Patents by Inventor Yuval Ebenstein
Yuval Ebenstein has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11845928Abstract: A method of DNA fragmentation is provided. The method comprising incubating a semi-solid biological sample which comprises the DNA with an auxiliary domain-directed nuclease having a binding affinity and selectivity to pre-defined sites in the DNA so as to yield a DNA fragment-of-interest, to thereby fragment the DNA.Type: GrantFiled: April 14, 2016Date of Patent: December 19, 2023Assignee: Tsinghua UniversityInventors: Ting Zhu, Yuval Ebenstein, Chunbo Lou, Wenjun Jiang, Xuejin Zhao, Tslil Gabrieli
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Publication number: 20230220459Abstract: Methods of detecting methylated CpG are provided. Accordingly, there is provided a method of determining CpG methylation status in a DNA sample, the method comprising: (a) subjecting the DNA sample to bisulfite conversion; (b) amplifying said DNA sample following said (a) to obtain an amplified DNA sample; (c) labeling CpG sites in said amplified DNA sample with a label to obtain a labeled DNA sample; (d) contacting said labeled DNA sample on an array comprising a plurality of probes for said DNA under conditions which allow specific hybridization between said plurality of probes and said DNA; and (e)detecting said hybridization, wherein an amount of said label is indicative of the CpG methylation status in said DNA sample.Type: ApplicationFiled: June 10, 2021Publication date: July 13, 2023Applicant: Ramot at Tel-Aviv University Ltd.Inventors: Yuval EBENSTEIN, Shahar ZIRKIN, Yael MICHAELI-HOCH, Noa GILAT, Orit FELDSTEIN, Adaya BEN-MOSHE
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Publication number: 20230047930Abstract: Methods and arrays for identifying the cell or tissue origin of DNA are provided. Accordingly there is provided a method of identifying DNA having a methylation pattern distinctive of a cell or tissue type or state comprising: labeling an epigenetic modification of interest in a DNA sample with a label; contacting said sample on an array comprising a plurality of probes for said DNA under conditions which allow specific hybridization between said plurality of probes and said DNA; and detecting said hybridization, wherein an amount of said label is indicative of the cell or tissue type or state, wherein the method is effected in the absence of amplification of said DNA.Type: ApplicationFiled: December 22, 2020Publication date: February 16, 2023Applicant: Ramot at Tel-Aviv University Ltd.Inventors: Yuval EBENSTEIN, Shahar ZIRKIN, Noa GILAT, Yael MICHAELI HOCH
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Patent number: 11427858Abstract: Methods and kits for detecting the presence of at least one target DNA sequence with or without a modification in a DNA molecule are provided.Type: GrantFiled: July 31, 2018Date of Patent: August 30, 2022Assignees: TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED, RAMOT AT TEL-AVIV UNIVERSITY LTD.Inventors: Amit Meller, Tal Gilboa, Chen Torfstein, Yuval Ebenstein, Elmar Weinhold, Yael Michaeli-Hoch, Assaf Grunwald
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Publication number: 20210371906Abstract: Methodologies for labeling the epigenetic modification 5-hydroxymethyl-cytosine (5hmC) along a DNA molecule, and for determining a presence or a level of this epigenetic modification based on a ratio of fluorescence intensity of a labeled DNA sample to absorption intensity of the DNA sample at 260 nm are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.Type: ApplicationFiled: August 12, 2021Publication date: December 2, 2021Applicant: Ramot at Tel-Aviv University Ltd.Inventors: Yuval EBENSTEIN, Micha FRIDMAN
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Publication number: 20210254167Abstract: A method of detecting an epigenetic DNA modification is described herein, the method comprising: attaching to DNA a labeling agent selective for the DNA modification; contacting an aqueous solution comprising the DNA with at least one addressable region on a surface of a substrate, wherein the addressable region comprises a positively charged substance capable of attaching the DNA to the surface, and the addressable region is surrounded by a hydrophobic region; and determining an amount of labeling agent in the addressable region. Further described herein is a kit comprising a substrate comprising discrete hydrophilic regions separated by a hydrophobic region, and a labeling agent selective for an epigenetic DNA modification; as well as an article of manufacture comprising a substrate comprising a discrete regions separated by a hydrophobic region, a positively charged substance and DNA attached thereto, the DNA comprising such a labeling agent.Type: ApplicationFiled: June 6, 2019Publication date: August 19, 2021Applicant: Ramot at Tel-Aviv University Ltd.Inventors: Yuval EBENSTEIN, Shahar ZIRKIN, Sapir MARGALIT, Yael MICHAELI HOCH
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Patent number: 11091793Abstract: Methodologies for labeling the epigenetic modification 5-hydroxymethylcytosine (5hmC) along a DNA molecule, and for determining a presence or a level of this epigenetic modification based on a ratio of fluorescence intensity of a labeled DNA sample to absorption intensity of the DNA sample at 260 nm are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.Type: GrantFiled: May 29, 2019Date of Patent: August 17, 2021Assignee: Ramot at Tel-Aviv University Ltd.Inventors: Yuval Ebenstein, Micha Fridman
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Publication number: 20200340032Abstract: Methods and kits for detecting the presence of at least one target DNA sequence with or without a modification in a DNA molecule are provided.Type: ApplicationFiled: July 31, 2018Publication date: October 29, 2020Inventors: Amit MELLER, Tal GILBOA, Chen TORFSTEIN, Yuval EBENSTEIN, Elmar WEINHOLD
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Publication number: 20200277649Abstract: The present invention relates to methods and compositions for detection of DNA damage, e.g., in a sample, cell or organism, that are amenable to high throughput, efficient and/or economical implementation, also capable of assessing a broad spectrum of DNA lesions.Type: ApplicationFiled: September 6, 2018Publication date: September 3, 2020Applicant: Ramot at Tel Aviv University, LtdInventors: Natalie Rose Gassmann, Nathaniel Holton, Yuval Ebenstein
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Publication number: 20200248265Abstract: A method of diagnosing a disease associated with a DNA repeat sequence is disclosed. The method comprises: (a) determining the number of repeats of a DNA sequence in DNA molecules of a sample of the subject; and (b) determining the CpG methylation status of the DNA molecules, wherein the number of repeats of the DNA sequence and the CpG methylation status of the DNA molecules is indicative of the disease.Type: ApplicationFiled: April 15, 2020Publication date: August 6, 2020Applicants: Ramot at Tel-Aviv University Ltd., Rheinisch-Westfälische Technische Hochschule (RWTH) AachenInventors: Yuval EBENSTEIN, Elmar WEINHOLD, Assaf GRUNWALD, Tslil GABRIELI
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Patent number: 10633706Abstract: A method of diagnosing a disease associated with a DNA repeat sequence is disclosed. The method comprises: (a) determining the number of repeats of a DNA sequence in DNA molecules of a sample of the subject; and (b) determining the CpG methylation status of the DNA molecules, wherein the number of repeats of the DNA sequence and the CpG methylation status of the DNA molecules is indicative of the disease.Type: GrantFiled: August 10, 2017Date of Patent: April 28, 2020Assignees: Ramot at Tel-Aviv University Ltd., Rheinisch-Westfälische Technische Hochschule (RWTH) AaachenInventors: Yuval Ebenstein, Elmar Weinhold, Assaf Grunwald, Tslil Gabrieli
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Publication number: 20190316178Abstract: Methodologies for labeling the epigenetic modification 5-hydroxymethyl-cytosine (5hmC) along a DNA molecule, and for imaging this epigenetic modification along a DNA molecule are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.Type: ApplicationFiled: May 29, 2019Publication date: October 17, 2019Applicant: Ramot at Tel-Aviv University Ltd.Inventors: Yuval EBENSTEIN, Micha FRIDMAN
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Patent number: 10351898Abstract: Methodologies for labeling the epigenetic modification 5-hydroxymethyl-cytosine (5hmC) along a DNA molecule, and for imaging this epigenetic modification along a DNA molecule are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.Type: GrantFiled: January 5, 2014Date of Patent: July 16, 2019Assignee: Ramot at Tel-Aviv University Ltd.Inventors: Yuval Ebenstein, Micha Fridman
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Publication number: 20190194755Abstract: A method of diagnosing a disease associated with a DNA repeat sequence is disclosed. The method comprises: (a) determining the number of repeats of a DNA sequence in DNA molecules of a sample of the subject; and (b) determining the CpG methylation status of the DNA molecules, wherein the number of repeats of the DNA sequence and the CpG methylation status of the DNA molecules is indicative of the disease.Type: ApplicationFiled: August 10, 2017Publication date: June 27, 2019Applicants: Ramot at Tel-Aviv University Ltd., Rheinisch-Westfalische Technische Hochschule (RWTH) AachenInventors: Yuval EBENSTEIN, Elmar WEINHOLD, Assaf GRUNWALD, Tslil GABRIELI
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Publication number: 20180327855Abstract: A method of detecting the level of 5-hydroxymethylcytosines (5-hmc) in a DNA molecule of a cell having a 5-hmc prevalence lower than 0.002% of total DNA bases is provided. The method comprising: (a) attaching a 5-hmc labeling agent to the DNA molecule; and (b) subjecting the DNA molecule to an imaging method suitable for detecting the labeling agent, thereby detecting the level of 5-hmc in the DNA molecule. Also provided is a method of diagnosing cancer in a subject in need thereof, the method comprising: (a) providing a DNA sample of a cell of the subject; (b) detecting the level of 5-hmc in the DNA sample as described herein; wherein a significant decrease in the level of 5-hmc in the DNA sample, as compared to a control DNA sample from a healthy subject is indicative that the subject has cancer.Type: ApplicationFiled: November 10, 2016Publication date: November 15, 2018Inventors: Yuval EBENSTEIN, Shahar ZIRKIN, Yael MICHAELI HOCH
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Publication number: 20180044659Abstract: A method of DNA fragmentation is provided. The method comprising incubating a semi-solid biological sample which comprises the DNA with an auxiliary domain-directed nuclease having a binding affinity and selectivity to pre-defined sites in the DNA so as to yield a DNA fragment-of-interest, to thereby fragment the DNA.Type: ApplicationFiled: April 14, 2016Publication date: February 15, 2018Inventors: Ting ZHU, Yuval EBENSTEIN, Chunbo LOU, Wenjun JIANG, Xuejin ZHAO, Tslil GABRIELI
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Publication number: 20160115525Abstract: Methodologies for labeling the epigenetic modification 5-hydroxymethyl-cytosine (5hmC) along a DNA molecule, and for imaging this epigenetic modification along a DNA molecule are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.Type: ApplicationFiled: January 5, 2014Publication date: April 28, 2016Inventors: Yuval EBENSTEIN, Micha FRIDMAN
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Patent number: 7528947Abstract: The present invention provides probes functionalized with nanoparticles, methods for binding nanoparticles to the probes and the use of such probes in nanometer scale imaging techniques. More specifically, the present invention provides a tip device having at least a portion thereof with an outer surface bound to at least one sub-layer of a material comprising nanoparticles, the nanoparticles acting as donors, acceptors, modifiers, quenchers and/or enhancers with respect to electromagnetic radiation.Type: GrantFiled: July 8, 2004Date of Patent: May 5, 2009Assignee: Yissum Research Development Company of the Hebrew University of JerusalemInventors: Uri Banin, Taleb Mokari, Yuval Ebenstein
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Publication number: 20070035724Abstract: The present invention provides probes functionalized with nanoparticles, methods for binding nanoparticles to the probes and the use of such probes in nanometer scale imaging techniques. More specifically, the present invention provides a tip device having at least a portion thereof with an outer surface bound to at least one sub-layer of a material comprising nanoparticles, the nanoparticles acting as donors, acceptors, modifiers, quenchers and/or enhancers with respect to electromagnetic radiation.Type: ApplicationFiled: July 8, 2004Publication date: February 15, 2007Applicant: Yissum Research Development Company of the Hebrew University of JerusalemInventors: Uri Banin, Taleb Mokari, Yuval Ebenstein