Bacillus thuringiensis Tn5401 proteins
A transposable element, or transposon, isolated from Bacillus thuringiensis (B.t.) and designated as transposon Tn5401. The invention also includes a method of using this transposon in a site-specific recombination system for construction of recombinant B.t. strains that contain insecticidal B.t. toxin protein genes and that are free of DNA not native to B.t.
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Claims
1. An isolated Tn5401 resolvase protein having the amino acid sequence identified by amino acids 1 through 306 of SEQ ID NO:2.
2. An isolated Tn5401 transposase protein having the amino acid sequence identified by amino acids 1 through 1005 of SEQ ID NO:3.
Referenced Cited
U.S. Patent Documents
Foreign Patent Documents
Other references
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Patent History
Patent number: 5843744
Type: Grant
Filed: Jun 24, 1994
Date of Patent: Dec 1, 1998
Assignee: Ecogen Inc. (Langhorne, PA)
Inventor: James A. Baum (Doylestown, PA)
Primary Examiner: Nancy T. Vogel
Law Firm: Panitch Schwarze Jacobs & Nadel, P.C.
Application Number: 8/266,408
Type: Grant
Filed: Jun 24, 1994
Date of Patent: Dec 1, 1998
Assignee: Ecogen Inc. (Langhorne, PA)
Inventor: James A. Baum (Doylestown, PA)
Primary Examiner: Nancy T. Vogel
Law Firm: Panitch Schwarze Jacobs & Nadel, P.C.
Application Number: 8/266,408
Classifications
Current U.S. Class:
Enzyme (e.g., Ligases (6. ), Etc.), Proenzyme; Compositions Thereof; Process For Preparing, Activating, Inhibiting, Separating, Or Purifying Enzymes (435/183);
Acting On Ester Bond (3.1) (435/196);
435/1723
International Classification: C12N 900; C12N 916; C12N 1500;
International Classification: C12N 900; C12N 916; C12N 1500;