Abstract: A cartridge, in particular a disposable cartridge, for use in an electrowetting sample processing system. The cartridge has a liquid input port for introducing an input liquid into an internal gap of the cartridge, the input liquid providing for at least one droplet, directly or via a liquid separation process within the internal gap, and the internal gap having at least one hydrophobic surface, at least one processing zone for processing samples located in the processing zone, and a delivery zone for delivering the at least one droplet from the liquid input port to the at least one processing zone. The delivery zone is configured to provide a repeating pattern of interacting electrowetting force for simultaneously transporting the at least one droplet within the delivery zone.

Abstract: Method and systems for identifying and distinguishing subjects using a detection system comprising pooled probes are described. The pooled probes described comprise subject specific features allowing for identification and distinction of subjects in a complex sample and can be utilized in a variety of detection platforms. The methods and systems can further comprise the use of isotachophoresis to concentrate analytes in a sample.

Abstract: An instrument for biological analysis includes a base, an excitation source, an optical sensor, an excitation optical system, and an emission optical system. The base is configured to receive a sample holder comprising a plurality of biological samples. The optical sensor is configured to receive emissions from the biological samples in response to the excitation source. The instrument may additionally include a sensor lens enclosed by a lens case and a focusing mechanism including a gear that engages the lens case, the focusing mechanism being accessible outside the enclosure for adjusting a focus. The may instrument further include a sensor aperture dispose along an emission optical path and a blocking structure disposed to cooperate with the sensor aperture such that none of the reflected radiation from an illuminated surface near the sample holder is received by the optical sensor that does not also reflect off another surface of the instrument.

Abstract: Embodiments described herein generally relate to cartridges suitable for performing electrophoretic separation of analytes. Cartridges described herein are particularly well suited for reuse. Cartridges described herein can include a reservoir disposed between a capillary and a container containing a run buffer. The reservoir can inhibit run buffer from intruding into the capillary, thereby allowing repeated electrophoretic separations to be more consistent, more accurate, and/or more reliable.

Abstract: Method and systems for identifying and distinguishing subjects using a detection system comprising pooled probes are described. The pooled probes described comprise subject specific features allowing for identification and distinction of subjects in a complex sample and can be utilized in a variety of detection platforms. The methods and systems can further comprise the use of isotachophoresis to concentrate analytes in a sample.

Abstract: Aspects of the disclosure relate to using synthetic DNA stranding and mutant nucleotide processes to conduct enterprise market volatility predictions. In some embodiments, a computing platform may receive market data from a plurality of lines of business across an enterprise, wherein the market data is received in a raw, uncompressed format. Thereafter, the computing platform may assimilate and preprocess the market data to output vectored market data. The computing platform may perform a synthetic DNA stranding process on the vectored market data to create one or more strands of synthetic DNA market data, and output the one or more strands of synthetic DNA market data to a synthetic DNA client server, wherein the one or more stands of synthetic DNA market data is configured for input in a market volatility prediction model.

Abstract: Devices for improved nanopore sensing are described. An example device has a structure arranged to separate an analyte reservoir and an outlet chamber. An example device has a structure arranged to separate an analyte reservoir and an outlet chamber. The structure can include an array of nanopore structures, each nanopore structure comprising a passage for fluid connection through the structure between the analyte reservoir and outlet chamber. Control terminals can be arranged for applying a control signal to alter the electrical potential difference across that nanopore structure. Some embodiments include an electronic circuit configured to detect a signal from an electrical transduction element at each nanopore structure. Additional structural features and methods of operating and making the devices are described.

Abstract: A capillary array electrophoresis (CAE)-chemiluminescence (CL) detection coupled system includes a high-voltage power supply, a capillary array, an array channel CL reaction tank, a CAE sample tank, a CAE detection tank, a chemiluminescent reagent delivery unit, a multi-channel detection unit, and a data acquisition and processing unit. An inlet end of the capillary array is connected to the CAE sample tank. An outlet end of the capillary array is connected to the array channel CL reaction tank, and is further connected to the CAE detection tank.

Abstract: The capillary electrophoresis apparatus according to the present invention can maintain the temperature in the longitudinal direction of each of a plurality of capillaries uniformly, such that the separation performance of the capillary electrophoresis apparatus can be stabilized, and the analysis performance can be improved.

Abstract: An electrospray emitter assembly for interfacing a separation column to a mass spectrometer is disclosed. An emitter capillary includes an inlet end and an outlet end. A fitting is coupled to the inlet end of the emitter, configured to be removably connected to the separation column. A stop with a defined through hole is integrated proximate the inlet end of the emitter to produce a path for liquid to flow from the separation column to the emitter via the through hole where a voltage is applied to the liquid entering the emitter.

Abstract: In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.

Abstract: Provided is a capillary array unit configured to facilitate attaching/detaching operation. A capillary array unit includes a capillary, a load header provided at one end of the capillary, a capillary head provided at the other end of the capillary, a detection section provided at a portion of the capillary, and a holder holding the capillary. The holder includes a first holding section holding the capillary in a curved shape, a second holding section linearly holding the capillary, and a guide to move the second holding section in a predetermined direction.

Abstract: A system and methods of characterizing a population of a virus, bacteriophage, or other microbes in a microbiome that includes the steps of separating a sample of microbiota into more than one fraction by continuous capillary zone electrophoresis based on the physiochemical properties of the microbes in the microbiota using a constant voltage applied to the sample during the continuous zone electrophoresis. At least one separated fraction includes an intact virus, bacteriophage, or other microbe that may be visualized and/or directly sequenced to characterize the population of the virus, bacteriophage, or other microbe in the microbiome.

Abstract: The present invention aims to provide an electrophoresis apparatus which makes it possible to execute protein analysis with a high throughput. The electrophoresis apparatus according to the present invention is equipped with a capillary array which is configured by arraying a plurality of capillaries, a measurement light irradiation unit which irradiates with measurement light, a first lens array which includes a plurality of first lenses which are arrayed in correspondence with the plurality of capillaries, a second lens array which includes a plurality of second lenses which are arrayed in correspondence with the plurality of capillaries, and a light receiving unit which receives light which is incident upon the capillaries via the first lens array from the measurement light irradiation unit via the second lens array.

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

Abstract: An implantable diagnostic device in accordance with the present disclosure provides various benefits such as a compact size thereby allowing implanting of the device inside animate objects; low cost due to incorporation of inexpensive detection circuitry and the use of conventional IC fabrication techniques; re-usability by heating thereby allowing multiple diagnostic tests to be performed without discarding the device; and a configuration that allows performing of simultaneous and/or sequential diagnostic tests for detecting one or more similar or dissimilar target molecules concurrently or at different times.

Abstract: A capillary cartridge achieves both improvement of attachability and improvement of heat dissipation performance for realizing short-time analysis. A heat dissipation body is provided between a capillary having a detection unit provided in a part thereof and a plate-like support body that supports the capillary, and temperature increase inside the capillary is suppressed by the heat dissipation body, and thereby, electrophoresis can be performed under a high voltage application condition where the amount of heat increases and analysis time is reduced. In addition, it is possible to redress complexity of an operation by reducing a fixing place at the time of attachment to only a detection unit and an electrode holder by using an integration structure in which the capillary, the supporting body, and the heat radiating body are integrated.

Abstract: A sample observation device includes an irradiation unit that irradiates a sample with planar light, a scanning unit that scans the sample in one direction with respect to an irradiation surface of the planar light, an image formation unit that forms images of fluorescent light and scattered light from the sample, an imaging unit that outputs first image data based on a light image of the fluorescent light and second image data based on a light image of the scattered light, an image processing unit that generates a fluorescent light image on the basis of a plurality of pieces of first image data and generates a scattered light image on the basis of a plurality of pieces of second image data, and an analysis unit that specifies an area in which there is the sample in the fluorescent light image on the basis of the scattered light image, and sets an analysis area in the fluorescent light image on the basis of the area in which there is the sample.

Abstract: A system for separating biological molecules includes a plurality of capillaries (101), a capillary mount (102), a plurality of optical fibers (145a, 145b), a fiber mount (603), an optical detector (138), and a motion stage (606). The plurality of capillaries (101) are configured to separate biological molecules in a sample. Each capillary (101) comprising a detection portion (121) configured to pass electromagnetic radiation into the capillary (101). The plurality of capillaries (101) are coupled to the capillary mount (102) such that the detection portions (121) are fixedly located relative to one another. Each optical fiber (145) includes a receiving end to receive emissions. The optical fibers (145) are coupled to the fiber mount (603) such that the receiving ends of the optical fibers are fixedly located relative to one another. The optical detector (138) is configured to produce an alignment signal.

Abstract: A microfluidic device and a detection method for the microfluidic device are provided. The microfluidic device includes a driving substrate configured to drive a movement of a droplet; and a position detector configured to detect a position of the droplet on the driving substrate.

Abstract: A test device for testing an oxidation potential of an electrolyte is provided. The test device comprises a cavity, a test unit, a detector, a processing unit, and a display. The test unit comprises a positive plate comprising a first through hole, a negative plate comprising a second through hole, a first infrared window covering the first through hole, a second infrared window covering the second through hole, and an electrolyte located between the positive electrode plate and the negative electrode plate. The first through hole and the second through hole penetrate each other. The first infrared window, the positive plate, the negative plate, and the second infrared window are stacked with each other. An infrared light beam passes through the first infrared window, the first through hole, the electrolyte, the second through hole, and the second infrared window in sequence and then is detected by the detector.

Abstract: The disclosure provides a novel system of storing information using a charged polymer, e.g., DNA, the monomers of which correspond to a machine-readable code, e.g., a binary code, and which can be synthesized and/or read using a novel nanochip device comprising nanopores; novel methods and devices for synthesizing oligonucleotides in a nanochip format; novel methods for synthesizing DNA in the 3? to 5? direction using topoisomerase; novel methods and devices for reading the sequence of a charged polymer, e.g., DNA, by measuring capacitive or impedance variance, e.g., via a change in a resonant frequency response, as the polymer passes through the nanopore; and further provides compounds, compositions, methods and devices useful therein.

Abstract: A multi-channel bio-separation system configured to utilize a cartridge that has a individual, separate integrated reagent (i.e., a separation buffer) reservoir dedicated for each separation channel. The multiple channels may have different characteristics, such as different separation medium of different chemistries, different separation length, different channel sizes and internal coatings. In one embodiment, the cartridge does not include integrated detection optics. Not all channels need to be operative. One or more of the channels in the cartridge may be “dummy channels” that are not operative (e.g., not provided with a capillary tube). A capillary tube may be routed between the reservoir/electrode (anode) of one channel to an electrode (cathode) in another channel, thus allowing a longer length of capillary tube to be used to define a longer separation channel to improve resolution.

Abstract: A molecular scrivener reads data from or writes data to a macromolecule and includes: a pair of shielding electrodes; a scrivener electrode between the first and second shielding electrodes and that electrically floats at a third potential that, in an absence of a charged or dipolar moiety of the macromolecule, is intermediate between the first and second potentials and changes in a presence of the charged or dipolar moiety; a dielectric layer interposed between shielding electrodes and the scrivener electrode; and a nanopore that communicates the macromolecule through the electrodes and dielectric layers. Reading data from or writing data to a macromolecule includes: sequentially receiving, at the scrivener electrode, individual moieties of the macromolecule so that the third potential responds to individual moieties; communicating the macromolecule from the scrivener electrode to the second shielding electrode and from second shielding electrode to expel the macromolecule from the nanopore.

Abstract: A microfluidic apparatus is provided. The microfluidic apparatus includes a base substrate; a microfluidic device on the base substrate and including a plurality of micro fluidic channels; a plurality of light sources of different colors respectively emitting light of different wavelength ranges; a light extraction apparatus for extracting light respectively from the plurality of light sources of different colors and a plurality of photosensors. The light extraction apparatus includes a light guide plate having a plurality of light incident portions for respectively receiving light respectively incident from the plurality of light sources of different colors, the plurality of light incident portions being on a side of the light guide plate away from the microfluidic device; and a plurality of light extractors on the light guide plate.

Abstract: Techniques and apparatus for ion source devices with minimized post-column volumes are described. In one embodiment, for example, an ion source assembly may include a chromatography column in fluid communication with an ion source device, the chromatography column arranged within a minimum distance of the ion source, the minimum distance comprising between about 60 mm and about 150 mm.

Abstract: A microfluidic system is disclosed, including: a first substrate, a second substrate and a droplet flow channel arranged therebetween; a droplet driving unit configured to drive a droplet to move; a first control circuit electrically connected to the droplet driving unit and configured to input a first driving signal to the droplet driving unit to enable the droplet to move along the predetermined movement trajectory; a droplet detection unit configured to detect the droplet and output a detection signal; a second control circuit electrically connected to the droplet detection unit and configured to receive the detection signal and acquire an actual movement trajectory of the droplet; and a signal adjustment unit configured to compare the actual movement trajectory with the predetermined movement trajectory, and if the actual movement trajectory is different from the predetermined movement trajectory, adjust in real time the first driving signal into a second driving signal.

Abstract: An electrophoresis device includes a mounted reference capillary and a focused optical system. A 2D image of light containing autofluorescence and a 2D image of Raman-scattered light are obtained from the separation medium, and pattern data for autofluorescence and Raman-scattered light are obtained from these. An electrophoresis capillary is mounted in the electrophoresis device, pattern data for autofluorescence and Raman-scattered light is obtained in the same way at each of multiple different focal positions of the optical system, and the focal position of the optical system that best matches that of the reference capillary is used as the in-focus position.

Abstract: A detector system is disclosed herein that includes a plurality of light guides. Each light guide is guiding incoming light from a respective object in use, wherein the incoming light is provided by means of an illuminating means. The detector system comprises diffracting means for diffracting the incoming light in different wavelength ranges, at least one focuser for projecting the incoming light exiting the light guides onto the diffracting means, a detector having a detector area for receiving the diffracted light from the plurality of Sight guides, and a control unit. These are arranged to pulsate incoming light via only one light guide at a time based on a pulse timing parameter, and record a spectrum of light diffracted from each light guide and detected by the detector based on the pulse timing parameter.

Abstract: A three-dimensional (3D) forensic evidence system is provided. The system includes a noncontact measurement device operable to measure a distance from the device to a surface. A first camera is operably coupled to the noncontact measurement device, the first camera having a field of view. A light source is operably coupled to the first camera and operable to emit light onto the surface within the field of view. A processor operably is coupled to the first camera, the processor operable to execute computer instructions when executed on the processor for determining 3D coordinates of at least one point in the field of view based at least in part on the distance, and assigning at least one color value to the at least one point in response to determining an interaction of a predefined wavelength of light with a substance in the field of view.

Abstract: A multicolor detection device includes: a condensing lens array 17 in which a plurality of condensing lenses 18, each of which turns light emitted from each of a plurality of light emitting points 1 individually into parallel light beams, are arranged, the light emitting points being arranged in a light emitting point array; at least one spectroscopic element on which the parallel light beams are incident in parallel, the at least one spectroscopic element being common; and at least one sensor on which light beams spectrally separated by the spectroscopic element are incident in parallel, the at least one sensor being common.

Abstract: In one example, an analyte detection package includes a substrate, surface-enhanced luminescence (SEL) structures extending from the substrate and a low wettability housing. The SEL structures have a first wettability for a given liquid. The low wettability housing extends from the substrate to form a chamber between the housing of the substrate about the SEL structures to receive an analyte containing solution. The housing has an inner surface adjacent the chamber, wherein the inner surface has a second wettability for the given liquid less than the first wettability.

Abstract: A micropump includes a body with an inlet and an outlet defined therein. A channel connects the inlet to the outlet. The micropump further includes a magnetic shape memory (MSM) alloy positioned within the channel. The MSM alloy selectively forms a barrier between the inlet and the outlet. The micropump also includes an electrode and/or a transparent window positioned along a surface of the channel. A cavity is selectively formed within a surface of the MSM alloy due to a magnetic field. The cavity is selectively moveable between a first position adjacent to the inlet, a second position adjacent to the electrode and/or the transparent window, and a third position adjacent to the outlet, by altering the magnetic field. By altering a magnetic field applied to the MSM alloy, a fluid may be pumped from the inlet to the electrode and/or the transparent window where the fluid may be analyzed. The fluid may be subsequently pumped to the outlet.

Abstract: A multiple capillary florescent detection system employing optical fiber bundles that each fiber bundle has more than one fiber illuminating each sample vessel.

Abstract: A technique for a nanodevice is provided. A reservoir is separated into two parts by a membrane. A nanopore is formed through the membrane, and the nanopore connects the two parts of the reservoir. The nanopore and the two parts of the reservoir are filled with ionic buffer. The membrane includes a graphene layer and insulating layers. The graphene layer is wired to first and second metal pads to form a graphene transistor in which transistor current flowing through the graphene transistor is modulated by charges or dipoles passing through the nanopore.

Abstract: A sensor for detecting a liquid in a fluid channel of a microfluidic component and to a microfluidic component having such a sensor. The sensor includes an electrode arrangement having a transmitting electrode, a receiving electrode and a first shielding electrode, which are arranged in a coplanar manner on a plane and can be positioned above or below, adjacent to the fluid channel, wherein the transmitting electrode and the receiving electrode are capacitively coupled in that they each have an adjacently arranged edge having a dielectric therebetween.

Abstract: A fiber laser system includes a fiber laser connector having a housing to terminate a fiber that generates a laser beam. A chamber extends internally along a length of the housing. A light trap includes a plurality of threads formed along a wall of the chamber to trap light reflected back to the fiber laser connector in response to an application of the laser beam to a workpiece.

Abstract: A microfluidic device for detecting rare cells in a fluid sample comprises the rare cell and other cells. The microfluidic device comprises an inlet for receiving the fluid sample, a labyrinth channel structure in fluid communication with the inlet, and an outlet in fluid communication with the labyrinth channel structure for collecting the rare cells separated from the other cells in the fluid sample. The labyrinth channel structure comprises at least one channel through which the fluid sample flows. The at least one channel has a plurality of segments and a plurality of corners with each corner defined between adjacent segments. The presence of the plurality of corners induces separation of the rare cells from the other cells in the fluid sample as the rare cells move to a first equilibrium position within the at least one channel when a ratio of inertial lift forces (FZ) and Dean flow (FD) of the fluid sample is from 2 to 10.

Abstract: A method of measuring biological sample properties and a biological sample property measuring apparatus is provided. A method of measuring biological sample properties includes disposing a biomaterial to contact a sensing unit, detecting a radio frequency (RF) signal flowing through the sensing unit, and obtaining an RF property indicator of the biomaterial based on the detected RF signal.

Abstract: An apparatus for discriminating bacteria types using optical scattering patterns is disclosed. The apparatus includes an optical fiber for transferring light emitted from a light source, a lens for controlling a width of the light received from the optical fiber, a linear polarizer for transmitting the light passing through the lens and a bacterial colony, and a capturing unit for capturing an optical scattering pattern of the light transmitted through the linear polarizer.

Abstract: Low-cost and easily-operated microviscometer suitable for medical diagnosis clinical studies and other fluid tests. The equipment consists of a microchannel (2) formed by concatenated microchannels made by micro-manufacturing techniques, and a fluid column position detector inside the microchannel. The microchannels are open at one end and closed at the other end and are made of a single biocompatible material. When a liquid drop is put into the inlet of the microchannel (2), the fluid enters by capillary until the compressed air pressure equals the capillary pressure plus atmospheric pressure. The fluid transient movement from entering the channel until stopping at its balance position is analyzed thus obtaining as a result the viscosity and the capillary pressure of the liquid tested.

Abstract: Disclosed are methods and systems for the simultaneous separation and detection of analytes such as anions and cations in a sample using electrophoresis, the method comprising injecting the sample into an electrophoresis system comprising two separation channels through a single sample injection port which is in fluid communication with the two separation channels, separating analytes such as the cations in a first of the two separation channels and simultaneously separation analytes such as the anions in a second of the two separation channels, and detecting the analytes separated in each of the separation channels.

Abstract: A nanosensor for detecting molecule characteristics includes a membrane having an opening configured to permit a charged carbon nanotube to pass but to block a molecule attached to the carbon nanotube. The opening is filled with an electrolytic solution. An electric field generator is configured to generate an electric field relative to the opening to drive the charged carbon nanotubes through the opening. A sensor circuit is coupled to the electric field generator to sense current changes due to charged carbon nanotubes passing into the opening, and to bias the electric field generator to determine a critical voltage related to a force of separation between the carbon nanotube and the molecule.

Abstract: A single-sided continuous optoelectrowetting (SCOEW) device for manipulating droplets retained in a fluid over the SCOEW device with dynamic patterns of low intensity light, such as from a display screen, is described. A single pair of lateral electrodes are utilized for providing a lateral electric field bias, with transport motion controlled in response to projecting light through a photoconductive layer and dielectric layer adjacent to which droplets are retained. The device is configured for optically manipulating droplets having volumes spanning over five orders of magnitude, and can be configured to perform droplet dispensing, transport, splitting, merging, mixing and other droplet manipulation functions involving any of the above on a single sided surface.

Abstract: The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.

Abstract: A process and a laminating device for producing a microfluidic device are proposed, wherein a film is pressed onto a carrier under the effect of heat and thereby attached to it. During the attachment the film is lifted by the blowing in of gas on the carrier side to prevent it adhering in a non-attachment region and is vented on the remote side. The device is compressed between a holder and a thermode of the laminating device during the lamination. An elastic intermediate layer is arranged in each case between the device and the holder and between the device and the thermode, for evening out the contact pressure and obtaining uniform attachment of the film to the carrier.

Abstract: A microfluidic panel including at least one substrate, one or more channels formed in the substrate, and fluid disposed within the one or more channels. The fluid is selected to store thermal energy and the microfluidic panel is adapted to convert the thermal energy into useable energy or condition the energy to adjust optical wavelength passband of the panel.

Abstract: Systems and methods for air cooling a microfluidic device using confinement channels to isolate cooling air from exposed liquids are disclosed. The systems and methods may also thermally condition the cooling airflow for improved robustness of the microfluidic device. In one embodiment, the air cooling system includes a split-level cooling manifold including an inlet duct that directs cooling air to a microfluidic device and an outlet duct that directs air heated by the microfluidic device away from the microfluidic device. The temperature of cooling air may be measured. The cooling air may be preheated to a temperature that is higher than an expected ambient temperature. The temperature of the cooling air after being heated by a microfluidic device may be measured.

Abstract: A microfluidic chip includes a thin biaxially-oriented polyethylene terephthalate (“BoPET”) film and a micro-channel in the BoPET film. A method for manufacturing a microfluidic chip includes coating UV epoxy on a first side of a BoPET film, placing the BoPET film on a first substrate with the first side facing the first substrate, curing the UV epoxy on the first side of the BoPET film to attach the BoPET film on the first substrate; forming at least one microfluidic pathway in the BoPET film, coating UV epoxy on a first side of a second substrate, placing the second substrate on the BoPET film with the first side of the second substrate facing a second side of the BoPET film, and curing the UV epoxy on the first side of the second substrate to attach the BoPET film to the second substrate. The microfluidic chip may be a multi-layered chip.

Abstract: An optical fiber (500) illuminates the bore (520) of a capillary tube (515) that is used for separating chemicals by capillary electrophoresis (CE). The fiber terminates in either two sloped regions (525) and a curved region (530) or two sloped regions (705) and a flat region (700). Light from these regions is focused on the bore of the capillary tube. Since the fiber is sized to illuminate the core of a CE capillary, it is larger than fibers used in telecommunications and its sloped regions are at angles that would be unsuitable for use in telecommunications. The relatively large diameter of the capillary permits efficient use of a light source (905).