Abstract: The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples.

Abstract: A device for imparting an orbital angular momentum to a charged particle wave propagating along a beam axis in a charged particle beam generating apparatus is described. The device comprises a support element having a target region adapted for transmitting a charged particle wave propagating along a beam axis and an induction means for inducing a magnetic flux along an elongated profile having a free end portion located in the target region and the induction means is adapted for providing a magnetic flux in the elongated profile in order to induce an angular gradient, relative to the beam axis, of the phase of the charged particle wave when transmitted through the target region. A corresponding method is also disclosed, as well as the use thereof in electron microscopy.

Abstract: Shutter gate grid is a structure wherein first and second disc-shaped members made of metal with an insulation sheet member sandwiched therebetween are integrally joined by means of insulative screws. Gate grids with conductive wires secured to the central openings thereof are fused onto the disc-shaped members; screw insertion holes formed in the second disc-shaped member are fan-shaped and have play in the direction of rotation, making it possible to adjust the parallelism of the conductive wires of the two members by finely adjusting the rotational position when tightening the screws. Moreover, diffusion gas can be made to flow through ventilation holes provided at common locations on each member, thus making it possible to provide uniformity of diffusion gas flow in the drift region.

Abstract: The present invention provides a radio frequency (RF) power supply in a mass spectrometer. The power supply provides an RF signal to electrodes of a storage device to create a trapping field. The RF field is usually collapsed prior to ion ejection. In an illustrative embodiment the RF power supply includes a RF signal supply; a coil arranged to receive the signal provided by the RF signal supply and to provide an output RF signal for supply to electrodes of an ion storage device; and a shunt including a switch operative to switch between a first open position and a second closed position in which the shunt shorts the coil output.

Abstract: A mass spectrometer having a mass filter which applies a transient voltage profile to accelerate ion packets. The voltage profile is chosen to have a functional form which imparts each ion species with a kinetic energy which is larger the larger the mass-to-charge ratio and a velocity which is smaller the larger the mass-to-charge ratio. The ions are detected in an ion detector which discriminates between different ion species based on their kinetic energy and taking account of the functional form of the voltage profile. Suitable voltage profiles include periodic functions such as sinusoids, triangles and sawtooths, which allow the amplification of drive pulses in the mass filter to be carried out with narrow band amplification stages, which are simple and inexpensive to construct.

Abstract: LC/MS data generated by an LC/MS system is analyzed to determine groupings of ions associated with originating molecules. Ions are grouped initially according to retention time, for example, using retention time or chromatographic peaks in mass chromatograms. After initial groupings are determined based on retention time, ion peak shapes are compared to determine whether ions should be excluded. Ions having peak shapes not matching other ions, or alternatively a reference peak shape, are excluded from the group.

Abstract: The length of a delay time d from a suspension period starting point t1 until the application of a pulse voltage is begun is changed according to the length of the suspension period during which no data is collected at the time of m/z switching. It is thus ensured that the amount of product ions can be reliably restored at a suspension period termination point t2. In addition, the peak value of the pulse voltage is also changed according to the ionic strength immediately before entering the suspension period. The ion removal rate is thus increased when the amount of remaining ions is high, and the amount of remaining ions is reliably brought to zero within the same pulse width. As a result, crosstalk can be completely removed.

Abstract: The present invention provides a timing device, especially a timing device for use in mass spectrometers, for example TOF mass spectrometers, for processing trigger signal data containing a trigger signal indicating the occurrence of a trigger event, the timing device having: a trigger signal deserializer configured to receive trigger signal data containing a trigger signal indicating the occurrence of a trigger event as serial data and to output the trigger signal data as parallel data, and wherein suitably the timing device has a processing means configured to process trigger signal data outputted by the trigger signal deserializer as parallel data.

Abstract: In a mass cytometer or mass spectrometer, a sample of elemental tagged particles is transferred from a dispersion to a gas flow through a carrier aerosol spray for atomization and ionization by inductively coupled plasma (ICP) source. The configuration of the sample transfer apparatus allow for total consumption of the sample by passing the sample spray through a deceleration stage to decelerate the spray of particles from its high velocity expansion. Following the deceleration stage, the decelerated sample of particles can be accelerated and focused through an acceleration stage for transferring into the ICP. This effectively improves the particle transfer between the sample spray and the ICP.

Abstract: The invention generally relates to systems and methods for transferring ions for analysis. In certain embodiments, the invention provides a system for analyzing a sample including an ionizing source for converting molecules of a sample into gas phase ions in a region at about atmospheric pressure, an ion analysis device, and an ion transfer member operably coupled to a gas flow generating device, in which the gas flow generating device produces a laminar gas flow that transfers the gas phase ions through the ion transfer member to an inlet of the ion analysis device.

Abstract: A mass spectrometer is disclosed wherein an ion signal is split into a first and second signal. The first and second signals are multiplied by different gains and are digitized. Arrival time and intensity pairs are calculated for both digitized signals and the resulting time and intensity pairs are combined to form a high dynamic range spectrum. The spectrum is then combined with other corresponding spectra to form a summed spectrum.

Abstract: When a specimen from a specimen ionizing unit is not sufficiently ionized, is caused to remain in sites other than a pore in an introducing section and be deposited as a product such as an oxide or carbide, which causes a deterioration in the performance of the mass spectrometry device. The mass spectrometry device has a specimen ionizing section for ionizing a specimen, a specimen-introduction regulating chamber into which ions of the ionized specimen are introduced, a differential evacuation chamber located downstream of the specimen-introduction regulating chamber, and an analyzing section located at the downstream side of the differential evacuation chamber, in which a discharge generating means is formed for generating an electric discharge inside the specimen-introduction regulating chamber and/or the differential evacuation chamber.

Abstract: A Time of Flight mass analyser is disclosed comprising one or more devices arranged and adapted to correct for tilt in an isochronous plane of ions and to adjust the isochronous plane of the ions so as to be parallel with the plane of detection in an ion detector.

Abstract: An ion detector for a Time of Flight mass spectrometer is disclosed comprising a single Microchannel Plate 1 which is arranged to receive ions 2 and output electrons 3. The electrons 3 are directed onto an array of photodiodes 4 which directly detects the electrons 3. The output from each photodiode 4 is connected to a separate Time to Digital Converter provided on an ASIC 5.

Abstract: An ion trap is provided between a collision cell and a time-of-flight mass separator. During a time period in which precursor ions derived from the same compound are selected with a quadrupole mass filter, a collision energy is changed from one to another. Various product ions that are produced by dissociation respectively under collision energies of the plurality of stages and precursor ions that are not dissociated are temporarily trapped in the ion trap, and are ejected in a packet form in the state where these ions are mixed, and are introduced into the time-of-flight mass separator 6 to be subjected to a mass spectrometry. Thereby, in a data processing unit, one MS/MS spectrum in which product ions produced in various dissociation modes under various CID conditions appear is created.

Abstract: A method, apparatus and algorithms are disclosed for operating an open electrostatic trap (E-trap) or a multi-pass TOF mass spectrometer with an extended flight path. A string of start pulses with non equal time intervals is employed for triggering ion packet injection into the analyzer, a long spectrum is acquired to accept ions from the entire string and a true spectrum is reconstructed by eliminating or accounting overlapping signals at the data analysis stage while using logical analysis of peak groups. The method is particularly useful for tandem mass spectrometry wherein spectra are sparse. The method improves the duty cycle, the dynamic range and the space charge throughput of the analyzer and of the detector, so as the response time of the E-trap analyzer. It allows flight extension without degrading E-trap sensitivity.

Abstract: LC/MS data generated by an LC/MS system is analyzed to determine groupings of ions associated with originating molecules. Ions are grouped initially according to retention time, for example, using retention time or chromatographic peaks in mass chromatograms. After initial groupings are determined based on retention time, ion peak shapes are compared to determine whether ions should be excluded. Ions having peak shapes not matching other ions, or alternatively a reference peak shape, are excluded from the group.

Abstract: An electrical probe assembly includes a flexible circuit. A plurality of electrically conductive regions is on a first side of the flexible circuit. The flexible circuit is arranged about an axis formed by rolling the flexible circuit such that the electrically conductive regions form a plurality of isolated electrically conductive bands.

Abstract: Systems and methods are used to analyze a sample using variable mass selection window widths. A tandem mass spectrometer is instructed to perform at least two fragmentation scans of a sample with different mass selection window widths using a processor. The tandem mass spectrometer includes a mass analyzer that allows variable mass selection window widths. The selection of the different mass selection window widths can be based on one or more properties of sample compounds. The properties may include a sample compound molecular weight distribution that is calculated from a molecular weight distribution of expected compounds or is determined from a list of molecular weights for one or more known compounds. The tandem mass spectrometer can also be instructed to perform an analysis of the sample before instructing the tandem mass spectrometer to perform the at least two fragmentation scans of the sample.

Abstract: The invention generally relates to systems and methods for transferring ions for analysis. In certain embodiments, the invention provides a system for analyzing a sample including an ionizing source for converting molecules of a sample into gas phase ions in a region at about atmospheric pressure, an ion analysis device, and an ion transfer member operably coupled to a gas flow generating device, in which the gas flow generating device produces a laminar gas flow that transfers the gas phase ions through the ion transfer member to an inlet of the ion analysis device.

Abstract: A mass spectrometer is disclosed comprising two vacuum chambers maintained at different pressures. The two vacuum chambers are interconnected by a differential pumping aperture. The effective area of the opening between the two vacuum chambers may be varied by rotating a disk having an aperture in front of the differential pumping aperture so as to vary the gas flow rate through the opening and between the two chambers.

Abstract: An ion guide that transports ions from an ion source at generally a high-pressure level to a mass analyzer at generally a low-pressure level has a plurality of identical electrodes fabricated with protruding elements that forming an ion tunnel or an ion funnel, when the electrodes are assembled around a common longitudinal axis. The protruding elements allow the generation of the radio frequency field necessary to radially confine ions. Each electrode may be machined from a solid block of conductive material, such as metal. The disclosed arrangement greatly simplifies the manufacturing process, reducing cost, and improving robustness and reliability of the ion guide itself.

Abstract: A method and apparatus for transmission of charged particles along a laser-induced conduction path of concentric plasma channels in atmosphere. The apparatus comprises a high power laser array in operable communication with a high energy output means to accomplish initiation of at least two concentric plasma channels in atmosphere, a second energy source for outputting the charged particles to be transmitted, and means for introducing the charged particles to be transmitted into the wall of at least one of the laser-induced conduction channels. Other embodiments further include means for inducing the energy across the conduction path to a target capable of receiving and storing the energy, and a plurality of charging rods bearing a negative or positive charge and in communication with each conductive channel for shaping and stabilizing the charge transmitted therethrough.

Abstract: Mass spectrometers and methods for measuring information about samples using mass spectrometry are disclosed.

Abstract: A mass spectrometer is disclosed wherein an ion signal is split into a first and second signal. The first and second signals are multiplied by different gains and are digitised. Arrival time and intensity pairs are calculated for both digitised signals and the resulting time and intensity pairs are combined to form a high dynamic range spectrum. The spectrum is then combined with other corresponding spectra to form a summed spectrum.

Abstract: According to one embodiment, a laser ion source is configured to generate ions by application of a laser beam, the laser ion source including a case to be evacuated, an irradiation box disposed in the case and including a target which generates ions by irradiation of laser light, an ion beam extraction mechanism which electrostatically extracts ions from the irradiation box and guides the ions outside the case as an ion beam, a valve provided to an ion beam outlet of the case, the valve being opened at ion beam emission and being closed at other times, and a shutter provided between the valve and the irradiation box, the shutter being intermittently opened at ion beam emission and being closed at other times.

Abstract: A time-of-flight mass spectrometer includes a sample plate that supports a sample for analysis. A pulsed ion source generates a pulse of ions from the sample positioned on the sample plate. An ion accelerator receives the pulse of ions generated by the pulsed ion source and accelerates the ions. An ion detector includes an input in a flight path of the accelerated ions emerging from the field-free drift space and an output that is electrically connected to the sample plate. The ion detector converts the detected ions into a pulse of electrons.

Abstract: A distance-of-flight mass spectrometer (DOFMS) includes an ion source, a field-free region, an extraction region in which ions are accelerated, and a spatially-selective detector for spatially selectively detecting ions extracted by the extraction region. A method for operating a distance-of-flight mass spectrometer DOFMS comprises controlling a detection time in such a way as to permit ions with progressively greater mass-to-charge (m/z) ratios to enter the extraction region of the DOFMS at positions which will permit the ions with progressively greater m/z ratios to enter the detector of the DOFMS, generating a component mass spectrum at each selected value of detection time, and then assembling a composite mass spectrum by shifting the distance-of-flight axis of each component mass spectrum by a distance corresponding to the change in detection time.

Abstract: A mass spectrometer is disclosed comprising a first storage ion trap arranged upstream of a high performance analytical ion trap. According to an embodiment ions are simultaneously scanned from both the first and second ion trap. At any instant in time the quantity of charge present within the second ion trap is limited or restricted so that the second ion trap does not suffer from space charge saturation effects and hence the performance of the second ion trap is not degraded.

Abstract: A mass spectrometer is disclosed comprising a RF ion guide wherein in a mode of operation a continuous, quasi-continuous or pulsed beam of ions is orthogonally sampled from the ion guide and wherein the continuous, quasi-continuous or pulsed beam of ions is not axially trapped or otherwise axially confined within the RF ion guide. The ion guide is maintained, in use, at a pressure selected from the group consisting of: (i) 0.0001-0.001 mbar; (ii) 0.001-0.01 mbar; (iii) 0.01-0.1 mbar; (iv) 0.1-1 mbar; (v) 1-10 mbar; (vi) 10-100 mbar; and (vii) >100 mbar.

Abstract: The present invention provides a timing device, especially a timing device for use in mass spectrometers, for example TOF mass spectrometers, for processing trigger signal data containing a trigger signal indicating the occurrence of a trigger event, the timing device having: a trigger signal deserialiser configured to receive trigger signal data containing a trigger signal indicating the occurrence of a trigger event as serial data and to output the trigger signal data as parallel data, and wherein suitably the timing device has a processing means configured to process trigger signal data outputted by the trigger signal deserialiser as parallel data.

Abstract: The length of a delay time d from a suspension period starting point t1 until the application of a pulse voltage is begun is changed according to the length of the suspension period during which no data is collected at the time of m/z switching. It is thus ensured that the amount of product ions can be reliably restored at a suspension period termination point t2. In addition, the peak value of the pulse voltage is also changed according to the ionic strength immediately before entering the suspension period. The ion removal rate is thus increased when the amount of remaining ions is high, and the amount of remaining ions is reliably brought to zero within the same pulse width. As a result, crosstalk can be completely removed.

Abstract: The invention generally relates to systems and methods for transferring ions for analysis. In certain embodiments, the invention provides a system for analyzing a sample including an ionizing source for converting molecules of a sample into gas phase ions in a region at about atmospheric pressure, an ion analysis device, and an ion transfer member operably coupled to a gas flow generating device, in which the gas flow generating device produces a laminar gas flow that transfers the gas phase ions through the ion transfer member to an inlet of the ion analysis device.

Abstract: Methods are provided for detecting the amount of one or more CAH panel analytes (i.e., pregnenolone, 17-OH pregnenolone, progesterone, 17-OH progesterone, dehydroepiandrosterone (DHEA), androstenedione, testosterone, deoxycorticosterone, 11-deoxycortisol, and cortisol) in a sample by mass spectrometry. The methods generally involve ionizing one or more CAH panel analytes in a sample and quantifying the generated ions to determine the amount of one or more CAH panel analytes in the sample. In methods where amounts of multiple CAH panel analytes are detected, the amounts of multiple analytes are detected in the same sample injection.

Abstract: An ion trap comprises a ring electrode and opposite first and second endcap electrodes situated at opposite ends of the ring electrode. A waveform generator is configured to vary both frequency and amplitude of an AC waveform applied across the first and second endcap electrodes as a function of time, thereby exciting ions with a band of resonant secular frequencies substantially without exciting ions with adjacent secular frequencies.

Abstract: An ion mobility tube comprises an ionization source chamber having a center ionization source chamber hole, an ion door, a mobility region unit having a center mobility tube chamber, a constraining grid, and a Faraday disk, and the ionization source chamber, the ion door, the mobility region unit, the constraining grid, and the Faraday disk are laminated together in sequence in a front-rear direction, wherein the mobility region unit comprises a first insulator and first metal electrode sheets concentrically fixed to a front surface and a back surface of the first insulator respectively. The mobility region unit comprises the first insulator and the first metal electrode sheets which are integral. Therefore, the ion mobility tube is advantageous in simplified manufacturing, and convenient for detachment and assembly.

Abstract: An ion mobility spectrometer (IMS) for the detection of trace gaseous molecular compounds dissolved or suspended in a carrier gas, particularly in ambient air, without preconcentration or the trapping of analyte particles. The IMS of the invention comprises an ionization volume of greater than 5 cm3 and preferably greater than 100 cm3. The larger size ionizers of this invention enable analysis of trace (<1 ppb) of sample compounds in the gas phase. To facilitate efficient ion motion through the large volume ionization and reaction regions of the IMS, an electric field gradient can be provided in the ionization region or in both the ionization and reaction regions. The systems can be implemented with radioactive ionization sources, corona discharge ion sources or ions can be formed by photoionization.

Abstract: A method is provided increasing the useful dynamic range of an ion mobility spectrometry (IMS) or an IMS-mass spectrometry (IMS-MS) device. The method includes accumulating a first sample of ions over a first time interval; providing the first sample of ions to an ion detector to provide a first frame, accumulating a second sample of ions over a second time interval, where the second time interval is different than the first time interval, and providing the second sample of ions to the ion detector to provide a second frame. First data points of the first frame are selectively combined with second data points of the second frame to provide an accumulation frame of the first and second samples of ions.

Abstract: Methods and analysers useful for time of flight mass spectrometry are provided.

Abstract: A mass spectrum is acquired by accumulating parent ions in an ion trap, ejecting parent ions of a selected m/z ratio into a collision cell, producing fragment ions from the parent ions, and analyzing the fragment ions in a mass analyzer. The other parent ions remain stored in the ion trap, and thus the process may be repeated by mass-selectively scanning parent ions from the ion trap. In this manner, the full mass range of parent ions or any desired subset of the full mass range may be analyzed without significant ion loss or undue time expenditure. The collision cell may provide a large ion acceptance aperture and relatively smaller ion emission aperture. The collision cell may pulse ions out to the mass analyzer. The mass analyzer may be a time-of-flight analyzer. The timing of pulsing of ions out from the collision cell may be matched with the timing of pulsing of ions into the time-of-flight analyzer.

Abstract: All data obtained from an A/D converter during a summation period with values exceeding a prescribed threshold is excluded from summation up to a prescribed maximum number of deletions, and the values of the remaining data are summed to find one piece of measurement data corresponding to that summation period. The number of pieces of noise data reflecting the influx of particles that cause spike-like noise is extremely small, so noise data is removed in a state in which there are no signals and no target ions are present. When target ions are present, legitimate data which is not noise data may be removed, but the number of pieces of removed data is small in comparison to the total number of pieces of data, so the effects of the removal of legitimate data essentially do not emerge in the summation results. It is therefore possible to effectively reduce spike-like noise.

Abstract: Methods, apparatus and systems for acquiring spectrometric data from analyte ions implement a combination of drift-type ion mobility (IM) separation and time-of-flight mass spectrometry (TOF MS). Both separation techniques are carried out in tandem while applying mass filtering with a wide window of ion isolation. One mode of operation entails utilizing a mass filter to limit ion packets to ions in a selected m/z range that remains constant over the entire course of data acquisition. Another mode entails utilizing the mass filter to limit ion packets to an m/z range that varies over the course of data acquisition.

Abstract: An ion mobility spectrometer comprises a drift tube defining a drift tube inlet configured to receive ions and a drift tube outlet. The drift tube is configured to separate ions in time as a function of ion mobility. The drift tube defines a first ion activation region between the drift tube inlet and the drift tube outlet. The first ion activation region is configured to selectively induce structural changes in at least some of the ions.

Abstract: A method creates an accurate mass spectrum with a high resolving power based on a plurality of TOF spectra, while reducing the computation to assure real-time processing. TOF spectra are measured when ions are ejected from the loop orbit. Then a coincidence detection method determines what mass-to-charge ratio a peak appearing on the TOF spectra originates from. The time range in which a corresponding peak appears on other TOF spectra is set, and the existence of the peak in that range is determined. When the corresponding peak is found on other TOF spectra, the m/z is deduced from the peak on the TOF spectrum with the highest resolving power and a mass spectrum is created. From the peak density around the peak of interest, the reliability of the deduction is computed. For a low reliability peak, the ion ejection time is optimized and the TOF spectrum is measured again.

Abstract: Silver nanoparticles as a sample matrix for Matrix Assisted Laser Desorption Ionization (MALDI) along with a novel method for nanoparticle development is described herein. The silver nanoparticles were generated from silver ions on the surface of a MALDI plate utilizing a Soft Landing Ion Mobility (SLIM) instrument. Upon interaction with the surface the incident silver ions were labile and aggregated into the nanoparticle structures in a time dependent fashion. Post landing analysis were completed by Time of Flight mass spectrometry (TOF), and of particular interest in the spectra were the elimination of low mass interference peaks that generally plague organic based matrices. The approach of the present invention significantly decreases sample preparation time and may lead to a preparation free MALDI source by soft landing a matrix directly on the sample surface.

Abstract: Microchips which are particularly suitable for use with a mass spectrometer include a microchip body with at least one fluid channel formed into the microchip body and at least two flat monolithic closely spaced integrated ESI (electrospray ionization) emitters defined by shaped projections formed to extend from one side of the microchip body, a respective one being in fluid communication with a fluid channel. Related systems and methods are also described.

Abstract: The subject of the invention is a method for identification of analytes with an ion mobility spectrometer by performing a series of measurements while varying the residence time of the analytes in the reaction space and identifying of monomer and nascent dimer analytes in the spectra so obtained.

Abstract: The present invention relates to the analytical electronics used to identify compositions and structures of substances, in particular, to the analyzers comprising at least one mass-spectrometer (MS) and may be applied in such fields as medicine, biology, gas and oil industry, metallurgy, energy, geochemistry, hydrology, ecology. Technical result provides the increase in MS resolution, gain in sensitivity, precision and measurement rates of substances compositions and structures concurrently with enhancement of analyzer functional capabilities, downsizing and mass reduction. In claimed invention the ion flux generation and its guiding are performed in off-axis single-flow mode; parallel multi-stage mode; through use of three-dimensional field with mean meridian surface including without limitation three-dimensional reflecting and dual-zoned reflecting modes or by method of multi-reflection arrays. Devices to implement the claimed method are embodied.

Abstract: The invention generally relates to systems and methods for transferring ions for analysis. In certain embodiments, the invention provides a system for analyzing a sample including an ionizing source for converting molecules of a sample into gas phase ions in a region at about atmospheric pressure, an ion analysis device, and an ion transfer member operably coupled to a gas flow generating device, in which the gas flow generating device produces a laminar gas flow that transfers the gas phase ions through the ion transfer member to an inlet of the ion analysis device.

Abstract: The invention relates generally to ion mobility based systems, methods and devices for analyzing samples and, more particularly, to sample pre-separation and amplification.