Abstract: Integrated cartridges for sample homogenization, nucleic acid fragmentation, and nucleic acid detection are disclosed herein. The integrated cartridges include a main housing having a sample well and a detection chamber and a sonication feature coupled to and extending outwardly from the main housing. The sonication feature includes a sonication chamber for receiving a sample fluid. A fluidic path directs the sample fluid from the sample well, to the sonication chamber, and to the detection chamber. Systems for use with the integrated cartridges can include sonotrodes with openings for positioning the sonication feature and/or temperature sensors for monitoring the temperature of the sonication feature. Methods include moving the sample fluid from the sample well to the sonication feature, transmitting ultrasonic energy into the sample fluid, moving the sample fluid from the sonication feature to the detection chamber, and performing a nucleic acid detection assay within the detection chamber.

Abstract: Disclosed herein are methods, devices and compositions comprising nucleic acid captor molecules with a stem region and a loop region for detecting target nucleic acids.

Abstract: Systems and methods are disclosed herein for determining the sensitivity of a target to a therapeutic formula. The systems include a measurement device for measuring properties of a target sample fluid housed within a cartridge (the cartridge having at least one test compartment and at least one control compartment). Methods can include determining the concentration of the target suspended in a target sample fluid and placing the target sample fluid into at least one test compartment comprising a first therapeutic formula. The system, via a processor, measures the properties of the sample fluid, compares the measured properties to a threshold property, determines the sensitivity of a target species to a therapeutic formula, and presents an indicator of the target sensitivity to an end user. In some embodiments, the sensitivity data can be stored to a database for later use by an adaptive machine learning tool.

Abstract: Disclosed herein are methods, devices and compositions comprising nucleic acid captor molecules with a stem region and a loop region for detecting target nucleic acids.