Process for enzymatic deinking of printed papers
The present invention provides a process for enzymatic deinking of office waste paper inclusive of xerographic and inkjet-printed paper printed with non impact and non dispersible ink using the cell free culture supernatant of Vibrio alginolyticus or immobilized cells of the same bacterium having the accession number NIO/DI/32 and deposited at ARS Patent Culture Collection, USA, under the Number NRRL-B-30638. Further, this invention relates to decolorization of office waste paper pulp within 72 h after contacting the said enzymes from the said bacterium in the pulp of 3-12% consistency in seawater. Decolorization is achieved by using extracellular enzymes produced by the bacterium NIO/DI/32 grown in nutrient broth supplemented with starch or Tween 80.
The present invention relates to process for enzymatic deinking of office waste paper inclusive of xerographic and inkjet-printed paper printed with non impact and non dispersible ink.
More particularly, it relates to a process for enzymatic deinking of office waste paper inclusive of xerographic and inkjet-printed paper printed with non impact and non dispersible ink using the cell free culture supernatant of Vibrio alginolyticus or immobilized cells of the same bacterium having the accession number NIO/DI/32 and deposited at ARS Patent Culture Collection,USA, under the Number NRRL-B-30638.
BACKGROUND OF THE INVENTIONPaper manufacturing is a major industry and a continuously growing one. Increased production of paper imposes a severe demand on plant raw material and thus harms the environment. Recycling of used paper is an alternative that can alleviate the stress that is exercised on the environment. The three major sources of raw material for such recycling are newsprint, photocopied paper and inkjet—printed papers. Recycling of paper requires the removal of the printing ink from the used paper, called deinking, so that the processed material is brighter. Printing on paper is accomplished by using two types of inks, the impact and the non-impact inks. In impact inks, used for newsprints, the ink does not fuse with the paper and is, therefore, easy to remove or disperse during the deinking, or recycling process. Such recycling is now well known and has been carried out for years. On the contrary, non-impact inks used in photocopying, ink-jet printing and laser printing results in the ink fusing with the paper and makes it non-dispersible, thus rendering the deinking process much more difficult (Jeffries, 1996, website “Bioenergy”). The toners used for photocopying generally contain iron oxide (about 55%), olefins (about 5%) and plastic or polymer (Website:http://www.eng-tips.com).
Various hydrolytic enzymes such as cellulase, lipase, amylase of bacterial or fungal origin, individually or in combination have been used for deinking of office waste paper.
(i) A reference may be made to a publication wherein, a method for deinking of xerographic and printed paper, collectively called office waste, includes applying deinking solution to the paper to be deinked, the deinking solution being composed of a cleaning solution and a surfactant, abrading the paper to remove the ink from paper, and washing the paper to remove the deinking solution from the paper (U.S. Pat. No. 6,022,423 dtd Feb. 8, 2000). However, this patent involves using a special device for deinking of whole sheets and cannot be used on pulp.
(ii) A reference may be made to a publication wherein, a mono component cellulase is used for removal of ink and toners from printed paper (U.S. Pat. No. 5,525,193). The technique of isolating mono component cellulase from microorganisms by various purifications, chromatographic steps which is time consuming and expensive.
(iii) A reference may be made to a publication wherein, endogluconases and hemicellulases from several fungi are used effectively for deinking of xerox and laser-printed paper (Gubitz et al., 1998). However, the pulp needs to be treated with surfactants and washed to remove the floating ink particles.
(iv) A reference may be made to a publication, wherein deinking of laser printed or xerographic paper is done using enzyme containing a resistant binder. Enzyme is added in the specified form to improve speck removal (Patent No. GB 2304741 dated Mar. 26, 1997). As with the above references, the technology to remove the ink by floatation remains a problem.
(v) A reference may be made to a publication, wherein deinking of office waste paper is carried out by contacting the pulp with a deinking agent containing enzymes cellulase, preferably active at pH 4 to 8. The treated paper is used in the production of newsprint and high quality paper (Patent No. EP 717144 dated Jun. 19, 1996). As with the above references, the technology to remove the ink by floatation remains a problem.
(vi) A reference may be made to a publication, wherein deinking of waste paper is achieved by incubating the paper pulp with cellulase at 40° C. for 1 hour under pressure. Following dissociation of ink particles, the resulting washed pulp is brighter (Patent No. JP 06346390 dated Dec. 20, 1994). The technology for incubation under pressure and high temperature is an expensive process.
(vii) A reference may be made to a publication, wherein deinking is achieved by using lipase to remove ink from used paper. The procedure is carried out under neutral or alkaline conditions which produces regenerated pulp having enhanced brightness (Patent No. JP 2160984 dated Jun. 20, 1990). The ink particles are removed by washing and floatation.
(viii) A reference may be made to a publication, wherein deinking of waste paper is done by using the yeast Hansenula polymorpha, which degrades the ink-based resin (Patent No. KR 9303705 dated may 8, 1993). However, the ink floats on the surface and the pulp has to be extensively washed to remove the detached ink.
(ix) A reference may be made to a publication, wherein decolorization of Indian ink is achieved by using thermostable alkaline cellulase from a Bacilus sp (Patent number JP10313859). However, this process is very specific to Indian ink.
(x) A reference may be made to a patent filed, wherein decolorization of office waste is achieved by inoculating the paper pulp with the bacterium Vibrio alginolyticum (US Patent application Number 20030178162). However, handling the live bacterium is a big disadvantage in this process and may not be acceptable to many.
OBJECTS OF THE INVENTIONThe main object of the present invention is to provide a process for enzymatic deinking of office waste paper inclusive of xerographic and inkjet-printed paper printed with non impact and non dispersible ink.
Another object of the present invention is to provide a process for enzymatic deinking of office waste paper inclusive of xerographic and inkjet-printed paper printed with non impact and non dispersible ink using the cell free culture supernatant of Vibrio alginolyticus or immobilized cells of the same bacterium having the accession number NIO/DI/32 and deposited at ARS Patent Culture Collection, USA, under the Number NRRL-B-30638.
SUMMARY OF THE INVENTIONThe present invention relates to a process for enzymatic deinking of office waste paper inclusive of xerographic and inkjet-printed paper printed with non impact and non dispersible ink using the cell free culture supernatant of Vibrio alginolyticus or immobilized cells of the same bacterium having the accession number NIO/DI/32 and deposited at ARS Patent Culture Collection, USA, under the Number NRRL-B-30638. Further, this invention also relates to decolorization of office waste paper pulp within 72 h after contacting the said enzymes from the said bacterium in the pulp of 3-12% consistency in seawater. Decolorization is achieved by using extracellular enzymes produced by the bacterium NIO/DI/32 grown in nutrient broth supplemented with starch or Tween 80.
DETAILED DESCRIPTION OF THE INVENTIONDeinking of paper is a limiting factor in recycling of paper. Newsprint and office waste paper is generally deinked using chemicals which go into the effluent and cause pollution of the water bodies. The newsprint or offset printing is done using dispersible or non-impact ink whereas laser, xerographic and ink-jet printer ink does not disperse and is also called impact ink. There are several patents describing various methods of deinking by using hydrolytic enzymes such as cellulase, hemicellulase and lipase of microbial origin which release toner particles from fibers. However, enzyme action is affected by the paper constituents in the deinking condition. The chemically pulped fibers are more susceptible than mechanically pulped fibers. Office waste paper is high in laser and toner content and the technology for taking the toner particles out is not very good at the moment (Jefferies, 2002). We report here a process for deinking of office waste paper comprising xerographic and ink-jet printed paper by cell free culture filtrate of a bacterium isolated from marine sediment of an estuary in Goa, India. The salinity of the water at the time of isolation was around 15 parts per thousand which is roughly equivalent to half-strength sea water.
The organism given in the present invention is Vibrio alginolyticus, a'gram-negative, coccoid bacterium isolated from coastal marine sediment and given the accession number NIO/DI/32 and is deposited in ARS patent culture collection center having accession number NRRL-B-30638. The said bacterium is grown in nutrient broth containing beef extract, peptic digest of animal tissue, prepared with sea water with salinity diluted to 50% and having a pH of at least 7.5 and supplemented with 1% starch or 1% Tween-80 solution or both. The bacterium when grown in nutrient agar medium appears as circular colonies off-white in color, having 2 mm diameter in the beginning and growing to a size of 5 mm within 3-4 days. The bacterium produces esterase/lipase and amylase in the presence of Tween-80 and starch respectively. It is fermentative and catalase-negative bacterium. The bacterium is grown at room temperature (30° C.) for about 4 days, the culture supernatant is collected by centrifugation under sterile conditions. Xerographic, ink-jet printed paper and other papers printed with impact ink are pulped by soaking in hot water for a minimum of 2 hrs, macerated in a domestic mixer with surfactant such as Tween-80. An example for the process for deinking involves suspending such a pulp at least at 3-12% consistency in seawater with salinity diluted to 50%, incubating with cell-free culture supernatant of the said bacterium. The treated pulp is incubated at room temperature or 37° C. for a minimum of 2-3 days to get completely decolorized pulp without traces of ink in the supernatant. The pulp is diluted, aerated from bottom to remove free contaminating particles and salts. Soap solution is added at 1% concentration for flotation of any residual ink particles. The pulp is filtered over a Buchner funnel under suction, pressed with flat stainless steel plates to make the pulp sheet uniform in thickness. The radiance of the resultant sheet is read from 412 to 684 nm and the radiance expressed in Lux units (Lu). One LU is equivalent to W/cm2/nm/Sr. The ratio of radiance between enzymatically deinked and recycled paper purchased from the market (hereafter called the reference paper) is taken as a measure of brightness.
Most of the methods used for enzymatic deinking release ink particles from the fibers and they need to be subsequently washed off from the pulp. In the process described in the present invention, this problem does not arise as the ink and toner particles are totally decolorized. The method is very cost effective as the only step involved is raising the bacterial inoculum in any conventional nutrient broth containing assimilable carbon and nitrogen source and supplemented with starch or Tween-80. A comparative statement given in Table 1 explains the novelty of our. invention in comparison with prior art known in this field.
Accordingly the present invention provides a process for enzymatic deinking of printed papers inclusive of xerographic and inkjet-printed paper printed with non impact and non dispersible ink comprising:
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- a) growing the bacterial isolate Vibrio alginolyticus having accession number NIO/DI/32, the culture being deposited at ARS Patent Culture Collection under NRRL Number NRRL-B-30638, in a novel medium at a temperature ranging from 27° C. to 37° C. for 3-4 days;
- b) removing the bacterial cells by centrifugation under sterile condition to obtain cell free culture supernatant;
- c) incubating the xerox or inkjet-printed paper pulp diluted to 3-12% consistency in sea water with salinity diluted to 50% with the resulting cell free culture supernatant obtained from step (b) at 20% concentration for at least 72 hours at room temp followed by diluting the pulp ten fold in water to obtain completely decolorized pulp without traces of ink in the supernatant;
- d) filtering the resulting pulp obtained from step (c) under suction to get the desired deinked paper.
In an embodiment of the present invention, the medium is prepared by dissolving nutrient broth contains beef extract, peptic digest of animal tissue in sea water with salinity diluted to 50% and supplemented with tween 60 or 1% starch or both.
In another embodiment of the present invention, the bacterium is grown for a minimum of 4 days in nutrient broth containing beef extract, peptic digest of animal tissue in sea water with salinity diluted to 50% and supplemented with tween 60 or 1% starch or both.
In yet another embodiment of the present invention, the sodium chloride at a minimum concentration of 1.5% can be used in the place of sea water for growing the bacterium.
In yet another embodiment of the present invention, cell free supernatant contains the extracellular enzymes such as esterase, lipase and amylase etc, used for deinking of papers.
In still another embodiment of the present invention, the xerographic or inkjet-printed paper is soaked in hot water for 1-2 hours after addition of 1% surfactant followed by macerating it to pulp using any domestic mixer.
In still another embodiment of the present invention, the pulp sheet is pressed to make it uniform and dried at about 60° C. for a minimum of 4-5 hours.
In still another embodiment of the present invention, the radiance of the resultant sheet is read from 412 to 684 nm and the radiance expressed as Lux units (LU). One LU is equivalent to μW/cm2/nm/Sr.
In still another embodiment of the present. invention, the pulp sheet made out of recycled paper available in the market is used as a reference.
In still another embodiment of the present invention, the same batch of cell free culture supernatant can be effectively used for two-three cycles of deinking of xerox paper/inkjet-printed paper pulp.
In still another embodiment of the present invention, the lyophilized culture supernatant can also be effectively used for deinking of the said pulp.
In still another embodiment of the present invention, bacteria immobilized in sodium alginate beads can also be used for deinking the xerox paper/inkjet printed paper pulp to get completely bleached pulp within 48-72 hours.
The following examples are given by way of illustration of the present invention and should not be construed to limit the scope of present invention.
EXAMPLE 1The bacterial isolate Vibrio alginolyticus NIO/DI/32 was isolated from the coastal esturine sediment, Dona Paula, Goa, India in nutrient agar medium containing beef extract and peptic digest of animal tissue and agar in sea water with its salinity diluted to 50%. The culture was maintained in the slants of nutrient agar medium for all further experiments.
The said bacterium is grown in nutrient broth containing beef extract, peptic digest of animal tissue in sea water with its salinity diluted to 50%. Besides, the medium is amended either with starch or Tween-80 or a both together used at 1% concentration. The bacterium is grown at room temperature (30° C.) for about 4 days, the bacterial cells are removed by centrifugation and the cell-free culture supernatant is used for deinking purposes. Xerographic paper printed with impact ink from HP or Epson or any other printer are pulped by soaking in hot water for a minimum of 2 hrs, macerated in a conventional domestic mixer with surfactant such as Tween 80. An example for the process for deinking involves suspending such a pulp at 3, 6 and 9% consistency (g wet pulp in 100 ml of water) incubating with 50 ml of cell free culture supernatant prepared as described above and incubating at room temperature for a minimum of 3 days to get completely bleached pulp with the ink totally disappearing from the supernatant water. The pulp is diluted, aerated from bottom to remove free contaminating particles. Soap solution is added at 1% concentration for flotation of any residual ink particles. The pulp is filtered over a Buchner funnel under suction, pressed with flat stainless steel plates to make the pulp sheet uniform in thickness. The radiance of the resultant sheet is read from 412 to 684 nm and the radiance expressed in Lux units (Lu). One LU is equivalent to μW/cm2 /nM/Sr. The ratio of reflectance between recycled paper available in any stationery shop (reference paper) and biologically deinked paper is taken as a measure of brightness. The reflectance of untreated pulp is the negative control.
Accordingly,
The aim of the present experiment was to compare deinking of pulp using the bacterial culture supernatants containing starch and Tween 80 at room temperature and at 37° C.
The printed pulp at 3% consistency was incubated with bacterial culture supernatant containing starch or Tween 80 for 72 hours at 37° C. At the end of the incubation period, the slurry was filtered, washed and dried as described in the Example 1. The reflectance was measured as detailed above.
Accordingly,
In order to avoid direct contact with bacterial inoculum, the ability of the immobilized bacterial isolate NIO/DI/32 to deink inkjet printed paper was carried out as follows:
Bacterial cells were immobilized in sodium alginate. Briefly, this involved mixing 8 ml of cells (containing 108 cell ml−1) with 25 ml of 2% alginic acid (Sigma Chemicals, USA). The beads were prepared in 0.2 M calcium chloride solution and were cured for overnight. The same batch of immobilized bacterial cells were used in two cycles to achieve deinking. The other procedures used were the same as described in the Example 1.
Accordingly,
The deinking of inkjet-printed paper was carried out using heat-killed bacterial cells and heat-inactivated culture supernatants (containing starch or Tween 80). For this purpose the bacterial cells and the culture supernatants were autoclaved at 121° C. for 15 minutes at 15 lb.
The preparation of pulp, inoculum and experimental set up and incubation were same as in the previous examples.
Accordingly,
The effect of storage of culture supernatant is one of the important criteria for its effective use. Therefore, the crude culture supernatant stored for 2 days at room temperature was used for deinking of the pulp. For comparison, the fresh culture filtrate was used for deinking the pulp. The procedure for deinking was the same as described in examples 1-4.
Accordingly,
We also compared the efficiency of the freeze-dried culture filtrate on deinking. Accordingly, Table 4 shows that the freeze-dried culture filtrate from medium containing starch or Tween 80 could effectively deink the pulp.
The freeze-dried culture filtrate (CF) was thawed and diluted to the original volume with 50% diluted sea water and incubated with pulp. The negative control was incubated with 50% diluted sea water.
EXAMPLE 6The active principles involved in deinking of the pulp using culture supernatant of media containing starch or Tween 80 were investigated. Accordingly, amylase and lipase activities were measured in these supernatants. Amylase activity was measured using 1% starch, iodine and potassium iodide (Medda and Chandra 1980). Lipase activity was measured using model substrate 4-methylumbelliferyle-butyrate (Sigma Chemicals, USA). The fluorescent MUF product released is measured at excitation and emission wave length of 364 and 445 nm respectively (Hoppe H-G. 1993). The use of Tween 80 in the medium will induce esterase as well as lipase activity (Gupta et al. 2003).
Accordingly the 5-day old culture filtrate containing starch and Tween 80 showed 49 and 33 U ml−1 activity respectively. These were the minimum concentrations required for effective deinking because diluting the enzyme solutions did not result in effective deinking.
The main advantages of the present invention are:
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- 1. Cell free supernatant can be reused for deinkinking of office waste paper inclusive of xerographic and inkjet-printed paper printed with non impact and non dispersible ink.
- 2. There is no need of enzyme purification in the present process.
- 3. No temperature and pH adjustment is required.
Claims
1. A process for enzymatic deinking of printed papers inclusive of xerographic and inkjet- printed paper printed with non impact and non dispersible ink comprising:
- a) growing the bacterial isolate Vibrio alginolyticus having accession number NIO/DI/32, the culture being deposited at ARS Patent Culture Collection under NRRL Number NRRL-B-30638, in a novel medium at a temperature ranging from 27° C. to 37° C. for 3-4 days;
- b) removing the bacterial cells by centrifugation under sterile condition to obtain cell free culture supernatant;
- c) incubating the xerox or inkjet-printed paper pulp diluted to 3-12% consistency in sea water with salinity diluted to 50% with the resulting cell free culture supernatant obtained from step (b) at 20% concentration for at least 72 hours at room temp followed by diluting the pulp ten fold in water to obtain completely decolorized pulp without traces of ink in the supernatant;
- d) filtering the resulting pulp obtained from step (c) under suction to get the desired deinked paper.
2. A process according to claim 1, wherein the strain used has the following characteristics:
- a) it is a gram negative bacterium;
- b) it is a coccoid bacterium isolated from the coastal marine sediments of Dona Paula, Goa.
3. A process according to claim 1, wherein the said novel medium is prepared by dissolving the nutrient broth in sea water with salinity diluted to 50% and supplemented with tween 60 or 1% starch or both.
4. A process according to claim 1, wherein sodium chloride at a minimum concentration of 1.5% can be used in the place of sea water for growing the said bacterium.
5. A process according to claim 1, wherein the xerographic or inkjet-printed paper is soaked in hot water for 1-2 hours after addition of 1% surfactant followed by macerating the pulp.
6. A process according to claim 1, wherein the pulp sheet is pressed to make it uniform followed by drying at temperature ranging from 25° C. to 70° C. for a period of 4-5 hours.
7. A process according to claim 1, wherein the same batch of cell free culture supernatant can be effectively used for two-three cycles of deinking of xerox paper/inkjet-printed paper pulp.
8. A process according to claim 1, wherein the lyophilized culture supernatant can also be effectively used for deinking of the said pulp.
9. A process according to claim 1, wherein bacteria immobilized in sodium alginate beads can also be used for deinking the xerox paper/inkjet printed paper pulp to get completely bleached pulp within 48-72 hours.
Type: Application
Filed: Mar 30, 2006
Publication Date: Mar 27, 2008
Inventors: Raghu Kumar Chandralata (Dona Paula), Chellandi Mohandass (Dona Paula), Antonio Mascarenhas (Dona Paula), Vijay Kumar Kannoujia (Dona Paula)
Application Number: 11/394,137