Abstract: A method for estimating a purified state includes quantifying a component that is included in a treatment liquid obtained by performing a purification treatment on a liquid including a specific protein and impurities other than the protein. The method for estimating a purified state includes acquiring an estimated value of a concentration of the impurities on the basis of spectral data indicating an intensity of electromagnetic waves, which have been emitted to the treatment liquid and have been subjected to an action of the treatment liquid, for each wave number or wavelength. The concentration of the impurities included in the treatment liquid is equal to or less than 20 mg/mL, and a weight ratio of the impurities to a mixture including the protein and the impurities is equal to or less than 15%.

Abstract: A single-use cell culturing apparatus and a culturing bag are provided, each of which can improve efficiency of stirring a culture solution and further reduce a possibility of leakage of the culture solution. The single-use cell culturing apparatus of the present invention includes a housing that accommodates the culturing bag which is flexible and can seal the culture solution inside thereof. The housing has, on an inner surface thereof, a curved convex-shaped protrusion provided at a portion of a contact face at which the housing is in contact with the culturing bag. The culturing bag of the present invention is accommodated in the housing of the single-use cell culturing apparatus; is flexible; and can seal the culture solution inside thereof. The culturing bag includes a concave portion having a shape corresponding to the curved convex protrusion disposed on a part of the housing.

Abstract: A single-use cell culturing apparatus and a culturing bag are provided, each of which can improve efficiency of stirring a culture solution and further reduce a possibility of leakage of the culture solution. The single-use cell culturing apparatus of the present invention includes a housing that accommodates the culturing bag which is flexible and can seal the culture solution inside thereof. The housing has, on an inner surface thereof, a curved convex-shaped protrusion provided at a portion of a contact face at which the housing is in contact with the culturing bag. The culturing bag of the present invention is accommodated in the housing of the single-use cell culturing apparatus; is flexible; and can seal the culture solution inside thereof. The culturing bag includes a concave portion having a shape corresponding to the curved convex protrusion disposed on a part of the housing.

Abstract: Provided is a purification device for purifying a useful substance (i.e., target substance) without using an acidic eluent. The purification device purifying the target substance comprises a column and a filler packed in the column. The filler contains a carrier, a temperature responsive polyamino acid bonded to the carrier, and a binding substance bonded to the temperature responsive polyamino acid and specifically bonding to the target substance.

Abstract: Provided are a cell separation and collection membrane capable of suppressing non-specific adsorption of cells while specifically adsorbing target cells; a culturing sheet and a culturing apparatus which use the membrane; and a cell separation and collection method. The cell separation and collection membrane included in the culturing sheet comprises: a support; a polymer site fixed to the support and having a changeable structure in accordance with a temperature; a cell adsorbing site bound to the polymer site, exposed relative to an outer surface of the support, and specifically adsorbing a target cell when the cell adsorbing site is brought into contact with a treatment solution containing the target cells; and a hydrophilic site exposed relative to the outer surface and to be brought into contact with the treatment solution.

Abstract: Provided is a purification device for purifying a useful substance (i.e., target substance) without using an acidic eluent. The purification device purifying the target substance comprises a column and a filler packed in the column. The filler contains a carrier, a temperature responsive polyamino acid bonded to the carrier, and a binding substance bonded to the temperature responsive polyamino acid and specifically bonding to the target substance.