MICROENCAPSULATION PROCESS OF SERTOLI CELLS, MICROCAPSULES OBTAINED AND USE FOR TREATMENT OF TYPE I DIABETES MELLITUS

Abstract

An apparatus for production of microcapsules includes a volumetric pump configured to deliver a polysaccaridic suspension through a catching tube. A needle-type element is configured to receive the suspension through the catching tube, and the needle-type element has a button hole opening in a lateral wall thereof and an output hole that outputs the suspension. The button hole opening receives a pressure fluid current. A pressure regulator is coupled to the button hole opening to regulate the pressure fluid current to interrupt a suspension flow and obtain microdroplets of homogeneous size exiting the output hole. The microdroplets are received in a receiving container in a solution including divalent cations or polycationic substances to form a gel such that homogeneous microcapsules are formed.

Description

The invention relates to the use of Sertoli cells (SC) microencapsulated into hydrogel-based microcapsules, for the prevention and/or treatment of Type 1 diabetes mellitus (T1DM) and to a process for producing microcapsules, preferably shaped as microspheres. The product object of the invention is capable of inducing both the neogenesis of beta-cells, destroyed by the diabetic pathology, and the “cutting off” of the same autoimmune process responsible for such destruction in T1DM.

The treatment with microencapsulated SC allows preventing and treating T1DM without resorting to any transplantation of hexogen pancreatic islets (either human or animal). It should be noted that the product obtained from SC microencapsulation is fully comparable to a “conventional” drug.

STATE OF THE ART

The worldwide current incidence of type 1 diabetes mellitus (T1DM) is equal to about 30,000 new cases a year. At the basis of type 1 DM pathogenesis which mainly but non exclusively affects young people and teenagers, is the destruction of most insulin-producing pancreatic beta-cells by an autoimmune mechanism. In short, the organism loses the immune tolerance towards the pancreatic beta-cells in charge of insulin production and induces an immune response, mainly cell-mediated, associated to the production of autoantibodies, which leads to the self-destruction of beta-cells.

The current T1DM therapy, based on the administration of hexogen insulin, tends to restore the glucide homeostasis as close as possible to that observed in physiological conditions. Insulin therapy, however, is not capable of reproducing the pulsating rhythm of insulin secretion typical of normal beta-pancreatic cell in response to secretagogue stimuli.

The restoration of a physiological and steady endocrine-pancreatic function would therefore represent the final goal for the radical solution of the pathology. To this end, new strategies have been proposed, such as the transplantation of whole pancreas or that of islets isolated from pancreas of human donors.

The hexogen insulin therapy currently used does not represent in any way the final therapy for treating T1DM. To overcome this problem, approaches have long been proposed which envisage the transplantation of the entire pancreatic organ or that of islets separated from the pancreas of human or animal donors.

The transplantation of islets, compared to that of the whole pancreas, is less invasive but exhibits similar problems, and in particular:

1. Reduced availability of human pancreas from dead donors, and, as a consequence, of islets.
2. Need of subjecting the recipient to lifelong general pharmacological immunosuppression regimes. Such therapeutic option used to prevent the immune rejection of the transplanted tissue, however, is burdened by side effects that are still little known nowadays, but also potentially very serious.
3. Rejection of transplants of heterologous islets, since none of the immunosuppressive drugs currently used has proved capable of effectively preventing them.
4. Poor survival of the transplanted islet tissue over time.

The Sertoli cell (SC) has recently been revaluated in its functions and promoted from a mere structural support of the testicular seminiferous tubule to a real biochemical laboratory with countless trophic and immunological functions. In particular, it has been proved that SC cultures produce molecules that inhibit the proliferation of B and T lymphocytes (1). Moreover, to strengthen their immunoregulatory function, the SC can induce the apoptosis of T, B cells and natural killers, linking through their ligand FAS to the FAS expressed by the target cells (2).

Another mechanism through which the SC carry out their immunomodulating role is represented by the production of Transforming Growth Factor-β (TGF-β) (3). This molecule affects the phenotype of differentiation of T CD4+ lymphocytes, favouring type Th2 (protective immunity) over type Th1 (non protective immunity). As a whole, the Sertoli activity may therefore have a direct clinical importance in T1DM, since beta cells are destroyed by an infiltrate mainly consisting of lymphocytes Th1 (INF-gamma positive).

The immunoregulating effect of SC, moreover, is associated to the production of several growth factors, differentiating and anti-apoptosis such as transforming growth factor (TGF-□), Glial Derived Neuroprophic Factor (GDNF), interleukin-1 (IL-1), stem cell factor (cKit-ligand), Fas/Fas Ligand (Fas-L), activin A and finally BCL-w (4).

The closest prior art (bibliographic reference No. 5) describes the introduction of SC into ultrapure alginate microcapsules with the obtainment of microcapsules with a mean end diameter of 520±14 μm.

At the time of such article, at an international level, microcapsules were considered satisfactory with a diameter of about 500 μm and a percentage of “tails” not higher than 5%. Both the capsular diameter and the presence of tails are very important parameters. The first one, to be reduced as much as possible, to allow a more effective exchange of metabolites; the second one as it has recently been found that even a percentage of tails <5% could trigger important phlogosis due to the creation of “loci minoris resistentiae” wherein cellular antigens may be exposed.

The inventors of the present invention have surprisingly found a process that allows producing homogeneous microcapsules of smaller dimensions without the presence of tail structures that can encapsulate SC while keeping their vitality and functionality unaffected.

In consideration of the above, the invention proposes for the first time the possibility of preventing and/or treating T1DM by transplanting SC microencapsulated into hydrogel-based microspheres, without any presence of hexogen islet tissue.

The object of the present invention therefore is a process for the manufacture of hydrogel-based microcapsules, containing Sertoli cells (SC) according to claim 1.

BRIEF DESCRIPTION OF THE DRAWINGS

Nine figures are attached to the present description, which show:

FIG. 1. Microphotographs of swine SC. A: Immunocytochemistry, obtained by incubating the preparation with anti-mullerian inhibiting factor (MIS) antibodies. B: Immunocytochemistry, obtained by incubating the preparation with anti-vimentin antibodies. C-D: To prove the poor presence of Leydig cells and peritubular cells, the preparation was subject to histochemical techniques to assess the presence of alkaline phosphatase, colouring with Fast-Red (typical of peritubular cells) (C), and of 3-β-hydroxy-steroidodehydrogenase activity, colouring with Nitro-blue tetrazolium (typical of Leydig) (D).

FIG. 2. Apparatus for the production of SC microencapsulated into alginate-based hydrogels through “air-monojet” (Panel A). Panel B shows the most important components of the devised system.

FIG. 3. Microphotographs of alginate-based microparticles obtained by the atomising system “air monojet”, using BaCl₂ (A-C) and CaCl₂+polyornithine (B-D) as gelling agents.

FIG. 4. Microphotographs in clear field of polysaccharidic microparticles cross-linked with Barium (A) and Calcium (B) ions after recovery from the peritoneal cavity of NOD rats 4 months after the implant. Panel C shows a microphotograph obtained by a fluorescence microscope of SC microencapsulated in barium alginate after a dual colouring with EB/FDA.

FIG. 5. Percentages of spontaneous onset of T1DM (85%) declared by the supplier of NOD rats (Taconic) (A), comparable to those shown by the pre-diabetic animals of the “naive” control group treated with empty microcapsules (B). On the other hand, panel C shows how the pre-diabetic animals treated with encapsulated SC have a percentage of onset of T1DM considerably reduced, equal to just 9% (preventive effect).

FIG. 6. Values of post-transplant mean glycaemia in NOD rats (Group E), with evident spontaneous diabetes treated with microencapsulated SC (therapeutic effect).

FIG. 7. Analysis RT-PCR on splenocytes of animals treated with microencapsulated SC. The results show that in animals from Groups C and E (see section VII), the treatment with SC can increase the number of positive in vivo Foxp3 cells. This result denotes an important increase of T cells with regulating features, that is, capable of regulating the activation and the proliferation of several cells involved in immune responses.

FIG. 8. Histological analysis of pancreatic islets of pre/diabetic NOD rats (A) and rats with spontaneous diabetes (B). The images show that the islet is totally free from both perk and intrainsular insulitic infiltrate. Panels C and D show the histological analyses of islets of pre-diabetic “naive” NOD rats (C) and suffering from spontaneous diabetes (D) treated with empty capsules.

FIG. 9. Layout of the device according to the invention.

DESCRIPTION OF THE INVENTION

In the first place, a homogeneous polysaccharide suspension of SC is produced: the solution has a 90% purity in terms of cellular composition and is obtained in a saline solution of ultrapure sodium alginate at a concentration comprised between 1 and 5% w/v, advantageously between 1 and 3%. The alginate used is ultrapure as it exhibits an endotoxin content not higher than 20 EU/g and a protein content <0.4%; air is advantageously used as fluid. SC are treated in advance with trypsin and EDTA (2 min), in order to obtain a homogeneous cellular suspension. The following were used to assess it:

• • immunocytochemistry techniques, incubating the preparation with anti-mullerian inhibiting factor (MIS) and fluorescin anti-vimentin antibodies, which respectively mark the MIS and vimentin molecules, both expressed by the SC only.
  • histochemical techniques to assess the presence of both alkaline phosphatase (colouring with Fast-Red) typical of peritubular cell, and of the 3-β-hydroxy-steroidodehydrogenase enzyme (colouring with Nitro-blue tetrazolium) which on the other hand is typical of Leydig cells.

The results obtained with such histochemical assays have allowed to prove the presence of 5-8% of peritubular and Leydig cells; these cellular populations, moreover, are useful (when present in these proportions) to ensure a molecular “cross-talk” favourable for the correct functionality of SC.

This suspension is aspired at a speed comprised between 10 and 60 ml/min producing a continuous flow of dimensionally homogeneous microdroplets through suction and extrusion using a fluid current, advantageously air, at controlled pressure. The suspension thus aspired is introduced in a needle-type element to be divided into highly homogeneous microdroplets. Advantageously, the needle-type element exhibits a buttonhole opening on the side surface thereof wherein a fluid current is introduced at a rate of 3-7 litres/min to obtain a continuous flow of homogeneous size microdroplets. The fluid current is obtained from a generator and before being used, it is subject to a pressure reduction to obtain a pressure drop in the flow-non flow transient not less than 0.3 Bar; to a regulation to obtain high reproducibility in the flow-non flow transient and linearity between number of revolutions and fluid current dispensed, and to a regulation and control of the output current between 0 and 10 NL/min.

The microdroplets may have a mean diameter comprised between 300 and 700 μm with a standard deviation below 40 μm. The microdroplets obtained are introduced in an aqueous solution, advantageously using sterilised water for injectable preparations, F.U, containing divalent cations or polycationic substances with resulting gelification and obtainment of said microcapsules.

A further object of the present invention are Sertoli cells as sole therapeutic agent for the prevention and radical cure of T1DM.

Advantageously, according to the process of the invention, the aspiration takes place continuously by a peristaltic pump at a flow speed comprised between 10 and 16 ml/min and said extrusion takes place through the “air monojet” system using a fluid flow, preferably air, comprised between 3 and 7 l/min. In the process, the exact calibration of said air flow, a characterising element of the entire method, is ensured by the below components of the system that are not present in previous methods (including that used in the “Closest Prior Art”). Before coming into contact with said suspension of said stage b) said air flow is subject to the following operations with the following devices:

• • the membrane pressure reducer Swagelok (KPR1JRF411A20000) which is capable of ensuring high reproducibility of the output pressure and so as to obtain a very low pressure drop in the flow/non flow transient below 0.3 Bar, serves for stabilising and making the air flow to be sent to the extruder reproducible;
  • regulation through a micrometric valve Swagelok (SS-SS6MM), in output to the pressure regulator, which allows regulating very finely the output air flow (0-10 NL/min) with a high reproducibility in the flow/non flow transient and maintaining linearity between number of revolutions and dispensed flow;
  • with rotating float flow meter (ROTAMETRO) Yokogawa, supplied by Precision Fluid (RAGK41-TOSS-SSNNN-M741A-TTCGN*A), located downstream of the micrometric valve, which allows a precise and quick reading of the output flow (0-10 NL/min) and thus the adjustment thereof through the micrometric valve.

A further object of the present invention are microcapsules containing SC obtainable according to the process of the invention, one of the features thereof is to exhibit the secretion of IGF-1 of microencapsulates SC identical to that of non-microencapsulates or “free” SC.

A further object of the present invention is the use of Sertoli cells, advantageously microencapsulated according to the process of the invention, as sole therapeutic agent for the production of a medicament of the prevention and radical cure of T1DM.

According to the invention, the microcapsules obtained can be subject to washing operations and/or further coating with natural and/or artificial polymers.

Compared to the prior art, the process of the invention allow a) producing microcapsules of smaller size, with fixed diameters (starting from 300 μm) and perfectly homogeneous, without the presence of “tails” structures and above all, without loss of vitality and functionality of the microencapsulated SC; b) increasing the number of microencapsulated SC by ml of alginate from 10⁶ SCs to 20⁶ SC by ml of alginate with imaginable implications on the possibility of implanting a larger number of SC in the smallest possible volume of polymer and c) increasing the functionality of microencapsulated SC, in particular relating to the production of IGF-1, the secretion thereof changes from 50 ng/ml/20×10⁶ cells) to 80 ng/ml/20×10⁶ cells substantially equal to that of “free” SC.

With reference to the present invention, it should be noted that

• • 1. For the first time, microencapsulated SC are proposed as final therapeutic approach, inducing the neogenesis of patient's beta-cell, destroyed by the autoimmune process.
  • 2. An optimisation of the microencapsulation process has been obtained which leads to the production of microcapsules with improved features, such as the reduction of mean dimensions, the reduced polydispersity and the absence of morphological deformities of the microcapsules (“tails” and coalescences).

A further object of the present invention are compositions comprising SC contained in microcapsules obtainable by the process of the invention together with physiologically tolerable carriers to use for the prevention and treatment of T1DM. An example of carrier consists of saline for intraperitoneal administration.

Below are the detailed aspects of the present invention.

Purification of Polymers

The polymers usable for microencapsulating the SC are not available on the market in the highly purified form strictly necessary for applications requiring parenteral administrations, such as human transplants. In these cases, in fact, strict internationally recognised criteria of “quality control” are required (see guidelines of the Ministry of Health and of U.S. Pharmacopeia).

Most commercial products, in fact, have quite high endotoxin levels (generally comprised between 30,000 and 60,000 EU/g) which make them totally unsuitable for transplant procedures, which require endotoxin levels not higher than 100 EU/g. As a consequence of the above, all the polymers used for producing microparticles are suitably subject to subsequent purification cycles that allow the drastic reduction of the endotoxins present.

Isolation of SC

The SC may be isolated and purified from various animal sources, generally prepuberals. After anaesthesia, the animals are subject to bilateral orchiectomy. After the removal of the epididymis, the testicles are subject to multienzymatic digestion. Once the digestion is complete, the tubular tissue is subject to filtration. The tubules thus obtained are placed in a culture at 37° C. in a 5% atmosphere of CO2. After 48 hours in incubator, the SC start adhering to the culture plates, forming a cellular monolayer. The SC obtained are analysed in terms of purity, vitality and functionality. The cellular vitality test is routinely conducted immediately after the isolation, on the second day of culture and immediately before and after the microencapsulation process.

Production of Microencapsulated Sertoli Cells

The SC may be immobilised into microcapsules consisting of various hydrogels consisting of hydrophilic polymers used alone or in mixtures. The initial phase of the microencapsulation process envisages the obtainment of a continuous and calibrated flow of microdroplets. Various procedures may be used for obtaining the microdroplets: (a) “air-monojet” microencapsulator, (b) automatic vibrating encapsulator, (c) electrostatic microencapsulator e (d) microfluidic lab-on-a-chip systems.

Once a flow of microdroplets with controlled and homogeneous dimensions is obtained, these are transformed into solid microspheres through gelification procedures. For example: converging monolayers of SC are treated to obtain a homogeneous cellular suspension, the SC are resuspended in the various ultrapure polymeric solutions (obtained as described in section “Purification of polymers”) and finally, the microcapsules obtained in the gelling bath are washed and isolated. The microcapsules produced may be used as such or be further coated with various natural, semi-synthetic or synthetic polymeric layers. The method proposed therefore allows (as shown by the pictures of FIG. 3) immobilising the SC into microcapsules with highly homogeneous dimensions, without morphological defects (presence of coalescences or “tail” structures), ensuring that the vitality and functionality features of the encapsulated cells are maintained.

In Vivo Biocompatibility of Encapsulated SC

The microparticle biocompatibility is assessed through the intraperitoneal implant carried out through abdominal incision. The body weight of each recipient animal is monitored during all the in vivo study. At different times from the transplant, the microcapsules are explanted to assess their morphology and function of the encapsulated cells. The general features of the recovered microspheres were determined through microscopic analysis, assessing both the morphology and any presence of inflammatory cells of the capsule surface. The vitality of microencapsulated SC was also assessed using the dual colouring technique with EB/FDA.

Assessment of the In Vivo Activity of Microencapsulated SC

It has been proved that the intraperitoneal transplant of microencapsulated SC in saline is capable of both preventing and treating T1DM in “stringent” animal models of human T1DM, such as NOD rats. Advantageously, but not exclusively, the administration of the product obtained from the microencapsulation of SC according to the invention takes place by intraperitoneal administration, with the product carried in saline.

A further object of the invention is a device for producing microcapsules advantageously for applying the process of the invention. The device and the operation thereof shall now be described with reference to FIG. 9. A first container 2 cooperates with flow dispensing means 4, advantageously a volumetric pump, for delivering suspension 1 through the catching tube 3 to a needle-type element 5. The needle-type element 5 exhibits a buttonhole opening 6 in the lateral wall thereof and output hole 7. A joint 8 allows a pressure fluid current 10, preferably air, coming from a generator 9 and regulated by adjusting means 11, to enter inside element 5. By suitably regulating current 10 it is possible to interrupt the suspension flow and obtain microdroplets 13 of homogeneous size, which form gel in a solution containing divalent cations present in a second container 12. The airjet instrument mentioned above and the conditions described are applied for obtaining the homogeneous microcapsules.

Development of a Prototype of Microencapsulator Usable in Sterility Conditions and GLP

EXAMPLES

Microencapsulation of Sertoli Cells Into Alginate-Based Microspheres and Assessment of the In Vivo Biocompatibility and Functionality

Purification of the Polymer

Sodium alginate obtained through a process of sequential filtrations, was used as base polymer for the production of microcapsules, usually available in a 1-6% (w/v) solution, appropriately stored in a place protected from light and at a temperature of 4°-6° C. Said compound is stable over time for about 5 years, has an endotoxin content not higher than 20 EU/g and a virtually absent protein content (<0.4%—another criterion of “bioinvisibility” of U.S. FDA).

Isolation of SC from Prepuberal Baby Swine

The SC were isolated from testicles of baby swine (7-15 days old) “Large-White”. After anaesthesia through the i.m. administration of 0.1 mg/kg azaperon (Stresnil® 40 mg/ml, Janssen, Brusselle, Belgium) and 15 mg/kg ketamine (Imalgene® 100 mg/ml, Gellini Farmaceutici, the swine were subject to bilateral orchiectomy. After the removal of the epididymis, the testicles are decorticated from the albuginea, finely chopped into small tissue fragments (1-3 mm3) and immediately subject to a first enzymatic digestion based on collagenase P (Roche Diagnostics, S.p.A., Monza, Italy) (2 mg/ml) in HBSS (Sigma Chemical Co, St. Louis, USA). The digestion is continued up to the separation of the seminiferous tubules. The collected tubules are then washed in HBSS and centrifuged at 500 r.p.m. After the wash, the tubules are incubated with a solution of HBSS containing trypsin (2 mg/ml) and DNAse I (Sigma). After the completion of the second digestion, the trypsin solution is diluted 1:1 with Hank's+20% FBS to stop the enzymatic activity thereof. After further washes with HBSS, the tubules are separated from the peritubular cells through a light centrifugation at 300 rpm. The “pellet” containing the tubular tissue is suitably filtered with a stainless steel filter with a 500 μm mesh opening. Finally, in order to remove any peritubular and Leydig cells contaminating the preparation, the suspension containing the tubules is further centrifuged at 800 rpm for 5 min and the resulting pellet is treated for 7 min with a glycine 1 M solution and EDTA 2 mM in HBSS at pH 7.2.

The tubules thus obtained are placed in a culture in HAM F12 (Euroclone) supplemented with retinoic acid 0.166 nM (Sigma) and with 5 ml/500 ml insulin-transferrin-selenium (ITS) (Becton Dickinson#354352), at 37° C. in a 5% atmosphere of CO2. After 48 hours of culture, the SC start adhering to the culture plates, forming a cellular monolayer. In order to remove the residual germ cells (which, as known, if implanted in the peritoneal cavity may give rise to dysgerminoms), the SC monolayers are treated with a buffer, tris-(hydroxymethyl)-aminomethane hydrochloride (TRIS) (Sigma) that allows eliminating the residual germ cells through osmotic lysis. Finally, the SC are grown in the above conditions, usually in 75 cm2 flasks.

The SC obtained were analysed in terms of purity, vitality and functionality. The purity of the SC, which was higher than 90%, was assessed by immunocytochemistry techniques, incubating the preparation with anti-mullerian inhibiting factor (MIS) and fluorescin anti-vimentin antibodies, which respectively mark the MIS and vimentin molecules, both expressed by the SC only (FIG. 1 A, B).

To prove the reduced presence of Leydig and peritubular cells as possible contaminants, the SC preparations were subject to histochemical assessments. These tests allow assessing both the presence of alkaline phosphatase (colouring with Fast-Red) typical of peritubular cell, and of the 3-β-hydroxy-steroidodehydrogenase enzyme (colouring with Nitro-blue tetrazolium) which on the other hand is typical of Leydig cells (FIG. 1 C, D). The results obtained with these histochemical assays have allowed to prove the presence of 5-8% of peritubular and Leydig cells; these cellular populations, moreover, are useful (when present in these proportions) to ensure a molecular “cross-talk” favourable for the correct functionality of these populations of testicular cells.

The vitality of SC was determined by treatment with ethidium bromide (EB) and fluorescein-diacetate (FDA) (Sigma). The cells, observed by a fluorescence microscope, in all conditions showed a vitality higher than 95%. The cellular vitality test is routinely conducted immediately after the isolation, on the second day of culture and immediately before the microencapsulation process.

C) Production of Microdroplets for Encapsulating Sertoli Cells

The SC were immobilised into microcapsules consisting of various polysaccharide polymers used alone or in mixtures. The selected polymer was sodium alginate ultrapurified at our laboratories. The initial phase of the microencapsulation process envisages the obtainment of a continuous and calibrated flow of microdroplets starting from cellular suspension of SC in an aqueous polysaccharide suspension with a polymeric concentration variable between 1 and 5% (w/v).

Various procedures were and may be used for obtaining the microdroplets: (a) “air-monojet” microencapsulator, (b) automatic vibrating encapsulator, (c) electrostatic microencapsulator e (d) microfluidic lab-on-a-chip systems.

In particular, the method selected (a), based on a semi-automatic, compact, sterilisable and transportable microencapsulator (FIG. 2, A shows an overall view of the system), has allowed producing microcapsules containing SC with highly homogeneous dimensions (300 to 700 μm diameter), without any evident morphological flaw (such as the presence of coalescences or “tail” structures) and above all, without the loss of vitality and functionality of the microencapsulated SC.

Panel B of FIG. 2 schematises the procedure of the microencapsulation process through “air-monojet”.

D) Preparation of Ultrapurified Alginate-Based Microcapsules

Once a flow of microdroplets with controlled and homogeneous dimensions is obtained, these are transformed into solid microspheres through a gelification procedure which envisages the forming of ionic links with divalent ions according to a method developed and validated at our laboratories.

In particular, converging monolayers of SC are treated with 0.05% trypsin/EDTA (Gibco, Grandisland, USA) (2 min), in order to obtain a homogeneous cellular suspension. Once washed, the SC are counted by hemocytometric analysis and tested for vitality. Afterwards, the SC are resuspended in the various ultrapure polymeric suspensions in concentrations variable between 1.5-2% (w/v) of AG. For the production of microcapsules with the “air-monojet” system, the SC suspension in the polymers is continuously aspired by a peristaltic pump at a flow speed comprised between 10 and 16 ml/min. The cellular suspension is then extruded through the “air monojet” system (using an air flow comprised between 3 and 7 l/min). During the entire process, the SC suspension is kept under light stirring to prevent the cellular aggregation and the possible formation of microcapsules with non-homogeneous distribution of SC therein.

The microdroplets produced are gellied with a solution containing divalent cations, such as Ca+2 or Ba+2 (0.5-2.5%, w/v). In this way, the microdroplets are instantly transformed into gel microspheres. Afterwards, the microcapsules are left to settle for periods variable between 2 and 15 min into the gelling bath. At the end of this step, the microcapsules are subject to repeated washing cycles with saline.

The microcapsules produced may be used as such or be further coated through sequential incubation in solutions containing natural, semi-synthetic or synthetic cationic polymers. For example, poly-L-ornithine (PLO) was used at 0.12% (for 10 min) and at 0.06% (for 6 min). Finally, the microcapsules coated with PLO are further treated with a diluted solution of polysaccharide, to provide the highly biocompatible final outer coating.

FIG. 3 shows the microphotographs of alginate-based microcapsules obtained by the procedure described above, both using only Barium ions (A-C) and the procedure of the multiple coating with Calcium/polyornithine/polymer ions (B-D). The method proposed therefore allows (as shown by the pictures of FIG. 3) immobilising the SC into microcapsules with highly homogeneous dimensions, without morphological defects, such as the presence of coalescences or “tail” structures, and finally, ensuring that the vitality and functionality features of the encapsulated cells are maintained.

E) In Vivo Biocompatibility of Encapsulated SC

After general anaesthesia, induced by intra-peritoneal administration of 100 mg/kg ketamine (Parke-Davis/Pfizer, Karlsruhe, Germany) and 15 mg/kg xylazine (Bayer, Leverkusen, Germany), the alginate microparticles were introduced through a small abdominal incision in the peritoneal cavity of female NOD rats (Harlan, Italy, approximate weight of 25 g). 106 microencapsulated SC were implanted in each animal. The body weight of each recipient rat was monitored during all the in vivo study.

After 4 months from the transplant, the microcapsules were explanted, after anaesthesia, from the peritoneal cavity of the animals to assess their morphology and function of the contents. The microcapsules were recovered by peritoneal wash using saline. The general features of the recovered microspheres were determined through microscopic analysis, assessing both the morphology and any presence of inflammatory cells of the capsule surface. The vitality of microencapsulated SC was also assessed using the dual colouring technique with EB/FDA.

The microphotographs shown in FIG. 4 (A-B) show that the polysaccharidic microparticles keep high biocompatibility standards, as shown by the minimum levels of inflammatory cells present on the capsular surface. Moreover, the microencapsulated SC both in Barium (FIG. 4A) and calcium (FIG. 4B) alginate, keep excellent vitality levels 4 months after the implantation (FIG. 4 C).

E) Assessment of the In Vivo and In Vitro Activity of Microencapsulated SC

The present invention finds application in the field of transplantation biotechnologies, such as for example the prevention and treatment of T1DM. Actually, at our laboratories we have proved that the intraperitoneal transplant of microencapsulated SC in barium alginate microspheres (206/rat) is capable of both preventing and treating T1DM in “stringent” animal models of human T1DM, such as NOD rats. In particular, SC microencapsulated into barium alginate (BaAG) microspheres were transplanted, after 72 hours culture, in the peritoneal cavity of pre-diabetic NOD rats and affected by evident diabetes. The implantation was carried out in a general anaesthesia through laparotomy. The transplanted animals were then monitored with weekly checks for their body weight and glycaemia before and after meals. The experimental protocol we followed envisaged groups of animals subject to different treatments as indicated below.

Group A: “naive” pre-diabetic control animals (treated with empty microcapsules).

Group B: control animals (treated with empty microcapsules) with spontaneous diabetes.

Group C: “naive” pre-diabetic animals treated with intraperitoneal implant of microencapsulated SC.

Group D: “naive” pre-diabetic animals treated with intraperitoneal implant of “free” SC: (206/rat).

Group E: animals with spontaneous diabetes treated with intraperitoneal implant of microencapsulated SC.

During the course of the in vivo study, some animals were sacrificed to assess the peripheral immunological layout through collection of spleen, peripancreatic lymph nodes and pancreas with concurrent histomorphological and immunocytochemical examinations.

The complete analysis of in vivo experiments on NOD rats has allowed proving that microencapsulated SC transplanted in pre-diabetic animals suffering from spontaneous diabetes allowed obtaining, important therapeutic results, as shown below.

(A) Microencapsulated SC are capable of preventing the onset of T1DM in NOD rats. This sensational result can be obtained from the analysis of the percentages of spontaneous onset of T1DM. In fact, this pathology occurred spontaneously in 85% of the animals in group A (FIG. 5 B). This result is perfectly in line with the percentages of occurrence of T1DM declared by the supplier of NOD rats (Taconic) (FIG. 5 A).

On the other hand, in the animals of group C (the pre-diabetic ones treated with encapsulated SC), the percentage of onset of T1DM was only 9% (FIG. 5 C). Finally, in the animals of group D (pre-diabetic treated with intraperitoneal implant of “free” SC), the percentage of onset of T1DM was greatly reduced compared to that of the “naive” (19%), although higher than in the animals of group C (FIG. 5 D).

(B) The microencapsulated SC are capable of normalising, in just 7-15 days from the implant, the glycaemic values (with the attainment of glycaemia below 200 mg/dl) in more than 60% of rats in Group E (N=30) that had spontaneously developed diabetes (FIG. 6 A). On the other hand, the animals in Group B (diabetics treated with empty capsules) always kept high levels of glycaemia, dying quickly, in 1-2 weeks. Finally, the animals in group F (N=30) (diabetics treated with intraperitoneal implant of “free” SC) were able to normalise the glycaemic values although in a lower percentage, equal to about 40%.

(C) Studies carried out on lymph nodes, pancreas and spleens have shown that the SC are capable of “re-educating” the immune system of the animals in Groups C and E, “blocking” the autoimmune attack responsible for the disease, as can be seen from FIG. 7 relating to real time Polymerase chain reaction (PCR) results on the splenocytes of treated animals.

In particular, such results show that one of the main effects of the treatment with SC is their capacity to induce in vivo Foxp3+ cells. Foxp3 is a selective marker of T cells with regulating features, that is, capable of regulating the activation and the proliferation of several cells involved in immune responses and the number whereof is reduced in the NOD rat model.

(D) Histochemical assays carried out on all the groups of animals studied show that the treatment with SC is capable of removing the insulitic mononuclear infiltrate at the pancreas level in animals of groups C and E compared to those of the control groups (A and B) (FIG. 8). Moreover, such effect was followed by the activation of pancreatic mesenchymal stem cells capable of generating new β-cells which are capable of normalising glycaemia in animals treated with SC, as they are not undermined by the autoimmune attack anymore. The remodulation of the immune response after treatment with SC is mediated by the activation of the immunoregulatory pathway of the indoleamine 2 3-dioxygenase (IDO) enzyme, an isoform whereof is expressed and functioning in SC, too.

BIBLIOGRAPHIC REFERENCES

• • 1. DeCesaris P, Filippini A, Cervelli C, Riccioloi, A.; Muci, S.; Starace, G.; Stefanini, M.; Ziparo, E. Immuno-suppressive molecules produced by Sertoli cells cultured in vitro: Biological effects on lymphocytes. Biochem. Biophys Res. Commun. 1992, 186: 1639-1646.
  • 2. Lynch D H, Ramsdell F, Alderson M R. Fas and FasL in the homeostatic regulation of immune responses. Immunol. Today 1995, 16: 569-574.
  • 3. Suarez-Pinzon W, Korbutt G S, Power R, Hooten J, Rajotte R V, Rabinovitch A. Testicular Sertoli cells protect islet B-cells from autoimmune destruction by a transforming growth factor-βl-dependent mechanism. Diabetes 2000, 49:1810-1818.
  • 4. Emerich, D. F., Hemendinger, R., and Halberstadt, C. R. The Testicular-Derived Sertoli Cell: Cellular Immunoscience to Enable Transplantation. Cell Transplantation 12, 335, 2003.
  • 5. Luca, G., Calvitti, M., Nastruzzi, C., Bilancetti, L., Becchetti, E., Mancuso, F., Calafiore R. Encapsulation, in Vitro Characterization and in Vivo Biocompatibility of Sertoli's Cells in Alginate Based Microcapsules. Tissue Eng. 2007, 13:641-648.

Claims

1.-13. (canceled)

14. An apparatus for production of microcapsules, comprising:

a volumetric pump configured to deliver a polysaccaridic suspension through a catching tube;

a needle-type element configured to receive the suspension through the catching tube, the needle-type element having a button hole opening in a lateral wall thereof and an output hole that outputs the suspension, the button hole opening receiving a pressure fluid current;

a pressure regulator coupled to the button hole opening to regulate the pressure fluid current to interrupt a suspension flow and obtain microdroplets of homogeneous size exiting the output hole, the microdroplets being received in a receiving container in a solution including divalent cations or polycationic substances to form a gel such that homogeneous microcapsules are formed.

15. The apparatus as recited in claim 14, wherein the polysaccaridic suspension includes a homogeneous suspension of Sertoli Cells (SC) with purity, in terms of cell composition, higher than 90%, in a saline solution of ultrapure sodium alginate.

16. The apparatus as recited in claim 15, wherein the needle-type element extrudes the suspension through the output hole to obtain a continuous flow of microdroplets showing homogeneous dimensions.

17. The apparatus as recited in claim 16, wherein the homogeneous dimensions include the microcapsules with substantially fixed diameters and without tails structures.

18. The apparatus as recited in claim 16, wherein the homogeneous dimensions include the microdroplets having a mean diameter of between about 300 and 700 microns with a standard deviation below 40 microns.

19. The apparatus as recited in claim 14, wherein the microcapsules include sodium alginate, at a concentration of 1-3% w/v, with an endotoxin content not exceeding 20 EU/g and a protein content lower than 0.4%.

20. The apparatus as recited in claim 14, further comprising a fluid generator coupled to a supply side of the pressure regulator and having a pressure reduction to obtain a pressure fall of at least 0.3 bars in a flow/no flow transient of fluid current.

21. The apparatus as recited in claim 19, wherein the fluid generator includes a linear relationship between pressure cycles and amount of fluid dispensed.

22. The apparatus as recited in claim 14, wherein the microcapsules each include at least 20×106 Sertoli Cells (SC).

23. The apparatus as recited in claim 14, wherein the microcapsules including the Sertoli Cells are suitable as a sole therapeutic agent for the prevention and treatment of Type 1 diabetes mellitus (T1DM).

24. An apparatus for production of microcapsules including Sertoli Cells, comprising:

a volumetric pump configured to deliver a polysaccaridic suspension through a catching tube, wherein the polysaccaridic suspension includes a homogeneous suspension of Sertoli Cells with purity, in terms of cell composition, higher than 90%, in a saline solution of ultrapure sodium alginate;

a needle-type element configured to receive the suspension through the catching tube, the needle-type element having a button hole opening in a lateral wall thereof and an output hole that outputs the suspension, the button hole opening receiving a pressure fluid current wherein the needle-type element extrudes the suspension through the output hole to obtain a continuous flow of microdroplets showing homogeneous dimensions;

a pressure regulator coupled to the button hole opening to regulate the pressure fluid current to interrupt a suspension flow and obtain microdroplets of homogeneous size exiting the output hole, the microdroplets being received in a receiving container in a solution including divalent cations or polycationic substances to form a gel such that homogeneous microcapsules are formed; and

a fluid generator coupled to a supply side of the pressure regulator and configured to supply the pressure fluid current.

25. The apparatus as recited in claim 23, wherein the homogeneous dimensions include the microcapsules with substantially fixed diameters and without tails structures.

26. The apparatus as recited in claim 24, wherein the homogeneous dimensions include the microdroplets having a mean diameter of between about 300 and 700 microns with a standard deviation below 40 microns.

27. The apparatus as recited in claim 24, wherein the microcapsules include sodium alginate, at a concentration of 1-3% w/v, with an endotoxin content not exceeding 20 EU/g and a protein content lower than 0.4%.

28. The apparatus as recited in claim 24, wherein the fluid generator has a pressure reduction applied to obtain a pressure fall of at least 0.3 bars in a flow/no flow transient.

29. The apparatus as recited in claim 28, wherein the fluid generator includes a linear relationship between pressure cycles and amount of fluid dispensed.

30. The apparatus as recited in claim 24, wherein the microcapsules each include at least 20×106 Sertoli Cells (SC).

31. The apparatus as recited in claim 24, wherein the microcapsules including the Sertoli Cells are suitable as a sole therapeutic agent for the prevention and treatment of Type 1 diabetes mellitus (T1DM).

32. An apparatus for production of microcapsules including Sertoli Cells, comprising:

a container including a homogeneous suspension of Sertoli Cells with a higher than 90% purity, in terms of cell composition, in a saline solution of ultrapure sodium alginate;

a volumetric pump configured to aspirate the suspension to create a suspension flow;

a needle-type element configured to receive the suspension flow;

a pressure regulator coupled to the needle-type element and configured to regulate a pressure fluid current therethrough to interrupt the suspension flow and obtain microdroplets of homogeneous size exiting an output hole of the needle-type element, the microdroplets being received in a receiving container in a solution including divalent cations or polycationic substances to form a gel such that homogeneous microcapsules are formed; and

a fluid generator coupled to a supply side of the pressure regulator and configured to supply the pressure fluid current.

33. The apparatus as recited in claim 32, wherein the microcapsules have substantially fixed diameters without tails structures and have a mean diameter of between about 300 and 700 microns with a standard deviation below 40 microns.