Abstract: A method and apparatus designed for nucleic acid hybridization employs a hydrogen bond denaturation area with a higher temperature and a lower temperature nucleic acid hybridization area that is immobilized with nucleic acid probes. Nucleic acid-containing samples are introduced into the hydrogen bond denaturation area for heating curled nucleic acids in samples so that they become linear and are guided into the nucleic acid hybridization area. In this area, the nucleic acid hybridization rate can be multiplied by increasing the kinetic energy by the repeated flow of driven fluid. The nucleic acid hybridization apparatus provided by the invention contains a hydrogen bond denaturation area, a nucleic acid hybridization area, a two-way driving apparatus, and a temperature control element.

Abstract: A device for extracting nucleic acids includes a cover including a first opening and a second opening spaced apart from the first opening, a chip including a first terminal to communicate with the first opening and a second terminal to communicate with the second opening, and a flow channel of the chip including an inner surface onto which immobilized beads are dispersed for binding with nucleic acids in a fluid flowing in the flow channel.

Abstract: An RNA separation method includes injecting a reaction solution into a reaction region which contains a covalent linking of specific RNA probe for hybridizing with the reaction solution; adjusting a moderate hybridization temperature in the reaction region; moving the remainder of the reaction solution to a transition region by using a pneumatic actuator; adding a buffer solution to the reaction region, and heating the reaction region to a moderate denature temperature by a heating apparatus; moving a waste solution which is product produced from the reaction region after denature to a waste region by using the pneumatic actuator; and moving the remainder solution back to the reaction region by the pneumatic actuator.

Abstract: A device for extracting nucleic acids includes a cover including a first opening and a second opening spaced apart from the first opening, a chip including a first terminal to communicate with the first opening and a second terminal to communicate with the second opening, and a flow channel of the chip including an inner surface onto which immobilized beads are dispersed for binding with nucleic acids in a fluid flowing in the flow channel.

Abstract: This present invention is concerned with a device and method for the mRNAs purification of prokaryotic cells. The device based on a microfluidic chip which comprises: (a) a reaction region including the heater apparatus and the specific rRNA or/and tRNA probes for hybridization; (b) a transition region for stocking the remainder of reaction solution; (c) a water region for discarding the waste buffer solutions; and (d) a pneumatic actuator apparatus including the air compressor for blowing airflow and controlling the process of reaction.

Abstract: A method and apparatus designed for nucleic acid hybridization employs a hydrogen bond denaturation area with a higher temperature and a lower temperature nucleic acid hybridization area that is immobilized with nucleic acid probes. Nucleic acid-containing samples are introduced into the hydrogen bond denaturation area for heating curled nucleic acids in samples so that they become linear and are guided into the nucleic acid hybridization area. In this area, the nucleic acid hybridization rate can be multiplied by increasing the kinetic energy by the repeated flow of driven fluid. The nucleic acid hybridization apparatus provided by the invention contains a hydrogen bond denaturation area, a nucleic acid hybridization area, a two-way driving apparatus, and a temperature control element.

Abstract: A pneumatic driving device and the associated method for micro fluids, wherein the pneumatic driving device for micro fluids is constructed by connecting a fluid pipe with a structure that is formed by two gas stream inlets, a gas stream fender, a gas stream flow path and a gas stream outlet. The suction, exclusion and stagnation for the micro fluids inside the fluid pipes can be accomplished by adjusting the flow rates of the gas streams into the device at the two gas stream inlets. In the invention, several pneumatic driving devices are connected to form a single recursive pneumatic driving device by the concept of recursion, and the micro fluids inside several fluid pipes can be controlled to mix or to separate through the recursive pneumatic driving device by adjusting the flow rates of the gas streams into the device at different gas stream inlets.

Abstract: A pneumatic driving device and the associated method for micro fluids, wherein the pneumatic driving device for micro fluids is constructed by connecting a fluid pipe with a structure that is formed by two gas stream inlets, a gas stream fender, a gas stream flow path and a gas stream outlet. The suction, exclusion and stagnation for the micro fluids inside the fluid pipes can be accomplished by adjusting the flow rates of the gas streams into the device at the two gas stream inlets. In the invention several pneumatic driving devices are connected to form a single recursive pneumatic driving device by the concept of recursion, and the micro fluids inside several fluid pipes can be controlled to mix or to separate through the recursive pneumatic driving device by adjusting the flow rates of the gas streams into the device at different gas stream inlets.

Abstract: A pneumatic driving device and the associated method for micro fluids, wherein the pneumatic driving device for micro fluids is constructed by connecting a fluid pipe with a structure that is formed by two gas stream inlets, a gas stream fender, a gas stream flow path and a gas stream outlet. The suction, exclusion and stagnation for the micro fluids inside the fluid pipes can be accomplished by adjusting the flow rates of the gas streams into the device at the two gas stream inlets. In the invention several pneumatic driving devices are connected to form a single recursive pneumatic driving device by the concept of recursion, and the micro fluids inside several fluid pipes can be controlled to mix or to separate through the recursive pneumatic driving device by adjusting the flow rates of the gas streams into the device at different gas stream inlets.

Abstract: A method and apparatus designed for nucleic acid hybridization employs a hydrogen bond denaturation area with a higher temperature and a lower temperature nucleic acid hybridization area that is immobilized with nucleic acid probes. Nucleic acid-containing samples are introduced into the hydrogen bond denaturation area for heating curled nucleic acids in samples so that they become linear and are guided into the nucleic acid hybridization area. In this area, the nucleic acid hybridization rate can be multiplied by increasing the kinetic energy by the repeated flow of driven fluid. The nucleic acid hybridization apparatus provided by the invention contains a hydrogen bond denaturation area, a nucleic acid hybridization area, a two-way driving apparatus, and a temperature control element.

Abstract: The invention discloses a device for hybridization reaction between a target molecule in a fluid and a probe, which comprises a microfluidic channel comprising a first portion and a second portion following said first portion; wherein said first portion have an irregular cross section and said second portion has a probe, and a fluid driving element connected the ends of said channel with tubes, wherein said fluid element can move said target molecules back-and-forth for repeatedly passing through said second portion.

Abstract: Within each of a microfluidic mixer apparatus and a microfluidic reactor apparatus, as well as respective methods for operation thereof, there is provided a substrate having formed therein an aperture. Within the microfluidic mixer apparatus and its method of operation there is introduced into the aperture at least a pair of reagents through a pair of channels which terminate obliquely at the aperture, such as to provide a swirling mixing of the pair of reagents. Within the microfluidic reactor apparatus, the aperture comprises a first end portion contiguous with a middle portion in turn contiguous with a second end portion, where the middle portion, but not the first end portion or the second end portion, has at least one baffle intruding therein.

Abstract: An order-changing microfluid mixer comprises an extended microfluidic channel and a chamber structure that provides order-changing functions to microfluid when the microfluid passes through the chanter structure. The order-changing chamber has two openings connected to said microfluidic channel and divides said microfluidic channel into two sections. When a microfluid is driven from one section of the microfluidic channel to the other, the microfluid enters into the order-changing chamber and flows along a first side of the order-changing chamber, whereby portions of the microfluid positioned adjacent to the second side of the order-changing chamber remain unmoved.