Abstract: Disclosed is a microarray printing system and methods of printing probe microarrays. The system has a print head formed of one or more bundles of individual capillaries, such as light-guiding capillaries. The bundles may especially be random bundles of capillaries that provide a large number of probes on the surface of a substrate. Methods of registering or correlating the distal and proximal ends of the capillaries are also provided. Further, the invention provides methods and equipment for identifying defective microarrays that are missing one or more probes from the surface of the microarray.

Abstract: Disclosed is a microarray printing system and methods of printing probe microarrays. The system has a print head formed of one or more bundles of individual capillaries, such as light-guiding capillaries. The bundles may especially be random bundles of capillaries that provide a large number of probes on the surface of a substrate. Methods of registering or correlating the distal and proximal ends of the capillaries are also provided. Further, the invention provides methods and equipment for identifying defective microarrays that are missing one or more probes from the surface of the microarray.

Abstract: Systems and methods of biochemical analysis are provided. A method of performing biochemical assays includes loading a first plurality of reservoirs on a first liquid carrier with a first plurality of compounds, coupling a second carrier with the first carrier, the second carrier having a second plurality of reservoirs configured to couple with the first plurality of reservoirs and containing a second plurality of compounds, transferring at least a portion of the first plurality of compounds to the second plurality of reservoirs, and separating at least one component from the second plurality of reservoirs. A system for biochemical analysis includes a first carrier and a second carrier. The first carrier includes a first substrate and a plurality of reservoirs in the substrate for retaining a first plurality of compounds.

Abstract: The invention provides methods and compositions relating to semaphorin K1 (sema K1) polypeptides which regulate cellular guidance and physiology, and related nucleic acids. The polypeptides may be produced recombinantly from transformed host cells from the disclosed sema K1 encoding nucleic acids or purified from human cells. The invention provides isolated sema K1 hybridization probes and primers capable of specifically hybridizing with the disclosed sema K1 genes, sema K1-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.

Abstract: Tandem pore domain weak inward rectifying K+ (TWIK) channel nucleic acids and proteins that have been isolated from Drosophila melanogaster and Leptinotarsa are described. The TWIK channel nucleic acids and proteins can be used to genetically modify metazoan invertebrate organisms, such as insects, coelomates, and pseudocoelomates, or cultured cells, resulting in TWIK channel expression or mis-expression. The genetically modified organisms or cells can be used in screening assays to identify candidate compounds which are potential pesticidal agents or therapeutics that interact with TWIK channel proteins. They can also be used in methods for studying TWIK channel activity and identifying other genes that modulate the function of, or interact with, the TWIK channel gene.

Abstract: The invention provides methods and compositions relating to semaphorin K1 (sema K1) polypeptides which regulate cellular guidance and physiology, and related nucleic acids. The polypeptides may be produced recombinantly from transformed host cells from the disclosed sema K1 encoding nucleic acids or purified from human cells. The invention provides isolated sema K1 hybridization probes and primers capable of specifically hybridizing with the disclosed sema K1 genes, sema K1-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.

Abstract: The invention provides methods and kits for detecting and/or quantifying nucleic acid sequences of interest by using pairs of probes containing donor-acceptor moieties that when hybridized on a target polynucleotide, with one of the probes being hybridized to a sequence of interest in the target polynucleotide, places the donor-acceptor moieties in sufficiently close proximity such that a detectable signal is generated. Methods of the invention are particularly useful for genotyping analysis and gene expression profiling. Methods of the invention are easily adaptable to arrays and automation. The invention also provides probes with enhanced hybridization efficiency and/or specificity comprising a probe portion having a spacer element and/or a minor groove binder molecule, and methods and kits for using these probes.

Abstract: Disclosed are systems and methods involved in extreme high throughput screening of compounds which have an affinity for a biological target. The system is based on a capillary bundle with two distinguishable ends wherein capillaries on one end are connected to compounds stored in discrete reservoirs and capillaries on the other end are bound and processed to form a two dimensional microarray. A capillary bundle having reaction wells for hybridization and compound reaction in one end of the capillaries is disclosed. The capillaries may be light-conducting. Also disclosed are various methods of identifying a target compound in a liquid using this capillary bundle as well as methods of fabricating the bundle.

Abstract: The inventions disclosed herein provide a novel 3-dimensional arrangement of probes on a probe carrier with concomitant increases in the economy of reagents, compactness and readability of the probe carriers. Novel methods of fabricating probe carriers are also disclosed. Techniques for hybridization assays and reading results of such assays are also presented.

Abstract: Disclosed is a microarray printing system and methods of printing probe microarrays. The system has a print head formed of one or more bundles of individual capillaries, such as light-guiding capillaries. The bundles may especially be random bundles of capillaries that provide a large number of probes on the surface of a substrate. Methods of registering or correlating the distal and proximal ends of the capillaries are also provided. Further, the invention provides methods and equipment for identifying defective microarrays that are missing one or more probes from the surface of the microarray.

Abstract: Disclosed are systems and methods involved in ultra high throughput screening of chemical compounds which have an affinity for or interact with a biological target. The invention utilizes an apparatus for ultra high throughput screening of chemical compounds for a biological target. The system is based on a capillary bundle that has two distinguishable ends. Capillaries on one end are connected to chemical compounds stored in discrete reservoirs such as micro titer plate wells; capillaries on the other end are tightly bound and then processed to form a two dimensional array that enable the parallel reactions of a target and many different compounds.

Abstract: A capillary bundle having reaction wells in one end of the capillaries is disclosed. The reaction wells serve as sites for hybridization, compound reaction, and drug identification for instance. The capillaries may be light-conducting capillaries. Also disclosed are various methods of identifying a target compound in a liquid carrier using this capillary bundle as well as methods of fabricating the bundle.

Abstract: Disclosed is a microarray printing system and methods of printing probe microarrays. The system has a print-head formed of one or more capillary bundle, such as light-guiding capillaries. The bundles may especially be bundles of capillaries that provide a large number of probes on the surface of a substrate. Methods of registering or correlating the distal and proximal ends of the capillaries are also provided. Further, the invention provides methods and equipment for identifying defective microarrays that are missing one or more probes from the surface of the microarray.

Abstract: The invention relates to a probe carrier in which a flexible substrate carries a one-dimensional configuration of probes wherein each different type of probe is attached to its own discrete portion of the substrate. The invention also relates to a probe carrier in which a flexible substrate such as a tape or fiber carries a two-dimensional configuration of probes. Furthermore, systems for fabricating and packaging flexible probe carrier threads are presented. Flexible probe carrier threads are packaged in forms of pins, rods, coils and spools to increase efficiency of hybridization and generate compact formats for transportation and use of probe carriers. Novel methods for hybridization of packaged probe carriers are disclosed. Methods for reading results of hybridization to packaged probe carriers are also disclosed.

Abstract: The invention provides methods and compositions relating to semaphorin K1 (sema K1) polypeptides which regulate cellular guidance and physiology, and related nucleic acids. The polypeptides may be produced recombinantly from transformed host cells from the disclosed sema K1 encoding nucleic acids or purified from human cells. The invention provides isolated sema K1 hybridization probes and primers capable of specifically hybridizing with the disclosed sema K1 genes, sema K1-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.

Abstract: Essentially pure human collapsin and its uses are disclosed. An isolated nucleic acid molecule that comprises a nucleotide sequence that is complementary to a nucleotide sequence that encodes a portion of human collapsin is disclosed. Antibodies that specifically bind to human collapsin and that inhibit its activity are disclosed. Methods of inhibiting the activity of collapsin, methods of inducing neurite outgrowth and methods of treating individuals suffering from nerve damage comprising the step of contacting neuronal cells with nucleic acid molecule that comprises a nucleotide sequence that is complementary to a nucleotide sequence that encodes a portion of human collapsin or comprising the step of contacting a neuronal cell with an antibody that specifically binds to human collapsin are disclosed. Methods of identifying compounds that inhibit the activity of human collapsin comprising the steps of contacting a neuronal cell with human collapsin in the presence of a test compound are disclosed.