Abstract: Disclosed herein are a microorganism with excellent deacetoxycephalosporin C (DAOC) productivity and a use thereof. A mutant microorganism with improved DAOC productivity and a use thereof for producing 7-aminodeacetoxycephalosporanic acid (7-ADCA) are provided.

Abstract: A method includes providing a cartridge and the cartridge includes a slot for receiving a microfluidic chip having a set of first channels. The cartridge also includes a set of second channels and each channel of the set of second channels is coupleable to a different channel of the set of first channels during use with the microfluidic chip. The cartridge also includes an indent configured for engagement and alignment of the cartridge during use. The method also includes inserting the cartridge into a device, such that the cartridge engages a first biasing member of the device configured for alignment of the cartridge in a first direction. The first biasing member is configured to bias movement of the cartridge into locking position with a notch of the device.

Abstract: The present invention relates to the field of biomedical technology and discloses a cartridge and a testing device. The cartridge comprises a sample lysis compartment, a first sample mixing compartment and a first PCR compartment; a first valve is disposed between the sample lysis compartment and the first sample mixing compartment, the first valve controls the flowing or blocking of the sample between the sample lysis compartment and the first sample mixing compartment; a fourth valve is disposed between the first PCR compartment and the first sample mixing compartment, the fourth valve controls the flow or blocking of the sample between the first sample mixing compartment and the first PCR compartment; a first reagent is provided in the first sample mixing compartment. In the compartment, nucleic acids in the sample mix with the first reagent and is then sent to the first PCR compartment for amplification.

Abstract: A method includes providing a cartridge and the cartridge includes a slot for receiving a microfluidic chip having a set of first channels. The cartridge also includes a set of second channels and each channel of the set of second channels is coupleable to a different channel of the set of first channels during use with the microfluidic chip. The cartridge also includes an indent configured for engagement and alignment of the cartridge during use. The method also includes inserting the cartridge into a device, such that the cartridge engages a first biasing member of the device configured for alignment of the cartridge in a first direction. The first biasing member is configured to bias movement of the cartridge into locking position with a notch of the device.

Abstract: A system includes a sorting chamber and a fluid channel in fluid communication with the sorting chamber. A peristaltic pump is in fluid communication with the fluid channel to pump fluid through the fluid channel to the sorting chamber at a fluid flow rate. A fluid damper is in fluid communication with the sample fluid channel. The fluid damper includes a gas and reduces variations in the fluid flow rate by compression and expansion of the gas in response to fluid flow in the fluid channel.

Abstract: A system for pushing orders is disclosed. The system may include: a computer readable storage medium configured to store an executable module, comprising a service requester interface configured to: acquire at least one first order and information related to the first order, comprising geographic and time information related to the first order; and acquire at least one second order and information related to the second order, comprising geographic and time information related to the second order; a processing module configured to filter the second order based on the information related to the first order and the second order to generate at least one filtered second order; a service provider interface configured to send the filtered second order; and a processor configured to implement the executable module. Also disclosed herein are a method of pushing orders and a method of using a system for pushing orders.

Abstract: Techniques, devices and systems are disclosed for characterizing particles in a fluid sample by optical space-time coding. In one aspect, a microfluidic device for optical detection of particles includes a substrate, a microfluidic channel formed on the substrate and structured to carry a fluid sample containing particles, in which the microfluidic channel is structured to transmit a probe light, and a mask formed on one side of the microfluidic channel and structured to include a pattern of openings along the microfluidic channel, in which at least two of the openings have varying dimensions across the microfluidic channel, and in which the pattern of openings encodes a waveform on the probe light that transmits through the microfluidic channel to allow optical detection of a position of a particle in the microfluidic channel.

Abstract: Techniques, devices and systems are disclosed for characterizing particles in a fluid sample by optical space-time coding. In one aspect, a microfluidic device for optical detection of particles includes a substrate, a microfluidic channel formed on the substrate and structured to carry a fluid sample containing particles, in which the microfluidic channel is structured to transmit a probe light, and a mask formed on one side of the microfluidic channel and structured to include a pattern of openings along the microfluidic channel, in which at least two of the openings have varying dimensions across the microfluidic channel, and in which the pattern of openings encodes a waveform on the probe light that transmits through the microfluidic channel to allow optical detection of a position of a particle in the microfluidic channel.