Abstract: Disclosed is an apparatus for denitrifying a solution that includes a denitrification tank configured to receive a portion of the solution for a period of time. Controlling an amount, duration and frequency of introduction of polyethylene glycol into the solution promotes indigenous heterotrophic bacteria depleting dissolved oxygen in the solution and obtaining oxygen from nitrate in the solution.

Abstract: Methods for bioremediation of contaminants in water using soapstock, an acid oil of soapstock, a neutralized acid oil of soapstock or combinations thereof are described. Systems for bioremediation are also disclosed.

Abstract: A waste treatment method includes the concentration of selected strains of bacteria in a selected medium in the presence of nutrients and water, under aerobic conditions. This concentrated batch is thereafter discharged for downstream applications in wastewater remediation. The present invention employs a cultivation chamber having inlet ports and a circular vent port that allows for adequate air introduction and heat release. Aeration is achieved by recirculation of the fluid medium from the top of the apparatus through a pipe that runs the length of the inner wall and is specially configured at the top to minimize cell damage. Fluid can be routed tangentially in both the clockwise and counterclockwise directions within the chamber. The conical bottom also has an orifice allowing for recirculation of the fluid medium tangentially to the sidewalls.

Abstract: The present invention relates to wastewater treatment in general and to methods of controlling odors and degrading compounds contained in wastewater in particular.

Abstract: Provided is a parts washer that includes a multi-tiered basin, a cleaning fluid and a biological component, living within the fluid, that breaks down organic waste. The multi-tiered basin includes a sink member with a false bottom, and a support grid and filter are interposed between the false bottom and a bottom panel of the sink member. The false bottom, support grid, and filter are readily removable from the sink member. The tank is partially filled with the cleaning fluid and a pump and conduit assembly direct a flow of the cleaning fluid to the basin. The cleaning fluid discharged into the basin flows through a drain hole in the false bottom, through the filter and support grid, and then through a drain hole in the bottom panel of the sink member back into the tank for reuse. The cleaning fluid includes, at least, a surfactant that functions to remove organic waste from the parts being washed.

Abstract: A method of prevention of acid rock drainage excludes oxygen from waste rocks that contain ferrous sulfides by building an oxygen bio-barrier over exposed surfaces of the waste rock. The bio-barrier may be formed by applying a slurry to the rock. The slurry may contain several components such as microbial material, one or more nutrients, water or another fluid, and optionally water-absorbent material. The microbial material may include bacteria, algae, fungi or a combination thereof. Additionally, the slurry may contain one or more acid-neutralizing compounds, one or more cellulose-containing substances, and one or more UV protecting agents.

Abstract: Use of a fungal biomass for treating industrial wastewaters containing at least one dye and related method, wherein: i. the fungal biomass contains at least the fungal species Rhizomucor pusillus (Lindt) Schipper; ii. the fungal biomass absorbs the at least one dye, so as to obtain wastewater that is basically free of the at least one dye.

Abstract: The present invention is directed to processes and business methods and wastewater treatment units for converting the waterborne residuals from wastewater generated by food processing plants into an ingredient suitable for use in animal feeds. The ingredient produced by the processes of the present invention has a high protein content and can be used as a replacement for conventional sources of animal feed protein such as fish meal.

Abstract: Methods for increasing the growth and biomass of algae and algae lipids by growing the algae in the presence of a polycationic substance such as soluble or insoluble chitosan and/or chitin are disclosed. Also disclosed are methods of harvesting algae from an aqueous environment by growing the algae in aggregated clumps and/or mats (formed by the inclusion of soluble or insoluble chitosan and/or chitin or other polycationic or cationic substances) that can be easily removed from the aqueous environment either by filtration, surface skimming, and/or growing in a porous containment device, such as a natural or synthetic fabric, and removing the fabric from the aqueous environment containing the aggregated algae. The methods disclosed have direct applications in biofuel and energy production, agricultural feedstock production, nutrient production, greenhouse gas reduction, removal of microconstituents from water, and water reclamation.

Abstract: This invention relates to a method that uses heterothrophic ammonia oxidation bacteria (HAOB) to remove carbon and nitrogen pollutants in wastewater. The method includes the cultivation of the heterotropic bacteria in an activated sludge environment and the removal of carbon and nitrogen from the wastewater. According to the physiological characteristics of HAOB and the principles of combined oxidation of carbon and nitrogen, the method is able to achieve simultaneous removal of carbon and nitrogen under the condition that the cells do not grow. The process is able to be carried out in the temperature range of 6-40° C. No excess sludge is produced in the process. The invention is able to control the process and product composition of anaerobic ammonia oxidation through the control of organic carbon source, and is able to realize zero-accumulation of NO3?N in the nitrification process. The invention can fully utilize existing activated sludge systems to remove carbon and nitrogen.

Abstract: The invention relates to a method for treating effluents containing 2-ethylhexyl nitrate (2-EHN), or nitric ester of 2-ethylhexane-1-ol, in order to reduce at least partly the concentration thereof, characterized in that it comprises growing, in the presence of a suitable substrate, a Corynebacterium urealyticum CIP-I-2126 bacterium and degrading the 2-EHN contained in the effluents by the biomass of said bacterium thus produced. Application: depollution of waters and soils polluted by 2-EHN.

Abstract: Compositions for bioremediation are described. Systems and methods of using the compositions are also disclosed.

Abstract: The present invention relates to wastewater treatment in general and to methods of controlling odors and degrading compounds contained in wastewater in particular.

Abstract: A method for producing a nitrite type nitrification-reactive sludge includes the step of subjecting an activated sludge to alkali treatment so that the pH of the activated sludge containing at least the ammonium oxidizing bacteria and nitrite oxidizing bacteria can be 10 or higher, in order to accumulate ammonium oxidizing bacteria with higher priority in the activated sludge.

Abstract: An aquaculture or wastewater effluent water quality device to improve water quality and food supply in an aquaculture pond or body of water by incubating beneficial microbes in a temperature controlled environment and systematically releasing them to the surrounding water, allowing the beneficial microbes to perform sanitation functions and to help such systems or water bodies overcome the natural production of harmful microbial species without substantially heating or cooling the entire system any more than is required by the present state of the system or body of water or the inhabitants thereof.

Abstract: A biomethod using a consortium of Pseudomonas sp. to reduce contaminants such as 1,4-dioxane in groundwater.

Abstract: The present invention relates to wastewater treatment in general and to methods of controlling odors and degrading compounds contained in wastewater in particular.

Abstract: A composition including the fermentation supernatant from a fermentation of yeast is intended to be conveniently introduced through the wastewater plumbing system of a private home or other facility into a septic system servicing the home or other facility to substantially accelerate the ability of the bacteria resident in the septic system to substantially digest biologically available organic compounds present in the septic system, and methods of accomplishing the same.

Abstract: A lagoon of effluent is stablished, comprising a set of sub-portions at different levels in the lagoon associated with a corresponding set of concentrations of dissolved oxygen, each sub-portion of the lagoon having a different concentration of dissolved oxygen. An incubator comprising a set of sub-portions associated with said set of concentrations of dissolved oxygen is established, each sub-portion of the incubator having a different concentration of dissolved oxygen. A volume of effluent from each sub-portion of the lagoon is transmitted to a corresponding sub-portion of an incubator having a substantially similar concentration of dissolved oxygen as said sub-portion of the lagoon. A first remediated volume of effluent is generated in said corresponding sub-portion of the incubator responsive to proliferating a first microorganism which uses a first compound in said volume of effluent as substrate for growth, the first microorganism enabling a first chemical reaction which alters the first compound.

Abstract: A method for degrading, detackifying and reducing solvent in water comprising organic solvent-laden water-borne paint that comprises adding to the water an effective degrading, detackifying and/or solvent-reducing amount of at least one microorganism culture and sufficient micronutrients to sustain the growth of the at least one microorgansim culture and to reduce solvent content of the water. A method of reducing chemical oxygen demand in water comprising organic solvent-laden water-borne paint, wherein the water contains an excess amount of organic solvent from one or both of paint spray operations and paint spray nozzle cleaning operations, the method comprising adding to the water an effective degrading and detackifying amount of at least one microorganism culture and micronutrients to sustain the growth of the at least one microorgansim culture, whereby chemical oxygen demand in the water is reduced by at least 50% relative to the same system without adding the micronutrients.

Abstract: A bioremediation method for accelerated biological degradation of petroleum hydrocarbons in a sea ice-covered polar region comprising contacting the petroleum hydrocarbons with an inoculum. The inoculum includes a bacterial mixture, nutrients, and an environmentally friendly carrier material. The bacterial mixture includes a plurality of cold-adapted autochthonous bacterial strains, wherein each of the bacterial strains are active at an ambient temperature of ?3° C., and wherein each of the bacterial strains has a different temperature tolerance range, a different salinity tolerance range, a different degradation spectrum, and a different potential to emulsify oil. Bacteria of the bacterial strains are immobilized on the environmentally friendly carrier material.

Abstract: A composition including the fermentation supernatant from a fermentation of yeast is intended to be conveniently introduced through the wastewater plumbing system of a private home or other facility into a septic system servicing the home or other facility to substantially accelerate the ability of the bacteria resident in the septic system to substantially digest biologically available organic compounds present in the septic system, and methods of accomplishing the same.

Abstract: The present invention provides a mixed bacterial culture, designated as the culture Atz Mix 1, which degrades atrazine at various temperatures (10° C. to 30° C.) in a wide range of atrazine concentrations (several ppb to ten thousand ppm), without formation of toxic metabolites. Atz Mix 1 is a stable mixed culture and includes catabolic genes trzN, atzB, atzC coding the enzymes for the degradation of atrazine to cyanuric acid, and the gene trzD coding the enzymes for subsequent opening of s-triazine ring. The invention further includes a microbiological method of degrading atrazine and other s-triazine compounds for remediation of atrazine-contaminated soils, even those rich in nitrogen, as well as for accelerating the process of atrazine mineralization in waste waters containing high concentrations of s-triazine compounds.

Abstract: Use of a fungal biomass for treating industrial wastewaters containing at least one dye, wherein: i. the fungal biomass contains at least the fungal species Rhizopus stolonifer (Ehrenberg) Vuillemin; ii. the fungal biomass absorbs the at least one dye, so as to obtain wastewater that is basically free of the at least one dye.

Abstract: The present invention provides a method and gaseous composition for the in situ bioremediation of soil and groundwater contaminated with organic compounds, including halogenated hydrocarbons. The gaseous composition, which readily permeates a subsurface region, comprises hydrogen (H2) and one or more volatile phosphates, such as triethylphosphate (TEP) and tributylphosphate (TBP). The volatile phosphates serve as nutrients that stimulate the growth and activity of indigenous microbes that are capable of degrading the contaminants. The addition of hydrogen facilitates the direct reductive dehalogenation of highly halogenated contaminants. The gaseous composition may optionally contain one or more of a volatile alkane and nitrous oxide as additional supports for microbial growth, and carbon dioxide to lower the pH of remediation sites that are highly alkaline.

Abstract: A mixed culture of Dehalococcoides species is provided that has an ability to catalyze the complete dechlorination of polychlorinated ethenes such as PCE, TCE, cDCE, 1,1-DCE and vinyl chloride as well as halogenated ethanes such as 1,2-DCA and EDB. The mixed culture demonstrates the ability to achieve dechlorination even in the presence of high source concentrations of chlorinated ethenes.

Abstract: A synergistic composition is provided for controlling odor from waste products. The composition comprises a combination of nitrate salt, sulfide-consuming compound, pH-elevating compound, sulfide-oxidizing, nitrate-reducing bacteria, and sulfide-oxidizing enzyme. The method includes adding a sufficient amount of the composition to a waste stream to provide sufficient sulfide-consuming compound to effect immediate removal of sulfide. The composition incorporates a pH elevating compound, which both decreases the amount of gaseous H2S and puts the aqueous phase into a pH range where naturally occurring bacteria can more easily metabolize the sulfide. The composition also includes one or more nitrate salts which will accomplish longer term prevention of odors. Specific bacteria are incorporated into the formulation to insure that the nitrate has the right type and amount of bacteria present to prevent formation of and/or consume sulfide.

Abstract: Disclosed is a method of denitrifying a solution including introducing into the solution an amount of a carbon source within a duration and at a frequency so that indigenous heterotrophic bacteria deplete dissolved oxygen in the solution and obtain oxygen from nitrate in the solution. Related apparatuses and compounds also are disclosed.

Abstract: A biological removal method of phosphorus and nitrogen includes supplying a mixture gas into a gas holder. Inflow water and return water are supplied into the gas holder to make the mixture gas dissolved in the inflow water and the return water. The return water was provided from a granulation biological reaction tank. The inflow water and the return water including the dissolved mixture gas are transported to the granulation biological reaction tank. A nitrogen-based component is removed from the inflow water and the return water using methan-oxidizing bacteria granulated in the granulation biological reaction tank. Treated water without the nitrogen-based component is transported to an anoxic tank for treatment, and discharged from the anoxic tank.

Abstract: A method for reducing a production of a build-up slime in a papermaking process is provided. The method includes steps of adding a dispersing agent into the papermaking material to obtain a first mixture, mixing and culturing the first mixture, adding an antagonist into the first mixture to obtain a second mixture, mixing and culturing the second mixture for reducing the production of the slime, and re-adding the antagonist into the mixed and cultured second mixture after a specific time period for further reducing the production of the slime.

Abstract: A method of biological treatment of organic wastewater includes a biological treatment step for introducing organic wastewater to a biological treatment tank and biologically treating the organic wastewater; and a solid-liquid separation step for separating biologically treated liquid in the biological treatment tank into sludge and treated water. The method further includes a sludge treatment step for introducing a portion of the sludge in the biological treatment tank and/or at least a portion of the sludge separated in the solid-liquid separation step into a sludge treatment tank having a submerged filter element with a pore diameter of 5 to 100 ?m in the tank and subjecting the sludge to aerobic biological treatment; and a permeated liquid-returning step for returning the liquid permeated through the submerged filter element to the biological treatment tank.

Abstract: Remediation for groundwater, soil, sediment bodies, bodies of water, tanks, pipes, and their surrounding areas are described. Remediation includes treating a site contaminated with toxic organic compounds by assessing for the presence or absence of suitable ozonophilic bacteria at the site and introducing ozone to the site. The ozonophilic bacteria are introduced to the site if the ozonophilic bacteria are not present or not present in sufficient quantities in the site. Ozone is introduced to the site in an amount sufficient to stimulate growth of the suitable ozonophilic bacteria. Other oxidants can also be used.

Abstract: The present invention provides a mixed bacterial culture, designated as the culture Atz Mix 1, which degrades atrazine at various temperatures (10° C. to 30° C.) in a wide range of atrazine concentrations (several ppb to ten thousand ppm), without formation of toxic metabolites. Atz Mix 1 is a stable mixed culture and includes catabolic genes trzN, atzB, atzC coding the enzymes for the degradation of atrazine to cyanuric acid, and the gene trzD coding the enzymes for subsequent opening of s-triazine ring. The invention further includes a microbiological method of degrading atrazine and other s-triazine compounds for remediation of atrazine-contaminated soils, even those rich in nitrogen, as well as for accelerating the process of atrazine mineralization in waste waters containing high concentrations of s-triazine compounds.

Abstract: A method of treatment of sewage comprising adding at least one cell signalling chemical (CSC) to a sewage substrate, wherein the at least one CSC regulates activity of a microbial population in the sewage substrate. In particular, methods of reducing odor in sewage treatment systems, preventing corrosion in sewage treatment systems, enhancing microbial digestion of sewage, managing methane gas production in a sewage treatment system, enhancing digestion of sewage sludge, resuscitating dormant microbes or microbes that are in a starvation or stationary phase in a sewage substrate and controlling the bacteria responsible for oxidation or reduction of nitrogenous compounds in a sewage substrate, are provided.

Abstract: Disclosed is a method for the anaerobic microbial dechlorination of polychlorinated biphenyls (PCBs). Polyhalogenated ethenes are used to stimulate the growth and dechlorinating activity of PCB dechlorinating bacteria in a contaminated soil or sediment. This method may be further coupled with aerobic microbial degradation of PCBs for the complete destruction of PCBs in soils and sediments. Polyhalogenated ethenes are also used in growth media for the culturing of PCB dechlorinating bacteria.

Abstract: There is a biological composition and method of use. Included is a microorganism quantity, and a nutrient quantity which may have a supplement, a short-term nutrient and a long term nutrient. The composition may be applied to a container having water wherein the microorganisms rapidly reproduce. As the microorganisms reproduce they may be extracted. Once extracted, they may be introduced into a cleaning system and dispersed to surfaces of an animal production facility. There, the microorganisms may process animal waste and the slime associated thereto. Further, the microorganisms may flow into a lagoon to beneficially participate in waste processing. Still further, the microorganisms may enhance the ability of the lagoon to prepare the waste for distribution to plants.

Abstract: The present invention utilizes fungal spore mass or hyphal fragments in burlap bags or sacks filled with biodegradable materials. The fungi may include saprophytic fungi, including gourmet and medicinal mushrooms, mycorrhizal fungi, entomopathogenic fungi, parasitic fungi and fungi imperfecti. The fungi function as keystone species, delivering benefits to both the microsphere and biosphere. Such fungal delivery systems are useful for purposes including ecological rehabilitation and restoration, preservation and improvement of habitats, bioremediation of toxic wastes and polluted sites, filtration of agricultural, mine and urban runoff, improvement of agricultural yields and control of biological organisms.

Abstract: A new and improved method for treating mine influenced water (MIW) or other environmental contamination is disclosed. A number of electron donors are disclosed, including chitin complex-containing materials. The chitin complex-containing materials may modify the pH and reductive-oxidation potential of the contaminated media within the subsurface or within a treatment cell to facilitate metal removal through biological, chemical, and physical means, or a combination thereof. Bioremediation enhancing agents such as yeast products may also be used to facilitate microbial treatment of the contaminated sources. The yeast products may work with microbes to improve the rate of contaminant removal and promote microbial growth.

Abstract: A 10,000 gallon sequencing batch reactor tank for the on-site bio-degradation of oily sludge. Bacteria already present in and adapted to oily sludge degrade the hydrocarbons found in oily sludge within two weeks from 20,000 ppm to less than 100 ppm. A degradation cycle requires 5 days. After five days a recirculation pump and aeration system are turned off and solids are allowed to settle to the bottom of the tank. An ultrafiltration unit connected to the tank requires approximately 16 hours to process the contents of the reactor tank.

Abstract: The present invention relates to wastewater treatment in general and to methods of controlling odors and degrading compounds contained in wastewater in particular.

Abstract: The present invention is directed to a substantially odorless biologically mediated treatment process for solid and liquid organic wastes. The present invention also provides for a novel nutrient rich humus material produced from the biologically mediated treatment process. The bioconversion process of the present invention results from low electron acceptor concentrations and high quantities of microorganisms in a diverse microbial community.

Abstract: The invention discloses a biotechnological process for the treatment of hydroxide sludge containing As produced in potable water production processes which use FeCI3 as a coagulant. The process seeks to modify the physicochemical properties of the produced sludge in such a way to facilitate the use of mechanical dehydration methods that allow decreasing the sludge volume and facilitating its final disposal.

Abstract: The invention concerns an isolated or purified polypeptide having an activity in the MTBE degradation path, and/or at least one of the catabolic intermediates of MTBE, preferably selected from the group consisting of tert-butyl alcohol (TBA), 2-methyl 1,2-propanediol (2-M1, 2-PD), hydroxyisobutyraldehyde, hydroxyiso-butyric acid (HIBA), said polypeptide being selected from the group consisting of: a) a polypeptide comprising a sequence of amino acids selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 or SEQ ID NO:10; b) a polypeptide comprising a sequence of amino acids having at least 70% identity, preferably 75%, 80%, 90%, 95%, 98% or 99% with the sequence of amino acids of a polypeptide as defined in a); a polypeptide as defined in a) or b) whereof the sequence of amino acids comprises a substitution, deletion, insertion, addition or mutation of one or several amino acids over its entire length.

Abstract: Liquid chemical compositions are disclosed for anaerobic biodegradation, detoxification, and transformation of toxic organic and inorganic compounds in a contaminated geologic media under reducing conditions, including, but not limited to, denitrifying, manganese-reducing, iron-reducing and sulfate-reducing conditions. One such liquid chemical composition includes sodium nitrate in the range of one-fifth (0.2) to four (4) pounds per gallon of the chemical composition; sodium hexametaphosphate or other biologically hydrolyzable ring or linear polyphosphate in the range of one twentieth (0.05) to five (5) pounds per gallon of the chemical composition; a surfactant in the range of 0.01% to 10% by volume of the chemical composition; and a diluent in the form of water.

Abstract: A method and apparatus for remediating a failing wastewater treatment system comprising a positive air, oxygen, ozone, or combination thereof, generating pressure pump (40) directing the air, oxygen, ozone or combination through a tube (50) to an air stone (60) suspended in the effluent. Attached growth bacteria grow on a plurality or random directional brushes (116) in an effluent tank, e.g., septic tank (14).

Abstract: A method for degrading, detackifying and reducing solvent in water comprising organic solvent-laden water-borne paint that comprises adding to the water an effective degrading, detackifying and/or solvent-reducing amount of at least one microorganism culture and sufficient micronutrients to sustain the growth of the at least one microorgansim culture and to reduce solvent content of the water. A method of reducing chemical oxygen demand in water comprising organic solvent-laden water-borne paint, wherein the water contains an excess amount of organic solvent from one or both of paint spray operations and paint spray nozzle cleaning operations, the method comprising adding to the water an effective degrading and detackifying amount of at least one microorganism culture and micronutrients to sustain the growth of the at least one microorgansim culture, whereby chemical oxygen demand in the water is reduced by at least 50% relative to the same system without adding the micronutrients.

Abstract: Improved systems and methods for removing the load of perchlorate and other oxyanions off of ion exchange resins through bioregeneration are disclosed. The improved systems and methods rely upon direct contact between the resin and a liquid product containing enrichment cultures of or pure strains of perchlorate-destroying microorganisms. Controlled temperatures also lead to improvements as do upflow conditions leading to bed expansion. These methods can be incorporated into methods for removing perchlorate from aqueous streams.

Abstract: Systems and methods for co-enhancing contaminant degradation such as LNAPL and DNAPL degradation. Embodiments of the inventive technology may relate to assuring adequate amounts (e.g., concentrations) of certain substances (e.g., nitrogen, electron acceptor, bacteria, microbial substrate) so that degradation of LNAPL and DNAPL is enhanced as intended. Any of the inventive methods may involve the amendment of groundwater with one or more particular substance (uric acid, electron acceptors, microbial substrate, as but a few examples) in order to enhance DNAPL, and perhaps also, LNAPL degradation.

Abstract: Use of a fungal biomass for treating industrial wastewaters containing at least one dye and related method, wherein: i. the fungal biomass contains at least the fungal species Rhizomucor pusillus (Lindt) Schipper; ii. the fungal biomass absorbs the at least one dye, so as to obtain wastewater that is basically free of the at least one dye.

Abstract: A method and system for forming an in situ subsurface filter in a contaminated aquifer and for removing contaminants from groundwater drawn from a contaminated aquifer. The filter is produced in situ by injecting an aqueous solution comprising bacteria-stimulating chemical components through a well to bioactively generate a subsurface mineral filtering zone surrounding the well. As water is subsequently drawn through the well, contaminants are removed from the water as it passes through the filtering zone.