Abstract: A bisphenol composition includes 95% or more by mass of a bisphenol and 200 mass ppm or more of a compound represented by the following general formula (II): In formula (II), R21 and R22 denote a methyl group or a hydrogen atom; R22 is a methyl group when R21 is a hydrogen atom; R22 is a hydrogen atom when R21 is a methyl group; R23 to R25 independently denote a hydrogen atom or an alkyl group having 1 to 20 carbon atoms; and R23, R24, and R25 may be bonded or cross-linked between two of the groups. A method for producing a polycarbonate resin using the bisphenol composition is also described.

Abstract: A bisphenol composition including 95% or more by mass of a bisphenol, wherein a bisphenol represented by the following general formula (II) in the bisphenol composition constitutes 150 mass ppm or more, and the bisphenol composition has a methanol dissolution color (Hazen color number) of 2 or less, In formula (II), X denotes a single bond, —CR11R12—, —O—, —CO—, —S—, —SO—, or —SO2—, R11 and R12 independently denote a hydrogen atom or an alkyl group having 1 to 10 carbon atoms, and R11 and R12 may be bonded to each other to form a ring. A method for producing a polycarbonate resin using the bisphenol composition is also described.

Abstract: Provided is a simple and highly sensitive nucleic acid amplification method including hybridizing two types of oligonucleotide probes with a target gene and ligating the oligonucleotide probes with DNA ligase and amplifying the resultant single-stranded oligonucleotide in accordance with LAMP.

Abstract: Disclosed is a novel isothermal nucleic acid amplification method enabling inexpensive and simple and easy detection. The method includes introducing nicking enzyme recognition sequences into an analysis target nucleic acid using nicking enzyme recognition sequence-containing primers and isothermally amplifying a specific region of the target nucleic acid using the primers, a nicking enzyme and a DNA polymerase having strand displacement activity.

Abstract: According to the present invention, stable amplification of a small amount of nucleic acid and analysis of the same with good sensitivity can be realized by improving efficiency of hybridization primers or probes with a probe. Specifically, the present invention relates to a method of analyzing nucleic acid comprising: a step of hybridizing at least one type of a first probe comprising a 1st sequence complementary to one strand of double-strand nucleic acid, a 2nd sequence complementary to the other strand thereof with the double-strand nucleic acid, and a 3rd sequence that binds the 1st sequence and the 2nd sequence; and a step of hybridizing at least one type of a second probe with the double-strand nucleic acid.

Abstract: It is intended to provide a nucleic acid detection probe that is designed with a high degree of flexibility. The present invention provides a nucleic acid detection probe used in nucleic acid detection, wherein the amount of a particular target gene present in a sample is examined by simultaneously hybridizing, to the target nucleic acid, a first probe labeled at one end with a fluorophore and a second probe labeled with a quencher at an end different from the labeled end of the first probe and observing quenching attributed to the interaction between the fluorophore and the quencher.

Abstract: Provided is a simple and highly sensitive nucleic acid amplification method including hybridizing two types of oligonucleotide probes with a target gene and ligating the oligonucleotide probes with DNA ligase and amplifying the resultant single-stranded oligonucleotide in accordance with LAMP.

Abstract: The present invention relates to a technique for detecting the presence or absence of any mutation in a sample nucleic acid having plural possible mutation sites, which is necessary for identification of a cancerous cell.

Abstract: A method amplifies a nucleic acid by preparing an oligonucleotide being capable of complementarily hybridizing with a specific region of a target nucleic acid containing at least one mutation site, the oligonucleotide having at least one sequence being non-complementary to any of possible sequences of the at least one mutation site, subjecting the oligonucleotide to hybridization with the target nucleic acid, and carrying out a complementary-strand synthesis. A single-nucleotide polymorphism is analyzed using this method.