Abstract: 1) A fused gene including a nucleic acid sequence which affects the biosynthesis or accumulation of neutral lipid and a phosphorus deficiency-responsive expression control sequence which is operably linked to the nucleic acid sequence and controls the expression of the nucleic acid sequence, 2) a transgenic plant which contains the fused gene, 3) a method for manufacturing vegetable fat or oil, including a cultivation step of cultivating the transgenic plant, and 4) a method for manufacturing vegetable fat or oil in which the cultivation step is a step of cultivating the transgenic plant in a phosphorus-deficient state are provided.

Abstract: 1) A fused gene including a nucleic acid sequence which affects the biosynthesis or accumulation of neutral lipid and a phosphorus deficiency-responsive expression control sequence which is operably linked to the nucleic acid sequence and controls the expression of the nucleic acid sequence, 2) a transgenic plant which contains the fused gene, 3) a method for manufacturing vegetable fat or oil, including a cultivation step of cultivating the transgenic plant, and 4) a method for manufacturing vegetable fat or oil in which the cultivation step is a step of cultivating the transgenic plant in a phosphorus-deficient state are provided.

Abstract: According to the present invention, a novel method for promoting fatty acid content in a plant is provided. In this method, the promoter of accD gene of acetyl-CoA carboxylase was replaced with a promoter directing abundant expression in plastids and chloroplasts by using plastid transformation. This method increases the carboxyltransferase beta subunit protein encoded by the accD gene. Accompanied with it, the other subunits constituting the acetyl-CoA carboxylase apparently increases and this enzyme increases. Since the acetyl-CoA carboxylase is a key enzyme for rate-limiting step of fatty acid synthesis, synthesis of fatty acid can be promoted by the method of the present invention. The transformed plant produced according to the method of the present invention exhibits remarkable enhancement in the fatty acid content in leaves and seeds. Moreover, the leaf longevity extended and the number of the seeds per a plant body increased, thereby seed oil and productivity of the plant are improved.