Abstract: Disclosed is a method for reducing pressure and increasing injection by continuous operation system of biological acid acidification and nano coating, specifically including composition and preparation method of biological acidizing agent and nano coating agent. The method for reducing pressure and increasing injection by continuous operation system of biological acid acidification and nano coating of the present disclosure is able to effectively dredge the stratum water flow channel, reduce the water flow resistance, and achieve the effect of high-efficiency pressure reduction and injection increase of the water injection well.

Abstract: The present disclosure relates to the technical field of application of bioengineering technology in microbial oil recovery, and discloses a Brevibacillus agri strain and preparation thereof and a method for preparing surfactant, and use thereof. The Brevibacillus agri strain is deposited in the China General Microbiological Culture Collection Center under the accession number CGMCC No. 9983. The Brevibacillus agri and its preparation may effectively enhance the crude oil recovery; the method for preparing the surfactant allow the lipopeptide biosurfactant to have good physical properties, effectively reduce the surface tension, and have good emulsifying performance for petroleum, various hydrocarbons and lipids.

Abstract: A biochemical viscosity reducer for heavy oil and a preparation method thereof. The viscosity reducer includes: Brevibacillus borstelensis-fermented mixed lipopeptide solution: 30 to 60 parts; compound biological enzyme: 15 to 30 parts; plant-based nonionic surfactant: 10 to 20 parts; antibacterial agent: 1 to 5 parts; stabilizer: 1 to 5 parts; and alcohol solvent: 10 to 20 parts; where, the above components are measured by mass. The preparation method includes: step 1: adding 30 to 60 parts of a Brevibacillus borstelensis-fermented mixed lipopeptide solution, 15 to 30 parts of a compound biological enzyme, 10 to 20 parts of a plant-based nonionic surfactant, and 1 to 5 parts of a stabilizer to a reactor; and step 2: adding 1 to 5 parts of an antibacterial agent and an alcohol solvent to the reactor, and stirring a resulting mixture for 60 min to 120 min.

Abstract: An iron-reducing Tessaracoccus oleiagri strain DH10 and applications of the iron-reducing Tessaracoccus oleiagri strain DH10 are provided. The Tessaracoccus oleiagri strain DH10 had been preserved in China center for type culture collection on Apr. 19, 2021, with a preservation number of CCTCC No: M 2021404. The iron-reducing Tessaracoccus oleiagri strain DH10 can efficiently reduce Fe(III) is separated and screened from oil reservoir environment. SEM analysis shows that it can effectively decompose lean iron montmorillonite minerals and inhibit expansion of clay. Moreover, core experiments show that biological agent of the Tessaracoccus oleiagri strain DH10 can reduce water sensitivity of reservoir core and water injection pressure, and can be applied to crude oil recovery to effectively improve crude oil recovery factor.

Abstract: The present disclosure relates to the technical field of application of bioengineering technology in microbial oil recovery, and discloses a Brevibacillus agri strain and preparation thereof and a method for preparing surfactant, and use thereof. The Brevibacillus agri strain is deposited in the China General Microbiological Culture Collection Center under the accession number CGMCC No. 9983. The Brevibacillus agri and its preparation may effectively enhance the crude oil recovery; the method for preparing the surfactant allow the lipopeptide biosurfactant to have good physical properties, effectively reduce the surface tension, and have good emulsifying performance for petroleum, various hydrocarbons and lipids.

Abstract: A biochemical viscosity reducer for heavy oil and a preparation method thereof. The viscosity reducer includes: Brevibacillus borstelensis-fermented mixed lipopeptide solution: 30 to 60 parts; compound biological enzyme: 15 to 30 parts; plant-based nonionic surfactant: 10 to 20 parts; antibacterial agent: 1 to 5 parts; stabilizer: 1 to 5 parts; and alcohol solvent: 10 to 20 parts; where, the above components are measured by mass. The preparation method includes: step 1: adding 30 to 60 parts of a Brevibacillus borstelensis-fermented mixed lipopeptide solution, 15 to 30 parts of a compound biological enzyme, 10 to 20 parts of a plant-based nonionic surfactant, and 1 to 5 parts of a stabilizer to a reactor; and step 2: adding 1 to 5 parts of an antibacterial agent and an alcohol solvent to the reactor, and stirring a resulting mixture for 60 min to 120 min.

Abstract: The invention provides a quick method for detecting the genus of Bacillus in samples from oil reservoirs, which comprise: collecting samples from oil reservoirs; inactivating microbes except for spore-forming Bacillus in the samples through high temperature; collecting spores from the samples; incubating the collected spores in a medium, and stimulating to resurrect Bacillus under in situ temperatures of the oil reservoirs; detecting the resurrected Bacillus in the cultures using molecular biological techniques. The present invention provides an effective method to detect the genus of Bacillus in samples from oil reservoirs, which is conducive to the discovery of the functional Bacillus for microbial enhanced oil recovery (MEOR) and to revealing the ecosystem of Bacillus in oil reservoirs.

Abstract: The invention provides a quick method for detecting the genus of Bacillus in samples from oil reservoirs, which comprise: collecting samples from oil reservoirs; inactivating microbes except for spore-forming Bacillus in the samples through high temperature; collecting spores from the samples; incubating the collected spores in a medium, and stimulating to resurrect Bacillus under in situ temperatures of the oil reservoirs; etecting the resurrected Bacillus in the cultures using molecular biological techniques. The present invention provides an effective method to detect the genus of Bacillus in samples from oil reservoirs, which is conducive to the discovery of the functional Bacillus for microbial enhanced oil recovery (MEOR) and to revealing the ecosystem of Bacillus in oil reservoirs.