Abstract: An ultrahigh-pressure acid-fracturing isodiametric hose assembly, comprising a hose body and a metal joint. The metal joint comprises an inner tube, a first outer tube the inner tube, and a second outer tube. The first outer tube and the second outer tube each is an independent pressing part. An external groove is provided at an outer wall of the inner tube. An end of the first outer tube comprises an internal ridge which matches the external groove for snapping. The terminal of the inner tube comprises a connecting portion. An outer wall of the inner tube comprises a first protrusion, and an inner wall of the first outer tube comprises a second protrusion engaging with the first protrusion. The hose body is clamped between the first protrusion and the second protrusion that are engaged. An inner wall of the second outer tube comprises multiple micro-protrusions.

Abstract: A transformer comprises a winding structure which forms a primary side coil, a secondary side coil and first and second wires wound into the coil structure of the transformer. The first wire and second wire define a capacitance between them. The first wire is coupled to a cold point in the primary side and the second wire is coupled to a cold point in the secondary side, thereby this capacitance forming a Y-capacitor between the primary and the secondary sides. This capacitance may for example be used for EMI suppression.

Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.

Abstract: A digital-to-analog conversion circuit, comprising: an R?2R resistive network (10) configured to be connected between an output end and a ground end; an output voltage selection unit (20) configured to be connected between the output end of the R?2R resistive network (10) and a voltage output terminal; an output voltage trimming unit (30), wherein the output voltage trimming unit (30) is provided between a 2R resistor on at least one branch of the R?2R resistive network (10) and the ground end.

Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.

Abstract: Amino acid substitutions that provide for increased elongation rates, resistance to PCR inhibitors, and reverse transcriptase activity of Thermus aquaticus (Taq) DNA polymerase enzymes are provided. Also provided are related methods of using the Taq DNA polymerase enzymes to rapidly detect nucleic acids of interest in crude biological samples, without DNA/RNA extraction.

Abstract: A digital-to-analog conversion circuit, comprising: an R?2R resistive network (10) configured to be connected between an output end and a ground end; an output voltage selection unit (20) configured to be connected between the output end of the R?2R resistive network (10) and a voltage output terminal; an output voltage trimming unit (30), wherein the output voltage trimming unit (30) is provided between a 2R resistor on at least one branch of the R?2R resistive network (10) and the ground end.

Abstract: The present application discloses a trimming circuit of differential amplifier, wherein an output end of the differential amplifier is coupled to a first input end of the differential amplifier through a first voltage-dividing resistor; a shift voltage is coupled to a second input end of the differential amplifier through a second voltage-dividing resistor; the first voltage-dividing resistor and the second voltage-dividing resistor respectively form a T-shaped resistor network structure; the T-shaped resistor network structure comprises: a k-bit resistive network coupled to a T-shaped node and a reference power supply end, wherein a low n-bits of the k-bit resistive network is an R-2R resistive network, and part of branches are connected in series with at least one trimming resistor, and each trimming resistor is connected in parallel with a switch.

Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.

Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.

Abstract: Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such.

Abstract: Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such.

Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.

Abstract: The present invention generally relates to detection of a target nucleic acid in standard PCR, real-time PCR, RT PCR, and real-time RT PCR. One aspect of the invention provides mutant DNA polymerase enzymes that are resistant to PCR inhibitors, such as dye, blood, and soil. Another aspect of the invention provides for methods of real-time PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing dye, blood, and/or soil. Another aspect of the invention provides for methods of standard PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing blood and/or soil.

Abstract: The present invention generally relates to detection of a target nucleic acid in standard PCR, real-time PCR, RT PCR, and real-time RT PCR. One aspect of the invention provides mutant DNA polymerase enzymes that are resistant to PCR inhibitors, such as dye, blood, and soil. Another aspect of the invention provides for methods of real-time PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing dye, blood, and/or soil. Another aspect of the invention provides for methods of standard PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing blood and/or soil.

Abstract: Provided herein are compositions and methods for improving amplification or detection of a target nucleic acid in a sample containing PCR inhibitors, such as polyphenols. One aspect provides an enhancer composition including casein or polyvinylpyrrolidone, or a modified polymer thereof. Another aspect provides a method of amplifying a target nucleic acid with an enhancer composition including casein or polyvinylpyrrolidone, or a modified polymer thereof. Another aspect provides a method for culturing microorganisms in media containing PVP or casein.

Abstract: Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such.

Abstract: The present invention generally relates to amplfication reactions. One aspect of the invention provides amplification reaction enhancer compositions comprising trehalose, carnitine, and a non-ionic detergent, such as NP40. These enhancer compositions can improve efficiency, specificity, and sensitivity of amplification reactions in conventional and real-time PCR and RT-PCR. In addition, these compositions permit nucleic acid amplification directly in crude samples containing blood, blood components, or soil extract with little or no nucleic acid extraction prior to amplification. Another aspect of the invention provides a method of enhancing an amplification reaction containing a crude blood sample with heparin. Another improvement derived from the invention is improved detection of difficult, high GC content nucleic acid targets.

Abstract: The present invention generally relates to detection of a target nucleic acid in standard PCR, real-time PCR, RT PCR, and real-time RT PCR. One aspect of the invention provides mutant DNA polymerase enzymes that are resistant to PCR inhibitors, such as dye, blood, and soil. Another aspect of the invention provides for methods of real-time PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing dye, blood, and/or soil. Another aspect of the invention provides for methods of standard PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing blood and/or soil.