Abstract: CL-1577-B.sub.4 compound is derived by the solvolysis under anhydrous conditions of either CL-1577A compound of CL-1577B compound, obtained from the fermentative action of a purified strain of Actinomycete designated ATCC 39363 under aerobic conditions.CL-1577-B.sub.4 compound is a potent antimicrobial agent, possessing activity against a number of gram-positive and gram-negative bacteria, yeasts, and fungi. In addition, the compound exhibits in vivo activity against the P388 murine leukemia cell line and in vitro activity against the L1210 murine leukemia cell line.A method of making the compound as well as pharmaceutical compositions and methods for their use are also disclosed.

Abstract: A method for extracting large quantities of an active biological factor that restores contact inhibition of growth to malignant cell types of different mammalian species.

Abstract: A novel proteinous substance KUP-PC, which is produced by culturing proteinous substance KUD-PC producing organisms belonging to the genus Streptosporangium. KUD-PC increases the antitumor activity of the anti-tumor substance, sporamycin.

Abstract: The invention is a high potency mycelial-free avoparcin alkyl sulfate complex essentially free of dark colored organic derivatives formed in the fermentative biosynthesis of said antibiotic and a process for the preparation thereof.

Abstract: A piece of internal tissue of the sclerotium of Poria cocos (Fr.) Wolf is transplanted into an artificial culture medium to subject it to pure culture of hyphae of said microorganism, thereby obtaining mycelia, and the thus obtained mycelia are extracted with at least one medium selected from the group consisting of water and water-soluble organic media. The resultant substance has an excellent ani-tumor activity and is low in toxicity.

Abstract: Disclosed and claimed is an improved fermentation process for preparing the known antibiotic U-43,120, herein referred to as paulomycin. Also disclosed and claimed are the novel and useful antibiotics paulomycin A and paulomycin B.

Abstract: A process for production of an anti-tumor agent which comprises subjecting the culture cells of the strains belonging to Streptococcus pyogenes in sequence to pasteurization treatment (the first step) and penicillin treatment (the second step), followed by a primary lyophilization (the third step) to obtain an intermediary preparation, and then subjecting the intermediary preparation to penicillin removal treatment (the fourth step), followed by a secondary lyophilization (the fifth step).

Abstract: Described herein is a method for the extraction of an active biochemical factor that restores contact inhibition of growth to malignant cell types of different mammalian species.

Abstract: A process for isolating ergot alkaloids from culture suspensions, in which after mechanical filtration, the residue is dried in a fluidized drying bed and the alkaloids are extracted with an aprotic organic solvent, reextracted with a weakly acidic aqueous solution which is subsequently made basic, and finally extracted with a water-immiscible organic solvent, from which the alkaloids are isolated in a conventional manner. In certain instances, the culture suspension is stirred with an adsorbent clay to entrain the water-soluble alkaloids.

Abstract: Process for the purification of L-(.alpha.S,5S)-.alpha.-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid (AT-125). Also provided are AT-125 analogs prepared in the process.

Abstract: The antibacterial compound S551-II (Reductiomycin) is produced by culturing a microorganism belonging to the genus Streptomyces.S551-II-A is prepared by sublimation of the compound S551-II.

Abstract: A method is provided for recovering a polyether antibiotic, such as lonomycin, monensin or ionomycin from a fermentation broth, wherein the polyether is first extracted with a water-immiscible solvent, such as ethyl acetate, and the resulting extracts concentrated, dissolved in aqueous methanol and then extracted into hexane, benzene or mixtures thereof. The polyether is thereby recovered in crystalline form directly from the latter extract.

Abstract: Fermentation broths or impure solutions containing thienamycin, a substance having antibiotic activity against gram-negative and gram-positive microorganisms, are purified using a sequence of ion exchange resins, starting with an anion exchange resin of the polystyrene-trimethylammonium type in the HCO.sub.3 -cycle, and ending with absorption on and elution from a polymeric cross-linked polystyrene-type resin adsorbent. Variations in the intermediate steps, e.g. different resins and eluates are possible and are illustrated within.

Abstract: Crude, partially purified or contaminated nystatin is purified by suspending the nystatin starting material in water; extracting its activity with sodium lauryl sulfate; adding a chlorinated hydrocarbon solvent; and adding an acid-addition salt of an amine to yield an amorphous precipitate of the nystatin.

Abstract: Acetone or methanol saturated with sodium iodide or sodium thiocyanate are useful solubilizing media for amphotericin B.

Abstract: A new antibiotic SF-1771 substance is produced by cultivating a new strain, Streptomyces toyocaensis SF-1771, in a liquid culture medium under aerobic conditions. This antibiotic may be isolated from the fermentation broth by treating the broth filtrate with a synthetic adsorbent resin for adsorption of the active compound and eluting the resin with an aqueous alcohol or aqueous acetone, followed by chromatographic purification. SF-1771 substance is a copper-containing antibiotic which shows antibacterial activity against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Removal of the copper component from SF-1771 substance by treatment with hydrogen sulfide, an alkali metal sulfide or a copper-chelating agent gives SF-1771-B substance containing no copper component which shows antibacterial activity as high as but a toxicity lower than SF-1771 substance.

Abstract: There is provided a process for recovering macromomycin (hereinafter referred to as MCR) which comprises adding coagulants or coagulants and neutralizers to a culture filtrate saturated with ammonium sulfate and collecting the resulting precipitate containing MCR in a high yield at low centrifugal force. From the precipitate obtained, MCR powders can be prepared according to the methods described in U.S. Pat. No. 3,595,954 and others.

Abstract: A novel antibiotic designated herein MM 17880 may be obtained from the cultivation of strains of Streptomyces olivaceus and related organisms. In addition to being potent antibacterial agents, MM 17880 and its salts inhibit .beta.-lactamase obtained from may organisms so that it shows a synergistic antibacterial effect when combined with .beta.-lactam antibiotics.

Abstract: A new methyl pentaene, lymphosarcin, is provided by the culturing of a new strain of Streptomyces murinus. Lymphosarcin exhibits antineoplastic activity.

Abstract: There is provided a process for producing a stable macromomycin (hereinafter often referred to as MCR) powder which comprises adding a stablilizer selected from the group consisting of saccharides, amino acids and salts thereof, organic acid salts, inorganic acid salts and a chelating agent to a solution of macromomycin purified from fermentation broth prior to dehydration as by lyophilization.

Abstract: The invention provides a novel preparation, preferably in ointment form, for local treatment of acne vulgaris, methods for production of the same and method for treatment of acne vulgaris therewith.

Abstract: Mycoheptin, an antifungal antibiotic, is obtained by extracting the antibiotic from the mycelium with a 65 to 80-percent aqueous solution of isopropanol which contains from 1 to 10 percent by weight of calcium chloride and 10 to 15 percent by weight of methylene chloride, precipitating the antibiotic, washing it with aqueous isopropanol, reprecipitating it from a dimethylformamide solution with water, and treating the product with a 20 to 50-percent aqueous solution of n-propanol containing from 0.2 to 0.5 percent by weight of sodium hexametaphosphate.The novel extracting and reprecipitating agents employed in the process of this invention result in a yield which is 1.5-times that of known methods of mycoheptine recovery from the mycelium as well as in a higher quality of the desired product.

Abstract: The present invention is concerned with a new and improved process for the recovery of the polyene antibiotic partricin, wherein a surface-active agent is added to a fermented liquor containing partricin in an amount sufficient to bring the partricin into solution, whereafter insoluble material is removed and the partricin is recovered from the aqueous solution.

Abstract: There is disclosed a process for obtaining acetylated watersoluble extracts of corynebacteria and specifically of Corynebacterium parvum, of low molecular weight. Said process comprises acetylating delipidated bacterial residues of bacteria cells and isolating the watersoluble extracts by physico-chemical means. Said extracts are useful as immunological adjuvants in veterinary and human therapy.

Abstract: Watersoluble extracts of corynebacteria cells are obtained by a process which comprises the steps of subjecting bacterial residues of corynebacteria containing diaminopimelic acid, e.g. Corynebacterium parvum cells, to a mild extraction and of isolating the watersoluble extracts so obtained by physico-chemical methods. Said extracts are useful as immunological adjuvants.

Abstract: Recovery of an antibiotic selected from the group consisting of antibiotic A-28086 factor A, antibiotic A-28086 factor B, antibiotic A-28086 factor D, and salinomycin by acidifying the fermentation broth in which said antibiotic is produced to about pH 2.0-4.0 and separating the antibiotic with the culture solids.

Abstract: Crude, partially purified, or contaminated nystatin is purified by suspending the nystatin starting material in a lower alkanol, treating the suspension with an organic acid, filtering the suspension to remove residual solids, adding a chlorinated hydrocarbon solvent to the alkanol filtrate, neutralizing the mixture, and precipitating the nystatin by the addition of water.

Abstract: Alkanoyl-josamycins having no bitter taste, in which at least one of the two secondary hydroxyl groups of Josamycin is acylated (introduction of alkanoyl group).

Abstract: This disclosure describes a reversible complex of antibiotic AV290 with an alkali metal alkyl sulfate and a process for preparing same. The complex is useful as an animal feed supplement which significantly enhances the growth rate of animals and poultry.

Abstract: The specification discloses a process for preparing cirramycin A.sub.1 which comprises fermentation of cirramycin B.sub.1 and subsequent conversion thereof to cirramycin A.sub.1 by incubation in the presence of cane molasses.

Abstract: Composition and use of .gamma.-linolenic acid and related materails with ascorbic acid, or ethyl alcohol, or an opiate antagonist such as naloxone, nalorphine or levallorphan, for treating alcoholism or moderating the effects of taking alcohol.