Abstract: [Problem] Provision of a method for fractionating lipids using liquefied dimethyl ether as a solvent. [Means for Solving] To provide a method for fractionating a lipid using liquefied dimethyl ether as a solvent. [Solution] A method for fractionating a lipid, which comprises subjecting a microbial biomass to extraction using liquefied dimethyl ether as a solvent and then fractionating the lipid utilizing separation selectivity for the lipid. A method for producing a lipid, which comprises subjecting a microbial biomass to extraction using liquefied dimethyl ether as a solvent, then fractionating a portion of the lipid utilizing separation selectivity for the lipid to modify the fatty acid composition of the remainder of the lipid which remains in the microbial biomass, and then extracting the lipid having a modified fatty acid composition.

Abstract: Docosahexaenoic acid-containing oil containing docosahexaenoic acid in a concentration of 40 wt. % or more of the total weight of fatty acids in the oil, and having an endothermic peak temperature determined by differential scanning calorimetry (DSC) of 15° C. or lower; a biomass including the same; and a method for producing docosahexaenoic acid-containing oil including obtaining a biomass by culturing microorganisms of the genus Aurantiochytrium capable of producing this docosahexaenoic acid-containing oil, recovering the biomass after culture, and extracting the oil from the biomass after recovery.

Abstract: A microbial oil is extracted from stramenopile transformed with a gene associated with synthesis of fatty acids, the gene encoding a fatty acid desaturase. The stramenopile belongs to the class Labyrinthulomycete. The microbial oil satisfies one or more of the following requirements: (a) an amount of arachidonic acid is 7% or less based on a total amount of the fatty acid composition; (b) an amount of DPA is 9% or less based on the total amount of the fatty acid composition; (c) an amount of ETA is 0.04% or more based on the total amount of the fatty acid composition; (d) an amount of EPA is 7% or more based on the total amount of the fatty acid composition; and (e) an amount of DHA is 45% or more based on the total amount of the fatty acid composition.

Abstract: An ozone generation apparatus includes a cylindrical shaped first electrode, a cylindrical shaped second electrode disposed coaxially with the first electrode and disposed in the first electrode, a dielectric disposed between the first electrode and the second electrode. Dry air is supplied between the first electrode and the second electrode as raw material gas. A discharge gap length d formed by the first electrode, the second electrode, and the dielectric is set to be in a range of 0.3 to 0.5 mm. A pd product, which is a product of the discharge gap length d and a gas pressure p of the raw material gas, is in a range of 6 to 16 kPa·cm. And the discharge gap length d and the gas pressure p of the raw material gas are set to satisfy following expression.

Abstract: An ozone generator includes a container, a first metal electrode, a dielectric electrode, a heat pipe, a heat sink, and a power supply unit. The first metal electrode is a cylindrical electrode the axial direction of which is a first direction, and disposed in the container. A cooling medium is supplied to an outer peripheral surface thereof. The dielectric electrode is a cylindrical electrode that is disposed to be opposed to an inner peripheral surface of the first metal electrode, and is coaxial with the first metal electrode. The heat pipe is disposed to be opposed to an inner peripheral surface of the dielectric electrode, and has electrical conductivity. The heat sink is disposed on the outside as an outer space of a space between the first metal electrode and the heat pipe, and connected to the heat pipe.

Abstract: A substrate treatment device includes: a substrate rotation part that horizontally holds and rotates a substrate; a nozzle that supplies a treatment liquid to a surface to be treated of the substrate rotated by the substrate rotation part; an imaging part that captures an image of an imaging area including a plurality of target areas in which a liquid film is formed when the treatment liquid is supplied to the substrate; and a detection part that refers to an imaging result of the imaging part and detects a treatment ending time point of each of the target areas based on a change in luminance value for each of the target areas. Further, the imaging area includes at least an area on a center side of the surface to be treated and an area on an outer circumferential side of the surface to be treated as the target areas.

Abstract: A substrate treatment method is provided, including: holding a substrate on a substrate holding unit; rotating the substrate holding unit to rotate the substrate; raising a cup member surrounding an outer periphery of the substrate holding unit to locate an upper end of the cup member in an upper end position higher than an upper surface of the substrate held on the substrate holding unit; discharging a treatment liquid from a discharge port of a nozzle located lower than the upper end position to an end portion of the upper surface of the substrate held on the substrate holding unit; an imaging process, acquiring captured images of an imaging region by a camera, wherein the imaging region contains the treatment liquid discharged from the discharge port and is seen from an imaging position above the substrate; and determining a discharge state of the treatment liquid based on the captured images.

Abstract: An ozone generator includes a first end plate, a second end plate located opposite the first end plate, a metallic electrode held at both ends by the first and second end plates, a dielectric located inside the metallic electrode with a discharge gap, and including an open end on a first end plate side and a closed end on a second end plate side, a conductive film located on an inner surface of the dielectric, and a high voltage feeding terminal electrically coupled to the conductive film. The conductive film and the high voltage feeding terminal are at least partially in a same position as the first end plate in axial direction of the dielectric. An end of the conductive film and an end of the high voltage feeding terminal on a dielectric opening side extend further toward the opening of the dielectric than the first end plate in the axial direction.

Abstract: In equipment that executes a drying process of forming a liquid membrane on a top surface of a substrate W which is held horizontally and gradually enlarging a dry area from which the liquid membrane has been removed, quality of the drying process is determined. Specifically, first, the top surface of the substrate is repeatedly imaged by an imaging unit during execution of the drying process. Then, it is determined whether the dry area is in a normal state based on a plurality of captured images acquired by the imaging. Accordingly, it is possible to quantitatively determine whether a dry area is in a normal state based on a plurality of captured images.

Abstract: In equipment that supplies a processing liquid on a top surface of a substrate while holding the substrate horizontally in a chamber a generation status of fumes is determined. Specifically, an image of a predetermined imaging area in the chamber is captured. Then, the generation status of fumes in the chamber is determined based on luminance values of the captured image acquired by the capturing of an image. Accordingly, it is possible to quantitatively determine whether a generation status of fumes in a chamber is normal.

Abstract: A microbial oil is obtained from Labyrinthulomycetes in which a gene for fatty acid biosynthesis has been disrupted or an expression of the gene has been inhibited to highly accumulate the fatty acid. The microbial oil typically contains: (a) 1.5% or more of arachidonic acid (AA) based on a total amount of fatty acid; (b) 0.2% or more of dihomo-?-linolenic acid (DGLA) based on the total amount of fatty acid; (c) 0.04% or more of eicosatetraenoic acid (ETA) based on the total amount of fatty acid; (d) 3.8% or more of eicosapentaenoic acid (EPA) based on the total amount of fatty acid; (e) 13.7% or less of n-6 docosapentaenoic acid (n-6DPA) based on the total amount of fatty acid; and (f) 43.9% or less of docosahexaenoic acid (DHA) based on the total amount of fatty acid.

Abstract: A substrate treatment device includes: a substrate rotation part that horizontally holds and rotates a substrate; a nozzle that supplies a treatment liquid to a surface to be treated of the substrate rotated by the substrate rotation part; an imaging part that captures an image of an imaging area including a plurality of target areas in which a liquid film is formed when the treatment liquid is supplied to the substrate; and a detection part that refers to an imaging result of the imaging part and detects a treatment ending time point of each of the target areas based on a change in luminance value for each of the target areas. Further, the imaging area includes at least an area on a center side of the surface to be treated and an area on an outer circumferential side of the surface to be treated as the target areas.

Abstract: A Labyrinthulid microorganism capable of producing a PUFA only through the elongase-desaturase pathway. A Labyrinthulid microorganism which has no ability of producing a PUFA through the endogenous PUFA-PKS pathway or has the ability at an extremely weak level and which has an ability of producing a PUFA through the endogenous elongase-desaturase pathway. The Labyrinthulid microorganism is a microorganism belonging to the genus Parietichytrium or Parietichytrium.

Abstract: A microbial oil is extracted from stramenopile transformed with a gene associated with synthesis of fatty acids, the gene encoding a fatty acid desaturase. The stramenopile belongs to the class Labyrinthulomycete. The microbial oil satisfies one or more of the following requirements: (a) an amount of arachidonic acid is 7% or less based on a total amount of the fatty acid composition; (b) an amount of DPA is 9% or less based on the total amount of the fatty acid composition; (c) an amount of ETA is 0.04% or more based on the total amount of the fatty acid composition; (d) an amount of EPA is 7% or more based on the total amount of the fatty acid composition; and (e) an amount of DHA is 45% or more based on the total amount of the fatty acid composition.

Abstract: Docosahexaenoic acid-containing oil containing docosahexaenoic acid in a concentration of 40 wt. % or more of the total weight of fatty acids in the oil, and having an endothermic peak temperature determined by differential scanning calorimetry (DSC) of 15° C. or lower; a biomass including the same; and a method for producing docosahexaenoic acid-containing oil including obtaining a biomass by culturing microorganisms of the genus Aurantiochytrium capable of producing this docosahexaenoic acid-containing oil, recovering the biomass after culture, and extracting the oil from the biomass after recovery.

Abstract: Whether or not a liquid is discharged from a nozzle is determined from an image. An index value larger in a discharge state than in a non-discharge state is calculated from an evaluation area including a path of the liquid. The discharge state or the non-discharge state is determined according to a relationship between the index value and a reference value. The reference value is determined according to a histogram of index values obtained from a plurality of images collected in advance. When an evaluation value indicating a peak position or the sharpness of a peak is smaller than a predetermined threshold value in the histogram, the collected images are assumed to show the non-discharge state. When the evaluation value is larger than the threshold value, the collected images are assumed to show the discharge state. Thus, the reference value is set for the discharge state and the non-discharge state.

Abstract: A position detection apparatus detects the positions of movable parts 33, 43, 53 which are movable within processing space SP inside a chamber 90. An imaging device 72 shoots reference sections 61-64, which are disposed inside the chamber 90 and the positions of which are known, and the movable parts and accordingly obtains an original image. An image processor 86 executes image processing which is for detecting the movable parts and the reference sections from the original image. An image detector 86 detects the positions of the movable parts within the processing space in accordance with position information which is indicative of the positions of the movable parts and the reference sections within the original image. It is possible to accurately detect the positions of the movable parts while suppressing the influence exerted by deviation of the imaging device.

Abstract: Whether or not a liquid is discharged from a nozzle is determined from an image. An index value larger in a discharge state than in a non-discharge state is calculated from an evaluation area including a path of the liquid. The discharge state or the non-discharge state is determined according to a relationship between the index value and a reference value. The reference value is determined according to a histogram of index values obtained from a plurality of images collected in advance. When an evaluation value indicating a peak position or the sharpness of a peak is smaller than a predetermined threshold value in the histogram, the collected images are assumed to show the non-discharge state. When the evaluation value is larger than the threshold value, the collected images are assumed to show the discharge state. Thus, the reference value is set for the discharge state and the non-discharge state.

Abstract: [Problem] Provision of a method for fractionating lipids using liquefied dimethyl ether as a solvent. [Means for Solving] To provide a method for fractionating a lipid using liquefied dimethyl ether as a solvent. [Solution] A method for fractionating a lipid, which comprises subjecting a microbial biomass to extraction using liquefied dimethyl ether as a solvent and then fractionating the lipid utilizing separation selectivity for the lipid. A method for producing a lipid, which comprises subjecting a microbial biomass to extraction using liquefied dimethyl ether as a solvent, then fractionating a portion of the lipid utilizing separation selectivity for the lipid to modify the fatty acid composition of the remainder of the lipid which remains in the microbial biomass, and then extracting the lipid having a modified fatty acid composition.

Abstract: To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.