Capillary Electrophoresis Patents (Class 204/451)
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Publication number: 20130240357Abstract: Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.Type: ApplicationFiled: February 12, 2013Publication date: September 19, 2013Applicant: BioNano Genomics, Inc.Inventors: Ming Xiao, Han Cao
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Publication number: 20130240355Abstract: A method for deionization of a solution, the method comprising functionalizing plural apertures of a graphene sheet to repel first ions in the solution from transiting through the functionalized plural apertures. The non-transiting first ions influence second ions in the solution to not transit through the functionalized plural apertures. The graphene sheet is positioned between a solution flow path input and a solution flow path output. Solution enters the solution flow path input and through the functionalized plural apertures of the graphene sheet, resulting in a deionized solution on the solution flow path output side of the graphene sheet and a second solution containing the first ions and second ions on the solution flow path input side of the graphene sheet.Type: ApplicationFiled: March 16, 2012Publication date: September 19, 2013Applicant: LOCKHEED MARTIN CORPORATIONInventors: Gregory S. Ho, Rex G. Bennett, Peter V. Bedworth, John B. Stetson, JR.
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Publication number: 20130240358Abstract: A method for making a surfactant-based monolithic column is provided. The method comprises providing a mixture comprising at least one surfactant monomer, at least one crosslinker, at least one initiator, and at least one porogen and polymerizing the mixture to form the surfactant-based monolithic column. The present disclosure also provides a surfactant-based monolithic column, a method for separating molecules, and a process for preparing a surfactant monomer.Type: ApplicationFiled: May 3, 2013Publication date: September 19, 2013Applicant: Gergia State University Research Foundation, Inc.Inventor: Georgia State University Research Foundation, Inc.
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Patent number: 8535500Abstract: Provided herein is a surface acoustic wave (“SAW”) sensor device including an isolation component of a target biomolecule. A sample containing the target biomolecule is separated by its size using electrophoresis, and sequentially reacts with a SAW sensor. In other words, the device is capable of detecting the target biomolecule by separating biomolecules using electrophoresis, and applying the separated biomolecules to the SAW sensor.Type: GrantFiled: December 21, 2010Date of Patent: September 17, 2013Assignees: Samsung Electronics Co., Ltd., Ajou University Industry-Academic Cooperation FoundationInventors: Hun Joo Lee, Soo Suk Lee, Mi Jeong Song, Kyung Yeon Han, Jae Ho Kim
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Publication number: 20130233709Abstract: Provided is a device comprising an upper chamber, a middle chamber and a lower chamber, wherein the upper chamber is in communication with the middle chamber through a first pore, and the middle chamber is in communication with the lower chamber through a second pore, wherein the first pore and second pore are about 1 nm to about 100 nm in diameter, and are about 10 nm to about 1000 nm apart from each other, and wherein each of the chambers comprises an electrode for connecting to a power supply. Methods of using the device are also provided, in particular for sequencing a polynucleotide.Type: ApplicationFiled: July 18, 2012Publication date: September 12, 2013Applicant: THE REGENT OF THE UNIVERSITY OF CALIFORNIAInventors: William Dunbar, Jungsuk Kim
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Publication number: 20130220810Abstract: A three-dimensional digital microfluidic system comprises a first plate with a first electrode, a second plate with a second electrode, and a microfluidic drop in between the first and the second electrode. The electrodes are able to be actuated in sequence such that the microfluidic drop is able to be transported. A bridge plate is able to be included.Type: ApplicationFiled: February 28, 2013Publication date: August 29, 2013Inventor: Gary Chorng-Jyh Wang
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Patent number: 8518226Abstract: The present invention provides for a novel method of supercoiled DNA isolation using a microfluidic device. In a preferred embodiment, the supercoiled DNA is a plasmid present in combination with chromosomal DNA. The present invention also provides for a novel method of mitochondrial DNA isolation using a microfluidic device.Type: GrantFiled: March 31, 2008Date of Patent: August 27, 2013Inventors: Christopher Backhouse, Dammika Manage, Iveta Sosova, Moira Glerum
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Patent number: 8518227Abstract: A nanoparticle translocation device includes a first reservoir having a first reservoir electrode, a second reservoir having a second reservoir electrode, and at least one nanopore providing fluid communication between the first and second reservoirs. The device also includes one or more inner electrode portions on an inner wall of the nanopore and one or more outer electrode portions disposed on an outer wall of the nanopore. The device further includes at least one DC voltage supply for selectively applying a DC voltage to each of the first reservoir electrode, the second reservoir electrode, and the outer electrode layer, where the inner electrode portions, the outer electrode portions, and the nanopore are in a substantially coaxial arrangement.Type: GrantFiled: September 15, 2011Date of Patent: August 27, 2013Assignee: Old Dominion University Research FoundationInventors: Shizhi Qian, Ali Beskok
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Publication number: 20130213815Abstract: A process for fabricating a nanochannel system using a combination of microelectromechanical system (MEMS) microfabrication techniques and atomic force microscopy (AFM) nanolithography. The nanochannel system, fabricated on either a glass or silicon substrate, has channel heights and widths on the order of single to tens of nanometers. The channel length is in the micrometer range. The nanochannel system is equipped with embedded micro or nanoscale electrodes, positioned along the length of the nanochannel for electron tunneling based characterization of nanoscale particles in the channel. Anodic bonding is used to cap off the nanochannel with a cover chip.Type: ApplicationFiled: February 15, 2013Publication date: August 22, 2013Inventors: Chao-Hung Steve Tung, Jin-Woo Kim
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Publication number: 20130213811Abstract: Systems and methods are provided for integrating the electrophoresis and blotting of samples. A capillary electrophoresis and blotting system allows concomitant electrophoretic separation and blotting to provide a rapid and simplified process. A microfluidic electrophoresis and blotting system provides electrophoretic separation in a microfluidic channel followed by electroblotting from the microfluidic channel to also provide a rapid and simplified process. These systems and methods can be used to assay smaller amounts of sample in less time than conventional processes, including conventional Western blotting techniques.Type: ApplicationFiled: August 23, 2012Publication date: August 22, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Robert Kennedy, Gwendolyn Anderson
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Publication number: 20130213809Abstract: A measuring system and method are disclosed for measuring an ion concentration in a process liquid using capillary electrophoresis. In at least one embodiment, the measuring system includes an inlet connectable to a process for supplying the process liquid; a throughflow system connected to the inlet; a capillary electrophoresis measuring device connected to the throughflow system and provided with a detector and measuring capillary; and a data processing unit connected operatively during use to the capillary electrophoresis measuring device.Type: ApplicationFiled: September 27, 2011Publication date: August 22, 2013Applicant: CAPILIX B.V.Inventors: Gerardus Antonius Johannes Besselink, Harm Rouwhorst
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Patent number: 8512538Abstract: This invention provides a capillary electrophoresis device in which capillaries are thermally regulated on a thermally responsive electrical path attached to an electrically insulating circuit board. This invention also provides an optical scanner useful for scanning an array of capillaries. A laser, optical detector and optical selector are in an arrangement that allows the optical detector to selectively detect an optical signal from any one or more of the plurality of electrophoresis capillaries.Type: GrantFiled: May 23, 2011Date of Patent: August 20, 2013Assignee: IntegenX Inc.Inventors: Lars Majlof, Ezra Van Gelder
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Publication number: 20130211113Abstract: Methods, systems, and apparatuses for extracting non-polar lipids from microalgae are achieved using a lipid extraction device having an anode and a cathode that forms a channel and defines a fluid flow path through which an aqueous slurry is passed. An electromotive force is applied across the channel at a gap distance in a range from 0.5 mm to 200 mm to cause the non-polar lipids to be released from the algae cells. The non-polar lipids can be extracted at a high throughput rate and with low concentrations of polar lipids such as phospholipids and chlorophyll.Type: ApplicationFiled: October 19, 2010Publication date: August 15, 2013Inventors: Nicholas Eckelberry, Michael Phillip Green, Scott Alexander Fraser
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Publication number: 20130210045Abstract: Ratios of measured values of glucose, citrate, and cis-aconitate in a biological sample obtained from a subject to measured values of glucose, citrate, and cis-aconitate in a biological sample obtained from a healthy subject are calculated, and the glucose ratio, the citrate ratio, and the cis-aconitate ratio are used to evaluate and/or assess fatigue. Similarly, an isocitrate ratio, a succinate ratio, a malate ratio, and a lactate ratio are calculated, and these ratios are used together with the three ratios to evaluate and/or assess fatigue. This makes it possible to objectively and easily diagnose and evaluate fatigue.Type: ApplicationFiled: August 22, 2011Publication date: August 15, 2013Applicants: RIKENInventors: Yosky Kataoka, Guanghua Jin, Yasuyoshi Watanabe, Seiki Tajima, Tomoyoshi Soga, Hirohiko Kuratsune, Emi Yamano
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Publication number: 20130183211Abstract: Non-limiting exemplary embodiment(s) of apparatus(es) and method(s) are described for the conveyance of fluid media and entrained materials between two or more locations, each possessing a different cross-sectional area. Equidistant pathways incorporated into uniquely designed conduits enable this transference to occur with minimal band spreading and separation resolution loss due to undesirable flow patterns that arise from end effects. The design enables the conduits to be employed with locations of almost any description including process channels, surfaces, or even open volume of any size and used for almost any purpose. The conduits and/or associated locations may be empty of any contents or filled with gelatinous, porous, granular, or particulated material. The design of non-limiting exemplary embodiment(s) of apparatus(es) and method(s) may be easily adapted or configured as necessary.Type: ApplicationFiled: January 12, 2012Publication date: July 18, 2013Inventor: Fred C. Senftleber
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Publication number: 20130177921Abstract: The present invention relates to methods of assessing damage to cellular DNA including the type, frequency and/or distribution of the DNA damage in the genome. The invention further provides methods of evaluating DNA damage in a cell caused by an agent and/or event as well as methods of determining a subject's prior exposure to an agent and/or event that is known or suspected to cause DNA damage. Further provided are methods of determining whether a subject is at an increased risk for a disease or disorder as a result of cellular DNA damage.Type: ApplicationFiled: November 26, 2012Publication date: July 11, 2013Applicant: The University of North Carolina at Chapel HillInventor: The University of North Carolina at Chapel Hill
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Publication number: 20130175170Abstract: Embodiments of electrophoresis systems, devices, and associated methods of analysis are described herein. In one embodiment, an electrophoresis device includes a first electrode having a first polarity, a second electrode having a second polarity, and a substrate. The substrate includes a first channel having a first section with a first cross-sectional area and a second section with a second cross-sectional area. The first end is electrically coupled to the first electrode, and the second end is electrically coupled to the second electrode. The first cross-sectional area is greater than the second cross-sectional area in a first dimension and in a second dimension generally orthogonal to the first dimension.Type: ApplicationFiled: February 10, 2012Publication date: July 11, 2013Applicant: WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATIONInventor: Cornelius F. Ivory
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Patent number: 8476063Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: GrantFiled: June 15, 2010Date of Patent: July 2, 2013Assignee: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga
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Publication number: 20130161192Abstract: An apparatus and method for linearly translocating nucleic acid molecules through an aperture at a reduced rate.Type: ApplicationFiled: August 10, 2012Publication date: June 27, 2013Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventors: Jeo-young SHIM, Dong-ho LEE, Tae-han JEON, Seong-ho CHO, Kun-sun EOM, Hee-jeong JEONG
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Patent number: 8470149Abstract: A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.Type: GrantFiled: December 5, 2011Date of Patent: June 25, 2013Assignee: Applied Biosystems, LLCInventors: Charles S. Vann, Debjyoti Banerjee, Timothy G. Geiser, James C. Nurse, Nigel P. Beard
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Patent number: 8473104Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected in the preferred structure of the provided methods and systems. In particular, the methods are structured into low-level device component control functions, middle-level actuator control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring, mixing, heating, and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations.Type: GrantFiled: July 22, 2011Date of Patent: June 25, 2013Assignee: Handylab, Inc.Inventors: Kalyan Handique, Karthik Ganesan, Sundaresh N. Brahmasandra
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Publication number: 20130153420Abstract: An apparatus and methods for concentrating samples for application to microfluidic devices are disclosed. The methods involve electrophoresing charged molecules from a high volume sample into a smaller volume. The analyte of interest can be a charged molecule or can be modified to be charged using, for example, one or more ionic moieties.Type: ApplicationFiled: June 28, 2012Publication date: June 20, 2013Inventor: Celine Hu
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Publication number: 20130153421Abstract: The invention relates to graft copolymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; also to ultra-high molecular weight poly(N,N-dimethylacrylamide) (“poly(DMA)”) polymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; and more particularly to supports, such as capillaries, containing these polymers and methods for separating biomolecules, especially polynucleotides, using capillary electrophoresis. The graft copolymers can be prepared by, e.g., grafting polyacrylamide units onto a poly(DMA) backbone. Separation media comprising such graft copolymers or ultra-high molecular weight poly(DMA) polymers yield superior performance in the analysis and separation of biomolecules by capillary electrophoresis.Type: ApplicationFiled: September 13, 2012Publication date: June 20, 2013Applicant: APPLIED BIOSYSTEMS, LLCInventor: Aldrich N.K. LAU
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Patent number: 8465637Abstract: The invention provides a method for the measurement of a concentration of a charged species in a sample, the sample having a plurality of types of charged species and at least one insoluble component. The method comprises: providing the sample on a surface of a partly permeable layer (30); allowing components of the sample to pass through the partly permeable layer (30) into a channel (12); and separating the components into sections, such that each at least one of the sections substantially comprises a single type of the plurality of the types of charged species, and determining the charge concentration in the at least one of the sections.Type: GrantFiled: November 21, 2006Date of Patent: June 18, 2013Assignee: Medimate Holding B.V.Inventors: Steven Selwyn Staal, Jan Floris, Albert Van Den Berg
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Publication number: 20130146459Abstract: This invention provides devices and apparatuses comprising the same and methods of use thereof for efficient pumping and/or mixing of relatively small volumes of fluid, wherein the fluid contains a sample within an inner fluid phase dispersed in an outer phase. Such devices utilize nonlinear electrokinetics as a primary mechanism for driving fluid flow and/or mixing the fluid. Methods of cellular analysis, drug delivery and others, utilizing the devices are described.Type: ApplicationFiled: June 15, 2010Publication date: June 13, 2013Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGYInventors: Martin Bazant, Manu Prakash
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Publication number: 20130146457Abstract: Provided herein are methods and systems pertaining to sequencing units of analytes using nanopores. In general, arresting constructs are used to modify an analyte such that the modified analyte pauses in the opening of a nanopore. During such a pause, an ion current level is obtained that corresponds to a unit of the analyte. After altering the modified analyte such that the modified analyte advances through the opening, another arresting construct again pauses the analyte, allowing for a second ion current level to be obtained that represents a second unit of the analyte. This process may be repeated until each unit of the analyte is sequenced. Systems for performing such methods are also disclosed.Type: ApplicationFiled: August 22, 2012Publication date: June 13, 2013Applicant: UNIVERSITY OF WASHINGTONInventors: Jens Gundlach, Ian M. Derrington, Marcus D. Collins
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Publication number: 20130146456Abstract: Provided herein are artificial membranes of mycolic acids. The membranes may be unsupported or tethered. These membranes are long lived and highly resistant to electroporation, demonstrating their general strength. The mycolic acid membranes are suitable for controlled studies of the mycobacterial outer membrane and can be used in other experiments, such as nanopore analyte translocation experiments.Type: ApplicationFiled: August 22, 2012Publication date: June 13, 2013Applicant: UNIVERSITY OF WASHINGTONInventors: Jens Gundlach, Ian M. Derrington, Kyle W. Langford
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Patent number: 8460530Abstract: A method of modifying the concentration of reactants and carrying out a chemical reaction on a microfluidic device in which first and second reactants are delivered into a reaction channel combined, the second reactant different from the first reactant and capable of reacting with the first reactant. The first reactant is subjected to a stacking process, thereby producing a first stacked reactant. The second reactant is subjected to the stacking process, thereby producing a second stacked reactant. The first stacked reactant is exposed to the second stacked reactant so that the first stacked reactant and the second stacked reactant undergo a chemical reaction.Type: GrantFiled: September 16, 2011Date of Patent: June 11, 2013Assignee: Caliper Life Sciences, Inc.Inventors: Charles Park, Irina Kazakova
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Publication number: 20130140180Abstract: A sheath-flow interface for producing electrospray from a capillary is provided. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. In the interface, electrokinetic flow moves a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The mixture of sheath liquid and analyte is directed to an electrospray emitter in order to generate an electrospray.Type: ApplicationFiled: June 4, 2012Publication date: June 6, 2013Applicant: University of Washington through its Center for CommercializationInventors: Norman Dovichi, Roza Wojcik
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Patent number: 8454811Abstract: A method for forming a microfluidic channel with improved flow characteristics for one or more analytes is disclosed. A microfluidic channel having modified surfaces is formed in fused silica. The fused silica surfaces are modified by the addition of a layer of borophosphosilicate glass. The addition of the borophosphosilicate glass results in an improved flow velocity profile of the analyte. As a result, control over the position and movement of analytes within the solution is improved.Type: GrantFiled: July 14, 2008Date of Patent: June 4, 2013Assignee: Octrolix BVInventor: Rene Gerrit Heideman
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Publication number: 20130134040Abstract: A disposable cartridge had body, bottom layer with first hydrophobic surface, top layer with a second hydrophobic surface, and gap between. The bottom layer is a flexible film sealingly attached at its circumference to the top layer. No spacer is between the layers. The top layer has loading sites for transferring processing liquids into the gap. In use, the bottom layer is a working film for manipulating samples in liquid droplets and is placed on an electrode array of a digital microfluidics system having base unit with cartridge accommodation site and electrode array at the site. The electrode array is supported by a bottom substrate that extends in a first plane and has individual electrodes. The system also includes a central control unit for controlling selection of the electrodes and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the gap by electrowetting.Type: ApplicationFiled: January 9, 2013Publication date: May 30, 2013Applicant: TECAN TRADING AGInventor: TECAN TRADING AG
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Publication number: 20130137589Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.Type: ApplicationFiled: September 10, 2010Publication date: May 30, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Rixun FANG, Manohar FURTADO, Manfred KAYSER, Kaye BALLANTYNE
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Publication number: 20130134039Abstract: A digital microfluidics system manipulates samples in liquid droplets within disposable cartridges and has disposable cartridges each with a bottom layer, a top layer and a gap therebetween. A base unit with cartridge accommodation sites and at least one electrode array with electrodes works with a cover plate at the sites and a control unit for controlling selection of the electrodes and for providing them with voltage pulses for manipulating liquid droplets within the cartridges by electrowetting. The cover plate has an electrically conductive material that extends parallel to the array. A selection of disposable cartridges and a method for manipulating samples in liquid droplets that adhere to a hydrophobic surface can be used with the system.Type: ApplicationFiled: November 25, 2011Publication date: May 30, 2013Inventors: Torleif Ove Bjornson, Marc Nathan Feiglin, Michael Benjamin Franklin, Anne R. Kopf-Sill, Travis Lee, Kailiang Wang
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Patent number: 8449880Abstract: Disclosed herein is a composition and method for sample preparation of proteins for their size separation by electrophoresis, suitable for molecular-weight determination of proteins in the range between about 14,000 and 500,000. In an embodiment, proteins, particularly those exhibiting biased migration, are modified to change their intrinsic charge, or carbohydrate component to improve accuracy of their molecular weights as determined by electrophoretic size separation via their interaction with ionic surfactants. In a preferred embodiment, the proteins are carbamylated with potassium cyanate and their carbohydrate components are oxidized with sodium periodate.Type: GrantFiled: November 22, 2010Date of Patent: May 28, 2013Inventors: Vladislav Dolnik, William A. Gurske
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Patent number: 8445223Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also described. The anionic acid-labile surfactants may be used to facilitate the solubilization of proteins and other molecules in an aqueous environment.Type: GrantFiled: June 25, 2012Date of Patent: May 21, 2013Assignee: Protea Biosciences, Inc.Inventors: Matthew Jacob Powell, Trust Tariro Razunguzwa, George Augustine O'Doherty, Miaosheng Li
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Publication number: 20130118901Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.Type: ApplicationFiled: January 9, 2013Publication date: May 16, 2013Applicant: DUKE UNIVERSITYInventors: Michael G. Pollack, Vamsee K. Pamula, Richard B. Fair
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Publication number: 20130112559Abstract: The invention relates to a microfluidic device and a method for handling at least one drop. The device comprises first and second microfluidic surfaces (3a, 3b) parallel and separated from each other by a separation distance (H), at least one first electrical displacement path (5a) arranged on said first surface (3a), and at least one second electrical displacement path (5b) arranged on said second surface (3b). The at least one of the first and second paths is configured in order to form a respective fluidic finger along said path, said fluidic finger rupturing via capillarity, by generating at least one respective drop. The first and second paths are configured so that said separation distance between said first and second surfaces is on the one hand, greater than the fluidic thickness formed by each fluidic finger and, on the other hand, less than the fluidic thickness formed by each drop.Type: ApplicationFiled: October 5, 2012Publication date: May 9, 2013Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVESInventor: Commissariat A L'Energie Atomique Et Aux Energies Alternatives
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Patent number: 8435795Abstract: Methods of evaluating heparin preparations, e.g., for suitability for use as a drug or for use in making a drug, by determining the absence, presence or amount of a structural signature that is indicative of the methods used to make the heparin preparation.Type: GrantFiled: January 18, 2011Date of Patent: May 7, 2013Assignee: Momenta Pharmaceuticals, Inc.Inventors: Daniela Beccati, Ishan Capila, Nur Sibel Gunay, Sucharita Roy
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Patent number: 8435756Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also described. The anionic acid-labile surfactants may be used to facilitate the solubilization of proteins and other molecules in an aqueous environment.Type: GrantFiled: June 25, 2012Date of Patent: May 7, 2013Assignee: Protea Biosciences, Inc.Inventors: Matthew Jacob Powell, Trust Tariro Razunguzwa, George Augustine O'Doherty, Miaosheng Li
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Patent number: 8435396Abstract: The invention provides methods for detecting an interaction between an analyte and a biomolecule. The method comprises separating at least one biomolecule according to its isoelectric point in the presence of a given analyte and detecting an interaction between the analyte and a biomolecule using fluorescence anisotropy. The method may further comprise collecting the analyte-biomolecule complex and analyzing the biomolecule.Type: GrantFiled: February 9, 2011Date of Patent: May 7, 2013Assignee: Southern Illinois University CarbondaleInventors: Luke Tolley, Matt McCarroll
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Publication number: 20130105317Abstract: An apparatus and method for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. Species movement is caused by a module array imparting opposing dielectrophoretic forces. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The Clausius-Mossotti factor of the analyte is changed by flushing the analyte with a reference solution, which causes a negative dielectrophoretic force to facilitate release of the analyte. A field effect nanowire or nanoribbon sensor detects the analyte after capture.Type: ApplicationFiled: October 31, 2012Publication date: May 2, 2013Inventors: Monika Weber, Siu Lung Lo, Hazael Fabrizio Montanaro Ochoa, Christopher Daniel Yerino, Mark A. Reed
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Publication number: 20130105319Abstract: Systems and methods are provided for producing fluids with desired concentration factors. According to one embodiment, an arrangement of digital microfluidic (DMF) based electrode platforms are provided. The arrangement may be configured to carry out a sequence of mix steps that may demand storage of resultant fluid mixtures produced in intermediate mix steps. Such sequences of mix steps may be desirable as a result of the decreased demand for initial fluid samples, and reduced wastage.Type: ApplicationFiled: November 12, 2010Publication date: May 2, 2013Applicant: INDIAN STATISTICAL INSTITUTEInventors: Bhargab B. Bhattacharya, Sudip Roy, Krishnendu Chakrabarty
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Publication number: 20130105316Abstract: Provided is a substrate modification method that enables improvement of reproducibility in measurement with use of capillary electrophoresis. The substrate modification method includes immobilizing, to a substrate surface, at least two types of modification groups selected from three types of modification groups that are a type of a modification group having one functional group, a type of a modification group having two to nine functional groups, and a type of a modification group having ten or more functional groups.Type: ApplicationFiled: October 26, 2012Publication date: May 2, 2013Applicant: ARKRAY, INC.Inventor: ARKRAY, INC.
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Publication number: 20130105314Abstract: The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.Type: ApplicationFiled: September 12, 2012Publication date: May 2, 2013Applicant: APPLIED BIOSYSTEMS, LLCInventors: Karl O. VOSS, Aldrich N. LAU
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Publication number: 20130109579Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.Type: ApplicationFiled: September 13, 2012Publication date: May 2, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Rixun FANG, Manohar Furtado, Manfred Kayser, Kaye Ballantyne
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Publication number: 20130105315Abstract: The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.Type: ApplicationFiled: September 13, 2012Publication date: May 2, 2013Applicant: APPLIED BIOSYSTEMS, LLC.Inventors: Karl O. VOSS, Aldrich N.K. Lau
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Publication number: 20130105318Abstract: Systems and methods are provided for producing fluids with desired concentration factors from the given supply of any two concentration factors, one greater than the target CF and one less than the target CF, of the same fluid. According to one embodiment, a method is provided that stores intermediate waste droplets from a sequence of mix and split steps and repeats certain steps of the sequence using the stored intermediate waste droplets. Such a method may produce additional target CF droplets faster than repeating the entire sequence. In another embodiment, a method of volumetric error resilient target CF droplet generation has been described, and includes reusing the stored intermediate waste droplets and involves a collection of capacitive sensing circuits associated with some electrode platforms.Type: ApplicationFiled: November 12, 2010Publication date: May 2, 2013Applicant: Indian Statistical InstituteInventors: Bhargab B. Bhattacharya, Sarmishtha Ghoshal, Sudip Roy, Krishnendu Chakrabarty
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Patent number: 8431340Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: GrantFiled: October 23, 2009Date of Patent: April 30, 2013Assignee: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga
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Patent number: 8431390Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: GrantFiled: November 2, 2011Date of Patent: April 30, 2013Assignee: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga
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Publication number: 20130095512Abstract: A biomolecule partitioning device (BPD) is provided that can be used to separate and sort membrane species into raft-like membrane regions without using detergent or crosslinkers. The BPD can comprise one or more microfluidic channels coated with coexistent lipid phases (raft-like and fluid-like lipid compositions) as a contiguous supported lipid bilayer (SLB). The geometry of the phases can be patterned with spatial and temporal control within each channel. Methods for the separation and sorting are also provided. The method can comprise the steps of introducing cell membrane species into an SLB; patterning coexistent phases; applying an electric field or hydrodynamic flow to move the species; sorting migrating species into regions based on their partitioning preference; and collecting sorted species in a quantification area. The BPD can also be used to measure partitioning kinetics or to assay for activity changes of biomolecules as a function of local lipid environment.Type: ApplicationFiled: June 17, 2011Publication date: April 18, 2013Applicant: Cornell UniversityInventors: Susan Daniel, Ling Chao