0.86% to 8.03%, and has higher accuracy and precision. The method of the disclosure not only uses less organic reagents and causes less environmental pollution, but also reduces matrix interference and improves purification efficiency through optimization of the adsorbent.
    Type: Grant
    Filed: December 31, 2020
    Date of Patent: August 22, 2023
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Yuanfa Liu, Junge Song, Yongjiang Xu, Zhaojun Zheng, Chen Cao, Yinghong Zhai
  • Publication number: 20230225362
    Abstract: The present disclosure discloses an aquatic-plant protein combined restructured meat and a preparation method thereof, and belongs to the technical field of processing of foods. According to the present disclosure, a protein combined restructuring processing technology can be summarized as including pretreatment of an aquatic-plant protein, combined restructuring, high-temperature qualitative treatment, and integrated forming. The whole process is green, safe, and environmentally friendly. The protein combined restructured meat prepared contains two proteins including an animal protein and a plant protein, and has a protein content of greater than 20%. While the demands for nutritional dietary proteins are met, the chewability is greater than 1,500 g·mm, and the viscoelasticity is excellent. The meat can be processed in various shapes according to needs, directly heated for eating, or processed into a prepared food, a semi-finished product and the like, so that the product has a large application space.
    Type: Application
    Filed: March 23, 2023
    Publication date: July 20, 2023
    Inventors: Yuanfa LIU, Zhaojun ZHENG, Liyang YUAN
  • Publication number: 20230092674
    Abstract: The present disclosure discloses application of a microbiota-derived plasmalogens to treatment of colon cancer, and belongs to the technical field of biomedicine and microorganisms. The microbiota-derived plasmalogens provided in the present disclosure is extracted from an anaerobic microorganism including any one or more of Lactobacillaceae, Bifidobacterium, Clostridium butyricum, and Peptostreptococcus Kluyver and van Niel after fermentation. The microbiota-derived plasmalogens can reduce expressions of colon cancer-associated cytokines at the molecular level, inhibit the proliferation of colon cancer cells, and reduce the number and size of colon adenomas in patients with colon cancer. The microbiota-derived plasmalogens can be used as an effective nutrition strategy for preventing and treating the colon cancer, and a theoretical support is provided for efficient utilization of the enteric microbiota-derived plasmalogens.
    Type: Application
    Filed: November 21, 2022
    Publication date: March 23, 2023
    Inventors: Yuanfa LIU, Yanjun LIU, Yongjiang XU
  • Patent number: 11319569
    Abstract: The disclosure provides an active peptide with an anti-lipid oxidation function and a preparation method and application thereof and belongs to the technical field of plant-derived biologically active peptides. In the disclosure, oil processing by-products, namely oil crops after oil extraction, are used as the raw materials, and the raw materials are subjected to the steps of protein extraction, infrared pretreatment, proteolysis, freeze-drying, lipophilic part extraction, vacuum concentration and drying to prepare an anti-lipid oxidation peptide having the functional characteristics of scavenging DPPH free radicals, chelating metal ions, inhibiting lipid peroxidation, prolonging vegetable oil oxidation induction time, improving emulsion stability and the like.
    Type: Grant
    Filed: December 29, 2020
    Date of Patent: May 3, 2022
    Assignee: Jiangnan University
    Inventors: Yuanfa Liu, Zhaojun Zheng, Jiaxin Li, Yongjiang Xu, Yin Chen
  • Patent number: 11319565
    Abstract: The disclosure discloses a method for preparing a lipoamino acid compound based on deep eutectic solvents, and belongs to the field of organic synthesis. The preparation method of the disclosure uses a fatty acid and an amino acid as substrates, uses a deep eutectic solvent as a medium, and directly and efficiently prepares a single-chain lipoamino acid surfactant under the catalysis of lipase CALB. Alternatively, Amano lipase M is used as a catalyst to efficiently catalyze the reaction of an amino acid containing a plurality of amino groups with a fatty acid to prepare a Gemini amino acid. The method of the disclosure does not need to undergo protection and deprotection of the amino acid, and does not involve commonly used fatty acid chlorides. Compared with the traditional chemical synthesis methods, the disclosure has the advantages of greenness, safety, high selectivity, high yield, etc., and has very important potential application value.
    Type: Grant
    Filed: December 31, 2020
    Date of Patent: May 3, 2022
    Assignee: Jiangnan University
    Inventors: Yuanfa Liu, Binbin Nian, Chen Cao, Zhaojun Zheng, Yongjiang Xu, Yinghong Zhai
  • Publication number: 20220046964
    Abstract: The present disclosure discloses a method for modification of mung bean protein and preparation of simulated egg pulp based on the modified protein. A commercial mung bean protein isolate is used as a raw material in the present disclosure, then subjected to pH shift modification, ultrasonic treatment and protein-glutaminase treatment for compound modification to prepare a high-functionality modified mung bean protein isolate; and the modified mung bean protein isolate is used as a raw material, using high-speed emulsification and high-pressure homogenization are conducted to obtain a high-stability mung bean protein-based simulated egg pulp. In the present disclosure, the mung bean protein isolate prepared by compound modification has good solubility, emulsifying property and gelling property. The mung bean protein-based simulated egg pulp is in the form of a stable emulsion and has good fluidity and moderate gelling property, and uniformity and stability can still be maintained for a long time.
    Type: Application
    Filed: November 1, 2021
    Publication date: February 17, 2022
    Inventors: Jiang JIANG, Ying WANG, Shucheng ZHANG, Youling XIONG, Yuanfa LIU
  • Publication number: 20210322497
    Abstract: The disclosure discloses a method of fermenting soybeans or other oil crops by edible and medicinal fungi, and belongs to the technical field of fermentation. The method involves solid-state fermentation by inoculating edible and medicinal fungi such as i Ganoderma lucidum, Tremella aurantialba, Hericium erinaceus and Cordyceps sinensis that can produce bioactive substances such as active polysaccharides, flavonoids and triterpenoids in a fermentation substrate containing oily crops such as extruded soybeans, peanuts and rapeseeds, thereby obtaining oil and oil crop meal rich in the bioactive substances. In the oil obtained by the method, the content of flavonoids is as high as 0.302 mg/g, and the content of triterpenoids is as high as 65.663 mg/g. In the oil crop meal, the content of active polysaccharides is as high as 60.651 mg/g, the content of flavonoids is as high as 1.599 mg/g, and the content of triterpenoids is as high as 14.225 mg/g.
    Type: Application
    Filed: June 19, 2021
    Publication date: October 21, 2021
    Inventors: Zhongyang DING, Yuanfa LIU, Yunzhi JIANG, Mengmeng XU, Liting ZHAO, Chen CAO, Qiong WANG, Zhenghua GU, Guiyang SHI
  • Publication number: 20210310035
    Abstract: The disclosure discloses a method for processing oil crops with Rhodotorula, and belongs to the technical field of fermentation. The method includes the step of inoculating the Rhodotorula (such as Rhodotorula mucilaginosa, Sporidiobolus salmonicolor and Rhodotorula glutinis) that can produce carotenoid into a fermentation medium that contains oil-rich oil crops for solid state fermentation to obtain oil and oil crop meal rich in carotenoid. The carotenoid as a fermentative metabolite of the Rhodotorula has bioactivities of resisting oxidation, preventing vascular sclerosis, enhancing immunity and preventing cancers. Contents of carotenoid in the oil and oil crop meal acquired by the method can be up to 9.071 ?g/g and 8.062 ?g/g correspondingly.
    Type: Application
    Filed: June 18, 2021
    Publication date: October 7, 2021
    Inventors: Yuanfa LIU, Zhongyang DING, Yunzhi JIANG, Liting ZHAO, Mengmeng XU, Qi ZHANG, Zhenghua GU, Guiyang SHI
  • Patent number: 11064719
    Abstract: The disclosure discloses a production method of marbled beef, and belongs to the field of food processing. The processing steps of the method are: freezing, thawing of beef, preparation of a gel emulsion, injection, wrap shaping, quick freezing, cutting and packaging, and hot dipping. The preparation method of the process of the disclosure can realize high-efficiency and standardized industrial production. By injecting beef with the formulated gel emulsion, the fat content and high-quality protein content of the beef can be increased, thereby improving the sensory properties and flavor of the beef, and producing high-quality beef. The gel emulsion for preparing marbled beef of the disclosure is liquid and is low in viscosity when the temperature is higher than 40° C., and therefore the gel emulsion is convenient for injection into beef, and can be uniformly dispersed in beef to form a delicate marble pattern. When the temperature is lower than 20° C., the gel emulsion forms a solid in beef tissue.
    Type: Grant
    Filed: February 28, 2020
    Date of Patent: July 20, 2021
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Yuanfa Liu, Zhanyang Guo, Jinwei Li, Chen Cao, Jiang Jiang
  • Publication number: 20210130862
    Abstract: The disclosure discloses a method for preparing a lipoamino acid compound based on deep eutectic solvents, and belongs to the field of organic synthesis. The preparation method of the disclosure uses a fatty acid and an amino acid as substrates, uses a deep eutectic solvent as a medium, and directly and efficiently prepares a single-chain lipoamino acid surfactant under the catalysis of lipase CALB. Alternatively, Amano lipase M is used as a catalyst to efficiently catalyze the reaction of an amino acid containing a plurality of amino groups with a fatty acid to prepare a Gemini amino acid. The method of the disclosure does not need to undergo protection and deprotection of the amino acid, and does not involve commonly used fatty acid chlorides. Compared with the traditional chemical synthesis methods, the disclosure has the advantages of greenness, safety, high selectivity, high yield, etc., and has very important potential application value.
    Type: Application
    Filed: December 31, 2020
    Publication date: May 6, 2021
    Inventors: Yuanfa LIU, Binbin NIAN, Chen CAO, Zhaojun ZHENG, Yongjiang XU, Yinghong ZHAI
  • Publication number: 20210123897
    Abstract: The disclosure discloses a method for quickly and accurately analyzing polyphenol content in rapeseed oil, and belongs to the field of analysis of natural compounds. The separation method of the disclosure uses acetonitrile-water as an extractant to extract polyphenols from the rapeseed oil, and cooperates with a C18 adsorbent for purification, and then performs separation and purification. Compared with the traditional liquid-liquid extraction and solid-phase extraction, the method has an average recovery rate of polyphenols in the rapeseed oil of 81.31% to 102.95%, and RSDs of 0.86% to 8.03%, and has higher accuracy and precision. The method of the disclosure not only uses less organic reagents and causes less environmental pollution, but also reduces matrix interference and improves purification efficiency through optimization of the adsorbent.
    Type: Application
    Filed: December 31, 2020
    Publication date: April 29, 2021
    Inventors: Yuanfa LIU, Junge SONG, Yongjiang XU, Zhaojun ZHENG, Chen CAO, Yinghong ZHAI
  • Publication number: 20210115488
    Abstract: The disclosure provides an active peptide with an anti-lipid oxidation function and a preparation method and application thereof and belongs to the technical field of plant-derived biologically active peptides. In the disclosure, oil processing by-products, namely oil crops after oil extraction, are used as the raw materials, and the raw materials are subjected to the steps of protein extraction, infrared pretreatment, proteolysis, freeze-drying, lipophilic part extraction, vacuum concentration and drying and the like to prepare an anti-lipid oxidation peptide having the functional characteristics of scavenging DPPH free radicals, chelating metal ions, inhibiting lipid peroxidation, prolonging vegetable oil oxidation induction time, improving emulsion stability and the like.
    Type: Application
    Filed: December 29, 2020
    Publication date: April 22, 2021
    Inventors: Yuanfa LIU, Zhaojun ZHENG, Jiaxin LI, Yongjiang XU, Yin CHEN
  • Publication number: 20200332220
    Abstract: The disclosure discloses a method for preparing functional grease through segmented solid-state fermentation of mixed fungi, and belongs to the field of edible oil processing. According to the disclosure, soybeans are used as a main fermentation substrate, for the requirements for different functional nutrition, the soybeans are matched with other oil seeds, fungus segmented solid-state fermentation is used and combined with a supercritical extraction fractionation technology, so that the purposes of improving the aftertaste of soybean oils and generating different flavors are achieved, and the nutritional value of the grease is improved. The fungus segmented solid-state fermentation of the disclosure can improve the oil yield, improve the fatty acid composition of the grease, and increase the content of main flavor substances such as pyrazines, alcohols and phenols, and the sensory functions such as flavor and color of the grease more meet the requirements of consumers for innovative products.
    Type: Application
    Filed: June 30, 2020
    Publication date: October 22, 2020
    Inventors: Yuanfa LIU, Jinwei LI, Yinghong ZHAI
  • Publication number: 20200196642
    Abstract: The disclosure discloses a production method of marbled beef, and belongs to the field of food processing. The processing steps of the method are: freezing, thawing of beef, preparation of a gel emulsion, injection, wrap shaping, quick freezing, cutting and packaging, and hot dipping. The preparation method of the process of the disclosure can realize high-efficiency and standardized industrial production. By injecting beef with the formulated gel emulsion, the fat content and high-quality protein content of the beef can be increased, thereby improving the sensory properties and flavor of the beef, and producing high-quality beef. The gel emulsion for preparing marbled beef of the disclosure is liquid and is low in viscosity when the temperature is higher than 40° C., and therefore the gel emulsion is convenient for injection into beef, and can be uniformly dispersed in beef to form a delicate marble pattern. When the temperature is lower than 20° C., the gel emulsion forms a solid in beef tissue.
    Type: Application
    Filed: February 28, 2020
    Publication date: June 25, 2020
    Inventors: Yuanfa LIU, Zhanyang GUO, Jinwei LI, Chen CAO, Jiang JIANG
  • Patent number: 10640728
    Abstract: The invention discloses a method for preparing rapeseed oil by a semi-solid aqueous enzymatic process, belonging to the field of functional foods and health care products. The present invention first prepares a semi-solid rapeseed paste with 3.5-4.5% moisture content, which is hydrolyzed by a mixture of pectinase, cellulase and alkaline protease to extract rapeseed oil. The resulting rapeseed oil contains high levels of active ingredients including totaxin, sterol, phenols and beta-carotene. The rapeseed oil of the invention can be added into animal feeds, which helps to reduce animal blood lipid levels and body weight. It can significantly reduce the contents of total triglyceride, total cholesterol and LDC-C in the blood, and, at the same time, increases the level of HDL-C in the blood. In addition, the rapeseed oil prepared by the present invention can relieve hepatic steatosis in hyperlipemia rats.
    Type: Grant
    Filed: May 15, 2018
    Date of Patent: May 5, 2020
    Assignee: Jiangnan University
    Inventors: Yuanfa Liu, Peirang Cao, Jinwei Li, Liang Zhang