With Analysis Or Detailed Detection Patents (Class 204/461)
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Patent number: 11692937Abstract: Provided are a spectral calibration apparatus and a spectral calibration method capable of performing spectral calibration with high accuracy even when peaks appear simultaneously between fluorescent dyes. The spectral calibration apparatus for calculating a color conversion matrix used in color conversion processing, includes a spectral signal acquisition unit that acquires a spectral signal of fluorescence detected over time, a candidate calculation unit that calculates a candidate of the color conversion matrix for each value of a parameter, which depends on a frequency at which fluorescence peaks of fluorescent dyes appear at the same time, based on the spectral signal, and a selection unit that selects a color conversion matrix based on an evaluation value calculated for each candidate.Type: GrantFiled: August 9, 2019Date of Patent: July 4, 2023Assignee: HITACHI HIGH-TECH CORPORATIONInventors: Yohei Kawaguchi, George Chalkidis, Tomohiro Yasuda
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Patent number: 11536659Abstract: Laser emission based microscope devices and methods of using such devices for detecting laser emissions from a tissue sample are provided. The scanning microscope has first and second reflection surfaces and a scanning cavity holding a stationary tissue sample with at least one fluorophore/lasing energy responsive species. At least a portion of the scanning cavity corresponds to a high quality factor (Q) Fabry-Pérot resonator cavity. A lasing pump source directs energy at the scanning cavity while a detector receives and detects emissions generated by the fluorophore(s) or lasing energy responsive species. The second reflection surface and/or the lasing pump source are translatable with respect to the stationary tissue sample for generating a two-dimensional scan of the tissue sample. Methods for detecting multiplexed emissions or quantifying one or more biomarkers in a histological tissue sample, for example for detection and diagnosis of cancer, or other disorders/diseases are provided.Type: GrantFiled: December 27, 2017Date of Patent: December 27, 2022Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Xudong Fan, Yu-Cheng Chen, Qiushu Chen
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Patent number: 11403739Abstract: Methods and apparatus for retargeting and prioritized interpolation of lens profiles. A lens profile file may include a set of lens sub-profiles. The camera body and/or settings described in the file may not exactly match that of camera body and/or settings used to capture a target image. A sub-profile processing module may perform a prioritized sub-profile sorting and interpolation method to generate an interpolated sub-profile that may be applied to the target image to correct aberrations including, but not limited to, geometric distortion, lateral chromatic aberration, and vignette. Thus, models generated for a reference camera at a variety of settings may be applied to a target image captured with the same type of lens but with a different camera and/or with different settings that are not exactly modeled in the lens profile file.Type: GrantFiled: April 12, 2010Date of Patent: August 2, 2022Assignee: Adobe Inc.Inventors: Simon Chen, Eric Chan, Hailin Jin, Jen-Chan Chien
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Patent number: 11397140Abstract: This disclosure provides methods for producing size-adjustable tissue-hydrogel hybrids or cell-hydrogel hybrids for imaging cellular and subcellular details and system-scale (e.g., tissue or organism level) intercellular connectivity.Type: GrantFiled: April 28, 2017Date of Patent: July 26, 2022Assignee: Massachusetts Institute of TechnologyInventors: Kwanghun Chung, Taeyun Ku, Justin Mark Swaney, Jeong Yoon Park
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Patent number: 11391790Abstract: Disclosed are devices, systems and methods for assessing the integrity of electrical connections between elements of interfacing electronic devices. In some aspects, a system includes an analysis device having electronics that interface with an assay cartridge inserted into the analysis device, wherein the analysis device is configured to conduct a preflight test in which impedance values for each circuit between the assay cartridge and analysis device are rearranged and assessed to determine the electrical connection integrity of the assay cartridge to the analysis device prior to implementing the assay.Type: GrantFiled: August 17, 2020Date of Patent: July 19, 2022Assignee: Roche Molecular Systems, Inc.Inventors: Roger Harry Taylor, John B. Gorman, Tyler David Jensen
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Patent number: 11353424Abstract: Some embodiments provide methods and systems for creating ladder/standards as quality control tools for length-based separation of carbon nanotubes; determining the length purity; or measuring distribution of lengths of a collection of carbon nanotubes. Some embodiments further provide methods and systems for dispersing carbon nanotubes by conjugation of the carbon nanotubes with biomolecule moieties, specifically proteins. Further, some embodiments provide an indicator for length-based separation of carbon nanotubes via conjugation of one or more biomolecules onto the surfaces of the nanotubes. In some embodiments, such a method can include conjugating a biomolecule to the carbon nanotubes and subjecting the conjugated carbon nanotubes to silver-stained gel electrophoresis to separate the conjugated carbon nanotubes based on their lengths.Type: GrantFiled: October 22, 2019Date of Patent: June 7, 2022Assignee: Nano LC-12, LLCInventors: Zahra Borzooeian, Mohammad E. Taslim
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Patent number: 10983058Abstract: A device and method are described for the naked eye visualization of fluorophores. The device has a light source capable of exciting fluorophores, a rack to receive one or more fluorophores and a window through which test tubes can be observed. The method provides a means for inferring the conformational state of molecules from the amount of fluorescence emitted by the fluorophores.Type: GrantFiled: March 15, 2019Date of Patent: April 20, 2021Assignee: AMPLYUS LLCInventors: Ezequiel Alvarez Saavedra, Sebastian Kraves
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Patent number: 10883956Abstract: A sensor for the detection of an analyte in a biological sample includes a substrate, a working electrode and counter electrode formed on a surface of the substrate, and a receptor functionalized or chemically functionalized to a surface of an exposed portion of the working electrode. The receptor can selectively bind to the analyte of interest and the analyte once bound is detectable by measuring the current flow between the working electrode and counter electrode.Type: GrantFiled: May 26, 2015Date of Patent: January 5, 2021Assignee: CASE WESTERN RESERVE UNIVERSITYInventor: Chung-Chiun Liu
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Patent number: 10794802Abstract: In various embodiments, the present application teaches methods for tissue clearing in which tissues are rendered macromolecule-permeable and optically-transparent, thereby exposing their cellular structure with intact connectivity. In some embodiments, the present application teaches ePACT, which is a protocol for enhanced tissue clearing via expansion. In some embodiments, the present application teaches visualizing a tissue that has been expanded via ePACT.Type: GrantFiled: August 17, 2016Date of Patent: October 6, 2020Assignee: California Institute of TechnologyInventors: Viviana Gradinaru, Jennifer Treweek
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Patent number: 10697869Abstract: Provided herein are systems, devices, and methods for improved treatment of tissue, such as brain tissue. The improved treatment described herein can result in improved tissue penetration of various compounds and chemicals, such as stains and immunohistochemistry reagents. For example, provided herein is a pressurizing device that may include a chamber body having an opening in one of a top and a sidewall of the body, and may also include a chamber lid covering the opening and releasably coupled to the chamber body proximate the opening. The chamber lid and chamber body form an air-tight cavity. The pressurizing device may also have an inlet passing through one of the chamber body and the chamber lid and into the air-tight cavity. The device may also include a retainer coupled inside the air-tight cavity and configured to releasably couple to at least one tissue sample receptacles.Type: GrantFiled: April 29, 2019Date of Patent: June 30, 2020Assignee: Dignity HealthInventors: Roberto Fiorelli, Gurpaul Singh Sidhu
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Patent number: 10641731Abstract: An electrophoresis running tank assembly uses two opposed rows of LEDs to illuminate DNA-containing gel on a transparent tray positioned between the rows. A respective cylindrical lens is positioned horizontally between each row and a respective edge of the tray. The optical axis of the illumination light is midway between a bottom surface of the gel tray and a top surface of the gel.Type: GrantFiled: October 24, 2017Date of Patent: May 5, 2020Assignee: C.C. IMEXInventors: Winston Glenn Walker, Richard Chan, Rita M. Wong, Ramey K. Chan, Po Ling Cheng
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Patent number: 10559404Abstract: It is disclosed a power cable comprising at least one conductor and a hollow tube at least partially filled with a traceable material. The traceable material comprises a tracer associated with a uniquely identifiable code and is in a liquid or gel form. The tracer may comprise one or more of: coded synthetic DNA particles, a fingerprint of one or more trace materials, microdots containing a code written thereon. The tracer may also comprise radio-frequency identification, RFID, tags.Type: GrantFiled: June 8, 2017Date of Patent: February 11, 2020Assignee: PRYSMIAN S.p.A.Inventors: Andreas Neumann, Frank Lübbe
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Patent number: 10495554Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.Type: GrantFiled: May 25, 2017Date of Patent: December 3, 2019Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Karl A. Deisseroth, William E. Allen, Brian Hsueh, Li Ye
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Patent number: 10473658Abstract: The invention relates to a family of compounds that comprise fluorescent cyanine dyes. The compounds are near infrared absorbing heptamethine cyanine dyes with a 4,4-disubstituted cyclohexyl ring as part of the polymethine chromophore. The compounds are generally hydrophilic and can be chemically linked to biomolecules, such as proteins, nucleic acids, and therapeutic small molecules. The compounds can be used for imaging in a variety of medical, biological and diagnostic applications.Type: GrantFiled: February 7, 2018Date of Patent: November 12, 2019Assignee: VisEn Medical, Inc.Inventor: Narasimhachari Narayanan
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Patent number: 10082453Abstract: An airborne microbial measurement apparatus and a measurement method thereof are provided. An airborne microbial measurement apparatus according to an embodiment includes a discharge apparatus including a discharge electrode and a voltage supply unit applying a high voltage to the discharge electrode. A substrate is provided to a side of the discharge apparatus to collect an airborne microbe from air by a high voltage applied to the discharge electrode. A reagent injection apparatus supplies a dyeing reagent to the microbe collected on the substrate or a DNA of the microbe. A light emission measurement apparatus senses a quantity of light generated from the DNA to which the dyeing reagent is supplied. The discharge apparatus includes a controller controlling the voltage supply unit so that the voltage is applied to collect the airborne microbe or destroy an external wall of the collected airborne microbe.Type: GrantFiled: September 19, 2014Date of Patent: September 25, 2018Assignees: LG ELECTRONICS INC., NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGYInventors: Chulwoo Park, Sunghwa Lee, Akira Mizuno, Kazunori Takashima, Hirofumi Kurita, Hachiro Yasuda
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Patent number: 10043054Abstract: Methods, systems, and devices for classifying a biological sample that include receiving an image of a biological sample and applying one or more algorithms from a data repository to the image, generating a classification of the biological sample based on the outcome of the one or more algorithms applied to the image, and transmitting the classification for presentation on a display or via another medium. The methods, systems, and devices may also include features for developing a data master reference and/or other correlation/translation features to enable comparison of data sets from one platform to another or from one machine to another or from the same machine at different points in time.Type: GrantFiled: October 19, 2015Date of Patent: August 7, 2018Assignee: CIRECA THERANOSTICS, LLCInventors: Stanley H. Remiszewski, Max Diem, Clay M. Thompson, Aysegul Ergin, Xinying Mu, Mark Kon
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Patent number: 9897604Abstract: The invention relates to a family of compounds that comprise fluorescent cyanine dyes. The compounds are near infrared absorbing heptamethine cyanine dyes with a 4,4-disubstituted cyclohexyl ring as part of the polymethine chromophore. The compounds are generally hydrophilic and can be chemically linked to biomolecules, such as proteins, nucleic acids, and therapeutic small molecules. The compounds can be used for imaging in a variety of medical, biological and diagnostic applications.Type: GrantFiled: March 17, 2014Date of Patent: February 20, 2018Assignee: VisEn Medical, Inc.Inventor: Narasimhachari Narayanan
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Patent number: 9752178Abstract: Methods and compositions for determining the nucleic acid sequence of polynucleotides that are at least 1500 nucleotides in length are provided.Type: GrantFiled: September 26, 2014Date of Patent: September 5, 2017Assignee: President and Fellows of Harvard CollegeInventors: Chao-ting Wu, George M. Church
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Patent number: 9663554Abstract: Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate.Type: GrantFiled: September 17, 2015Date of Patent: May 30, 2017Assignee: LI-COR, Inc.Inventors: Nisha Padhye, Rose Skopp, Ying Wang
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Patent number: 9494552Abstract: Disclosed in this specification is a high capacity compact gel documentation system for documenting different types of electrophoresis gels or other translucent objects using ultraviolet light. The system includes a base with a scanning surface having a transparent bottom surface. A light source is connected to a conveying mechanism, disposed below the transparent bottom surface, to move the light source over the length of the transparent bottom surface. An image capture device receives a reflected image and provides it to a microprocessor. Moreover, separate interchangeable filters allow for documentation of UV and white light gel captures, alleviating the need for separate transilluminators and hoods with filters and cameras.Type: GrantFiled: February 7, 2013Date of Patent: November 15, 2016Assignee: Research Foundation of The City University of New YorkInventors: Farshad Tamari, Farnaz Tamari
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Patent number: 9488616Abstract: Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment.Type: GrantFiled: May 17, 2013Date of Patent: November 8, 2016Assignee: Bio-Rad Laboratories, Inc.Inventor: Christopher Belisle
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Patent number: 9428806Abstract: Systems and methods for specific nucleic acid (NA) sequence detection that do not rely on polymerase chain reaction (PCR) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.Type: GrantFiled: February 25, 2014Date of Patent: August 30, 2016Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Harold G. Monbouquette, Jacob J. Schmidt
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Patent number: 9279152Abstract: Sample preparation processes for in situ RNA or DNA analysis, methods and compositions therefor are provided. Processes provided herein allow DNA or RNA analysis to be carried out in the same tube or on an aliquot of the prepared sample without centrifugation or extraction. The preparation process can be carried out at room temperature in as little as seven minutes and is amenable to high throughput processing using manual or robotic platforms.Type: GrantFiled: August 14, 2014Date of Patent: March 8, 2016Assignee: Applied Biosystems, LLCInventors: Richard Fekete, Annalee Nguyen
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Patent number: 9164039Abstract: A method includes calibrating color bleed factors of optical detector channels of a sample processing apparatus through processing a color bleed calibration substance which includes a plurality of different size fragments replicated from different groups of DNA loci, wherein fragments in a same group are labeled with a same fluorescent dye, and fragments in different groups are labeled with different fluorescent dyes having different emission spectra, wherein the different size fragments are processed during different acquisition times.Type: GrantFiled: October 20, 2010Date of Patent: October 20, 2015Assignee: Analogic CorporationInventor: Ching Ming Lai
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Patent number: 9097663Abstract: The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. In some embodiments, the electrophoresis system may analyze an imaged gel during or after electrophoresis. The device may comprise a gel processing system, a gel illumination system, an image capture system, and an image analysis all housed within a housing.Type: GrantFiled: December 17, 2012Date of Patent: August 4, 2015Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Timothy Updyke, Paul Predki, Evangeline Gonzalez, Randall Lowe, Tad Simons
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Publication number: 20150144490Abstract: Methods and compositions are provided for preparing a biological specimen for microscopic analysis. These methods find many uses, for example in medicine and research, e.g., to diagnose or monitor disease or graft transplantation, to study healthy or diseased tissue, to screen candidate agents for toxicity and efficacy in disease modification. Also provided are reagents, devices, kits and systems thereof that find use in practicing the subject methods.Type: ApplicationFiled: March 13, 2013Publication date: May 28, 2015Applicant: The Board of Trustees of the Leland Standford Junior UniversityInventors: Karl A. Deisseroth, Kwanghun Chung
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Patent number: 9005418Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.Type: GrantFiled: August 7, 2013Date of Patent: April 14, 2015Assignee: Bio-Rad Laboratories, Inc.Inventor: Christopher Belisle
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Publication number: 20150083594Abstract: An electrophoresis controller for use with an electrophoresis apparatus having a gel matrix disposed between electrodes for separation of particles along with a tracking dye. The electrophoresis controller includes a sensor system and a controller. The sensor system includes a support, a light emitter, and a light receiver. The support includes a first portion positionable on a first side of the gel matrix and a second portion positionable adjacent a second side of the gel matrix. The light emitter is positioned on the first portion of the support for emitting light onto one side of the gel matrix. The light receiver is positioned on the second portion of the support adjacent to the other side of the gel matrix for receiving light from the light source as it is passing through the gel matrix. At least one of the light emitter and the light receiver includes a light guide having a first end and a second end.Type: ApplicationFiled: October 22, 2014Publication date: March 26, 2015Applicant: THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORKInventors: Papa Nii ASARE-OKAI, Rabi A. MUSAH, Tony P. HOANG
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Patent number: 8974651Abstract: The present invention discloses apparatuses and methods for the visualization of fluorophores in biological systems such as electrophoresis gels and cell cultures. Preferred embodiments of the apparatuses consists of a tray or tank having one or more light sources disposed to direct light through an electrophoresis gel disposed within the vessel such that the luminescence from fluorophores in the gel are easily visualized.Type: GrantFiled: April 17, 2011Date of Patent: March 10, 2015Assignee: C.C. IMEXInventors: Richard Chan, Winston Glenn Walker, Rita M. Wong, Jason In
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Patent number: 8974731Abstract: A sample processing apparatus includes a sample carrier receiving region configured to receive a sample carrier. The sample carrier includes at least one sample channel carrying at least one sample, at least one agent chamber carrying at least one agent to be moved to the at least one sample channel to facilitate processing of the at least one sample, and the at least one agent chamber includes at least one chamber cover covering at least one opening of the at least one agent chamber, inhibiting flow of the at least one agent from the at least one agent chamber to the at least one sample channel. The sample processing apparatus further includes a chamber opener configured to facilitate opening the at least one chamber cover. The sample processing apparatus further includes a fluid mover that moves the agent out of the at least one agent chamber after the at least one chamber cover is opened and into the at least one sample channel.Type: GrantFiled: August 4, 2011Date of Patent: March 10, 2015Assignee: Analogic CorporationInventor: Ari Eiriksson
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Publication number: 20150060280Abstract: Jatropha lines can be classified and discriminated by a method including the steps of: conducting a nucleic acid amplification reaction using a primer set including (i) adjacent forward primer and (ii) adjacent reverse primer, and (iii) LTR forward primer or LTR reverse primer, or (iv) RTP forward primer or RTP reverse primer, wherein DNA prepared from Jatropha that is an objective of determination, is used as a template; and determining the presence or absence of insertion of LTR-type retrotransposon including the retrotransposon sequence used in (iii) or (iv), on the basis of the presence or absence and length of an amplification product obtained by the amplification reaction.Type: ApplicationFiled: August 29, 2014Publication date: March 5, 2015Inventors: Kiichi FUKUI, Suguru TSUCHIMOTO, Hiroe SAKAI, Atefeh ALIPOUR, Toshifumi HOSOYA, Yasuhisa YUSHIO
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Publication number: 20150060279Abstract: The present embodiments provide systems, kits and methods suitable for performing dry or substantially dry electro-blotting analyses on immobilized protein or nucleic acid samples. Electro-blotting performed according to the presently described embodiments may include a step whereby detection of one or more immobilized proteins or nucleic acids is electrophoretically accelerated. Methods for performing electro-blotting of immobilized proteins or nucleic acids may include applying an electric voltage to one or more reagents typically used in protein or nucleic acid blotting procedure. The one or more reagents may be absorbed on a suitable carrier matrix. Electro-blotting performed in accordance with the systems and methods described herein may be performed under substantially dry conditions (i.e., with little or no aqueous buffers).Type: ApplicationFiled: August 11, 2014Publication date: March 5, 2015Inventors: Ilana MARGALIT, Galia Feldman, Svetlana Lifshits
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Publication number: 20150050673Abstract: The present invention is directed to a method of detecting intact fibrinogen, comprising the steps of: a) providing a sample containing at least some fibrinogen optionally converted at least in part to fibrin, and optionally containing thrombin; b) solubilizing the sample in a solubilizing solution that inhibits thrombin activity; c) after optional SDS-PAGE transferring/applying a portion of said sample to a protein-binding membrane; d) reacting the fibrinogen with a primary monoclonal antibody capable of binding to fibrinopeptide A moiety; and e) detecting the quantity of intact fibrinogen in the sample by quantifying the amount of the bound primary monoclonal antibody.Type: ApplicationFiled: October 29, 2014Publication date: February 19, 2015Inventors: Ashley DeAnglis, Elif Burcoglu
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Publication number: 20150047980Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.Type: ApplicationFiled: October 28, 2014Publication date: February 19, 2015Applicant: Caliper Life Sciences, Inc.Inventors: Dan Camporese, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
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Publication number: 20140311909Abstract: Disclosed herein is a protein standard for gel electrophoresis. The standard may be detected using multiple modalities. These modalities include, for example, observation of color or fluorescence arising from dyes, porphyrins, or haloalkylated tryptophan residues covalently linked to proteins of the standard; or detection of fluorescence or chemiluminescence arising from antibodies or other binding partners bound to proteins of the standard through polypeptide epitopes or affinity tags. The protein standard comprises multiple protein sets, each set corresponding to a different gel band, and proteins within a set may be detectable with one or more modalities.Type: ApplicationFiled: March 13, 2014Publication date: October 23, 2014Applicant: Bio-Rad Laboratories, IncInventors: Joseph S. Siino, JR., Dennis C. Yee, Robin Baginski
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Publication number: 20140202859Abstract: Apparatus and methods for size selecting nucleic acid molecules having wide range of applications including the production of DNA libraries for sequencing technologies. An automated high throughput system for size selection of multiple nucleic acid samples that uses imaging technique to detect the progress of a target fraction and feedback from the imaging to control electrophoresis. Predictive algorithms for timed nucleic acid extractions are generated to provide size selected nucleic acid molecules of required size ranges.Type: ApplicationFiled: June 15, 2012Publication date: July 24, 2014Applicant: BRITISH COLUMBIA CANCER AGENCY BRANCHInventors: Robin J. Noel Coope, Jared Raymond Slobodan
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Publication number: 20140186822Abstract: Methods for the detection of biologically relevant molecules that comprise concentrating such molecules into microscopic holes in a sheet of chemically inert material, restricting the openings, and measuring the electric current through the holes or the fluorescence near the hole openings. The electric current or fluorescence will change as the molecules diffuse out of the holes, providing a measure of the diffusion rate and thereby detecting the presence and characteristics of the molecules. For molecules that interact, the diffusion rate will be slower than for molecules that do not interact, yielding a determination of the molecular interaction. Capping the population of holes and inserting into a mass spectrometer allows identification of the molecules.Type: ApplicationFiled: March 4, 2014Publication date: July 3, 2014Inventor: Christopher Gordon ATWOOD
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Publication number: 20140076726Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.Type: ApplicationFiled: May 15, 2013Publication date: March 20, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Matthew BEAUDET, William Gregory Cox, Stephen Yue
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Publication number: 20140042027Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.Type: ApplicationFiled: August 7, 2013Publication date: February 13, 2014Applicant: Bio-Rad Laboratories, Inc.Inventor: Christopher Belisle
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Publication number: 20130319864Abstract: The invention relates to, among other things, a method for performing electrophoresis with in-situ calibration. The method includes combining a volume of a test sample with a volume or quantity of a calibrating sample to form a final volume, where the volume of the calibrating sample includes a known concentration of a calibrator and the final volume includes a known ratio of test sample to calibrating sample. The method also includes depositing a loading fraction in a receiving well of an electrophoretic gel, in which the loading fraction is a fraction of the final volume, and separating the loading fraction along a common separation lane of the electrophoretic gel such that components of the test sample and the calibrator are separated from one another along the common separation lane.Type: ApplicationFiled: May 29, 2013Publication date: December 5, 2013Inventors: Philip GUADAGNO, Erin SUMMERS
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Publication number: 20130306854Abstract: Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment.Type: ApplicationFiled: May 17, 2013Publication date: November 21, 2013Inventor: Christopher Belisle
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Patent number: 8557609Abstract: A method employing gel electrophoresis and optical imaging techniques to measure the amount of biomaterial that attaches to specified locations on a detector slide such as a bioarray or biochip.Type: GrantFiled: October 18, 2010Date of Patent: October 15, 2013Assignee: Maven Biotechnologies, LLCInventors: Shane Dultz, David Ralin, William Rassman
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Publication number: 20130210648Abstract: Compositions, methods and kits are described for identifying biomolecules (e.g., proteins and nucleic acids) expressed in a biological sample that are associated with the presence, development, or progression of a disease (such as cancer), or more generally determination of the etiology or risk factors associated with a disease. Sample types analyzed by the disclosed methods include but are not limited to archival tissue blocks that have been preserved in a fixative, tissue biopsy samples, tissue microarrays, and so forth. The methods disclosed herein correlate expression profiles of biomolecules with various disease types, and allow for the determination of relative survival rates; in some embodiments, the methods permit determination of survival rates for a subject with cancer. In other embodiments, the disclosure relates to methods for evaluating therapeutic regimes for the treatment, such as treatment of cancer.Type: ApplicationFiled: March 15, 2013Publication date: August 15, 2013Inventor: The United States of America, as represented by the Secretary, Department of Health and Human Services
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Publication number: 20130199930Abstract: Disclosed in this specification is a high capacity compact gel documentation system for documenting different types of electrophoresis gels or other translucent objects using ultraviolet light. The system includes a base with a scanning surface having a transparent bottom surface. A light source is connected to a conveying mechanism, disposed below the transparent bottom surface, to move the light source over the length of the transparent bottom surface. An image capture device receives a reflected image and provides it to a microprocessor. Moreover, separate interchangeable filters allow for documentation of UV and white light gel captures, alleviating the need for separate transilluminators and hoods with filters and cameras.Type: ApplicationFiled: February 7, 2013Publication date: August 8, 2013Applicant: Research Foundation of The City University of New YorkInventor: Research Foundation of The City University of New York
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Publication number: 20130157282Abstract: Systems, devices, and methods for accurately imaging chemiluminescence and other luminescence are disclosed. A compact, flat-bed scanner having a light-tight enclosure, one or more detector bars of linear charge-coupled device (CCD) or complementary metal oxide semiconductor (CMOS) imaging chips, and high working numerical aperture (NA) optics scans closely over a sample in one direction and then the opposite direction. Averages or other combinations of intensity readings for each pixel location (x, y) between the two or more passes are averaged together in order to compensate for luminescence that varies over time. On-chip pixel binning and multiple clock frequencies can be used to maximize the signal to noise ratio in a CCD-based scanner.Type: ApplicationFiled: March 28, 2012Publication date: June 20, 2013Applicant: Li-Cor, Inc.Inventors: Ahmed Bouzid, Chris Lesiak, Daniel W. Konz, David L. Franzen, William Biggs, Jon P. Anderson
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Publication number: 20130130236Abstract: A method for separating labeled cells and a use (of the labeled cells) thereof are provided. More specifically, a method for labeling cells using fluorescent magnetic nanodiamonds, and a method for separating the labeled cells using the labeling method by the fluorescent or magnetic properties of the nanodiamonds are provided.Type: ApplicationFiled: May 15, 2012Publication date: May 23, 2013Applicant: NATIONAL CHIAO TUNG UNIVERSITYInventors: Jui-I Chao, Zhi-Yi Lien, Kuo-Chu Hwang
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Publication number: 20130105320Abstract: A slide holder assembly and method for use in high content screening of a comet assay. The slide holder assembly has an inside surface and is configured to receive a slide holding a plurality of biological cells on a top surface thereof. When the slide is secured within the slide holder assembly, the inside surface of the slide holder assembly and the top surface of the slide combine to form a cavity having an open top end. The cavity is watertight and configured to hold a liquid therein. A method of performing a comet assay using a slide disposed within a slide holder assembly. The method includes performing encapsulation, lysis, electrophoresis, staining, and imaging of cells on the slide while the slide remains secured within the slide holder assembly.Type: ApplicationFiled: October 23, 2012Publication date: May 2, 2013Applicant: CELLOMICS, INC.Inventor: Cellomics, Inc.
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Patent number: 8431339Abstract: The present invention relates to a method for purifying analyte molecules and in particular to a component of this type in which a separation section is used for separating analyte molecules and other constituents of a sample, and in which provision is made of at least one sample chamber for receiving a sample containing the analyte molecules and at least one collecting chamber for receiving the purified analyte molecules. According to the invention, the microfluidic component has at least one integrated receptor device for detecting the presence and/or the concentration of the purified analyte molecules. In accordance with one advantageous development of the present invention, the separation section is formed by an electrophoretic gel filtration section.Type: GrantFiled: October 26, 2007Date of Patent: April 30, 2013Assignee: Albert-Ludwigs-Universitat FreiburgInventors: Paul Vulto, Gerald Urban
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Publication number: 20130053254Abstract: The present invention relates to a process for the parallel isolation and/or purification of RNA and DNA from the same fixed biological sample, the quantification and analysis of the nucleic acids isolated by the process according to the invention, to a kit for the parallel isolation and/or purification of RNA and DNA from a fixed sample and to the use of this kit for the diagnosis, prognosis, decision with respect to therapy and/or the monitoring of the therapy of a disease.Type: ApplicationFiled: February 25, 2011Publication date: February 28, 2013Applicant: QIAGEN GmbHInventor: Vera Holländer
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Publication number: 20130032481Abstract: A sample processing apparatus includes a sample carrier receiving region configured to receive a sample carrier. The sample carrier includes at least one sample channel carrying at least one sample, at least one agent chamber carrying at least one agent to be moved to the at least one sample channel to facilitate processing of the at least one sample, and the at least one agent chamber includes at least one chamber cover covering at least one opening of the at least one agent chamber, inhibiting flow of the at least one agent from the at least one agent chamber to the at least one sample channel. The sample processing apparatus further includes a chamber opener configured to facilitate opening the at least one chamber cover. The sample processing apparatus further includes a fluid mover that moves the agent out of the at least one agent chamber after the at least one chamber cover is opened and into the at least one sample channel.Type: ApplicationFiled: August 4, 2011Publication date: February 7, 2013Applicant: ANALOGIC CORPORATIONInventor: Ari Eiriksson