Electrochemical test strip for reducing the effect of direct interference current
This invention describes an electrochemical sensor which is adapted to reduce the effects of interfering compounds in bodily fluids when measuring an analyte in such fluids using an electrochemical strip. The sensor includes a substrate, a first and second working electrodes, and a reference electrode. A reagent layer is disposed on the electrodes such that, in one embodiment it completely covers all of the first working electrode, but only partially covers the second working electrode and, in a second embodiment, it only covers a portion of the first and the second working electrode. The portion of the working electrodes not covered by the reagent layer and is used to correct for the interference effect on the analyte measurement.
The present invention claims priority to the following U.S. Provisional Applications: U.S. Provisional Application Ser. No. 60/516,252 filed on Oct. 31, 2003; U.S. Provisional Application Ser. No. 60/558,424 filed on Mar. 31, 2004; and U.S. Provisional Application Ser. No. 60/558,728 filed on Mar. 31, 2004. Which applications are hereby incorporated herein by reference.
RELATED APPLICATIONSThe present invention is related to the following co-pending U.S. applications: U.S. patent application Ser. No. ______ [Attorney Docket Number DDI-5027 USNP], filed on Oct. 29, 2004; U.S. patent application Ser. No. ______ [Attorney Docket Number DDI-5042 USNP], filed on Oct. 29, 2004; U.S. patent application Ser. No. ______ [Attorney Docket Number DDI-5065], filed on Oct. 29, 2004; U.S. patent application Ser. No. ______ [Attorney Docket Number DDI-5066], filed on Oct. 29, 2004; and U.S. patent application Ser. No. ______ [Attorney Docket Number DDI-5067], filed on Oct. 29, 2004.
FIELD OF THE INVENTIONThe present invention is related, in general to electrochemical strips and systems which are designed to reduce the effect of interfering compounds on measurements taken by such analyte measurement systems and, more particularly, to an improved electrochemical strip for reducing the effects of direct interference currents in a glucose monitoring system wherein the electrochemical strip has electrodes with uncoated regions.
BACKGROUND OF INVENTIONIn many cases, an electrochemical glucose measuring system may have an elevated oxidation current due to the oxidation of interfering compounds commonly found in physiological fluids such as, for example, acetaminophen, ascorbic acid, bilirubin, dopamine, gentisic acid, glutathione, levodopa, methyldopa, tolazimide, tolbutamide, and uric acid. The accuracy of glucose meters may, therefore, be improved by reducing or eliminating the portion of the oxidation current generated by interfering compounds. Ideally, there should be no oxidation current generated from any of the interfering compounds so that the entire oxidation current would depend only on the glucose concentration.
It is, therefore, desirable to improve the accuracy of electrochemical sensors in the presence of potentially interfering compounds such as, for example, ascorbate, urate, and, acetaminophen, commonly found in physiological fluids. Examples of analytes for such electrochemical sensors may include glucose, lactate, and fructosamine. Although glucose will be the main analyte discussed, it will be obvious to one skilled in the art that the invention set forth herein may also be used with other analytes.
Oxidation current may be generated in several ways. In particular, desirable oxidation current results from the interaction of the mediator with the analyte of interest (e.g., glucose) while undesirable oxidation current is generally comprised of interfering compounds being oxidized at the electrode surface and by interaction with the mediator. For example, some interfering compounds (e.g., acetominophen) are oxidized at the electrode surface. Other interfering compounds (e.g., ascorbic acid), are oxidized by chemical reaction with the mediator. This oxidation of the interfering compound in a glucose measuring system causes the measured oxidation current to be dependent on the concentration of both the glucose and any interfering compound. Therefore, in the situation where the concentration of interfering compound oxidizes as efficiently as glucose and the interferent concentration is high relative to the glucose concentration, the measurement of the glucose concentration would be improved by reducing or eliminating the contribution of the interfering compounds to the total oxidation current.
One known strategy that can be used to decrease the effects of interfering compounds is to use a negatively charged membrane to cover the working electrode. As an example, a sulfonated fluoropolymer such as NAFION™ may be used to repel all negatively charged chemicals. In general, most interfering compounds such as ascorbate and urate have a negative charge, thus, the negatively charged membrane prevents the negatively charged interfering compounds from reaching the electrode surface and being oxidized at that surface. However, this technique is not always successful since some interfering compounds such as acetaminophen do not have a net negative charge, and thus, can pass through a negatively charged membrane. Nor would this technique reduce the oxidation current resulting from the interaction of interfering compounds with some mediators. The use of a negatively charged membrane on the working electrode could also prevent some commonly used mediators, such as ferricyanide, from passing through the negatively charged membrane to exchange electrons with the electrode.
Another known strategy that can be used to decrease the effects of interfering compounds is to use a size selective membrane on top of the working electrode. As an example, a 100 Dalton exclusion membrane such as cellulose acetate may be used to cover the working electrode to exclude all chemicals with a molecular weight greater than 100 Daltons. In general, most interfering compounds have a molecular weight greater than 100 Daltons, and thus, are excluded from being oxidized at the electrode surface. However, such selective membranes typically make the test strip more complicated to manufacture and increase the test time because the oxidized glucose must diffuse through the selective membrane to get to the electrode.
Another strategy that can be used to decrease the effects of interfering compounds is to use a mediator with a low redox potential, for example, between about −300 mV and +100 mV (when measured with respect to a saturated calomel electrode). Because the mediator has a low redox potential, the voltage applied to the working electrode may also be relatively low which, in turn, decreases the rate at which interfering compounds are oxidized by the working electrode. Examples of mediators having a relatively low redox potential include osmium bipyridyl complexes, ferrocene derivatives, and quinone derivatives. A disadvantage of this strategy is that mediators having a relatively low potential are often difficult to synthesize, unstable and have a low water solubility.
Another known strategy that can be used to decrease the effects of interfering compounds is to use a dummy electrode which is coated with a mediator. In some instances the dummy electrode may also be coated with an inert protein or deactivated redox enzyme. The purpose of the dummy electrode is to oxidize the interfering compound at the electrode surface and/or to oxidize the mediator reduced by the interfering compound. In this strategy, the current measured at the dummy electrode is subtracted from the total oxidizing current measured at the working electrode to remove the interference effect. A disadvantage of this strategy is that it requires that the test strip include an additional electrode and electrical connection (i.e., the dummy electrode) which cannot be used to measure glucose. The inclusion of dummy electrode is an inefficient use of an electrode in a glucose measuring system.
SUMMARY OF INVENTIONThe invention described herein is directed to an electrochemical sensor which reduces the effects of interferences. An electrochemical sensor according to the present invention includes a substrate, at least first and second working electrodes and a reference electrode. In one embodiment of an electrochemical sensor according to the present invention, a reagent layer is disposed on the electrodes such that it completely covers all of the first working electrode and only partially covers the second working electrode. In a method according to the present invention, the oxidation current generated at the portion of the second working electrode not covered by the reagent layer is used to correct for the effect of interfering substances on the glucose measurement.
In one embodiment of the present invention, the electrochemical glucose test strip includes a first and second working electrodes, where the first working electrode is completely covered with a reagent layer and the second working electrode is only partially covered with the reagent layer. Thus, the second working electrode has a reagent coated area and an uncoated area. The reagent layer may include, for example, a redox enzyme such as glucose oxidase and a mediator such as, for example, ferricyanide. The first working electrode will have a superposition of two oxidation current sources, one from glucose and a second from interferents. Similarly, the second working electrode will have a superposition of three oxidation current sources from glucose, interferents at the reagent coated portion, and interferents at the uncoated portion. The uncoated portion of the second working electrode will only oxidize interferents and not oxidize glucose because there is no reagent is in this area. The oxidation current measured at the uncoated portion of the second working electrode may then be used to estimate the total interferent oxidation current and calculate a corrected oxidation current which removes the effects of interferences.
In an alternative strip embodiment according to the present invention, the electrochemical glucose test strip includes a first and second working electrodes, where the first and second working electrode are only partially covered with the reagent layer. Thus, in this embodiment both the first and second working electrode have a reagent coated portion and an uncoated portion. The first uncovered area of the first working electrode and the second uncovered area of the second working electrode are different. The oxidation current measured at the uncoated portion of the first and second working electrodes are used to estimate the interferent oxidation current for the uncoated portion and to calculate a corrected glucose current.
BRIEF DESCRIPTION OF DRAWINGSA better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings, of which:
This invention described herein includes a test strip and method for improving the selectivity of an electrochemical glucose measuring system.
In one embodiment of the present invention, substrate 50 is an electrically insulating material such as plastic, glass, ceramic, and the like. In a preferred embodiment of this invention, substrate 50 may be a plastic such as, for example, nylon, polycarbonate, polyimide, polyvinylchloride, polyethylene, polypropylene, PETG, or polyester. More particularly the polyester may be, for example Melinex® ST328 which is manufactured by DuPont Teijin Films. Substrate 50 may also include an acrylic coating which is applied to one or both sides to improve ink adhesion.
The first layer deposited on substrate 50 is conductive layer 64 which includes first working electrode 10, second working electrode 12, reference electrode 14, and strip detection bar 17. In accordance with the present invention, a screen mesh with an emulsion pattern may be used to deposit a material such as, for example, a conductive carbon ink in a defined geometry as illustrated in
First contact 11, second contact 13, and reference contact 15 may be used to electrically interface with a meter. This allows the meter to electrically communicate to first working electrode 10, second working electrode 12, and reference electrode 14 via, respective, first contact 11, second contact 13, and reference contact 15.
The second layer deposited on substrate 50 is insulation layer 16. Insulation layer 16 is disposed on at least a portion of conductive layer 64 as shown in
The third layer deposited on substrate 50 is a reagent layer 22. Reagent layer 22 is disposed on at least a portion of conductive layer 64 and insulation layer 16 as shown in
The fourth layer deposited on substrate 50 is an adhesive layer 66 which includes a first adhesive pad 24, a second adhesive pad 26, and a third adhesive pad 28. First adhesive pad 24 and second adhesive pad 26 form the walls of a sample receiving chamber. In one embodiment of the present invention, first adhesive pad 24 and second adhesive pad 26 may be disposed on substrate 50 such that neither of the adhesive pads touches reagent layer 22. In another embodiments of the present invention where the strip volume needs to be reduced, first adhesive pad 24 and/or second adhesive pad 26 may be disposed on substrate 50 such there is overlap with reagent layer 22. In an embodiment of the present invention, adhesive layer 66 has a height of about 70 to 110 microns. Adhesive layer 66 may include a double sided pressure sensitive adhesive, a UV cured adhesive, heat activated adhesive, thermosetting plastic, or other adhesive known to those skilled in the art. As a non-limiting example, adhesive layer 66 may be formed by screen printing a pressure sensitive adhesive such as, for example, a water based acrylic copolymer pressure sensitive adhesive which is commercially available from Tape Specialties LTD in Tring, Herts, United Kingdom (part#A6435).
The fifth layer deposited on substrate 50 is a hydrophilic layer 68 which includes a first hydrophilic film 32 and second hydrophilic film 34 as illustrated in
The sixth and final layer deposited on substrate 50 is a top layer 40 which includes a clear portion 36 and opaque portion 38 as illustrated in
The first test strip embodiment as illustrated in
A further embodiment of the present invention as illustrated in
For the strip embodiment illustrated in
The second layer deposited on substrate 50 in
The third layer deposited on substrate 50 in
For the strip embodiment illustrated in
In accordance with the present invention, distal cutout width W11, proximal cutout width W12, distal cutout length L14 and proximal cutout length L15 may have a respective dimension of approximately 1.1, 0.7, 2.5, and 2.6 mm.
In the embodiment of
In the embodiment of the invention illustrated in
An algorithm may, therefore be used to calculate a corrected glucose current that is independent of interferences. After dosing a sample onto a test strip, a constant potential is applied to the first and second working electrodes and a current is measured for both electrodes. At the first working electrode where reagent covers the entire electrode area, the following equation can be used to describe the components contributing to the oxidation current,
WE1=G+Icov (Eq 1)
where WE1 is a current density at the first working electrode, G is a current density due to glucose which is independent of interferences, and Icov is a current density due to interferences at the portion of a working electrode covered with reagent.
At the second working electrode which is partially covered with reagent, the following equation can be used to describe the components contributing to the oxidation current,
WE2=G+Icov+Iunc (Eq 2)
where WE2 is a current density at the second working electrode and Iunc is a current density due to interferences at the portion of a working electrode not covered with reagent.
To reduce the effects of interferences, an equation is formulated which describes the relationship between the interferent current at the coated portion of the second working electrode and the uncoated portion of the second working electrode. It is approximated that the interferent oxidation current density measured at the coated portion is the same as the current density measured at the uncoated portion. This relationship is further described by the following equation,
where Acov is an area of second working electrode covered with reagent and Aunc is an area of second working electrode not covered with reagent.
Uncoated portions 12u can oxidize interferents, but not glucose because it is not coated with reagent layer 22. In contrast, coated portion 12c can oxidize glucose and interferents. Because it was experimentally found that uncoated portions 12u oxidizes interferents in a manner proportional to the area of coated portion 12c, it is possible to predict the proportion of interferent current measured overall at second working electrode 12. This allows the overall current measured at second working electrode 12 to be corrected by subtracting the contribution of the interferent current. In an embodiment of the present invention the ratio of Aunc:Acov may be between about 0.5:1 to 5:1, and is preferably about 3:1. More details describing this mathematical algorithm for current correction will be described in a later section.
In an alternative embodiment of the present invention, the interferent oxidation current density measured at the coated portion may be different than the current density measured at the uncoated portion. This may be ascribed to a more efficient or less efficient oxidation of interferents at the coated portion. In one scenario, the presence of a mediators may enhance the oxidation of interferences relative to the uncoated portion. In another scenario, the presence of viscosity increasing substances such as hydroxyethyl cellulose may decrease the oxidation of interferences relative to the uncoated portion. Depending on the components included in the reagent layer which partially coats the second working electrode, it is possible that the interferent oxidation current density measured at the coated portion may be more or less than the uncoated portion. This behavior may be phenomenologically modeled by re-writing Equation 3a to the following form,
Icov=f×Iunc (Eq 3b)
where f is a correction factor which incorporates the effects of the interferent oxidation efficiency of the coated to uncoated portion.
In an embodiment of the present invention, Equation 1, 2, and 3a may be manipulated to derive an equation that outputs a corrected glucose current density independent of interferences. It should be noted that the three equations (Equation 1, 2, and 3a) collectively have 3 unknowns which are G, Icov, and Iunc. Equation 1 can be rearranged to the following form.
G=WE1−Icov (Eq 4)
Next, Icov from Equation 3a can be substituted into Equation 4 to yield Equation 5.
Next, Equation 1 and Equation 2 can be combined to yield Equation 6.
Iunc=WE2−WE1 (Eq 6)
Next, Iunc from Equation 6 can be substituted into Equation 5 to yield Equation 7a.
Equation 7a outputs a corrected glucose current density G which removes the effects of interferences requiring only the current density output of the first and second working electrode, and a proportion of the coated to uncoated area of the second working electrode. In one embodiment of the present invention the proportion
may be programmed into a glucose meter, in, for example, a read only memory. In another embodiment of the present invention, the proportion
may be transferred to the meter via a calibration code chip which would may account for manufacturing variations in Acov or Aunc.
In an alternative embodiment to the present invention Equation 1, 2, and 3b may be used when the interferent oxidation current density for the coated portion is different from the interferent oxidation current density of the uncoated portion. In such a case, an alternative correction Equation 7b is derived as shown below.
G=WE1−{f×(WE2×WE1)} (Eq 7b)
In another embodiment of the present invention, the corrected glucose current Equation 7a or 7b may be used by the meter only when a certain threshold is exceeded. For example, if WE2 is about 10% or greater than WE1, then the meter would use Equation 7a or 7b to correct for the current output. However, if WE2 is about 10% or less than WE1, the meter would simple take an average current value between WE1 and WE2 to improve the accuracy and precision of the measurement. The strategy of using Equation 7a or 7b only under certain situations where it is likely that a significant level of interferences are in the sample mitigates the risk of overcorrecting the measured glucose current. It should be noted that when WE2 is sufficiently greater than WE1 (e.g. about 20% or more), this is an indicator of having a sufficiently high concentration of interferents. In such a case, it may be desirable to output an error message instead of a glucose value because a very high level of interferents may cause a breakdown in the accuracy of Equation 7a or 7b.
In the embodiment of the present invention illustrated in
Test strips 2000 and 5000 have an advantage in that they may be easier to manufacture in regards to depositing the reagent layer with the required registration and also any subsequently deposited layers. Furthermore, both the first and second working electrodes will have to some extent the same chemical and electrochemical interactions with any interfering substances thus ensuring greater accuracy in the correction process. With both working electrodes having some level of uncoated area the same reactions will occur on both electrodes but to a different extent. Using a simple modification to Equation 7a, the following Equation 7c can be used as the correction equation for glucose,
where
Aunc1=is an uncoated area of the first working electrode, Aunc2=is an uncoated area of the second working electrode, Acov1=is a coated area of the first working electrode, and Acov2=is a coated area of the second working electrode.
One advantage of the present invention is the ability to use the first and second working electrode to determine that the sample receiving chamber has been sufficiently filled with liquid. It is an advantage of this invention in that the second working electrode not only corrects the interferent effect, but can also measure. glucose. This allows for a more accurate results because 2 glucose measurements can be averaged together while using only one test strip.
EXAMPLE 1 Test strips were prepared according to the first embodiment of the present invention as illustrated in
To show that the method of correcting the current for interferents applies to a wide variety of interferents, strips built according to the embodiment of
In test strip 800, conductive layer 802 is the first layer disposed on substrate 50. Conductive layer 802 includes a second working electrode 806, a first working electrode 808, a reference electrode 810, a second contact 812, a first contact 814, a reference contact 816, a strip detection bar 17, as shown in
Insulation layer 804 is the second layer disposed on substrate 50. Insulation layer 16 includes a cutout 18 which may have a rectangular shaped structure. Cutout 18 exposes a portion of second working electrode 806, first working electrode 808, and reference electrode 810 which can be wetted with a liquid. The material used for insulation layer 804 and the process for printing insulation layer 804 is the same for both test strip 62 and test strip 800.
Reagent layer 820 is the third layer disposed on substrate 50, first working electrode 808 and reference electrode 810. The material used for reagent layer 820 and the process for printing reagent layer 820 is the same for both test strip 62 and test strip 800.
Adhesive layer 830 is the fourth layer disposed on substrate 50. The material used for adhesive layer 830 and the process for printing adhesive layer 830 is the same for both test strip 62 and test strip 800. The purpose of adhesive layer 830 is to secure top layer 824 to test strip 800. In an embodiment of this invention, top layer 824 may be in the form of an integrated lance as shown in
Lance 826, which may also be referred to as a penetration member, may be adapted to pierce a user's skin and draw blood into test strip 800 such that second working electrode 806, first working electrode 808, and reference electrode 810 are wetted. Lance 826 includes a lancet base 832 that terminates at distal end 58 of the assembled test strip. Lance 826 may be made with either an insulating material such as plastic, glass, and silicon, or a conducting material such as stainless steel and gold. Further descriptions of integrated medical devices that use an integrated lance can be found in International Application No. PCT/GB01/05634 and U.S. patent application Ser. No. 10/143,399. In addition, lance 826 can be fabricated, for example, by a progressive die-stamping technique, as disclosed in the aforementioned International Application No. PCT/GBO1/05634 and U.S. patent application Ser. No. 10/143,399.
When performing a test, first voltage source 910 applies a first potential E1 between the second working electrode and the reference electrode; and second voltage source 920 applies a second potential E2 between the first working electrode and the reference electrode. In one embodiment of this invention, first potential E1 and second potential E2 may be the same such as for example about +0.4 V. In another embodiment of this invention, first potential El and second potential E2 may be different. A sample of blood is applied such that the second working electrode, the first working electrode, and the reference electrode are covered with blood. This allows the second working electrode and the first working electrode to measure a current which is proportional to glucose and/or non-enzyme specific sources. After about 5 seconds from the sample application, meter 900 measures an oxidation current for both the second working electrode and the first working electrode.
It will be recognized that equivalent structures may be substituted for the structures illustrated and described herein and that the described embodiment of the invention is not the only structure which may be employed to implement the claimed invention. In addition, it should be understood that every structure described above has a function and such structure can be referred to as a means for performing that function. While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to hose skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.
Claims
1. An electrochemical sensor comprising:
- a substrate;
- a first working electrode disposed on said substrate;
- a second working electrode disposed on said substrate;
- a reference electrode; and
- a reagent layer disposed on said first working electrode, wherein said reagent layer completely covers said first working electrode;
- said second working electrode including a covered portion and an uncovered portion wherein said covered portion of said second working electrode is covered by said reagent layer.
2. An electrochemical sensor according to claim 1 wherein:
- said first working electrode, said second working electrode and said reference electrode are positioned in a sample receiving chamber;
- said sample receiving chamber having a proximal and a distal end, said distal end including a first opening which is adapted to receive bodily fluids; and
- said uncovered portion of said second working electrode is positioned adjacent said first opening.
3. An electrochemical sensor according to claim 2 wherein said covered portion of said second working electrode is positioned at a proximal end of said sample receiving chamber.
4. An electrochemical sensor according to claim 3 wherein said first working electrode is positioned proximal to said uncovered portion of said second working electrode and between said reference electrode and said covered portion of said second working electrode.
5. An electrochemical sensor according to claim 1 wherein:
- said first working electrode, said second working electrode and said reference electrode are positioned in a sample receiving chamber;
- said sample receiving chamber having a proximal and a distal end, said distal end including a first opening which is adapted to receive bodily fluids; and
- said uncovered portion of said second working electrode comprising two sections, wherein each said section is positioned adjacent said covered portion of said second working electrode.
6. An electrochemical sensor according to claim 5, wherein:
- said first working electrode is positioned adjacent said distal end of said sample receiving chamber;
- said second working electrode is positioned adjacent said proximal end of said sample receiving chamber; and
- said reference electrode is positioned between said first and said second working electrodes.
7. An electrochemical sensor comprising:
- a substrate;
- a first working electrode disposed on said substrate;
- a second working electrode disposed on said substrate;
- a reference electrode; and
- a reagent layer disposed on a portion said first working electrode and said second working electrode;
- said first working electrode having a reagent coated area and an uncoated area; and
- said second working electrode having a reagent coated area and an uncoated area.
8. An electrochemical sensor according to claim 7 wherein:
- said first working electrode, said second working electrode and said reference electrode are positioned in a sample receiving chamber;
- said sample receiving chamber has a proximal and a distal end, said distal end including a first opening which is adapted to receive bodily fluids; and
- said uncovered portion of said first working electrode comprises two sections, wherein each said section is positioned adjacent said covered portion of said first working electrode; and
- said uncovered portion of said second working electrode comprises two sections, wherein each said section is positioned adjacent said covered portion of said first working electrode.
9. An electrochemical sensor according to claim 8, wherein:
- said first working electrode is positioned adjacent said distal end of said sample receiving chamber;
- said second working electrode is positioned adjacent said proximal end of said sample receiving chamber; and
- said reference electrode is positioned between said first and said second working electrodes.
10. An electrochemical sensor according to claim 7 wherein said uncoated area of said first working electrode is not equal to said uncoated area of said second working electrode.
11. An electrochemical sensor according to claim 7 wherein:
- said first working electrode, said second working electrode and said reference electrode are positioned in a sample receiving chamber;
- said sample receiving chamber has a proximal and a distal end, said distal end including a first opening which is adapted to receive bodily fluids;
- said uncovered portion of said second working electrode is positioned at a proximal end of said sample receiving chamber; and
- said uncovered portion of said first working electrode is positioned proximal to said uncovered portion of said second working electrode.
12. An electrochemical sensor according to claim 11, wherein:
- said covered portion of said first working electrode is positioned proximal to said uncovered portion of said first working electrode; and
- said covered portion of said second working electrode is positioned proximal to said covered portion of said first working electrode.
13. An electrochemical sensor according to claim 1, further including an integrated lance at a distal end of said lance.
14. An electrochemical sensor according to claim 7, further including an integrated lance at a distal end of said electrochemical sensor.
Type: Application
Filed: Oct 29, 2004
Publication Date: Jun 23, 2005
Inventors: Oliver Davies (Croy), Robert Marshall (Conon Bridge), Damian Baskeyfield (Auldeam), Lynsey Whyte (Lochardil), Elaine Leiper (Dores Road)
Application Number: 10/976,489