Abstract: A separating agent which combines a high optical resolving power inherent in polysaccharide derivatives with sufficient solvent resistance and a method with which the separating agent can be produced efficiently in short steps. The separating agent for enantiomeric isomers is characterized in that a polymerizable polysaccharide derivative of a polysaccharide derivative having polymerizable functional groups and a polymerizable monomer having polymerizable unsaturated groups are copolymerized with a carrier having polymerizable functional groups to be chemically bound mutually. The separating agent is preferably used for high performance liquid chromatography (HPLC) and has an excellent solvent resistance.

Abstract: The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.

Abstract: The invention relates to a method of preparing multi-modal anion-exchange ligands, which comprises providing a cyclic three-functional scaffold comprising an amine, a carbonyl and a thiol; optionally derivatisation of the amine of the scaffold to provide an anion-exchanging group and/or to protect the amine; and aminolysis to open up the derivative by adding a reagent comprising an amine coupled to a residue R in order to add said amine to the carbonyl carbon of the opened scaffold. The scaffold is advantageously homocysteine thiolactone. In one embodiment, the method comprises an additional step of immobilising the opened scaffold to a base matrix to provide a separation medium, such as a chromatography medium.

Abstract: Sol-gel derived monolithic silica columns containing entrapped dihydrofolate reductase were used for frontal affinity chromatography of small molecule mixtures. The output from the column combined with a second stream containing the matrix molecule (HCCA) and was directly deposited onto a conventional MALDI plate that moved relative to the column via a computer controlled x-y stage, creating a semi-permanent record of the FAC run. The use of MALDI MS allowed for a decoupling of the FAC and MS methods allowing significantly higher ionic strength buffers to be used for FAC studies, which allowed for better retention of protein activity over multiple runs.

Abstract: Onium salt chemistry can be used to deposit very uniform thickness stationary phases on the wall of a gas chromatography column. In particular, the stationary phase can be bonded to non-silicon based columns, especially microfabricated metal columns. Non-silicon microfabricated columns may be manufactured and processed at a fraction of the cost of silicon-based columns. In addition, the method can be used to phase-coat conventional capillary columns or silicon-based microfabricated columns.

Abstract: Polymeric surfactants (molecular micelles) are disclosed for use in open tubular capillary electrochromatography or in high performance liquid chromatography. For example, fused silica capillaries are coated with thin films of charged polymers in a polyelectrolyte multilayer (PEM). A PEM coating may be formed in situ by alternate rinses with positive and negative polyelectrolytes. At least the innermost of the negatively charged polymer layers is a molecular micelle. Prototype embodiments have successfully separated seven benzodiazepines from one another. The run-to-run, day-to-day, week-to-week and capillary-to-capillary reproducibilities were very good, with relative standard deviation values less than 0.01. The PEM-coated capillary was very robust over at least 200 runs. Stability against high and low pH values was also observed. Using chiral polymerized micelles, chiral separations may be achieved, as was demonstrated with a separation of the enantiomers of 1,1?-binaphthyl-2,2?-dihydrogenphosphate.

Abstract: According to the process for producing amino acid or salt thereof in the present invention, in the adsorption step, an amino acid-containing aqueous solution is fed into a pressure tight column so that a free amino acid is adsorbed on a carbonate-type anion exchange resin packed in the pressure tight column. Subsequently, in the elution step, eluent liquid containing a hydrogen carbonate ion and/or a carbonate ion is injected into the pressure tight column in a pressurized state to elute the amino acid adsorbed on the anion exchange resin and simultaneously to regenarate the anion exchange resin into the carbonate-type. In the case of purifying an acidic amino acid, an aqueous ammonium carbonate solution is employed as the eluent liquid. In the case of purifying a neutral amino acid, an aqueous carbonic acid solution, an aqueous hydrogen carbonate solution, an aqueous ammonium hydrogen carbonate solution or an aqueous ammonium carbonate solution is employed as the eluent liquid.

Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

Abstract: A polymer modified porous substrate for solid phase extraction or chromatography, comprising a porous substrate and a polymeric monolith formed thereon, wherein the polymeric monolith comprises C5-10 monocyclic or bicyclic aryl or heteroaryl, optionally substituted with -L-Qp-Rq; where q is 0-3, p is 0-5, Q is —NRC(O)—, —C(O)NR—, —OC(O)NR—, —OC(O)R—, —NRC(O)O—, —NRC(O)NR—, —NCO, —CHOHCHOH—, CH2OCHCH2O—, —(CH2CH2O)s—, —(CH2CH2CH2O)s—, —C(O)—, —C(O)O—, —CH2C(O)CH2—, —S—, —SS—, —CHOH—, —O—, —SO—, —SO2—, —SO3—, —OSO3, —SO2NR—, —NRq—, and —NRq+—, —CN, —NC, —CHOCH—, —NHC(NH)NH—, —NO2, —NO, —OPO3—, —OH; and R is hydrogen, C5-10 monocyclic or bicyclic aryl or heteroaryl, C1-12 branched, unbranched, or cyclic hydrocarbyl; L is a bond or a C1-12 branched, unbranched, or cyclic hydrocarbyl. Methods of preparing and using the polymer modified porous substrates are disclosed.

Abstract: The present invention provides a method having a high productivity for preparative separation by simulated moving bed chromatography. That is, in a filler for enantiomeric isomer separation by simulated moving bed chromatography, which has a porous carrier carrying a polysaccharide derivative thereon and has a TS coefficient in a range of 0.55 to 1.20, the TS coefficient being defined by the below-shown formula (I), and obtained by using an enantiomeric isomer separation column for simulated moving bed chromatography in which the filler is filled in a column tube by a slurry filling method: TS coefficient=[Vc?[t(TS)?t(blank)]×FR]/[t(TS)?t(blank)]×FR,??(I) wherein Vc (cm3): a column volume; FR (ml/min.): a flow velocity; t(TS) (min.): an elution time for tetrakis(trimethylsilyl)silane (=TS); and t(blank) (min.): an elution time for TS in the state where the column is not connected.

Abstract: A flow-through ion exchange medium comprising a monolithic stationary phase having interconnecting pores defined by pore walls, and fine ion exchange polymeric layering particles irreversibly bound directly or indirectly to the pore walls, and methods of making such medium and its use on chromatographic separators.

Abstract: A separation element is provided for use in a chromatographic analysis system for the separation of a mixture using a stationary phase and a mobile phase. The separation element comprises a fluid transmissive fiber rod comprising a plurality of fibers entangled to provide a tortuous path through which a mobile phase may flow as part of a separation process. The plurality of fibers provides a stationary phase for the separation process.

Abstract: There is described a chromatographic separation member including a substrate carrying on at least one surface a thin layer of a chromatographically active capture material. Also described is a chromatographic separation method for separating a component of interest from a fluid utilizing the chromatographic separation member.

Abstract: Ultrasound transceivers (20) are distributed up the side wall of a chromatography column (1) and enable tracking of the progress of a rising bed front (5) during packing. Comparison with a predetermined stored profile is used for feedback control of the packing pump P. Other uses of ultrasound monitoring of the column interior are disclosed.

Abstract: This invention relates to a pipette tip designed to perform filtration or sample separation on the basis of one or more perforations, holes or incisions performed on the tip. Such perforation, holes or incisions are large enough to permit the passage of fluids or smaller particles, but small enough to block the passage of solid materials or larger particles, such as those including chromatography media. Such a pipette tip can thus be used for the filtration or separation of samples without the use of a filter, secondary solid matrix or any other components needed to hold chromatography media and other solid matrices within the pipette tip during the separation process.

Abstract: A liquid sample (104) introduced in a main flow passage (101) is held in a dam portion (105), and a trigger liquid (106) is filled in a trigger flow passage (102). In this state, the trigger liquid (106) is further introduced at desired timing into the trigger flow passage (102) so that the front end portion of the level of the trigger liquid (106) is advanced and the front end portion is brought to be into contact with the dam portion (105). This causes the liquid sample (104) to move to the right (downstream side) in the figure, resulting in the liquid sample (104) flowing out to the downstream side of the main flow passage (101). This means that the trigger liquid (106) provides priming to realize a liquid switch.

Abstract: Monolithic cartridges including a plurality of nominally aligned polymer fibers can be used as stationary phase materials for liquid chromatography separations. Bundles of fibers are packed together so as to form capillary channels between the fibers. Different polymer compositions permit the “chemical tuning” of the separation process. The fibers can be physically or chemically bonded at spaced locations throughout the cartridge or can be packed together under pressure by use of an encasing wrap to form the capillary channels. Use of fibers allows a wide range of liquid flow rates with very low backpressures. Applications in HPLC, cap-LC, prep-scale separations, analytical separations, waste remediation/immobilization, extraction of selected organic molecules/ions from solution, purification of liquid streams (process waste, drinking water, pure solvents), selective extraction of cell matter and bacteria from growth media, and immobilization of cell matter and bacteria are envisioned.

Abstract: The present invention provides a production method with which a porous carrier can be allowed to evenly carry a polysaccharide derivative. That is, the present invention provides a method of producing an enantiomeric isomer-separating filler, including bringing a porous carrier and a solution of a polysaccharide derivative into contact with each other through a stirring operation in a stirring device, to allow the porous carrier to carry an optically active polymer compound, in which: a two-axis vertical stirring device is used as the stirring device; and the porous carrier is allowed to carry the polysaccharide derivative in a carrying amount of 23 mass % or more.

Abstract: Embodiments of the present invention are directed to porous resins for solid phase extractions. The resins feature at least one hydrophobic component, at least one hydrophilic component and at least one ion exchange functional group. The resins exhibit superior wetting and ion exchange performance.

Abstract: In an extensive Matched Ion Polynucleotide Chromatography (MIPC) system and method, and the computer programs or software associated therewith, the system provides automated options for sample selection, mobile phase gradient selection and control, column and mobile phase temperature control, and fragment collection for a wide variety of MIPC separation processes. MIPC separation processes can be applied to effect size-based separation of DNA fragments, mutation detection, DNA fragment purification, PCR process monitoring and other novel processes. This invention is directed to the system and software which automates many of these procedures, facilitating use of the system to achieve complex separation methods. In one embodiment of the invention, a user specifies a size range of double stranded DNA fragment(s) in a mixture, the software calculates a solvent gradient to elute the fragment(s), and the system performs the chromatographic separation using the calculated gradient.

Abstract: The present invention provides a separating agent for enantiomeric isomers having no large UV absorption, as represented by an aromatic group, and having high asymmetric recognizability, particularly, high asymmetric recognizability in an aqueous mobile phase, or a separating agent for enantiomeric isomers containing a polysaccharide alicyclic ester derivative free from an aromatic group as an effective component.

Abstract: Provided is a method of purifying a macrolide, especially tacrolimus, that includes loading macrolide onto a bed of sorption resin and elting with a suitable eluent such as a combination of water and tetrahydrofuran.

Abstract: Provided are methods of separating an immunoreactive compound from at least one immaterial component, using a simulated moving bed (“SMB”) system and a SMB apparatus for use in these methods. Also provided are purified immunoreactive compounds prepared using the SMB methods and apparatus and methods of treatment with the purified immunoreactive compounds.

Abstract: A method for separation of actinide elements comprising feeding a solution containing actinide elements such as americium, curium, californium and the like, into a resin column in which a weakly basic primary, secondary or tertiary anion exchange resin obtained by resinifying pyridine, imidazole or alkylamine has been packed, and then feeding an eluent of a mixed solution of nitric acid and alkyl alcohol such as methanol, ethanol, propanol and the like into the resin column to chromatographically separate the actinide elements from each other. This method makes it possible to efficiently separate the actinide elements from each other by a unit operation at ordinary temperature and ordinary pressure while avoiding oxidation operation, and hence makes it possible to avoid generation of secondary wastes and operations difficult in terms of engineering, such as precipitation.

Abstract: The present invention refers to a method of separating casein from animal milk, especially, after defatting the animal milk as the pretreatment, the processing of the defatted milk aforesaid through a ceramic filtration membrane to remove the casein in the milk. The pretreatment process of separating the casein from the milk is then complemented by other processes of purification, so as to obtain the target protein of higher purity.

Abstract: A separation column for chromatography or a medium for solid phase extraction, which comprises a hollow capillary and a collected body packed in the hollow capillary and serving as a stationary phase, the collected body comprising long fibers having an adsorbing ability selective towards a target solute and arranged in the axial direction of the capillary, and a sample injection system which comprises the foregoing hollow capillary incorporated into a loop of a valve or a passage connecting two valves, the valve(s) being used as an injector for the chromatography. The use of the polymeric long fibers, which have chemical structures specially designed so that they can specifically interact with a target substance (a solute or an analyte) to be analyzed, permits the selective or preferential extraction and/or concentration of the solute.

Abstract: The present invention relates in general to the preparation and use of matrices having solid spaces, interstitial spaces and interstitial polymer networks. In particular, the interstitial polymer networks have utility in chemical and biochemical separations, solid phase synthesis, catalysis of chemical reactions, and immobilized enzyme reactors. The interstitial polymer networks in one embodiment comprise crosslinked polymers suspended in the interstitial spaces from and/or between solid particles. The matrices are characterized by high ligand and functional group density and by reversible high sorptive and binding capacity, and are substantially accompanied by a very low nonspecific adsorption or interaction with molecules such as proteins. Moreover, the matrices of the invention exhibit other characteristics highly desirable in chromatographic and catalytic applications, such as high physical rigidity, high ligand density, chemical stability, high ligand reactivity, and rapid exchange and reaction kinetics.

Abstract: Granulated products are provided and include carbonaceous particles and a carbonized agent or binder. The agent or binder is preferably a synthetic resin, pitch component, or mixture thereof. Packing materials for packing columns used in chromatographic separations are also provided as are methods of chromatographic separation using the materials. In addition, methods are provided to provide a variety of different types of carbonaceous products. A variety of chemical groups can be, prior to heat-treatment and/or thereafter, attached to the granules to form modified granules.

Abstract: The application describes apparatus and methods using ultrasound transmitted through a chromatography column bed to determine the status of materials in the bed space, particularly during chromatography runs. Detected conditions include the profile of a band of eluting material—to see whether it is satisfactory—the arrival of a band of material at the column output, the fitness for use of the ed packing, the passage of different mobile phases, e.g. salt fronts, and other conditions. Aspects claimed include control processors and corresponding programmed products.

Abstract: A solid phase extraction (SPE) device having a reservoir with an opening; a well comprising an internally tapered wall, the well having a wider interior diameter at an end closest to the opening than at an exit spout; a first filter within the well; a bed of sorbent particles within the well below the first filter; and a second filter having a diameter smaller than the first filter within the well below the bed of sorbent particles and above the exit spout is provided. A method of performing SPE using the device is also provided.

Abstract: A method for separating one or more components of a biomolecule mixture by means of an ion exchange chromatographic system operated in the displacement mode includes sequentially perfusing the system with a first solution including the biomolecule mixture, and a second solution including a displacer having a structure selected from formula I and formula II:

Abstract: The invention encompasses an improved, rapid, size-exclusive method for screening for small molecular weight ligands that bind specifically to a protein target, using size-exclusion separation, ultrafiltration, and mass spectrometry.

Abstract: The present invention relates to the preparation of a metal chelating separation medium, which comprises providing a compound defined by formula: R—O—CO—(CH2)n—CHNH2—CO—OH wherein R is a selectively cleavable group; and n is an integer of 1–4; reacting it with ethyl alcohol; derivatisation; cleavage of R; immobilisation of the resulting compound to a base matrix; and deprotection of the carboxylic acid. In one embodiment, the compound provided in the first step is ethyl ester of aspartic acid or of glutamic acid, and the derivatisation agent is bromo-acetic acid ethyl acetate.

Abstract: This invention teaches a purification technology to extract an organic solution of paclitaxel by using a “Load and Lock Axial Compression Column” for industrial scale preparative high performance liquid chromatography. The “Load and Lock Axial Compression Column” is one in which a piston is used to pack and unpack the chromatography bed substantially to avoid voids therein, and to maintain bed compression during use. This effectively substantially prevents the formation of voids in the bed. The column can be packed with any packing material, including small particle size (e.g., about 10 ?m) media, and very high plate numbers are generated. The bed length can be adjusted by controlling the amount of packing material used to prepare the column.

Abstract: Methods and devices based on capillary monolithic columns, preferably consisting of an underivatized poly(styrene-divinylbenzene) monolith, for separating a mixture of polynucleotides by ion pair-reverse phase-high performance chromatography (IP-RP-HPLC).

Abstract: The invention refers to a new process for the production of purification of high purity plasmid DNA. The process comprehends: a) the production of cells containing plasmid DNA, b) the disruption of the cells in order to obtain a lysate containing plasmid DNA, c) a concentration step by precipitation with isopropanol, d) a pre-purification and conditioning step by the addition of ammonium sulphate, e) a purification step using hydrophobic interaction chromatography, f) a final concentration and/or buffer exchange step. The process is scaleable, it does not use enzymes or mutagenic agents and it enables the preparation of plasmid DNA with pharmaceutical grade, which complies with the requirements of regulatory agencies. The invention belongs to the technical domain of Biochemical Engineering/Biotechnology.

Abstract: A multicapillary column especially useful for liquid chromatography and sample preparation comprising a plurality of uniform capillaries coated with an insoluble stationary phase, wherein the thickness of the stationary phase is correlated with the radius of the individual capillaries for high efficiency.

Abstract: The present invention relates to a solid and method useful in separating chemical components in a complex mixture when at least one of the chemical components of the mixture is capable of being selectively adsorbed. The solid of the present invention comprises an inorganic substance and moieties (R10) located on at least one surface of the inorganic substance, wherein said inorganic substance is an inorganic oxide and the surface moiety is selected from the group consisting of —CH2OH, —CH(OH)2, —CH(OH)CH3, —CH2CH2OH, —C(OH)2CH3, —CH2CH(OH)2 and —CH(OH)CH2(OH). Binding moiety, optionally attached to the inorganic substance via a linker, can also be located on the surface of the solid.

Abstract: An affinity matrix for use in affinity based molecular pull down and immunoprecipitation procedures. The affinity matrix comprises a polymeric support, a dye attached to a fraction of the polymeric support to enable optical detection of the polymeric support, and an affinity ligand other than the dye attached to a fraction of the polymeric support for the capture of a molecule. Also provided is a method for the isolation of a biomolecule from an aqueous solution. The method comprises combining the aqueous solution with an affinity matrix comprising a polymeric support and separating the affinity matrix from the aqueous solution. A dye is attached to a fraction of the polymeric support which enables optical detection and monitoring of the affinity matrix and, accordingly, reduces the likelihood of the loss of affinity matrix during the separation step. In addition, an affinity ligand other than the dye is also attached to a fraction of the polymeric support for the capture of the biomolecule.

Abstract: An eluent for ion chromatography for analyzing divalent cations, which comprises an acid and at least one amino acid selected from the group consisting of histidine, lysine and arginine.

Abstract: A general chiral column with a multipleproline-based chiral stationary phase. Embodiments include chiral stationary phases of the following formula: wherein n is any integer of 2 or greater, and analogs and isomers thereof.

Abstract: The present invention provides a separating agent for enantiomeric isomers, which has an excellent ability to identify asymmetry. That is, the present invention relates to a separating agent for enantiomeric isomers which includes as an effective component a polysaccharide derivative having a bicyclic or more structure having an aromatic, alicyclic, or heterocyclic ring.

Abstract: A general chiral column with a multipleproline-based chiral stationary phase. Embodiments include chiral stationary phases of the following formula: wherein n is any integer of 2 or greater, and analogs and isomers thereof.

Abstract: In one aspect, the invention provides a method for separating a mixture of polynucleotides, such as DNA or RNA, including (a) applying the mixture to a polymeric separation medium having non-polar surfaces, wherein the surfaces are characterized by being substantially free from multivalent cations, such as metal ions, which are free to interfere with polynucleotide separation, and (b) eluting the mixture with a mobile phase containing organic solvent and counter ion agent. In the separation of single-stranded polynucleotides, improved separation is obtained at a temperature effective to fully denature secondary structure within the polynucleotides.

Abstract: Halogenated polymers of vinyl aromatic monomers comprising styrene and divinylbenzene are provided for use in a chromatographic separation method of analytes, wherein said polymer further comprises a hydrocarbyl or halocarbyl substituent comprising from 1 to 1,000,000 carbon atoms, or combinations thereof. Methods of using the halogenated polymers are also provided. The polymers and methods are particularly useful in the separation of nucleic acids labeled with hydrophobic labels such as protecting groups, fluorophores, and the like.

Abstract: The present invention provides silica-based reversed-phase HPLC methods that lead to higher retention of the analytes in the column and longer column lifetimes than usually observed under medium to high pH aqueous mobile phase conditions. The inventive methods comprise eluting the HPLC column using an aqueous mobile phase comprising at least one fluorinated additive. Preferred additives are polyfluorinated alcohols such as 2,2,2-trifluoroethanol and 1,1,1,3,3,3-hexafluoroisopropanol. The methods of the present invention may be used for analyzing, separating, purifying, and/or isolating small organic molecules, natural products, as well as biomolecules such as polypeptides, oligonucleotides and polynucleotides (e.g., DNA fragments).

Abstract: Novel compositions are disclosed for use as a stationary phase in chromatography comprising an inorganic substrate that is modified with at least one silane having the formula R1?-Q?-(CH2)?SiR2?X3-?. In a preferred embodiment, the inorganic substrate is silica gel and is modified with at least two silanes. Methods of preparation and use in chromatographic applications are disclosed. The novel compositions provide superior chromatographic performance, reduced silanol activity, enhanced stability, and reproducibility in preparation and performance.

Abstract: The subject invention includes methods and devices for separating at least two constituents of a highly aqueous fluid. The subject methods include contacting a highly aqueous fluid with a stationary phase having a low density hydrophobic bonded phase under conditions to separate at least two constituents. Also provided are systems that include a stationary phase having a low density hydrophobic bonded phase, a highly aqueous fluid comprising at least two constituents and a device configured to perform liquid chromatography. Kits for use in practicing the subject methods are also provided.

Abstract: A process for purifying a stock Sr-90 solution containing stable and radioactive impurities, holding the purified Sr-90 solution for Y-90 ingrowth, and subsequently extracting the Y-90 from the Sr-90/Y-90 solution. The stock solution is sequentially passed through two thermoxide-type sorbents (T-3 and T-5), which hold the impurities while passing the Sr-90 solution. After ingrowth of Y-90, the Sr-90/Y-90 solution is passed through sorbent T-3, which preferentially sorbs the Y-90 while passing the Sr-90 solution. The Y-90 is then eluted from the T-3 sorbent. The T-3 and T-5 sorbents are specially prepared compounds of zirconium dioxide and titanium dioxide, respectively, that preferentially sorb Y-90 under predetermined conditions of solution pH and NaCl concentration.

Abstract: Ion exchange resins are described that are hydrophilic, crosslinked (meth)acrylic copolymers. The ion exchange resins are macroporous, have a surface area of at least 50 m2/g, and an average particle size of at least 20 micrometers. Additionally, chromatographic columns containing the ion exchange resins, composite materials containing the ion exchange resin, filtration elements containing the ion exchange resin, methods of preparing the ion exchange resins, and methods of separating or purifying negatively or positively charged materials with the ion exchange resins are described.