Abstract: The invention provides an Escherichia coli for synthesizing L-valine, a construction method and use thereof. The Escherichia coli of the invention is designated as Escherichia coli W3110 and was deposited in China Center for Type Culture Collection (Address: Bayi Road, Wuchang District, Wuhan City, Hubei Province) under the Accession No. CCTCC M 2022293 on Mar. 18, 2022. The recombinant Escherichia coli takes Escherichia coli as a starting strain, and a transcription regulation factor is overexpressed to obtain a recombinant Escherichia coli. The recombinant Escherichia coli for synthesizing L-valine of the invention is fermented in a 5 L fermentor with trace dissolved oxygen to test strains, the yield of L-valine reaches 112 g/L, and the OD of the bacterium is 104.

Abstract: An enclosure, a battery and a power consuming device are provided. The enclosure is configured to accommodate battery cells and includes: a positioning member, including a bottom wall and a side wall connected to a circumference of the bottom wall, the side wall and the bottom wall enclosing and defining a positioning cavity, and the bottom wall being provided with a hollow portion; a fixing frame, located in the positioning cavity and configured to enclose the battery cells; and a backplate, disposed on one side of the bottom wall facing away from the positioning cavity. According to embodiments of the present application, the fixing frame can be rapidly assembled in the positioning cavity so that the assembly efficiency of the enclosure can be effectively improved.

Abstract: The present invention provides a multiplex PCR detection kit of Listeria monocytogenes serotype 4h. The kit includes a gene Imo1210 detection primer and a gene xysn_1693 detection primer. The present invention establishes a multiplex PCR method for rapidly detecting Listeria monocytogenes serotype 4h by using two pairs of primers.

Abstract: The present disclosure relates to a PCR detection kit for rapidly identifying Salmonella of specific serotypes. The kit includes primers for detecting tcpS gene, the primers for detecting the tcpS gene including a forward primer having a nucleotide sequence as set forth in SEQ ID NO. 1 and a reverse primer having a nucleotide sequence as set forth in SEQ ID NO. 2. The kit can identify Salmonella of enteritidis, pullorum/gallinarum, and dublin serotypes rapidly and in a high throughput, which can be used as an auxiliary method for the conventional serotyping of Salmonella.

Abstract: The present invention provides a multiplex PCR detection kit of Listeria monocytogenes serotype 4h. The kit includes a gene Imo1210 detection primer and a gene xysn_1693 detection primer. The present invention establishes a multiplex PCR method for rapidly detecting Listeria monocytogenes serotype 4h by using two pairs of primers.

Abstract: A communication method provided in embodiments of this application is a method for establishing an RRC connection between a terminal and a RAN, and the establishment of the RRC connection includes first RRC connection establishment and second RRC connection establishment. The first RRC connection establishment is a wireless RRC connection between the terminal and a first RAN network element, and the second RRC connection establishment is a wireless or wired RRC connection between the first RAN network element and a second RAN network element.

Abstract: A communication method provided in embodiments of this application is a method for establishing an RRC connection between a terminal and a RAN, and the establishment of the RRC connection includes first RRC connection establishment and second RRC connection establishment. The first RRC connection establishment is a wireless RRC connection between the terminal and a first RAN network element, and the second RRC connection establishment is a wireless or wired RRC connection between the first RAN network element and a second RAN network element.

Abstract: The present disclosure relates to a PCR detection kit for rapidly identifying Salmonella of specific serotypes. The kit includes primers for detecting tcpS gene, the primers for detecting the tcpS gene including a forward primer having a nucleotide sequence as set forth in SEQ ID NO. 1 and a reverse primer having a nucleotide sequence as set forth in SEQ ID NO. 2. The kit can identify Salmonella of enteritidis, pullorum/gallinarum, and dublin serotypes rapidly and in a high throughput, which can be used as an auxiliary method for the conventional serotyping of Salmonella.