Abstract: A flexible electroluminescent fiber for embroidery sequentially includes: metal core wires, a light-emitting layer, a transparent conductive layer, a filament, and a protective paint, wherein a quantity of the metal core wires is an even number, and the metal core wires are pasted together before being wrapped by the light-emitting layer; the light-emitting layer is coated with the transparent conductive layer; the protective paint and the filament are exterior to the transparent conductive layer; the metal core wires emit light through energizing; a diameter of the electroluminescent fiber is 0.1-0.3 mm, and a 20-36V safe voltage is applied for emitting light. The flexible electroluminescent fiber of the present invention has sufficient tensile force, and smooth and soft surface. Appearance and hand feeling of the present invention are the same as those of clothing textile fibers.

Abstract: A bottom touching assisting device suitable for deep-sea submersibles and an implementation method thereof are provided. Four support columns are arranged within a mounting box body at a bottom of a deep-sea submersible, the support columns and the mounting box body are connected in a sliding manner through sliders, and the support column is sleeved with a threaded sleeve in a threaded connection manner. In conjunction with a drive component and a pressing mechanism, smooth vertical movement of the support column is achieved when the threaded sleeve rotates. This allows a bottom end of the support column to extend from the mounting box body.

Abstract: In an embodiment, the present disclosure pertains to a method of treating or preventing C. difficile infections. In some embodiments, the method includes administering an antitoxin to a subject in need thereof. In some embodiments, the antitoxin includes a designed ankyrin repeat protein (DARPin). In an additional embodiments, the present disclosure pertains to a composition including an antitoxin for treating or preventing C. difficile infections. In some embodiments, the anti-toxin includes a DARPin. In some embodiments, the anti-toxin is a monomeric or dimeric DARPin for the neutralization of Clostridium difficile toxin B (TcdB).

Abstract: The present invention provides a method for detecting the anti-island protection performance of an inverter. An RLC circuit is serially connected between the inverter and a grid connection switch, the resonant frequency of the RLC load is set as three different numerical values for enabling the same to present resistance, capacitance and inductance, and a time value from the disconnection of the grid connection switch to the operation stop of the inverter under detection is recorded after a fundamental current value of the grid connection switch is set to a different value each time; and weighted calculation is performed on the time value to obtain a test result and grade evaluation of the anti-island protection ability of the inverter.

Abstract: The present invention includes methods, compositions, uses, and kits for purifying a protein of interest (POI) comprising contacting a first fusion protein comprising the POI fused to the C-terminus of an intein C-fragment with a second fusion protein comprising an intein N-fragment and a purification tag to form a complex between the first fusion protein and the second fusion protein, cleaving the POI from the intein C-fragment, wherein the protein is released from the complex; and isolating the POI; the present invention also includes fusion proteins and vector.

Abstract: The present invention includes methods, compositions, uses, and kits for purifying a protein of interest (POI) comprising contacting a first fusion protein comprising the POI fused to the C-terminus of an intein C-fragment with a second fusion protein comprising an intein N-fragment and a purification tag to form a complex between the first fusion protein and the second fusion protein, cleaving the POI from the intein C-fragment, wherein the protein is released from the complex; and isolating the POI; the present invention also includes fusion proteins and vector.

Abstract: In an embodiment of the present disclosure there is provided a method of inactivating enveloped DNA virus particles in a biological sample. In an embodiment, such a method comprises contacting the biological sample with an effective amount of an antiviral composition comprising PD 404,182 In some embodiments, the DNA virus is the Herpes Simplex virus-1 (HSV-1) or the Herpes Simplex Virus-2 (HSV-2). In another embodiment of the present disclosure, there is provided a method of treating, preventing, or reducing a viral infection caused by an enveloped DNA virus in a subject in need thereof. In yet another embodiment, there is provided a method of preventing transmission of a viral infection to a subject in need thereof.

Abstract: In some embodiments the present disclosure provides a method of inactivating a RNA virus of the family retroviridae in a biological sample. Such a method comprises contacting the biological sample with an effective amount of an antiviral composition comprising PD 404,182. In an embodiment of the present disclosure the RNA virus is selected from the group consisting of HIV pseudotyped lentiviruses, primary human immunodeficiency virus-1 isolates (HIV-1), human immunodeficiency virus-2 (HIV-2), and simian immunodeficiency virus (SIV). Further embodiments of the present disclosure pertain to a method of treating a subject infected by a RNA virus of the family retroviridae. Another embodiment of the present invention pertains to a method of preventing transmission of a RNA virus of the family retroviridae in a subject in need thereof.

Abstract: The present invention relates to compositions and a cell-based assay for detecting endonuclease activity. The assay involves the basic principle of linking a endonuclease cleavage event with cell survival. When an endonuclease cleaves its cognate endonuclease recognition site located on a vector containing a toxic reporter protein, the vector is degraded and the cell survives because the toxic reporter protein cannot be produced. A cell employed in the instant assay advantageously expresses a transporter protein that facilitates transport of a regulatory molecule used to induce expression of the toxic reporter protein. Kits comprising the compositions of the instant invention are also provided.

Abstract: The present invention relates to compositions and methods for generating proteins with novel functions. The methods employ an in vitro coevolution approach that, in a stepwise manner, generates one or more intermediate functions. A pathway containing one or more analog molecules corresponding to the intermediate functions, and a target molecule, corresponding to the target function are designed and used to select mutant proteins exhibiting the target function.