Abstract: A method of screening for a substance that acts on a cell mass includes producing a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue, adding a test substance to the cell mass, and evaluating an action of the test substance on the cell mass. The cell mass is produced by culturing cells obtained from the tumor tissue in a medium containing a 5% v/v or less extracellular matrix on a substantially low-adhesive cell culture substrate and producing the cell mass of the primary cancer cells.

Abstract: A method of screening for a substance that acts on a cell mass includes producing a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue, adding a test substance to the cell mass, and evaluating an action of the test substance on the cell mass. The cell mass is produced by culturing cells obtained from the tumor tissue in a medium containing a 5% v/v or less extracellular matrix on a substantially low-adhesive cell culture substrate and producing the cell mass of the primary cancer cells.

Abstract: A method of producing a cell mass by three-dimensional culture of primary cancer cells having proliferative ability and properties of handleability, versatility, and high-throughput performance, in which a tumor tissue is used as a starting material, proliferation of cells such as fibroblasts other than cancer cells is inhibited, and the cell mass includes primary cancer cells as a main component. The object is achieved by providing a method of producing a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue, including: a three-dimensional culture step of culturing cells obtained from the tumor tissue in a medium containing a 5% v/v or less extracellular matrix on a substantially low-adhesive cell culture substrate.

Abstract: A method of producing a cell mass by three-dimensional culture of primary cancer cells having proliferative ability and properties of handleability, versatility, and high-throughput performance, in which a tumor tissue is used as a starting material, proliferation of cells such as fibroblasts other than cancer cells is inhibited, and the cell mass includes primary cancer cells as a main component. The object is achieved by providing a method of producing a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue, including: a three-dimensional culture step of culturing cells obtained from the tumor tissue in a medium containing a 5% v/v or less extracellular matrix on a substantially low-adhesive cell culture substrate.

Abstract: The present application relates to a method for the targeted formation of heterochromatin and/or induction of epigenetic gene silencing in a cell using a small RNA, said method comprising the step of inhibiting the Paf1 complex in said cell and the step of contacting said cell with a small RNA targeted to a region of the genome of the cell, said region being the region where heterochromatin formation and/or induction of epigenetic gene silencing should be induced.

Abstract: The present application relates to a method for the targeted formation of heterochromatin and/or induction of epigenetic gene silencing in a cell using a small RNA, said method comprising the step of inhibiting the Paf1 complex in said cell and the step of contacting said cell with a small RNA targeted to a region of the genome of the cell, said region being the region where heterochromatin formation and/or induction of epigenetic gene silencing should be induced.

Abstract: One end of a terminal is formed in a tube shape, and a front end of a covered electric wire in which a part of a covering is peeled is inserted to the one end of the terminal, and is uniformly caulked from a periphery. A length of the peeled covering is shorter than a depth of the tube-like portion of the terminal. Accordingly, a part of the covering is received in the tube-like portion and caulked so as to serve as an operation of a packing, thereby preventing a water and an air from entering into an inner portion of the tube-like portion. Further, since the tube-like portion is uniformly caulked so as to be closely contact with the electric conductor, it is possible to obtain a comparatively large contact area, and it is possible to obtain a stable conduction.

Abstract: One end of a terminal is formed in a tube shape, and a front end of a covered electric wire in which a part of a covering is peeled is inserted to the one end of the terminal, and is uniformly caulked from a periphery. A length of the peeled covering is shorter than a depth of the tube-like portion of the terminal. Accordingly, a part of the covering is received in the tube-like portion and caulked so as to serve as an operation of a packing, thereby preventing a water and an air from entering into an inner portion of the tube-like portion. Further, since the tube-like portion is uniformly caulked so as to be closely contact with the electric conductor, it is possible to obtain a comparatively large contact area, and it is possible to obtain a stable conduction.