Patents by Inventor Yuko Fukuyama
Yuko Fukuyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240141423Abstract: [OBJECT] To enable a more sensitive and uniform detection of a molecular-related ion in an analysis of an RNA contained in a sample by means of MALDI mass spectrometry. [MEANS FOR SOLVING PROBLEM] A mixed matrix containing 2,4-dihydroxyacetophenone (DHAP) and 2,4,6-trihydroxyacetophenone monohydrate (THAP) is used as a matrix to analyze an RNA contained in a sample by a matrix-assisted laser desorption/ionization mass spectrometer.Type: ApplicationFiled: October 25, 2023Publication date: May 2, 2024Inventor: Yuko FUKUYAMA
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Publication number: 20240096612Abstract: The present invention provides a method for mass spectrometry of a negatively charged organic synthetic compound, and a matrix that can be used in said method. A method for mass spectrometry of a negatively charged organic synthetic compound, the method comprising using, as a liquid matrix, an ionic liquid comprising an amine ion and an acidic group-containing organic substance ion. For example, the amine is 3-aminoquinoline (3-AQ), and the acidic group-containing organic substance is p-coumaric acid (p-CA). The negatively charged organic synthetic compound to be analyzed is, for example, an organic synthetic polymer compound or a complex compound.Type: ApplicationFiled: September 22, 2021Publication date: March 21, 2024Applicant: SHIMADZU CORPORATIONInventors: Keisuke SHIMA, Yuko FUKUYAMA
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Publication number: 20230408513Abstract: A method for analyzing a microorganism including an identification step for determining which of Abony and Pakistan which are two serotypes of Salmonella bacteria is contained in a sample which contains either Abony or Pakistan, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample, or a method for analyzing a microorganism including an identification step for determining which of Minnesota, Infantis and Brandenburg which are three serotypes of Salmonella bacteria is contained in a sample which contains Minnesota, Infantis or Brandenburg, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample, or a method for analyzing a microorganism including an identification step for determining which of Schwarzengrund and Montevideo which are two serotypes of Salmonella baType: ApplicationFiled: August 21, 2023Publication date: December 21, 2023Applicant: SHIMADZU CORPORATIONInventor: Yuko FUKUYAMA
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Publication number: 20230393134Abstract: A method for analyzing a microorganism including an identification step for determining which of Abony and Pakistan which are two serotypes of Salmonella bacteria is contained in a sample which contains either Abony or Pakistan, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample, or a method for analyzing a microorganism including an identification step for determining which of Minnesota, Infantis and Brandenburg which are three serotypes of Salmonella bacteria is contained in a sample which contains Minnesota, Infantis or Brandenburg, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample, or a method for analyzing a microorganism including an identification step for determining which of Schwarzengrund and Montevideo which are two serotypes of Salmonella baType: ApplicationFiled: August 21, 2023Publication date: December 7, 2023Applicant: SHIMADZU CORPORATIONInventor: Yuko FUKUYAMA
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Patent number: 11774448Abstract: [OBJECT] To enable accurate identification of a specific serotype of Salmonella bacteria in a method for analyzing a microorganism using a MALDI-MS. [MEANS FOR SOLVING PROBLEM] The present invention is a method for analyzing a microorganism including an identification step for determining which of Abony and Pakistan which are two serotypes of Salmonella bacteria is contained in a sample which contains either Abony or Pakistan, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample, or a method for analyzing a microorganism including an identification step for determining which of Minnesota. Infantis and Brandenburg which are three serotypes of Salmonella bacteria is contained in a sample which contains Minnesota.Type: GrantFiled: April 16, 2021Date of Patent: October 3, 2023Assignee: SHIMADZU CORPORATIONInventor: Yuko Fukuyama
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Publication number: 20230243847Abstract: [Problem to be solved] To provide a method for identifying a marker for discriminating a microorganism based on a small amount of actual measurement data.Type: ApplicationFiled: June 2, 2020Publication date: August 3, 2023Applicant: SHIMADZU CORPORATIONInventor: Yuko FUKUYAMA
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Patent number: 11435352Abstract: In an analysis method of the present invention, a compound represented by the following formula (I) as a matrix is mixed into an analysis target sample and the mixture is subjected to matrix-assisted laser desorption ionization mass spectrometry. In the formula (I), R is an alkyl group having 3-11 carbon atoms. The analysis target sample is a substance for which whether or not ?-lactamase is contained is to be determined. Analysis targets include, for example, bacteria and an extract from bacteria.Type: GrantFiled: February 13, 2019Date of Patent: September 6, 2022Assignee: Shimadzu CorporationInventors: Tadayuki Iwase, Yoshinobu Manome, Yoshimitsu Mizunoe, Kouki Fujioka, Yuko Fukuyama
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Publication number: 20220003778Abstract: A method for analyzing a microorganism according to the present invention includes: a step of reading, from a mass spectrum obtained by a mass spectrometric analysis of a sample containing a microorganism, a mass-to-charge ratio or ratios m/z of a peak or peaks originating from 1-10 kinds of proteins selected from the group, consisting of 10 kinds of proteins of gns, YaiA, YibT, PPI, L25, L21, S8, L17, L15 and S7 as well as any combination of the 10 kinds of proteins; and an identification step configured to determine, based on the read mass-to-charge ratio or ratios, m/z, of the peak or peaks, whether or not the microorganism contained in the sample contains at least one of the following six serotypes of Salmonella bacteria: Abaetetuba, Anatum, Newport, Poona, Tallahassee and Vellore.Type: ApplicationFiled: April 16, 2021Publication date: January 6, 2022Applicant: SHIMADZU CORPORATIONInventor: Yuko FUKUYAMA
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Publication number: 20220003764Abstract: [OBJECT] To enable accurate identification of a specific serotype of Salmonella bacteria in a method for analyzing a microorganism using a MALDI-MS. [MEANS FOR SOLVING PROBLEM] The present invention is a method for analyzing a microorganism including an identification step for determining which of Abony and Pakistan which are two serotypes of Salmonella bacteria is contained in a sample which contains either Abony or Pakistan, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample, or a method for analyzing a microorganism including an identification step for determining which of Minnesota. Infantis and Brandenburg which are three serotypes of Salmonella bacteria is contained in a sample which contains Minnesota.Type: ApplicationFiled: April 16, 2021Publication date: January 6, 2022Applicant: SHIMADZU CORPORATIONInventor: Yuko FUKUYAMA
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Patent number: 11137398Abstract: In a method for analyzing a microorganism using a matrix assisted laser desorption/ionization mass spectrometer, a matrix-and-additive mixture solution prepared by mixing one or both of an alkylphosphonic acid and a surfactant with a matrix substance is used for matrix assisted laser desorption/ionization. Either an alkylphosphonic acid or a surfactant, or both of them are used as matrix additives and are mixed with the matrix substance beforehand to prepare a matrix-and-additive mixture solution. After a solution which contains a microorganism to be analyzed has been dropped onto a sample plate, the matrix-and-additive mixture solution is dropped onto that solution and dried to form a mixed crystal which contains both the constituents of the microorganism and the matrix substance. This crystal is used as a sample for MALDI-MS analysis. The sensitivity of analysis is thereby improved, without increasing the amount of time and labor required for sample preparation.Type: GrantFiled: February 12, 2019Date of Patent: October 5, 2021Assignee: SHIMADZU CORPORATIONInventors: Yuko Fukuyama, Hiroto Tamura, Teruyo Kato
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Patent number: 10626141Abstract: The present invention provides a method for specifically cleaving a C?-C bond of a peptide backbone and/or a side chain of a protein and a peptide, and a method for determining amino acid sequences of protein and peptide. A method for specifically cleaving a C?-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of 3-hydroxy-2-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, 5-hydroxy-2-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, and 4-hydroxy-2-nitrobenzoic acid. A method for determining an amino acid sequence of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of the above specific hydroxynitrobenzoic acid to specifically cleave a C?-C bond of a peptide backbone and/or a side chain bond, and analyzing generated fragment ions by mass spectrometry.Type: GrantFiled: May 12, 2017Date of Patent: April 21, 2020Assignees: SHIMADZU CORPORATION, NATIONAL UNIVERSITY OF CORPORATION HIROSHIMA UNIVERSITYInventors: Shunsuke Izumi, Koichi Tanaka, Yuko Fukuyama
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Publication number: 20200118650Abstract: Each of a plurality of mass spectral data that includes a microorganism of which strain is known is acquired as training data by a training data acquirer. A sample corresponding to each training data includes an additive, and a matrix is mixed with the sample. A discrimination analysis model for discriminating a strain based on the acquired plurality of training data is produced by a model producer by performing machine learning. Mass spectral data that includes a microorganism of which strain is unknown is acquired as target data by a target data acquirer. A sample corresponding to the target data includes the additive, and the matrix is mixed with the sample. A strain of the microorganism corresponding to the acquired target data is discriminated by a discriminator based on the produced discrimination analysis model for each strain and the acquired target data.Type: ApplicationFiled: October 7, 2019Publication date: April 16, 2020Applicant: Shimadzu CorporationInventors: Yoshihiro YAMADA, Yuko FUKUYAMA, Hiroto TAMURA
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Publication number: 20190369115Abstract: The present invention provides a mass spectrometry method using a mixed matrix, capable of easily and efficiently improving the ionization efficiency in mass spectrometry for peptides having a wide range of degrees of hydrophobicity (from hydrophilic to hydrophobic). A MALDI mass spectrometry method for analyzing a sample using as a mixed matrix, 2,4,6-trihydroxyalkylphenone represented by the following general formula (I): wherein R represents an alkyl group having 3 to 12 carbon atoms, and a matrix (II) for mass spectrometry that is more hydrophilic than 2,4,6-trihydroxyalkylphenone represented by the formula (I) wherein R is an alkyl group having 3 carbon atoms.Type: ApplicationFiled: May 31, 2018Publication date: December 5, 2019Applicant: SHIMADZU CORPORATIONInventors: Yuko FUKUYAMA, Koichi TANAKA
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Patent number: 10481163Abstract: The present invention provides a mass spectrometry method using a mixed matrix, capable of easily and efficiently improving the ionization efficiency in mass spectrometry for peptides having a wide range of degrees of hydrophobicity (from hydrophilic to hydrophobic). A MALDI mass spectrometry method for analyzing a sample using as a mixed matrix, 2,4,6-trihydroxyalkylphenone represented by the following general formula (I): wherein R represents an alkyl group having 3 to 12 carbon atoms, and a matrix (II) for mass spectrometry that is more hydrophilic than 2,4,6-trihydroxyalkylphenone represented by the formula (I) wherein R is an alkyl group having 3 carbon atoms.Type: GrantFiled: May 31, 2018Date of Patent: November 19, 2019Assignee: SHIMADZU CORPORATIONInventors: Yuko Fukuyama, Koichi Tanaka
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Publication number: 20190250169Abstract: In a method for analyzing a microorganism using a matrix assisted laser desorption/ionization mass spectrometer, a matrix-and-additive mixture solution prepared by mixing one or both of an alkylphosphonic acid and a surfactant with a matrix substance is used for matrix assisted laser desorption/ionization. Either an alkylphosphonic acid or a surfactant, or both of them are used as matrix additives and are mixed with the matrix substance beforehand to prepare a matrix-and-additive mixture solution. After a solution which contains a microorganism to be analyzed has been dropped onto a sample plate, the matrix-and-additive mixture solution is dropped onto that solution and dried to form a mixed crystal which contains both the constituents of the microorganism and the matrix substance. This crystal is used as a sample for MALDI-MS analysis. The sensitivity of analysis is thereby improved, without increasing the amount of time and labor required for sample preparation.Type: ApplicationFiled: February 12, 2019Publication date: August 15, 2019Applicant: Shimadzu CorporationInventors: Yuko FUKUYAMA, Hiroto TAMURA, Teruyo KATO
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Publication number: 20190250162Abstract: In an analysis method of the present invention, a compound represented by the following formula (I) as a matrix is mixed into an analysis target sample and the mixture is subjected to matrix-assisted laser desorption ionization mass spectrometry. In the formula (I), R is an alkyl group having 3-11 carbon atoms. The analysis target sample is a substance for which whether or not ?-lactamase is contained is to be determined. Analysis targets include, for example, bacteria and an, extract from bacteria.Type: ApplicationFiled: February 13, 2019Publication date: August 15, 2019Applicant: Shimadzu CorporationInventors: Tadayuki IWASE, Yoshinobu Manome, Yoshimitsu Mizunoe, Kouki Fujioka, Yuko Fukuyama
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Patent number: 9885725Abstract: The present invention provides a mass spectrometry method that can achieve analysis with high sensitivity using a matrix additive capable of improving ionization efficiency in mass spectrometry. A mass spectrometry method comprising the steps of: forming a mixed crystal for mass spectrometry on a target plate for mass spectrometry, the mixed crystal comprising a sample to be analyzed, a matrix, and a matrix additive selected from the group consisting of a 2,5-dihydroxybenzoate and a 3,5-dihydroxybenzoate that are represented by the following formula (I): where R represents an alkyl group having 4 to 14 carbon atoms; and irradiating the mixed crystal with laser to detect the sample to be analyzed.Type: GrantFiled: September 24, 2014Date of Patent: February 6, 2018Assignees: SHIMADZU CORPORATION, HIROSHIMA UNIVERSITYInventors: Yuko Fukuyama, Shunsuke Izumi
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Publication number: 20170327533Abstract: The present invention provides a method for specifically cleaving a C?-C bond of a peptide backbone and/or a side chain of a protein and a peptide, and a method for determining amino acid sequences of protein and peptide. A method for specifically cleaving a C?-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of 3-hydroxy-2-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, 5-hydroxy-2-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, and 4-hydroxy-2-nitrobenzoic acid. A method for determining an amino acid sequence of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of the above specific hydroxynitrobenzoic acid to specifically cleave a C?-C bond of a peptide backbone and/or a side chain bond, and analyzing generated fragment ions by mass spectrometry.Type: ApplicationFiled: May 12, 2017Publication date: November 16, 2017Inventors: Shunsuke Izumi, Koichi Tanaka, Yuko Fukuyama
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Patent number: 9453846Abstract: The present invention provides a mass spectrometry method using a matrix that is capable of easily and efficiently improving ionization efficiency in mass spectrometry without modifying a molecule to be analyzed, and a matrix for mass spectrometry. A mass spectrometry method using, as a matrix, a 2,4,6-trihydroxyalkylphenone represented by the following general formula (I): where R is an alkyl group having 4 to 12 carbon atoms. The mass spectrometry method as described above, wherein an analysis object is a hydrophobic compound, particularly, a hydrophobic peptide.Type: GrantFiled: March 4, 2014Date of Patent: September 27, 2016Assignees: SHIMADZU CORPORATION, NATIONAL UNIVERSITY OF CORPORATION HIROSHIMA UNIVERSITYInventors: Yuko Fukuyama, Shunsuke Izumi
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Publication number: 20160011205Abstract: The present invention provides amass spectrometry method using a matrix that is capable of easily and efficiently improving ionization efficiency in mass spectrometry without modifying a molecule to be analyzed, and a matrix for mass spectrometry. A mass spectrometry method using, as a matrix, a 2,4,6-trihydroxyalkylphenone represented by the following general formula (I): where R is an alkyl group having 4 to 12 carbon atoms. The mass spectrometry method as described above, wherein an analysis object is a hydrophobic compound, particularly, a hydrophobic peptide.Type: ApplicationFiled: March 4, 2014Publication date: January 14, 2016Applicant: Shimadzu CorporationInventors: Yuko Fukuyama, Shunsuke Izumi