Abstract: A method of inducing genetic recombination, including: allowing a protein having DNA double-stranded cleavage activity to act in cells of a eukaryotic organism which is a polyploidy inherently possessed by a eukaryotic organism. In eukaryotic organisms, various genetic recombination generates new genome set composition. This is done to obtain a population of eukaryotic organisms that hold the modified genomic set.

Abstract: A process for harvesting fish eggs is provided, in particular fish eggs from Salmon. The process comprises rearing sexually immature salmon in an aquatic environment in stages during which at least the light exposure and time span is adjusted. The rearing includes at least a winter-summer period that comprises a winter life-cycle stage, within which the broodstock is exposed to light that simulates winter light exposure, and a subsequent summer life-cycle stage, within which the broodstock is exposed to light that simulates summer light exposure, wherein the total Accumulated Thermal Unit (ATU) during the winter-summer period is no more than 5000. The inventions also provides salmon eggs that are produced by the disclosed process.

Abstract: Provided are a method of maintaining the ovaries of marine fish, and a method of obtaining fertilized egg. Provided is a method of maintaining ovaries in which the ovaries are removed from marine fish and the ovaries or fragmented ovaries are cultured in a culture solution. Also, provided is a method of obtaining fertilized eggs of marine fish, the method including allowing ovaries, which have been maintained after removal to ovulate and performing artificial insemination. Further, the present invention relates to a method of maintaining the fertilization capability of ovulated eggs of marine fish by using the culture solution. According to the present invention, fertilized eggs of marine fish can be easily obtained and seed can be efficiently produced.

Abstract: The present invention provides a method for improving the quality of sturgeon meat by using deep seawater with low temperature, clean and rich nutrient characteristics to acclimate the sturgeons for at least 2 weeks. The salinity of the deep seawater is adjusted to 10-20 ppt, water temperature below 20° C. by adding fresh water. Thereby, the taste and flavor of the sturgeon meat can be significantly improved, and the value thereof can also be increased.

Abstract: A recirculating aquaculture system automatically controls ammonia, bacteria, solids, and feed quantity available to a captive species in a closed, water-based habitat. Functionally Independent processing loops operate in parallel on recirculating water streams, to control each of ammonia, bacteria, solids, and feed. The same or similar components, such as an electrolytic cell, may service one or more functional loops.

Abstract: According to an example, artificial ovulation and spawning of freshwater eels may be induced to promote artificial ovulation without employing intraperitoneal injection, by feeding a feminization-inducing material (e.g., 17?-estradiol) for inducing feminization during a predetermined period into a raising bath in which broodfish are raised and, when an ovulation induction period arrives, adding pituitary extracts of catadromous fish as an artificial ovulation-promoting substance to the bath. Raising water of the raising bath may be prepared by selectively setting fresh water, brackish water and seawater conditions, a feminization-inducing material for inducing complete feminization of broodfish of the freshwater eels is fed to the raising bath, including the raising water under a fresh water condition, and pre-treatment for feminization is performed.

Abstract: An otter habitation section, an overland section, an inclined-surface section, a first spawning section, a second spawning section and a roost are all provided. Accordingly, otters and birds can sufficiently obtain preys, and fishes secure a spawning ground suitable for spawning characteristics. Therefore, the otters, the fishes and the birds can inhabit together. Further, since frames are made of wood for providing buoyant force or the frames are made of stainless steel for reinforcing strength and wood for providing buoyant force, a small amount of polyethylene foam is required. Accordingly, it is possible to obtain floating wetland equipment with a simple structure. Furthermore, the wooden frames itself provide a spawning ground of fishes.

Abstract: The invention relates to a method for obtaining a triploid interspecific hybrid of meagre and red drum belonging to the family of the Sciaenidae. The method includes the following two steps: a) crossing a meagre Argyrosomus regius with a red drum Sciaenops ocellatus, and b) carrying out treatment to induce triploidy in the issue obtained.

Abstract: An otter habitation section, an overland section, an inclined-surface section, a first spawning section, a second spawning section and a roost are all provided. Accordingly, otters and birds can sufficiently obtain preys, and fishes secure a spawning ground suitable for spawning characteristics. Therefore, the otters, the fishes and the birds can inhabit together. Further, since frames are made of wood for providing buoyant force or the frames are made of stainless steel for reinforcing strength and wood for providing buoyant force, a small amount of polyethylene foam is required. Accordingly, it is possible to obtain floating wetland equipment with a simple structure. Furthermore, the wooden frames itself provide a spawning ground of fishes.

Abstract: A method for the mass production of fish belonging to the order of Cypriniforms, notably to the family of Cobitidae, from spawners which are raised in closed circuit.

Abstract: An apparatus for testing and researching aquatic species is provided. The apparatus includes a housing rack for housing a tank. The apparatus further includes one or more dividers for dividing the at least one tank into one or more compartments. The apparatus further includes a collection channel and a downspout in each compartment for draining of water from each compartment. The apparatus further includes one or more lids for covering the one or more compartments.

Abstract: It is an object of the invention to provide a method and an apparatus for processing a very large number of fish eggs for gene injection and so on with a superior performance. An egg case has an egg-arranging plate and a cover plate. The fish eggs are dropped into concave portions of the egg-arranging plate, when the cover plate opens the concave portions. The cover plates closes the concave portions after fish eggs are accommodated in the concave portions. The egg case is fixed on a table of an egg-arranging apparatus. water is supplied on the egg-arranging plate in the egg plate. A scanning nozzle sprays water on upper surface of the egg-arranging plate. After closing the concave portions with the cover plate, the egg case is inclined for dropping fish eggs remaining on the cover plate.

Abstract: A method and system for producing large quantities of aquatic animal embryos includes providing a water filled spawning tank adapted for holding the male and female aquatic animals in various configurations. The system can include a spawning platform which includes a porous or perforated element that allows the embryos but not the aquatic animal to pass through and a separator which includes a porous or perforated element that can be used to separate the male aquatic animals from the female aquatic animals during a priming phase. In operation, the spawning platform can be placed in the bottom of the tank in order to provide a bottom collection area where the embryos can be collected and the aquatic animals cannot eat or otherwise harm the embryos. The female aquatic animals can be placed in tank above the spawning platform.

Abstract: A sustainable food production system, known as aquaponics, is housed in at least a portion of an intermodal shipping container. In some embodiments, the food production system can be housed fully in one intermodal shipping container or may span multiple shipping containers that can be stacked vertically, end-to-end or side-to-side. An ecosystem is created in the shipping containers where fish fertilize plants and plants clarify the water for the fish. The containers are designed to be plug-and-play and are expandable. Because they are intermodal (ISO) shipping containers, they can be easily shipped anywhere in the world. In the area of a standard parking space (8 feet by 20 feet), about 1,500 fish per year and hundreds of vegetables monthly can be raised.

Abstract: It is an object of the invention to provide a method for processing a very large number of fish eggs for gene injection and so on, which employs a simple system with high performance and a reasonable construction expense. It is another object to provide the method having an water tank apparatus capable of producing a large number of fish eggs continuously. A large number of water tank groups having an independent drainage passage each is lighted up in turn with a predetermined interval. An egg-collecting case with a net bottom is set in the drainage passage connecting to the water tank group to which the lighting is started. The water tank group consists of a plurality of tank unit accommodating fishes each. Each book-shaped tank unit has a rectangular-shaped upper portion and a cone-shaped lower portion. Rotating water stream is formed in each tank unit.

Abstract: It is an object of the invention to provide a method for processing a very large number of fish eggs for gene injection and so on, which employs a simple system with high performance and a reasonable construction expense. It is another object to provide the method having an water tank apparatus capable of producing a large number of fish eggs continuously. A large number of water tank groups having an independent drainage passage each is lighted up in turn with a predetermined interval. An egg-collecting case with a net bottom is set in the drainage passage connecting to the water tank group to which the lighting is started. The water tank group consists of a plurality of tank unit accommodating fishes each. Each book-shaped tank unit has a rectangular-shaped upper portion and a cone-shaped lower portion. Rotating water stream is formed in each tank unit.

Abstract: The present invention refers to a microfluidic continuous flow devices for culturing biological material each comprising a cultivation chamber being dimensioned to retain a biological material and having an inlet and an outlet to allow flow of a cultivation medium through the cultivation chamber. The present invention also refers to a method using the microfluidic continuous flow device of the present invention and the uses for these devices. In one example a microfluidic continuous flow device of the present invention is connected to a gradient generator.

Abstract: An artificial decapeptide for inducing vitellogenesis in fish, being an analogue of gonadotropin-releasing hormone of chicken is set forth in SEQ ID NO: 2. The artificial decapeptide can be further developed and manufactured into a preparation, which is capable of inducing vitellogenesis in fish. With the implantation of the preparation into body cavity or body wall of bony fish, the induction of vitellogenesis and ovum maturation in bony fish can be successfully achieved.

Abstract: An adjustable apparatus for fish spawning that is suspended below a buoyant float system at a predetermined depth. The apparatus is resistant to vertical motion by surface waves, and may provide additional aeration for egg species that require a high level of dissolved oxygen in order to incubate successfully. The apparatus may be constructed so as to provide maximum or minimum sunlight exposure to spawning bed nests and to accommodate the spawning depth and/or preferred bed slopes of certain fish species.

Abstract: A method of manipulating a spawning cycle of perch across multiple generations that includes identifying a desired out-of-cycle spawning period for perch, selecting a geographic strain of perch having a natural spawning period in proximity to the desired out-of-cycle spawning period, developing a first generation broodstock from the geographic strain, where the broodstock's natural spawning period has been shifted to the desired spawning period using at least one of temperature and photoperiod manipulation, identifying a second desired out-of-cycle spawning period, developing a second generation broodstock from the first generation broodstock, where the second generation broodstock's spawning period has been shifted from the desired out-of-cycle spawning period to the second desired out-of-cycle spawning period using at least one of temperature and photoperiod manipulation.

Abstract: The invention provides for an apparatus (1) for growing aquatic animals comprising at least two securing members (3, 6) adapted to be removably fixed together such that a feed substrate (7) can be reversibly sandwiched between the securing members. The invention also provides for modular assemblies of the apparatus, and further provides methods of culturing aquatic animals and methods of increasing roe content of aquatic animals.

Abstract: The application is directed to an aquatic egg collection research system. The egg collection includes stepped racks that hold a plurality of tanks in a stepped configuration. A stepped fluid conduit assembly and stepped drain respectively supply water to the tanks and collect water flowing out from the tanks. A barrier partitions the tanks enabling eggs to fall through but prevents the adult fish from scavenging the eggs. Nets are placed by a user in strategic areas for trapping eggs from the water flowing out from the tanks. In some embodiments, the tanks are totally opaque, internally lighted, and have an electronic timer for controlling the internal lighting in the tanks. The eggs can be studied with various microscopic slides customized for the research.

Abstract: The invention provides for a process that enables live-bearing fresh water fish to live and breed in a salt water environment such as an aquarium. The process starts with a live birth of fry in an aquarium where the salinity of the water matches the salinity of amniotic fluids. Once the fry stabilize, the salinity can be gradually increased to match the salinity of a standard salt water aquarium. The fry and their offspring mature, live, and breed in salt water with no future intervention.

Abstract: An adjustable apparatus for fish spawning that is suspended below a buoyant float system at a predetermined depth. The apparatus is resistant to vertical motion by surface waves, and may provide additional aeration for egg species that require a high level of dissolved oxygen in order to incubate successfully. The apparatus may be constructed so as to provide maximum or minimum sunlight exposure to spawning bed nests and to accommodate the spawning depth and/or preferred bed slopes of certain fish species.

Abstract: A method of manipulating a spawning cycle of perch across multiple generations that includes identifying a desired out-of-cycle spawning period for perch, selecting a geographic strain of perch having a natural spawning period in proximity to the desired out-of-cycle spawning period, developing a first generation broodstock from the geographic strain, where the broodstock's natural spawning period has been shifted to the desired spawning period using at least one of temperature and photoperiod manipulation, identifying a second desired out-of-cycle spawning period, developing a second generation broodstock from the first generation broodstock, where the second generation broodstock's spawning period has been shifted from the desired out-of-cycle spawning period to the second desired out-of-cycle spawning period using at least one of temperature and photoperiod manipulation.

Abstract: A method for selecting a freshwater fish able to tolerate conditions of varying salinity is disclosed. Fish are first selected by pre-screening for salt-tolerance. The selected fish are bred using techniques essentially described herein. The offspring of the selected fish may then be subjected to additional factors to increase salt-tolerance. Thereafter, the offspring are reared in normal freshwater conditions resulting in a freshwater fish suitable for brief exposures to saltwater conditions.

Abstract: The present invention provides for a method for inducing sterility in fish by administering at least one compound that disrupts the establishment of the gonadotropin-releasing hormone system during early development thereby inhibiting sexual maturity in the treated fish. Effective compounds include GABA, GABA receptor agonists, or GABA receptor antagonists.

Abstract: The invention is a method and kit for conducting a rapid toxicity test. Methods and kits according to the invention include an animal or plant species in diapause.

Abstract: A high efficiency recirculating marine aquaculture process for producing fish at variable yield densities of up to 60 kg/meter3 of the aquaculture tank, including fish that spawn under short photoperiods such as gilthead seabream (Sparus aurata) as well as fish that spawn under long photoperiods such as striped bass (Morone saxatilis). The process involves broodstock conditioning, spawning/reproduction, larval growth, nursery, and adult grow-out operations. The process is characterized by high-rate growth at optimal process conditions requiring less than 10% daily water exchange, thereby enabling effluent waste from the system to be discharged to a municipal sewer after disinfection treatment, which in turn permits siting of the aquaculture process facility in urban/suburban locations where aquaculture processes have been previously infeasible.

Abstract: A method for manufacturing herring roe on kelp is described. The method comprises sandwiching kelp between layers of uncured herring roe in a mold made of a mesh material. The mold containing the roe and kelp is submerged in salt water such that the eggs adhere to the kelp and to each other. The outer surface of the eggs on kelp is textured to yield a natural appearance by dipping the eggs on kelp in a brine containing uncured eggs or by directing a stream of uncured eggs on the cured eggs on kelp until the desired appearance is obtained. Alternatively, a stream of uncured eggs can be directed against a piece of kelp originally having no eggs adhering to it until a layer of eggs builds up on the sides of the kelp to a selected thickness. Eggs on kelp prepared in this manner avoid contaminants, and the quality and freshness of the product can be controlled.

Abstract: A fish feed composition to reduce mortality and/or improve growth in juvenile salmon upon transfer to seawater. The fish feed includes L-carnitine in admixture with juvenile salmon feed compositions sufficient to improve gluconeogenesis and/or glycogen reserves during smoltification and adaption to seawater.

Abstract: A method and system are provided for using coal mine water effluent and for raising cold water fish. The method involves aerating coal mine water effluent having a water temperature of from 12 degrees Celsius to 15 degrees Celsius, and directing the water to a container having water and fish therein. The fish are preferably selected from rainbow trout, sturgeon, arctic char and Atlantic salmon. The system involves a coal mine having water effluent at a temperature of from 12 degrees Celsius to 15 degrees Celsius, an aeration device for aerating the water effluent to produce aerated water, means for carrying the water effluent from the mine to the aeration device, a container containing fish and water, and means for carrying the aerated water from the device to the container.

Abstract: A worm trap for use in an aquarium for trapping worms. The worm trap includes a tube assembly having a pair of openings at the ends of the assembly. A chamber is located within the assembly and is adapted to contain a food morsel. At least one end wall of the chamber communicates with the interior of the tube assembly to allow the aroma of the food morsel to enter the tube assembly so that a worm will be attracted to the aroma and be trapped in the tube assembly. The opening in the chamber is sufficiently small to prevent the food morsel from being carried out of the tube assembly by the worm.