Abstract: An imaging flow cytometer device includes a housing holding a multi-color illumination source configured for pulsed or continuous wave operation. A microfluidic channel is disposed in the housing and is fluidically coupled to a source of fluid containing objects that flow through the microfluidic channel. A color image sensor is disposed adjacent to the microfluidic channel and receives light from the illumination source that passes through the microfluidic channel. The image sensor captures image frames containing raw hologram images of the moving objects passing through the microfluidic channel. The image frames are subject to image processing to reconstruct phase and/or intensity images of the moving objects for each color. The reconstructed phase and/or intensity images are then input to a trained deep neural network that outputs a phase recovered image of the moving objects. The trained deep neural network may also be trained to classify object types.

Abstract: An imaging flow cytometer device includes a housing holding a multi-color illumination source configured for pulsed or continuous wave operation. A microfluidic channel is disposed in the housing and is fluidically coupled to a source of fluid containing objects that flow through the microfluidic channel. A color image sensor is disposed adjacent to the microfluidic channel and receives light from the illumination source that passes through the microfluidic channel. The image sensor captures image frames containing raw hologram images of the moving objects passing through the microfluidic channel. The image frames are subject to image processing to reconstruct phase and/or intensity images of the moving objects for each color. The reconstructed phase and/or intensity images are then input to a trained deep neural network that outputs a phase recovered image of the moving objects. The trained deep neural network may also be trained to classify object types.

Abstract: A scanning system for fluorescent imaging includes a sample holder configured to hold a sample therein, the sample holder defining a sample holding region. A scanner head spans the sample holding region and is movable relative to the sample holder. An array of light sources is disposed on an opposing side of the sample holder and is angled relative thereto. Respective controller are operably coupled to the scanner head and the array of light sources, wherein one controller selectively actuates a one or more rows of the array of light sources and another controller controls movement of the scanner head to capture fluorescent light emitted from within the sample holder in response to illumination from the actuated light sources. A filter designed to filter out scattered light from the sample may be interposed between the sample holder and the scanner head.

Abstract: A scanning system for fluorescent imaging includes a sample holder configured to hold a sample therein, the sample holder defining a sample holding region. A scanner head spans the sample holding region and is movable relative to the sample holder. An array of light sources is disposed on an opposing side of the sample holder and is angled relative thereto. Respective controller are operably coupled to the scanner head and the array of light sources, wherein one controller selectively actuates a one or more rows of the array of light sources and another controller controls movement of the scanner head to capture fluorescent light emitted from within the sample holder in response to illumination from the actuated light sources. A filter designed to filter out scattered light from the sample may be interposed between the sample holder and the scanner head.