Abstract: The system comprises a master device (10) and at least one slave device (20). Each device has wireless transceiver and interface means (12, 22), a digital signal processor (14, 24), and a clock (16, 26). The master device transmits signals at a rate defined by its clock (16). The slave device has means for determining the frequency offset between the clocks and for correcting accordingly the rate at which signals are delivered internally to its processor. At the slave end, the frequency of the external clock(s) (26) is controllable about its nominal value, and means are provided for extracting a frequency offset value from a register (50) and for responding thereto by adjusting the frequency of the second clock in the direction tending to minimize the offset.

Abstract: The invention concerns products comprising a support whereon are fixed nucleic acids and their preparation method and use as DNA support. The invention also concerns functionalised supports, oligonucleotides and DNA's modified in position 5′ by a group selected in the group consisting of tartaric acid, serine, threonine, their derivatives and the &agr;-oxoaldehyde group, and the methods for preparing them. The invention further concerns a method for fixing a nucleic acid on a support.

Abstract: The invention relates to a functional DNA block by means of which hirudin may be prepared from yeast, which block contains at least the sequence:-S.sub.tr -L.sub.ex -S.sub.cl -H geneH gene being the gene coding for hirudin or one of its variants,S.sub.tr is a DNA sequence containing the signals which provide for the transcription of the H gene by yeast,L.sub.ex is a leader sequence needed in order to obtain the excretion of the gene product,S.sub.cl is a DNA sequence coding for the site of cleavage by yscF proteinase, which cleavage site is unique and situated in the hirudin precursor immediately upstream from the H gene.The invention relates more especially to a functional DNA block in which the H gene codes for HV1.

Abstract: The present invention relates to an improved system for producing mature heterologous proteins, and especially hirudin, by means of yeasts comprising an expression vector containing a sequence coding for the heterologous protein. This improvement is characterized by amplification of the KEX2 gene of yeast, coding for the endoprotease yscF. The amplification is carried out either by integration of one or more copies of all or part of the KEX2 gene in the yeast genome, or by insertion of one or more copies of all or part of the KEX2 gene into the vector for expression of the heterologous protein.

Abstract: Functional block of DNA enabling to prepare hirudine from yeast, characterized in that it comprises at least:--the gene of hirudine or one of its variants (gene H); --A DNA sequence (S.sub.tr) comprising the signals providing for the transcription of the gene H by the yeast.

Abstract: The present invention relates to variants of hirudin containing an amino acid different from the amino acid in the natural form at position 47 or 63.

Abstract: The invention relates to new DNA fragments and to their application as a DNA coding fragment for a signal peptide which can be used for the secretion of proteins, said peptide including a sequence of amino-acids which show a degree of correspondence of at least 60% with the sequence of amino-acids (I) or (II), preferably with the sequence (II). The sequences (I) and (II) are as follows: (I) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n. (II) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n-Val-Ser-Ala.

Abstract: The invention relates to a functional DNA block by means of which hirudin may be prepared from yeast, which block contains at least the sequence:--S.sub.tr --L.sub.ex --S.sub.cl --H geneH gene being the gene coding for hirudin or one of its variants,S.sub.tr is a DNA sequence containing the signals which provide for the transcription of the H gene by yeast,L.sub.ex is a leader sequence needed in order to obtain the excretion of the gene product,S.sub.cl is a DNA sequence coding for the site of cleavage by yscF proteinase, which cleavage site is unique and situated in the hirudin precursor immediately upstream from the H gene,The invention relates more especially to a functional DNA block in which the H gene codes for HV1.

Abstract: The present invention relates to an improved system for producing mature heterologous proteins, and especially hirudin, by means of yeasts comprising an expression vector containing a sequence coding for the heterologous protein. This improvement is characterized by amplification of the KEX2 gene of yeast, coding for the endoprotease yscF. The amplification is carried out either by integration of one or more copies of all or part of the KEX2 gene in the yeast genome, or by insertion of one or more copies of all or part of the KEX2 gene into the vector for expression of the heterologous protein.

Abstract: The invention relates to a new strain of Saccharomyces cerevisiae productive of a heterologous protein, and more especially of hirudin, characterized by a suppression of the proteolytic function encoded by the PRC1 gene. More specifically, this deficiency is due to a mutation or more especially to a deletion of the PRC1 structural gene.

Abstract: The present invention relates, in particular, to a DNA sequence corresponding to one of the following genes involved in the chain of biotin biosynthesis in bacteria: bioA gene, bioD gene, bioF, bioC and bioH gene.Vectors containing these sequences enable other microorganisms to be transformed in order to improve the production of biotin.