Abstract: A nitrifying bacteria formulation that can cause a sufficient nitrification reaction even in a high ionic concentration (salt concentration), and efficiently collect high-concentration nitrate-form nitrogen from ammonia produced in animal husbandry facilities, composting facilities, sewage treatment plants, etc.; and a method for culturing bacteria to be contained in the nitrifying bacteria formulation. A nitrifying bacteria formulation includes: ammonia-oxidizing bacteria and nitrite-oxidizing bacteria, wherein the ammonia-oxidizing bacteria include bacteria belonging to the Nitrosococcus, the proportion of the number of the bacteria belonging to the Nitrosococcus with respect to the total number of bacteria in the nitrifying bacteria formulation is at least 0.1%, and the proportion of the number of the nitrite-oxidizing bacteria with respect to the total number of bacteria in the nitrifying bacteria formulation is at least 0.1%.

Abstract: A method for producing a calcium phosphate bone filler by preparing a mixed liquid comprising a calcium solution and a phosphoric acid solution in such a manner that a substrate of calcium phosphate exists in the mixed liquid, thereby precipitating calcium phosphate on the substrate to form a coating layer, and heat-treating the resultant composite comprising the substrate and the coating layer.

Abstract: A method for producing a calcium phosphate bone filler by preparing a mixed liquid comprising a calcium solution and a phosphoric acid solution in such a manner that a substrate of calcium phosphate exists in the mixed liquid, thereby precipitating calcium phosphate on the substrate to form a coating layer, and heat-treating the resultant composite comprising the substrate and the coating layer.