FIELD OF THE INVENTION The present invention relates to novel nucleic acid sequences of specific Astigmata mite species, corresponding to nuclear ribosomal DNA (rDNA) that codes for ribosomal RNA. The invention further relates to the use of such sequences or fragments thereof in methods for the identification of the specific mite species in biological samples such as mass reared cultures, purified fractions from the cultures, house dust and other environmental samples.
BACKGROUND OF THE INVENTION Mites of the suborder Astigmata are recognised as important respiratory allergy causing elements. The most relevant species belong to the families Pyroglyphidae (Dermatophagoides and Euroglyphus), Acaridae (Acarus and Tyrophagus) and Glycyphagidae (Blomia, Glycyphagus and Lepidoglyphus). Allergen avoidance, drug therapy and immunotherapy are the main strategies currently conducted to reduce the allergic disease caused by mites, the latter being the only disease-modifying approach and the most promising to counteract allergy. The current immunotherapy involves administration to the patient of allergenic extracts in a suitable delivery form. In the case of mites, the extracts are produced from mass reared cultures of the relevant mites. Species identification and avoidance of cross contamination in mite cultures are key factors in the standardisation of allergen production. Furthermore, there is a regulatory requirement to certify the identity/purity/lack of cross-contamination of the mite cultures for preparing medical grade allergen extracts.
Also identification of mite populations in environmental samples from patients' houses is useful in order to monitor the risk of allergen exposure and for diagnostic purposes.
Mite species identification in mass reared cultures and in environmental samples has traditionally been based on morphological identification such as described in Spieksma 1990. The method is reliable but it can only be performed on samples of adult stages of intact mites and demands a high level of expertise. Morphological identification is time-consuming, represents an increased cost for the industry and cannot be applied on purified mite fractions downstream in the production process. Morphological identification of mite species in environmental samples can be challenging since the number of intact mites present may be quite low or even non existing.
Various molecular methods have been suggested to analyse phylogenetic relationships within phylogenetic orders of microorganisms, fungi, mites and ticks. In order to conduct phylogenetic studies, one or more suitable molecular markers must be identified. Cruickshank 2002 describes in a review article suitable properties of molecular markers and suggests nine possible molecular markers mainly selected within mitochondrial genes (mtDNA) and nuclear ribosomal genes (rDNA). Accordingly, the highly conserved regions of ribosomal DNA (18S rDNA, 5.8S rDNA and 28S rDNA), mitochondrial genes (cytochrome oxidase, 12S and 16S rDNA) and internal transcribed spacer regions of the rDNA (ITS1 and ITS2) have been proposed for phylogenetic studies.
Navajas 1999 assessed the usefulness of the molecular markers ITS1, ITS2 and 5.8S gene of rDNA for phylogenetic analysis and identification of species within Phytoseiidae mites. The entire ITS1-5.8S-ITS2 region was amplified with PCR (Polymerase Chain Reaction) using universal primers generated from the 18S and the 28S regions of rDNA. Each PCR-product was sequenced and aligned in order to determine the phylogenetic relationship. Navajas concluded that the level of DNA variation within a new group cannot be predicted and therefore preliminary assessment is necessary in order to identify suitable molecular markers for a species or a group of species. For Phytosiidae mites, ITS1 was longer than ITS2 and had much more sequence variation. ITS2 was considered too short to be of value in taxonomic studies and ITS1 was considered too variable, and 5.8S in combination with ITS2 was not considered giving adequate specificity within the group.
Noge 2005 used the ITS2 region of rDNA as molecular marker in order to make a phylogenetic analysis of 73 mite species. The primers for the PCR amplification were generated from the highly conserved regions flanking the ITS2 region (one in the 5.8S region and one in the 28S region). Three clones of each PCR-product were sequenced and aligned in order to determine the phylogenetic relationship.
Suarez-Martinez 2005 used mitochondrial 12S rRNA as a molecular marker in order to identify the four representative Astigmata mites Dermatophagoides pteronyssinus, Glycyphagus privatus, Aleuroglyphus ovatus and Blomia tropicalis. All species were amplified using one universal forward primer and one universal reverse primer generated from the rRNA 12S marker. Each PCR-product was sequenced and aligned in order to determine the phylogenetic relationship and to identify variants.
Some techniques for molecular identification of mite species in environmental samples have been proposed, such as PCR methods ((Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), multiplexPCR)) and arrays.
Wong 2011 successfully identified Dermatophagoides pteronyssinus, Dermatophagoides farinae, Blomia tropicalis, Tyrophagus putrescentiae, Aleuroglyphus ovatus and Glycycometus malaysiensis in house dust using the ITS2 region of rDNA as molecular marker in a RFLP PCR. The primers were generated from the highly conserved regions flanking the ITS2 region (one in the 5.8S region and one in the 28S region). After amplification, identification was performed by digesting the PCR products with a combination of restriction enzymes specific for the mite to be identified and separating the restriction fragments with SDS-PAGE. The restriction fragment size pattern was used to identify the mite species in question. Wong suggests isolating single mites if there are several different mites present in the same dust sample.
JP2007-202462, JP2008-35773 and JP2009-171986 all disclose various aspects of the same invention. The invention regards an array system based on nucleic acid hybridisation for detection or differentiation of mites and fungi in house dust samples as well as nucleic acid probes for use in the microarray. In brief, the entire ITS1-5.8S-ITS2 regions of mites and fungi were amplified from dust samples using mite-specific primers (SEQ ID NOs: 56 and 57) and fungi-specific primers (SEQ ID NOs: 58 and 59) all generated from the 18S and the 28S regions of the rDNA. The amplification of mites and fungi could be performed in a “1-tube PCR” in which mite-specific primers and fungi-specific primers were both added to the same tube. For each mite and fungus to be detected, nucleic acid probes were amplified from pure samples of the mite or fungus in question using primers generated either from each end of the ITS1 or from each end of the ITS2. The resulting probes of the invention thus correspond to either the ITS1 or the ITS2 of the species in question or to fragments thereof or to the complements thereof. In the microarray, each well identifies an ITS1 region of one species or an ITS2 region of the species or the complements thereof such that detection of one species uses four wells.
Thet-em 2012 designed a multiplex PCR using ITS2 and Cox I as molecular markers to identify Dermatophagoides pteronyssinus, Dermatophagoides farinae and Blomia tropicalis in house dust. Species specific primers for Dermatophagoides pteronyssinus and Dermatophagoides farinae were generated from the ITS2 region of rDNA. Species specific primers for Blomia tropicalis were generated from the Cox I gene of mitochondrial DNA.
None of these methods use primers for mite species identification designed on the ITS1 region. Further the methods all require a set of primers per DNA sequence to amplify. In the cases where the amplicons are large, it is necessary to subject the amplicons to various restriction enzymes and analyze the resulting patterns of the size distribution of the fragments obtained in order to identify the exact species by the molecular sizes of the amplicons. Most of the methods are only suitable to identify a single species in a sample.
Accordingly, fairly large quantities of samples are still necessary in order to identify several species in a sample using these methods. Finally, most of the methods include a first step of non-specific amplification using mite specific primers and a second step of quite complex processing of the amplicon to allow species identification (restriction enzymes in RFLP-PCR, sequencing or binding to probes in arrays).
Kumar et al 1999 developed a PCR multiplex technique for identifying Cecidophyopsis mites using species specific differences in rDNA ITS-1 sequences. Four PCR primers derived from ITS-1 were used for the simultaneous amplification (multiplex PCR) of interspecifically variable simple sequence repeats (vSSRs). The primers consist of two forward primers designed in 18S (M1) and in a first conserved area of ITS1 (M3) respectively and the two reverse primers are designed in the 5.8S (M4) and a second conserved area of ITS1 (M2). None of the primers are species specific. They are all mite specific (or common to all the mites) and amplify amplicons S1, S2 and S3 in all mite species. The differentiation between mite species is done by comparing the pattern of S1+S2+S3 to known patterns of mite species. Since some of the amplicons differentiate by only 1 bp, it is necessary to use polyacrylamide gels. Mites were identified by electrophoresing PCR products on polyacrylamide gels alongside those obtained from plasmids containing ITS copies of known mite species. The article mentions that “the patterns were not discernable in agarose gels”.
There is still a need for more simple and robust methods for the identification of one or more mite species in mite cultures for preparing allergenic extracts for diagnostic, prophylactic and therapeutic purposes as well as in house dust.
OBJECT OF THE INVENTION The inventors of the present invention have designed a method based on molecular markers in order to facilitate identification or certification of mite species in mass reared mite cultures or purified mite fractions thereof or in environmental samples. DNA markers have the advantage of neither requiring a given developmental stage, nor intact individuals for the morphological analysis or requiring special training of the staff. Further, the method is advantageous for performing routine mite species identification or certification of a large number of samples, since the method has low requirements to sample quality and quantity and it reduces the time and skills necessary to perform the identification of mite species in comparison to the morphological identification. A DNA marker appropriate for the species certification in the production of allergenic extracts should identify the mite species in either whole mite cultures or purified fractions of mites of mite bodies or mite faeces.
The inventors found the full-length ITS1, 5.8S sub-unit and ITS2 sequences of the rDNA from thirteen Astigmata species belonging to genera Dermatophagoides, Euroglyphus, Acarus, Tyrophagus, Glycyphagus, Lepidoglyphus and Blomia (families Pyroglyphidae, Acaridae, Glycyphagidae and Echymopididae). Based on the sequences obtained, a singleplex-PCR and multiplex-PCR method were developed to identify 10 of those species which are recognised as important respiratory allergy causing agents. Despite polymorphism and high variability in the ITS1 region, the inventors showed that a singleplex or a multiplex PCR method using primers designed on the ITS1 region of Astigmata mite species provides a simple, robust and reliable method of Astigmata mite species identification. In the multiplex PCR method, the primers may be combined for the simultaneous identification of multiple Astigmata mite species. The system can be used for species certification in mite cultures and purified fractions thereof (bodies and faeces) used for the industrial production of allergenic extracts. Finally, the system has been optimised for the detection of Astigmata mite species in environmental samples by introducing an optional preamplification step.
It is an object of the invention to provide sequence information for the full-length ITS1, 5.8S sub-unit and ITS2 sequences for specific Astigmata species providing new molecular markers for mite species identification or certification, as well as methods for the identification, detection, discrimination or differentiation of one or more different Astigmata mite species. It is also an object of the invention to use the sequence information both to design primers which are unique to a specific species (species specific) and to design primers which are specific to all Astigmata mite species (mite specific). It is a further object of the invention to provide singleplex and multiplex methods which are simple to perform and robust yet highly accurate.
SUMMARY OF THE INVENTION It has been found by the present inventors that the full-length ITS1, 5.8S sub-unit and ITS2 sequences of the rDNA from the specific Astigmata mite species may be used for the identification, detection or discrimination of these specific mite species.
So in a first aspect, the present invention relates to a method for the identification of one or more different Astigmata mite species in a sample, the method comprising the steps of:
a) obtaining DNA from the sample;
b) amplifying, such as by PCR, a region of the rDNA of each of the mite species to be identified using
-
- i. one or more, such as 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 first primers each first primer specifically hybridising to the ITS1 sequence of the rDNA of each of the mite species to be identified, or the complementary sequence thereof, and
- ii. one or more, such as one, second primers specifically hybridising to a sequence selected from any of the 18S, 5.8S or 28S sequences of the rDNA of the mite species to be identified, or the complementary sequence thereof,
to produce an amplicon specific to the mite species to be identified, and;
c) identifying the mite species by evaluating a characteristic of the amplicon.
It is to be understood in b) i. that “each first primer specifically hybridising to each of the ITS1 sequence of the rDNA of the mite species to be identified” means that each first primer hybridizes to only one sequence of a specific Astigmata mite species to be identified. Accordingly, if different species are present in a sample, each first primer will only hybridize to one specific species and not to the others. In a specific embodiment, the first primer is designed so that in addition to hybridizing to only one of the different Astigmata mite species to be identified, it will not hybridize to the ITS1 of any other known Astigmata mite species. Accordingly the first primer will only hybridize to the ITS1 of one specific known Astigmata mite species and will not be able to hybridize to identify any other known Astigmata mite species present in the sample or not.
It is to be understood in c) that when several mite species are present, several different amplicons are produced, each being specific for one particular mite species to be identified.
The sample may be any Astigmata mite containing sample such as a sample of a mass reared mite culture, a purified fraction thereof or an environmental sample. In particular, this method enables the identification of mite species in purified fractions of mass reared mite cultures.
Obtaining DNA from a sample is to be understood as extracting DNA according to methods known in the art, such as described in the examples, and in a form suitable for the subsequent amplification step.
The first primers may be forward primers and the second primers may be reverse primers or the opposite.
This method is highly sensitive, simple to perform, robust and provides a high degree of accuracy in identification of mite species in samples.
The polymerase chain reaction (PCR) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating from thousands to millions of copies of a particular DNA sequence. The technology is well known to the person skilled in the art.
In brief, the method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplification. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the DNA template is exponentially amplified. PCR can be extensively modified to perform a wide array of genetic manipulations.
Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the bacterium Thermus aquaticus. This DNA polymerase enzymatically assembles a new DNA strand from DNA building-blocks, the nucleotides, by using single-stranded DNA as a template and DNA oligonucleotides (also called DNA primers), which are required for initiation of DNA synthesis. The vast majority of PCR methods use thermal cycling, i.e., alternately heating and cooling the PCR sample through a defined series of temperature steps. In the first step, the two strands of the DNA double helix are physically separated at a high temperature in a process called DNA melting. In the second step, the temperature is lowered and the two DNA strands become templates for DNA polymerase to selectively amplify the target DNA. The selectivity of PCR is achieved by the use of primers that are complementary to the DNA region targeted for amplification under specific thermal cycling conditions.
The designing of primers and the optimization of the PCR conditions are key factors for the specificity and efficiency of the PCR as the skilled person will know. The temperature of the PCR should be optimised in accordance with the melting temperature of the primers (Tm, a measure of the stability of the duplex formed by hybridisation of the primer with their complementary sequence).
Various software tools are available to propose theoretical primers for a target DNA, or guidelines in textbooks may be followed. The composition of a primer affects the melting temperature and the ability of the primer to hybridise to a target DNA, and especially the 3′ end of the primer should have exact complementarity to the target DNA.
In an embodiment, the one (or more) first primers is a species specific primer. In a further embodiment, the one (or more) second primers is one common primer specific to Astigmata mites. In a preferred embodiment, the one (or more) first primers is species specific and the one (or more) second primers is one common primer specific to Astigmata mites. Such embodiment has the advantage that the number of different primers used may be reduced if several Astigmata mite species are to be identified in a single assay, such as in a multiplex-PCR. Furthermore, when the second primer is one common primer for several mite species to be identified, such that the amplicons produced has one common starting or ending point, it becomes more straight forward to design primers for the other end which result in amplicons of significantly different sizes for each species. By significantly different is meant that the sizes differ by at least 15 bp. This difference in size ensures that an agarose gel can be used to separate the amplicons by electrophoresis. An agarose gel has the advantage of not being influenced by the sequence of the amplicons and therefore it is insensitive to polymorphisms within the amplicons. It differentiates only by by size. In comparison polyacrylamide gels are sensitive to to sequence variation such as polymorphisms which may affect the resolution of the electrophoresis on an polyacrylamide gel. So the separation in a polyacrylamide gel depends on both the nature and the length of the sequence.
Multiplex-PCR may be useful in identifying the presence of different Astigmata mite species in a sample, such as in environmental samples as well as in certifying the purity and lack of cross-contamination in a single species culture.
In some embodiments, the method may be preceded by a preamplification step. This is advantageous if the sample has a low content of rDNA such as in environmental samples.
In a second aspect, the present invention relates to an isolated nucleic acid molecule at least about 80% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NOs:1-100 or fragment thereof, or complementary sequence thereof.
In some embodiments, the nucleic acid molecule is a polynucleotide.
These sequences provide new sequence information which is useful in designing new primers or probes for the identification, detection, discrimination or differentiation of different mite species in a sample. Also the sequence information provided confirms the phylogenetic relationship of the Astigmata mites identified.
Whenever used herein and in some embodiments, the phrase “at least about 80% identical to” refers to a sequence of at least about 81% identical to, such as at least about 82% identical to, such as at least about 83% identical to, such as at least about 84% identical to, such as at least about 85% identical to, such as at least about 86% identical to, such as at least about 87% identical to, such as at least about 88% identical to, such as at least about 89% identical to, such as at least about 90% identical to, such as at least about 91% identical to, such as at least about 92% identical to, such as at least about 93% identical to, such as at least about 94% identical to, such as at least about 95% identical to, such as at least about 96% identical to, such as at least about 97% identical to, such as at least about 98% identical to, such as at least about 99% identical to, such as about 100% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NO:1-100 or fragment thereof, or complementary sequence thereof.
In some embodiments, the isolated nucleic acid molecule is at least about 80% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NO:1-100 or fragment thereof.
In some embodiments, the isolated nucleic acid molecule is at least about 80% identical to a complementary sequence of a nucleic acid sequence selected from the list consisting of SEQ ID NO:1-100 or fragment thereof.
In a further aspect, the present invention relates to a composition comprising nucleic acid molecules of one or more, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 different species in the Astigmata suborder, the nucleic acid molecules being at least about 80% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NO:1-100 or fragment thereof, or complementary sequence thereof.
In some embodiments, the composition according to the present invention comprises nucleic acid molecules of at least 2, such as at least 3, such as at least 4, such as at least 5, such as at least 6, such as at least 7, such as at least 8, such as at least 9, such as 10 different species in the Astigmata suborder at least about 80% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NO:1-100 or fragment thereof, or complementary sequence thereof.
In some embodiments, the composition according to the present invention comprises sequences to detect, discriminate, or identify two or more, such as 2, 3, 4, 5, 6, 7, 8, 9, or 10 different species selected from the list consisting of Tyrophagus fanetzhangorum, Lepidoglyphus destructor, Glycyphagus domesticus, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Blomia tropicalis, Euroglyphus maynei, Dermatophagoides micro ceras, Acarus siro, and Dermatophagoides farinae.
In some embodiments, the composition according to the present invention further comprises a nucleic acid molecule at least about 80% identical to 5.8S in a sequence selected from any one of SEQ ID NOs:1-100, or the complementary sequence thereof, or fragment thereof, such as Rast5.8, such as a nucleic acid sequence defined by SEQ ID NO:111, or the complementary sequence thereof.
Accordingly, the composition may in one embodiment comprise first and second primers designed on the ITS1 sequence of the Astigmata mite species to be identified. In a specific embodiment, the first primers are designed on the ITS1 sequence and the second primer(s) is/are designed on the 5.8S sequence. Such composition has the advantage that the number of different primers used may be reduced if several Astigmata mite species are to be identified in a single assay. As will be clear to the skilled person the total amount of forward primers must equal the total amount of revers primers.
In a further aspect, the present invention relates to the use of one or more nucleic acid molecules at least about 80% identical to a nucleic acid sequence independently selected from the list consisting of SEQ ID NOs:1-111 or fragment thereof, or complementary sequence thereof, for the detection, discrimination, or identification of one or more, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 different specific species of the Astigmata suborder. In some embodiments, the one or more nucleic acid molecule is/are a nucleic acid molecule according to the present invention. In some embodiments, the nucleic acid molecule is as defined herein, or is part of a composition according to present invention.
In some embodiments, the isolated nucleic acid molecule is as defined herein and comprising ITS1, to design a primer which is unique to a specific Astigmata mite species. In some embodiments, the use is of an isolated nucleic acid molecule as defined herein and comprising 5.8S or 18S to design a primer which specifically hybridises to any of the rDNA of the Astigmata mite species of Tyrophagus fanetzhangorum, Lepidoglyphus destructor, Glycyphagus domesticus, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Blomia tropicalis, Euroglyphus maynei, Dermatophagoides microceras, Acarus siro and Dermatophagoides farinae.
In order to design a species specific primer, based on the sequence of ITS1, to be useful for species identification, the following steps may be performed:
1. Align all know sequences of ITS1 from Astigmata mite species. Given the intra-individual and the intra-specific polymorphism, it is recommendable to include in the analysis more than one sequences from each species that could represent natural polymorphism.
2. Select from the alignment the regions full-filling two requisites: high intra-specific conservation and low inter-specific conservation (this regions can be defined as “species specific”)
3. Select, among the regions in point 2, those containing sequences that could be appropriate for the design of primers following the standard roles for primer design (e.g. 18-32 consecutive nucleotides containing more than 40% of G/C against A/T; no self-complementariness, etc.)
4. Design primers having a relative high Tm (for example, using the “bases stacking method”, Tm could be between 52 and 56), between 18 and 30 bps (18-23 recommendable) and a good quality considering GC composition, complexity (polyX and triplet repetitions), 3′ stability and self dimers (The software AmplifX v1.4.4 ([Nicolas Jullien 2001-2007] or any other software may be use for primer design.
5. Once the primers are designed, select the primers not showing a high similarity (mainly at their 3′ end) to known sequences of other organisms (the analysis may be performed by BLASTN against public databases). The primers selected at this point would be good candidates for PCRs, however, selection must continue in order to select the primers that could be suitable for a PCR.
Species specific direct sense primers should be combined with an appropriate reverse sense primer that should be based on conserved regions of the rDNA, preferably it should be an Astigmata-specific primer. Thus, primers should be selected to:
i. show no complementariness with the reverse primer or primers to be used in the PCR reaction.
ii. show no complementariness with the other primers to be used in a multiplex PCR
Finally, the combinations of primers forward-reverse in a PCR should be designed to obtain amplicons of different size when amplifying DNA from different species.
In a further aspect, the present invention relates to amplicons obtained by the method according to the invention.
In a further aspect, the present invention relates to a molecular size marker composition for use in the method according to the invention comprising one or more polynucleotides, such as DNA of a size (in base pairs) corresponding to one or more amplicons obtained by the method according to the invention. A size corresponding to the size of the amplicons means the exact sizes of the amplicons +−30, 20 or 10 base pairs.
Such composition may be useful when comparing the size of the amplicon of the mite to be detected with the molecular markers. When the reference nucleotide has nearly the same size as the amplicon to be evaluated, it is easier to compare with the eye and thus to identify the species.
In a further aspect, the present invention relates to a method for the identification of one or more different Astigmata mite species in a sample, the method comprising the steps of:
a) Obtaining DNA from the sample;
b) Amplifying a region of the rDNA of the each of the mite species to be identified using
-
- i. a first primer, each primer specifically hybridising to the ITS1 sequence of the rDNA of each of the mite species to be identified, or the complementary sequence thereof, and
- ii. a second primer specifically hybridising to a sequence selected from 18S, 5.8S and 28S sequences of the rDNA of the mite species to be identified, or the complementary sequence thereof,
to produce an amplicon specific to the mite species to be identified, and;
c) identifying the mite species by evaluating the molecular size of the amplicon.
In some embodiments, the method according to the present invention is performed using one or more sets of a forward and a reverse primers, wherein at least one of said primers of a set is specific for said species and identical to a sequence at least about 80% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NOs:1-100 or fragment thereof, or complementary sequence thereof.
In some embodiments, the method according to the present invention is performed with primers of a composition according to the present invention.
In some embodiments, the method according to the present invention further comprises a step after step a) of amplification, such as by PCR, of any rDNA component in said sample, such as by use of primer pairs specific to 18S, 5.8S or 28S sequences.
Such preamplification may be useful if the samples have a low content of rDNA material to be identified such as when only a few or even only one mite is present, for instance in environmental samples.
In a further aspect, the present invention relates to a kit of parts comprising:
-
- a) A composition according to the invention; and
- b) A molecular size marker, such as a molecular size marker composition as defined herein.
In some embodiments, the kit comprises a pair of primers specific to 18S, 5.8S or 28S sequences suitable for amplification, such as by PCR, of any rDNA component in a sample. In some embodiments, the kit further comprises an extraction solution and/or an instruction manual.
In a further aspect, the present invention relates to a method for the preparation of a certified specimen of an Astigmata mite culture or of a purified fraction thereof, wherein the identity of one or more specific species in the Astigmata suborder in said sample is known, the method comprising the steps of
a) Obtaining DNA from a sample of the culture or purified fraction;
b) Detecting a nucleic acid molecule specific for said species, said sequence being identical to a nucleic acid sequence at least about 80% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NOs:1-100 or fragment thereof, or complementary sequence thereof;
c) Identifying said specific species in the Astigmata suborder based on the detection of a nucleic acid molecule specific for said species;
d) Obtaining said specimen, wherein the identity of one or more specific species in the Astigmata suborder in said specimen is known from step c).
In some embodiments, step b) is performed using PCR on the rDNA with one or more set of a forward and a reverse primer, wherein at least one of said primers of a set is specific for said species and identical to a sequence at least about 80% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NOs:1-100 or fragment thereof, or complementary sequence thereof.
In some embodiments, the PCR is performed with primers of a composition as defined herein.
In some embodiments, step b) is preceded by a preamplification step, such as by PCR, wherein the rDNA of all Astigmata mite species in the sample is amplified using a first primer specifically hybridising to the 18S sequence of the rDNA and a second primer specifically hybridising to a sequence selected from the 5.8S or 28S sequences of the rDNA.
In some embodiments, the one or more specific species in the Astigmata suborder is selected from the list consisting of: Tyrophagus fanetzhangorum, Lepidoglyphus destructor, Glycyphagus domesticus, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Blomia tropicalis, Euroglyphus maynei, Dermatophagoides microceras, Acarus siro and Dermatophagoides farinae.
In a further aspect, the present invention relates to a mite culture or a purified fraction prepared according to this method, such as a preparation of a certified mite culture or of a certified purified fraction.
Instead of or as a supplement to using species-specific ITS1 derived first primers in step b) i., it is equally possible to perform a molecular amplification which includes the presence of a detectable probe, which has the same hybridization characteristics as the above-defined first primer (i.e. that it hybridises specifically with a part of the ITS1 sequence (or its complementary sequence) of one single species of Astigmata; obviously, the probe must have a nucleic acid sequence that matches part of the amplicon obtained. Such a probe is particularly useful in embodiments of qPCR or real-time PCR, where a signal from the specific probe can be detected/recorded after conclusion of each amplification cycle, as is well-known in the art. Such a probe can e.g. be in the form of a nucleic acid sequence equipped with a fluorescent probe and a matching quencher, where the quencher is released when the probe is incorporated into an amplicon by a DNA polymerase.
Therefore, only probes that ultimately are present in an amplicon will fluoresce, providing a precise quantitative measure of the amount of amplicon from each cycle by methods well-known in the art. Particularly high specificity can be obtained if both the first primer and such a probe fulfil the hybridisation requirements for a first primer defined herein—but as mentioned it will be possible to use a more generic primer as a first primer, if a specific probe is included. In embodiments where several species are to be determined, the fluorescent probes used to detect each species can each be uniquely labelled so as to fluoresce at different wavelengths; hence, in multiplex amplifications, the relative quantities of different amplicons can be determined by correlating to the relative fluorescence intensities at the relevant wavelengths.
LEGENDS TO THE FIGURES FIG. 1. One step Multiplex-PCR analysis of DNA extracted from mites cultures provided by ALK-ABELLÓ. Each lane is from left: M (100 bp DNA Ladder (Promega)). Ma: (Marker adapted for identification of allergy-causing mites), T. fanetzhangorum (Tf), Lepidoglyphus destructor (Ld), Glycyphagusdomesticus (Gd), D. pteronyssinus (Dp), Tyrophagus putrescentiae (Tp), Ma, Blomia tropicalis (Bt), Euroglyphus maynei (Em), Dermatophagoides microceras (Dm), Acarus siro (As), D. farinae (Df), Ma, and M. (see Example 4).
FIG. 2. Two steps Multiplex-PCR analysis of DNA extracted from mites cultures provided by ALK-ABELLÓ. Each lane is from left: M (100 bp DNA Ladder (Promega)). Ma: (Marker adapted for identification of allergy-causing mites), T. fanetzhangorum (Tf), Lepidoglyphus destructor (Ld), Glycyphagusdomesticus (Gd), D. pteronyssinus (Dp), Tyrophagus putrescentiae (Tp), Ma, Blomia tropicalis (Bt), Euroglyphus maynei (Em), Dermatophagoides microceras (Dm), Acarus siro (As), D. farinae (Df), Ma, and M. (see Example 4).
FIG. 3. Ma Marker. DNA ladder prepared from nucleotides of by sizes corresponding to the amplicons produced in Example 2 for Tyrophagus fanetzhangorum (Tf), Lepidoglyphus destructor (Ld), Glycyphagus domesticus (Gd), Dermatophagoides pteronyssinus (Dp), Tyrophagus putrescentiae (Tp), Blomia tropicalis (Bt), Euroglyphus maynei (Em), Dermatophagoides microceras (Dm), Acarus siro (As), Dermatophagoides farinae (Df).
FIG. 4. Representation of the primers of Example 2 and Example 3, step 3
FIG. 5. Representation of the primers of Example 3, step 2 (preamplification)
FIG. 6. One step Multiplex-PCR analysis of DNA extracted from mite cultures provided by ALK-ABELLÓ. Each lane is from left: 100 bp DNA Ladder (Promega), T. fanetzhangorum (Tf), Lepidoglyphus destructor (Ld), Glycyphagusdomesticus (Gd), D. pteronyssinus (Dp), Tyrophagus putrescentiae (Tp), Blomia tropicalis (Bt), Euroglyphus maynei (Em), Dermatophagoides microceras (Dm), Acarus siro (As), D. farinae (Df), and 100 bp DNA Ladder (Promega).
DETAILED DISCLOSURE OF THE INVENTION Definitions When terms such as “one”, “a” or “an” are used in this disclosure they mean “at least one”, or “one or more” unless otherwise indicated. Further, the term “comprising” is intended to mean “including” and thus allows for the presence of other constituents, features, conditions, or steps than those explicitly recited.
The term “purified fraction” of a mass reared culture refers to a fraction of the culture, which is of mite origin, for instance mite bodies (body fraction) or mite faeces (faeces fraction). The purified fractions may be obtained from a mite culture by any fractionation method, such as by sieving or otherwise separating the sample. The predominant content of the purified fraction is bodies or faeces of one or more specific mite species compared to other constituents of the culture, such a nutrients and waste products.
The term “identification” as used herein refers to the mere detection or determination of the presence of one or more specific Astigmata mite species in a sample, the identification of the specific Astigmata mite species, as well as the ability to discriminate between one or more different specific Astigmata mite species in a sample. For example, identification of a mite species can refer to determining which phylogenetic genus, species, or subspecies an individual mite belongs.
The term “Ribosomal DNA” or “rDNA”, as used herein refers to a DNA sequence that codes for ribosomal RNA, such as the ribosomal RNA of Astigmata mite species. Ribosomes are assemblies of proteins and rRNA molecules that translate mRNA molecules to produce proteins. rDNA of eukaryotes including mites consists of a tandem repeat of a unit segment, an operon, containing the elements 18S, ITS1, 5.8S, ITS2, and 28S.
The term “Internal transcribed spacer 1 (ITS1)” or ITS1 as used herein refers to the nucleic acid sequence, such as in any one of SEQ ID NOs:1-100 situated between the nucleic acid sequences encoding the structural ribosomal RNAs 18S rRNA and 5.8S rRNA. Accordingly, ITS1 is defined by having boundaries to 18S (5′ AGGATCATTA 3′) and to 5.8S (5′, CTGYYAGTGG 3′).
The term “Internal transcribed spacer 2 (ITS2)” or ITS2 as used herein refers to the nucleic acid sequence, such as in any one of SEQ ID NOs:1-100 situated between the nucleic acid sequences encoding the structural ribosomal RNAs 5.8S rRNA and 28S rRNA. Accordingly, ITS2 is defined by having boundaries to 5.8S (5′ TGAGCGTCGT 3′) and to 28S (5′ CGACCTCAG 3′).
The term “5.8S” as used herein refers to the nucleic acid sequence, such as in any one of SEQ ID NOs:1-100 situated between ITS1 and ITS2, such as the nucleic acid sequences encoding the structural ribosomal RNAs with boundaries 5′, CTGYYAGTGG 3′ and 5′ TGAGCGTCGT 3′ of SEQ ID NO:1-100.
The term “28S” as used herein refers to the nucleic acid sequence encoding the structural ribosomal 28S RNAs just downstream of ITS2 having boundaries (5′ CGACCTCAG 3′) of SEQ ID NO:1-100.
The term “18S” as used herein refers to the nucleic acid sequence encoding the structural ribosomal 18S RNAs just upstream of ITS1 having boundaries (5′ AGGATCATTA 3′) of SEQ ID NO:1-100.
As used herein the term “first primer” refers to a primer in a set of primers used in the amplification, such as by PCR, of a rDNA fragment. The first primer may be the forward primer or the reverse primer relative to the “second primer”. It follows that the term “second primer” also refers to a primer in a set of primers used in the amplification, such as by PCR, of a ribosomal DNA
An “isolated” molecule is a molecule that is the predominant species in the composition wherein it is found with respect to the class of molecules to which it belongs (i.e. it makes up at least about 50% of the type of molecule in the composition and typically will make up at least about 70%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, or more of the species of molecule, e.g., nucleotide or peptide, in the composition). Commonly, a composition of a nucleic acid molecule will exhibit 98%-99% homogeneity for nucleic acid molecules in the context of all present nucleic acid species in the composition or at least with respect to substantially active nucleic acid species in the context of the proposed use.
The term “specifically hybridising to” refers to primers or probes which, under suitable conditions, specifically hybridise with the relevant nucleic acids. Said suitable conditions are preferably stringent hybridisation conditions as defined below. In a preferred embodiment, a probe hybridises only with one nucleic acid, e.g. a rDNA for one particular mite species clone. For example, a primer that “specifically hybridizes” to an ITS1 sequence or a “specific primer” describes a primer that hybridizes to only one mite species in a sample of multiple mite species. Likewise, an amplicon “specific for” a given mite species describes an amplicon that is present (or amplified from) only one mite species to be identified hi a sample comprising multiple mite species. Alternatively, it is preferred that a probe hybridises with several nucleic acid clones of the same type of mite species.
“Stringent hybridisation conditions” include conditions comprising e.g.: overnight incubation at 65° C. in 4×SSC (600 mM sodium chloride, 60 mM sodium citrate), followed by a washing step at 65° C. in 0.1×SSC for 1 hour. Alternatively, it is possible to incubate at 42° C. in a solution that contains 50% formamide, 5×SSC (750 mM sodium chloride, 75 mM sodium citrate), 50 mM sodium phosphate (pH 7.6), 5×Denhardt's solution, 10% dextrane sulphate and 20 μg/ml denatured, sheared DNA from salmon sperm, followed by washing steps in 0.1×SSC of 5 to 20 minutes at approximately 65° C. These hybridisation conditions are known to the person skilled in the art as highly stringent hybridisation conditions. Unless otherwise stated, the term “Sequence identity” for nucleotides as used herein refers to the sequence identity calculated as 100−(nref−ndif)·100/nref, wherein ndif is the total number of non-identical nucleotides in the two sequences when aligned and wherein nref is the number of residues in one of the sequences.
Unless otherwise stated, the number of residues nref and the alignment are made only in the length of the shortest sequence. Accordingly, if a short primer is compared with the sequence of a longer DNA sequence, only the sequence of the overlap or corresponding regions thereof is compared. Hence, the nucleic acid sequence GCATACCGTGTTGAAGCAGG will have a sequence identity of 80% with the sequence AAATACCGTGTTGAAGCAAA (ndif=4 and nref=20). The alignment may be be done direct-direct or direct reverse. The alignment showing the maximum similarity should be used.
In some embodiments, the sequence identity is determined by conventional methods, e.g., Smith and Waterman, 1981, Adv. Appl. Math. 2:482, by the search for similarity method of Pearson & Lipman, 1988, Proc. Natl. Acad. Sci. USA 85:2444, using the CLUSTAL W algorithm of Thompson et al., 1994, Nucleic Acids Res 22:467380, by computerized implementations of these algorithms (BLASTN, BLASTX and TBLASTX, GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group). The BLAST algorithm (Altschul et al., 1990, Mol. Biol. 215:403-10) for which software may be obtained through the National Center for Biotechnology Information www.ncbi.nlm.nih.gov/) may also be used. When using any of the aforementioned algorithms, the default parameters for “Window” length, gap penalty, etc., are used.
Sequence identity analysis includes database search and alignment. Examples of public databases include the DNA Database of Japan (DDBJ) (on the World Wide Web at ddbj.nig.acjp/); Genebank (on the World Wide Web at ncbi.nlm.nih.gov/Web/Search/Index.htlm); and the European Molecular Biology Laboratory Nucleic Acid Sequence Database (EMBL) (on the World Wide Web at ebi.ac.uk/ebi_docs/embl_db/embl-db.html). Other appropriate databases include dbEST (on the World Wide Web at ncbi.nlm.nih.gov/dbEST/index.html), Swissprot (on the World Wide Web at ebi.ac.uk/ebi_docs/swisprot db/swisshome.html), PIR (on the World Wide Web at nbrt.georgetown.edu/pir/) and The Institute for Genome Research (on the World Wide Web at tigr.org/tdb/tdb.html).
A number of different search algorithms have been developed, one example of which are the suite of programmes referred to as BLAST programmes. There are five implementations of BLAST, three designed for nucleotide sequences queries (BLASTN, BLASTX and TBLASTX) and two designed for protein sequence queries (BLASTP and TBLASTN) (Coulson, Trends in Biotechnology 12:76-80 (1994); Birren et al., Genome Analysis 1, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 543-559 (1997)).
BLASTN takes a nucleotide sequence (the query sequence) and its reverse complement and searches them against a nucleotide sequence database. BLASTN was designed for speed, not maximum sensitivity and may not find distantly related coding sequences. BLASTX takes a nucleotide sequence, translates it in three forward reading frames and three reverse complement reading frames and then compares the six translations against a protein sequence database. BLASTX is useful for sensitive analysis of preliminary (single-pass) sequence data and is tolerant of sequencing errors (Gish and States, Nature Genetics 3:266-272 (1993), the entirety of which is herein incorporated by reference). BLASTN and BLASTX may be used in concert for analyzing EST data (Coulson, Trends in Biotechnology 12:76-80 (1994); Birren et al., Genome Analysis 1:543-559 (1997)).
Given a coding nucleotide sequence and the protein it encodes, it is often preferable to use the protein as the query sequence to search a database because of the greatly increased sensitivity to detect more subtle relationships. This is due to the larger alphabet of proteins (20 amino acids) compared with the alphabet of nucleic acid sequences (4 bases), where it is far easier to obtain a match by chance. In addition, with nucleotide alignments, only a match (positive score) or a mismatch (negative score) is obtained, but with proteins, the presence of conservative amino acid substitutions can be taken into account. Here, a mismatch may yield a positive score if the non-identical residue has physical/chemical properties similar to the one it replaced. Various scoring matrices are used to supply the substitution scores of all possible amino acid pairs. A general purpose scoring system is the BLOSUM62 matrix (Henikoff and Henikoff, Proteins 17:49-61 (1993), the entirety of which is herein incorporated by reference), which is currently the default choice for BLAST programmes. BLOSUM62 is tailored for alignments of moderately diverged sequences and thus may not yield the best results under all conditions. Altschul, J. Mol. Biol. 36:290-300 (1993), the entirety of which is herein incorporated by reference, describes a combination of three matrices to cover all contingencies. This may improve sensitivity, but at the expense of slower searches. In practice, a single BLOSUM62 matrix is often used but others (PAM40 and PAM250) may be attempted when additional analysis is necessary. Low PAM matrices are directed at detecting very strong but localized sequence similarities, whereas high PAM matrices are directed at detecting long but weak alignments between very distantly related sequences.
Homologues in other organisms are available that can be used for comparative sequence analysis. Multiple alignments are performed to study similarities and differences in a group of related sequences. CLUSTAL W is a multiple sequence alignment package that performs progressive multiple sequence alignments based on the method of Feng and Doolittle, J. Mol. Evol. 25:351-360 (1987), the entirety of which is herein incorporated by reference. Each pair of sequences is aligned and the distance between each pair is calculated; from this distance matrix, a guide tree is calculated and all of the sequences are progressively aligned based on this tree. A feature of the program is its sensitivity to the effect of gaps on the alignment; gap penalties are varied to encourage the insertion of gaps in probable loop regions instead of in the middle of structured regions. Users can specify gap penalties, choose between a number of scoring matrices, or supply their own scoring matrix for both pairwise alignments and multiple alignments. CLUSTAL W for UNIX and VMS systems is available at: ftb.ebi.ac.uk. Another program is MACAW (Schuler et al., Proteins Struct. Func. Genet. 9:180-190 (1991), the entirety of which is herein incorporated by reference, for which both Macintosh and Microsoft Windows versions are available. MACAW uses a graphical interface, provides a choice of several alignment algorithms and is available by anonymous ftp at: ncbi.nlm.nih.gov (directory/pub/macaw).
SPECIFIC EMBODIMENTS OF THE INVENTION As described above the present invention relates to a method for the identification of one or more different Astigmata mite species in a sample, the method comprising the steps of:
a) obtaining DNA from the sample;
b) amplifying, such as by PCR, a region of the rDNA of each of the mite species to be identified using
-
- i. one or more, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 first primers specifically hybridising to the ITS1 sequence of the rDNA of the mite species to be identified, or the complementary sequence thereof, and
- ii. one or more, such as one, second primers specifically hybridising to a sequence selected from any of the 18S, 5.8S or 28S sequences of the rDNA of the mite species to be identified, or the complementary sequence thereof,
to produce an amplicon specific to the mite species to be identified, and;
c) identifying the mite species by evaluating a characteristic of the amplicon.
In some embodiments under step b), the amplicon produced has a molecular size which is characteristic of the specific mite species to be identified.
In some embodiments under step c), the mite species is identified by evaluating the molecular size of the amplicon which is characteristic of the mite species to be identified.
However, the amplicons may also be characterised by sequencing the amplicon and identifying the mite species by comparing to SEQ ID NO's:1-100.
In some embodiments, less than 13, such as 10, such as 8, such as 6, such as 5, such as 3 different Astigmata mites are identified.
In some embodiments under step b), two or more amplicons specific to the mite species to be identified are produced, which amplicons differ in length by at least 15 bp, such as 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 bp.
In some embodiments, the second primer is 90%, such as 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% identical to at least 15 consecutive nucleotides of said sequence of any of the Astigmata mite species to be identified.
In some embodiments, the one or more first primers used in step b) i. contains at least 3, such as 4, 5 or 6 consecutive nucleotides in the 3′ end with exact complementarity to any ITS1 sequence of the mite species to be identified.
In some embodiments, the one or more first primers used in step b) i. is at least about 70%, such as 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99% identical to the sequence of any corresponding part of the ITS1 sequence or a complementary part thereof of the mite species to be identified.
In some embodiments, the method is for the identification of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, or more different Astigmata mite species in the sample.
In some embodiments, step c) is performed by comparing the molecular size(s) of the amplicon(s) to the molecular sizes of reference nucleotides of a molecular marker composition, the sizes of the reference nucleotides spanning the relevant base pair interval.
Reference nucleotide compositions are commercially available. An example is the Thermo Scientific GeneRuler 100 bp DNA Ladder. It contains reference nucleotides of 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100 bp. It is suitable for both agarose gels and polyacrylamide gels. Another DNA ladder is available from Promega. The ladder is dissolved in buffer and electroforesed together with the DNA sample to be analysed. When reading amplicon sizes using such a classic DNA ladder, the amplicon size is conveniently estimated by comparing by eye the distance travelled by the amplicon with the distance travelled by the reference nucleotides of the ladder (having steps of 100 bp).
In some embodiments, the sizes of the reference nucleotides correspond to the sizes of the amplicons characteristic of the mite species to be identified. An advantage of using such reference nucleotides of the sizes of the amplicons to be identified, is that it becomes easier to compare the sizes of the amplicons with the sizes of the reference nucleotides. Especially by eye.
Electrophoresing a reference nucleotide composition together with the sample on a gel enables identification of each Astigmata mite species present in the sample directly from the result of the electrophoresis by comparing the sample result with the reference nucleotide composition. No intermediate step is necessary, such as sequencing the amplicon or evaluating the band pattern of multiple amplicons per mite species to be identified.
In some embodiments, step b) is preceded by a preamplification step, such as by PCR, wherein the rDNA containing the ITS1 region of all Astigmata mite species in the sample is amplified using a first primer specifically hybridising to the 18S sequence of the rDNA and a second primer specifically hybridising to a sequence selected from the 5.8S and 28S sequences of the rDNA.
In some embodiments, the sample is an environmental sample.
In some embodiments, the sample is from a mass reared culture or a purified fraction thereof.
In some embodiments, the sample is from a mass reared culture or a purified fraction thereof wherein a preamplification step according to claim 10 is not conducted.
In some embodiments, two or more first primers are used, each primer specifically hybridising to the ITS1 sequences of one mite species to be identified, or the complementary sequence thereof, and not cross hybridising to other mite species to be identified.
In some embodiments, the first primer is designed on two or more, such as 2, 3, 4, 5, 6, 7, 8, 9, or 10 groups of sequences identified by any one of SEQ ID NOs:1-10, SEQ ID NOs:11-20, SEQ ID NOs:21-30, SEQ ID NOs:31-40, SEQ ID NOs:41-50, SEQ ID NOs:51-60, SEQ ID NOs:61-70, SEQ ID NOs:71-80, SEQ ID NOs:81-90, and SEQ ID NOs:91-10.
In some embodiments, the first primer referred to in b) i. comprises a sequence at least about 70%, such as 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% identical to the ITS1 of a sequence selected from any one of SEQ ID NOs:1-100, or the complementary sequence thereof, or a fragment thereof.
In some embodiments, the first primer is at least about 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 contiguous nucleotides in length.
In some embodiments, the first primer is not more than about 70, 60, 50, 40, 30, 25, 23, 20 contiguous nucleotides in length.
In some embodiments, the first primer comprises a sequence at least about 70%, such as 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NO:101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 122, 123, and 124, or the complementary sequence thereof, or fragment thereof, or complementary sequence thereof.
In some embodiments, the first primer consists of a sequence at least about 70%, such as 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NO:101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 122, 123, and 124, or the complementary sequence thereof, or fragment thereof.
In some embodiments, the second primer, comprises a nucleic acid sequence at least about 70%, such as 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% identical to a fragment of 5.8S in a sequence selected from any one of SEQ ID NOs:1-100, or the complementary sequence thereof, such as Rast5.8, such as a nucleic acid sequence defined by SEQ ID NO:111 or the complementary sequence thereof, or fragment thereof.
In some embodiments, the second primer, comprises a nucleic acid sequence at least about 70%, such as 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% identical to a fragment of 18S in a sequence selected from any one of SEQ ID NOs:1-100, or the complementary sequence thereof, such as FRibNav, such as a nucleic acid sequence defined by SEQ ID NO:121 or the complementary sequence thereof, or fragment thereof.
In some embodiments, the one or more different species in the Astigmata suborder is/are selected from the group consisting of: Tyrophagus fanetzhangorum, Lepidoglyphus destructor, Glycyphagus domesticus, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Blomia tropicalis, Euroglyphus maynei, Dermatophagoides microceras, Acarus siro and Dermatophagoides farinae.
As mentioned above, the present invention relates to an isolated nucleic acid molecule at least about 80% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NOs:1-100 or fragment thereof, or complementary sequence thereof.
In some embodiments, the isolated nucleic acid molecule is at least about 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 contiguous nucleotides in length.
In some embodiments, the isolated nucleic acid molecule according to the invention is at least 7, such as at least 8, such as at least 9, such as at least 10, such as at least 11, such as at least 12, such as at least 13, such as at least 14, such as at least 15, such as at least 16, such as at least 17, such as at least 18, such as at least 19, such as at least 20, such as at least 21, such as at least 22, such as at least 23, such as at least 24, such as at least 25, such as at least 26, such as at least 27, such as at least 28, such as at least 29, such as at least 30, such as at least 31, such as at least 32, such as at least 33, such as at least 34, such as at least 35, such as at least 36, such as at least 37, such as at least 38, such as at least 39, such as at least 40, such as at least 41, such as at least 42, such as at least 43, such as at least 44, such as at least 45, such as at least 46, such as at least 47, such as at least 48, such as at least 49, such as at least 50, such as at least 51, such as at least 52, such as at least 53, such as at least 54, such as at least 55, such as at least 56, such as at least 57, such as at least 58, such as at least 59, such as at least 60, such as at least 61, such as at least 62, such as at least 63, such as at least 64, such as at least 65, such as at least 66, such as at least 67, such as at least 68, such as at least 69, such as at least 70, such as at least 71, such as at least 72, such as at least 73, such as at least 74, such as at least 75, such as at least 76, such as at least 77, such as at least 78, such as at least 79, such as at least 80, such as at least 81, such as at least 82, such as at least 83, such as at least 84, such as at least 85, such as at least 86, such as at least 87, such as at least 88, such as at least 89, such as at least 90, such as at least 91, such as at least 92, such as at least 93, such as at least 94, such as at least 95, such as at least 96, such as at least 97, such as at least 98, such as at least 99, such as at least 100, such as at least 101, such as at least 102, such as at least 103, such as at least 104, such as at least 105, such as at least 106, such as at least 107, such as at least 108, such as at least 109, such as at least 110, such as at least 111, such as at least 112, such as at least 113, such as at least 114, such as at least 115, such as at least 116, such as at least 117, such as at least 118, such as at least 119, such as at least 120, such as at least 121, such as at least 122, such as at least 123, such as at least 124, such as at least 125, such as at least 126, such as at least 127, such as at least 128, such as at least 129, such as at least 130, such as at least 131, such as at least 132, such as at least 133, such as at least 134, such as at least 135, such as at least 136, such as at least 137, such as at least 138, such as at least 139, such as at least 140, such as at least 141, such as at least 142, such as at least 143, such as at least 144, such as at least 145, such as at least 146, such as at least 147, such as at least 148, such as at least 149, such as at least 150, such as at least 151, such as at least 152, such as at least 153, such as at least 154, such as at least 155, such as at least 156, such as at least 157, such as at least 158, such as at least 159, such as at least 160, such as at least 161, such as at least 162, such as at least 163, such as at least 164, such as at least 165, such as at least 166, such as at least 167, such as at least 168, such as at least 169, such as at least 170, such as at least 171, such as at least 172, such as at least 173, such as at least 174, such as at least 175, such as at least 176, such as at least 177, such as at least 178, such as at least 179, such as at least 180, such as at least 181, such as at least 182, such as at least 183, such as at least 184, such as at least 185, such as at least 186, such as at least 187, such as at least 188, such as at least 189, such as at least 190, such as at least 191, such as at least 192, such as at least 193, such as at least 194, such as at least 195, such as at least 196, such as at least 197, such as at least 198, such as at least 199, such as at least 200, such as at least 201, such as at least 202, such as at least 203, such as at least 204, such as at least 205, such as at least 206, such as at least 207, such as at least 208, such as at least 209, such as at least 210, such as at least 211, such as at least 212, such as at least 213, such as at least 214, such as at least 215, such as at least 216, such as at least 217, such as at least 218, such as at least 219, such as at least 220, such as at least 221, such as at least 222, such as at least 223, such as at least 224, such as at least 225, such as at least 226, such as at least 227, such as at least 228, such as at least 229, such as at least 230, such as at least 231, such as at least 232, such as at least 233, such as at least 234, such as at least 235, such as at least 236, such as at least 237, such as at least 238, such as at least 239, such as at least 240, such as at least 241, such as at least 242, such as at least 243, such as at least 244, such as at least 245, such as at least 246, such as at least 247, such as at least 248, such as at least 249, such as at least 250, such as at least 251, such as at least 252, such as at least 253, such as at least 254, such as at least 255, such as at least 256, such as at least 257, such as at least 258, such as at least 259, such as at least 260, such as at least 261, such as at least 262, such as at least 263, such as at least 264, such as at least 265, such as at least 266, such as at least 267, such as at least 268, such as at least 269, such as at least 270, such as at least 271, such as at least 272, such as at least 273, such as at least 274, such as at least 275, such as at least 276, such as at least 277, such as at least 278, such as at least 279, such as at least 280, such as at least 281, such as at least 282, such as at least 283, such as at least 284, such as at least 285, such as at least 286, such as at least 287, such as at least 288, such as at least 289, such as at least 290, such as at least 291, such as at least 292, such as at least 293, such as at least 294, such as at least 295, such as at least 296, such as at least 297, such as at least 298, such as at least 299, such as at least 300, such as at least 301, such as at least 302, such as at least 303, such as at least 304, such as at least 305, such as at least 306, such as at least 307, such as at least 308, such as at least 309, such as at least 310, such as at least 311, such as at least 312, such as at least 313, such as at least 314, such as at least 315, such as at least 316, such as at least 317, such as at least 318, such as at least 319, such as at least 320, such as at least 321, such as at least 322, such as at least 323, such as at least 324, such as at least 325, such as at least 326, such as at least 327, such as at least 328, such as at least 329, such as at least 330, such as at least 331, such as at least 332, such as at least 333, such as at least 334, such as at least 335, such as at least 336, such as at least 337, such as at least 338, such as at least 339, such as at least 340, such as at least 341, such as at least 342, such as at least 343, such as at least 344, such as at least 345, such as at least 346, such as at least 347, such as at least 348, such as at least 349, such as at least 350, such as at least 351, such as at least 352, such as at least 353, such as at least 354, such as at least 355, such as at least 356, such as at least 357, such as at least 358, such as at least 359, such as at least 360, such as at least 361, such as at least 362, such as at least 363, such as at least 364, such as at least 365, such as at least 366, such as at least 367, such as at least 368, such as at least 369, such as at least 370, such as at least 371, such as at least 372, such as at least 373, such as at least 374, such as at least 375, such as at least 376, such as at least 377, such as at least 378, such as at least 379, such as at least 380, such as at least 381, such as at least 382, such as at least 383, such as at least 384, such as at least 385, such as at least 386, such as at least 387, such as at least 388, such as at least 389, such as at least 390, such as at least 391, such as at least 392, such as at least 393, such as at least 394, such as at least 395, such as at least 396, such as at least 397, such as at least 398, such as at least 399, such as at least 400, such as at least 401, such as at least 402, such as at least 403, such as at least 404, such as at least 405, such as at least 406, such as at least 407, such as at least 408, such as at least 409, such as at least 410, such as at least 411, such as at least 412, such as at least 413, such as at least 414, such as at least 415, such as at least 416, such as at least 417, such as at least 418, such as at least 419, such as at least 420, such as at least 421, such as at least 422, such as at least 423, such as at least 424, such as at least 425, such as at least 426, such as at least 427, such as at least 428, such as at least 429, such as at least 430, such as at least 431, such as at least 432, such as at least 433, such as at least 434, such as at least 435, such as at least 436, such as at least 437, such as at least 438, such as at least 439, such as at least 440, such as at least 441, such as at least 442, such as at least 443, such as at least 444, such as at least 445, such as at least 446, such as at least 447, such as at least 448, such as at least 449, such as at least 450, such as at least 451, such as at least 452, such as at least 453, such as at least 454, such as at least 455, such as at least 456, such as at least 457, such as at least 458, such as at least 459, such as at least 460, such as at least 461, such as at least 462, such as at least 463, such as at least 464, such as at least 465, such as at least 466, such as at least 467, such as at least 468, such as at least 469, such as at least 470, such as at least 471, such as at least 472, such as at least 473, such as at least 474, such as at least 475, such as at least 476, such as at least 477, such as at least 478, such as at least 479, such as at least 480, such as at least 481, such as at least 482, such as at least 483, such as at least 484, such as at least 485, such as at least 486, such as at least 487, such as at least 488, such as at least 489, such as at least 490, such as at least 491, such as at least 492, such as at least 493, such as at least 494, such as at least 495, such as at least 496, such as at least 497, such as at least 498, such as at least 499, such as at least 500, such as at least 501, such as at least 502, such as at least 503, such as at least 504, such as at least 505, such as at least 506, such as at least 507, such as at least 508, such as at least 509, such as at least 510, such as at least 511, such as at least 512, such as at least 513, such as at least 514, such as at least 515, such as at least 516, such as at least 517, such as at least 518, such as at least 519, such as at least 520, such as at least 521, such as at least 522, such as at least 523, such as at least 524, such as at least 525, such as at least 526, such as at least 527, such as at least 528, such as at least 529, such as at least 530, such as at least 531, such as at least 532, such as at least 533, such as at least 534, such as at least 535, such as at least 536, such as at least 537, such as at least 538, such as at least 539, such as at least 540, such as at least 541, such as at least 542, such as at least 543, such as at least 544, such as at least 545, such as at least 546, such as at least 547, such as at least 548, such as at least 549, such as at least 550, such as at least 551, such as at least 552, such as at least 553, such as at least 554, such as at least 555, such as at least 556, such as at least 557, such as at least 558, such as at least 559, such as at least 560, such as at least 561, such as at least 562, such as at least 563, such as at least 564, such as at least 565, such as at least 566, such as at least 567, such as at least 568, such as at least 569, such as at least 570, such as at least 571, such as at least 572, such as at least 573, such as at least 574, such as at least 575, such as at least 576, such as at least 577, such as at least 578, such as at least 579, such as at least 580, such as at least 581, such as at least 582, such as at least 583, such as at least 584, such as at least 585, such as at least 586, such as at least 587, such as at least 588, such as at least 589, such as at least 590, such as at least 591 contiguous nucleotides in length.
In some embodiments, the isolated nucleic acid molecule according to the invention is not more than 999 contiguous nucleotides, such as not more than 998, such as not more than 997, such as not more than 996, such as not more than 995, such as not more than 994, such as not more than 993, such as not more than 992, such as not more than 991, such as not more than 990, such as not more than 989, such as not more than 988, such as not more than 987, such as not more than 986, such as not more than 985, such as not more than 984, such as not more than 983, such as not more than 982, such as not more than 981, such as not more than 980, such as not more than 979, such as not more than 978, such as not more than 977, such as not more than 976, such as not more than 975, such as not more than 974, such as not more than 973, such as not more than 972, such as not more than 971, such as not more than 970, such as not more than 969, such as not more than 968, such as not more than 967, such as not more than 966, such as not more than 965, such as not more than 964, such as not more than 963, such as not more than 962, such as not more than 961, such as not more than 960, such as not more than 959, such as not more than 958, such as not more than 957, such as not more than 956, such as not more than 955, such as not more than 954, such as not more than 953, such as not more than 952, such as not more than 951, such as not more than 950, such as not more than 949, such as not more than 948, such as not more than 947, such as not more than 946, such as not more than 945, such as not more than 944, such as not more than 943, such as not more than 942, such as not more than 941, such as not more than 940, such as not more than 939, such as not more than 938, such as not more than 937, such as not more than 936, such as not more than 935, such as not more than 934, such as not more than 933, such as not more than 932, such as not more than 931, such as not more than 930, such as not more than 929, such as not more than 928, such as not more than 927, such as not more than 926, such as not more than 925, such as not more than 924, such as not more than 923, such as not more than 922, such as not more than 921, such as not more than 920, such as not more than 919, such as not more than 918, such as not more than 917, such as not more than 916, such as not more than 915, such as not more than 914, such as not more than 913, such as not more than 912, such as not more than 911, such as not more than 910, such as not more than 909, such as not more than 908, such as not more than 907, such as not more than 906, such as not more than 905, such as not more than 904, such as not more than 903, such as not more than 902, such as not more than 901, such as not more than 900, such as not more than 899, such as not more than 898, such as not more than 897, such as not more than 896, such as not more than 895, such as not more than 894, such as not more than 893, such as not more than 892, such as not more than 891, such as not more than 890, such as not more than 889, such as not more than 888, such as not more than 887, such as not more than 886, such as not more than 885, such as not more than 884, such as not more than 883, such as not more than 882, such as not more than 881, such as not more than 880, such as not more than 879, such as not more than 878, such as not more than 877, such as not more than 876, such as not more than 875, such as not more than 874, such as not more than 873, such as not more than 872, such as not more than 871, such as not more than 870, such as not more than 869, such as not more than 868, such as not more than 867, such as not more than 866, such as not more than 865, such as not more than 864, such as not more than 863, such as not more than 862, such as not more than 861, such as not more than 860, such as not more than 859, such as not more than 858, such as not more than 857, such as not more than 856, such as not more than 855, such as not more than 854, such as not more than 853, such as not more than 852, such as not more than 851, such as not more than 850, such as not more than 849, such as not more than 848, such as not more than 847, such as not more than 846, such as not more than 845, such as not more than 844, such as not more than 843, such as not more than 842, such as not more than 841, such as not more than 840, such as not more than 839, such as not more than 838, such as not more than 837, such as not more than 836, such as not more than 835, such as not more than 834, such as not more than 833, such as not more than 832, such as not more than 831, such as not more than 830, such as not more than 829, such as not more than 828, such as not more than 827, such as not more than 826, such as not more than 825, such as not more than 824, such as not more than 823, such as not more than 822, such as not more than 821, such as not more than 820, such as not more than 819, such as not more than 818, such as not more than 817, such as not more than 816, such as not more than 815, such as not more than 814, such as not more than 813, such as not more than 812, such as not more than 811, such as not more than 810, such as not more than 809, such as not more than 808, such as not more than 807, such as not more than 806, such as not more than 805, such as not more than 804, such as not more than 803, such as not more than 802, such as not more than 801, such as not more than 800, such as not more than 799, such as not more than 798, such as not more than 797, such as not more than 796, such as not more than 795, such as not more than 794, such as not more than 793, such as not more than 792, such as not more than 791, such as not more than 790, such as not more than 789, such as not more than 788, such as not more than 787, such as not more than 786, such as not more than 785, such as not more than 784, such as not more than 783, such as not more than 782, such as not more than 781, such as not more than 780, such as not more than 779, such as not more than 778, such as not more than 777, such as not more than 776, such as not more than 775, such as not more than 774, such as not more than 773, such as not more than 772, such as not more than 771, such as not more than 770, such as not more than 769, such as not more than 768, such as not more than 767, such as not more than 766, such as not more than 765, such as not more than 764, such as not more than 763, such as not more than 762, such as not more than 761, such as not more than 760, such as not more than 759, such as not more than 758, such as not more than 757, such as not more than 756, such as not more than 755, such as not more than 754, such as not more than 753, such as not more than 752, such as not more than 751, such as not more than 750, such as not more than 749, such as not more than 748, such as not more than 747, such as not more than 746, such as not more than 745, such as not more than 744, such as not more than 743, such as not more than 742, such as not more than 741, such as not more than 740, such as not more than 739, such as not more than 738, such as not more than 737, such as not more than 736, such as not more than 735, such as not more than 734, such as not more than 733, such as not more than 732, such as not more than 731, such as not more than 730, such as not more than 729, such as not more than 728, such as not more than 727, such as not more than 726, such as not more than 725, such as not more than 724, such as not more than 723, such as not more than 722, such as not more than 721, such as not more than 720, such as not more than 719, such as not more than 718, such as not more than 717, such as not more than 716, such as not more than 715, such as not more than 714, such as not more than 713, such as not more than 712, such as not more than 711, such as not more than 710, such as not more than 709, such as not more than 708, such as not more than 707, such as not more than 706, such as not more than 705, such as not more than 704, such as not more than 703, such as not more than 702, such as not more than 701, such as not more than 700, such as not more than 699, such as not more than 698, such as not more than 697, such as not more than 696, such as not more than 695, such as not more than 694, such as not more than 693, such as not more than 692, such as not more than 691, such as not more than 690, such as not more than 689, such as not more than 688, such as not more than 687, such as not more than 686, such as not more than 685, such as not more than 684, such as not more than 683, such as not more than 682, such as not more than 681, such as not more than 680, such as not more than 679, such as not more than 678, such as not more than 677, such as not more than 676, such as not more than 675, such as not more than 674, such as not more than 673, such as not more than 672, such as not more than 671, such as not more than 670, such as not more than 669, such as not more than 668, such as not more than 667, such as not more than 666, such as not more than 665, such as not more than 664, such as not more than 663, such as not more than 662, such as not more than 661, such as not more than 660, such as not more than 659, such as not more than 658, such as not more than 657, such as not more than 656, such as not more than 655, such as not more than 654, such as not more than 653, such as not more than 652, such as not more than 651, such as not more than 650, such as not more than 649, such as not more than 648, such as not more than 647, such as not more than 646, such as not more than 645, such as not more than 644, such as not more than 643, such as not more than 642, such as not more than 641, such as not more than 640, such as not more than 639, such as not more than 638, such as not more than 637, such as not more than 636, such as not more than 635, such as not more than 634, such as not more than 633, such as not more than 632, such as not more than 631, such as not more than 630, such as not more than 629, such as not more than 628, such as not more than 627, such as not more than 626, such as not more than 625, such as not more than 624, such as not more than 623, such as not more than 622, such as not more than 621, such as not more than 620, such as not more than 619, such as not more than 618, such as not more than 617, such as not more than 616, such as not more than 615, such as not more than 614, such as not more than 613, such as not more than 612, such as not more than 611, such as not more than 610, such as not more than 609, such as not more than 608, such as not more than 607, such as not more than 606, such as not more than 605, such as not more than 604, such as not more than 603, such as not more than 602, such as not more than 601, such as not more than 600, such as not more than 599, such as not more than 598, such as not more than 597, such as not more than 596, such as not more than 595, such as not more than 594, such as not more than 593, such as not more than 592, such as not more than 591, such as not more than 590, such as not more than 589, such as not more than 588, such as not more than 587, such as not more than 586, such as not more than 585, such as not more than 584, such as not more than 583, such as not more than 582, such as not more than 581, such as not more than 580, such as not more than 579, such as not more than 578, such as not more than 577, such as not more than 576, such as not more than 575, such as not more than 574, such as not more than 573, such as not more than 572, such as not more than 571, such as not more than 570, such as not more than 569, such as not more than 568, such as not more than 567, such as not more than 566, such as not more than 565, such as not more than 564, such as not more than 563, such as not more than 562, such as not more than 561, such as not more than 560, such as not more than 559, such as not more than 558, such as not more than 557, such as not more than 556, such as not more than 555, such as not more than 554, such as not more than 553, such as not more than 552, such as not more than 551, such as not more than 550, such as not more than 549, such as not more than 548, such as not more than 547, such as not more than 546, such as not more than 545, such as not more than 544, such as not more than 543, such as not more than 542, such as not more than 541, such as not more than 540, such as not more than 539, such as not more than 538, such as not more than 537, such as not more than 536, such as not more than 535, such as not more than 534, such as not more than 533, such as not more than 532, such as not more than 531, such as not more than 530, such as not more than 529, such as not more than 528, such as not more than 527, such as not more than 526, such as not more than 525, such as not more than 524, such as not more than 523, such as not more than 522, such as not more than 521, such as not more than 520, such as not more than 519, such as not more than 518, such as not more than 517, such as not more than 516, such as not more than 515, such as not more than 514, such as not more than 513, such as not more than 512, such as not more than 511, such as not more than 510, such as not more than 509, such as not more than 508, such as not more than 507, such as not more than 506, such as not more than 505, such as not more than 504, such as not more than 503, such as not more than 502, such as not more than 501, such as not more than 500, such as not more than 499, such as not more than 498, such as not more than 497, such as not more than 496, such as not more than 495, such as not more than 494, such as not more than 493, such as not more than 492, such as not more than 491, such as not more than 490, such as not more than 489, such as not more than 488, such as not more than 487, such as not more than 486, such as not more than 485, such as not more than 484, such as not more than 483, such as not more than 482, such as not more than 481, such as not more than 480, such as not more than 479, such as not more than 478, such as not more than 477, such as not more than 476, such as not more than 475, such as not more than 474, such as not more than 473, such as not more than 472, such as not more than 471, such as not more than 470, such as not more than 469, such as not more than 468, such as not more than 467, such as not more than 466, such as not more than 465, such as not more than 464, such as not more than 463, such as not more than 462, such as not more than 461, such as not more than 460, such as not more than 459, such as not more than 458, such as not more than 457, such as not more than 456, such as not more than 455, such as not more than 454, such as not more than 453, such as not more than 452, such as not more than 451, such as not more than 450, such as not more than 449, such as not more than 448, such as not more than 447, such as not more than 446, such as not more than 445, such as not more than 444, such as not more than 443, such as not more than 442, such as not more than 441, such as not more than 440, such as not more than 439, such as not more than 438, such as not more than 437, such as not more than 436, such as not more than 435, such as not more than 434, such as not more than 433, such as not more than 432, such as not more than 431, such as not more than 430, such as not more than 429, such as not more than 428, such as not more than 427, such as not more than 426, such as not more than 425, such as not more than 424, such as not more than 423, such as not more than 422, such as not more than 421, such as not more than 420, such as not more than 419, such as not more than 418, such as not more than 417, such as not more than 416, such as not more than 415, such as not more than 414, such as not more than 413, such as not more than 412, such as not more than 411, such as not more than 410, such as not more than 409, such as not more than 408, such as not more than 407, such as not more than 406, such as not more than 405, such as not more than 404, such as not more than 403, such as not more than 402, such as not more than 401, such as not more than 400, such as not more than 399, such as not more than 398, such as not more than 397, such as not more than 396, such as not more than 395, such as not more than 394, such as not more than 393, such as not more than 392, such as not more than 391, such as not more than 390, such as not more than 389, such as not more than 388, such as not more than 387, such as not more than 386, such as not more than 385, such as not more than 384, such as not more than 383, such as not more than 382, such as not more than 381, such as not more than 380, such as not more than 379, such as not more than 378, such as not more than 377, such as not more than 376, such as not more than 375, such as not more than 374, such as not more than 373, such as not more than 372, such as not more than 371, such as not more than 370, such as not more than 369, such as not more than 368, such as not more than 367, such as not more than 366, such as not more than 365, such as not more than 364, such as not more than 363, such as not more than 362, such as not more than 361, such as not more than 360, such as not more than 359, such as not more than 358, such as not more than 357, such as not more than 356, such as not more than 355, such as not more than 354, such as not more than 353, such as not more than 352, such as not more than 351, such as not more than 350, such as not more than 349, such as not more than 348, such as not more than 347, such as not more than 346, such as not more than 345, such as not more than 344, such as not more than 343, such as not more than 342, such as not more than 341, such as not more than 340, such as not more than 339, such as not more than 338, such as not more than 337, such as not more than 336, such as not more than 335, such as not more than 334, such as not more than 333, such as not more than 332, such as not more than 331, such as not more than 330, such as not more than 329, such as not more than 328, such as not more than 327, such as not more than 326, such as not more than 325, such as not more than 324, such as not more than 323, such as not more than 322, such as not more than 321, such as not more than 320, such as not more than 319, such as not more than 318, such as not more than 317, such as not more than 316, such as not more than 315, such as not more than 314, such as not more than 313, such as not more than 312, such as not more than 311, such as not more than 310, such as not more than 309, such as not more than 308, such as not more than 307, such as not more than 306, such as not more than 305, such as not more than 304, such as not more than 303, such as not more than 302, such as not more than 301, such as not more than 300, such as not more than 299, such as not more than 298, such as not more than 297, such as not more than 296, such as not more than 295, such as not more than 294, such as not more than 293, such as not more than 292, such as not more than 291, such as not more than 290, such as not more than 289, such as not more than 288, such as not more than 287, such as not more than 286, such as not more than 285, such as not more than 284, such as not more than 283, such as not more than 282, such as not more than 281, such as not more than 280, such as not more than 279, such as not more than 278, such as not more than 277, such as not more than 276, such as not more than 275, such as not more than 274, such as not more than 273, such as not more than 272, such as not more than 271, such as not more than 270, such as not more than 269, such as not more than 268, such as not more than 267, such as not more than 266, such as not more than 265, such as not more than 264, such as not more than 263, such as not more than 262, such as not more than 261, such as not more than 260, such as not more than 259, such as not more than 258, such as not more than 257, such as not more than 256, such as not more than 255, such as not more than 254, such as not more than 253, such as not more than 252, such as not more than 251, such as not more than 250, such as not more than 249, such as not more than 248, such as not more than 247, such as not more than 246, such as not more than 245, such as not more than 244, such as not more than 243, such as not more than 242, such as not more than 241, such as not more than 240, such as not more than 239, such as not more than 238, such as not more than 237, such as not more than 236, such as not more than 235, such as not more than 234, such as not more than 233, such as not more than 232, such as not more than 231, such as not more than 230, such as not more than 229, such as not more than 228, such as not more than 227, such as not more than 226, such as not more than 225, such as not more than 224, such as not more than 223, such as not more than 222, such as not more than 221, such as not more than 220, such as not more than 219, such as not more than 218, such as not more than 217, such as not more than 216, such as not more than 215, such as not more than 214, such as not more than 213, such as not more than 212, such as not more than 211, such as not more than 210, such as not more than 209, such as not more than 208, such as not more than 207, such as not more than 206, such as not more than 205, such as not more than 204, such as not more than 203, such as not more than 202, such as not more than 201, such as not more than 200, such as not more than 199, such as not more than 198, such as not more than 197, such as not more than 196, such as not more than 195, such as not more than 194, such as not more than 193, such as not more than 192, such as not more than 191, such as not more than 190, such as not more than 189, such as not more than 188, such as not more than 187, such as not more than 186, such as not more than 185, such as not more than 184, such as not more than 183, such as not more than 182, such as not more than 181, such as not more than 180, such as not more than 179, such as not more than 178, such as not more than 177, such as not more than 176, such as not more than 175, such as not more than 174, such as not more than 173, such as not more than 172, such as not more than 171, such as not more than 170, such as not more than 169, such as not more than 168, such as not more than 167, such as not more than 166, such as not more than 165, such as not more than 164, such as not more than 163, such as not more than 162, such as not more than 161, such as not more than 160, such as not more than 159, such as not more than 158, such as not more than 157, such as not more than 156, such as not more than 155, such as not more than 154, such as not more than 153, such as not more than 152, such as not more than 151, such as not more than 150, such as not more than 149, such as not more than 148, such as not more than 147, such as not more than 146, such as not more than 145, such as not more than 144, such as not more than 143, such as not more than 142, such as not more than 141, such as not more than 140, such as not more than 139, such as not more than 138, such as not more than 137, such as not more than 136, such as not more than 135, such as not more than 134, such as not more than 133, such as not more than 132, such as not more than 131, such as not more than 130, such as not more than 129, such as not more than 128, such as not more than 127, such as not more than 126, such as not more than 125, such as not more than 124, such as not more than 123, such as not more than 122, such as not more than 121, such as not more than 120, such as not more than 119, such as not more than 118, such as not more than 117, such as not more than 116, such as not more than 115, such as not more than 114, such as not more than 113, such as not more than 112, such as not more than 111, such as not more than 110, such as not more than 109, such as not more than 108, such as not more than 107, such as not more than 106, such as not more than 105, such as not more than 104, such as not more than 103, such as not more than 102, such as not more than 101, such as not more than 100, such as not more than 99, such as not more than 98, such as not more than 97, such as not more than 96, such as not more than 95, such as not more than 94, such as not more than 93, such as not more than 92, such as not more than 91, such as not more than 90, such as not more than 89, such as not more than 88, such as not more than 87, such as not more than 86, such as not more than 85, such as not more than 84, such as not more than 83, such as not more than 82, such as not more than 81, such as not more than 80, such as not more than 79, such as not more than 78, such as not more than 77, such as not more than 76, such as not more than 75, such as not more than 74, such as not more than 73, such as not more than 72, such as not more than 71, such as not more than 70, such as not more than 69, such as not more than 68, such as not more than 67, such as not more than 66, such as not more than 65, such as not more than 64, such as not more than 63, such as not more than 62, such as not more than 61, such as not more than 60, such as not more than 59, such as not more than 58, such as not more than 57, such as not more than 56, such as not more than 55, such as not more than 54, such as not more than 53, such as not more than 52, such as not more than 51, such as not more than 50, such as not more than 49, such as not more than 48, such as not more than 47, such as not more than 46, such as not more than 45, such as not more than 44, such as not more than 43, such as not more than 42, such as not more than 41, such as not more than 40, such as not more than 39, such as not more than 38, such as not more than 37, such as not more than 36, such as not more than 35, such as not more than 34, such as not more than 33, such as not more than 32, such as not more than 31, such as not more than 30, such as not more than 29, such as not more than 28, such as not more than 27, such as not more than 26, such as not more than 25 contiguous nucleotides in length.
In some embodiments, the isolated nucleic acid molecule is not more than about 1200, 1100, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 90, 80, 70, 60, 50, 40, 30, or 20 contiguous nucleotides in length.
In some embodiments, the isolated nucleic acid molecule is specific for Tyrophagus fanetzhangorum. In some embodiments, the isolated nucleic acid molecule is specific for Lepidoglyphus destructor. In some embodiments, the isolated nucleic acid molecule is specific for Glycyphagus domesticus. In some embodiments, the isolated nucleic acid molecule is specific for Dermatophagoides pteronyssinus. In some embodiments, the isolated nucleic acid molecule is specific for Tyrophagus putrescentiae. In some embodiments, the isolated nucleic acid molecule is specific for Blomia tropicalis. In some embodiments, the isolated nucleic acid molecule is specific for Euroglyphus maynei. In some embodiments, the isolated nucleic acid molecule is specific for Dermatophagoides microceras. In some embodiments, the isolated nucleic acid molecule is specific for Acarus siro. In some embodiments, the isolated nucleic acid molecule is specific for Dermatophagoides farinae.
In some embodiments, the isolated nucleic acid molecule comprises a sequence at least about 80% identical to the internal transcribed spacer 1 (ITS1) of a sequence selected from any one of SEQ ID NOs:1-100, or the complementary sequence thereof, or fragment thereof.
In some embodiments, the isolated nucleic acid molecule comprises a sequence at least about 80% identical to the internal transcribed spacer 2 (ITS2) of a sequence selected from any one of SEQ ID NOs:1-100, or the complementary sequence thereof, or fragment thereof.
In some embodiments, the isolated nucleic acid molecule comprises a sequence at least about 80% identical to the internal transcribed spacer 1 (ITS1) and internal transcribed spacer 2 (ITS2) of the same sequence selected from any one of SEQ ID NOs:1-100, or the complementary sequence thereof, or fragment thereof.
In some embodiments the isolated nucleic acid molecule is comprising a sequence at least about 80% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NO:101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 122, 123, and 124, or the complementary sequence thereof, or fragment thereof, or complementary sequence thereof.
In some embodiments, the isolated nucleic acid molecule is consisting of a sequence at least about 80% identical to a nucleic acid sequence selected from the list consisting of SEQ ID NO:101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 122, 123, and 124, or the complementary sequence thereof, or fragment thereof.
In some embodiments, the isolated nucleic acid molecule is comprising a nucleic acid sequence at least about 80% identical to 5.8S in a sequence selected from any one of SEQ ID NOs:1-100, or the complementary sequence thereof, such as Rast5.8, such as a nucleic acid sequence defined by SEQ ID NO:111 or the complementary sequence thereof, or fragment thereof.
In some embodiments, the isolated nucleic acid molecule is comprising a nucleic acid sequence at least about 80% identical to 18S in a sequence selected from any one of SEQ ID NOs:1-100, or the complementary sequence thereof, such as FRibNav, such as a nucleic acid sequence defined by SEQ ID NO:121 or the complementary sequence thereof, or fragment thereof.
Sequences:
Clon_DM1
(SEQ ID NO: 1)
Clon_DM21
(SEQ ID NO: 2)
Clon_DM6
(SEQ ID NO: 3)
Clon_DM20
(SEQ ID NO: 4)
Clon_DM9
(SEQ ID NO: 5)
Clon_DM12
(SEQ ID NO: 6)
Clon_DM7
(SEQ ID NO: 7)
Clon_DM11
(SEQ ID NO: 8)
Clon_DM14
(SEQ ID NO: 9)
Clon_DMA
(SEQ ID NO: 10)
Clon_DF1
(SEQ ID NO: 11)
Clon_DF6
(SEQ ID NO: 12)
Clon_DF4
(SEQ ID NO: 13)
Clon_DF26
(SEQ ID NO: 14)
Clon_DF4_50
(SEQ ID NO: 15)
Clon_DF19
(SEQ ID NO: 16)
Clon_DF3
(SEQ ID NO: 17)
Clon_DF5
(SEQ ID NO: 18)
Clon_DF2
(SEQ ID NO: 19)
Clon_DF7
(SEQ ID NO: 20)
Clon_AS15
(SEQ ID NO: 21)
Clon_AS14
(SEQ ID NO: 22)
Clon_AS20
(SEQ ID NO: 23)
Clon_AS13
(SEQ ID NO: 24)
Clon_AS10
(SEQ ID NO: 25)
Clon_AS11
(SEQ ID NO: 26)
Clon_AS2
(SEQ ID NO: 27)
Clon_AS12
(SEQ ID NO: 28)
Clon_AS1
(SEQ ID NO: 29)
Clon_AS16
(SEQ ID NO: 30)
Clon_BT8
(SEQ ID NO: 31)
Clon_BT9
(SEQ ID NO: 32)
Clon_BT16
(SEQ ID NO: 33)
Clon_BT3
(SEQ ID NO: 34)
Clon_BT14
(SEQ ID NO: 35)
Clon_BT17
(SEQ ID NO: 36)
Clon_BT13
(SEQ ID NO: 37)
Clon_BT1
(SEQ ID NO: 38)
Clon_BT10
(SEQ ID NO: 39)
Clon_BT15
(SEQ ID NO: 40)
Clon_TPA1_20
(SEQ ID NO: 41)
Clon_TPA1_22
(SEQ ID NO: 42)
Clon_TPA1_29
(SEQ ID NO: 43)
Clon_TPA1_28
(SEQ ID NO: 44)
Clon_TPA1_26
(SEQ ID NO: 45)
Clon_TPA1_21
(SEQ ID NO: 46)
Clon_TPA1_36
(SEQ ID NO: 47)
Clon_TPA1_27
(SEQ ID NO: 48)
Clon_TPA1_23
(SEQ ID NO: 49)
Clon_TPA1_1
(SEQ ID NO: 50)
Clon_TF22
(SEQ ID NO: 51)
Clon_TF24
(SEQ ID NO: 52)
Clon_TF3
(SEQ ID NO: 53)
Clon_TF2
(SEQ ID NO: 54)
Clon_TF23
(SEQ ID NO: 55)
Clon_TF1
(SEQ ID NO: 56)
Clon_TF4
(SEQ ID NO: 57)
Clon_TF7
(SEQ ID NO: 58)
Clon_TF15
(SEQ ID NO: 59)
Clon_TF14
(SEQ ID NO: 60)
Clon_DP8
(SEQ ID NO: 61)
Clon_DP1
(SEQ ID NO: 62)
Clon_DP7
(SEQ ID NO: 63)
Clon_DP3
(SEQ ID NO: 64)
Clon_DP6
(SEQ ID NO: 65)
Clon_DP9
(SEQ ID NO: 66)
Clon_DP2
(SEQ ID NO: 67)
Clon_DP4
(SEQ ID NO: 68)
Clon_DP10
(SEQ ID NO: 69)
Clon_DP5
(SEQ ID NO: 70)
Clon_EM4
(SEQ ID NO: 71)
Clon_EM21
(SEQ ID NO: 72)
Clon_EM2
(SEQ ID NO: 73)
Clon_EM23
(SEQ ID NO: 74)
Clon_EM3
(SEQ ID NO: 75)
Clon_EM24
(SEQ ID NO: 76)
Clon_EM22
(SEQ ID NO: 77)
Clon_EM1
(SEQ ID NO: 78)
Clon_EM6
(SEQ ID NO: 79)
Clon_EM5
(SEQ ID NO: 80)
Clon_GD1
(SEQ ID NO: 81)
Clon_GD10
(SEQ ID NO: 82)
Clon_GD2
(SEQ ID NO: 83)
Clon_GD5
(SEQ ID NO: 84)
Clon_GD3
(SEQ ID NO: 85)
Clon_GD12
(SEQ ID NO: 86)
Clon_GD7
(SEQ ID NO: 87)
Clon_GD9
(SEQ ID NO: 88)
Clon_GD8
(SEQ ID NO: 89)
Clon_GD13
(SEQ ID NO: 90)
Clon_LD5
(SEQ ID NO: 91)
Clon_LD13
(SEQ ID NO: 92)
Clon_LD14
(SEQ ID NO: 93)
Clon_LD1
(SEQ ID NO: 94)
Clon_LD11
(SEQ ID NO: 95)
Clon_LD3
(SEQ ID NO: 96)
Clon_LD2
(SEQ ID NO: 97)
Clon_LD12
(SEQ ID NO: 98)
Clon_LD8
(SEQ ID NO: 99)
Clon_LD15
(SEQ ID NO: 100)
Primer Sequences (5′-3′):
Forward (first) primers:
F1Tf_824
(SEQ ID NO: 101)
GACAGAAGCTGAAAGCCGT (Tyrophagus fanetzhangorum)
F1Ld_608
(SEQ ID NO: 102)
GATGTTCGAATCAATTGCTAGTG (Lepidoglyphus destructor)
F1Gd_567
(SEQ ID NO: 103)
GCATACCGTGTTGAAGCAGG (Glycyphagus domesticus)
F1Dp_501
(SEQ ID NO: 104)
GATCGACTGGCAATTGTTGAC
(Dermatophagoides pteronyssinus)
F1Tp_474
(SEQ ID NO: 105)
CGCCATTTGACACAGTACC (Tyrophagus putrescentiae)
F1Bt_419
(SEQ ID NO: 106)
TGTGTGTGGGGGATTTTGC (Blomia tropicalis)
F1Em_384
(SEQ ID NO: 107)
GAGCCTGACAATTATCAATGTGC (Euroglyphus maynei)
F1Dm_304
(SEQ ID NO: 108)
CGGGATGAACGTGTGGATG (Dermatophagoides microceras)
F1As_234
(SEQ ID NO: 109)
GTCGGTTACGGTCAAACG (Acarus siro)
F1Df_159
(SEQ ID NO: 110)
GAAACAATTGAATTGTGATTCTGC (Dermatophagoides farinae)
Reverse universal (second) primer:
RAst5.85
(SEQ ID NO: 111)
5′-TGCGTTCGAAWGTCGAGT-3′, W = T or A
Forward universal (second) primer:
FRibNav
(SEQ ID NO: 121)
5′-AGAGGAAGTAAAAGTCGTAACAAG-3′
Reverse (first) primers:
R1Dp_181
(SEQ ID NO: 122)
GCTTTCAATAACCTCATCAGTGTC (Dermatophagoides
pteronyssinus)
R1Bt_347
(SEQ ID NO: 123)
CCATCACTAAAGGACAGAACCGC (Blomia tropicalis)
R1Df_419
(SEQ ID NO: 124)
CTCCAGCAATCGAATTATGCTC (Dermatophagoides farinae)
Sequences and CLUSTAL W 2.1 multiple sequence alignment.
ITS1 and ITS2 are defined herein by the boundaries of ITS1 and ITS2 to the conserved sequences of 18s (in bold), 5.8s (2nd sequence in bold), and 28s (3rd sequence in bold). Accordingly, ITS1 is defined by the sequences having 18s with the sequence 5′-AGGATCATTA-3′ in the 5′ terminal of ITS1, and 5.8s with the sequence 5′-CTGYYAGTGG-3′ in the 3′ terminal of ITS1 (the sequences of 18s and 5.8s not included). ITS2 is defined by the sequences having 5.8s with the sequence 5′ TGAGCGTCGT 3′ in the 5′ terminal of ITS2, and 28s with the sequence 5′ CGACCTCAG 3′ in the 3′ terminal of ITS2 (the sequences of 5.8s and 28s not included). ITS1 goes downstream 18S sub-unit, and ITS2 goes downstream 5.8S sub-unit
Clon_DM1 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DM21 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DM6 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DM20 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DM9 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DM12 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DM7 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DM11 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DM14 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DMA GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DF1 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DF6 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DF4 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DF26 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DF450 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DF19 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DF3 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DF5 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DF2 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_DF7 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGG--TGTT-----------------
Clon_AS15 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATAGTTGCTTTGCT-TGCA----
Clon_AS14 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATAGTTGCTTTGCT-TGCA----
Clon_AS20 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATAGTTGCTTTGCT-TGCA----
Clon_AS13 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATAGTTGCTTTGCT-TGCA----
Clon_AS10 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATAGTTGCTTTGCT-TGCA----
Clon_AS11 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATAGTTGCTTTGCT-TGCA----
Clon_AS2 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATAGTTGCTTTGCT-TGCA----
Clon_AS12 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATAGTTGCTTTGCT-TGCA----
Clon_AS1 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATAGTTGCTTTGCT-TGCA----
Clon_AS16 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATAGTTGCTTTGCT-TGCA----
Clon_BT8 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATTGGATA-TTATTTTGGTGTG------
Clon_BT9 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATTGGATA-TTATTTTGGTGTG------
Clon_BT16 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATTGGATA-TTATTTTGGTGTG------
Clon_BT3 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATTGGATA-TTATTTTGGTGTG------
Clon_BT14 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATTGGATA-TTATTTTGGTGTG------
Clon_BT17 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATTGGATA-TTATTTTGGTGTG------
Clon_BT13 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATTGGATA-TTATTTTGGTGTG------
Clon_BT1 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATTGGATA-TTATTTTGGTGTG------
Clon_BT10 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATTGGATA-TTATTTTGGTGTG------
Clon_BT15 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATTGGATA-TTATTTTGGTGTG------
Clon_TPA1_20 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GAAGCGAAGGA-
Clon_TPA1_22 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GAAGCGAAGGA-
Clon_TPA1_29 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GAAGCGAAGGA-
Clon_TPA1_28 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GAAGCGAAGGA-
Clon_TPA1_26 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GAAGCGAAGGA-
Clon_TPA1_21 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GAAGCGAAGGA-
Clon_TPA1_36 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GAAGCGAAGGA-
Clon_TPA1_27 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GAAGCGAAGGA-
Clon_TPA1_23 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GAAGCGAAGGA-
Clon_TPA1_1 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GAAGCGAAGGA-
Clon_TF22 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GC--TGAAAGCC
Clon_TF24 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GC--TGAAAGCC
Clon_TF3 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GC--TGAAAGCC
Clon_TF2 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GC--TGAAAGCC
Clon_TF23 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GC--TGAAAGCC
Clon_TF1 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GC--TGAAAGCC
Clon_TF4 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GC--TGAAAGCC
Clon_TF7 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GC--TGAAAGCC
Clon_TF15 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GC--TGAAAGCC
Clon_TF14 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATCGACAGAA-GC--TGAAAGCC
Clon_DP8 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTCTTGAGCA-----------
Clon_DP1 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTCTTGAGCA-----------
Clon_DP7 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGCTTTCTTGAGCA-----------
Clon_DP3 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTCTTGAGCAA----------
Clon_DP6 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTCTTGAGCAA----------
Clon_DP9 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTCTTGAGCAA----------
Clon_DP2 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTCTTGAGCA-----------
Clon_DP4 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTCTTGAGCA-----------
Clon_DP10 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGATTTTCTTGAGCA-----------
Clon_DP5 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTCTTGAGCA-----------
Clon_EM4 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTTCGTGGCA-----------
Clon_EM21 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTTCGTGGCA-----------
Clon_EM2 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTTCGTGGCA-----------
Clon_EM23 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTTCGTGGCA-----------
Clon_EM3 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTTCGTGGCA-----------
Clon_EM24 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTTCGTGGCA-----------
Clon_EM22 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTTCGTGGCA-----------
Clon_EM1 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTTC--GACA-----------
Clon_EM6 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTATCGGTTTTTCGTGGCA-----------
Clon_EM5 GTTTCCGTAGGTGAACCTGCGGGAGGATCATTATCGGTTATTC--GACA-----------
Clon_GD1 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTGTC-------------
Clon_GD10 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTGTC-------------
Clon_GD2 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTGTC-------------
Clon_GD5 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTGTT-------------
Clon_GD3 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTGTC-------------
Clon_GD12 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTGTC-------------
Clon_GD7 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTGTT-------------
Clon_GD9 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTGTC-------------
Clon_GD8 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTGTC-------------
Clon_GD13 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTGTC-------------
Clon_LD5 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTATTCT-----------
Clon_LD13 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTATTCT-----------
Clon_LD14 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTATTCT-----------
Clon_LD1 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTATTCT-----------
ClonLD11 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTATTCT-----------
Clon_LD3 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTATTCT-----------
Clon_LD2 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTATTCT-----------
Clon_LD12 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTATTCT-----------
Clon_LD8 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTATTCT-----------
Clon_LD15 GTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGATTGTTTATTCT-----------
********************** ********** *
Clon_DM1 ----------------------------------------TTTTTT--------------
Clon_DM21 ----------------------------------------TTTTTT--------------
Clon_DM6 ----------------------------------------TTTTTT--------------
Clon_DM20 ----------------------------------------TTTTTT--------------
Clon_DM9 ----------------------------------------TTTTTT--------------
Clon_DM12 ----------------------------------------TTTTTT--------------
Clon_DM7 ----------------------------------------TTTTTT--------------
Clon_DM11 ----------------------------------------TTTTTT--------------
Clon_DM14 ----------------------------------------TTTTTT--------------
Clon_DMA ----------------------------------------TTTTTT--------------
Clon_DF1 ----------------------------------------TTT--T--------------
Clon_DF6 ----------------------------------------TTT--T--------------
Clon_DF4 ----------------------------------------TTTG-T--------------
Clon_DF26 ----------------------------------------TTTG-T--------------
Clon_DF4_50 ----------------------------------------TTT--T--------------
Clon_DF19 ----------------------------------------TTT--T--------------
Clon_DF3 ----------------------------------------TTTGTT--------------
Clon_DF5 ----------------------------------------TTTGTT--------------
Clon_DF2 ----------------------------------------TTTGTT--------------
Clon_DF7 ----------------------------------------TTTGTT--------------
Clon_AS15 -TTTGT----------------------------------TTTGCT--------------
Clon_AS14 -TTTGT----------------------------------TTTGCT--------------
Clon_AS20 -TTTGT----------------------------------TTTGCT--------------
Clon_AS13 -TTTGT----------------------------------TTTGCT--------------
Clon_AS10 -TTTGT----------------------------------TTTGCT--------------
Clon_AS11 -TTTACC---------------------------------TTTGCT--------------
Clon_AS2 -TTTAC----------------------------------TTTGCT--------------
Clon_AS12 -TTTACC---------------------------------TTTGCT--------------
Clon_AS1 -TTTAC----------------------------------TTTGCT--------------
Clon_AS16 -TTTAC----------------------------------TTTGCT--------------
Clon_BT8 -----------------------------------------TGATT--------------
Clon_BT9 -----------------------------------------TGATT--------------
Clon_BT16 -----------------------------------------TGATT--------------
Clon_BT3 -----------------------------------------TGATT--------------
Clon_BT14 -----------------------------------------TGATT--------------
Clon_BT17 -----------------------------------------TGATT--------------
Clon_BT13 -----------------------------------------TGATT--------------
Clon_BT1 -----------------------------------------TGATT--------------
Clon_BT10 -----------------------------------------TGATT--------------
Clon_BT15 -----------------------------------------TGATT--------------
Clon_TPA1_20 GTTCA-----G-------------------------CTCTTTCACT--------------
Clon_TPA1_22 GTTCA-----G-------------------------CTCTTTCACT--------------
Clon_TPA1_29 GTTCA-----G-------------------------CTCTTTCACT--------------
Clon_TPA1_28 GTTCA-----G-------------------------CTCTTTCACT--------------
Clon_TPA1_26 GTTCA-----G-------------------------CTCTTTCACT--------------
Clon_TPA1_21 GTTCA-----G-------------------------CTCTTTCACT--------------
Clon_TPA1_36 GTTCA-----G-------------------------CTCTTTCACT--------------
Clon_TPA1_27 GTTCA-----G-------------------------CTCTTTCACT--------------
Clon_TPA1_23 GTTCA-----G-------------------------CTCTTTCACT--------------
Clon_TPA1_1 GTTCA-----G-------------------------CTCTTTCACT--------------
Clon_TF22 GTCTGCTGTTGTGCTCTTGCAGTGCATCATCATCATCACTTTCACT--------------
Clon_TF24 GTCTGCTGTTGTGCTCTTGCAGTGCATCATCATCATCACTTTCACT--------------
Clon_TF3 GTCTGCTGTTGTGCTCTTGCAGTGCATCATCATCATCACTTTCACT--------------
Clon_TF2 GTCTGCTGTTGTGCTCTTGCAGTGCATCATCATCATCACTTTCACT--------------
Clon_TF23 GTCTGCTGTTGTGCTCTTGCAGTGCATCATCATCATCACTTTCACT--------------
Clon_TF1 GTCTGTTGTTGTGCTCTTGCAGTGCATCATCATTATCACTTTCACT--------------
Clon_TF4 GTCTGTTGTTGTGCTCTTGCAGTGCATCATCATTATCACTTTCACT--------------
Clon_TF7 GTCTGTTGTTGTGCTCTTGCAGTGCATCATCATTATCACTTTCACT--------------
Clon_TF15 GTCTGCTGTTGTGCTCTTGCAGTGCATCATCATCATCACTTTCACT--------------
Clon_TF14 GTTTGTTGTTGTGCTCTTGCGGTGCATCATCATTATCACTTTCACT--------------
Clon_DP8 ----------------------------------------TTCATTT-------------
Clon_DP1 ----------------------------------------TTCATTT-------------
Clon_DP7 ----------------------------------------TTCATTTT------------
Clon_DP3 ----------------------------------------TTTATTT-------------
Clon_DP6 ----------------------------------------TTTATTT-------------
Clon_DP9 ----------------------------------------TTTATTT-------------
Clon_DP2 ----------------------------------------TTTATTT-------------
Clon_DP4 ----------------------------------------TTTATTT-------------
Clon_DP10 ----------------------------------------TTCATTTT------------
Clon_DP5 ----------------------------------------TTTATTT-------------
Clon_EM4 ----------------------------------------TTCATTT-------------
Clon_EM21 ----------------------------------------TTCATTT-------------
Clon_EM2 ----------------------------------------TTCATTT-------------
Clon_EM23 ----------------------------------------TTCATTT-------------
Clon_EM3 ----------------------------------------TTCATTT-------------
Clon_EM24 ----------------------------------------TTCATTT-------------
Clon_EM22 -----------------------------------------TTCATTT------------
Clon_EM1 ----------------------------------------GT--TTT-------------
Clon_EM6 ----------------------------------------TTCATTT-------------
Clon_EM5 ----------------------------------------GT--TTT-------------
Clon_GD1 ----------------------------------------TTAATTTTTGCAAT-TTAC-
Clon_GD10 ----------------------------------------TTGATTTT--CAAT-TTATC
Clon_GD2 ----------------------------------------TTGATTTT--CAAT-TTATC
Clon_GD5 ----------------------------------------TTGATTTT--CAAT-TTATC
Clon_GD3 ----------------------------------------TTGATTTT--CAAT-TTATC
Clon_GD12 ----------------------------------------TTGATTTT--CAAT-TTATC
Clon_GD7 ----------------------------------------TTGATTTT--CAAT-TTATC
Clon_GD9 ----------------------------------------TTGATTTT--CAAT-TTATC
Clon_GD8 ----------------------------------------TTGATTTA--CAACGTTACA
Clon_GD13 ----------------------------------------TTGATTTA--CAACGTTACA
Clon_LD5 ----------------------------------------TTCATTGC----ATTTCACA
Clon_LD13 ----------------------------------------TTCATTGC----ATTTCACA
Clon_LD14 ----------------------------------------TTCATTGC----ATTTCACA
Clon_LD1 ----------------------------------------TTCATTGC----ATTTCACA
Clon_LD11 ----------------------------------------TTCATTGC----ATTTCACA
Clon_LD3 ----------------------------------------TTCATTGC----ATTTCACA
Clon_LD2 ----------------------------------------TTCATTGC----ATTTCACA
Clon_LD12 ----------------------------------------TTCATTGC----ATTTCACA
Clon_LD8 ----------------------------------------TTCATTGC----ATTTCA--
Clon_LD15 ----------------------------------------TTCATTGC----ATTTCA--
* *
Clon_DM1 ---G--------------------------CACTTGA----TT--CTTAAATCGAACGAA
Clon_DM21 ---G--------------------------CACTTGA----TT--CTTAAATCGAACGAA
Clon_DM6 ---G--------------------------CACTTGA----TT--CTTAAATCGAACGAA
Clon_DM20 ---G--------------------------CACTTGA----TT--CTTAAATCGAACGAA
Clon_DM9 --TG--------------------------CACTTGA----TT--CTTAAATCGAACGAA
Clon_DM12 --TG--------------------------CACTTGA----TT--CTTAAATCGAACGAA
Clon_DM7 ---G--------------------------CACTTGA----TT--CTTAAATCGAACGAA
Clon_DM11 --TG--------------------------CACTTGA----TT--CTTAAATCGAACGAA
Clon_DM14 --TG--------------------------CACTTGA----TT--CTTAAATCGAACGAA
Clon_DMA ---G--------------------------CACTTGA----TT--CTTAAATCGAACGAA
Clon_DF1 --TGAA-----CAATGAA-----------ACACTTGA----TT--CTCAATTCGAACGAT
Clon_DF6 --TGAA-----CAATGAA-----------ACACTTGA----TT--CTCAATTCGAACGAT
Clon_DF4 --TGAA-----CAATGAA-----------ACACTTGA----TT--CTCAATTCGAACGAT
Clon_DF26 --TGAA-----CAATGAA-----------ACACTTGA----TT--CTCAATTCGAACGAT
Clon_DF4_50 --TGAA-----CAATGAA-----------ACACTTGA----TT--CTCAATTCGAACGAT
Clon_DF19 --TGAA-----CAATGAA-----------ACACTTGA----TT--CTCAATTCGAACGAT
Clon_DF3 --TGAA-----CAATGAA-----------ACACTTGA----TT--CTCAATTCGAACGAT
Clon_DF5 --TGAA-----CAATGAA-----------ACACTTGA----TT--CTCAATTCGAACGAT
Clon_DF2 --TGAA-----CAATGAA-----------ACACTTGA----TT--CTCAATTCGAACGAT
Clon_DF7 --TGAA-----CAATGAA-----------ACACTTGA----TT--CTCAATTCGAACGAT
Clon_AS15 GCTAAAGAAACAATCA-------------ATATCCAAACCTTT--ACCA-CTTGAACGC-
Clon_AS14 GCTAAAGAAACAATCA-------------ATATCCAAACCTTT--ACCA-CTTGAACGC-
Clon_AS20 GCTAAAGAAACAATCA-------------ATATCCAAACCTTT--ACCA-CTTGAACGC-
Clon_AS13 GCTAAAGAAACAATCA-------------ATATCCAAACCTTT--ACCA-CTTGAACGC-
Clon_AS10 GCTAAAGAAACAATCA-------------ATATCCAAACCTTT--ACCA-CTTGAACGC-
Clon_AS11 GCTAAAGAAACAATCA-------------ATATCCAAACCTTT--ACCT-CTTGAACGC-
Clon_AS2 GCTAAAGAAACAATCA-------------ATATCCAAACCTTT--ACCT-CTTGAACGC-
Clon_AS12 GCTAAAGAAACAATCA-------------ATATCCAAACCTTT--ACCA-CTTGAACGC-
Clon_AS1 GCTAAAGAAACAATCA-------------ATATCCAAACCTTT--ACCA-CTTGAACGC-
Clon_AS16 GCTAAAGAAACAATCA-------------ATATCCAAACCTTT--ACCT-CTTGAACGC-
Clon_B78 ATTCA--------TCA-------------TCATGCTAAATTTT--ATC----TAAA----
Clon_BT9 ATTCA--------TCA-------------TCATGCTAAATTTT--ATC----TAAA----
Clon_BT16 ATTCA--------TCA-------------TCATGCTAAATTTT--ATC----TAAA----
Clon_BT3 ATTCA--------TCA-------------TCATGCTAAATTTT--ATC----TAAA----
Clon_BT14 ATTCA--------TCA-------------T---GCTAAATTTT--ATC----TAAA----
Clon_BT17 ATTCA--------TCA-------------TCATGCTAAATTTT--ATC----TAAA----
Clon_BT13 ATTCA--------TCA-------------T---GCTAAATTTT--ATC----TAAA----
Clon_BT1 ATTCA--------TCA-------------TCATGCTAA-TTTT--ATC----TAAA----
Clon_BT10 ATTCA--------TCA-------------TCATGCTAAATTTT--ATC----TAAA----
Clon_BT15 ATTCA--------TCA-------------TCATGCTAAATTTT--ATC----TAAA----
Clon_TPA1_20 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TPA1_22 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TPA1_29 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TPA1_28 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TPA1_26 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TPA1_21 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TPA1_36 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TPA1_27 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TPA1_23 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TPA1_1 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TF22 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TF24 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TF3 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TF2 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TF23 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TF1 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TF4 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TF7 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TF15 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_TF14 GCCAC------TGTCAC------------GTATCCAAACCTTT---TTG-CTTGAACGC-
Clon_DP8 GCTTAA-----AAGAA-------------ACATTCGA----TT--ATCAATTCGAACGAA
Clon_DP1 GCTTAA-----AAGAA-------------ACATTCGA----TT--ATCAATTCGAACGAA
Clon_DP7 GCTTAA-----AAGAA-------------ACATTCGA----TT--ATCAATTCGAACGAA
Clon_DP3 GCTTAA-----AAGAA-------------ACATTCGA----TT--ATCAATTCGAACGAA
Clon_DP6 GCTTAA-----AAGAA-------------ACATTCGA----TT--ATCAATTCGAACGAA
Clon_DP9 GCTTAA-----AAGAA-------------ACATTCGA----TT--ATCAATTCGAACGAA
Clon_DP2 GCCCAA-----AAGAA-------------ACATTCGA----TT--ATCAATTCGAACGAA
Clon_DP4 GCCCAA-----AAGAA-------------ACATTCGA----TT--ATCAATTCGAACGAA
Clon_DP10 GCCCAA-----AAGAA-------------ACATTCGA----TT--ATCAATTCGAACGAA
Clon_DP5 GCCCAA-----AAGAA-------------ACATTCGA----TT--ATCAATTCGAACGAA
Clon_EM4 GCT-GT-----CGGAA-------------ACATTCGA----TT--ATCAATTCGAACGA-
Clon_EM21 GCT-GT-----CGGAA-------------ACATTCGA----TT--ATCAATTCGAACGA-
Clon_EM2 GCT-GT-----CGGAA-------------ACATTCGA----TT--ATCAATTCGAACGA-
Clon_EM23 GCT-GT-----CGGAA-------------ACATTCGA----TT--ATCAATTCGAACGA-
Clon_EM3 GCT-GT-----CGGAA-------------ACATTCGA----TT--ATCAATTCGAACGA-
Clon_EM24 GCT-GT-----CGGAA-------------ACATTCGA----TT--ATCAATTCGAACGA-
Clon_EM22 GCT-GT-----CGGAA-------------ACATTCGA----TT--ATCAATTCGAACGA-
Clon_EM1 GCT-GA-----CGGAA-------------ACATTCGA----TT--ATCAATTCGAACGA-
Clon_EM6 GCT-GT-----CGGAA-------------ACATTCGA----TT--ATCAATTCGAACGA-
Clon_EM5 GCT-GA-----CGGAA-------------ACATTCGA----TT--ATCAATTCGAACGA-
Clon_GD1 -TTGTAA----TATTAAA-ACGAGCATCAATATCCGAACCTTTCAAAAAAATCGAACGA-
Clon_GD10 ATTGTTT----TATCATACACGAGCATCAATATCCGAACCTTTCAAAAAAATCGAACGA-
Clon_GD2 ATTGTTT----TATCATACACGAGCATCAATATCCGAACCTTTCAAAAAAATCGAACGA-
Clon_GD5 ATTGTTT----TATCATACACGAGCATCAATATCCGAACCTTTCAAAAAAATCGAACGA-
Clon_GD3 ATTGTTT----TATCATACACGAGCATCAATATCCGAACCTTTCAAAAAAATCGAACGA-
Clon_GD12 ATTGTTT----TATCATACACGAGCATCAATATCCGAACCTTTCAAAAAAATCGAACGA-
Clon_GD7 ATTGTTT----TATCATACACGAGCATCAATATCCGAACCTTTCAAAAAAATCGAACGA-
Clon_GD9 ATTGTTT----TATCATACACGAGCATCAATATCCGAACCTTTCAAAAAAATCGAACGA-
Clon_GD8 ATTGTTT----TATCATACACGAGCATCAATATCCGAACCTTTCAAAAAAATCGAACGA-
Clon_GD13 ATTGTTT----TATCATACACGAGCATCAATATCCGAACCTTTCAAAAAAATCGAACGA-
Clon_LD5 --TGCA-----TGTTGGAACAAAGCAAAAATATCCGAACCTTTCAAACAAATCGAACGA-
Clon_LD13 --TGCA-----TGTTGGAACAAAGCAAAAATATCCGAACCTTTCAAACAAATCGAACGA-
Clon_LD14 --TGCA-----TGTTGGAACAAAGCAAAAATATCCGAACCTTTCAAACAAATCGAACGA-
Clon_LD1 --TGCA-----TGTTGGAACAAAGCAAAAATATCCGAACCTTTCAAACAAATCGAACGA-
Clon_LD11 --TGCA-----TGTTGGAACAAAGCAAAAATATCCGAACCTTTCAAACAAATCGAACGA-
Clon_LD3 --TGCA-----TGTTGGAACAAAGCAAAAATATCCGAACCTTTCAAACAAATCGAACGA-
Clon_LD2 --TGCA-----TGTTGGAACAAAGCAAAAATATCCGAACCTTTCAAACAAATCGAACGA-
Clon_LD12 --TGCA-----TGTTGGAACAAAGCAAAAATATCCGAACCTTTCAAACAAATCGAACGA-
Clon_LD8 --TGCA-----TGTTGGAACAAAGCAAAAATATCCGAACCTTTCAAACAAATCGAACGA-
Clon_LD15 --TGCA-----TGTTGGAACAAAGCAAAAATATCCGAACCTTTCAAACAAATCGAACGA-
* * **
Clon_DM1 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DM21 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DM6 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DM20 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DM9 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DM12 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DM7 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DM11 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DM14 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DMA AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DF1 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DF6 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DF4 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DF26 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DF4_50 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DF19 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DF3 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DF5 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DF2 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DF7 AAGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_AS15 CTATTGCCCGTTAGCATACCCCC------ATGCTAATGAGCTGATCATGCGTTGGTT-CT
Clon_AS14 CTATTGCCCGTTAGCATACCCCC------ATGCTAATGAGCTGATCATGCGTTGGTT-CT
Clon_AS20 CTATTGCCCGTTAGCATACCCCC------ATGCTAATGAGCTGATCATGCGTTGGTT-CT
Clon_AS13 CTATTGCCCGTTAGCATACCCCC------ATGCTAATGAGCTGATCATGCGTTGGTT-CT
Clon_AS10 CTATTGCCCGTTAGCATACCCCC------ATGCTAATGAGCTGATCATGCGTTGGTTTCT
Clon_AS11 CTATTGCCCGTTAGCATATCC--------ATGCTAATGAGCTGATCATGCGTTGGTT-CT
Clon_AS2 CTATTGCCCGTTAGCATACCCCC------ATGCTAATGAGCTGATCATGCGTTGGTT-CT
Clon_AS12 CTATTGCCCGTTAGCATACCCCC------ATGCTAATGAGCTGATCATGCGTTGGTT-CT
Clon_AS1 CTATTGCCCGTTAGCATATCC--------ATGCTAATGAGCTGACCATGCGTTGGTT-CT
Clon_AS16 CTATTGCCCGTTAGCATATCC--------ATGCTAATGAGCTGATCATGCGTTGGTT-CT
Clon_B78 ---TTTTGTGTTTTCGGAC----------------ACGAAGCCAT----------TT---
Clon_B79 ---TTTTGTGTTTTCGGAC----------------ACGAAGCCAT----------TT---
Clon_B716 ---TTTTGTGTTTTCGGAC----------------ACGAAGCCAT----------TT---
Clon_B73 ---TTTTGTGTTTTCGGAC----------------ACGAAGCCAT----------TT---
Clon_B714 ---TTTTGTGTTTTCGGAC----------------ACGAA-TATT----------TT---
Clon_B717 ---TTTTGTGTTTTCGGAC----------------ACGAA-TATT----------TT---
Clon_B713 ---TTTTGTGTTTTCGGAC----------------ACGAAGCCAT----------TT---
Clon_B71 ---TTTTGTGTTTTCGGAC----------------ACGAAGCCAT----------TT---
Clon_B710 ---TTTTGTGTTTTCGGAC----------------ACGAA-TTAT----------TT---
Clon_B715 ---TTTTGTGTTTTCGGAC----------------ACGAA-TTAT----------TT---
Clon_TPA1_20 AAATTGCCCGTTACCA-GAAGTA-----ACCAAAA-TGGGCTTATCATG------TT---
Clon_TPA1_22 AAATTGCCCGTTACCA-GAAGTA-----ACCAAAA-TGGGCTTATCATG------TT---
Clon_TPA1_29 AAATTGCCCGTTACCA-GAAGTA-----ACCAAAA-TGGGCTTATCATG------TT---
Clon_TPA1_28 AAATTGCCCGTTACCA-GAAGTA-----ACCAAAA-TGGGCTTATCATG------TT---
Clon_TPA1_26 AAATTGCCCGTTACCA-AATGTA-----ACCAAAA-TGGGCTTATCATG------TT---
Clon_TPA1_21 AAATTGCCCGTTACCA-AATGTA-----ACCAAAA-TGGGCTTATCATG------TT---
Clon_TPA1_36 AAATTGCCCGTTACCA-AATGTA-----ACCAAAA-TGGGCTTATCATG------TT---
Clon_TPA1_27 AAATTGCCCGTTACCA-AATGTA-----ATCAAAA-TGGGCTTATCATG------TT---
Clon_TPA1_23 AAATTGCCCGTTACCA-AATGTA-----ACCAAAAATGGGCTTATCATG------TT---
Clon_TPA1_1 AAATTGCCCGTTACCA-AATGTA-----ACCAAAAATGGGCTTATCATG------TT---
Clon_TF22 AAATTGCCCGTTACCAAAA---------GCTAAAAATGGGCTTATCATG------TT---
Clon_TF24 AAATTGCCCGTTACCAAAA---------GCTAAAAATGGGCTTATCATG------TT---
Clon_TF3 AAATTGCCCGTTACCAAAA---------GCTAAAAATGGGCTTATCATG------TT---
Clon_TF2 AAATTGCCCGTTACCAAAA---------GCTAAAAATGGGCTTATCATG------TT---
Clon_TF23 AAATTGCCCGTTACCAAAA---------GCTAAAAATGGGCTTATCATG------TT---
Clon_TF1 AAATTGCCCGTTACCAAAA---------GCTAAAAATGGGCTTATCATG------TT---
Clon_TF4 AAATTGCCCGTTACCAAAA---------GCTAAAAATGGGCTTATCATG------TT---
Clon_TF7 AAATTGCCCGTTACCAAAA---------GCTAAAAATGGGCTTATCATG------TT---
Clon_TF15 AAATTGCCCGTTACCAAAA---------GCTAAAAATGGGCTTATCATG------TT---
Clon_TF14 AAATTGCCCGTTACCAAAA---------GCTAAAAATGGGCTTATCATG------TT---
Clon_DP8 TCGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DP1 TCGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DP7 TCGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DP3 AAGTTGCCCGTTATCACAA----------------ATGGATCGAC-----------T---
Clon_DP6 AAGTTGCCCGTTATCACAA----------------ATGGATCGAC-----------T---
Clon_DP9 AAGTTGCCCGTTATCACAA----------------ATGGATCGAC-----------T---
Clon_DP2 TCGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DP4 TCGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DP10 TCGTTGCCCGTTATCACAA----------------ACGGATCGAC-----------T---
Clon_DP5 AAGTTGCCCGTTATCACAA----------------ATGGATCGAC-----------T---
Clon_EM4 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------T---
Clon_EM21 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------T---
Clon_EM2 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------T---
Clon_EM23 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------T---
Clon_EM3 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------T---
Clon_EM24 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------T---
Clon_EM22 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------T---
Clon_EM1 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------T---
Clon_EM6 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------T---
Clon_EM5 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------T---
Clon_GD1 CAGTTGCCCGTTATCACAA----------------ATGGAACGAC-----------TGGC
Clon_GD10 TAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGGC
Clon_GD2 TAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGGC
Clon_GD5 TAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGGC
Clon_GD3 TAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGGC
Clon_GD12 TAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGGC
Clon_GD7 TAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGGC
Clon_GD9 TAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGGC
Clon_GD8 CAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGGC
Clon_GD13 CAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGGC
Clon_LD5 AAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGAT
Clon_LD13 AAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGAT
Clon_LD14 AAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGAT
Clon_LD1 AAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGAT
Clon_LD11 AAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGAT
Clon_LD3 AAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGAT
Clon_LD2 AAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGAT
Clon_LD12 AAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGAT
Clon_LD8 AAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGAT
Clon_LD15 AAGTTGCCCGTTATCAAAA----------------ATGGGTTGAC-----------TGAT
** * * * * *
Clon_DM1 -GACTTG----TGTGTTGCG-----------------------------------ATCGG
Clon_DM21 -GACTTG----TGTGTTGCG-----------------------------------ATCGG
Clon_DM6 -GACTTG----TGTGTTGCG-----------------------------------ATCGG
Clon_DM20 -GACTTG----TGTGTTGCG-----------------------------------ATCGG
Clon_DM9 -GACTTG----TGTGTTGCG-----------------------------------ATCGG
Clon_DM12 -GACTTG----TGTGTTGCG-----------------------------------ATCGG
Clon_DM7 -GACTT-----TGTGTTGCG-----------------------------------ATCGG
Clon_DM11 -GACTTG----TGTGTTGCG-----------------------------------ATCGG
Clon_DM14 -GACTTG----TGTGTTGCG-----------------------------------ATCGG
Clon_DMA -GACTTG----TGTGTTGCG-----------------------------------ATCGG
Clon_DF1 -GACTT------GTGTTGCG-----------------------------------ATCGG
Clon_DF6 -GACTT------GTGTTGCG-----------------------------------ATTGG
Clon_DF4 -GACTT------GTGTTGCG-----------------------------------ATCGG
Clon_DF26 -GACTT------GTGTTGCG-----------------------------------ATCGG
Clon_DF4_50 -GACTT------GTGTTGCG-----------------------------------ATTGG
Clon_DF19 -GACTT------GTGTTGCG-----------------------------------ATTGG
Clon_DF3 -GACTT------GTGTTGCG-----------------------------------ATTGG
Clon_DF5 -GACTT------GTGTTGCG-----------------------------------ATCGG
Clon_DF2 -GACTT------GTGTTGCG-----------------------------------ATCGG
Clon_DF7 -GACTT------GTGTTGCG-----------------------------------ATCGG
Clon_AS15 ACATGTC----AGTGTGACCCAGAGAAAGGCTACCAACCCTT-----------TACTCGG
Clon_AS14 ACATGTC----AGTGTGACCCAGAGAAAGGCTACCAACCCTT-----------TACTCGG
Clon_AS20 ACATGTC----AGTGTGACCCAGAGAAAGGCTACCAACCCTT-----------TACTCGG
Clon_AS13 ACATGTC----AGTGTGACCCAGAGAAAGGCTACCAACCCTT-----------TACTCGG
Clon_AS10 ACATGTC----AGTGTGACCCAGAGAAAGGCTACCATCCCTT-----------TACTCGG
Clon_AS11 ACATGTC----AGTGTGACCCAGAGAAAGGCTACCA---CCT-----------TACTCGG
Clon_AS2 ACATGTC----AGTGTGACCCAGAGAAAGGCTACCA---CCT-----------TACTCGG
Clon_AS12 ACATGTC----AGTGTGACCCAGAGAAAGGCTACCAACCCCT-----------TACTCGG
Clon_AS1 ACATGTC----AGTGTGACCCAGAGAAAGGCTACCA-CCCCT-----------TACTCGG
Clon_AS16 ACATGTC----AGTGTGACCCAGAGAAAGGCTACCAACCCCT-----------TACTCGG
Clon_BT8 ---CGTT----AGT-TGATC----------------------------------ATTGAG
Clon_BT9 ---CGTT----AGT-TGATC----------------------------------ATTGAG
Clon_BT16 ---CGTT----TGT-TGATC----------------------------------ATTGAG
Clon_BT3 ---CGTT----AGT-TGATC----------------------------------ATTGAG
Clon_BT14 ---TGCT----AGT-TGATC----------------------------------ATTGAG
Clon_BT17 ---TGCT----AGT-TGATC----------------------------------ATTGAG
Clon_BT13 ---CGTT----AGT-TGATC----------------------------------ATTGAG
Clon_BT1 ---CGTT----AGT-TGATC----------------------------------ATTGAG
Clon_BT10 ---TGCT----AGT-TGATC----------------------------------ATTGAG
Clon_BT15 ---TGCT----AGT-TGATC----------------------------------ATTGGG
Clon_TPA1_20 AGGC--G----AGT-TGTGTCC-------------------------------GTGTGTG
Clon_TPA1_22 AGGC--G----AGT-TGTGTCC-------------------------------GTGTGTG
Clon_TPA1_29 AGGC--G----AGT-TGTGTCC-------------------------------GTGTGTG
Clon_TPA1_28 AGGC--G----AGT-TGTGTCC-------------------------------GTGTGTG
Clon_TPA1_26 AGGCC-G----AGT-TGTGTCC-------------------------------GTGTGTG
Clon_TPA1_21 AGGCC-G----AGT-TGTGTCC-------------------------------GTGTGTG
Clon_TPA1_36 AGGCC-G----AGT-TGTGTCC-------------------------------GTGTGTG
Clon_TPA1_27 AGGC--G----AGA-TGTGTCC-------------------------------GTGTGTG
Clon_TPA1_23 AGGC--G----AGT-TGTGTCC-------------------------------GTGTGTG
Clon_TPA1_1 AGGC--G----AGT-TATGTCC-------------------------------GTGTGTG
Clon_TF22 AGGCGAG----AGT---TGT-----------------------------------GCGTG
Clon_TF24 AGGCGAG----AGT---TGT-----------------------------------GCGTG
Clon_TF3 AGGCGAG----AGT---TGT-----------------------------------GCGTG
Clon_TF2 AGGCGAG----AGT---TGT-----------------------------------GCGTG
Clon_TF23 AGGCGAG----AGT---TGT-----------------------------------GCGTG
Clon_TF1 AGGCGAG----AGT---TGT-----------------------------------GCGTG
Clon_TF4 AGGCGAG----AGT---TGT-----------------------------------GCGTG
Clon_TF7 AGGCGAG----AGT---TGT-----------------------------------GCGTG
Clon_TF15 AGGCGAG----AGT---TGT-----------------------------------GCGTG
Clon_TF14 AGGCGAG----AGT---TGT-----------------------------------GCGTG
Clon_DP8 -GGCAAT----TGT----------------------------------------TGACAG
Clon_DP1 -GGCAAT----TGT----------------------------------------TGACAG
Clon_DP7 -GGCAAT----TGT----------------------------------------TGACAT
Clon_DP3 -GGCAAT----TGT----------------------------------------TGACAC
Clon_DP6 -GGCAAT----TGT----------------------------------------TGACAC
Clon_DP9 -GGCAAT----TGT----------------------------------------TGACAC
Clon_DP2 -GGCAAT----TGT----------------------------------------TGACAT
Clon_DP4 -GGCAAT----TGT----------------------------------------TGACAT
Clon_DP10 -GGCAAT----TGT----------------------------------------TGACAC
Clon_DP5 -GGCAAT----TGT----------------------------------------TGACAT
Clon_EM4 -GGCAAT----TGT----------------------------------------TGTGAT
Clon_EM21 -GGCAAT----TGT----------------------------------------TGTGAT
Clon_EM2 -GGCAAT----TGT----------------------------------------TGTGAT
Clon_EM23 -GGCAAT----TGT----------------------------------------TGTGAT
Clon_EM3 -GGCAAT----TGT----------------------------------------TGTGAT
Clon_EM24 -GGCAAT----TGT----------------------------------------TGTGAT
Clon_EM22 -GGCAAT----TGT----------------------------------------TGTGAT
Clon_EM1 -GGCAAT----TGT----------------------------------------TGTGAT
Clon_EM6 -GGCAAT----TGT----------------------------------------TGTGAT
Clon_EM5 -GGCAAT----TGT----------------------------------------TGTGAT
Clon_GD1 GAGCGAT-TATGAT---------------TGT-A--------------------ATCTAA
Clon_GD10 GAGCGAT-TATGAT---------------TGT-A--------------------ATCTAA
Clon_GD2 GAGCGAT-C--GAT---------------TATGA--------------------ATCGAG
Clon_GD5 GAGCGAT-C--GAT---------------TATGA--------------------ATCGAG
Clon_GD3 GAGCGAT-C--GAT---------------TATGA--------------------ATCGAG
Clon_GD12 GAGCGAT-C--GAT---------------TATGA--------------------ATCGAG
Clon_GD7 GAGCGAT-C--GAT---------------TATGA--------------------ATCGAG
Clon_GD9 GAGCGAT-C--GAT---------------TATGA--------------------ATCGAG
Clon_GD8 GAGCGAT-C--GAT---------------TATGA--------------------ATCGAG
Clon_GD13 GAGCGAT-C--GAT---------------TATGA--------------------ATCGAG
Clon_LD5 GAACGATGTGCGATGTAACCGCGTGCATGCATGGT-------------------ATCGAG
Clon_LD13 GAACGATGTGCGATGTAACCGCGTGCATGCATGGT-------------------ATCGAG
Clon_LD14 GAACGATGTGCGATGTAACCGCGTGCATGCATGGT-------------------ATCGAG
Clon_LD1 GAACGATGTGCGATGTAACCGCGTGCATGCATGGT-------------------ATCGAG
Clon_LD11 GAACGATGTGCGATGTAACCGCGTGCATGCATGGT-------------------ATCGAG
Clon_LD3 GAACGATGTGCGATGTAACCGCGTGCATGCATGGT-------------------ATCGAG
Clon_LD2 GAACGATGTGCGATGTAACCGCGTGCATGCATGGT-------------------ATCGAG
Clon_LD12 GAACGATGTGCGATGTAACCGCATGCATGCATGGT-------------------ATCGAG
Clon_LD8 GAACGATGTGCGATGTAACCGCGTGCATGCATGGT-------------------ATCGAG
Clon_LD15 GAACGATGTGCGATGTAACCGCGTGCATGCATGGT-------------------ATCGAG
Clon_DM1 TGGTGT-CA-------------CATTGAAAGTTCTGAAGT-------TAA----------
Clon_DM21 TGGTGT-CA---------------TTGAAAGTTCTGAAGTG-----TTAA----------
Clon_DM6 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TAA----------
Clon_DM20 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TAA----------
Clon_DM9 TGGTGT-CA-------------CATTGAAAGTTCTGAAGT-------TAA----------
Clon_DM12 TGGTGT-CA-------------CATTGAAAGTTCTGAAGT-------TAA----------
Clon_DM7 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAT---------
Clon_DM11 TGGTGT-CA-------------CATTGAAAGTTCTGAAGT-------TAA----------
Clon_DM14 TGGTGT-CA---------------TTGAAAGTTCTGAAGTG-----TTAA----------
Clon_DMA TGGTGT-CA---------------TTGAAAGTTCTGAAGTA-----TTAA----------
Clon_DF1 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAA---------
Clon_DF6 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAA---------
Clon_DF4 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAA---------
Clon_DF26 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAA---------
Clon_DF4_50 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAA---------
Clon_DF19 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAA---------
Clon_DF3 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAA---------
Clon_DF5 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAA---------
Clon_DF2 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAA---------
Clon_DF7 TGGTGT-CA---------------TTGAAAGTTCTGAAGT-------TGAA---------
Clon_AS15 TACAAC----------------TTTTGAACGCTTGCCTGT------TTAACT-TGTCTGT
Clon_AS14 TACAAC----------------TTTTGAACGCTTGCCTGT------TTAACT-TGTCTGT
Clon_AS20 TACAAC----------------TTTTGAACGCTTGCCTGT------TTAACT-TGTCTGT
Clon_AS13 TACAAC----------------TTTTGAACGCTTGCCTGT------TTAACT-TGTCTGT
Clon_AS10 TACAAC----------------TTTTGAACGCTTGCCTGT------TTAACT-TGTCTGT
Clon_AS11 TACAACATC-------------CTTTGAACGCTTGCCTGT------TTAACT-TGTCTGT
Clon_AS2 TACAACATC-------------CTTTGAACGCTTGCCTGT------TTAACT-TGTCTGT
Clon_AS12 TACAACATC-------------CTTTGAACGCTTGCCTGT------TTAACT-TGTCTGT
Clon_AS1 TACAACATC-------------CTTTGAACGCTTGCCTGT------TTAACT-TGTCTGT
Clon_AS16 TACAACATC-------------CTTTGAACGCTTGCCTGT------TTAACT-TGTCTGT
Clon_BT8 TAGCAT----------------TCCTG--TGATTGTC-GT------TTGA----------
Clon_BT9 TAGCAT----------------TCCTG--TGATTGTC-GT------TTGA----------
Clon_BT16 TAGCAT----------------TCCTG--TGATTGTC-TT------TTGA----------
Clon_BT3 TAGCAT----------------TCCTG--TGATTGTC-GT------TTGA----------
Clon_BT14 TAGCAT----------------TCCTG--TGATTGTC-GT------TTGA----------
Clon_BT17 TAGCAT----------------TCCTG--TGATTGTC-GT------TTGA----------
Clon_BT13 TAGCAT----------------TCCTG--TGATTGTC-TT------TTGA----------
Clon_BT1 TAGCAT----------------TCCTG--TGATTGTC-AT------TTGA----------
Clon_BT10 TAGCAT----------------TCCTG--TGATTGTC-TT------TTGA----------
Clon_BT15 TAGCAT----------------TCCTG--TGATTGTC-TT------TTGA----------
Clon_TPA1_20 TGGTACACC-------------CGTTGAACGCTTGAG-AC-------TAA----GC--AG
Clon_TPA1_22 TGGTACACC-------------CGTTGAACGCTTGAG-AC-------TAA----GC--AG
Clon_TPA1_29 TGGTACACC-------------CGTTGAACGCTTGAG-AC-------TAA----GC--AG
Clon_TPA1_28 TGGTACACC-------------CGTTGAACGCTTGAG-AC-------TAA----GC--AG
Clon_TPA1_26 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TPA1_21 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TPA1_36 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TPA1_27 TGGTACACC-------------CGTTGAACGCTTGAG-AC-------TAA----GC--AG
Clon_TPA1_23 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TPA1_1 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TF22 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TF24 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TF3 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TF2 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TF23 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TF1 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TF4 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TF7 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TF15 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_TF14 TGGTACACC-------------CGTTGAACGCTTGAG-GC-------TAA----GC--AG
Clon_DP8 TGATGAGGT-------------TATTGAAAGCTC-------------TGA----------
Clon_DP1 TGATGAGGT-------------TATTGAAAGCTC-------------TGA----------
Clon_DP7 TGATGAGGT-------------TATTGAAAGCTC-------------TGA----------
Clon_DP3 TGATGAGGT-------------TATTGAAAGCTC-------------TGA----------
Clon_DP6 TGATGAGGT-------------TATTGAAAGCTC-------------TGA----------
Clon_DP9 TGATGAGGT-------------TATTGAAAGCTC-------------TGA----------
Clon_DP2 TGATGAGGT-------------TATTGAAAGCTC-------------TGA----------
Clon_DP4 TGATGAGGT-------------TATTGAAAGCTC-------------TGA----------
Clon_DP10 TGATGAGGT-------------TATTGAAAGCTC-------------TGA----------
Clon_DP5 TGATGAGGT-------------TATTGAAAGCTC-------------TGA----------
Clon_EM4 TGAGGTTGT-------------CATTGAAAGCTC-------------TGA----------
Clon_EM21 TGAGGTTGT-------------CATTGAAAGCTC-------------TGA----------
Clon_EM2 TGAGGTTGT-------------CATTGAAAGCTC-------------TGA----------
Clon_EM23 TGAGGTTGT-------------CATTGAAAGCTC-------------TGA----------
Clon_EM3 TGAGGTTGT-------------CATTGAAAGCTC-------------TGA----------
Clon_EM24 TGAGGTTGT-------------CATTGAAAGCTC-------------TGA----------
Clon_EM22 TGAGGTTGT-------------CATTGAAAGCTC-------------TGA----------
Clon_EM1 TGAGGTTGT-------------CATTGAAAGCTC-------------TGA----------
Clon_EM6 TGAGGTTGT-------------CATTGAAAGCTC-------------TGA----------
Clon_EM5 TGAGGTTGT-------------CATTGAAAGCTC-------------TGA----------
Clon_GD1 GGTGTAAAA-----------TGCATCGAAAGCTTAATGTT------GCAATTGTGCATAA
Clon_GD10 GGTGTAAAA-----------TGCATCGAAAGCTTAATGTT------GCAATTGTGCATAA
Clon_GD2 TGTTGTGGA-----------TGCATCGAAAGCTTAATGTT------GCAATTGTGCATAC
Clon_GD5 TGTTGTGGA-----------TGCATCGAAAGCTTAATGTT------GCAATTGTGCATAC
Clon_GD3 TGTTGTGGA-----------TGCATCGAAAGCTTAATGTT------GCAATTGTGCATAC
Clon_GD12 TGTTGTGGA-----------TGCATCGAAAGCTTAATGTT------GCAATTGTGCATAC
Clon_GD7 TGTTGTGGA-----------TGCATCGAAAGCTTAATGTT------GCAATTGTGCATAC
Clon_GD9 TGTTGTGGA-----------TGCATCGAAAGCTTAATGTT------GCAATTGTGCATAC
Clon_GD8 TGTTGTGGA-----------TGCATCGAAAGCTTAATGTT------GCAATTGTGCATAC
Clon_GD13 TGTTGTGGA-----------TGCATCGAAAGCTTAATGTT------GCAATTGTGCATAC
Clon_LD5 AAATGTGAAAAGATGTGCAATGTATCGAAAGCTTGATGTT------TTGCTTGCTTG-AT
Clon_LD13 AAATGTGAAAAGATGTGCAATGTATCGAAAGCTTGATGTT------TTGCTTGCTTG-AT
Clon_LD14 AAATGTGAAAAGATGTGCAATGTATCGAAAGCTTGATGTT------TTGCTTGCTTG-AT
Clon_LD1 AAATGTGAAAAGATGTGCAATGTATCGAAAGCTTGATGTT------TTGCTTGCTTG-AT
Clon_LD11 AAATGTGAAAAGATGTGCAATGTATCGAAAGCTTGATGTT------TTGCTTGCTTG-AT
Clon_LD3 AAATGTGAAAAGATGTGCAATGTATCGAAAGCTTGATGTT------TTGCTTGCTTG-AT
Clon_LD2 AAATGTGAAAAGATGTGCAATGTATCGAAAGCTTGATGTT------TTGCTTGCTTG-AT
Clon_LD12 AAATGTGAAAAGATGTGCAATGTATCGAAAGCTTGATGTT------TTGCTTGCTTG-AT
Clon_LD8 AAATGTGAAAAGATGTGCAATGTATCGAAAGCTTGATGTT------TTGCTTGCTTG-AT
Clon_LD15 AAATGTGAAAAGATGTGCAATGTATCGAAAGCTTGATGTT------TTGCTTGCTTG-AT
* * *
Clon_DM1 ----ACTTG-------------------GCTT-------------TATA----------T
Clon_DM21 ----ACTTG-------------------GCTT-------------TATA----------T
Clon_DM6 ----ACTTG-------------------GCTT-------------TATA----------T
Clon_DM20 ----ACTTG-------------------GCTT-------------TATA----------T
Clon_DM9 ----ACTTG-------------------GCTT-------------TATA----------T
Clon_DM12 ----ACTTG-------------------GCTT-------------TATA----------T
Clon_DM7 ----ACTTG-------------------GCTT-------------TATA----------T
Clon_DM11 ----ACTTG-------------------GCTT-------------TATA----------T
Clon_DM14 ----ACTTG-------------------GCTT-------------TATA----------T
Clon_DMA ----ACTTG-------------------GCTT-------------TATA----------T
Clon_DF1 ----ACTTG-------------------GCTT-------------TT------------T
Clon_DF6 ----ACTTG-------------------GCTT-------------TA------------T
Clon_DF4 ----ACTTG-------------------GCTT-------------TA------------T
Clon_DF26 ----ACTTG-------------------GCTT-------------TA------------T
Clon_DF4_50 ----ACTTG-------------------GCTT-------------TA------------T
Clon_DF19 ----ACTTG-------------------GCTT-------------TA------------T
Clon_DF3 ----ACTTG-------------------GCTT-------------TA------------T
Clon_DF5 ----ACTTG-------------------GCTT-------------TA------------T
Clon_DF2 ----ACTTG-------------------GCTT-------------TA------------T
Clon_DF7 ----ACTTG-------------------GCTT-------------TA------------T
Clon_AS15 GCACGCTT---TGCATACTGTCTCTACCACGCCAAATAAACCT-TTTAGG---------T
Clon_AS14 GCACGCTT---TGCATACTGTCTCTACCACGCCAAATAAACCT-TTTAGG---------T
Clon_AS20 GCACGCTT---TGCATACTGTCTCTACCACGCCAAATAAACCT-TTTAGG---------T
Clon_AS13 GCACGCTT---TGCATACTGTCTCTACCACGCCAAATAAACCT-TTTAGG---------T
Clon_AS10 GCACGCTT---TGCATACTGTCTCTACCACGCCAAATAAACCT-TTTAGG---------T
Clon_AS11 GCACGCTT---TGCATACTGTCTCTACCACGCCAAATAAACCT-TTTAGG---------T
Clon_AS2 GCACGCTT---TGCATACTGTCTCTACCACGCCAAATAAACCT-TTTAGG---------T
Clon_AS12 GCACGCTT---TGCATACTGTCTCTACCACGCCAAATAAACCT-TTTAGG---------T
Clon_AS1 GCACGCTT---TGCATACTGTCTCTACCACGCCAAATAAACCCGTTTAGG---------T
Clon_AS16 GCACGCTT---TGCATACTGTCTCTACCACGCCAAATAAACCT-TTTAGG---------T
Clon_BT8 --ACGCTT---TGCA-------------ACATTA----------TTTTGA---------T
Clon_BT9 --ACGCTT---TGCA-------------ACATTA----------TTTTGA---------T
Clon_BT16 --ACGCTT---TGCA-------------ACATTA----------TTTTGA---------T
Clon_BT3 --ACGCTT---TGCA-------------ACATTA----------TTTTGA---------T
Clon_BT14 --ACGCTT---TGCA-------------ACATTA----------TTTTGA---------T
Clon_BT17 --ACGCTT---TGCA-------------ACATTA----------TTTTGA---------T
Clon_BT13 --ACGCTT---TGCA-------------ACATTA----------TTTTGA---------T
Clon_BT1 --ACGCTT---TGCA-------------ACATTA----------TTTTGA---------T
Clon_BT10 --ACGTTA---TGCA-------------ACATT-----------TATTGG---------T
Clon_BT15 --ACGCTA---TGCA-------------ACATT-----------TATTGG---------T
Clon_TPA1_20 A---GTT----TGCAA-GAGGCTG----GCGCCAG-----------CAAC---------A
Clon_TPA1_22 A---GTT----TGCAA-GAGGCTG----GCGCCAG-----------CAAC---------A
Clon_TPA1_29 A---GTT----TGCAA-GAGGCTG----GCGCCAG-----------CAGC---------A
Clon_TPA1_28 A---GTT----TGCAA-GAGGCTG----GCGCCAG-----------CAAC---------A
Clon_TPA1_26 A---GTT----TGCAA-GAGGCTG----GCGCCAG-----------CAGC---------A
Clon_TPA1_21 A---GTT----TGCAA-GAGGCTG----GCGC--------------CAGC---------A
Clon_TPA1_36 A---GTT----TGCAA-GAGGCTG----GCGCTAG-----------CAGC---------A
Clon_TPA1_27 A---GTT----TGCAA-GAGGCTG----GCGCCAG-----------CAGC---------A
Clon_TPA1_23 A---GTT----TGCAA-GAGGCTG----GCGC--------------CAGC---------A
Clon_TPA1_1 A---GTT----TGCAA-GAGGCTG----GCGC--------------CAGC---------A
Clon_TF22 A---GTT----TACAA-GAGGCTG----GCGCCAA-----------TAGC---------A
Clon_TF24 A---GTT----TACAA-GAGGCTG----GCGCCAA-----------TAGC---------A
Clon_TF3 A---GTT----TACAA-GAGGCTG----GCGCCAA-----------TAGC---------A
Clon_TF2 A---GTT----TGCAA-GAGGCTG----GCGCCAA-----------TAGC---------A
Clon_TF23 A---GTT----TGCAA-GAGGCTG----GCGCCAA-----------TAGC---------A
Clon_TF1 A---GTT----TGCAA-GAGGCTG----GCGCCAA-----------TAGC---------A
Clon_TF4 A---GTT----TGCAA-GAGGCTG----GCGCCAA-----------TAGC---------A
Clon_TF7 A---GTT----TGCAA-GAGGCTG----GCGCCAA-----------TAGC---------A
Clon_TF15 A---GTT----TGCAA-GAGGCTG----GCGCCAA-----------TAGC---------A
Clon_TF14 A---GTT----TGCAA-GAGGCTG----GCGCCAA-----------TAGC---------A
Clon_DP8 ---AATT---------------------GTATC-------------CTGG---------T
Clon_DP1 ---AATT---------------------GTATC-------------CTGG---------T
Clon_DP7 ---AATT---------------------GTATC-------------CTGG---------T
Clon_DP3 ---AATT---------------------GTATC-------------CTGG---------T
Clon_DP6 ---AATT---------------------GTATC-------------CTGG---------T
Clon_DP9 ---AATT---------------------GTATC-------------CTGG---------T
Clon_DP2 ---AATT---------------------GTATC-------------CTGG---------T
Clon_DP4 ---AATT---------------------GTATC-------------CTGG---------T
Clon_DP10 ---AATT---------------------GTATC-------------CTGG---------T
Clon_DP5 ---AATT---------------------GTATC-------------CTGG---------T
Clon_EM4 ---AGTT---------------------TTGTC-------------CTGG---------T
Clon_EM21 ---AGTT---------------------TTGTC-------------CTGG---------T
Clon_EM2 ---AGTT---------------------GTATC-------------CTGG---------T
Clon_EM23 ---AGTT---------------------GTATC-------------CCGG---------T
Clon_EM3 ---AGTT---------------------GTATC-------------CTGG---------T
Clon_EM24 ---AGTT---------------------GTATC-------------CTGG---------T
Clon_EM22 ---AGTT---------------------GTATC-------------CTGG---------T
Clon_EM1 ---AGTT---------------------TTGTC-------------CTGG---------T
Clon_EM6 ---AGTT---------------------GTATC-------------CTGG---------T
Clon_EM5 ---AGTT---------------------GTATC-------------CTGG---------T
Clon_GD1 TG-TGTTGAAG--CAGGTTAAATG---AACGTTTAT---------ATGA----------T
Clon_GD10 TG-TGTTGAAG--CAGGTTAAATG---AACGTTTAT---------ATGA----------T
Clon_GD2 CG-TGTTGAAG--CAGGTTAAATG---AACGTTTAT---------ATGA----------T
Clon_GD5 CG-TGTTGAAG--CAGGTTAAATG---AACGTTTAT---------ATGA----------T
Clon_GD3 CG-TGTTGAAG--CAGGTTAAATG---AACGTTTAT---------ATGA----------T
Clon_GD12 CG-TGTTGAAG--CAGGTTAAATG---AACGTTTAT---------ATGA----------T
Clon_GD7 CG-TGTTGAAG--CAGGTTAAATG---AACGTTTAT---------ATGA----------T
Clon_GD9 CG-TGTTGAAG--CAGGTTAAATG---AACGTTTAT---------ATGA----------T
Clon_GD8 CG-TGTTGAAG--CAGGTTAAATG---AACGTTTAT---------ATGA----------T
Clon_GD13 CG-TGTTGAAG--CAGGTTAAATG---AACGTTTAT---------ATGA----------T
Clon_LD5 TGCTGATGATGTTCGAATCAATTGC-TAGTGTTTGTGCAGA----ATAAACAAGACATTT
Clon_LD13 TGCTGATGATGTTCGAATCAATTGC-TAGTGTTTGTGCAGA----ATAAACAAGACATTT
Clon_LD14 TGCTGATGATGTTCGAATCAATTGC-TAGTGTTTGTGCAGA----ATAAACAAGACATTT
Clon_LD1 TGCTGATGATGTTCGAATCAATTGC-TAGTGTTTGTGCAGA----ATAAACAAGACATTT
Clon_LD11 TGCTGATGATGTTCGAATCAATTGC-TAGTGTTTGTGCAGA----ATAAACAAGACATTT
Clon_LD3 TGCTGATGATGTTCGAATCAATTGC-TAGTGTTTGTGCAGA----ATAAACAAGACATTT
Clon_LD2 TGCTGATGATGTTCGAATCAATTGC-TAGTGTTTGTGCAGA----ATAAACAAGACATTT
Clon_LD12 TGCTGATGATGTTCGAATCAATTGC-TAGTGTTTGTGCAGA----ATAAACAAGACATTT
Clon_LD8 TGCTGATGATGTTCGAATCAATTGC-TAGTGTTTGTGCAGA----ATAAACAAGACATTT
Clon_LD15 TGCTGATGATGTTCGAATCAATTGC-TAGTGTTTGTGCAGA----ATAAACAAGACATTT
Clon_DM1 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DM21 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DM6 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DM20 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DM9 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DM12 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DM7 T-----A---TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DM11 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DM14 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DMA T-----A---TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DF1 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DF6 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DF4 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DF26 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DF4_50 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DF19 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DF3 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DF5 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DF2 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_DF7 T-----ATA-TCTGGGTAA-CAT------GGAAAAAAGCA--------------------
Clon_AS15 TGATTCATT-T-TGGGCAATCATAT---GAAATG-AATCATTTCCTAAAA--------AG
Clon_AS14 TGATTCATT-T-TGGGCAATCATAT---GAAATG-AATCATTTCCTAAAA--------AG
Clon_AS20 TGATTCATT-T-TGGGCAATCATAT---GAAATG-AATCATTTCCTAAAA--------AG
Clon_AS13 TGATTCATT-T-TGGGCAATCATAT---GAAATG-AATCATTTCCTAAAA--------AG
Clon_AS10 TGATTCATT-T-TGGGCAATCATAT---GAAATG-AATCATTTCCTAAAA--------AG
Clon_AS11 TGATTCATT-T-TGGGCAATCATAT---GAAATG-AATCATTTCCTAAAA--------AG
Clon_AS2 TGATTCATT-T-TGGGCAATCATAT---GAAATG-AATCATTTCCTAAAA--------AG
Clon_AS12 TGATTCATT-T-TGGGCAATCATAT---GAAATG-AATCATTTCCTAAAA--------AG
Clon_AS1 TGATTCATT-T-TGGGCAATCATAT---GAAATG-AATCATTTCCTAAAA--------AG
Clon_AS16 TGATTCATT-T-TGGGCAATCATAT---GAAATG-AATCATTTCCTAAAA--------AG
Clon_BT8 T--TT-GTG-TGTGGGGGATTT----------TGCACACA--------------------
Clon_BT9 T--TT-GTG-TGTGGGGGATTT----------TGCACACA--------------------
Clon_BT16 T--TT-GTG-TGTGGGGGATTT----------TGCACACA--------------------
Clon_BT3 T--TT-GTG-TGTGGGGGATTT----------TGCACACA--------------------
Clon_BT14 T--TT-GTG-TGTGGGGGATTT----------TGCACACA--------------------
Clon_BT17 T--TT-GTG-TGTGGGGGATTT----------TGCACACA--------------------
Clon_BT13 T--TT-GTG-TGTGGGGGATTT----------TGCACACA--------------------
Clon_BT1 T--TT-GTG-TGTGGGGGATTT----------TGCACACA--------------------
Clon_BT10 T--TT-GTG-TGTGGGGGATTT----------TGCACACAA-------------------
Clon_BT15 T--TT-GTG-TGTGGGGGATTT----------TGCACACA--------------------
Clon_TPA1_20 GCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCG--------------------
Clon_TPA1_22 GCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCG--------------------
Clon_TPA1_29 GCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCG--------------------
Clon_TPA1_28 GCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCG--------------------
Clon_TPA1_26 GCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCG--------------------
Clon_TPA1_21 GCAC-CATT-CATGGGCAATCAA-T---GGAATGTGTGCT--------------------
Clon_TPA1_36 GCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCG--------------------
Clon_TPA1_27 GCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCG--------------------
Clon_TPA1_23 GCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCG--------------------
Clon_TPA1_1 GCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCG--------------------
Clon_TF22 TCAC-CATT-TGTGGGCAATCAT-T---GGAATGTGTGCA--------------------
Clon_TF24 TCAC-CATT-TGTGGGCAATCAT-T---GGAATGTGTGCA--------------------
Clon_TF3 TCAC-CATT-TGTGGGCAATCAT-T---GGAATGTGTGCA--------------------
Clon_TF2 TCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCA--------------------
Clon_TF23 TCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCA--------------------
Clon_TF1 TCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCA--------------------
Clon_TF4 TCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCA--------------------
Clon_TF7 TCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCG--------------------
Clon_TF15 TCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCA--------------------
Clon_TF14 TCAC-CATT-CATGGGCAATCAT-T---GGAATGTGTGCA--------------------
Clon_DP8 T--TTCGTG-T-TGGGCGA-CAT------GGATGAAAACA--------------------
Clon_DP1 T--TTCGTG-T-TGGGCGA-CAT------GGATGAAAACA--------------------
Clon_DP7 T--TTCGTG-T-TGGGCGG-CAT------GGATGAAAACA--------------------
Clon_DP3 T--TTCGTG-T-TGGGCAA-CAT------GGATGAAAACA--------------------
Clon_DP6 T--TTCGTG-T-TGGGCAA-CAT------GGATGAAAACA--------------------
Clon_DP9 T--TTCGTG-T-TGGGCAA-CAT------GGATGAAAACA--------------------
Clon_DP2 T--TTCGTG-T-TGGGCGA-CAT------GGATGAAAACA--------------------
Clon_DP4 T--TTCGTG-T-TGGGCGA-CAT------GGATGAAAACA--------------------
Clon_DP10 T--TTCGTG-T-TGGGCGA-CAT------GGATGAAAACA--------------------
Clon_DP5 T--TTCGTG-T-TGGGCGA-CAT------GGATGAAAACA--------------------
Clon_EM4 C--TTTATG-T-TGGGCAA-CAT------GGATAAAGACG--------------------
Clon_EM21 C--TTTATG-T-TGGGCAA-CAT------GGATAAAGACG--------------------
Clon_EM2 C--TTTATG-T-TGGGCAA-CAT------GGATAAAGACG--------------------
Clon_EM23 C--TTTATG-T-TGGGCAA-CAT------GGATAAAGACA--------------------
Clon_EM3 C--TTTACG-T-AGGGCAA-CAT------GGATAAAGACG--------------------
Clon_EM24 C--TTTACG-T-AGGGCAA-CAT------GGATAAAGACG--------------------
Clon_EM22 C--TTTACG-T-AGGGCAA-CAT------GGATAAAGACG--------------------
Clon_EM1 C--TTTATG-T-TGGGCAA-CAT------GGATAAAGACA--------------------
Clon_EM6 C--TTTATG-T-GGGGCAA-CAT------GGATAAAGACA--------------------
Clon_EM5 C--TTTATG-T-GGGGCAA-CAT------GGATAAAGACA--------------------
Clon_GD1 CTGGGAATACTTTGGGCAACCAAAT---ACGAAGTATCCAATATTTTAAAGGATCCAAAC
Clon_GD10 CTGGGAATACTTTGGGCAACCAAAT---ACGAAGTATCCAATATTTTAAAGGATCCAAAC
Clon_GD2 CTGGGAATACTTTGGGCAACCAAAT---ACGAAGTATCCAATATTTTAAAGGATCCAAAC
Clon_GD5 CTGGGAATACTTTGGGCAACCAAAT---ACGAAGTATCCAATATTTTAAAGGATCCAAAC
Clon_GD3 CTGGGAATACTTTGGGCAACCAAAT---ACGAAGTATCCAATATTTTAAAGGATCCAAAC
Clon_GD12 CTGGGAATACTTTGGGCAACCAAAT---ACGAAGTATCCAATATTTTAAAGGATCCAAAC
Clon_GD7 CTGGGAATACTTTGGGCAACCAAAT---ACGAAGTATCCAATATTTTAAAGGATCCAAAC
Clon_GD9 CTGGGAATACTTTGGGCAACCAAAT---ACGAAGTATCCAATATTTTAAAGGATCCAAAC
Clon_GD8 CTGGGAATACTTTGGGCAACCAAAT---ACGAAGTATCCAATATTTTAAAGGATCCAAAC
Clon_GD13 CTGGGAATACTTTGGGCAACCAAAT---ACGAAGTATCCAATATTTTAAAGGATCCAAAC
Clon_LD5 TTGAGAATACTTTGGGCAACCGAATTATACGAAGTATTCAAT----CAAA-------AAT
Clon_LD13 TTGAGAATACTTTGGGCAACCGAATTATACGAAGTATTCAAT----CAAA-------AAT
Clon_LD14 TTGAGAATACTTTGGGCAACCGAATTATACGAAGTATTCAAT----CAAA-------AAT
Clon_LD1 TTGAGAATACTTTGGGCAACCGAATTATACGAAGTATTCAAT----CAAA-------AAT
Clon_LD11 TTGAGAATACTTTGGGCAACCGAATTATACGAAGTATTCAAT----CAAA-------AAT
Clon_LD3 TTGAGAATACTTTGGGCAACCGAATTATACGAAGTATTCAAT----CAAA-------AAT
Clon_LD2 TTGAGAATACTTTGGGCAACCGAATTATACGAAGTATTCAAT----CAAA-------AAT
Clon_LD12 TTGAGAATACTTTGGGCAACCGAATTATACGAAGTATTCAAT----CAAA-------AAT
Clon_LD8 TTGAGAATACTTTGGGCAACCGAATTATACGAAGTATTCAAT----CAAA-------AAT
Clon_LD15 TTGAGAATACTTTGGGCAACCGAATTATACGAAGTATTCAAT----CAAA-------AAT
***
Clon_DM1 -TTAG--------TTAGACTTCAT-------AACAATGG---------------------
Clon_DM21 -TTAG--------TTAGACTTCAT-------AACAATGG---------------------
Clon_DM6 -ATAG--------TTAGACTTCAT-------AACAATGG---------------------
Clon_DM20 -ATAG--------TTAGACTTCAT-------AACAATGG---------------------
Clon_DM9 -TTAG--------TTTGACTTCAT-------AGCAATGG---------------------
Clon_DM12 -TTAG--------TTTGACTTCAT-------AGCAATGG---------------------
Clon_DM7 -TTAG--------TTGGACTTCAT-------AATAATGG---------------------
Clon_DM11 -TTAG--------TTTGACTTCAT-------AGCAATGG---------------------
Clon_DM14 -TTAG--------TTGGACTTCAT-------AATAATGG---------------------
Clon_DMA -GTAG--------TTAGACTTCAT-------AACAATGG---------------------
Clon_DF1 -TTAG--------TTGGACTTCTTT----AAAGCAATGG---------------------
Clon_DF6 -TTAG--------TTGGACTTCTTT----AAAGCAATGG---------------------
Clon_DF4 -TTAG--------TTGGACTTCTTT----AAAGCAATGG---------------------
Clon_DF26 -TTAG--------TTGGACTTCTTT----AAAGCAATGG---------------------
Clon_DF4_50 -TTAG--------TTCAACTTCAT-------AATAATGG---------------------
Clon_DF19 -TTAG--------TTCAACTTCAT-------AATAATGG---------------------
Clon_DF3 -TTAG--------TTCAACTTCAT-------AATAATGG---------------------
Clon_DF5 -TTAG--------TTCAACTTCAT-------AATAATGG---------------------
Clon_DF2 -TTAG--------TTCAACTTCAT-------AATAATGG---------------------
Clon_DF7 -TTAG--------TTAGACTTCTAT----AAAGCAATGG---------------------
Clon_AS15 GCGGC--------TGTTGGTTGAGAAAGATAGCATACAGTGCTG------TAAGCAGACT
Clon_AS14 GCGGC--------TGTTGGTTGAGAAAGATAGCATACAGTGCTG------TAAGCAGACT
Clon_AS20 GCGGC--------TGTTGGTTGAGAAAGATAGCATACAGTGCTG------TAAGCAGACT
Clon_AS13 GCGGC--------TGTTGGTTGAGAAAGATAGCATACAGTGCTG------TAAGCAGACT
Clon_AS10 GCGGC--------TGTTGGTTGAGAAAGATAGCATACAGTGCT-------TAAGCAGACT
Clon_AS11 GCGGC--------TGTTGGTTGAGAAAGATAGCATACAGTGCTG------TAAGCAGACT
Clon_AS2 GCGGC--------TGTTGGTTGAGAAAGATAGCATACAGTGCT-------TAAGCAGACT
Clon_AS12 GCGGC--------TGTTGGTTGAGAAAGATAGCATACAGTGCTG------TAAGCAGACT
Clon_AS1 GCGGC--------TGTTGGTTGAGAAAGATAGCATACAGTGCTG------TAAGCAGACT
Clon_AS16 GCGGC--------TGTTGGTTGAGAAAGATAGCATACAGTGCT-------TAAGCAGACT
Clon_BT8 ---GC--------AATCGATCTA-----ATGGTTTGCGGT------------------CC
Clon_BT9 ---GC--------AATCGATCTA-----ATGGTTTGCGGT------------------CC
Clon_BT16 ---GC--------AATCGATCTA-----ATGGTTTGCGGT------------------CC
Clon_BT3 ---GC--------AATCGATCTA-----ATGGTTTGCGGT------------------CC
Clon_BT14 ---GT--------AATCGATCTA-----ATGGTTTGCGGT------------------CC
Clon_BT17 ---GC--------AATCGATTTA-----ATGGTTTGCGGT------------------CC
Clon_BT13 ---GC--------AATCGATCTA-----ATGGTTTGCGGT------------------CC
Clon_BT1 ---GC--------AATCGATTTA-----ATGGTTTGCGGT------------------CC
Clon_BT10 ---GT--------AATCGATCTA-----ATGGTTTGCGGT------------------CC
Clon_BT15 ---GC--------AATCGATTTA-----ATGGTTTGCGGT------------------CC
Clon_TPA1_20 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TPA1_22 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TPA1_29 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TPA1_28 GTTGT--------GTGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TPA1_26 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TPA1_21 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TPA1_36 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TPA1_27 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TPA1_23 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TPA1_1 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TF22 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TF24 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TF3 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TF2 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TF23 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TF1 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TF4 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TF7 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TF15 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_TF14 GTTGT--------GCGCAATTTA---AGGCCACTTGCATGAG------------TTGCTT
Clon_DP8 -ATGG--------GAGAATTTC-------TTAATTTAGG---------------------
Clon_DP1 -ATGG--------GAGAATTTC-------TTAATTTAGG---------------------
Clon_DP7 -ATGG--------GAGAATTTC-------TTAATTTAGG---------------------
Clon_DP3 -ATGG--------GAGAATTTC-------TTAATTTAGG---------------------
Clon_DP6 -ATGG--------GAGAATTTC-------TTAATTTAGG---------------------
Clon_DP9 -GTGG--------GAGAATTTC-------TTAATTTAGG---------------------
Clon_DP2 -ATGG--------GAGAATTTC-------TTAATTTAGG---------------------
Clon_DP4 -ATGG--------GAGAATTTC-------TTAATTTAGG---------------------
Clon_DP10 -ATGG--------GAGAATTTC-------TGAATTTAGG---------------------
Clon_DP5 -ATGG--------GACAATTTC-------TTAATTTAGG---------------------
Clon_EM4 -TCGG--------GTGAACTTC-------TTAATTT-GG---------------------
Clon_EM21 -TCGG--------GTGAACTTC-------TTAATTT-GG---------------------
Clon_EM2 -TCGG--------GTGAACTTC-------TCAATT--GG---------------------
Clon_EM23 -CCGG--------GTGAACTTC-------TCAATTA-GG---------------------
Clon_EM3 -TCGG--------GTGAACTTC-------TTAATTT-GG---------------------
Clon_EM24 -TCGG--------GTGAACTTC-------TTAATTT-GG---------------------
Clon_EM22 -TCGG--------GTGAACTTC-------TTAATTT-GG---------------------
Clon_EM1 -TCGG--------GTGAACTTC-------TCAATTG-GG---------------------
Clon_EM6 -TCGG--------GTGAACTTC-------TCAATTA-GG---------------------
Clon_EM5 -TCGG--------GTGAACTTC-------TCAATTA-GG---------------------
Clon_GD1 GGTGCTGCTCG--GCC-AATCATGCAAATGTGTAATATTG------------ATTTGTTT
Clon_GD10 GGTGCTGCTCG--GCC-AATCATGCAAATGTGTAATATTG------------ATTTGTTT
Clon_GD2 GGTGCTGCTCG--GCC-AATCATGCAAATGTGTAATATTG------------ATTTGTTT
Clon_GD5 GGTGCTGCTCG--GCC-AATCATGCAAATGTGTAATATTG------------ATTTGTTT
Clon_GD3 GGTGCTGCTCG--GCC-AATCATGCAAATGTGTAATATTG------------ATTTGTTT
Clon_GD12 GGTGCTGCTCG--GCC-AATCATGCAAATGTGTAATATTG------------ATTTGTTT
Clon_GD7 GGTGCTGCTCG--GCC-AATCATGCAAATGTGTAATATTG------------ATTTGTTT
Clon_GD9 GGTGCTGCTCG--GCC-AATCATGCAAATGTGTAATATTG------------ATTTGTTT
Clon_GD8 GGTGCTGCTCG--GCC-AATCATGCAAATGTGTAATATTG------------ATTTGTTT
Clon_GD13 GGTGCTGCTCG--GCC-AATCATGCAAATGTGTAATATTG------------ATTTGTTT
Clon_LD5 GTTGCTGCTTAATGCTTGATTGATGAACGCGCATCACATCACAGAAGTAGACATCGATTC
Clon_LD13 GTTGCTGCTTAATGCTTGATTGATGAACGCGCATCACATCACAGAAGTAGACATCGATTC
Clon_LD14 GTTGCTGCTTAATGCTTGATTGATGAACGCGCATCACATCACAGAAGTAGACATCGATTC
Clon_LD1 GTTGCTGCTTAATGCTTGATTGATGAACGCGCATCACATCACAGAAGTAGACATCGATTC
Clon_LD11 GTTGCTGCTTAATGCTTGATTGATGAACGCGCATCACATCACAGAAGTAGACATCGATTC
Clon_LD3 GTTGCTGCTTAATGCTTGATTGATGAACGCGCATCACATCACAGAAGTAGACATCGATTC
Clon_LD2 GTTGCTGCTTAATGCTTGATTGATGAACGCGCATCACATCACAGAAGTAGACATCGATTC
Clon_LD12 GTTGCTGCTTAATGCTTGATTGATGAACGCGCATCACATCACAGAAGTAGACATCGATTC
Clon_LD8 GTTGCTGCTTAATGCTTGATTGATGAACGC--ATCACATCACAGAAGTAGACATCGATTC
Clon_LD15 GTTGCTGCTTAATGCTTGATTGATGAACGC--ATCACATCACAGAAGTAGACATCGATTC
Clon_DM1 -----AA---------CTTGA------------------CACTTA-----CCGGTGT---
Clon_DM21 -----AA---------CTTGA------------------CACTTA-----CCGGTGT---
Clon_DM6 -----AA---------CTTGA------------------CACTTA-----CCGGTGT---
Clon_DM20 -----AA---------CTTGA------------------CACTTA-----CCGGTGT---
Clon_DM9 -----AA---------CTTGA------------------CACTTA-----CCGGTGT---
Clon_DM12 -----AA---------CTTGA------------------CACTTA-----CCGGTGT---
Clon_DM7 -----AA---------CTTGA------------------CACTTA-----CCGGTGT---
Clon_DM11 -----AA---------CTTGA------------------CACTTA-----CCGGTGT---
Clon_DM14 -----AA---------CTTGA------------------CACTTA-----CCGGTGT---
Clon_DMA -----AA---------CTTGA------------------CACTTA-----CCGGTGT---
Clon_DF1 -----AA---------CTTGA------------------CACTTA-----CCGATGT---
Clon_DF6 -----AA---------CTTGA------------------CACTTA-----CCGATGT---
Clon_DF4 -----AA---------CTTGA------------------CACTTA-----CCGATGT---
Clon_DF26 -----AA---------CTTGA------------------CACTTA-----CCGATGT---
Clon_DF4_50 -----AA---------CTTGA------------------CACTTA-----CCGATGT---
Clon_DF19 -----AA---------CTTGA------------------CACTTA-----CCGATGT---
Clon_DF3 -----AA---------CTTGA------------------CACTTA-----CCGATGT---
Clon_DF5 -----AA---------CTTGA------------------CACTTA-----CCGATGT---
Clon_DF2 -----AA---------CTTGA------------------CACTTA-----CCGATGT---
Clon_DF7 -----AA---------CTTGA------------------CACTTG-----CCTATGT---
Clon_AS15 AACCAAACTGAAGCACCCGTACCAG--GTA--TACCAGCCAATTA-----CCGCAC----
Clon_AS14 AACCAAACTGAAGCACCCGTACCAG--GTA--TACCAGCCAATTA-----CCGCAC----
Clon_AS20 AACCAAACTGAAGCACCCGTACCAG--GTA--TACCAGCCAATTA-----CCGCAC----
Clon_AS13 AACCAAACTGAAGCACCCGTACCAG--GTA--TACCAGCCAATTA-----CCGCAC----
Clon_AS10 AACCAAACTGAAGCACCCGTACCAG--GTA--TACCAGCCAATTA-----TCGCAC----
Clon_AS11 AACCAAACTGAAGCACCCGTACCAG--GTA--TACCAGCCAATTA-----TCGCAC----
Clon_AS2 AACCAAACTGAAGCACCCGTACCAG--GTA--TACCAGCCAATTA-----TCGCAC----
Clon_AS12 AACCAAACTGAAGCACCCGTACCAG--GTA--TACCAGCCAATTA-----CCGCAC----
Clon_AS1 AACCAAACTGAAGCACCCGTACCAGCAGTA--TACCAGCCAATTA-----CCGCAC----
Clon_AS16 AACCAAACTGAAGCACCCGTACCAG--GTA--TACCAGCCAATTA-----CCGCAC----
Clon_BT8 A--CAAGTTAAAA----TGTACT----------------CGATTG-----ACA-------
Clon_BT9 A--CAAGTTAAAA----TGTACT----------------CGATTG-----ACA-------
Clon_BT16 A--CAAGTTAAAA----TGTACT----------------CGATTG-----ACA-------
Clon_BT3 A--CAAGTTAAAA----TGTACT----------------CGATTG-----ACA-------
Clon_BT14 A--CAAGTTAAAA----TGTACT----------------CGATTG-----ACA-------
Clon_BT17 A--CAAGTTAAAA----TGTACT----------------TGATTG-----ACA-------
Clon_BT13 A--CAAGTTAAAA----TGTACT----------------CGATTG-----ACA-------
Clon_BT1 A--CAAGTTAAAA----TGTACT----------------CGATTG-----ACA-------
Clon_BT10 A--CAAGTTAAAA----TGTACT----------------CGATTG-----ACA-------
Clon_BT15 A--CAAGTTAAAA----TGTACT----------------CGATTG-----ACA-------
Clon_TPA1_20 AG---AATT------CTTCCACGAA-------------GCAAGTA-----CCACCATTTG
Clon_TPA1_22 AG---AATT------CTTCCACGAA-------------GCAAGTA-----CCACCATTTG
Clon_TPA1_29 AG---AATT------CTTCCACGAA-------------GCAAGTA-----CCGCCATTTG
Clon_TPA1_28 AG---AATT------CTTCCACGAA-------------GCAAGTA-----CCACCATTTG
Clon_TPA1_26 AG---AATT------TCTTTACAAA-------------GCAAGTA-----CCACCATTTG
Clon_TPA1_21 AG---AATT------CTTCCACGAA-------------GCAAGTA-----CCACCATTTG
Clon_TPA1_36 AG---AATT------CTTCCACGAA-------------GCAAGTA-----CCACCATTTG
Clon_TPA1_27 AG---AATT------CTTCCACGAA-------------GCAAGTA-----CCACCATTTG
Clon_TPA1_23 AG---AATT------CTTCCACGAA-------------GCAAGTA-----CCACCATTTG
Clon_TPA1_1 AG---AATT------CTTCCACGAA-------------GCAAGTA-----CCACCATTTG
Clon_TF22 AG---AA--------CTTCCTTGAAA-----------AGCAGGTG-----T-ACTAACTA
Clon_TF24 AG---AA--------CTTCCTTGAAA-----------AGCAGGTG-----T-ACTAACTA
Clon_TF3 AG---AA--------CTTCCTTGAAA-----------AGCAGGTG-----T-ACTAACTA
Clon_TF2 AG---AA--------CTTCCTTGAAA-----------AGCAGGTG-----T-ACTAACTA
Clon_TF23 AG---AA--------CTTCCTTGAAA-----------AGCAGGTG-----T-ACTAACTA
Clon_TF1 AG---AA--------CTTCCTTGAAA-----------AGCAGGTG-----T-ACTAACTA
Clon_TF4 AG---AA--------CTTCCTTGAAA-----------AGCAGGTG-----T-ACTAACTA
Clon_TF7 AG---AA--------CTTCCTTGAAA-----------AGCAGGTG-----T-ACTAACTA
Clon_TF15 AG---AA--------CTTCCTTGAAA-----------AGCAGGTG-----T-ACTAACTA
Clon_TF14 AG---AA--------CTTCCTTGAAA-----------AGCAGGTG-----T-ACTAACTA
Clon_DP8 -----AG---------CTAAAC-----------------CGA--A-----TCA-TGT---
Clon_DP1 -----AG---------CTAAAC-----------------CGA--A-----TCA-TGT---
Clon_DP7 -----AG---------CTGGAC-----------------CGA--A-----TCA-TGT---
Clon_DP3 -----AG---------CTGGAT-----------------CGA--A-----TCA-TCT---
Clon_DP6 -----AG---------CTGGAT-----------------CGA--A-----TCA-TCT---
Clon_DP9 -----AG---------CTGGAT-----------------CGA--A-----TCA-TCT---
Clon_DP2 -----AG---------CTGGAC-----------------CGA--A-----TCA-TGT---
Clon_DP4 -----AG---------CTGGAC-----------------CGA--A-----TCA-TGT---
Clon_DP10 -----GG---------TTGGAC-----------------CGA--A-----TCA-TGT---
Clon_DP5 -----AG---------TAAGAC-----------------CGA--A-----TCA-TGT---
Clon_EM4 -----AG---------CCTGA------------------CAATTA-----TCAATGTG--
Clon_EM21 -----AG---------CCTGA------------------CAATTA-----TCAATGTG--
Clon_EM2 -----AG---------CCTGA------------------CAATCA-----TCAATGTG--
Clon_EM23 -----AG---------CCTGA------------------CAATTA-----TCAATGTG--
Clon_EM3 -----AG---------CCTGA------------------CAATTA-----TCAATGTG--
Clon_EM24 -----AG---------CCTGA------------------CAATTA-----TCAATGTG--
Clon_EM22 -----AG---------CCTGA------------------CAATTA-----TCAATGTG--
Clon_EM1 -----AG---------CCTGA------------------CAATTA-----TCAATGTG--
Clon_EM6 -----AG---------CCTGA------------------CAATTA-----TCAATGTG--
Clon_EM5 -----AG---------CCTGA------------------CAATTA-----TCAATGTG--
Clon_GD1 ATTCAAGTCGG----CTGGCATT---------------TCACTTA---CCTTGATT--A-
Clon_GD10 ATTCAAGTCGG----CTGGCATT---------------TCACTTA---CCTTGATT--A-
Clon_GD2 ATTCAAGTCGG----CTGGCATT---------------TCACTTA---CCTTGATT--A-
Clon_GD5 ATTCAAGTCGG----CTGGCATT---------------TCACTTA---CCTTGATT--A-
Clon_GD3 ATTCAAGTCGG----CTGGCATT---------------TCACTTA---CCTTGATT--A-
Clon_GD12 ATTCAAGTCGG----CTGGCATT---------------TCACTTA---CCTTGATT--A-
Clon_GD7 ATTCAAGTCGG----CTGGCATT---------------TCACTTA---CCTTGATT--A-
Clon_GD9 ATTCAAGTCGG----CTGGCATT---------------TCACTTA---CCTTGATT--A-
Clon_GD8 ATTCAAGTCGG----CTGGCATT---------------TCACTTA---CCTTGATT--A-
Clon_GD13 ATTCAAGTCGG----CTGGCATT---------------TCACTTA---CCTTGATT--A-
Clon_LD5 AATTGAATCGG----CTGGCATTGCTATGAC------ATTCCATAATTCCTCAATTCCAT
Clon_LD13 AATTGAATCGG----CTGGCATTGCTATGAC------ATTCCATAATTCCTCAATTCCAT
Clon_LD14 AATTGAATCGG----CTGGCATTGCTATGAC------ATTCCATAATTCCTCAATTCCAT
Clon_LD1 AATTGAATCGG----CTGGCATTGCTATGAC------ATTCCATAATTCCTCAATTCCAT
Clon_LD11 AATTGAATCGG----CTGGCATTGCTATGAC------ATTCCATAATTCCTCAATTCCAT
Clon_LD3 AATTGAATCGG----CTGGCATTGCTATGAC------ATTCCATAATTCCTCAATTCCAT
Clon_LD2 AATTGAATCGG----CTGGCATTGCTATGAC------ATTCCATAATTCCTCAATTCCAT
Clon_LD12 AATTGAATCGG----CTGGCATTGCTATGAC------ATTCCATAATTCCTCAATTCCAT
Clon_LD8 AATTGAATCGG----CTGGCATTGCTATGAC------ATTCCATAA--CCTCAATTCCAT
Clon_LD15 AATTGAATCGG----CTGGCATTGCTATGAC------ATTCCATAA--CCTCAATTCCAT
Clon_DM1 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DM21 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DM6 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DM20 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DM9 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DM12 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DM7 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DM11 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DM14 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DMA -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DF1 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DF6 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DF4 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DF26 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DF4_50 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DF19 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DF3 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DF5 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DF2 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_DF7 -----------ACAAC--------------TTTGTCGGCGATTCTC--CTTGCT-ATCTG
Clon_AS15 -----------ACACCAAATGACATGAACTTTCCATGAAAGCTCTCCTTGTATC-ATTCG
Clon_AS14 -----------ACACCAAATGACATGAACTTTCCATGAAAGCTCTCCTTGTATC-ATTCG
Clon_AS20 -----------ACACCAAATGACATGAACTTTCCATGAAAGCTCTCCTTGTATC-ATTCG
Clon_AS13 -----------ACACCAAATGACATGAACTTTCCATGAAAGCTCTCCTTGTATC-ATTCG
Clon_AS10 -----------ACACCAAATGACATGAACTTTCCATGAAAGCTCTCCTTGTATC-ATTCG
Clon_AS11 -----------ACACCAAATGACATGAACTTTCCATGAAAGCTCTCCTTGTATC-ATTCG
Clon_AS2 -----------ACACCAAATGACATGAACTTTCCATGAAAGCTCTCCTTGTATC-ATTCG
Clon_AS12 -----------ACACCAAATGACATGAACTTTCCATGAAAGCTCTCCTTGTATC-ATTCG
Clon_AS1 -----------ACACCAAATGACATGAACTTTCCATGAAAGCTCTCCTTGTATC-ATTCG
Clon_AS16 -----------ACACCAAATGACATGAACTTTCCATGAAAGCTCTCCTTGTATC-ATTCG
Clon_BT8 -----------ACACCA--------------TTTATG------CTTTGTACATC-GTT--
Clon_BT9 -----------ACACCA--------------TTTATG------CTTTGTACATC-GTT--
Clon_BT16 -----------ACACCA--------------TTTATG------CTTTGTACATC-GTT--
Clon_BT3 -----------ACACCA--------------TTTATG------CTTTGTACATC-GTT--
Clon_BT14 -----------ACACCA--------------TTTATG------CTTTGTACATC-GTT--
Clon_BT17 -----------ACACCA--------------TTTATG------CTTTGTACATC-GTT--
Clon_BT13 -----------ACACCA--------------TTTATG------CTTTGTACATC-GTT--
Clon_BT1 -----------ACACCA--------------TTTATG------CTTTGTACATC-GTT--
Clon_BT10 -----------ACACCA--------------TTTATG------CTTTGTACATC-GTT--
Clon_BT15 -----------ACACCA--------------TTTATG------CTTTGTACATC-GTT--
Clon_TPA1_20 AC---------ACAGTACCTCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TPA1_22 AC---------ACAGTACCTCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TPA1_29 AC---------ACAGTACCTCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TPA1_28 AC---------ACAGTACCTCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TPA1_26 AC---------ACAGTACCTCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TPA1_21 AC---------GCAGTACCTCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TPA1_36 AC---------GCAGTACCTCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TPA1_27 AC---------GCAGTACCTCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TPA1_23 AC---------ACAGTACCTCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TPA1_1 AC---------ACAGTACCTCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TF22 GCCA-------ACAGTGC-TCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TF24 GCCA-------ACAGTGC-TCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TF3 GCCA-------ACAGTGC-TCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TF2 GCCA-------ACAGTGC-TCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TF23 GCCA-------ACAGTGC-TCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TF1 GCCA-------ACAGTGC-TCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TF4 GCCA-------ACAGTGC-TCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TF7 GCCA-------ACAGTGC-TCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TF15 GCCA-------ACAGTGC-TCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_TF14 GCCA-------ACAGTGC-TCG-------TCTATCTGAAAGCTCTC--CTTGTCCATCTG
Clon_DP8 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_DP1 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_DP7 ------------TAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_DP3 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_DP6 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_DP9 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_DP2 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_DP4 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_DP10 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_DP5 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_EM4 ------------TAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_EM21 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_EM2 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_EM23 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_EM3 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_EM24 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_EM22 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_EM1 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_EM6 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_EM5 ------------CAAC--------------TTTGTCGGCGATTCTC--CTTGAT-GCCTG
Clon_GD1 -----------ATAATTG-TG------TCGCTTGTTGGCAACTCTGCTCATATC---TTG
Clon_GD10 -----------ATAATTG-TG------TCGCTTGTTGGCAACTCTGCTCATATC---TTG
Clon_GD2 -----------ATAATTG-TG------TCGCTTGTTGGCAACTCTGCTCATATC---TTG
Clon_GD5 -----------ATAATTG-TG------TCGCTTGTTGGCAACTCTGCTCATATC---TTG
Clon_GD3 -----------ATAATTG-TG------TCGCTTGTTGGCAACTCTGCTCATATC---TTG
Clon_GD12 -----------ATAATTG-TG------TCGCTTGTTGGCAACTCTGCTCATATC---TTG
Clon_GD7 -----------ATAATTG-TG------TCGCTTGTTGGCAACTCTGCTCATATC---TTG
Clon_GD9 -----------ATAATTG-TG------TCGCTTGTTGGCAACTCTGCTCATATC---TTG
Clon_GD8 -----------ATAATTG-TG------TCGCTTGTTGGCAACTCTGCTCATATC---TTG
Clon_GD13 -----------ATAATTG-TG------TCGCTTGTTGGCAACTCTGCTCATATC---TTG
Clon_LD5 CTTCATGCGATATGATTGGTGA-----TCGTTTGTTGGCAACTCTGCTCATATC---TTG
Clon_LD13 CTTCATGCGATATGATTGGTGA-----TCGTTTGTTGGCAACTCTGCTCATATC---TTG
Clon_LD14 CTTCATGCGATATGATTGGTGA-----TCGTTTGTTGGCAACTCTGCTCATATC---TTG
Clon_LD1 CTTCATGCGATATGATTGGTGA-----TCGTTTGTTGGCAACTCTGCTCATATC---TTG
Clon_LD11 CTTCATGCGATATGATTGGTGA-----TCGTTTGTTGGCAACTCTGCTCATATC---TTG
Clon_LD3 CTTCATGCGATATGATTGGTGA-----TCGTTTGTTGGCAACTCTGCTCATATC---TTG
Clon_LD2 CTTCATGCGATATGATTGGTGA-----TCGTTTGTTGGCAACTCTGCTCATATC---TTG
Clon_LD12 CTTCATGCGATATGATTGGTGA-----TCGTTTGTTGGCAACTCTGCTCATATC---TTG
Clon_LD8 CTTCATGCGATATGATTGGTGA-----TCGTTTGTTGGCAACTCTGCTCATATC---TTG
Clon_LD15 CTTCATGCGATATGATTGGTGA-----TCGTTTGTTGGCAACTCTGCTCATATC---TTG
* *
Clon_DM1 -T-TGGTGCCTAGTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTGTGGATG
Clon_DM21 -T-TGGTGCCTAGTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTGTGGATG
Clon_DM6 -T-TGGTGCCTAGTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTGTGGATG
Clon_DM20 -T-TGGTGCCTAGTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTGTGGATG
Clon_DM9 -T-TGGTGCCTAGTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTGTGGATG
Clon_DM12 -T-TGGTGCCTAGTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTGTGGATG
Clon_DM7 -T-TGGTGCCTACTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTGTGGATG
Clon_DM11 -T-TGGTGCCTAGTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTGTGGATG
Clon_DM14 -T-TGGTGCCTAGTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTGTGGATG
Clon_DMA -T-TGGTGCCTACTCTACGGTTCCTGTCTA-------TCCTCGGGATGAACGTGTGGATG
Clon_DF1 -T-TGGTGCCTATTCTACGGTTCCTGTTTG-------TCCTCGGGATAAACGTG--GATG
Clon_DF6 -T-TGGTGCCTATTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTG--GATG
Clon_DF4 -T-TGGTGCCTATTCTACGGTTCCTGTTTG-------TCCTCGGGATGAACGTG--GATG
Clon_DF26 -T-TGGTGCCTATTCTACGGTTCCTGTTTG-------TCCTCGGGATGAACGTG--GATG
Clon_DF4_50 -T-TGGTGCCTATTCTACGGTTCCTGTTTG-------TCCTCGGGATAAACGTG--GATG
Clon_DF19 -T-TGGTGCCTATTCTACGGTTCCTGTTTG-------TCCTCGGGATAAACGTG--GATG
Clon_DF3 -T-TGGTGCCTATTCTACGGTTCCTGTTTG-------TCCTCGGGATAAACGTG--GATG
Clon_DF5 -T-TGGTGCCTATTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTG--GATG
Clon_DF2 -T-TGGTGCCTATTCTACGGTTCCTGTTTA-------TCCTCGGGATGAACGTG--GATG
Clon_DF7 -T-TGGTGCCTATTCTACTGTTCCTGTTTG-------TCCTCGGGATAAACGTG--GATG
Clon_AS15 AT-TGGTGCCTAGTCTGCGTTTCCTGCCA-----AATTTTTGGCGT------TG--GATG
Clon_AS14 AT-TGGTGCCTAGTCTGCGTTTCCTGCCA-----AATTTTTGGCGT------TG--GATG
Clon_AS20 AT-TGGTGCCTAGTCTGCGTTTCCTGCCA-----AATTTTTGGCGT------TG--GATG
Clon_AS13 AT-TGGTGCCTAGTCTGCGTTTCCTGCCA-----AATTTTTGGCGT------TG--GATG
Clon_AS10 AT-TGGTGCCTAGTCTGCGTTTCCTGCCA-----AATTTTTGGCGT------TG--GATG
Clon_AS11 AT-TGGTGCCTAGTCTGCGTTTCCTGCCA-----AATTTTTGGCGT------TG--GATG
Clon_AS2 AT-TGGTGCCTAGTCTGCGTTTCCTGCCA-----AATTTTTGGCGT------TG--GATG
Clon_AS12 AT-TGGTGCCTAGTCTGCGTTTCCTGCCA-----AATTTTTGGCGT------TG--GATG
Clon_AS1 AT-TGGTGCCTAGTCTGCGTTTCCTGCCA-----AATTTTTGGCGT------TG--GATG
Clon_AS16 AT-TGGTGCCTAGTCTGCGTTTCCTGCCA-----AATTTTTGGCGT------TG--GATG
Clon_BT8 AT-CGGTGCCTAGTCTGCGGTTC-TGTC---------CTTTAGTGA------TG--GATG
Clon_BT9 AT-CGGTGCCTAGTCTGCGGTTC-TGTC---------CTTTAGTGA------TG--GATG
Clon_BT16 AT-CGGTGCCTAGTCTGCGGTTC-TGTC---------CACTAGTGA------TG--GATG
Clon_BT3 AT-CGGTGCCTAGTCTGCGGTTC-TGTC---------CTTTAGTGA------TG--GATG
Clon_BT14 AT-CGGTGCCTAGTCTGCGGTTC-TGTC---------CTTTAGTGA------TG--GATG
Clon_BT17 AC-CGGTGCCTAGTCTGCGGTTC-TGTC---------CTTTAGTGA------TG--GATG
Clon_BT13 AT-CGGTGCCTAGTCTGCGGTTC-TGTC---------CTTTAGTGA------TG--GATG
Clon_BT1 AC-CGGTGCCTAGTCTGCGGTTC-TGTC---------CTTTAGTGA------TG--GATG
Clon_BT10 AC-CGGTGCCTAGTCTGCGGTTC-TGTC---------CACTAGTGA------TG--GATG
Clon_BT15 AC-CGGTGCCTAGTCTGCGGTTC-TGTC---------CTTTAGTGA------TG--GATG
Clon_TPA1_20 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TCTTAACGGAGGCT-TG--GATG
Clon_TPA1_22 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TCTTAACGGAGGCT-TG--GATG
Clon_TPA1_29 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TCTTAACGGAGGCT-TG--GATG
Clon_TPA1_28 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TCTTAACGGAGGCT-TG--GATG
Clon_TPA1_26 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TCTTAACGGAGGCT-TG--GATG
Clon_TPA1_21 AT-TGGTGCCTAGTCTGCGTTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TPA1_36 AT-TGGTGCCTAGTCTGCGCTTCCTGCT---------TCCTAACGGAGGCT-TG--GATG
Clon_TPA1_27 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TPA1_23 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TPA1_1 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TF22 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TF24 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TF3 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TF2 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TF23 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TF1 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TF4 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TF7 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TF15 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_TF14 AT-TGGTGCCTAGTCTGCGCTTCCTGCC---------TTCTAACAGAGGCT-TG--GATG
Clon_DP8 -T-TGGTGCCTAGCCTACAGTTCCTGTTTG-------TCCTCGGGACAGATGTG--GATG
Clon_DP1 -T-TGGTGCCTAGCCTACAGTTCCTGTTTG-------TCCTCGGGACAGATGTG--GATG
Clon_DP7 -T-TGGTGCCTAGCCTACAGTTCCTGTTTG-------TCCTCGGGACAGATGTG--GATG
Clon_DP3 -T-TGGTGCCTAGCCTACAGTTCCTGTTTG-------TCCTCGGGACAGATGTG--GATG
Clon_DP6 -T-TGGTGCCTAGCCTACAGTTCCTGTTTG-------TCCTCGGGACAGATGTG--GATG
Clon_DP9 -T-TGGTGCCTAGCCTACAGTTCCTGTTTG-------TCCTCGGGACAGATGTG--GATG
Clon_DP2 -T-TGGTGCCTAGCCTACAGTTCCTGTTTG-------TCCTCGGGACAGATGTG--GATG
Clon_DP4 -T-TGGTGCCTAGCCTACAGTTCCTGTTTG-------TCCTCGGGACAGATGTG--GATG
Clon_DP10 -T-TGGTGCCTAGCCTACAGTTCCTGTTTG-------TCCTCGGGACAGATGTG--GATG
Clon_DP5 -T-TGGTGCCTAGCCTACAGTTCCTGTTTG-------TCCTCGGGACAGATGTG--GATG
Clon_EM4 -T-TGGTGCCTAGCCTACAGTTCCTGTCCA-------TCCTCGGGACAGATGTG--GATG
Clon_EM21 -T-TGGTGCCTAGCCTACAGTTCCTGTCCA-------TCCTCGGGACAGATGTG--GATG
Clon_EM2 -T-TGGTGCCTAGCCTACAGTTCCTGTCCA-------TCCTCGGGACAGATGTG--GATG
Clon_EM23 -T-TGGTGCCTAGCCTACAGTTCCTGTCCA-------TCCTCGGGACAGATGTG--GATG
Clon_EM3 -T-TGGTGCCTAGCCTACAGTTCCTGTCCA-------TCCTCGGGACAGATGTG--GATG
Clon_EM24 -T-TGGTGCCTAGCCTACAGTTCCTGTCCA-------TCCTCGGGACAGATGTG--GATG
Clon_EM22 -T-TGGTGCCTAGCCTACAGTTCCTGTCCA-------TCCTCGGGACAGATGTG--GATG
Clon_EM1 -T-TGGTGCCTAGCCTACAGTTCCTGTCCA-------TCCTCGGGACAGATGTG--GATG
Clon_EM6 -T-TGGTGCCTAGCCTACAGTTCCTGTCCA-------TCCTCGGGACAGATGTG--GATG
Clon_EM5 -T-TGGTGCCTAGCCTACAGTTCCTGTCCA-------TCCTCGGGACAGATGTG--GATG
Clon_GD1 TCCTGGTGTCTAGTCCGCTATTCCTAACAA-------AT-TAAAAACTTGTATG--GATG
Clon_GD10 TCCTGGTGTCTAGTCCGCTATTCCTAACAA-------AT-TAAAAACTTGTATG--GATG
Clon_GD2 TTCTGGTGTCTAGTCCGCTATTCCTAACAA-------AT-TAAAAACTTGTATG--GATG
Clon_GD5 TTCTGGTGTCTAGTCCGCTATTCCTAACAA-------AT-TAAAAACTTGTATG--GATG
Clon_GD3 TTCTGGTGTCTAGTCCGCTATTCCTAACAA-------AT-TAAAAACTTGTATG--GATG
Clon_GD12 TTCTGGTGTCTAGTCCGCTATTCCTAACAA-------AT-TAAAAACTTGTATG--GATG
Clon_GD7 TTCTGGTGTCTAGTCCGCTATTCCTAACAA-------AT-TAAAAACTTGTATG--GATG
Clon_GD9 TTCTGGTGTCTAGTCCGCTATTCCTAACAA-------AT-TAAAAACTTGTATG--GATG
Clon_GD8 TTCTGGTGTCTAGTCCGCTATTCCTAACAA-------AT-TAAAAACTTGTATG--GATG
Clon_GD13 TCCTGGTGTCTAGTCCGCTATTCCTAACAA-------AT-TAAAAACTTGTATG--GATG
Clon_LD5 CT-TGGTGCCTAGTCCGCTGTTCCTGCTTT-------CCGTAAGGATTGTCGTG--GATG
Clon_LD13 CT-TGGTGCCTAGTCCGCTGTTCCTGCTTT-------CCGTAAGGATTGTCGTG--GATG
Clon_LD14 CT-TGGTGCCTAGTCCGCTGTTCCTGCTTT-------CCGTAAGGATTGTCGTG--GATG
Clon_LD1 CT-TGGTGCCTAGTCCGCTGTTCCTGCTTT-------CCGTAAGGATTGTCGTG--GATG
Clon_LD11 CT-TGGTGCCTAGTCCGCTGTTCCTGCTTT-------CCGTAAGGATTGTCGTG--GATG
Clon_LD3 CT-TGGTGCCTAGTCCGCTGTTCCTGCTTT-------CCGTAAGGATTGTCGTG--GATG
Clon_LD2 CT-TGGTGCCTAGTCCGCTGTTCCTGCTTT-------CCGTAAGGATTGTCGTG--GATG
Clon_LD12 CT-TGGTGCCTAGTCCGCTGTTCCTGCTTT-------CCGTAAGGATTGTCGTG--GATG
Clon_LD8 CT-TGGTGCCTAGTCCGCTGTTCCTGCTTT-------CCGTAAGGATTGTCGTG--GATG
Clon_LD15 CT-TGGTGCCTAGTCCGCTGTTCCTGCTTT-------CCGTAAGGATTGTCGTG--GATG
**** *** * * *** * * ** ****
Clon_DM1 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DM21 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DM6 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DM20 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DM9 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DM12 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DM7 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DM11 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DM14 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DMA TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DF1 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DF6 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DF4 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DF26 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DF4_50 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DF19 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DF3 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DF5 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DF2 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_DF7 TAG-TGTGTCGCTTG------------TAATGAG----------------TGCCGCTAGG
Clon_AS15 CAG-GGTGTCAGCCAGTTTTGCGACTCTTTTGGG---TTGCTTCTGCTGCTGCT---AGG
Clon_AS14 CAG-GGTGTCAGCCAGTTTTGCGACTCTTTTGGG---TTGCTTCTGCTGCTGCT---AGG
Clon_AS20 CAG-GGTGTCAGCCAGTTTTGCGACTCTTTTGGG---TTGCTTCTGCTGCTGCT---AGG
Clon_AS13 CAG-GGTGTCAGCCAGTTTTGCGACTCTTTTGGG---TTGCTTCTGCTGCTGCT---AGG
Clon_AS10 CAG-GGTGTCAGCCAGTTTTGCGACTCTTTTGGG---TTGCTTCTGCTGCTGCT---AGG
Clon_AS11 CAG-GGTGTCAGCCAGTTTTGCGACTCTTTTGGG---TTGCTTCTGCTGCTGCT---AGG
Clon_AS2 CAG-GGTGTCAGCCAGTTTTGCGACTCTTTTGGG---TTGCTTCTGCTGCTGCT---AGG
Clon_AS12 CAG-GGTGTCAGCCAGTTTTGCGACTCTTTTGGG---TTGCTTCTGCTGCTGCT---AGG
Clon_AS1 CAG-GGTGTCAGCCAGTTTTGCGACTCTTTTGGG---TTGCTTCTGCTGCTGCT---AGG
Clon_AS16 CAG-GGTGTCAGCCA--TTTGCGACTCTTTTGGG---TTGCTTCTGCTGCTGCT---AGG
Clon_BT8 CAG-GGTATCA-------------TTGTATAAGA---T-------------GCT---AGG
Clon_BT9 CAG-GGTATCA-------------TTGTATAAGA---T-------------GCT---AGG
Clon_BT16 CAG-GGTATCA-------------T--TATTAGA---T-------------GCT---AGG
Clon_BT3 CAG-GGTATCA-------------T--TTATAGA---T-------------GCT---AGG
Clon_BT14 CAG-GGTATCA-------------T--TTATAGA---T-------------GCT---AGG
Clon_BT17 CAG-GGTATCA-------------T--TTATAGA---T-------------GCT---AGG
Clon_BT13 CAG-GGTATCA-------------T--TTATAGA---T-------------GCT---AGG
Clon_BT1 CAG-GGTATCA-------------TT--ATTAGA---T-------------GCT---AGG
Clon_BT10 CAG-GGTATCA-------------TTGTATAAGA---T-------------GCT---AGG
Clon_BT15 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCTCTCCACGGCTGCTGCTAGG
Clon_TPA1_20 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCTCTCCACGGCTGCTGCTAGG
Clon_TPA1_22 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCTCTCCACGGCTGCTGCTAGG
Clon_TPA1_29 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCTCTCCACGGCTGCTGCTAGG
Clon_TPA1_28 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCTCTCCACGGCTGCTGCTAGG
Clon_TPA1_26 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCTCTCCACGGCTGCTGCTAGG
Clon_TPA1_21 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCTCTCCACGGCTGCTGCTAGG
Clon_TPA1_36 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCGCTCGGCAGCTGCTGCTAGG
Clon_TPA1_27 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCTCTCCACGGCTGCTGCTAGG
Clon_TPA1_23 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCGCTCGGCAGCTGCTGCTAGG
Clon_TPA1_1 CAG-GGTGTCAGTTGTGTGAGTG-----GCTAAA--CCCCTCTCCACGGCTGCTGCTAGG
Clon_TF22 CAG-GGTGTCAGTTGAGTGTGTGTGGGTGTTAAAGCCTCCACTGCTCAATTGCTGCTAGG
Clon_TF24 CAG-GGTGTCAGTTGAGTGTGTGTGGGTGTTAAAGCCTCCACTGCTCAATTGCTGCTAGG
Clon_TF3 CAG-GGTGTCAGTTGAGTGTGTGTGGGTGTTAAAGCCTCCACTGCTCAATTGCTGCTAGG
Clon_TF2 CAG-GGTGTCAGTTGAGTGTGTGTGGGTGTTAAAGCCTCCACTGCTCAATTGCTGCTAGG
Clon_TF23 CAG-GGTGTCAGTTGAGTGTGTGTGGGTGTTAAAGCCTCCACTGCTCAATTGCTGCTAGG
Clon_TF1 CAG-GGTGTCAGTTGAGTGTGTGTGGGTGTTAAAGCCTCCACTGCTCAATTGCTGCTAGG
Clon_TF4 CAG-GGTGTCAGTTGAGTGTGTGTGGGTGTTAAAGCCTCCACTGCTCAATTGCTGCTAGG
Clon_TF15 CAG-GGTGTCAGTTGAGTGTGTGTGGGTGTTAAAGCCTCCACTGCTCAATTGCTGCTAGG
Clon_TF14 CAG-GGTGTCAGTTGAGTGTGTGTGGGTGTTAAAGCCTCCACTGCTCAATTGCTGCTAGG
Clon_DP8 TAG-TGTGTCGCTT-------------TGTT--------CTTGCAACAAGTGCCGCTAGG
Clon_DP1 TAG-TGTGTCGCTT-------------TGTT--------CTTGCAACAAGTGCCGCTAGG
Clon_DP7 TAG-TGTGTCGCTT-------------TGTT--------CTTGAAACAAGTGCCGCTAGG
Clon_DP3 TAG-TGTGTCGCTT-------------TGTT--------CTTGAAACAAGTGCCGCTAGG
Clon_DP6 TAG-TGTGTCGCTT-------------TGTT--------CTTGAAACAAGTGCCGCTAGG
Clon_DP9 TAG-TGTGTCGCTT-------------TGTT--------CTTGAAACAAGTGCCGCTAGG
Clon_DP2 TAG-TGTGTCGCTT-------------TGTTGTT-----TTTGCAACAAGTGCCGCTAGG
Clon_DP4 TAG-TGTGTCGCTT-------------TGTTGTT-----TTTGCAACAAGTGCCGCTAGG
Clon_DP10 TAG-TGTGTCGCTT-------------TGTTGTT-----TTTGCAACAAGTGCCGCTAGG
Clon_DP5 TAG-TGTGTCGCTT-------------TGTTGTT-----TTTGCAACAAGTGCCGCTAGG
Clon_EM4 TAG-TGTGTCGCTT-------------CAACA---------------AAGTGCCGCTAGG
Clon_EM21 TAG-TGTGTCGCTT-------------CAACA---------------AAGTGCCGCTAGG
Clon_EM2 TAG-TGTGTCGCTT-------------TATCA---------------AAGTGCCGCTAGG
Clon_EM23 TAG-TGTGTCGCTT-------------CATCA---------------AAGTGCCGCTAGG
Clon_EM3 TAG-TGTGTCGCTT-------------CAACA---------------AAGTGCCGCTAGG
Clon_EM24 TAG-TGTGTCGCTT-------------CAACA---------------AAGTGCCGCTAGG
Clon_EM22 TAG-TGTGTCGCTT-------------CAACA---------------AAGTGCCGCTAGG
Clon_EM1 TAG-TGTGTCGCTT-------------CAACA---------------AAGTGCCGCTAGG
Clon_EM6 TAG-TGTGTCGCTT-------------TATCA---------------AAGTGCCGCTAGG
Clon_EM5 TAG-TGTGTCGCTT-------------TATCA---------------AAGTGCCGCTAGG
Clon_GD1 CGG-TGTGTCAGTC-------------TATTGTT-------------GACTGTTGCTAGG
Clon_GD10 CGG-TGTGTCAGTC-------------TATTGTT-------------GACTGTTGCTAGG
Clon_GD2 CGG-TGTGTCAGTC-------------TATTGTT-------------GACTGTTGCTAGG
Clon_GD5 CGG-TGTGTCAGTC-------------TATTGTT-------------GACTGTTGCTAGG
Clon_GD3 CGG-TGTGTCAGTC-------------TATTGTT-------------GACTGTTGCTAGG
Clon_GD12 CGG-TGTGTCAGTC-------------TATTGTT-------------GACTGTTGCTAGG
Clon_GD7 CGG-TGTGTCAGTC-------------TATTGTT-------------GACTGTTGCTAGG
Clon_GD9 CGG-TGTGTCAGTC-------------TATTGTT-------------GACTGTTGCTAGG
Clon_GD8 CGG-TGTGTCAGTC-------------TATTGTT-------------GACTGTTGCTAGG
Clon_GD13 CGG-TGTGTCAGTC-------------TATTGTT-------------GACTGTTGCTAGG
Clon_LD5 CGG-TGTGTCAG-C-------------TAT--AT-------------G-CTGTTGCTAGG
Clon_LD13 CGG-TGTGTCAG-C-------------TAT--AT-------------G-CTGTTGCTAGG
Clon_LD14 CGG-TGTGTCAG-C-------------TAT--AT-------------G-CTGTTGCTAGG
Clon_LD1 CGG-TGTGTCAG-C-------------TAT--AT-------------G-CTGTTGCTAGG
Clon_LD11 CGGGTGTGTCAG-C-------------TAT--AT-------------G-CTGTTGCTAGG
Clon_LD3 CGG-TGTGTCAG-C-------------TAT--AT-------------G-CTGTTGCTAGG
Clon_LD2 CGG-TGTGTCAG-C-------------TAT--AT-------------G-CTGTTGCTAGG
Clon_LD12 CGG-TGTGTCAG-C-------------TAT--AT-------------G-CTGTTGCTAGG
Clon_LD8 CGG-TGTGTCAG-C-------------TAT--AT-------------G-CTGTTGCTAGG
Clon_LD15 CGG-TGTGTCAG-C-------------TAT--AT-------------G-CTGTTGCTAGG
* ** ** * ***
Clon_DM1 TTT-AAATACCAGATTT----GAATGTA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DM21 TTT-AAATACCAAATTT----GAATGTA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DM6 TTT-AAATACCAGATTCCAACAAATATATT-----GTTTGATTGCTGGAGCCACAGGTAC
Clon_DM20 TTT-AAATACCAGATTCCAACAAATATATT-----GTTTGATTGCTGGAGCCACAGGTAC
Clon_DM9 TTT-AAATACCAGATTCCAACAAATATATT-----GTTTGATTGCTGGAGCCACAGGTAC
Clon_DM12 TTT-AAATACCAGATTCCAACAAATATATT-----GTTTGATTGCTGGAGCCACAGGTAC
Clon_DM7 TTT-AAATACCAGATTCCAACAAATATATT-----GTTTGATTGCTGGAGCCACAGGTAC
Clon_DM11 TTT-AAATACCAGATTCCAACAAATATATT-----GTTTGATTGCTGGAGCCACAGGTAC
Clon_DM14 TTT-AAATACCAGATTCCAACAAATATATT-----GTTTGATTGCTGGAGCCACAGGTAC
Clon_DMA TTT-AAATACCAGATTC----GAATGTA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DF1 TTT-AAATACCAGATTC----GAGCATA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DF6 TTT-AAATACCAGATTC----GAGCATA-------ATTCGATTGCTGGAGCCACAGATAC
Clon_DF4 TTT-AAATACCAGATTC----GAGCATA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DF26 TTT-AAATACCAGATTC----GAGCATA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DF4_50 TTT-AAATACCAGATTC----GAGCATA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DF19 TTT-AAATACCAGATTC----GAGCATA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DF3 TTT-AAATACCAGATTC----GAGCATA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DF5 TTT-AAATACCAGATTC----GAGCATA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DF2 TTT-AAATACCAGATTC----GAGCATA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_DF7 TTT-AAATACCAGATTC----GAGCATA-------ATTCGATTGCTGGAGCCACAGGTAC
Clon_AS15 CTTTAAATATC-------------TACG---------CCAAGCGCCGAAGCCTCGAATGC
Clon_AS14 CTT--AATATC-------------TACG---------CCAAGCGCCGAAGCCTCGAATGC
Clon_AS20 CTT-AAATATC-------------TACG---------CCAAGCGCCGAAGCCTCGAATGC
Clon_AS13 CTT-AAATATC-------------TACG---------CCAAGCGCCGAAGCCTCGAATGC
Clon_AS10 CTT-AAATATC-------------TACG---------CCAAGCGCCGAAGCCTCGAATGC
Clon_AS11 CTT-AAATATC-------------TACG---------CCAAGCGCCGAAGCCTCGAATGC
Clon_AS2 CTT-AAATATC-------------TACG---------CCAAGCGCCGAAGCCTCGAATGC
Clon_AS12 CTT-AAATATC-------------TACG---------CCAAGCGCCGAAGCCTCGAATGC
Clon_AS1 CTT-AAATATC-------------TACG---------CCAAGCGCCGAAGCCTCGAATGC
Clon_AS16 CTT-AAATATC-------------TACG---------CCAAGCGCCGAAGCCTCGAATGC
Clon_BT8 CTT-AAATATC----------------A---------TC--GT---GAAGCC--AAGTG-
Clon_BT9 CTT-AAATATC----------------A---------TC--GT---GAAGCC--AAGTG-
Clon_BT16 CTT-AAATATC----------------A---------TTTTGT---GAAGCC--AAGTG-
Clon_BT3 CTT-AAATATC----------------A---------TTTTGT---GAAGCC--AAGTG-
Clon_BT14 CTT-AAATATC----------------A---------TC--AT---GAAGCC--AAGTG-
Clon_BT17 CTT-AAATATC----------------A---------TC--AT---GAAGCC--AAGTG-
Clon_BT13 CTT-AAATATC----------------A---------TTTTGT---GAAGCC--AAGTG-
Clon_BT1 CTT-AAATATC----------------A---------TC--AT---GAAGCC--AATTG-
Clon_BT10 CTT-AAATATC----------------A---------TC--GT---GAAGCC--AAGTG-
Clon_BT15 CTT-AAATATC----------------A---------TC--GT---GAAGCC--AAGTG-
Clon_TPA1_20 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TPA1_22 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TPA1_29 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TPA1_28 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TPA1_26 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TPA1_21 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TPA1_36 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TPA1_27 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TPA1_23 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TPA1_1 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TF22 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TF24 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TF3 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TF2 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TF23 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TF1 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TF4 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TF15 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_TF14 CTT-AAATATCAGTGCCA-----GTGCG---------CTGAGCGCCGAAGCCTCAGATGC
Clon_DP8 TTT-AAATACTGGATTCGT---CATCGA---------ACGAATGCCGGAGCCACAGGTTC
Clon_DP1 TTT-AAATACTGGATTCGT---CATCGA---------ACGAATGCCGGAGCCACAGGTTC
Clon_DP7 TTT-AAATACTGGATTCGT---CATCGA---------ACGAATGCCGGAGCCACAGGTTC
Clon_DP3 TTT-AAATACTGGATTCGT---AATTGA---------ACGAATGCCGGAGCCACAGGTTC
Clon_DP6 TTT-AAATACTGGATTCGT---AATTGA---------ACGAATGCCGGAGCCACAGGTTC
Clon_DP9 TTT-AAATACTGGATTCGT---AATTGA---------ACGAATGCCGGAGCCACAGGTTC
Clon_DP2 TTT-AAATACTGGATTCGT---CATCGT---------ACGAATGCCGGAGCCACAGGTTC
Clon_DP4 TTT-AAATACTGGATTCGT---CATCGT---------ACGAATGCCGGAGCCACAGGTTC
Clon_DP10 TTT-AAATACTGGATTCGT---CATGGA---------ACGAATGCCGGAGCCACAGGTTC
Clon_DP5 TTT-AAATACTGGATTCGT---CATCGA---------ACGAATGCCGGAGCCACAGGTTC
Clon_EM4 TTT-AAATACTGGATTCG----AATTAT---------TCGAATGTCGGAGCCACAGGTAC
Clon_EM21 TTT-AAATACTGGATTCG----AATTAT---------TCGAATGTCGGAGCCACAGGTAC
Clon_EM2 TTT-AAATACTGGATTCG----AATTAT---------TCGAATGTCGGAGCCACAGGTAC
Clon_EM23 TTT-AAATACTGGATTCG----AATTAT---------TCGAATGTCGGAGCCACAGGTAC
Clon_EM3 TTT-AAATACTGGATTCG----AATTAT---------TCGAATGTCGGAGCCACAGGTAC
Clon_EM24 TTT-AAATACTGGATTCG----AATTAT---------TCGAATGTCGGAGCCACAGGTAC
Clon_EM22 TTT-AAATACTGGATTCG----AATTAT---------TCGAATGTCGGAGCCACAGGTAC
Clon_EM1 TTT-AAATACTGGATTCG----AATTAT---------TCGAATGTCGGAGCCACAGGTAC
Clon_EM6 TTT-AAATACTGGATTCG----AATTAT---------TCGAATGTCGGAGCCACAGGTAC
Clon_EM5 TTT-AAATACTGGATTCG----AATTAT---------TCGAATGTCGGAGCCACAGGTAC
Clon_GD1 CTT-AAATATCGTTAG--------CTTAAC----TGTT-----GACGAAGCCGCAAGTAT
Clon_GD10 CTT-AAATATCGTTAG--------CTTAAC----TGTT-----GACGAAGCCGCAAGTAT
Clon_GD2 CTT-AAATATCGTTAG--------CTTAAC----TGTT-----GACGAAGCCGCAAGTAT
Clon_GD5 CTT-AAATATCGTTAG--------CTTAAC----TGTT-----GACGAAGCCGCAAGTAT
Clon_GD3 CTT-AAATATCGTTAG--------CTTAAC----TGTT-----GACGAAGCCGCAAGTAT
Clon_GD12 CTT-AAATATCGTTAG--------CTTAAC----TGTT-----GACGAAGCCGCAAGTAT
Clon_GD7 CTT-AAATATCGTTAG--------CTTAAC----TGTT-----GACGAAGCCGCAAGTAT
Clon_GD9 CTT-AAATATCGTTAG--------CTTAAC----TGTT-----GACGAAGCCGCAAGTAT
Clon_GD8 CTT-AAATATCGTTAG--------CTTAAC----TGTT-----GACGAAGCCGCAAGTAT
Clon_GD13 CTT-AAATATCGTTAG--------CTTAAC----TGTT-----GACGAAGCCGCAAGTAT
Clon_LD5 CTT-AAATATCGTTT---------CGCAAT----TATTTGGGAAACGAAGCCGCAAGTAT
Clon_LD13 CTT-AAATATCGTTT---------CGCAAT----TATTTGGGAAACGAAGCCGCAAGTAT
Clon_LD14 CTT-AAATATCGTTT---------CGCAAT----TATTTGGGAAACGAAGCCGCAAGTAT
Clon_LD1 CTT-AAATATCGTTT---------CGCAAT----TATTTGGGAAACGAAGCCGCAAGTAT
Clon_LD11 CTT-AAATATCGTTT---------CGCAAT----TATTTGGGAAACGAAGCCGCAAGTAT
Clon_LD3 CTT-AAATATCGTTT---------CGCAAT----TATTTGGGAAACGAAGCCGCAAGTAT
Clon_LD2 CTT-AAATATCGTTT---------CGCAAT----TATTTGGGAAACGAAGCCGCAAGTAT
Clon_LD12 CTT-AAATATCGTTT---------CGCAAT----TATTTGGGAAACGAAGCCGCAAGTAT
Clon_LD8 CTT-AAATATCGTTT---------CGCAATATTACAATTGGGATACGGAGCCGCAAGTAT
Clon_LD15 CTT-AAATATCGTTT---------CGCAATATTACAATTGGGATACGGAGCCGCAAGTAT
** **** * **** *
Clon_DM1 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DM21 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DM6 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DM20 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DM9 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DM12 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DM7 GAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DM11 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DM14 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DMA AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DF1 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DF6 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DF4 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DF26 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DF4_50 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DF19 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DF3 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DF5 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DF2 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_DF7 AAGGC------ATTTTCTTTTT-------------------------CATTTCAAAACAA
Clon_AS15 AAGGTT--CTCGCGTACAGTATAC--------CTAGTGTG--CAGTACGTTGA-GTGAGA
Clon_AS14 AAGGTT--CTCGCGTACAGTATAC--------CTAGTGTG--CAGTACGTTGA-GTGAGA
Clon_AS20 AAGGTT--CTCGCGTACAGTATAC--------CTAGTGTG--CAGTACGTTGA-GTGAGA
Clon_AS13 AAGGTT--CTCGCGTACAGCACACA-----CCCAGGTGTGAGCAGTACGTTGA-GTGAGA
Clon_AS10 AAGGTT--CTCGCGTACAGCACACA-----CCCAGGTGTGAGCAGTACGTTGA-GTGAGA
Clon_AS11 AAGGTT--CTCGCGTACAGCACACA-----CCCAGGTGTGAGCAGTACGTTGA-GTGAGA
Clon_AS2 AAGGTT--CTCGCGTACAGCACACA-----CCCAGGTGTGAGCAGTACGTTGA-GTGAGA
Clon_AS12 AAGGTT--CTCGCGTACAGCACACA-----CCTAG-TGTGAGCAGTACGTTGA-GTGAGA
Clon_AS1 AAGGTT--CTCGCGTACAGCACACA-----CCCAGGTGTGAGCAGTACGTTGA-GTGAGA
Clon_AS16 AAGGTT--CTCGCGTACAGCACACA-----CCCAGGTGTGAGCAGTACGTTGA-GTGAGA
Clon_BT8 -----------ATGTACATTA---------------------------GCTAA-GGGAA-
Clon_BT9 -----------ATGTACATTA---------------------------GCTAA-GGGAA-
Clon_BT16 -----------ATGTACATTA---------------------------GCTAA-GGGAA-
Clon_BT3 -----------ATGTACATTA---------------------------GCTAA-GGGAA-
Clon_BT14 -----------ATGTACATTA---------------------------GCTAA-GGGAA-
Clon_BT17 -----------ATGTACATTA---------------------------GCTAA-GGGAA-
Clon_BT13 -----------ATGTACATTA---------------------------GCTAA-GGGAA-
Clon_BT1 -----------ATGTACATTA---------------------------GCTAA-GGGAA-
Clon_BT10 -----------ATGTACATTA---------------------------GCTAA-GGGAA-
Clon_BT15 -----------ATGTACATTA---------------------------GCTAA-GGGAA-
Clon_TPA1_20 AAGGTCGAGTAGTGTGCACTGTTGGGTAAC--CAGCTCT-----GCACGCTGCTGTGAAA
Clon_TPA1_22 AAGGTCGAGTAGTGTGCACTGTTGGGTAAC--CAGCTCT-----GCACGCTGCTGTGAAA
Clon_TPA1_29 AAGGTCGAGTAGTGTGCACTGTTGGGTAAC--CAGCTCT-----GCACGCTGCTGTGAAA
Clon_TPA1_28 AAGGTCGAGTAGTGTGCACTGTTGGGTAAC--CAGCTCT-----GCACGCTGCTGTGAAA
Clon_TPA1_26 AAGGTCGAGTAGTGTGCACTGTTGGGTAAC--CAGCTCT-----GCACGCTGCTGTGAAA
Clon_TPA1_21 AAGGTCGAGTAGTGTGCACTGTTGGGTAAC--CAGCTCT-----GCACGCTGCTGTGAAA
Clon_TPA1_36 AAGGTCGAGTAGTGTGCACTGTTGGGTAAC--CAGCTCT-----GCACGCTGCTGTGAAA
Clon_TPA1_27 AAGGTCGAGTAGTGTGCACTGTTGGGTAAC--CAGCTCT-----GCACGCTGCTGTGAAA
Clon_TPA1_23 AAGGTCGAGTAGTGTGCACTGTTGGGTAAC--CAGCTCT-----GCACGCTGCTGTGAAA
Clon_TPA1_1 AAGGTCGAGTAGTGTGCACTGTTGGGTAAC--CAGCTCT-----GCACGCTGCTGTGAAA
Clon_TF22 AAGGTCGAGTAGTGTGCA--GTTGAGCAAT--CAACG-------GTGCACTGCTGTGAAA
Clon_TF24 AAGGTCGAGTAGTGTGCA--GTTGAGCAAT--CAACG-------GTGCACTGCTGTGAAA
Clon_TF3 AAGGTCGAGTAGTGTGCA--GTTGAGCAAT--CAACG-------GTGCACTGCTGTGAAA
Clon_TF2 AAGGTCGAGTAGTGTGCA--GTTGAGCAAT--CAACG-------GTGCACTGCTGTGAAA
Clon_TF23 AAGGTCGAGTAGTGTGCA--GTTGAGCAAT--CAACG-------GTGCACTGCTGTGAAA
Clon_TF1 AAGGTCGAGTAGTGTGCA--GTTGAGCAAT--CAACG-------GCGCACTGCTGTGAAA
Clon_TF4 AAGGTCGAGTAGTGTGCA--GTTGAGCAAT--CAACG-------GCGCACTGCTGTGAAA
Clon_TF15 AAGGTCGAGTAGTGTGCA--GTTGAGCAAT--CAACG-------GCGCACTGCTGTGAAA
Clon_TF14 AAGGTCGAGTAGTGTGCA--GTTGAGCAAT--CAACG-------GCGCACTGCTGTGAAA
Clon_DP8 AAGGT------ATTTTCTTTTT-------------------------CATTTATGAAAAA
Clon_DP1 AAGGT------ATTTTCTTTTT-------------------------CATTTATGAAAAA
Clon_DP7 AAGGT------ATTTTCTTTTT-------------------------CATTTATGAAAAA
Clon_DP3 AAGGT------ATTTTCTTTTT-------------------------CATTTATGAAAAA
Clon_DP6 AAGGT------ATTTTCTTTTT-------------------------CATTTATGAAAAA
Clon_DP9 AAGGT------ATTTTCTTTTT-------------------------CATTTATGAAAAA
Clon_DP2 AAGGT------ATTTTCTTTTT-------------------------CATTTATGAAAAA
Clon_DP4 AAGGT------ATTTTCTTTTT-------------------------CATTTATGAAAAA
Clon_DP10 AAGGT------ATTTTCTTTTT-------------------------CATTTATGAAAAA
Clon_DP5 AAGGT------ATTTT-TTTTT-------------------------CATTTATGAAAAA
Clon_EM4 AAGGT------ATCT-CTTTTT-------------------------CATTTATGAAAAA
Clon_EM21 AAGGT------ATCT-CTTTTT-------------------------CATTTATGAAAAA
Clon_EM2 AAGGT------ATTT-CTTTTT-------------------------CATTTATGAAAAA
Clon_EM23 AAGGT------ATTT-CTTTTT-------------------------CATTTATGAAAAA
Clon_EM3 AAGGT------ATTT-CTTTTT-------------------------CATTTATGAAAAA
Clon_EM24 AAGGT------ATTT-CTTTTT-------------------------CATTTATGAAAAA
Clon_EM22 AAGGT------ATTT-CTTTTT-------------------------CATTTATGAAAAA
Clon_EM1 AAGGT------ATTT-CTTTTT-------------------------CATTTATGAAAAA
Clon_EM6 GAGGT------ATTT-CTTTTT-------------------------CATTTATGAAAAA
Clon_EM5 GAGGT------ATTT-CTTTTT-------------------------CATTTATGAAAAA
Clon_GD1 GGGGAA----A-CCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_GD10 GGGGAA----A-CCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_GD2 GGGGAA----A-CCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_GD5 GGGGAA----A-CCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_GD3 GGGGAA----A-CCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_GD12 GGGGAA----A-CCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_GD7 GGGGAA----A-CCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_GD9 GGGGAA----A-CCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_GD8 GGGGAA----A-CCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_GD13 GGGGAA----A-CCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_LD5 GGGGAA----AACCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_LD13 GGGGAA----AACCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_LD14 GGGGAA----AACCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_LD1 GGGGAA----AACCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_LD11 GGGGAA----AACCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_LD3 GGGGAA----AACCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_LD2 GGGGAA----AACCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_LD12 GGGGAA----AACCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_LD8 GGGGAA----AACCTTTTCTTT--------------------------ACTTAAACCAAA
Clon_LD15 GGGGAA----AACCTTTTCTTT--------------------------ACTTAAACCAAA
* *
Clon_DM1 CATTGAGCCAGT------------------------------ATC-ATTGA--ATCGTTT
Clon_DM21 CATTGAGCCAGT------------------------------ATC-ATTGA--ATCGTTT
Clon_DM6 CATTGAGCCAGT------------------------------ATC-ATTGA--ATCATTA
Clon_DM20 CATTGAGCCAGT------------------------------ATC-ATTGA--ATCATTA
Clon_DM9 CATTGAGCCAGT------------------------------ATC-ATTGA--ATCATTA
Clon_DM12 CATTGAGCCAGT------------------------------ATC-ATTGA--ATCATTA
Clon_DM7 CATTGAGCCAGT------------------------------ATC-ATTGA--ATCATTA
Clon_DM11 CATTGAGCCAGT------------------------------ATC-ATTGA--ATCGTTT
Clon_DM14 CATTGAGCCAGT------------------------------ATC-ATTGA--ATCGTTT
Clon_DMA CATTGAGCCAGT------------------------------ATC-ATTGA--ATCATTA
Clon_DF1 CATTGAGCCAGT------------------------------ATC-ATTGA--ATCAAGT
Clon_DF6 CATTGAGCCAGT------------------------------ATC-ATTGA--AACAATT
Clon_DF4 CATTGAGCCAGT------------------------------ATC-ATTGA--AACAATT
Clon_DF26 CATTGAGCCAGT------------------------------ATC-ATTGA--AACAATT
Clon_DF4_50 CATTGAGCCAGT------------------------------ATC-ATTGA--AACAATT
Clon_DF19 CATTGAGCCAGT------------------------------ATC-ATTGA--AACAATT
Clon_DF3 CATTGAGCCAGT------------------------------ATC-ATTGA--AACAATT
Clon_DF5 CATTGAGCCAGT------------------------------ATC-ATTGA--AACAATT
Clon_DF2 CATTGAGCCAGT------------------------------ATC-ATTGA--AACAATT
Clon_DF7 CATTGAGCCAGT------------------------------ATC-ATTGA--AACAATT
Clon_AS15 CTGTCTCTGGTCGG-------TTACGGTC-----AAAC-GTAGCC-ACCA---GACGTTC
Clon_AS14 CTGTCTCTGGTCGG-------TTACGGTC-----AAAC-GTAGCC-ACCA---GACGTTC
Clon_AS20 CTGTCTCTGGTCGG-------TTACGGTC-----AAAC GTAGCC-ACCA---GACGTTC
Clon_AS13 CTGTCTCTGGTCGG-------TTACGGTC-----ACAC-GTAACC-ACCACCAGACGTTC
Clon_AS10 CTGTCTCTGGTCGG-------TTACGGTC-----ACAC-GTAACC-ACCACCAGACGTTC
Clon_AS11 CTGTCTCTGGTCGG-------TTACGGTC-----ACAC-GTAACC-ACCACCAGACGTTC
Clon_AS2 CTGTCTCTGGTCGG-------TTGCGGTC-----AAAC-GTAGCC-ACCA---GACGTTC
Clon_AS12 CTGTCTCTGGTCGG-------TTACGGTC-----AAAC-GTAGCC-ACCA---GACGTTC
Clon_AS1 CTGTCTCTGGTCGG-------TTACGGTC-----AAAC-GTAGCC-ACCA---GACGTTC
Clon_AS16 CTGTCTCTGGTCGG-------TTACGGTC-----AAAC-GTAGCCCACCA---GACGTTC
Clon_BT8 -----TGTTAAC---------CCCCGGCCCT---AAACCTCAATGCATTT-----CGATG
Clon_BT9 -----TGTTAAC---------CCCCGGCCCT---AAACCTCAATGCATTT-----CGACG
Clon_BT16 -----TGTAAAC---------CCCCGGCCCT---AAACCTCAATGCATTT-----CGGTG
Clon_BT3 -----TGTTAAC---------CCCCGGCCCT---AAACCTCAATGCATTT-----CGATG
Clon_BT14 -----TGTAAAC---------CCCCGGCCCT---AAACCTCAATGCATTT-----CGATG
Clon_BT17 -----TGTAAAC---------CCCCGGCCCT---AAACCTCAATGCATTT-----CGATG
Clon_BT13 -----TGTAAAC---------CCCCGGCCCT---AAACCTCAATGCATTT-----CGGTG
Clon_BT1 -----TGTAAAC---------CCCCGGCCCT---AAACCTCAATGCATTT-----CGGTG
Clon_BT10 -----TGTTAAC---------CCCCGGCCCT---AAACCTCAATGCATTT-----CGATG
Clon_BT15 -----TGTTAAC---------CCCCGGCCCT---AAACCTCAATGCATTT-----CGATG
Clon_TPA1_20 TATCCAGCGGCT-------------------------------CACGCCG-----C-TTG
Clon_TPA1_22 TATCCAGCGGCT-------------------------------CACGCCG-----C-TTG
Clon_TPA1_29 TATCCAGCGGCT-------------------------------CACGCCG-----C-TTG
Clon_TPA1_28 TATCCAGCGGCT-------------------------------CACGCCG-----C-TTG
Clon_TPA1_26 TATCCAGCGGCT-------------------------------CACGCCG-----C-TTG
Clon_TPA1_21 TATCCAGCGGCT-------------------------------CACGTCG-----C-TTG
Clon_TPA1_36 TATCCAGCGGCT-------------------------------CACGCCG-----C-TTG
Clon_TPA1_27 TATCCAGCGGCT-------------------------------CACGCCG-----C-TTG
Clon_TPA1_23 TATCCAGCGGCT-------------------------------CACGTCG-----C-TTG
Clon_TPA1_1 TATCCAGCGGTT-------------------------------CACGCCG-----T-TTG
Clon_TF22 TATCCAGCGGATGCTGCTGCTTCCCAAATTTT--TGGGTGGCGCACGCCG-----TTTTG
Clon_TF24 TATCCAGCGGATGCTGCTGCTTCCCAAATTTT--TGGGTGGCGCACGCCG-----TTTTG
Clon_TF3 TATCCAGCGGATGCTGCTGCTTCCCAAATTTT--TGTGTGGCGCACGCCG-----TTTTG
Clon_TF2 TATCCAGCGGATGCTGCTGCTCCCCAAATTTT--TGGGTTGCGCACGCCG-----TTTTG
Clon_TF23 TATCCAGCGGATGCTGCTGCTCCCCAAATTTT--TGGGTTGCGCACGCCG-----TTTTG
Clon_TF1 TATCCAGCGGATGCTGCTGCTCTCCGAATTTT--CGGGTGGAGCACGCCG-----TTTTG
Clon_TF4 TATCCAGCGGATGCTGCTGCTCTCCGAATTTT--CGGGTGGAGCACGCCG-----TTTTG
Clon_TF7 TATCCAGCGGATGCTGCTGCTCTCCGAATTTT--CGGGTGGAGCACGCCG-----TTTTG
Clon_TF15 TATCCAGCGGATGCTGCTGCTCTCCGAATTTT--CGGGTGGAGCACGCCG-----TTTTG
Clon_TF14 TATCCAGCGGATGCTGCTGCTTCCCGAATTTT--CGGGTGGCGCACGCCG-----TTTTG
Clon_DP8 CTTCAAGCCAG-------------------------------AAACATTGT-TACAAAAC
Clon_DP1 CTTCAAGCCAG-------------------------------AAACATTGT-TACAAAAC
Clon_DP7 CATTGAGCCAG-------------------------------AAACATCGA-TACAAAGC
Clon_DP3 CATTGAGCCAG-------------------------------AAACATTGA-TACAAAGC
Clon_DP6 CATTGAGCCAG-------------------------------AAACATTGA-TACAAAGC
Clon_DP9 CATTGAGCCAG-------------------------------AAACATTGA-TACAAAGC
Clon_DP2 CATTGAGCCAG-------------------------------AAACATTGT-TACAAACC
Clon_DP4 CATTGAGCCAG-------------------------------AAACATTGT-TACAAACC
Clon_DP10 CATTGAGCCAG-------------------------------AAACATTGT-TACAAACC
Clon_DP5 CTTAAAGCCAG-------------------------------AAACATTGT-TACAAAAC
Clon_EM4 CATTGAGCCAG-------------------------------AATCATTG--TATCATTT
Clon_EM21 CATTGAGCCAG-------------------------------AATCATTG--TATCATTT
Clon_EM2 CATTGAGCCAG-------------------------------AATCATTG--TATCATTG
Clon_EM23 CATTGAGCCAG-------------------------------AATCATTG--TATCATTG
Clon_EM3 CATTGAGCCAG-------------------------------AATCATTG--TATCATTG
Clon_EM24 CATTGAGCCAG-------------------------------AATCATTG--TATCATTG
Clon_EM22 CATTGAGCCAG-------------------------------AATCATTG--TATCATTG
Clon_EM1 CATTGAGCCAG-------------------------------AATCATTG--TATCATTT
Clon_EM6 CATTGAGCCAG-------------------------------AATCATTG--TATCATTG
Clon_EM5 CATTGAGCCAG-------------------------------AATCATTG--TATCATTG
Clon_GD1 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_GD10 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_GD2 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_GD5 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_GD3 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_GD12 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_GD7 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_GD9 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_GD8 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_GD13 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_LD5 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_LD13 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_LD14 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_LD1 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_LD11 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_LD3 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_LD2 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_LD12 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_LD8 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_LD15 CTTTAAGCTAAT-------------------------------ACCATTAA-CATTTATG
Clon_DM1 GAATTGTGATTCT-----GAAAATGAATC----------GCAAGAA--------------
Clon_DM21 GAATTGTGATTCT-----GAAAATGAATC----------GCAAGAA--------------
Clon_DM6 AAATTGTGATTCTCTATAAACGAAGAATC----------GCAAGAA--------------
Clon_DM20 AAATTGTGATTCTCTATAAACGAAGAATC----------GCAAGAA--------------
Clon_DM9 AAATTGTGATTCTCTATAAACGAAGAATC----------GCAAGAA--------------
Clon_DM12 AAATTGTGATTCTCTATAAACGAGGAATC----------GCAAGAA--------------
Clon_DM7 AAATTGTGATTCTCTATAAACGAAGAATC----------GCAAGAA--------------
Clon_DM11 CAATTGTGATTAT-----GAAAATAAATC----------GCAAGAA--------------
Clon_DM14 GAATTGTGATTCT-----GAAAATGAATC----------GCAAGAA--------------
Clon_DMA AAATTGTGATTCTCTATAAACGAAGAATC----------GCAAGAA--------------
Clon_DF1 GAATTGTGATTCT-----GAAAATGAATC----------GCAAGAA--------------
Clon_DF6 GAATTGTGATTCT-----GCAAATGAATC----------GCAAGAA--------------
Clon_DF4 GAATTGTGATTCT-----GCAAATGAATC----------GCATGAA--------------
Clon_DF26 GAATTGTGATTCT-----GCAAATGAATC----------GCATGAA--------------
Clon_DF4_50 GAATTGTGATTCT-----GCAAATGAATC----------GCAAGAA--------------
Clon_DF19 GAATTGTGATTCT-----GCAAATGAATC----------GCAAGAA--------------
Clon_DF3 AAATTGTGATTCT-----GCAAATGAATC----------GCAAGAA--------------
Clon_DF5 GAATTGTGATTCT-----GCAAATGGATC----------GCAAAAA--------------
Clon_DF2 AAATTGTGATTCT-----GCAAATGAATC----------GCAAGAA--------------
Clon_DF7 GAATTGTGATTCT-----GCAAATGGATC----------GCAAAAA--------------
Clon_AS15 AGT-TTTCATTCTCT----AAACTCAACTT-----A--AGCCAAAATCAAAACTGATTAA
Clon_AS14 AGT-TTTCATTCTCT----AAACTCAACTT-----A--AGCCAAAATCAAAACTGATTAA
Clon_AS20 AGT-TTTCATTCTCT----AAACTCAACTT-----A--AGCCAAAATCAAAACTGATTAA
Clon_AS13 AGT-TTTCATTCTCT----AAACTCAACTT-----A--AGCCAAAATCAAAACTGATTAA
Clon_AS10 AGT-TTTCATTCTCT----AAACTCAACTT-----A--AGCCAAAATCAAAACTGATTAA
Clon_AS11 AGT-TTTCATTCTCT----AAACTCAACTT-----A--AGCCAAAATCAAAACTGATTAA
Clon_AS2 AGT-TTTCATTCTCT----AAACTCAACTT-----A--AGCCAAAATCAAAACTGATTAA
Clon_AS12 AGT-TTTCATTCTCT----AAACTCAACTT-----A--AGCCAAAATCAAAACTGATTAA
Clon_AS1 AGT-TTTCATTCTCT----AAACTCAACTT-----A--AGCCAAAATCAAAACTGATTAA
Clon_AS16 AGT-TTTCATTCTCT----AAACTCAACTT-----A--AGCCAAAATCAAAACTGATTAA
Clon_BT8 CAT-TGTTGTATTAT----AACCAAAATTT-----A--AGCTAA----AAAACTAAATAA
Clon_BT9 CAT-TGTTGTATTCT----AACCAAAATTT-----A--AGCTAA----AAAACTAAATAA
Clon_BT16 CAT-TGTTGTACTTT----AACCAAAATTT-----A--AGCTAA----AAAACTAAATAA
Clon_BT3 CAT-TGTTGTATTCT----AACCAAAATTT-----A--AGCTAA----AAAACTAAATAA
Clon_BT14 CAT-TGTTGTATTAT----AACCAAAAAT------A--AGCTAAGA--AAAATAAAACAA
Clon_BT17 CAT-TGTTGTATTAT----AACCAAAAAT------A--AGCTAAGA--AAAATAAAACAA
Clon_BT13 CAT-TGTTGTACTTT----AACCAAAAAT------A--AGCTAAAA--CAAATAAAACAA
Clon_BT1 CAT-TGTTGTACTTT----AACCAAAATTT-----A--AGCTAA----AAAACTAAATAA
Clon_BT10 CAT-TGTTGTATTCT----AACCAAAATTT-----A--AGCTAA----AAAACTAAATAA
Clon_BT15 CAT-TGTTGTATTCT----AACCAAAATTT-----A--AGCTAA----AAAACTAAATAA
Clon_TPA1_20 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCACGA-TGATTTA
Clon_TPA1_22 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCACGA-TGATTTA
Clon_TPA1_29 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCACGA-TGATTTA
Clon_TPA1_28 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCACGA-TGATTTA
Clon_TPA1_26 GGTACTTCATTCTCT----AACCTCAACTT-----ACAAGCCAAAATCACGA-TGATTTA
Clon_TPA1_21 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCACGA-TGATTTA
Clon_TPA1_36 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCACGA-TGATTTA
Clon_TPA1_27 GGTACTTCATTCTCT----AAACCCAACTT-----ACAAGCCAAAATCACGA-TGATTTA
Clon_TPA1_23 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCACGA-TGATTTA
Clon_TPA1_1 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCACGA-TGATTTA
Clon_TF22 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCATGA-TGATTTA
Clon_TF24 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCATGA-TGATTTA
Clon_TF3 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCATGA-TGATTTA
Clon_TF2 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCATGA-TGATTTA
Clon_TF23 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCGAAATCATGA-TGATTTA
Clon_TF1 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCATGA-TGATTTA
Clon_TF4 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCATGA-TGATTTA
Clon_TF7 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCATGA-TGATTTA
Clon_TF15 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCATGA-TGATTTA
Clon_TF14 GGTACTTCATTCTCT----AACCCCAACTT-----ACAAGCCAAAATCATGA-TGATTTA
Clon_DP8 AAATTGTGATTCTCG----AA--AGAATT----------GCAAAAA--------------
Clon_DP1 AAATTGTGATTCTCG----AA--AGAATT----------GCAAAAA--------------
Clon_DP7 AAATTGTGATTCTGC----AA--AGAATT----------GCAAAAA--------------
Clon_DP3 AAATTGTGATTCTGC----AA--AGAATT----------GCAAAAA--------------
Clon_DP6 AAATTGTGATTCTGC----AA--AGAATT----------GCAAAAA--------------
Clon_DP9 AAATTGTGATTCTGC----AA--AGAATT----------GCAAAAA--------------
Clon_DP2 AAATTGTGATTCTGC----AA--AGAATT----------GCAAAAA--------------
Clon_DP4 AAATTGTGGTTCTGC----AA--AGAATT----------GCAAAAA--------------
Clon_DP10 AAATTGTGATTCTAC----AA--AGAATT----------GCAAAAA--------------
Clon_DP5 AAGTTGTGATTCTCG----AA--AGAATT----------GCAATAA--------------
Clon_EM4 AATTTGTGATTCTCT----ACGTAGAATT----------GCAAACA--------------
Clon_EM21 AATTTGTGATTCTCT----ACGTAGAATT----------GCAAACA--------------
Clon_EM2 AATTTGTGATTCTCT----ACGTAGAATT----------GCAAACA--------------
Clon_EM23 AATTTGTGATTCTCT----ACGCAGAATT----------GCAAACA--------------
Clon_EM3 AATTTGTGATTCTCT----ACGTAGAATT----------GCAAACA--------------
Clon_EM24 AATTTGTGATTCTCT----ACGTAGAATT----------GCAAACA--------------
Clon_EM22 AATTTGTGATTCTCT----ACGTAGAATT----------GCAAACA--------------
Clon_EM1 AATTTGTGATTCTCT----ACGTAGAATT----------GCAAACA--------------
Clon_EM6 AATTTGTGATTCTCT----ACGTAGAATT----------GCAAACA--------------
Clon_EM5 AATTTGTGATTCTCT----ACGTAGAATT----------GCAAACA--------------
Clon_GD1 GAATTGT-ATTACCTAT-TGGATGCACTT----GTGCTGATGATAGAGATAC--------
Clon_GD10 GAATTGT-ATTACCTAT-TGGATGCACTT----GTGCTGATGATAGAGATAC--------
Clon_GD2 GAATTGT-ATTACCTAT-TGGATGCACATTCGTGTGCTGATAATAGAGATAC--------
Clon_GD5 GAATTGT-ATTACCTAT-TGGATGCACATTCGTGTGCTGATAATAGAGATAC--------
Clon_GD3 GAATTGT-ATTACCTAT-TGGATGCACTT----GTGCTGATAATAGAGATAC--------
Clon_GD12 GAATTGT-ATTACCTAT-TGGATGCACTT----GTGCTGATAATAGAGATAC--------
Clon_GD7 GAATTGT-ATTACCTAT-TGGATGCACTT----GTGCTGATAATAGAGATAC--------
Clon_GD9 GAATTGT-ATTACCTAT-TGGATGCACTT----GTGCTGATAATAGAGATAC--------
Clon_GD8 GAATTGT-ATTACCTAT-TGGATGCACTT----GTGCTGATAATAGAGATAC--------
Clon_GD13 GAATTGT-ATTACCTAT-TGGATGCACTT----GTGCTGATGATAGAGATAC--------
Clon_LD5 GAATTGT-ATTGTCT---TGAAATANITTT----TAATTATTAAAGCGATGC--------
Clon_LD13 GAATTGT-ATTGTCT---TGAAATANITTT----TAATTATTAAAGCGATGC--------
Clon_LD14 GAATTGT-ATTGTCT---TGAAATANITTT----TAATTATTAAAGCGATGC--------
Clon_LD1 GAATTGT-ATTGTCT---TGAAATANITTT----TAATTATTAAAGCGATGC--------
Clon_LD11 GAATTGT-ATTGTCT---TGAAATANITTT----TAATTATTAAAGCGATGC--------
Clon_LD3 GAATTGT-ATTGTCT---TGAAATANITTT----TAATTATTAAAGAGATGC--------
Clon_LD2 GAATTGT-ATTGTCT---TGAAATANITTT----TAATTATTAAAGCGATGC--------
Clon_LD12 GAATTGT-ATTGTCT---TGAAATANITTT----TAATTATTAAAGAGATGC--------
Clon_LD8 GAATTGT-ATTGTCT---TGAAATANITTT----TAATTATTAAAGCGATGC--------
Clon_LD15 GAATTGT-ATTGTCT---TGAAATANITTT----TAATTATTAAAGCGATGC--------
* *
Clon_DM1 ---------------------------------------------AGCAAAAA-----TT
Clon_DM21 ---------------------------------------------AGCAAAAA-----TT
Clon_DM6 ---------------------------------------------AGCAAAAA-----TT
Clon_DM20 ---------------------------------------------AGCAAAAA-----TT
Clon_DM9 ---------------------------------------------AGCAAAAA-----TT
Clon_DM12 ---------------------------------------------AGCAAAAA-----TT
Clon_DM7 ---------------------------------------------AGCAAAAA-----TT
Clon_DM11 ---------------------------------------------AGCAAAAA-----TT
Clon_DM14 ---------------------------------------------AGCAAAAA-----TT
Clon_DMA ---------------------------------------------AGCAAAAA-----TT
Clon_DF1 ---------------------------------------------AGCAAAAA-----TT
Clon_DF6 ---------------------------------------------AGCAAAAA-----TT
Clon_DF4 ---------------------------------------------AGCAAAAA-----TT
Clon_DF26 ---------------------------------------------AGCAAAAA-----TT
Clon_DF4_50 ---------------------------------------------AGCAAAAA-----TT
Clon_DF19 ---------------------------------------------AGCAAAAA-----TT
Clon_DF3 ---------------------------------------------AGCAAAAA-----TT
Clon_DF5 ---------------------------------------------AGCAAAAA-----TT
Clon_DF2 ---------------------------------------------AGCAAAAA-----TT
Clon_DF7 ---------------------------------------------AGCAAAAA-----TT
Clon_AS15 CC--TTAAATTGTACCCTTA-TGGGTCAA------------TTTATAAAAAGAC--AATT
Clon_AS14 CC--TTAAATTGTACCCTTA-TGGGTCAA------------TTTATAAAAAGAC--AATT
Clon_AS20 CC--TTAAATTGTACCCTTA-TGGGTCAA------------TTTATAAAAAGAC--AATT
Clon_AS13 CC--TTAAATTGTACCCTTA-TGGGTCAA------------TTTATAAAAAGAC--AATT
Clon_AS10 CC--TTAAATTGTACCCTTA-TGGGTCAA------------TTTATAAAAAGAC--AATT
Clon_AS11 CC--TTAAATTGTACCCTTA-TGGGTCAA------------TTTATAAAAAGAC--AATT
Clon_AS2 CC--TTAAATTGTACCCTTA-TGGGTCAA------------TTTATAAAAAGAC--AATT
Clon_AS12 CC--TTAAATTGTACCCTTA-TGGGTCAA------------TTTATAAAAAGAC--AATT
Clon_AS1 CC--TTAAATTGTACCCTTA-TGGGTCAA------------TTTATAAAAAGAC--AATT
Clon_AS16 CC--TTAAATTGT--CCTTA-TCG-TCAA------------TTTATAAAAAGAC--AATT
Clon_BT8 -----CAAA-----------------------------------ACAAAAAAA------T
Clon_BT9 -----CAAA-----------------------------------ACAAAAAAA------T
Clon_BT16 -----CAAA-----------------------------------ACAAAAAAAA--A--T
Clon_BT3 -----CAAA-----------------------------------ACAAAAAAA------T
Clon_BT14 A---TCAAA-----------------------------------ATAAAAAA-------T
Clon_BT17 A---TCAAA-----------------------------------ATAAAAAA-------T
Clon_BT13 A---TCAAA-----------------------------------ATATAAAA-------T
Clon_BT1 -----CAAA-----------------------------------ACAAAAAAA------T
Clon_BT10 -----CAAA-----------------------------------ACAAAAAA-------T
Clon_BT15 -----AACA-----------------------------------A--AAAAA-------T
Clon_TPA1_20 CCCTTTAAGT-GTTACCTAG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TPA1_22 CCCTTTAAGT-GTTACCTAG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TPA1_29 CCCTTTAAGT-GTTACCTAG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TPA1_28 CCCTTTAAGT-GTTACCTAG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TPA1_26 CCCTTTAAGT-GTTACCTCG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TPA1_21 CCCTTTAAGT-GTTACCTCG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TPA1_36 CCCTTTAAGT-GTTACCTAG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TPA1_27 CCCTTTAAGT-GTTACCTCG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TPA1_23 CCCTTTAAGT-GTTACCTCG--TGT--AAA-----------CTTAAACAATGACAGAATT
Clon_TPA1_1 CCCTTTAAGT-GTTACCTCG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TF22 CCCTTTAAGT-TTTACCTTG--TGT--AAG-----------CTTAAACAATGACAAAATT
Clon_TF24 CCCTTTAAGT-TTTACCTTG--TGT--AAG-----------CTTAAACAATGACAAAATT
Clon_TF3 CCCTTTAAGT-TTTACCTTG--TGT--AAG-----------CTTAAACAATGACAAAATT
Clon_TF2 CCCTTTAAGT-TTTACCTTG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TF23 CCCTTTAAGT-TTTACCTTG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TF1 CCCTTTAAGT-TTTACCTTG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TF4 CCCTTTAAGT-TTTACCTTG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TF7 CCCTTTAAGT-TTTACCTTG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TF15 CCCTTTAAGT-TTTACCTTG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_TF14 CCCTTTAAGT-TTTACCTTG--TGT--AAG-----------CTTAAACAATGACAGAATT
Clon_DP8 ----------------------------------------------ACAAAAA-----TA
Clon_DP1 ----------------------------------------------ACAAAAA-----TA
Clon_DP7 ----------------------------------------------ACAAAAA-----TA
Clon_DP3 ----------------------------------------------ACAAAAA-----TA
Clon_DP6 ----------------------------------------------ACAAAAA-----TA
Clon_DP9 ----------------------------------------------ACAAAAA-----TA
Clon_DP2 ----------------------------------------------ACAAAT------TA
Clon_DP4 ----------------------------------------------ACAAAAA-----TA
Clon_DP10 ----------------------------------------------ACAAGAA-----TA
Clon_DP5 ----------------------------------------------ACAAATA-----TA
Clon_EM4 ----------------------------------------------AAAAAAA-----TA
Clon_EM21 ----------------------------------------------AAAAAAA-----TA
Clon_EM2 ----------------------------------------------AAAAAAA-----TA
Clon_EM23 ----------------------------------------------AAAAAA------TA
Clon_EM3 ----------------------------------------------AAAAAAA-----TA
Clon_EM24 ----------------------------------------------AAAAAAA-----TA
Clon_EM22 ----------------------------------------------AAAAAAAA----TA
Clon_EM1 ----------------------------------------------AAAAAAA-----TA
Clon_EM6 ----------------------------------------------AAAAAAA-----TA
Clon_EM5 ----------------------------------------------AAAAAAA-----TA
Clon_GD1 ---------------------------------------------AAAAGAGAT--AAAT
Clon_GD10 ---------------------------------------------AAAAGAGAT--AAAT
Clon_GD2 ---------------------------------------------AAAAGAGAT--AAAT
Clon_GD5 ---------------------------------------------AAAAGAGAT--AAAT
Clon_GD3 ---------------------------------------------AAAAGAGAT--AAAT
Clon_GD12 ---------------------------------------------AAAAGAGAT--AAAT
Clon_GD7 ---------------------------------------------AAAAGAGAT--AAAT
Clon_GD9 ---------------------------------------------AAAAGAGAT--AAAT
Clon_GD8 ---------------------------------------------AAAAGAGAT--AAAT
Clon_GD13 ---------------------------------------------AAAAGAGAT--AAAT
Clon_LD5 ---------------------------------------------AAAAGAGAT--AAAT
Clon_LD13 ---------------------------------------------AAAAGAGAT--AAAT
Clon_LD14 ---------------------------------------------AAAAGAGAT--AAAT
Clon_LD1 ---------------------------------------------AAAAGAGAT--AAAT
Clon_LD11 ---------------------------------------------AAAAGAGAT--AAAT
Clon_LD3 ---------------------------------------------AAAAGAGAT--AAAT
Clon_LD2 ---------------------------------------------AAAAGAGAT--AAAT
Clon_LD12 ---------------------------------------------AAAAGAGAT--AAAT
Clon_LD8 ---------------------------------------------AAAAGAGAT--AAAT
Clon_LD15 ---------------------------------------------AAAAGAGAT--AAAT
*
Clon_DM1 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DM21 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DM6 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DM20 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DM9 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGGTAGCTACGT
Clon_DM12 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DM7 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DM11 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DM14 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DMA ACTACTGCCAGTGGTGGATCACTCGGCCCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DF1 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DF6 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DF4 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DF26 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DF450 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DF19 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DF3 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DF5 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DF2 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_DF7 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGTAGCTAGCTACGT
Clon_AS15 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_AS14 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_AS20 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_AS13 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_AS10 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_AS11 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_AS2 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_AS12 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_AS1 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_AS16 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_BT8 ATAGCTGTTAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_BT9 ACAGCTGTTAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_BT16 ACAGCTGTTAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_BT3 ATAGCTGTTAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_BT14 ATAGCTGTTAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_BT17 ACAGCTGTTAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_BT13 ACAGCTGTTAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_BT1 ACAGCTGTTAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_BT10 ACAGCTGTTAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_BT15 ACAGCTGTTAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TPA1_20 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TPA_22 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TPA1_29 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TPA1_28 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TPA1_26 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TPA1_21 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TPA1_36 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TPA1_27 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TPA1_23 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TPA1_1 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TF22 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TF24 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TF3 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGGACGCAGCTAGCTGCGT
Clon_TF2 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TF23 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TF1 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TF4 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TF7 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TF15 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_TF14 ACAACTGTTAGTGGTGGATCACTCGGCACGCTGATCGAGGAAGAACGCAGCTAGCTGCGT
Clon_DP8 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_DP1 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_DP7 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_DP3 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_DP6 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_DP9 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_DP2 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_DP4 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_DP10 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_DP5 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_EM4 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_EM21 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_EM2 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_EM23 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_EM3 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_EM24 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_EM22 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_EM1 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_EM6 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_EM5 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_GD1 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_GD10 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_GD2 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_GD5 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_GD3 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_GD12 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_GD7 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_GD9 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_GD8 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_GD13 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_LD5 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_LD13 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_LD14 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_LD1 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_LD11 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_LD3 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_LD2 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_LD12 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_LD8 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
Clon_LD15 ACTACTGCCAGTGGTGGATCACTCGGCTCGCTGGTCGAGGAAGAACGCAGCTAGCTGCGT
* *** ****************** ***** ********* *** ** ***** ***
Clon_DM1 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DM21 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DM6 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DM20 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DM9 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DM12 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DM7 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DM11 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DM14 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DMA TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DF1 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DF6 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DF4 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DF26 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DF4_50 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DF19 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DF3 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DF5 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DF2 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DF7 TAATCGGTGTGAAATGCAGGACACTCTGATCACTCGACATTCGAACGCACATTGCAGCCA
Clon_AS15 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_AS14 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_AS20 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_AS13 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_AS10 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_AS11 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_AS2 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_AS12 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_AS1 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_AS16 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_BT8 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCC-
Clon_BT9 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCC-
Clon_BT16 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCC-
Clon_BT3 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCC-
Clon_BT14 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCC-
Clon_BT17 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCC-
Clon_BT13 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCC-
Clon_BT1 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCC-
Clon_BT10 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCC-
Clon_BT15 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCC-
Clon_TPA1_20 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TPA1_22 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TPA1_29 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TPA1_28 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TPA1_26 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TPA1_21 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TPA1_36 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TPA1_27 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TPA1_23 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TPA1_1 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TF22 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TF24 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TF3 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TF2 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TF23 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TF1 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TF4 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TF7 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TF15 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_TF14 TAATCGGTATAAAATGCAGGACATGCCGAATACTCGACTTTCGAACGCATATTGCAGCC-
Clon_DP8 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DP1 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DP7 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DP3 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DP6 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DP9 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DP2 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DP4 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DP10 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_DP5 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_EM4 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_EM21 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_EM2 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_EM23 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_EM3 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_EM24 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_EM22 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_EM1 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_EM6 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_EM5 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_GD1 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_GD10 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_GD2 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_GD5 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_GD3 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_GD12 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_GD7 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_GD9 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_GD8 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_GD13 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD5 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD13 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD5 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD13 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD14 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD1 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD11 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD3 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD2 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD12 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD8 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
Clon_LD15 TAATCGGTGTAAAATGCAGGACACGCCGAGCACTCGACATTCGAACGCACATTGCAGCCA
*** **** * ************ * ** ******* ********** ******* *
Clon_DM1 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA---
Clon_DM21 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA---
Clon_DM6 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA---
Clon_DM20 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA---
Clon_DM9 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA---
Clon_DM12 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA---
Clon_DM7 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA---
Clon_DM11 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA---
Clon_DM14 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA---
Clon_DMA TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA---
Clon_DF1 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA--A
Clon_DF6 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA--A
Clon_DF4 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA--A
Clon_DF26 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA--A
Clon_DF4_50 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA--A
Clon_DF19 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA--A
Clon_DF3 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA--A
Clon_DF5 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA--A
Clon_DF2 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCA--A
Clon_DF7 TTGGATATCCGATGGCTTCGTTTGTCTGAGCGTCGTTATCGAAATTTGA-CAAACCACAA
Clon_AS15 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAC--AAG--CCAAAAA--C
Clon_AS14 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAC--AAG--CCAAAAA--C
Clon_AS20 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAC--AAG--CCAAAAA--C
Clon_AS13 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAC--AAG--CCAAAAA--C
Clon_AS10 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAC--AAG--CCAAAAA--C
Clon_AS11 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAC--AAG--CCAAAAA--C
Clon_AS2 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAC--AAG--CCAAAAA--C
Clon_AS12 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAC--AAG--CCAAAAA--C
Clon_AS1 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAC--AAG--CCAAAAA--C
Clon_AS16 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAC--AAG--CCAAAAA--C
Clon_BT8 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTTGAAATGAAAG--CCACAAA-CC
Clon_BT9 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTTGAAATGAAAG--CCACAAA-CC
Clon_BT16 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTTGAAATGAAAG--CCACAAA-CC
Clon_BT3 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTTGAAATGAAAG--CCACAAA-CC
Clon_BT14 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTTGAAATGAAAG--CCACAAA-CC
Clon_BT17 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTTGAAATGAAAG--CCACAAA-CC
Clon_BT13 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTTGAAATGAAAG--CCACAAA-CC
Clon_BT1 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTTGAAATGAAAG--CCACAAA-CC
Clon_BT10 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTTGAAATGAAAG--CCACAAA-CC
Clon_BT15 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTTGAAATGAAAG--CCACAAA-CC
Clon_TPA1_20 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TPA1_22 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TPA1_29 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TPA1_28 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TPA1_26 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TPA1_21 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TPA1_36 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TPA1_27 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TPA1_23 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TPA1_1 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TF22 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TF24 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TF3 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TF2 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TF23 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TF1 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TF4 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TF7 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TF15 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_TF14 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AAT--ATG--CCAAACA--C
Clon_DP8 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-CCAAACA--A
Clon_DP1 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-CCAAACA--A
Clon_DP7 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-CCAAACA--A
Clon_DP3 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-CCAAACA--A
Clon_DP6 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-CCAAACA--A
Clon_DP9 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-CCAAACA--A
Clon_DP2 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-CCAAACA--A
Clon_DP4 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-CCAAACA--A
Clon_DP10 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-CCAAACA--A
Clon_DP5 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-CCAAACA--A
Clon_EM4 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-C-AAATC--A
Clon_EM21 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-C-AAATC--A
Clon_EM2 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-C-AAATC--A
Clon_EM23 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-C-AAATC--A
Clon_EM3 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-C-AAATC--A
Clon_EM24 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-C-AAATC--A
Clon_EM22 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-C-AAATC--A
Clon_EM1 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-C-AAATC--A
Clon_EM6 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-C-AAATC--A
Clon_EM5 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTTATCAAATTATGA-C-AAATC--A
Clon_GD1 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAA-TCAAACA---
Clon_GD10 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAA-TCAAACA---
Clon_GD2 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAA-TCAAACA---
Clon_GD5 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAA-TCAAACA---
Clon_GD3 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAA-TCAAACA---
Clon_GD12 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAA-TCAAACA---
Clon_GD7 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAA-TCAAACA---
Clon_GD9 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAA-TCAAACA---
Clon_GD8 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAA-TCAAACA---
Clon_GD13 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAA-TCAAACA---
Clon_LD5 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD13 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD5 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD13 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD14 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD1 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD11 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD3 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD2 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD12 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD8 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
Clon_LD15 TTGGTCATACCTTGGCTTCGTTTGTCTGAGCGTCGTT---AATTAATAAATCAAACA---
** * * * **** ********** ****** * *
Clon_DM1 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DM21 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DM6 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DM20 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DM9 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DM12 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DM7 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DM11 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DM14 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DMA AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DF1 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DF6 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DF4 AATGGAATAGATCTGTGTC-------------GTCGTT--------ATTCATTTCGGCG-
Clon_DF26 AATGGAATAGATCTGTGTC-------------GTCGTT--------ATTCATTTCGGCG-
Clon_DF4_50 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DF19 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DF3 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DF5 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DF2 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_DF7 AATGGAATAGATCTGTGTC-------------GTCGCG--------ATTCATTTCGGCG-
Clon_AS15 TAT-CAAAAGGTCCGTTCAC-----------CTTCGTATCCTAACCTCTTGTCGGGTTGA
Clon_AS14 TAT-CAAAAGGTCCGTTCAC-----------CTTCGTATCCTAACCTCTTGTCGGGTTGA
Clon_AS20 TAT-CAAAAGGTCCGTTCAC-----------CTTCGTATCCTAACCTCTTGTCGGGTTGA
Clon_AS13 TAT-CAAAAGGTCCGTTCAC-----------CTTCGTATCCTAACCTCTTGTCGGGTTGA
Clon_AS10 TAT-CAAAAGGTCCGTTCAC-----------CTTCGTATCCTAACCTCTTGTCGGGTTGA
Clon_AS11 TAT-CAAAAGGTCCGTTCAC-----------CTTCGTATCCTAACCTCTTGTCGGGTTGA
Clon_AS2 TAT-CAAAAGGTCCGTTCAC-----------CTTCGTATCCTAACCTCTTGTCGGGTTGA
Clon_AS12 TAT-CAAAAGGTCCGTTCAC-----------CTTCGTATCCTAACCTCTTGTCGGGTTGA
Clon_AS1 TAT-CAAAAGGTCCGTTCAC-----------CTTCGTATCCTAACCTCTTGTCGGGTTGA
Clon_AS16 TAT-CAAAAGGTCCGTTCAC-----------CTTCGTATCCTAACCTCTTGTCGGGTTGA
Clon_BT8 TATATGCAAGACTCGTTCA--------------TCGTGT--TGAA--TTTGTTT--TTAA
Clon_BT9 TATATGCAAGACTCGTTCA--------------TCGTGT--TGAA--TTTGTTT--TTAA
Clon_BT16 TATATGCAAGACTCGTTCA--------------TCGTGT--TGAA--TTTGTTT--TTAA
Clon_BT3 TATATGCAAGACTCGTTCA--------------TCGTGT--TGAA--TTTGTTT--TTAA
Clon_BT14 TATATGCAAGACTCGTTCA--------------TCGTGT--TGAA--TTTGTTT--TTAA
Clon_BT17 TATATGCAAGACTCGTTCA--------------TCGTGT--TGAA--TTTGTTT--TCAA
Clon_BT13 TATATGCAAGACTCGTTCA--------------TCGTGT--TAAACTTTTGTTT--TTAA
Clon_BT1 TATATGCAAGACTCGTTCA--------------TCGTGT--TAAACTTTTGTTT--TTAA
Clon_BT10 TATATGCAAGACTCGTTCA--------------TCGTGT--TGAA--TTTGTTT--TTAA
Clon_BT15 TATATGCAAGACTCGTTCA--------------TCGTGT--CAAA--TTTGTTT--TTAA
Clon_TPA1_20 CATGCA-AAGGCCTGTTCC---------CCATGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TPA1_22 CATGCA-AAGGCCTGTTCC---------CCATGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TPA1_29 TATGCA-AAGGCCTGTTCC---------CCATGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TPA1_28 CATGCA-AAGGCCTGTTCC---------CCATGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TPA1_26 CATGCA-AAGGCCTGTTCC---------CCATGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TPA1_21 CATGCA-AAGGCCTGTTCC---------CCATGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TPA1_36 TATGCA-AAGGCCTGTTCC---------CCATGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TPA1_27 CATGCA-AAGGCCTGTTCC---------CCATGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TPA1_23 CATGCA-AAGGCCTGTTCC---------CCATGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TPA1_1 CATGCA-AAGGCCTGTTCC---------CCATGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TF22 CATGCA-AAGGCCTGCTGCACTTGCGTGCAGTGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TF24 CATGCA-AAGGCCTGCTGCACTTGCGTGCAGTGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TF3 CATGCA-AAGGCCTGCTGCACTTGCGTGCAGTGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TF2 CATGCA-AAGGCCTGCTGCACTTGCGTGCAGTGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TF23 CATGCA-AAGGCCTGCTGCACTTGCGTGCAGTGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TF1 CATGCA-AAGGCCTGCTGCACTTGCGTGCAGTGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TF4 CATGCA-AAGGCCTGCTGCACTTGCGTGCAGTGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TF7 CATGCA-AAGGCCTGCTGCACTTGCGTGCAGTGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TF15 CATGCA-AAGGCCTGCTGCACTTGCGTGCAGTGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_TF14 CATGCA-AAGGCCTGCTGCACTTGCGTGCAGTGTCGTGTC--GATCATTCAGTCA-TCGA
Clon_DP8 AATGGGATAGATCTGTTTC-------------GTCGTG----------CATA-----CG-
Clon_DP1 AATGGGATAGATCTGTTTC-------------GTCGTG----------CATA-----CG-
Clon_DP7 AATGGGATAGATCTGTGTC-------------GTCGTG----------CATA-----CG-
Clon_DP3 AATGGGATAGATCTGTTTC-------------GTCGTG----------CAAA-----CG-
Clon_DP6 AATGGGATAGATCTGTTTC-------------GTCGTG----------CAAA-----CG-
Clon_DP9 AATGGGATAGATCTGTTTC-------------GTCGTG----------CAAA-----CG-
Clon_DP2 AATGGGATAGATCTGTTTC-------------GTCGTG----------CATA-----CG-
Clon_DP4 AATGGGATAGATCTGTTTC-------------GTCGTG----------CATA-----CG-
Clon_DP10 AATGGGATAGATCTGTTTC-------------GTCGTG----------TAAA-----CG-
Clon_DP5 AATGGGATAGATCTGTTTC-------------GTCGTG----------CATA-----CG-
Clon_EM4 AATGGGATAGATCTGTTTC-------------GTCGTGAT----GTGTTAAATTG--CG-
Clon_EM21 AATGGGATAGATCTGTTTC-------------GTCGTGAT----GTGTTAAATTG--CG-
Clon_EM2 AATGGGATAGATCTGTTTC-------------GTCGTGAT----A--TTAAATTG--CG-
Clon_EM23 AATGGGATAGATCTGTTTC-------------GTCGTGAT----GTGTTAAATTG--CG-
Clon_EM3 AATGGGATAGATCTGTTTC-------------GTCGTGAT----G--TTAAATTG--CG-
Clon_EM24 AATGGGATAGATCTGTTTC-------------GTCGTGAT----G--TTAAATTG--CG-
Clon_EM22 AATGGGATAGATCTGTTTC-------------GTCGTGAT----G--TCAAATTG--CG-
Clon_EM1 AATGGGATAGATCTGTTTC-------------GTCGTGAT----GTGTTAAATTG--CG-
Clon_EM6 AATGGGATAGATCTGTTTC-------------GTCGTGAT--------TGAATTG--CG-
Clon_EM5 AATGGGATAGATCTGTTTC-------------GTCGTGAT--------TGAATTG--CG-
Clon_GD1 --T-CAAAAGACCTGTTTG--------------TCGTATTCC--TCGATTA-CTTGAGAA
Clon_GD10 --T-CAAAAGACCTGTTTG--------------TCGTATTCC--TCGATTA-CTTGAGAA
Clon_GD2 --T-CAAAAGACCTGTTTG--------------TCGTATTCC--TCGATTA-CTTGAGAA
Clon_GD5 --T-CAAAAGACCTGTTTG--------------TCGTATTCC--TCGATTA-CTTGAGAA
Clon_GD3 --T-CAAAAGACCTGTTTG--------------TCGTATTCC--TCGATTA-CTTGAGAA
Clon_GD12 --T-CAAAAGACCTGTTTG--------------TCGTATTCC--TCGATTA-CTTGAGAA
Clon_GD7 --T-CAAAAGACCTGTTTG--------------TCGTATTCC--TCGATTA-CTTGAGAA
Clon_GD9 --T-CAAAAGACCTGTTTG--------------TCGTATTCC--TCGATTA-CTTGAGAA
Clon_GD8 --T-CAAAAGACCTGTTTG--------------TCGTATTCC--TCGATTA-CTTGAGAA
Clon_GD13 --T-CAAAAGACCTGTTTG--------------TCGTATTCC--TCGATTA-CTTGAGAA
Clon_LD5 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD13 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD5 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD13 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD14 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD1 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD11 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD3 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD2 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD12 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD8 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
Clon_LD15 --T-CAAAAGACCTGTT----------------TCGTATCTTG-TCGTTTAATTCGATGA
* * ***
Clon_DM1 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DM21 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DM6 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DM20 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DM9 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DM12 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DM7 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DM11 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DM14 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DMA -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DF1 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DF6 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DF4 -TCGTGAGATTATTTCTAAACAT-----------------TTTGAA--TGCTGA------
Clon_DF26 -TCGTGAGATTATTTCTAAACAT-----------------TTTGAA--TGCTGA------
Clon_DF4_50 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DF19 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DF3 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DF5 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DF2 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_DF7 -TCGTGAGATTATTTCTAAACAT-----------------TTCGAA--TGCTGA------
Clon_AS15 TTCGTCGGGCCACCTTTAAACACTACTTTAAAATGTACTCTTGTCA--TTTTAA------
Clon_AS14 TTCGTCGGGCCACCTTTAAACACTACTTTAAAATGTACTCTTGTCA--TTTTAA------
Clon_AS20 TTCGTCGGGCCACCTTTAAACACTACTTTAAAATGTACTCTTGTCA--TTTTAA------
Clon_AS13 TTCGTCGGGCCACCTTTAAACACTACTTTAAAATGTACTCTTGTCA--TTTTAA------
Clon_AS10 TTCGTCGGGCCACCTTTAAACACTACTTTAAAATGTACTCTTGTCA--TTTTAAA-----
Clon_AS11 TTCGTCGGGCCACCTTTAAACACTACTTTAAAATGTACTCTTGTCA--TTTTAA------
Clon_AS2 TTCGTCGGGCCACCTTTAAACACTACTTTAAAATGTACTCTTGTCA--TTTTAA------
Clon_AS12 TTCGTCGGGCCACCTTTAAACACTACTTTAAAATGTACTCTTGTCA--TTTTAAA-----
Clon_AS1 TTCGTCGGGCCACCTTTAAACACTACTTTAAAATGTACTCTTGTCA--TTTTAA------
Clon_AS16 TTCGTCGGGCCACCTTTAAACACTACTTTAAAATGTACTCTTGTCA--TTTTAAA-----
Clon_BT8 CTCGTCGAGTCGCATT---------------AATGT----TTGCCA------AA------
Clon_BT9 CTCGTCGAGTCGCATT---------------AATGT----TTGCCA------AA------
Clon_BT16 CTCGTCGAGTCGCATT---------------AATGT----TTGCCA------AA------
Clon_BT3 CTCGTCGAGTCGCATT---------------AATGT----TTGCCA------AA------
Clon_BT14 CTCGTCGAGTCGCATT---------------AATGT----TTGCCA------AA------
Clon_BT17 CTCGTCGAGTCGCATT---------------AATGT----TTGCCA------AA------
Clon_BT13 CTCGTCGAGTCGCATT---------------AATGT----TTGCCA------AA------
Clon_BT1 CTCGTCGAGTCGCATT---------------AATGT----TTGCCA------AA------
Clon_BT10 CTCGTCGAGTCGCATT---------------AATGT----TTGCCA------AA------
Clon_BT15 CTCGTCGAGTCGCATT---------------AATGT----TTGCCA------AA------
Clon_TPA1_20 CTCGTCAGGCCAC-TTGTAACACTTCTTTCAACT------TTGTTA---TTGAA------
Clon_TPA1_22 CTCGTCAGGCCAC-TTGTAACACTTCTTTCAACT------TTGTTA---TTGAA------
Clon_TPA1_29 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TPA1_28 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TPA1_26 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TPA1_21 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TPA1_36 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TPA1_27 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TPA1_23 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TPA1_1 CTCGTCAGGCCAC-TTGTAACACTTCTTTCAACT------TTGTTA---TTGAA------
Clon_TF22 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TF24 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TF3 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TF2 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TF23 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TF1 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TF4 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TF7 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TF15 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_TF14 CTCGTCAGGCCAC-TTGTAACACTACTTTCAACT------TTGTTA---TTGAA------
Clon_DP8 -TCGTCAGGTCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_DP1 -TCGTCAGGTCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_DP7 -TCGTCAGGTCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_DP3 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_DP6 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_DP9 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_DP2 -TCGTCAGGTCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_DP4 -TCGTCAGGTCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_DP10 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_DP5 -TCGTCAGGTCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_EM4 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_EM21 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_EM2 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_EM23 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_EM3 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_EM24 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_EM22 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_EM1 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_EM6 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_EM5 -TCGTCAGATCATTTCCAAACAT-----------------TTGTTA--TGCTGA------
Clon_GD1 TTCGTCAGGTTCAATTGATACAC--------------TGATTGTCA-ATATTA------T
Clon_GD10 TTCGTCAGGTTCAATTGATACAC--------------TGATTGTCA-ATATTA------T
Clon_GD2 TTCGTCAGGTTCAATTGATACAC--------------TGATTGTCA-ATATTA------T
Clon_GD5 TTCGTCAGGTTCAATTGATACAC--------------TGATTGTCA-ATATTA------T
Clon_GD3 TTCGTCAGGTTCAATTGATACAC--------------TGATTGTCA-ATATTA------T
Clon_GD12 TTCGTCAGGTTCAATTGATACAC--------------TGATTGTCA-ATATTA------T
Clon_GD7 TTCGTCAGGTTCAATTGATACAC--------------TGATTGTCA-ATATTA------T
Clon_GD9 TTCGTCAGGTTCAATTGATACAC--------------TGATTGTCA-ATATTA------T
Clon_GD8 TTCGTCAGGTTCAATTGATACAC--------------TGATTGTCA-ATATTA------T
Clon_GD13 TTCGTCAGGTTCAATTGATACAC--------------TGATTGTCA-ATATTA------T
Clon_LD5 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD13 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD5 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD13 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD14 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD1 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD11 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD3 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD2 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD12 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD8 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
Clon_LD15 TTCGTCGGGTTTAATTGATACACGC------------TGATTGTCACACACTACGAATGT
*** * *
Clon_DM1 --CTCTTTT--TTGGTGTGTGAAGG-----------------------------------
Clon_DM21 --CTCTTTT--TTGGTGTGTGAAGG-----------------------------------
Clon_DM6 --CTCTTTT--TTGGTGTGTGAAGG-----------------------------------
Clon_DM20 --CTCTTTT--TTGGTGTGTGAAGG-----------------------------------
Clon_DM9 --CTCTTTT--TTGGTGTGTGAAGG-----------------------------------
Clon_DM12 --CTCTTTT--TTGGTGTGTGAAGG-----------------------------------
Clon_DM7 --CTCTTTT--TTGGTGTGTGAAGG-----------------------------------
Clon_DM11 --CTCTTTT--TTGGTGTGTGAAGG-----------------------------------
Clon_DM14 --CTCTTTT--TTGGTGTGTGAAGG-----------------------------------
Clon_DMA --CTCTTTT--TTGGTGTGTGAAGG-----------------------------------
Clon_DF1 --CTCTTTTGGTTGGTGTGTGAAGG-----------------------------------
Clon_DF6 --CTCTTTTGGTTGGTGTGTGAAGG-----------------------------------
Clon_DF4 --CTCTTTTGGTTGGTGTGTGAAGG-----------------------------------
Clon_DF26 --CTCTTTTGGTTGGTGTGTGAAGG-----------------------------------
Clon_DF4_50 --CTCTTTTGGTTGGTGTGTGAAGG-----------------------------------
Clon_DF19 --CTCTTTTGGTTGGTGTGTGAAGG-----------------------------------
Clon_DF3 --CTCTTTTGGTTGGTGTGTGAAGG-----------------------------------
Clon_DF5 --CTCTTTTGGTTGGTGTGTGAAGG-----------------------------------
Clon_DF2 --CTCTTTTGGTTGGTGTGTGAAGG-----------------------------------
Clon_DF7 --CTCTTTTGGTTGGTGTGTGAAGG-----------------------------------
Clon_AS15 -TGTGCCCATACGAGCGTAAAGACAG---------------TTAACCA------------
Clon_AS14 -TGTGCCCATACGAGCGTAAAGACAG---------------TTAACCA------------
Clon_AS20 -TGTGCCCATACGAGCGTAAAGACAG---------------TTAACCA------------
Clon_AS13 -TGTGCCCATACGAGCGTAAAGACAG---------------TTAACCA------------
Clon_AS10 -TGTGCCCATACGAGCGTAAAGACAG---------------TTAACCA------------
Clon_AS11 -TGTGCCCATACGAGCGTAAAGACAG---------------TTAACCA------------
Clon_AS2 -TGTGCCCATACGAGCGTAAAGACAG---------------TTAACCA------------
Clon_AS12 -TGTGCCCATACGAGCGTAAAGACAG---------------TTAACCA------------
Clon_AS1 -TGTGCCCATACGAGCGTAAAGACAG---------------TTAACCA------------
Clon_AS16 -TGTGCCCATACGAGCGTAAAGACAG---------------TTAACCA------------
Clon_BT8 ------------AAG----GAGACTTTT-------------TTAAGAA------------
Clon_BT9 ------------AAG----GAGACTTTT-------------TTA--AA------------
Clon_BT16 ------------AAG----GAGAC-----------------TTA--AA------------
Clon_BT3 ------------AAG----GAGAC-----------------TTA--AA------------
Clon_BT14 ------------AAG----GAGAC-----------------TTA--AA------------
Clon_BT17 ------------AAG----GAGAC-----------------TTA--AA------------
Clon_BT13 ------------AAG----GAGAC-----------------TTA--AA------------
Clon_BT1 ------------AAG----GAGACTTT--------------TTAGAAA------------
Clon_BT10 ------------AAG----GAGAC-----------------TTA--AA------------
Clon_BT15 ------------AAG----GAGAC-----------------TTA--AA------------
Clon_TPA1_20 --CTGCCCATACGAGCGTAGGGAGAGAG---CCTA-CCAGTTTGCTGG-------------
Clon_TPA1_22 --CTGCCCATACGAGCGTAGGGAGAGAG---CCTA-CCAGTTTGCTGG-------------
Clon_TPA1_29 --CTGCCCATACGAGCGTAGGGAGAGAG---CCTA-CCAGTTTGCTGG-------------
Clon_TPA1_28 --CTGCCCATACGAGCGTAGGGAGAGAG---CCTA-CCAGTTTGCTGG-------------
Clon_TPA1_26 --CTGCCCATACGAGCGTAGGGAGAGAG---CCTA-CCAGTTTGCTGG-------------
Clon_TPA1_21 --CTGCCCATACGAGCGTAGGGAGAGAG---CCTA-CCAGTTTGCTGG-------------
Clon_TPA1_36 --CTGCCCATACGAGCGTAGGGAGAGAG---CCTA-CCAGTTTGCTGG-------------
Clon_TPA1_27 --CTGCCCATACGAGCGTAGGGAGAGAG---CCTA-CCAGTTTGCTGG-------------
Clon_TPA1_23 --CTGCCCATACGAGCGTAGGGAGAGAG---CCTA-CCAGTTTGCTGG-------------
Clon_TPA1_1 --CTGCCCATACGAGCGTAGGGAGAGAG---CCTA-CCAGTTTGCTGG-------------
Clon_TF22 --ATGCCCATACGAGCGTAAGAAGAGAGTTGCTTA-CCAGTTTGCCGG-------------
Clon_TF24 --ATGCCCATACGAGCGTAAGAAGAGAGTTGCTTA-CCAGTTTGCCGG-------------
Clon_TF3 --ATGCCCATACGAGCGTAAGAAGAGAGTTGCTTA-CCAGTTTGCCGG-------------
Clon_TF2 --ATGCCCATACGAGCGTAAGAAGAGAGTTGCTTA-CCAGTTTGCCGG-------------
Clon_TF23 --ATGCCCATACGAGCGTAAGAAGAGAGTTGCTTA-CCAGTTTGCCGG-------------
Clon_TF1 --ATGCCCATACGAGCGTAAGAAGAGAGTTGCTTA-CCAGTTTGCCGG-------------
Clon_TF4 --ATGCCCATACGAGCGTAAGAAGAGAGTTGCTTA-CCAGTTTGCCGG-------------
Clon_TF7 --ATGCCCATACGAGCGTAAGAAGAGAGTTGCTTA-CCAGTTTGCCGG-------------
Clon_TF15 --ATGCCCATACGAGCGTAAGAAGAGAGTTGCTTA-CCAGTTTGCCGG-------------
Clon_TF14 --ATGCCCATACGAGCGTAAGAAGAGAGTTGCTTA-CCAGTTTGCCGG-------------
Clon_DP8 -----CTTTTGTG---GTGAAGAAGG-----------------------------------
Clon_DP1 -----CTTTTGTG---GTGAAGAAGG-----------------------------------
Clon_DP7 -----CTTTTGTG---GTGAAGAAGG-----------------------------------
Clon_DP3 -----CTTTTGTG---GTGAAGAAGG-----------------------------------
Clon_DP6 -----CTTTTGTG---GTGAAGAAGG-----------------------------------
Clon_DP9 -----CTTTTGTG---GTGAAGAAGG-----------------------------------
Clon_DP2 -----CTTTTGTG---GTGAAGAAGG-----------------------------------
Clon_DP4 -----CTTTTGTG---GTGAAGAAGG-----------------------------------
Clon_DP10 -----CTTTTGTG---GTGAAGAAGG-----------------------------------
Clon_DP5 -----CTTTTGTG---GTGAAGAAGG-----------------------------------
Clon_EM4 -----CTCTTGTG---GTGAAGAAGG-----------------------------------
Clon_EM21 -----CTCTTGTG---GTGAAGAAGG-----------------------------------
Clon_EM2 -----CTCTTGTG---GTGAAGAAGG-----------------------------------
Clon_EM23 -----CTCTTGTG---GTGAAGAAGG-----------------------------------
Clon_EM3 -----CTCTTGTG---GTGAAGAAGG-----------------------------------
Clon_EM24 -----CTCTTGTG---GTGAAGAAGG-----------------------------------
Clon_EM22 -----CTCTTGTG---GTGAAGAAGG-----------------------------------
Clon_EM1 -----CTCTTGTG---GTGAAGAAGG-----------------------------------
Clon_EM6 -----CTCTTGTG---GTGAAGAAGG-----------------------------------
Clon_EM5 -----CTCTTGTG---GTGAAGAAGG-----------------------------------
Clon_GD1 GTACGCCCAAAAATGCGTATTGAAGCTGTTTC----GCATATTGCAACAAACATTAATTT
Clon_GD10 GTACGCCCAAAAATGCGTATTGAAGCTGTTTC----GCATATTGCAACAAACATTAATTT
Clon_GD2 GTACGCCCAAAAATGCGTATTGAAGCTGTTTC----GCATATTGCAACAA---TTAATTT
Clon_GD5 GTACGCCCAAAAATGCGTATTGAAGCTGTTTC----GCATATTGCAACAA---TTAATTT
Clon_GD3 GTACGCCCAAAAATGCGTATTGAAGCTGTTTC----GCATATTGCAACAAACATTAATTT
Clon_GD12 GTACGCCCAAAAATGCGTATTGAAGCTGTTTC----GCATATTGCAACAA---TTAATTT
Clon_GD7 GTACGCCCAAAAATGCGTATTGAAGCTGTTTC----GCATATTGCAACAAACATTAATTT
Clon_GD9 GTACGCCCAAGAATGCGTATTGAAGCTGTTTC----GCATATTGCAACAAACATTAATTT
Clon_GD8 GTACGCCCAAAAATGCGTATTGAAGCTGTTTC----GCATATTGCAACAAACATTAATTT
Clon_GD13 GTACGCCCAAAAATGCGTATTGAAGCTGTTTC----GCATATTGCAACAAACATTAATTT
Clon_LD5 GTATGCCCCAAAATTCGTATCGAAGCTTTATCAA--GCATATTGCATC------------
Clon_LD13 GTATGCCCCAAAATTCGTATCGAAGCTTTATCAA--GCATATTGCATC------------
Clon_LD14 GTATGCCCCAAAATTCGTATCGAAGCTTTATCAA--GCATATTGCATC------------
Clon_LD1 GTATGCCCCAAAATTCGTATCGAAGCTTTATCAA--GCATATTGCATC------------
Clon_LD11 GTATGCCCCAAAATTCGTATCGAAGCTTTATCAA--GCATATTGCATC------------
Clon_LD3 GTATGCCCCAAAATTCGTATCGAAGCTTTATCAA--GCATATTGCATC------------
Clon_LD2 GTATGCCCCAAAATTCGTATCGAAGCTTTATCAA--GCATATTGCATC------------
Clon_LD12 GTATGCCCCAAAATTCGTATCGAAGCTTTATCAA--GCATATTGCATC------------
Clon_LD8 GTATGCCCCAAAATTCGTATCGAAGCTTTATCAATTGCATATTGCATC------------
Clon_LD15 GTATGCCCCAAAATTCGTATCGAAGCTTTATCAATTGCATATTGCATC------------
Clon_DM1 -----------------------CTTTGTA-GC--ACATTCATCA---------------
Clon_DM21 -----------------------CTTTGTA-GC--ACATTCATCA---------------
Clon_DM6 -----------------------CTTTGTA-GC--ACATTCATCA---------------
Clon_DM20 -----------------------CTTTGTA-GC--ACATTCATCA---------------
Clon_DM9 -----------------------CTTTGTA-GC--ACATTCATCA---------------
Clon_DM12 -----------------------CTTTGTA-GC--ACATTCATCA---------------
Clon_DM7 -----------------------CTTTGTA-GC--ACATTCATCA---------------
Clon_DM11 -----------------------CTTTGTA-GC--ACATTCATCA---------------
Clon_DM14 -----------------------CTTTGTA-GC--ACATTCATCA---------------
Clon_DMA -----------------------CTTTGTA-GC--ACATTCATCA---------------
Clon_DF1 -----------------------CTTTGTA-GC--ACATTCATCAC--------------
Clon_DF6 -----------------------CTTTGTA-GC--ACATTCATCAC--------------
Clon_DF4 -----------------------CTTTGTA-GC--ACATTCATCAC--------------
Clon_DF26 -----------------------CTTTGTA-GC--ACATTCATCAC--------------
Clon_DF4_50 -----------------------CTTTGTA-GC--ACATTCATCAC--------------
Clon_DF19 -----------------------CTTTGTA-GC--ACATTCATCAC--------------
Clon_DF3 -----------------------CTTTGTA-GC--ACATTCATCAC--------------
Clon_DF5 -----------------------CTTTGTA-GC--ACATTCATCAC--------------
Clon_DF2 -----------------------CTTTGTA-GC--ACATTCATCAC--------------
Clon_DF7 -----------------------CTTTGTA-GC--ACATTCATCAC--------------
Clon_AS15 ----------TACTGATCTT---TTTTGCGTGCCAATACATGCCT-TCCCCTCACGGAGA
Clon_AS14 ----------TACTGATCTT---TTTTGCGTGCCAATACATGCCT-TCCCCTCACGGAGA
Clon_AS20 ----------TACTGATCTT---TTTTGCGTGCCAATACATGCCT-TCCCCTCACGGAGA
Clon_AS13 ----------TACTGATCTT---TTTTGCGTGCCAATACATGCCT-TCCCCTCACGGAGA
Clon_AS10 ----------TACTGATCTT---TTTTGCGTGCCAATACATGCCT-TCCCCTCACGGAGA
Clon_AS11 ----------TACTGATCTT---TTTTGCGTGCCAATACATGCCT-TCCCCTCACGGAGA
Clon_AS2 ----------TACTGATCTT---TTTTGCGTGCCAATACATGCCT-TCCCCTCACGGAGA
Clon_AS12 ----------TACTGATCTT---TTTTGCGTGCCAATACATGCCT-TCCCCTCACGGAGA
Clon_AS1 ----------TACTGATCTT---TTTTGCGTGCCAATACATGCCT-TCCCCTCACGGAGA
Clon_AS16 ----------TACTGATCTT---TTTTGCGTGCCAATACATGCCT-TCCCCTCACGGAGA
Clon_BT8 ----------TAAG---------TTGTCCGTG---GTATGTAC----------------A
Clon_BT9 ----------TAAG---------TTGTCCGTG---GTATGTAC----------------A
Clon_BT16 ----------TAAG---------TTGTCCGTG---GTATGTAC----------------A
Clon_BT3 ----------TAAG---------TTGTCCGTG---GTATGTAC----------------A
Clon_BT14 ----------TAAG---------TTGTCCGTG---GTATGTAC----------------A
Clon_BT17 ----------TAAG---------TTGTCCGTG---GTATGTAC----------------A
Clon_BT13 ----------TAAG---------TTGTCCGTG---GTATGTAC----------------A
Clon_BT1 ----------TAAG---------TTGTCCGTG---GTATGTAC----------------A
Clon_BT10 ----------TAAG---------TTGTCCGTG---GTATGTAC----------------A
Clon_BT15 ----------TAAG---------TTGTCCGTG---GTATGTAC----------------A
Clon_TPA1_20 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCA--CT-----TGCT
Clon_TPA1_22 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCA--CT-----TGCT
Clon_TPA1_29 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCA--CT-----TGCT
Clon_TPA1_28 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCA--CT-----TGCT
Clon_TPA1_26 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCA--CT-----TGCT
Clon_TPA1_21 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCA--CT-----TGCT
Clon_TPA1_36 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCA--CT-----TGCT
Clon_TPA1_27 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCA--CT-----TGCT
Clon_TPA1_23 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCA--CT-----TGCT
Clon_TPA1_1 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCA--CT-----TGCT
Clon_TF22 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCAGACCA---ATTCT
Clon_TF24 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCAGACCA---ATTCT
Clon_TF3 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCAGACCA---ATTCT
Clon_TF2 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCAGACCA---ATTCT
Clon_TF23 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCAGACCA---ATTCT
Clon_TF1 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCAGACCA---ATTCT
Clon_TF4 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCAGACCA---ATTCT
Clon_TF7 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCAGACCA---ATTCT
Clon_TF15 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCAGACCA---ATTCT
Clon_TF14 ----------TACTCGATT--CACTTTGCGTGT--AGATTTGCCGCAGACCA---ATTCT
Clon_DP8 -----------------------CTTTGTA-GC--ACATTCACT----------------
Clon_DP1 -----------------------CTTTGTA-GC--ACATTCACT----------------
Clon_DP7 -----------------------CTTTGTA-GC--ACATTCACT----------------
Clon_DP3 -----------------------CTTTGTA-GC--ACATTCACT----------------
Clon_DP6 -----------------------CTTTGTA-GC--ACATTCACT----------------
Clon_DP9 -----------------------CTTTGTT-GC--ACATTCACT----------------
Clon_DP2 -----------------------CTTTGTA-GC--ACATTCACT----------------
Clon_DP4 -----------------------CTTTGTA-GC--ACATTCACT----------------
Clon_DP10 -----------------------CTTTGTA-GC--ACATTCACT----------------
Clon_DP5 -----------------------CTTTGTA-GC--ACATTCACT----------------
Clon_EM4 -----------------------CTTTGTC-GC--ACATTCACT----------------
Clon_EM21 -----------------------CTTTGTC-GC--ACATTCACT----------------
Clon_EM2 -----------------------CTTTGTC-GC--ACATTCACT----------------
Clon_EM23 -----------------------CTTTGTC-GC--ACATTCACT----------------
Clon_EM3 -----------------------CTTTGTC-GC--ACATTCACT----------------
Clon_EM24 -----------------------CTTTGTC-GC--ACATTCACT----------------
Clon_EM22 -----------------------CTTTGTC-GC--ACATTCACT----------------
Clon_EM1 -----------------------CTTTGTC-GC--ACATTCACT----------------
Clon_EM6 -----------------------CTTTGTC-GC--ACATTCACT----------------
Clon_EM5 -----------------------CTTTGTC-GC--ACATTCACT----------------
Clon_GD1 GCTTGTTCAATGTTGTGATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_GD10 GTTTGTTCAATGTTGTGATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_GD2 ---TGTTCAATGTTGTGATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_GD5 ---TGTTCAATGTTGTGATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_GD3 GTTTGTTCAATGTTGTGATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_GD12 ---TGTTCAATGTTGTGATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_GD7 GTTTGTTCAATGTTGTGATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_GD9 GTTTGTTCAATGTTGTGATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_GD8 GTTTGTTCAATGTTGTGATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_GD13 GTTTGTTCAATGTTGTGATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_LD5 ----------------GATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_LD13 ----------------GATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_LD14 ----------------GATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_LD1 ----------------GATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_LD11 ----------------GATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_LD3 ----------------GATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_LD2 ----------------GATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_LD12 ----------------GATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_LD8 ----------------GATCGGGTTCTGTGCAC--GCGTTTAATT---------------
Clon_LD15 ----------------GATCGGGTTCTGTGCAC--GCGTTTAATT---------------
* *
Clon_DM1 ------------------GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DM21 ------------------GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DM6 ------------------GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DM20 ------------------GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DM9 ------------------GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DM12 ------------------GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DM7 ------------------GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DM11 ------------------TGGTATTTAGCGTTTC---------------------CAGCT
Clon_DM14 ------------------GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DMA ------------------GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DF1 ------------TAGAA-GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DF6 ------------TAGAA-GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DF4 ------------TAGAA-GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DF26 ------------TAGAA-GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DF4_50 ------------TAGAA-GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DF19 ------------TAGAA-GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DF3 ------------TAAAAAGGGTATTTAGCGTTTC---------------------CAGCT
Clon_DF5 ------------TAGAA-GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DF2 ------------TAGAA-GGGTATTTAGCGTTTC---------------------CAGCT
Clon_DF7 ------------TAGAA-GGGTATTTAGCGTTTC---------------------CAGCT
Clon_AS15 A-GGTATTTGGATGTAGG--GCTTTTGACACTACATGTCAA------------AATGCTT
Clon_AS14 A-GGTATTTGGATGTAGG--GCTTTTGACACTACATGTCAA------------AATGCTT
Clon_AS20 A-GGTATTTGGATGTAGG--GCTTTTGACACTACATGTCAA------------AATGCTT
Clon_AS13 A-GGTATTTGGATGTAGG--GCTTTTGACACTACATGTCAA------------AATGCTT
Clon_AS10 A-GGTATTTGGATGTAGG--GCTTTTGACACTACATGTCAA------------AATGCTT
Clon_AS11 A-GGTATTTGGATGTAGG--GCTTTTGACACTACATGTCAA------------AATGCTT
Clon_AS2 A-GGTATTTGGATGTAGG--GCTTTTGACACTACATGTCAA------------AATGCTT
Clon_AS12 A-GGTATTTGGATGTAGG--GCTTTTGACACTACATGTCAA------------AATGCTT
Clon_AS1 A-GGTATTTGGATGTAGG--GCTTTTGACACTACATGTCAA------------AATGCTT
Clon_AS16 A-GGTATTTGGATGTAGG--GCTTTTGACACTACATGTCAA------------AATGCTT
Clon_BT8 A----------ACGTG------------CATTACA---------------------GATC
Clon_BT9 A----------ACGTG------------CATTACA---------------------GATC
Clon_BT16 A----------ACGTG------------CATTACA---------------------GATC
Clon_BT3 A----------ACGTG------------CATTACA---------------------GATC
Clon_BT14 A----------ACGTG------------CATTACA---------------------GATC
Clon_BT17 A----------ACGTG------------CATTACA---------------------GATC
Clon_BT13 A----------ACGTG------------CATTACA---------------------GATA
Clon_BT1 A----------ACGTG------------CATTACA---------------------GATC
Clon_BT10 A----------ACGTG------------CATTACA---------------------GATC
Clon_BT15 A----------ACGTG------------CATTACA---------------------GATC
Clon_TPA1_20 GTGGTAGTCTAATGTAGGGGGCTTCTGACACTGCCTGTCAGT---------CAAGCACCT
Clon_TPA1_22 GTGGTAGTCTAATGTAGGGGGCTTCTGACACTGCCTGTCAGT---------CAAGCACCT
Clon_TPA1_29 GTGGTAGTCTAATGTAGGGGGCTTCTGACACTGCCTGTCAGT---------CAAGCACCT
Clon_TPA1_28 GTGGTAGTCTAATGTAGGGGGCTTCTGACACTGCCTGTCAGT---------CAAGCACCT
Clon_TPA1_26 GTGGTAGTCTAATGTAGGGGGCTTCTGACACTGCCTGTCAGT---------CAAGCACCT
Clon_TPA1_21 GTGGTAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------CAAGCACCT
Clon_TPA1_36 GTGGTAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------CAAGCACCT
Clon_TPA1_27 GTGGTAGTCTAATGTAGGGGGCTTCTGACACTGCCTGTCAGT---------CAAGCACCT
Clon_TPA1_23 GTGGTAGTCTAATGTAGGGGGCTTCTGACACTGCCTGTCAGT---------CAAGCACCT
Clon_TPA1_1 GTGGTAGTCTAATGTAGGGGGCTTCTGACACTGCCTGTCAGT---------CAAGCACCT
Clon_TF22 GCAGTAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------TTAGCACCT
Clon_TF24 GCAGTAGTCTAATGTAGGGGGCTTCTGACACTATCTGTCAGT---------TTAGCACCT
Clon_TF3 GCGGTAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------TTAGCACCT
Clon_TF2 GCGGTAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------TTAGCACCT
Clon_TF23 GCGGTAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------TTAGCACCT
Clon_TF1 GCGATAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------TTAGCGCCT
Clon_TF4 GCGGTAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------TTAGCACCT
Clon_7F7 GCGGTAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------TTAGCACCT
Clon_7F15 GCGGTAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------TTAGCACCT
Clon_7F14 GCGGTAGTCTAATGTAGGGGGCTTCTGACACTACCTGTCAGT---------TTAGCACCT
Clon_DP8 ------------CACAAGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_DP1 ------------CACAAGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_DP7 ------------CACAAGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_DP3 ------------CACAAAAGGTATTTAGCGTTTC---------------------CAGCT
Clon_DP6 ------------CACAAGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_DP9 ------------CACAAGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_DP2 ------------CACAAAAGGTATTTAGCGTTTC---------------------CAGCT
Clon_DP4 ------------CACAAGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_DP10 ------------CACAAAAGGTATTTAGCGTTTC---------------------CAGCT
Clon_DP5 ------------CACAAGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_EM4 ------------C--AGGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_EM21 ------------C--AGGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_EM2 ------------C--AGGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_EM23 ------------C--AGGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_EM3 ------------C--AGGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_EM24 ------------C--AGGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_EM22 ------------C--AGGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_EM1 ------------C--AGGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_EM6 ------------C--AGGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_EM5 ------------C--AGGAGGTATTTAGCGTTTC---------------------CAGCT
Clon_GD1 -----------ACGC--AGGGCTTTTGGCACATCATGTCAAT-TGCTTGAAATTGCACTA
Clon_GD10 -----------ACGC--AGGGCTTTTGGCACATCATGTCAAT-TGCTTGAAATTGCACTA
Clon_GD2 -----------ACGC--AGGGCTTTTGGCACATCATGTCAAT-TGCTTGAAATTGCACTA
Clon_GD5 -----------ACGC--AGGGCTTTTGGCACATCATGTCAAT-TGCTTGAAATTGCACTA
Clon_GD3 -----------ACGC--AGGGCTTTTGGCACATCATGTCAAT-TGCTTGAAATTGCACTA
Clon_GD12 -----------ACGC--AGGGCTTTTGGCACATCATGTCAAT-TGCTTGAAATTGCACTA
Clon_GD7 -----------ACGC--AGGGCTTTTGGCACATCATGTCAAT-TGCTTGAAATTGCACTA
Clon_GD9 -----------ACGC--AGGGCTTTTGGCACATCATGTCAAT-TGCTTGAAATTGCACTA
Clon_GD8 -----------ACGC--AGGGCTTTTGGCACATCATGTCAAT-TGCTTGAAATTGCACTA
Clon_GD13 -----------ACGC--AGGGCTTTTGGCACATCATGTCAAT-TGCTTGAAATTGCACTA
Clon_LD5 -----------ACGCGCAGGGCTTATGGCACAACATGCCATTATGCCTGGA-TTGCAACA
Clon_LD13 -----------ACGCGCAGGGCTTATGGCACAACATGCCATTATGCCTGGA-TTGCAACA
Clon_LD14 -----------ACGCGCAGGGCTTATGGCACAACATGCCATTATGCCTGGA-TTGCAACA
Clon_LD1 -----------ACGCGCAGGGCTTATGGCACAACATGCCATTATGCCTGGA-TTGCAACA
Clon_LD11 -----------ACGCGCAGGGCTTATGGCACAACATGCCATTATGCCTGGA-TTGCAACA
Clon_LD3 -----------ACGCGCAGGGCTTATGGCACAACATGCCATTATGCCTGGA-TTGCAACA
Clon_LD2 -----------ACGCGCAGGGCTTATGGCACAACATGCCATTATGCCTGGA-TTGCAACA
Clon_LD12 -----------ACGCGCAGGGCTTATGGCACAACATGCCATTATGCCTGGA-TTGCAACA
Clon_LD8 -----------ACGCGCAGGGCTTATGGCACAACATGCCATTATGCCTGGA-TTGCAACA
Clon_LD15 -----------ACGCGCAGGGCTTATGGCACAACATGCCATTATGCCTGGA-TTGCAACA
*
Clon_DM1 AAACAACCCAG----------------------------AATGTGTGC--------CTTG
Clon_DM21 AAACAACCCAG----------------------------AATGTGTGC--------CTTG
Clon_DM6 AAACAACCCAG----------------------------AATGTGTGC--------CTTG
Clon_DM20 AAACAACCCAG----------------------------AATGTGTGC--------CTTG
Clon_DM9 AAACAACCCAG----------------------------AATGTGTGC--------CTTG
Clon_DM12 AAACAACCCAG----------------------------AATGTGTGC--------CTTG
Clon_DM7 AAACAACCCAG----------------------------AATGTGTGC--------CTTG
Clon_DM11 AAACAACCCAG----------------------------AATGTGTGC--------CTTG
Clon_DM14 AAACAACCCAG----------------------------AATGTGTGC--------CTTG
Clon_DMA AAACAACCCAG----------------------------AATGTGTGC--------CTTG
Clon_DF1 AAACAACCCAG----------------------------AATGTGTGC--------CATG
Clon_DF6 AAACAACCCAG----------------------------AATGTGTGC--------CATG
Clon_DF4 AAACAACCCAG----------------------------AATGTGTGC--------CATG
Clon_DF26 AAACAACCCAG----------------------------AATGTGTGC--------CATG
Clon_DF4_50 AAACAACCCAG----------------------------AATGTGTGC--------CATG
Clon_DF19 AAACAACCCAG----------------------------AATGTGTGC--------CATG
Clon_DF3 AAACAACCCAG----------------------------AATGTGTGC--------CATG
Clon_DF5 AAACAACCCAG----------------------------AATGTGTGC--------CATG
Clon_DF2 AAACAACCCAG----------------------------AATGTGTGC--------CATG
Clon_DF7 AAACAACCCAG----------------------------AATGTGTGC--------CATG
Clon_AS15 GAGCAATTAGATCGGGTACCATCTTCT-AGGTG-GAACCAATGTATGT-----TTGCCTT
Clon_AS14 GAGCAATTAGATCGGGTACCATCTTCT-AGGTG-GAACCAATGTATGT-----TTGCCTT
Clon_AS20 GAGCAATTAGATCGGGTACCATCTTCTTAGGTG-GAACCAATGTATGT-----TTGCCTT
Clon_AS13 GAGCAATTAGATCGGGTACCATCTTCTTAGGTG-GAACCAATGTATGT-----TTGCCTT
Clon_AS10 GAGCAATTAGATCGGGTACCATCTTCTTAGGTG-GAACCAATGTATGT-----TTGCCTT
Clon_AS11 GAGCAATTAGATCGGGTACCATCTTCTTAGGTG-GAACCAATGTATGT-----TTGCCTT
Clon_AS2 GAGCAATTAGATCGGGTACCATCTTCT-AGGTG-GAACCAATGTATGT-----TTGCCTT
Clon_AS12 GAGCAATTAGATCGGGTACCATCTTCTTAGGTG-GAACCAATGTATGT-----TTGCCTT
Clon_AS1 GAGCAATTAGATCGGGTACCATCTTCT-AGGTG-GAACCAATGTATGT-----TTGCCTT
Clon_AS16 GAGCAATTAGATCGGGTACCATCTTCTTAGGTG-GAACCAATGTATGT-----TTGCCTT
Clon_BT8 GGACAATTGAA--------CATT-----------------ATGTATGT-----TCACTT-
Clon_BT9 GGACAATTGAA--------CATT-----------------ATGTATGT-----TCACTT-
Clon_BT16 GGACAATTGAA--------CATT-----------------ATGTATGT-----TCACTT-
Clon_BT3 GGACAATTGAA--------CATT-----------------ATGTATGT-----TCACTT-
Clon_BT14 GGACAATTGAA--------CATT-----------------ATGTATGT-----TCACTT-
Clon_BT17 GGACAATTGAA--------CATT-----------------ATGTATGT-----TCACTT-
Clon_BT13 GGACT-TTGAA--------CATT-----------------A---ATGT-----TCACTT-
Clon_BT1 GGACAATTGAA--------CATT-----------------ATGTATGT-----TCACTT-
Clon_BT10 GGACT-TTGAA--------CATT-----------------A---ATGT-----TCACTT-
Clon_BT15 GGACAATTGAA--------CATT-----------------ATGTATAT-----TCACTT-
Clon_TPA1_20 GAGCACTGTGT-CG--CATCGATGC--CAAGCGCTTAAAGCTGTGCGC--GTCTC-CA-T
Clon_TPA1_22 GAGCACTGTGT-CA--CATCGATGCACCAGGCGCTTAAAGCTGTGCGC--GTCTC-CA-T
Clon_TPA1_29 GAGCACTGTGT-CA--CATCGATGCACCAGGCGCTTAAAGCTGTGCGC--GTCTC-CA-T
Clon_TPA1_28 GAGCACTGTGT-CA--CATCGATGCACCAAGCGCTTAAAGCTGTGCGC--GTCTC-CA-T
Clon_TPA1_26 GAGCACTGTGT-CA--CATCGATGCACCAAGCGCTTAAAGCTGTGCGC--GTCTC-CA-T
Clon_TPA1_21 GAGCACTGTGT-CA--CATCGATGC--CAAGCGCTTAAAGCTGTGCGC--GTCTC-CA-T
Clon_TPA1_36 GAGCACTGTGT-CG--CATCGATGCACCAAGCGCTTAAAGCTGTGCGC--GTCTC-CAAT
Clon_TPA1_27 GAGCACTGTGT-CA--CATCGATGCACCAAGCGCTTAAAGCTGTGCGC--GTCTC-CA-T
Clon_TPA1_23 GAGCACTGTGT-CA--CATCGATGCACCAAGCGCTTAAAGCTGTGCGC--GTCTC-CA-T
Clon_TPA1_1 GAGCACTGTGT-CG--CATCGATGC--CAAGCGCTTAAAGCTGTGCGC--GTCTC-CA-T
Clon_TF22 GAGCACTGTGT-CA--CATCGCTGC--TGCGCA-TCAAAACTGTGCAAATTTCTCACTAT
Clon_TF24 GAGCACTGTGT-CA--CATCGCTGC--TGCGCA-TCAAAACTGTGCAAATTTCTCACTAT
Clon_TF3 GAGCACTGTGT-CA--CATCGTTGC--TGCGCA-TCAAAACTGTGCAAATTTCTCACTAT
Clon_TF2 GAGCACTGTGT-CA--CATCGCTGC--TGCGCA-TCAAAACTGTGCAAATTTCTCACTAT
Clon_TF23 GAGCACTGTGT-CA--CATCGCTGC--TGCGCA-TCAAAACTGTGCAAATTTCTCACTAT
Clon_TF1 GAGCACTGTGT-CA--CATCGCTGC--TGCGCA-TCAAAACTGTGCAAATTTCTCACTAT
Clon_TF4 GAGCACTGTGT-CA--CATCGCTGC--TGCGCA-TCAAAACTGTGCAAATTTCTCACTAT
Clon_TF7 GAGCACTGTGT-CA--CATCGTTGC--TGCGCA-TCAAAACTGTGCAAATTTCTCACTAT
Clon_TF15 GAGCACTGTGT-CA--CATCGCTGC--TGCGCA-TCAAAACTGTGCAAATTTCTCACTAT
Clon_TF14 GAGCACTGTGT-CA--CATCGCTGC--TGCGCA-TCAAAACTGTGCAAATTTCTCACTAT
Clon_DP8 AAACACCTCGA-----------------------------ATGTGTGC--------CTTG
Clon_DP1 AAACACCTCGA-----------------------------ATGTGTGC--------CTTG
Clon_DP7 AAACACCTCGA-----------------------------ATGTGTGC--------CTTG
Clon_DP3 AAACACCTCGA-----------------------------ATGTGTGC--------CTTG
Clon_DP6 AAACACCTCGA-----------------------------ATGTGTGC--------CTTG
Clon_DP9 AAACACCTCGA-----------------------------ATGTGTGC--------CTTG
Clon_DP2 AAACACCTCGA-----------------------------ATGTGTGC--------CTTG
Clon_DP4 AAACACCTCGA-----------------------------ATGTGTGC--------CTTG
Clon_DP10 TAACACCTTGA-----------------------------ATGTGTGC--------CTTG
Clon_DP5 AAACACCTTGA-----------------------------ATGTGTGC--------CTTG
Clon_EM4 AAACGCCCGGA-----------------------------ATGTGTGC--------CTTT
Clon_EM21 AAACGCCCGGA-----------------------------ATGTGTGC--------CTTT
Clon_EM2 AAACGCCCGGA-----------------------------ATGTGTGC--------CTTT
Clon_EM23 AAACGCCCGGA-----------------------------ATGTGTGC--------CTTT
Clon_EM3 AAACGCCCGGA-----------------------------ATGTGTGC--------CTTT
Clon_EM24 AAACGCCCGGA-----------------------------ATGTGTGC--------CTTT
Clon_EM22 AAACGCCCGGA-----------------------------ATGTGTGC--------CTTT
Clon_EM1 AAACGCCCGGA-----------------------------ATGTGTGC--------CTTT
Clon_EM6 AAACGCCCTGA-----------------------------ATGTGTGC--------CTTT
Clon_EM5 AAACGCCCGGA-----------------------------ATGTGTGC--------CTTT
Clon_GD1 GAATATTCCAAACTG--ATCATACAACTGATGA-----CAATATGTGC------TTCTGA
Clon_GD10 GAATATTCCAAACTG--ATCATACAACTGATGA-----CAATATGTGC------TTCTGA
Clon_GD2 GAATATTCCAAACTG--ATCATACAACTGATGA-----CAATATGTGC------TTCTGA
Clon_GD5 GAATATTCCAAACTG--ATCATACAACTGATGA-----CAATATGTGC------TTCTGA
Clon_GD3 GAATATTCCAAACTG--ATCATACAACTGATGA-----CAATATGTGC------TTCTGA
Clon_GD12 GAATATTCCAAACTG--ATCATACAACTGATGA-----CAATATGTGC------TTCTGA
Clon_GD7 GAATATTCCAAACTG--ATCATACAACTGATGA-----CAATATGTGC------TTCTGA
Clon_GD9 GAATATTCCAAACTG--ATCATACAACTGATGA-----CAATATGTGC------TTCTGA
Clon_GD8 GAATATTCCAAACTG--ATCATACAACTGATGA-----CAATATGTGC------TTCTGA
Clon_GD13 GAATATTCCAAACTG--ATCATACAACTGATGA-----CAATATGTGC------TTCTGA
Clon_LD5 GAATATTCCAAACCG--ATCAT--AACTGA--------CAATATGTGC------TTCTGA
Clon_LD13 GAATATTCCAAACCG--ATCAT--AACTGA--------CAATATGTGC------TTCTGA
Clon_LD14 GAATATTCCAAACCG--ATCAT--AACTGA--------CAATATGTGC------TTCTGA
Clon_LD1 GAATATTCCAAACCG--ATCAT--AACTGA--------CAATATGTGC------TTCTGA
Clon_LD11 GAATATTCCAAACCG--ATCAT--AACTGA--------CAATATGTGC------TTCTGA
Clon_LD3 GAATATTCCAAACCG--ATCAT--AACTGA--------CAATATGTGC------TTCTGA
Clon_LD2 GAATATTCCAAACCG--ATCAT--AACTGA--------CAATATGTGC------TTCTGA
Clon_LD12 GAATATTCCAAACCG--ATCAT--AACTGA--------CAATATGTGC------TTCTGA
Clon_LD8 GAATATTCCAAACCG--ATCAT--AACTGA--------CAATATGTGC------TTCTGA
Clon_LD15 GAATATTCCAAACCG--ATCAT--AACTGA--------CAATATGTGC------TTCTGA
*
Clon_DM1 TAT-A---------------------------------TTGC------------------
Clon_DM21 TAT-A---------------------------------TTGC------------------
Clon_DM6 TAT-A---------------------------------TCGC------------------
Clon_DM20 TAT-A---------------------------------TTGC------------------
Clon_DM9 TAT-A---------------------------------TTGC------------------
Clon_DM12 TAT-A---------------------------------TTGC------------------
Clon_DM7 TAT-A---------------------------------TTGC------------------
Clon_DM11 TAT-A---------------------------------TTGC------------------
Clon_DM14 TAT-A---------------------------------TTGC------------------
Clon_DMA TAT-A---------------------------------TTGC------------------
Clon_DF1 TATGA---------------------------------TTAC------------------
Clon_DF6 TATGA---------------------------------TTAC------------------
Clon_DF4 TATGA---------------------------------TAAC------------------
Clon_DF26 TATGA---------------------------------TAAC------------------
Clon_DF4_50 TATGA---------------------------------TTAC------------------
Clon_DF19 TATGA---------------------------------TTAC------------------
Clon_DF3 TATGA---------------------------------TTAC------------------
Clon_DF5 TATGA---------------------------------TTAC------------------
Clon_DF2 TATGA---------------------------------TTAC------------------
Clon_DF7 TATGA---------------------------------TTAC------------------
Clon_AS15 TACATTT--CAGTCTCGAATGGTTAATGCAACA----TTTAA-TGCTTGTACC-TTTACT
Clon_AS14 TACATTT--CAGTCTCGAATGGTTAATGCAACA----TTTAA-TGCTTGTACC-TTTACT
Clon_AS20 TACATTT--CAGTCTCGAATGGTTAATGCAACA----TTTAA-TGCTTGTACC-TTTACT
Clon_AS13 TACATTT--CAGTCTCGAATGGTTAATGCAACA----TTTAA-TGCTTGTACC-TTTACT
Clon_AS10 TACATTT--CAGTCTCGAATGGTTAATGCAACA----TTTAA-TGCTTGTACC-TTTACT
Clon_AS11 TACATTT--CAGTCTCGAATGGTTAATGCAACA----TTTAA-TGCTTGTACC-TTTACT
Clon_AS2 TACATTT--CAGTCTCGAATGGTTAATGCAACA----TTTAA-TGCTTGTACC-TTTACT
Clon_AS12 TACATTT--CAGTCTCGAATGGTTAATGCAACA----TTTAA-TGCTTGTACC-TTTACT
Clon_AS1 TACATTT--CAGTCTCGAATGGTTAATGCAACA----TTTAA-TGCTTGTACC-TTTACT
Clon_AS16 TACATTT--CAGTCTCGAATGGTTAATGCAACA----TTTAA-TGCTTGTACC-TTTACT
Clon_BT8 --------------------------------A----TTGA-------------------
Clon_BT9 --------------------------------A----TTGA-------------------
Clon_BT16 --------------------------------A----TTGA-------------------
Clon_BT3 --------------------------------A----TTGA-------------------
Clon_BT14 --------------------------------A----TTGA-------------------
Clon_BT17 --------------------------------A----TTGA-------------------
Clon_BT13 --------------------------------A----TTGAG------------------
Clon_BT1 --------------------------------A----TTGA-------------------
Clon_BT10 --------------------------------A----TTGAG------------------
Clon_BT15 --------------------------------A----TTGAG------------------
Clon_TPA1_20 TACAAT---CAGACTCGGATGA-------AGCC----CTCG-----TAACGA--------
Clon_TPA1_22 TACAAT---CAGACTCGGATGA-------AGCC----CTCG-----TAACGA--------
Clon_TPA1_29 TACAAT---CAGACTCGGATGA-------AGCC----CTCG-----TAACGA--------
Clon_TPA1_28 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TPA1_26 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TPA1_21 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TPA1_36 TACAAT---CAGACTCGGATGA-------AGTA----CTCG-----TAACGA--------
Clon_TPA1_27 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TPA1_23 TACAAT---CAGACTCGGATGA-------AGCC----CTCG-----TAACGA--------
Clon_TPA1_1 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TF22 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TF24 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TF3 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TF2 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TF23 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TF1 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TF4 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TF7 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TF15 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_TF14 TACAAT---CAGACTCGGATGA-------AGCA----CTCG-----TAACGA--------
Clon_DP8 CTTAA--------------------------------CCAA-------------------
Clon_DP1 CTTAA--------------------------------CCAA-------------------
Clon_DP7 CATAA--------------------------------CCAA-------------------
Clon_DP3 TATAA--------------------------------CCAA-------------------
Clon_DP6 CATAA--------------------------------CCAA-------------------
Clon_DP9 TATAA--------------------------------CCAGT------------------
Clon_DP2 TATAA--------------------------------CCAAT------------------
Clon_DP4 TATAA--------------------------------CCGGT------------------
Clon_DP10 TATAA--------------------------------CCAA-------------------
Clon_DP5 CATAA--------------------------------CCAA-------------------
Clon_EM4 TGCTA--------------------------------CTT--------------------
Clon_EM21 TGCTA--------------------------------CTT--------------------
Clon_EM2 TGCTA--------------------------------CTT--------------------
Clon_EM23 TGCTA--------------------------------CTT--------------------
Clon_EM3 TGCTA--------------------------------CTT--------------------
Clon_EM24 TGCTA--------------------------------CTT--------------------
Clon_EM22 TGCTA--------------------------------CTT--------------------
Clon_EM1 TGCTA--------------------------------CTT--------------------
Clon_EM6 TGCTA--------------------------------CTT--------------------
Clon_EM5 TGCTA--------------------------------CTT--------------------
Clon_GD1 TACGAT------ACTTTGGTGAAA-------------CATA--------ATTCTCA-ATT
Clon_GD10 TACGAT------ACTTTGGTGAAA-------------CATA--------ATTCTCA-ATT
Clon_GD2 TACGAT------ACTTTGGTGAAA-------------CATA--------GCTAAAACAAT
Clon_GD5 TACGAT------ACTTTGGTGAAA-------------CATA--------GCTAAAACAAT
Clon_GD3 TACGAT------ACTTTGGTGAAA-------------CATA--------GCTAAAACAAT
Clon_GD12 TACGAT------ACTTTGGTGAAA-------------CATA--------GCTAAAACAAT
Clon_GD7 TACGAT------ACTTTGGTGAAA-------------CATA--------GCTAAAACAAT
Clon_GD9 TACGAT------ACTTTGGTGAAA-------------CATA--------GCTAAAACAAT
Clon_GD8 TACGAT------ACTTTGGTGAAA-------------CATA--------ATTCTCA-ATT
Clon_GD13 TACGAT------ACTTTGGTGAAA-------------CATA--------GCTAAAACAAT
Clon_LD5 TACATTT---GAAATTTGGTGGTA-------------TTTGTTCTGTATGTTGTGTTCTT
Clon_LD13 TACATTT---GAAATTTGGTGGTA-------------TTTGTTCTGTATGTTGTGTTCTT
Clon_LD14 TACATTT---GAAATTTGGTGGTA-------------TTTGTTCTGTATGTTGTGTTCTT
Clon_LD1 TACATTT---GAAATTTGGTGGTA-------------TTTGTTCTGTATGTTGTGTTCTT
Clon_LD11 TACATTT---GAAATTTGGTGGTA-------------TTTGTTCTGTATGTTGTGTTCTT
Clon_LD3 TACATTT---GAAATTTGGTGGTA-------------TTTGTTCTGTATGTTGTGTTCTT
Clon_LD2 TACATTT---GAAATTTGGTGGTA-------------TTTGTTCTGTATGTTGTGTTCTT
Clon_LD12 TACATTT---GAAATTTGGTGGTA-------------TTTGTTCTGTATGTTGTGTTCTT
Clon_LD8 TACATTT---GAAATTTGGTGGTA-------------TTTGTTCTGTATGTTGTGTTCTT
Clon_LD15 TACATTT---GAAATTTGGTGGTA-------------TTTGTTCTGTATGTTGTGTTCTT
Clon_DM1 CAACAT---GGATG------------------AGCATTGTAGAATGTATAACTTTAAATT
Clon_DM21 CAACAT---GGATG------------------AGCATTGTAGAATGTATAACTTTAAATT
Clon_DM6 CAACAT---GGATT------------------AGTATTGTAGAATTTGTAACTTTAAATT
Clon_DM20 CAACAT---GGATG------------------AGCATTGTAGAATGTATAACTTTAAATT
Clon_DM9 CAACAT---GGATT------------------AGTATTGCG-----TGTAAATTGCAATT
Clon_DM12 CAACAT---GGATT------------------AGTATTGCG-----TGTAAATTGCAATT
Clon_DM7 CAACAT---GGATT------------------AGTATTGCG-----TGTAAATTGCAATT
Clon_DM11 CAACAT---GGATG------------------AGCATTGTAGAATGTATAACTTTAAATT
Clon_DM14 CAACAT---GGATT------------------AGCATTGTAGAATGTATAACTTTAAATT
Clon_DMA CAACAT---GGATT------------------AGTATTGCG-----TGTAAATTGCAATT
Clon_DF1 CAACAT---GGATT------------------AACATT----------CAAATTTCAATT
Clon_DF6 CAACAT---GGATT------------------AACATT----------CAAATTTCAATT
Clon_DF4 CAACAT---GGATT------------------AATATT----------CAAATTTCAATT
Clon_DF26 CAACAT---GGATT------------------AATATT----------CAAATTTCAATT
Clon_DF4_50 CAACAT---GGATT------------------AACATT----------CAAATTTCAATT
Clon_DF19 CAACAT---GGATT------------------AACATT----------CAAATTTCAATT
Clon_DF3 CAACAT---GGATT------------------AATATT----------CAAATTTCAATT
Clon_DF5 CAACAT---GGATT------------------AATATT----------CAAATTTCAATT
Clon_DF2 CAACAT---GGATT------------------AACATT----------CAAATTTCAATT
Clon_DF7 CAACAT---GGATT------------------AATATT----------CAAATTTCAATT
Clon_AS15 TGAGAA---GTGTCA-CAATAGTTTAC-CCTTACTAGTAGAGA---CTTGACCGTAAAAC
Clon_AS14 TGAGAA---GTGTCA-CAATAGTTTAC-CCTTACTAGTAGAGA---CTTGACCGTAAAAC
Clon_AS20 TGAGAA---GTGTCA-CAATAGTTTAC-CCTTACTAGTAGAGA---CTTGACCGTAAAAC
Clon_AS13 TGAGAA---GTGTCA-CAATAGTTTAC-CCTTACTAGTAGAGA---CTTGACCGTAAAAC
Clon_AS10 TGAGAA---GTGTCA-CAATAGTTTAC-CCTTACTAGTAGAGA---CTTGACCGTAAAAC
Clon_AS11 TGAGAA---GTGTCA-CAATAGTTTAC-CCTTACTAGTAGAGA---CTTGACCGTAAAAC
Clon_AS2 TGAGAA---GTGTCA-CAATAGTTTAC-CCTTACTAGTAGAGA---CTTGACCGTAAAAC
Clon_AS12 TGAGAA---GTGTCA-CAATAGTTTAC-CCTTACTAGTAGAGA---CTTGACCGTAAAAC
Clon_AS1 TGAGAA---GTGTCA-CAATAGTTTAC-CCTTACTAGTAGAGA---CTTGACCGTAAAAC
Clon_AS16 TGAGAA---GTGTCA-CAATAGTTTAC-CCTTACTAGTAGAGA---CTTGACCGTAAAAC
Clon_BT8 -AAAAA---AC-TC--CAATA-----------ACAA--------------ACAACAAAA-
Clon_BT9 -AAAAA---AC-TC--CAATA-----------ACAA--------------ACAACAAAA-
Clon_BT16 -AAAAA---AC-TC--CAATA-----------ACAA--------------ACAACAAAA-
Clon_BT3 -AAAAA---AC-TC--CAATA-----------ACAA--------------ACAACAAAA-
Clon_BT14 -AAAAA---AC-TC--CAATA-----------ACAA--------------ACAACAAAA-
Clon_BT17 -AAAAA---AC-TC--CAATA-----------ACAA--------------ACAACAAAA-
Clon_BT13 -AAAAA---AC-TC--CAATA-----------ACAA--------------ACAACAAAA-
Clon_BT1 -AAAAA---AC-TC--CAATA-----------ACAA--------------ACAACAAAA-
Clon_BT10 -AAAAA---AC-TC--CAATA-----------ACAA--------------ACAACAAAA-
Clon_BT15 -AAAAA----C-TC--CAATA-----------ACAA--------------ACAACAAAA-
Clon_TPA1_20 TGAACT---GTGTT--TA------CA----CTAAAACTTTG------CATACTGCACATT
Clon_TPA1_22 TGAACT---GTGTT--TA------CA----CTAAAACTTTG------CATACTGCACATT
Clon_TPA1_29 TGAACT---GTGTT--TA------CA----CTAAAACTTTG------CATACTGCACATT
Clon_TPA1_28 TGAACT---GTGTT--TA------CA----CTAAAACTTTG------CATACTGCACATT
Clon_TPA1_26 TGAACT---GTGTT--TA------CA----CTAAAACTTTG------CATACTGCACATT
Clon_TPA1_21 TGAACT---GTGTT--TA------CA----CTAAAACTTTG------CATACTGCGCAC-
Clon_TPA1_36 TGAACT---GTGTT--TA------CA----CTAAAACTTTG------CATACTGCGCAC-
Clon_TPA1_27 TGAACT---GTGTT--TA------CA----CTAAAACTTTG------CATACTGCACATT
Clon_TPA1_23 TGAACT---GTGTT--TA------CA----CTAAAACTTTG------CATACTGCACATT
Clon_TPA1_1 TGAACT---GTGTT--TA------CA----CTAAAACTTTG------CATACTGCACATT
Clon_TF22 TGAATT---GTGTT--TA------CAC--GCTAAAACTTTG------CATACT----TTG
Clon_TF24 TGAATT---GTGTT--TA------CAC--GCTAAAACTTTG------CATACT----TTG
Clon_TF3 TGAATT---GTGTT--TA------CAC--GCTAAAACTTTG------CATACT----TTG
Clon_TF2 TGAATT---GTGTT--TA------CA----CTAAAACTTTG------CATACT----TTG
Clon_TF23 TGAATT---GTGTT--TA------CG----CTAAAACTTTG------CATACTAAAACTG
Clon_TF1 TGAATT---GTGTT--TA------CA----CTAAAACTTTG------CATACT----TTG
Clon_TF4 TGAATT---GTGTT--TA------CA----CTAAAACTTTG------CATACT----TTG
Clon_TF7 TGAATT---GTGTT--TA------CA----CTAAAACTTTG------CATACT----TTG
Clon_TF15 TGAATT---GTGTT--TA------CA----CTAAAACTTTG------CATACT----TTG
Clon_TF14 TGAATT---GTGTT--TA------CG----CTAAAACTTTG------CATACTAAAACTG
Clon_DP8 --AATA---TAGTCG-CAA-------------ATCATTGTC-------CAAAACAAAAC-
Clon_DP1 --AATA---TAGTCG-CAA-------------ATCATTGTC-------CAAAACAAAAC-
Clon_DP7 --AATA---TAGTTG-CAA-------------ATCATTGTA-------CAAAATAAAAC-
Clon_DP3 --AGTA---TAGTCG-CAA-------------ATCATTGTC-------CAA--TAAAA--
Clon_DP6 --AGTA---TAGTCG-CAA-------------ATCATTGTA-------CAAATCAAAAC-
Clon_DP9 -CAAAA---TGGTTGACAA-------------ATAAT-GTA-------CCA-----ATC-
Clon_DP2 -CAAAA---TGGTTGACAA-------------ATCAATGT--------CCAAT--AAAA-
Clon_DP4 -CAAAA---TGGTTGACAA-------------ATCAATGTA-------CCAAT-----C-
Clon_DP10 --AGTA---TAGTCG-CAA-------------ATCATTGT--------CCAAT--AAAA-
Clon_DP5 --AGTA---TAGTCG-CAA-------------ATCATTGTA-------CAAATCAAAAC-
Clon_EM4 ----TC---AAGTCAACAG-------------ATCATTGTT-------CCAAAACAAAT-
Clon_EM21 ----TC---AAGTCAACAG-------------ATCATTGTT-------CCAAAACAAAT-
Clon_EM2 ----TC---AAGTCAACAG-------------ATCATTGTT-------CCAAAACAAAT-
Clon_EM23 ----TC---AAGTCAACAG-------------ATCATTGTT-------CCAAAACAAAT-
Clon_EM3 ----TC---AAGTCAACAG-------------ATCATTGTT-------GCAAAACAAAT-
Clon_EM24 ----TC---AAGTCAACAG-------------ATCATTGTT-------GCAAAACAAAT-
Clon_EM22 ----TC---AAGTCAACAG-------------ATCATTGTT-------CCAAAACAAAT-
Clon_EM1 ----TC---AAGTCAACAG-------------ATCATTGTT-------CCAAAACAAAT-
Clon_EM6 ----TC---AAGTCAACAG-------------ATCATTGTT-------CCAAAA-AAAT-
Clon_EM5 ----TC---AAGTCAACAG-------------ATCATTGTT-------CCAAAA-AAAT-
Clon_GD1 CCAAGT---GTGT-ATGGG-----------CT--AATTTTT------TAG--CGCATATG
Clon_GD10 CCAAGT---GTGT-ATGGG-----------CT--AATTTTT------TAG--CGCATATG
Clon_GD2 CCAAGT---GTGT-ATGGG-----------CT--AATTTTT------TAG--CGCATATG
Clon_GD5 CCAAGT---GTGT-ATGGG-----------CT--AATTTTT------TAG--CGCATATG
Clon_GD3 CCAAGT---GTGT-ATGGG-----------CT--AATTTTT------TAG--CGCATATG
Clon_GD12 CCAAGT---GTGT-ATGGG-----------CT--AATTTTT------TAG--CGCATATG
Clon_GD7 CCAAGT---GTGT-ATGGG-----------CT--AATTTTT------TAG--CGCATATG
Clon_GD9 CCAAGT---GTGT-ATGGG-----------CT--AATTTTT------TAG--CGCATATG
Clon_GD8 CCAAGT---GTGT-ATGGG-----------CT--AATTTTT------TAG--CGCATATG
Clon_GD13 CCAAGT---GTGT-ATGGG-----------CT--AATTTTT------TAG--CGCATATG
Clon_LD5 TCATTT--TGTGTGATTGA-----------TTGTAATTATT------GTGATTACAATTG
Clon_LD13 TCATTT--TGTGTGATTGA-----------TTGTAATTATT------GTGATTACAATTG
Clon_LD14 TCATTT--TGTGTGATTGA-----------TTGTAATTATT------GTGATTACAATTG
Clon_LD1 TCATTT--TGTGTGATTGA-----------TTGTAATTATT------GTGATTACAATTG
Clon_LD11 TCATTT--TGTGTGATTGA-----------TTGTAATTATT------GTGATTACAATTG
Clon_LD3 TCATTT--TGTGTGATTGA-----------TTGTAATTATT------GTGATTACAATTG
Clon_LD2 TCATTTGTTGTGTAATTGA-----------TTGTAATTATT------GTGATTACAATTG
Clon_LD12 TCATCT--TGTGTGATTGA-----------TTGTAATTATT------GTGATTACAATTG
Clon_LD8 TCATTTGTTGTGTAATTGA-----------TTGTAATTTTT-----------TACAATTG
Clon_LD15 TCATTTGTTGTGTAATTGA-----------TTGTAATTTTT-----------TACAATTG
* *
Clon_DM1 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DM21 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DM6 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DM20 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DM9 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DM12 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DM7 --------------------GTCTATATTCGACCTCAGATCGAGCGAGACTA---
Clon_DM11 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DM14 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DMA --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DF1 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DF6 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DF4 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DF26 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DF4_50 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DF19 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DF3 --------------------GTCTATATTCGATCTCAGATCAAGCGAGACTA---
Clon_DF5 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DF2 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_DF7 --------------------GTCTATATTCGACCTCAGATCAAGCGAGACTA---
Clon_AS15 AG-TCACTGATCTTCCGGT-GTTAACCTTCGACCTCAGGTCAAGCGAGATTA---
Clon_AS14 AG-TCACTGATCTTCTGGT-GTTAACCTTCGACCTCAGGTCAAGCGAGATTA---
Clon_AS20 AG-TCACTGATCTTCTGGT-GTTAACCTTCGACCTCAGGTCAAGCGAGATTA---
Clon_AS13 AG-TCACTGATCTTCTGGT-GTTAACCTTCGACCTCAGGTCAAGCGAGATTA---
Clon_AS10 AG-TCACTGATCTTCTGGT-GTTAACCTTCGACCTCAGGTCAAGCGAGATTA---
Clon_AS11 AG-TCACTGATCTTCTGGT-GTTAACCTTCGACCTCAGGTCAAGCGAGATTA---
Clon_AS2 AG-TCACTGATCTTCTGGT-GTTAACCTTCGACCTCAGGTCAAGCGAGATTA---
Clon_AS12 AG-TCACTGATCTTCTGGT-GTTAACCTTCGACCTCAGGTCAAGCGAGATTA---
Clon_AS1 AG-TCACTGATCTTCTGGT-GTTAACCTTCGACCTCAGGTCAAGCGAGATTA---
Clon_AS16 AG-TCACTGATCTTCTGGT-GTTAACCTTCGACCTCAGGTCAAGCGAGATTA---
Clon_BT8 ---TCA---AAGTTTT----GTCAAA-TTCGACCTCAGATCAAGCGAGATTA---
Clon_BT9 ---TCA---AAGTTTT----GTCAAA-TTCGACCTCAGATCAAGCGAGATTA---
Clon_BT16 ---TCA---AAATTTT----GTCAAA-TTCGACCTCAGATCAAGCGAGATTA---
Clon_BT3 ---TCA---AATTTTT----GTCAAA-TTCGACCTCAGATCAAGCGAGATTA---
Clon_BT14 ---TCA---AATTTTT----GTCAAA-TTCGACCTCAGATCAAGCGAGATTA---
Clon_BT17 ---TCA---AAGTTTT----GTCAAA-TTCGACCTCAGATCAAGCGAGATTA---
Clon_BT13 ---TCA---AAGTTTT----GTCAAA-TTCGACCTCAGATCAAGCGAGATTA---
Clon_BT1 ---TCA---AAGTTTT----GTCAAA-TTCGACCTCAGATCAAGCGAGATTA---
Clon_BT10 ---TCA------TTTT----GTCAAA-TTCGACCTCAGATCAAGCGAGATTA---
Clon_BT15 ---TCA---AAATTTT----GTCAAA-TTCGACCTCAGATCAAGCGAGATTA---
Clon_TPA1_20 GATTTGT----GCAGTTGTTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TPA1_22 GATTTGT----GCAGTTGTTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TPA1_29 GATTTGT----GCAGTTGTTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TPA1_28 GATTTGT----GCAGTTGTTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TPA1_26 GATTTGT----GCAGTTGTTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TPA1_21 -ATTTGT----GCAGTTGTTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TPA1_36 -ATTTGT----GCAGTTGTTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TPA1_27 GATTTGT----GCAGTTGTTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TPA1_23 GATTTGT----GCAGTTGTTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TPA1_1 GATTTGT----GCAGTTGTTGTTAACTITCGACCTCAGATCAAGCGAGATTA---
Clon_TF22 TG--CA-----GATGT GTTGTTAACTITCGACCTCAGATCAAGCGAGATTA---
Clon_TF24 TG--CA-----GATGT-GTTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TF3 TGTGCA-----GTTGT--TTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TF2 TG--CA-----GATGT GTTGTTAACTITCGACCTCAGATCAAGCGAGATTA---
Clon_TF23 TG--CA-----GTTGT--TTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_TF1 TG--CA-----GATGT-GTTGTTAACTITCGACCTCAGATCAAGCGAGATTA---
Clon_TF4 TG--CA-----GATGT-GTTGTTAACTITCGACCTCAGGTCAAGCGAGATTA---
Clon_TF7 TG--CA-----GATGT-GTTGTTAACTITCGACCTCAGATCAAGCGAGATTA---
Clon_TF15 TG--CA-----GATGT-GTTGTTAACTITCGACCTCAGATCAAGCGAGATTA---
Clon_TF14 TG--CA-----GTTGT--TTGTTAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_DP8 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGAATA---
Clon_DP1 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGAATA---
Clon_DP7 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGAATA---
Clon_DP3 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGAATA---
Clon_DP6 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGAATA---
Clon_DP9 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGAATA---
Clon_DP2 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGAATA---
Clon_DP4 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGAATA---
Clon_DP10 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGAATA---
Clon_DP5 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGAATA---
Clon EM4 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGACTA---
Clon EM21 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGACTA---
Clon_EM2 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGACTA---
Clon EM23 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGACTA---
Clon EM3 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGACTA---
Clon EM24 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGACTA---
Clon_EM22 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGACTA---
Clon EM1 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGACTA---
Clon EM6 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGACTA---
Clon EM5 --------------------GTCTAATTTCGACCTCAGATCAAGCGAGACTA---
Clon GD1 TTTTT------------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_GD10 TTTTT------------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_GD2 TTTTT------------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_GD5 TTTTT------------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_GD3 TTTTT------------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_GD12 TTTTT------------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_GD7 TTTTT------------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_GD9 TTTTT------------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_GD8 TTTTT------------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_GD13 TTTTT------------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_LD5 ATATTG-----------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_LD13 ATATTG-----------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_LD14 ATATTG-----------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_LD1 ATATTG-----------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_LD11 ATATTG-----------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_LD3 ATATTG-----------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_LD2 ATATTG-----------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_LD12 ATATTG-----------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_LD8 ATATTG-----------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
Clon_LD15 ATATTG-----------TTTGTAAACTTTCGACCTCAGATCAAGCGAGATTA---
** * ***** ***** ** ******* **
Example 1 DNA extraction from single individuals: Individuals are carefully isolated under a stereoscopic microscope by the aid of entomological needles. If they came from a lot pools preserved in ethanol, wash once in a clean ethanol 70% solution. Each individual is deposited on a 1.5 ml micro-tube place on ice.
This protocol has been also successfully applied to extract DNA from 10-50 individuals in 70% ethanol. In this case, ethanol is removed after centrifugation (14000 g, 5 seconds).
1. Add 100 μl of Extraction Buffer (Tris-HCl 10 mM pH 8.0, EDTA 25 mM pH 8.0 y NaCl 100 mM) and homogenise by the aid of a Pellet pestle adapted to the micro-tube (e.g. Pellet pestle, Sigma). A good homogenisation is crucial.
2. Add 10 μl of SDS al 10% (1% final) and mix gently.
3. Add 10 μl of proteinase K (stock: 10 mg/ml) (Final 1 mg/ml), mix gently and incubate 37° C., 2 hours.
4. Add 20 μl of NaCl 5M (final≈0.5 M NaCl) and mix gently.
5. Add 15 μl of cetyltrimethylammonium bromide (CTAB) (Stock: CTAB 10% in NaCl 0.5M, 62° C.) and incubate for 10 minutes at 62° C. Mix gently several times during incubation.
6. Add 0.5 volumes of equilibrated phenol and 0.5 volumes of chloroform: isoamyl alcohol (24:1). Mix gently for two minutes and centrifuge at 11000 g for 3 minutes.
7. Carefully remove approximately 110 μl of the aqueous phase and transfer to a clean micro-tube. Add 0.5 volumes of distilled water (˜55 μl), add 1 volume of isopropanol (˜170 μl), mix gently, wait two minutes and centrifuge at 18000 g for 15 minutes.
8. Remove isopropanol immediately after centrifugation by inverting the tube. Add 700 μl of 70% ethanol to wash the pellet (generally, it cannot be seen) and centrifuge at 18000 g for 15 minutes.
9. Remove ethanol immediately after centrifugation by inverting the tube and place the tube containing the pellet to let it dry on the bench or in a laminar flow cabinet (drying should be completed before 1 hour) (do not let dry more than necessary).
10. Add 20 μl of TE 0.1× (Stock TE 1×: Tris-HCl 10 mM pH 8.0 y EDTA 10 mM pH 8.0). 11. Quantify DNA extraction from mite cultures: The same protocol, but adding 4× volumes in points 1, 2, 3, 4, 5, 7, 8 and adding RNAase (0.1 μg/μl) in point 10, has also been applied for DNA extraction from 20 mg of frozen mite cultures.
DNA extraction from environmental samples or purified mite fractions: Use DNeasy Blood and Tissue Kit (Qiagen) from purified fractions (bodies or faeces, 20 mg) and environmental samples (50 mg) and follow manufacturer instructions for purification of total DNA.
Example 2 One-Step Multiplex-PCR Using One or More First (Forward) Primers and a Single Second (Reverse) Primer Hybridising to 5.8S Tested for species identification in cultures of all species, and in purified fractions of Dermatophagoides pteronyssinus and D. farinae.
Identifies the following species DNA: Tyrophagus fanetzhangorum, Lepidoglyphus destructor, Glycyphagus domesticus, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Blomia tropicalis, Euroglyphus maynei, Dermatophagoides microceras, Acarus siro, Dermatophagoides farinae.
1. DNA extraction. See Example 1
3. Multiplex-PCR. Primers: The reaction may be performed
a) using the combination of the set of ten forward (first) primers (see Table 1) with the reverse (second) primer RAst5.8S (5′-TGCGTTCGAAWGTCGAGT-3′), W=A or T
b) using any combination of two or more primers, the reverse (second) primer being one of them.
PCR reaction: a final volume of 25 μL contains
50-150 ng of DNA template
1×PCR Buffer II
200 μM dNTP mix
0.6 μM forward primers mix [0.06 μM each in this case where all ten are used]1 (Table 1)
0.6 μM reverse primer (RAst5.8S)
1.5 mM MgCl2
0.6 mg/mL purified BSA (New England Biolabs, ref. B9001S)
1 U de AmpliTaq Gold DNA Polymerase (Applied Biosystems)
PCR Cycle: PCR Cycle: One hold 10 min 95° C., 40 cycles [30s 95° C., 30s 58° C., 2 min 72° C.], 1 hold 10 min 72° C. PCR products are visualised in agarose gel at 3% [NuSieve low melting agarose (Lonza): D-2 Agarose (Pronadisa), 1:1 proportion]. Results obtained are shown in FIG. 1.
TABLE 1
Forward primers for the Multiplex-PCR. The
approximate size of the amplicon produced by each
forward primer in combination with the reverse
primer RAst5.8S is indicated in the name of the
forward primers.
Primer Sequences (Species)
F1Tf_824 GACAGAAGCTGAAAGCCGT
(Tyrophagus fanetzhangorum)
F1Ld_608 GATGTTCGAATCAATTGCTAGTG
(Lepidoglyphus destructor)
F1Gd_567 GCATACCGTGTTGAAGCAGG
(Glycyphagus domesticus)
F1Dp_501 GATCGACTGGCAATTGTTGAC
(Dermatophagoides pteronyssinus)
F1Tp_474 CGCCATTTGACACAGTACC
(Tyrophagus putrescentiae)
F1Bt_419 TGTGTGTGGGGGATTTTGC (Blomia tropicalis)
F1Em_384 GAGCCTGACAATTATCAATGTGC
(Euroglyphus maynei)
F1Dm_304 CGGGATGAACGTGTGGATG
(Dermatophagoides micro ceras)
F1As_234 GTCGGTTACGGTCAAACG (Acarus siro)
F1Df_159 GAAACAATTGAATTGTGATTCTGC
(Dermatophagoides farinae)
Example 3 Two-Step Multiplex-PCR Required for the analysis of environmental samples, samples showing a low efficiency in the PCR after performing Example 2, or analysis of contaminations in cultures. Identifies the following species DNA: Tyrophagus fanetzhangorum, Lepidoglyphus destructor, Glycyphagus domesticus, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Blomia tropicalis, Euroglyphus maynei, Dermatophagoides micro ceras, Acarus siro, Dermatophagoides farinae.
1. DNA extraction. See Example 1
2. ITS1-ITS2 amplification.
Primers:
FNav
(5′-AGAGGAAGTAAAAGTCGTAACAAG-3′)
and
RNav2
(5-ATATGCTTAAATTCAGCGGG-3′)
PCR reaction: a final volume of 25 μL contains
50-150 ng of DNA template
1×PCR Buffer II
200 μM dNTP mix
0.4 μM each primer (FNav and RNav2)
1.5 mM MgCl2
1 U de AmpliTaq Gold DNA Polymerase (Applied Biosystems)
PCR Cycle: One hold 10 min 95° C., 40 cycles [30s 95° C., 30s 58° C., 2 min 72° C.], 1 hold 10 min 72° C. PCR products may not be visualised after gel electrophoresis.
3. Multiplex-PCR amplification.
Primers: The reaction may be performed
-
- a) using the combination of the set of 10 forward primers (see Table 2) with the reverse primer RAst5.8S (5′-TGCGTTCGAAWGTCGAGT-3′), W=T or A
- b) using any combination of two or more primers, the reverse primer being one of them.
PCR reaction: a final volume of 25 μL contains
5 μl of the PCR products obtained in step 2, dilution 1/500 in MQ water
1×PCR Buffer II
200 μM dNTP mix
0.6 μM forward primers mix [0.06 μM each] (Table 2)
0.6 μM reverse primer (RAst5.8S)
1.5 mM MgCl2
0.6 mg/mL purified BSA (New England Biolabs, ref. B9001S)
1 U de AmpliTaq Gold DNA Polymerase (Applied Biosystems)
PCR Cycle: One hold 10 min 95° C., 35 cycles [30s 95° C., 60s 62° C.], 1 hold 10 min 72° C. PCR products are visualised in agarose gel at 3% [NuSieve low melting agarose (Lonza): D-2 Agarose (Pronadisa), 1:1 proportion]., Results obtained are shown in FIG. 2.
TABLE 2
Forward primers for the Multiplex-PCR. The size
of the amplicon produced by each forward primer
in combination with the reverse primer RAst5.8S
is indicated in the name ofthe forward primers.
Primer Sequence (species in parenthesis)
F1Tf_824 GACAGAAGCTGAAAGCCGT
(Tyrophagus fanetzhangorum)
F1Ld_608 GATGTTCGAATCAATTGCTAGTG
(Lepidoglyphus destructor)
F1Gd_567 GCATACCGTGTTGAAGCAGG
(Glycyphagus domesticus)
F1Dp_501 GATCGACTGGCAATTGTTGAC
(Dermatophagoides pteronyssinus)
F1Tp_474 CGCCATTTGACACAGTACC
(Tyrophagus putrescentiae)
F1Bt_419 TGTGTGTGGGGGATTTTGC
(Blomia tropicalis)
F1Em_384 GAGCCTGACAATTATCAATGTGC
(Euroglyphus maynei)
F1Dm_304 CGGGATGAACGTGTGGATG
(Dermatophagoides micro ceras)
F1As_234 GTCGGTTACGGTCAAACG (Acarus siro)
F1Df_159 GAAACAATTGAATTGTGATTCTGC
(Dermatophagoides farinae)
Example 4 Marker Adapted for Identification of Allergy-Causing Mites (Ma Marker) 1. PCR amplification of marker bands.
ITS1 marker bands for each species are obtained by PCR amplification following Example 3, and increasing the total volume of the PCRs to 100 μL (increase the template and the units of polymerase proportionally).
Perform a gel in order to verify the correct size of the PCR products.
2. Marker bands mix
a. Purify PCR products using a standard commercial kit.
b. Quantify DNA by a standard method to obtain the concentration (ng/μL). A minimum concentration of 100 ng/μL should be obtained
c. In base to the concentration, calculate the volume (μL) of each PCR product that would contain 5 μg of DNA.
d. Multiply the volumes calculated in step “c” by their corresponding correction factors shown Table 3 (volumes are corrected in base to the size of the amplicons), and introduce the resulting volumes in clean micro-tubes (one micro-tube for each PCR product).
e. Add MQ water to each micro-tube till a total volume of 50 μL and mix by vortex.
f. To verify that all calculations are correct, run an agarose gel, charging in different lanes 1 ul of each PCR product prepared in step “e”. Net bands of similar intensity should be seen for all PCR products.
g. If all bands show the same intensity, continue in step h.
h. If the intensity of some bands is low, add 1-10 μL of the purified PCR products to the corresponding micro-tubes in order increase the DNA contents. Continue again in “step f”.
i. Mix the content of the ten micro-tubes prepared in step “e” in a single vial, adding 50 μL of a standard 10× blue sample buffer.
j. To use the marker, charge 5-10 μL in agarose gels.
TABLE 3
Approximate size of PCR products obtained by the amplification
of DNA from different species by Two-step Multiplex-PCR
(Example 3) and correction factors to prepare the Ma Marker.
Species Approx. Size of PCR product (bp) Correction factor in bold
Tyrophagus fanetzhangorum (Tf) 824; 0.19
Lepidoglyphus destructor (Ld) 608; 0.26
Glycyphagus domesticus (Gd) 567; 0.28
Dermatophagoides pteronyssinus (Dp) 501; 0.32
Tyrophagus putrescentiae (Tp) 474; 0.34
Blomia tropicalis (Bt) 419; 0.38
Euroglyphus maynei (Em) 384; 0.41
Dermatophagoides microceras (Dm) 304; 0.52
Acarus siro (As) 234; 0.68
Dermatophagoides farinae (Df) 159; 1.00
Example 5 One-Step Multiplex-PCR Using One or More First (Reverse) Primers and a Single Second (Forward) Primer Hybridising to 18S Tested for species identification of D. pteronyssinus, D. farinae and/or B. tropicalis in cultures of the ten species: Tyrophagus fanetzhangorum, Lepidoglyphus destructor, Glycyphagus domesticus, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Blomia tropicalis, Euroglyphus maynei, Dermatophagoides micro ceras, Acarus siro, Dermatophagoides farinae.
1. DNA extraction. See Example 1
3. Multiplex-PCR. Primers: The reaction may be performed
a) using the combination of the set of three reverse (first) primers (see Table 4 below) with the forward (second) primer FRibNav (5′-AGAGGAAGTAAAAGTCGTAACAAG-3′)
b) using any combination of two or more primers, the forward (second) primer being one of them.
PCR reaction: a final volume of 25 μL contains
50-150 ng of DNA template
1×PCR Buffer II
200 μM dNTP mix
0.6 μM reverse primers mix [0.2 μM of each in this case where all three are used] (Table 4)
0.6 μM forward primer (FRibNav)
1.5 mM MgCl2
0.6 mg/mL purified BSA (New England Biolabs, ref. B9001S)
1.5 U AmpliTaq Gold DNA Polymerase (Applied Biosystems)
PCR Cycle: One hold 10 min 95° C., 40 cycles [30s 95° C., 30s 58° C., 2 min 72° C.], 1 hold 7 min 72° C. PCR products are visualised in agarose gel at 3% [NuSieve low melting agarose (Lonza): D-2 Agarose (Pronadisa), 1:1 proportion]. Results obtained are shown in FIG. 6.
TABLE 4
Reverse (first) primers for the Multiplex-PCR. The
approximate size of the amplicon produced by each
reverse primer in combination with the forward
primer FRibNav is indicated in the name of the
reverse primers.
Primer Sequences (Species)
R1Dp_181 GCTTTCAATAACCTCATCAGTGTC
(Dermatophagoides pteronyssinus)
R1Bt_347 CCATCACTAAAGGACAGAACCGC
(Blomia tropicalis)
R1Df_419 CTCCAGCAATCGAATTATGCTC
(Dermatophagoides farinae)
REFERENCES
- Cruickshank R H (2002) “Molecular markers for the phylogenetics of mites and ticks”. System Appl Acarol 7:3-14.
- Lava Kumar, P., Fenton, B., Jones, A. T. (1999) Identi®cation of Cecidophyopsis mites (Acari: Eriophyidae) based on variable simple sequence repeats of ribosomal DNA internal transcribed spacer-1 sequences via multiplex PCR. Insect Molecular Biology. 1999; 8(3); 347-357
- Navajas, M., Lagnel, J., Fauvel, G. & de Moraes, G. (1999) Sequence variation of ribosomal internal transcribed spacers (ITS) in commercially important Phytoseiidae mite. Experimental and Applied Acarology, 23, 851-859.
- Noge K, Mori N, Tanaka C, Nishida R, Tsuda M, Kuwahara Y. Identification of astigmatid mites using the second internal transcribed spacer (ITS2) region and its application for phylogenetic study. Exp Appl Acarol. 2005; 35:29-46.
- Spieksma F T M, “Identification of house-dust mites”, Aerobiologia 1990; 187-192.
- Suarez-Martinez E B, Montealegre F, Sierra-Montes J M. Molecular identification of pathogenic house dust mites using 12S rRNA sequences. Electrophoresis. 2005; 26:2927-34.
- Thet-Em T, Tungtrongchitr A, Tiewcharoen S, Malainual N., “Multiplex PCR for identifying common dust mites species (D. pteronyssinus, D. farinae and B. tropicalis)”, Asian Pac J Allergy Immunol 2012; 30:224-30.
- Wong S F, Chong A L, Mak J W, Tan J, Ling S J, Ho T M. “Molecular identification of house dust mites and storage mites”. Exp Appl Acarol. 2011; 55:123-33.
