Abstract: Sewage sludge that has been de-watered to 25% solids is subjected to a combination of shearing and heating that liquefies the sludge, and drops its viscosity to below 12,000 cP. The fact of liquefaction prepares the sludge such that subsequent drying can be done more cost-effectively than hitherto. After drying, the dried sludge can be incinerated.

Abstract: Improved ball mill disruption techniques. In different embodiments, disrupting particles that are not substantially spherical are used. In other embodiments, roughened disrupting particles are used. In other embodiments, larger disrupting particles are used. In each instance, improved disruption can be achieved.

Abstract: The present invention relates to a high pressure homogenizing apparatus and a method thereof for finely dividing a fine solid material or fibrous cellulose of chemical, medical, and resin products in a suspension as a dispersion or emulsification, or finely dividing by crushing cell membranes of fungi with high efficiency. The apparatus of the present invention is free from damage of valves so that its maintenance and control become easy. The high pressure homogenizing apparatus to finely divide a raw material in the suspension includes a high pressure homogenizing device having an orifice, a raw material receiving passage connected to the high pressure homogenizing device, a processing piston, and a processing recess disposed in a receiver.

Abstract: In a biological sample grinding device and method, a bearing having a plurality of rolling elements disposed between a pair of rings is disposed in a container in which a fluid having at least one biological sample particle therein is introduced. With the bearing immersed in the fluid having the biological sample particle therein, one ring of the bearing is rotated relative to the other ring thereof, whereupon the rolling elements roll between the pair of rings and, in response thereto, the biological sample particle is drawn by the fluid between the pair of rings where it is subject to being crushed or ground between at least one of the rolling elements and at least one of the rings. A baffle can be disposed in the container for inhibiting the rotation of the fluid and, hence, the introduction of air into the fluid.

Abstract: An apparatus for comminuting biological specimens includes a receiving component provided with a drive source, at least one sample retainer configured for accommodating at least one biological specimen and constructed and arranged for engagement in the receiving component. The sample retainer includes a first component configured for receiving a specimen prior to comminution and a second component for receiving the specimen after comminution. In addition, the sample retainer is insertable into the receiving component as a single piece.

Abstract: In a biological sample grinding device and method, a bearing having a plurality of rolling elements disposed between a pair of rings is disposed in a container in which a fluid having at least one biological sample particle therein is introduced. With the bearing immersed in the fluid having the biological sample particle therein, one ring of the bearing is rotated relative to the other ring thereof, whereupon the rolling elements roll between the pair of rings and, in response thereto, the biological sample particle is drawn by the fluid between the pair of rings where it is subject to being crushed or ground between at least one of the rolling elements and at least one of the rings. A baffle can be disposed in the container for inhibiting the rotation of the fluid and, hence, the introduction of air into the fluid.

Abstract: A tissue homogenizer. The tissue homogenizer comprises a first chamber, a pair of blades, a first filter and a second filter. The first chamber has a first opening and a second opening. The blades are disposed in the first chamber. The first filter is disposed in the first chamber between the first opening and the blades. The second filter is disposed in the first chamber between the second opening and the blades. A tissue piece is placed between the first filter and the second filter cut by the blade, and moved by a fluid through the second filter to generate homogenized tissue pieces.

Abstract: Method for disrupting S. Cerevisiae yeast cells in an aqueous solution, by means of a continuous process under high pressure (up to 4000 bar), using a homogenizer. The suspension to be processed passes through a homogenizing valve with a “sharp edge” or “knife edge” passage head in order to achieve a 100% cell disruption rate with a single passage, at a dynamic pressure equal to or above 2000 bar. The “sharp edge” or “knife edge” profile of the passage head (4) is characterized by an inside diameter (9) of 10.9-14 mm and an outside diameter (10) of 11.9 mm-15 mm, and operates at a flow rate of 100-500 liters/hour and a dynamic pressure of more than 2000 bar.

Abstract: An apparatus and a method for isolating a biologic product, such as plasmid DNA, from cells. The method involves lysing cells in a controlled manner separate insoluble components from a fluid lysate containing cellular components of interest, followed by membrane chromatographic techniques to purify the cellular components of interest. The process utilizes a unique lysis apparatus, ion exchange and, optionally, hydrophobic interaction chromatography membranes in cartridge form, and ultrafiltration. The process can be applied to any biologic product extracted from a cellular source. The process uses a lysis apparatus, including a high shear, low residence-time mixer for advantageously mixing a cell suspension with a lysis solution, a hold time that denatures impurities, and an air-sparging bubble mixer that gently yet thoroughly mixes lysed cells with a neutralization/precipitation buffer and floats compacted precipitated cellular material.

Abstract: Paper shredders are maintained using lubrication substrates that have been treated with or that carry a lubricant. The lubrication substrate is fed through the shredding mechanism of a paper shredder. Rather than requiring disassembly of the paper shredder, maintenance and lubrication can be performed by passing the lubrication substrate through the shredding mechanism. The lubrication substrates can take any of a variety of forms, such as a tissue that is encased in a cellophane or plastic envelope and is impregnated with oil. The lubrication substrates can instead have an array of small tubes that carry oil. In another example, the lubrication substrates can take for them of a bubble sheet that carries oil within the bubbles.

Abstract: A flexible automated apparatus for isolating and purifying viruses, proteins and peptides of interest from a plant material is disclosed, the apparatus being applicable for large scale purification and isolation of such substances from plant material. The flexible automated apparatus provides an efficient apparatus for isolating viruses, proteins and peptides of interest with little waste material. The automated apparatus for isolating viruses, proteins and peptides of interest includes a grinding apparatus for homogenizing a plant to produce a green juice, a means for adjusting the pH of and heating the green juice, a means for separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.

Abstract: A container for holding cells or viruses for disruption comprises a chamber defined by two spaced apart, opposing major walls and side walls connecting the major walls to each other. At least one of the major walls has an external surface to which the transducer may be coupled and is sufficiently flexible to flex in response to vibratory motion of the transducer. The container also has at least one port for introducing the cells or viruses into the chamber. In some embodiments, the chamber contains beads for aiding the disruption of the cells or viruses.

Abstract: A method and device for performing lysing on a cell-containing fluid, in which the fluid flows through a vibrating micromachined tube to physically rupture the cell walls (mechanical lysis), and/or to mix, agitate or homogenize the fluid during chemical lysis, and/or to mix, agitate or homogenize the lysate for analysis or other processing after lysing. The tube includes a freestanding portion spaced apart from a surface of a substrate on which the tube is formed. The device further includes means for vibrating the freestanding portion of the tube at a level sufficient to rupture the walls of cells in a fluid flowing through the freestanding portion (for mechanical lysing) or to mix the fluid and a chemical lysing additive within the freestanding portion (for chemical lysing).

Abstract: A device and process for pulverizing solid materials is provided, which generates vertical and horizontal motion of grinding beads in multiple tube samples containing a sample to be pulverized.

Abstract: A tubular vessel is loaded with a combination of grinding media and a material to be ground. The vessel is capped to contain the grinding media and material therein. Grinding of the contained material is effectuated by reciprocating the capped vessel in a direction parallel to its longitudinal axis. The grinding media may comprise either a ball or a slug, and may further utilizing a plurality of balls, perhaps of different sizes. To increase volume, a plurality of vessels may be gathered together into a sample holder. The sample holder is them reciprocated in a direction parallel to the axes of the included vessels.

Abstract: The present invention relates to a method of preparing adsorbent particles formed from expanded plastics materials, said particles comprising cellular structures that are open at their surface, characterized in that a material constituted by agglomerated beads of said expanded material and having smooth surfaces is rasped into fine chips. The present invention also relates to adsorbent particles formed from an expanded plastics material comprising cellular structures that are open at their surface, characterized in that they are in the form of fine chips, preferably with a dimension of 0.8 mm to 4 mm, said open cellular structures represent more than 80% of the visible surface area of said chips. In a method of treating soil polluted with hydrocarbons, the following steps are carried out, in which: 1) said polluted soil is mixed with adsorbent particles according to the invention; and 2) said adsorbent particles charged with hydrocarbons are separated from the depolluted soil.

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The chamber is defined by at least one wall having an external surface for contacting a transducer device. The transducer device has a vibrating surface for contacting the wall and for vibrating at an operating frequency and amplitude sufficient to generate pressure waves or pressure pulses in the chamber. The transducer device is coupled to the wall with a preload force sufficient to create a stress within the wall. The natural frequency of the wall, when the wall is stressed by the preload force, is equal to the operating frequency of the transducer device or differs from the operating frequency by less than 50% of the operating frequency.

Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.

Abstract: A device and method are disclosed for selective exposure of a biological sample, preferably biological cell material, to sound waves. The device is provided with a receptacle for the sample, in which the biological sample is in a suspended form, and having an electroacoustic transducer device, which generates sound waves and which is disposed outside the receptacle of the sample in such a manner that sound-wave coupling into said sample occurs through the wall of said receptacle.

Abstract: A deconstituting device for the preparation of biological samples comprising: a container (2) in the form of a for holding the tissue to be deconstituted; a shaft (26) mounted for rotation inside the said container (2) with a blade (28) on the end inside the container, the said shaft (26) being supported axially by ball coupling means (30, 34) and having engagement means (36) on its end outside the container for coupling the said shaft to motor means; the device being used in automatic apparatus, which includes: at least one support element (54) with a plurality of housings for receiving the container; a deconstitution station (50) which includes at least one motor (52) with a drive shaft (55) for engaging the said engagement means (36) of the shaft (26) of the deconstituting device, this motor being movable between a position disengaged from the said shaft (26) and one engaging it, and; conveyor means (66, 68 and 78) for transporting the said support element (54) to the deconstitution station.

Abstract: A device for grinding biological samples to extract DNA, RNA and proteins including a multiplicity of rotary hammers supported by a hammer-carrier block and a lower mobile tray, the tray being movable between an operating position close to the hammer-carrier block and a rest position away from the hammer-carrier block, each of the hammers comprising at least one conduit for distribution of liquid.

Abstract: The present invention relates to a method of reducing particle size by the use of a supercritical gas, such as carbon dioxide or a refrigerant. The material is swollen by the supercritical gas at a high pressure. After the material has been allowed to swell under a high pressure, the pressure is rapidly dropped. When the external pressure is rapidly dropped, the material explodes as the supercritical gas absorbed into the material rapidly expands outward.

Abstract: There is a method for the mechanical disintegration of biogenic sewage sludge, for which the sewage sludge, in an initial state, has microorganisms in the form of cells and a solid, which is formed essentially from aggregates of cells and suspended materials, and for which a disintegration process (3, 4) causes a destruction of aggregates and a destruction of cells. It is desirable to improve the disintegration. This is achieved in that, in a first disintegration step 3, primary destruction of aggregates and, in a subsequent, separate second disintegration step 4, primary destruction of cells is caused. Due to the use of two separate disintegration processes, an appreciable improvement in the disintegration is achieved, since fewer aggregates and more cells are supplied to the second disintegration process. The increased destruction of cells brings about the improvement in the disintegration.

Abstract: The present invention relates to a method of reducing particle size by the use of a supercritical fluid, generally carbon dioxide. The material is swollen by the supercritical carbon dioxide at a high pressure. After the material has been allowed to swell under a high pressure, the pressure is rapidly dropped. When the external pressure is rapidly dropped, the material explodes as the supercritical gas absorbed into the material rapidly expands outward.

Abstract: A container for holding cells or viruses for disruption comprises a chamber defined by two spaced apart, opposing major walls and side walls connecting the major walls to each other. At least one of the major walls has an external surface to which the transducer may be coupled and is sufficiently flexible to flex in response to vibratory motion of the transducer. The container also has at least one port for introducing the cells or viruses into the chamber. In some embodiments, the chamber contains beads for aiding the disruption of the cells or viruses.

Abstract: A method and device for performing lysing on a cell-containing fluid, in which the fluid flows through a vibrating micromachined tube to physically rupture the cell walls (mechanical lysis), and/or to mix, agitate or homogenize the fluid during chemical lysis, and/or to mix, agitate or homogenize the lysate for analysis or other processing after lysing. The tube includes a freestanding portion spaced apart from a surface of a substrate on which the tube is formed. The device further includes means for vibrating the freestanding portion of the tube at a level sufficient to rupture the walls of cells in a fluid flowing through the freestanding portion (for mechanical lysing) or to mix the fluid and a chemical lysing additive within the freestanding portion (for chemical lysing).

Abstract: A method of disrupting biological material includes drying particulate material, mixing the material with a gas under pressure, releasing the pressure explosively and collecting the resultant product. The biological starting material is any particulate material and includes: cells with membranes, cells with rigid cell walls, non-cellular biological material, intra-cellular material, and unbounded homogenous material. Apparatus for batch, semi-continuous and continuous operation of the method is provided. Included is a chamber with at least one inlet valve and at least one outlet valve and collection means. The chamber is capable of withstanding at least 800 bar, preferably 30 bar pressure. The particle size of the starting material is in the range 0.1 to 2000 &mgr;m and of the resultant product, less than 2 &mgr;m.

Abstract: In order to inexpensive construction materials effectively utilizing timber resources and realizing any desired properties by using so-called low-quality materials including slim timbers, old timbers, wood cuttings produced by lumbering, bamboo, and so forth, wood, bamboo and other wooden source materials are split into fragments along their fibers by water vapor explosion, and such explosive-split fragments are shaped and hardened by adding an adhesive, mortar or expandable resin into a new wooden material such as multi-layered board, cement board or foamed resin board of explosive-split fragments. The explosive-split fragments are also usable in various fields other than fabrication of the new material.

Abstract: The present invention provides an apparatus and method for disrupting cells or viruses to release the nucleic acid therefrom. The apparatus includes a container having a chamber for holding the cells or viruses. The apparatus also includes an ultrasonic transducer for contacting a wall of the chamber and for transmitting ultrasonic energy into the chamber through the wall. A support structure holds the container and the transducer against each other such that the transducer contacts the wall of the chamber. The support structure includes an elastic body, such as a spring, for applying to the container or to the transducer a substantially constant force to press together the transducer and the wall. The chamber also preferably contains beads for enhancing the disruption of the cells or viruses. The apparatus performs rapid and consistent lysis of cells or viruses, often in as little time as 5 to 10 seconds.

Abstract: The present invention relates to a method of reducing particle size by the use of a supercritical fluid, generally carbon dioxide. The material is swollen by the supercritical carbon dioxide at a high pressure. After the material has been allowed to swell under a high pressure, the pressure is rapidly dropped. When the external pressure is rapidly dropped, the material explodes as the supercritical gas absorbed into the material rapidly expands outward.

Abstract: A method of liberating intracellular matter from biological material having cells with cell walls includes subjecting the biological material to rapid pressure increases and decreases, and exceeding the elastic limit of the cell walls with the pressure increases and decreases, thereby opening the cell walls and liberating the intracellular material from the cells. This produces a heterogenous mixture of cell wall fragments and the intracellular material. Where the biological material includes pieces of plant animal or fungal material, the method can further include separating the cells of the pieces from each other with the pressure increases and decreases when the elastic limit of intercellular bonds are exceeded. Water and volatiles in the biological material is liberated and vaporized, producing a substantially dry mixture having a lower water content than the original material.

Abstract: A device for grinding biological samples to extract DNA, RNA and proteins including a multiplicity of rotary hammers supported by a hammer-carrier block and a lower mobile tray, the tray being movable between an operating position close to the hammer-carrier block and a rest position away from the hammer-carrier block, each of the hammers comprising at least one conduit for distribution of liquid.

Abstract: There is a method for the mechanical disintegration of biogenic sewage sludge, for which the sewage sludge, in an initial state, has microorganisms in the form of cells and a solid, which is formed essentially from aggregates of cells and suspended materials, and for which a disintegration process (3, 4) causes a destruction of aggregates and a destruction of cells. It is desirable to improve the disintegration. This is achieved in that, in a first disintegration step 3, primary destruction of aggregates and, in a subsequent, separate second disintegration step 4, primary destruction of cells is caused. Due to the use of two separate disintegration processes, an appreciable improvement in the disintegration is achieved, since fewer aggregates and more cells are supplied to the second disintegration process. The increased destruction of cells brings about the improvement in the disintegration.

Abstract: A cartridge for separating a desired analyte from a fluid sample has a sample flow path and a lysing chamber in the sample flow path. The lysing chamber contains at least one filter for capturing cells or viruses from the sample as the sample flows through the lysing chamber. Beads are also disposed in the lysing chamber for rupturing the cells or viruses to release the analyte therefrom. An analyte flow path extends from the lysing chamber and diverges from the sample flow path. The analyte flow path preferably leads to a reaction chamber for chemically reacting and optically detecting the analyte. The cartridge also includes at least one flow controller (e.g., valves) for directing the sample into the waste chamber after the sample flows through the lysing chamber and for directing the analyte separated from the sample into the analyte flow path.

Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.

Abstract: A micro-sonicator for spore lysis. Using micromachining technology, the micro-sonicator uses ultrasonic excitation of spores to perform spore and cell lysis. The micro-sonicator comprises a container with a cavity therein for retaining the sample in an ultrasonic transmission medium, the cavity being closed by a silicon membrane to which an electrode and piezoelectric material are attached, with the electrode and piezoelectric material being electrically connected to an AC signal generator which causes the membrane to flex and vibrate at the frequency of the applied voltage.

Abstract: A method is described for the continuous disintegration of microbial sludge in a sewage clarification or sewage treatment plant wherein the microbial sludge is decomposed biologically by bacteria and the resultant sludge (5) is exposed to sonic radiation in a treatment vessel (1) in order to split the cell walls of the microorganisms. With that, the sonic radiation shall be generated asymnetrically in the treatment vessel (1). The intensity of the sonic exposure lies in an area of 500 to 1500 W/m2 and the sludge (5) is fed with a mean dwell-time of 2 to 4 minutes in counter-flow through the treatment vessel (1). The device for carrying out the method possesses a treatment vessel (1) with an eccentrically arranged sonic resonator arranged within (7).

Abstract: A process for liquefying microorganisms present in biosolids, such as waste activated sludge, generated by municipal or industrial wastewater treatment plants is disclosed. The process includes the step of passing a slurry of the sludge at a high pressure through a nozzle having a restricted flow area to cause liquefication of the microorganisms as they are discharged from the nozzle. The pressure drop across the nozzle preferably exceeds 5,000 psi. At least part of the liquefied microorganisms may be recirculated to the treatment facility to feed nutrients to the wastewater treatment process. Optionally, the liquefied microorganisms may be anaerobically converted to methane and carbon dioxide. For optimum results, the sludge is subjected to pH adjustment and/or maceration prior to being pumped through the high pressure nozzle.

Abstract: A method for rupturing microalgae in an aqueous suspension is disclosed. In one embodiment the aqueous suspension is passed through a constriction into a liquid phase at a pressure sufficient to rupture the cells by circulating the aqueous suspension through a constriction in a pump loop at a pressure and a percent recycle sufficient to rupture the cells. Cells of the alga Dunaliella salina can be ruptured by the method of the invention to promote froth flotation and mechanical filtration of the cells for recovery of mixed carotenoids.

Abstract: A process and system are disclosed for recovering mixed carotenoids from the alga Dunaliella salina. The harvested cells are ruptured, typically by circulating the algal suspension at high pressure through a pump loop. The cells can then be dewatered by absorptive bubble separation techniques, including a froth floatation circuit that has a roughing zone and a concentrating zone. If further concentration is desired, the algal concentrate can be mechanically filtered in a cross flow microfiltration unit in the absence of flocculating agents with substantially no loss of carotenoids in the permeate. Various methods for extracting mixed carotenoids and other components from the algae are disclosed, including dense gas extraction, and extractions with natural and synthetic flavorants, and edible oils.

Abstract: An apparatus and method for preparing tissue samples for DNA, RNA or protein extraction of the present invention includes a reciprocating saw mounted to a frame and connected to a plurality of tissue sample containers containing tissue samples and agitation members. By activating the reciprocating saw, the tissue samples are ground by the agitation members. A control circuit is connected to the saw for controlling the speed and duration of the agitation.

Abstract: Devices and corresponding mechanical non-fermentive methods for isolating seeds from fruits or vegetables and for releasing and separating the isolated seeds from their coats, according to one embodiment the device includes a first station including a first chamber and a second chamber at least partly separated therebetween by a perforation element, the first chamber engages a mixing mechanism for circulating a coated seeds including liquid mixture such that the coated seeds are scraped against the perforation element and passing through the element into the second chamber, the device further includes a second station including a third chamber engaging a vortexing mechanism for vortexing the liquid mixture, such that the seeds resulting from the first station are scraped against one another, thereby releasing the seeds from their coats, the device further includes drains for separating the released seed coats from the naked seeds.

Abstract: An apparatus for grinding specimens, comprises a container, a grinder, a closure for the container, and a seal contained in the closure. The container has an interior grinding surface and contains an opening through which specimens are introduced for grinding. The grinder has a grinding head and an actuator arm extending from the grinding head. The grinding head is adapted to engage the interior grinding surface of the container. Grinding is effected by moving the grinding head against the grinding surface, using the actuator arm. The closure is adapted to close the container and enclose the grinding head therein. The closure contains a passage through which the actuator arm extends when the closure is enclosing the grinding head. The seal is contained in the closure and coaxially aligned with the passage of the closure, such that the actuator arm slidably engages the seal when the arm passes through the closure.

Abstract: A substantially odorless and sterilized fertilizer in pellet form is produced by combining a dry organic waste material and a dry binder material, then combining the resulting dry mixture with steam, water and/or further organic waste in the form of sludge. The resulting material is provided to an extruder for a more thorough, dispersive mixing, a pressure increase to at least 100 psi, and heating above the glass transition temperature of the binder, and more preferably to at least about 125.degree. C. to substantially sterilize the material mass. The extruded material is forced through a die and released to an ambient environment, rapidly reducing pressure of the material and thereby lysing spores and microorganisms surviving conditions within the extruder. The emerging material is segmented and dried, or segmented and tumbled to form pellets, then dried.

Abstract: An insertless perforated mill roll body adapted to be detachably sleeved upon a roller shaft for the grinding of a fluid-containing material such as sugar cane and extracting fluid such as sucrose juice therefrom. The insertless perforated mill roll body comprises a plurality of shish-ke-bab-like fluid channel strings to be encased in the roll body, each fluid channel string comprises a hollow fluid channel preferably defined by a channel wall member which generally extends between the two axial ends of the roll body with a plurality of fluid passage members affixed thereto. The roll body is formed by casting a castable material such as cast iron or steel to enclose the fluid channel strings, whereupon a hollow center bore is provided to receive the shaft therethrough. Each fluid passage member contains at least one generally radially extending fluid passage to allow communication between the outer periphery of the mill roll body and the fluid channel.

Abstract: A process capable of being continuously carried out, for disintegrating cell material in the form of dispersions or suspensions in water for the purpose of obtaining cell constituent. Selected parameters permit avoidance of use of solid ultrasonication activators and the establishment of a particular geometry form for the acoustic irradiation container. The parameters include sonotrode immersion angle, length of immersion, ratio of extent of immersion of the sonotrode relative to the acoustic irradiation volume and the ratio of extent of immersion to the solid matter content of the medium to be sonicated.

Abstract: The shelf-stability of carrot root material is enhanced by blanching the material, milling it, and then subjecting it to a decreasing pressure which is sufficiently rapid to cause rupturing of the cell walls of contaminating organisms in the product. The latter is effected by passing the product through a homogenizing valve (20). The treated product is then packaged by an aseptic packaging machine (22), to keep it from re-contamination by contaminating organisms.

Abstract: An improved apparatus for effectively disrupting biological samples contained in cuvettes to which beads have been added. In the apparatus, a special arm/bearing subassembly is driven and oscillated by a motor in a manner to attain cellular disruption of the biological samples without degradation of their cellular components. In the preferred form, the special arm/bearing subassembly has a cam, bearings, and a bearing sleeve which cooperate with a motor drive shaft to rotate a yoke with two arms holding four cuvettes. For increased safety and environmental protection, special sample retainers can be provided to better secure the cuvettes and the arm/bearing subassembly is enclosed in a sample chamber which provides a secondary containment compartment that contain any spillage.

Abstract: Method for disposing of absorbent material impregnated with waste. The capillary retention capability of the absorbent material impregnated with waste is destroyed, thereby releasing the waste retained in the material. The absorbent material and released waste form a mixture for disposal at a boiler, industrial furnace or incinerator.