Patents by Inventor Zhifeng Shao
Zhifeng Shao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11415494Abstract: A method of rapidly and completely rendering a tissue (11) rich in lipid droplets (12) transparent. The method comprises the following steps: providing a tissue sample (11) rich in lipid droplets (12) and immobilized with a hydrogel; performing a pre-transparency-rendering process on the tissue sample (11) to obtain a pre-processed sample; performing a transparency-rendering process on the pre-processed sample to obtain a transparency-rendered sample; and performing a post-transparency-rendering process on the transparency-rendered sample to obtain a final transparency-rendered sample (31). The method does not damage the fine structure of a biological tissue (11) and can significantly increase a depth of an optical image of the biological tissue (11). The method does not damage the fine structure of a biological tissue (11) and can significantly increase a depth of an optical image of the biological tissue (11).Type: GrantFiled: January 19, 2018Date of Patent: August 16, 2022Assignee: SHANGHAI JIAOTONG UNIVERSITYInventors: Xiaowei Li, Zhifeng Shao, Mengjie Lai, Jun Li, Daniel Czajkowsky
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Publication number: 20210072124Abstract: A method of rapidly and completely rendering a tissue (11) rich in lipid droplets (12) transparent. The method comprises the following steps: providing a tissue sample (11) rich in lipid droplets (12) and immobilized with a hydrogel; performing a pre-transparency-rendering process on the tissue sample (11) to obtain a pre-processed sample; performing a transparency-rendering process on the pre-processed sample to obtain a transparency-rendered sample; and performing a post-transparency-rendering process on the transparency-rendered sample to obtain a final transparency-rendered sample (31). The method does not damage the fine structure of a biological tissue (11) and can significantly increase a depth of an optical image of the biological tissue (11). The method does not damage the fine structure of a biological tissue (11) and can significantly increase a depth of an optical image of the biological tissue (11).Type: ApplicationFiled: January 19, 2018Publication date: March 11, 2021Inventors: Xiaowei LI, Zhifeng SHAO, Mengjie LAI, Jun LI, Daniel CZAJKOWSKY
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Patent number: 8481266Abstract: The present invention relates to the field of gene engineering, provides a DNA sequencing method and system. Said DNA sequencing method includes following steps: A. said DNA is processed into multiple DNA segments, and then constructed into multiple DNA tags; B. amplification of every single DNA tag, and then processed into single stranded DNA; C. Utilize the anchor which can ligate to DNA tags and possesses at least one degenerated base to sequence every single DNA tag and thus produce sequencing signal; D. Obtain sequences of every single DNA segment by sequencing signal. Said DNA sequencing system, includes: unit of short tags construction, unit of amplification, unit of sequencing reaction and unit of signal processing. In this invention, since DNA tags are sequenced by using sequencing anchor with at least one degenerative abase, length of DNA tags that can be directly sequenced is extended. Both short and long DNA tags can be sequenced. Thus application of DNA sequencing is expanded.Type: GrantFiled: November 17, 2008Date of Patent: July 9, 2013Assignee: Hyk Gene Technology Co., Ltd.Inventors: Zhifeng Shao, Sitong Sheng
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Publication number: 20110045992Abstract: The present invention relates to the field of gene engineering, provides a DNA sequencing method and system. Said DNA sequencing method includes following steps: A. said DNA is processed into multiple DNA segments, and then constructed into multiple DNA tags; B. amplification of every single DNA tag, and then processed into single stranded DNA; C. Utilize the anchor which can ligate to DNA tags and possesses at least one degenerated base to sequence every single DNA tag and thus produce sequencing signal; D. Obtain sequences of every single DNA segment by sequencing signal. Said DNA sequencing system, includes: unit of short tags construction, unit of amplification, unit of sequencing reaction and unit of signal processing. In this invention, since DNA tags are sequenced by using sequencing anchor with at least one degenerative abase, length of DNA tags that can be directly sequenced is extended. Both short and long DNA tags can be sequenced. Thus application of DNA sequencing is expanded.Type: ApplicationFiled: November 17, 2008Publication date: February 24, 2011Applicant: HYK GENE TECHNOLOGY CO., LTD.Inventors: Zhifeng Shao, Sitong Sheng
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Publication number: 20070148690Abstract: This invention relates generally to analysis of gene expression profiles. In particular, the present invention provides a method for a method for analyzing gene expression profiles of a cell, which method comprises: a) providing for isolated mRNA or cDNA target sequences from a cell; b) sequentially hybridizing said isolated mRNA or cDNA target sequences with a plurality of nucleotide probes; and c) assessing the sequential hybridization between said isolated mRNA or cDNA target sequences and said plurality of nucleotide probes to analyze gene expression profiles of said cell. Systems for analyzing gene expression profiles are also provided. Optical devices for detecting hybridization signal are further provided.Type: ApplicationFiled: February 6, 2007Publication date: June 28, 2007Inventors: Zhifeng Shao, Sitong Sheng, Shoudan Liang
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Patent number: 7189509Abstract: This invention relates generally to analysis of gene expression profiles. In particular, the present invention provides a method for a method for analyzing gene expression profiles of a cell, which method comprises: a) providing for isolated mRNA or cDNA target sequences from a cell; b) sequentially hybridizing said isolated mRNA or cDNA target sequences with a plurality of nucleotide probes; and c) assessing the sequential hybridization between said isolated mRNA or cDNA target sequences and said plurality of nucleotide probes to analyze gene expression profiles of said cell. Systems for analyzing gene expression profiles are also provided. Optical devices for detecting hybridization signal are further provided.Type: GrantFiled: August 16, 2002Date of Patent: March 13, 2007Inventors: Zhifeng Shao, Sitong Sheng, Shoudan Liang
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Patent number: 6621079Abstract: The present invention near field scanning optical microscope NSOM, and related method thereof, provides a high resolution image of a sample in aqueous solution without damaging the sample. This attribute will greatly expand the applications and utility of a NSOM in biomedicine, among other fields. Moreover, the NSOM can be further extended to include signals other than light. In operation, a pipette is filled with an electrolyte solution (aqueous solution) and lowered through the reservoir toward the surface of the sample while the current between the electrode inside the pipette and the electrode in the reservoir is monitored. As the tip of the pipette approaches the surface, the ion current decreases because the space through which ions can flow is reduced. The pipette is then scanned laterally over the surface and the path of the tip pipette follows the topography of the surface.Type: GrantFiled: June 29, 2000Date of Patent: September 16, 2003Assignee: University of Virginia Patent FoundationInventors: Zhifeng Shao, Gabor Szabo, Anders Mannelqvist
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Publication number: 20030087279Abstract: This invention relates generally to analysis of gene expression profiles. In particular, the present invention provides a method for a method for analyzing gene expression profiles of a cell, which method comprises: a) providing for isolated mRNA or cDNA target sequences from a cell; b) sequentially hybridizing said isolated mRNA or cDNA target sequences with a plurality of nucleotide probes; and c) assessing the sequential hybridization between said isolated mRNA or cDNA target sequences and said plurality of nucleotide probes to analyze gene expression profiles of said cell. Systems for analyzing gene expression profiles are also provided. Optical devices for detecting hybridization signal are further provided.Type: ApplicationFiled: August 16, 2002Publication date: May 8, 2003Inventors: Zhifeng Shao, Sitong Sheng, Shoudan Liang
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Patent number: 5834644Abstract: An atomic force microscope which can provide an automatic operation of focusing a laser beam onto a cantilever of the atomic force microscope, and which can ensure that a laser beam reflected off this cantilever is properly directed to a detector. A tip supported by the cantilever can also be automatically and properly lowered to a specimen. A piezotube scanner within such an atomic force microscope is also provided to prevent any damage from occurring if any leakage of an aqueous solution containing the specimen occurs.Type: GrantFiled: November 13, 1996Date of Patent: November 10, 1998Assignee: The University of Virginia Patent FoundationInventors: Zhifeng Shao, Jian Xun Mou, Gang Huang
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Patent number: 5079428Abstract: A magnetic electron focusing lens including a first pole piece, from a back side of which low-energy electrons are injected, and a second pole piece located on a front side of the first pole piece. The first and second pole pieces are coupled to form a single magnetic circuit. The first pole piece is shaped to maximize the magnetic field at a its front side at a peak much closer to the first then to the second pole piece. The specimen is located between the first and second pole pieces near the peak of the magnetic field.Type: GrantFiled: August 31, 1989Date of Patent: January 7, 1992Assignees: Bell Communications Research, Inc., Arch Development Corp.Inventors: Paul S. Da Lin, Zhifeng Shao