Proline; Hydroxyproline; Histidine Patents (Class 435/107)
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Patent number: 7300776Abstract: An L-amino acid-producing strain of Escherichia coli is bred by modifying an Escherichia coli K12 strain or a derivative thereof so as to become resistant to L-valine and have an ability to produce one or more L-amino acids selected from the group consisting of L-tryptophan, L-phenylalanine, L-lysine, L-tyrosine, L-glutamic acid, L-histidine, L-cysteine, and L-proline.Type: GrantFiled: April 25, 2005Date of Patent: November 27, 2007Assignee: Ajinomoto Co., Inc.Inventors: Hisao Ito, Yuji Joe
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Patent number: 7300777Abstract: The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.Type: GrantFiled: May 25, 2005Date of Patent: November 27, 2007Assignees: Archer-Daniels-Midland Company, Massachusetts Institute of TechnologyInventors: Paul D. Hanke, Anthony J. Sinskey, Laura B. Willis, Stephane Guillouet
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Patent number: 7238501Abstract: The present invention is directed to a process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. In addition, the present invention is directed to a process for producing trans-4-hydroxy-L-proline, wherein the L-proline biosynthesis activity of the host cell of the transformant is reinforced.Type: GrantFiled: July 2, 2003Date of Patent: July 3, 2007Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Shigeru Chiba
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Patent number: 7160703Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the PtsI protein and a process for the fermentative preparation of amino acids using bacteria in which the ptsI gene is enhanced.Type: GrantFiled: June 13, 2003Date of Patent: January 9, 2007Assignee: Degussa AGInventors: Bettina Moeckel, Stephan Hans, Natalie Schischka, Walter Pfefferle
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Patent number: 7157251Abstract: The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.Type: GrantFiled: September 2, 2003Date of Patent: January 2, 2007Assignee: Degussa AGInventors: Andreas Bommarius, Karlheinz Drauz, Stefan Verseck
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Patent number: 7067289Abstract: The present invention provides a method for producing an amino acid selected from the group consisting of L-alanine, L-valine, L-leucine, L-isoleucine, L-methionine, L-phenylalanine, L-proline, glycine, L-serine, L-threonine, L-cysteine, L-tyrosine, L-asparagine, L-glutamine, L-lysine, L-histidine, L-arginine, L-aspartic acid and L-glutamic acid and useful as medicament, chemical agent, food material and feed additive at high industrial efficiency, the method comprising culturing a microorganism having an ability to produce the amino acid and having resistance to an aminoquinoline derivative in a medium, producing and accumulating the amino acid in the present invention in the culture, and recovering the amino acid from the culture.Type: GrantFiled: September 19, 2000Date of Patent: June 27, 2006Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kuniki Kino, Tetsuya Abe
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Patent number: 7037690Abstract: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.Type: GrantFiled: March 26, 2003Date of Patent: May 2, 2006Assignee: Ajinomoto Co., Inc.Inventors: Yoshihiko Hara, Hiroshi Izui, Takahiro Asano, Yasuyuki Watanabe, Tsuyoshi Nakamatsu
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Patent number: 6984514Abstract: L-Glutamic acid, L-proline or L-arginine is produced by culturing a bacterium belonging to the genus Escherichia, which is L-isoleucine auxotrophic and has ability to produce L-glutamic acid, L-proline or L-arginine, in a medium containing L-isoleucine, to produce and accumulate L-glutamic acid, L-proline or L-arginine in a culture, and collecting L-glutamic acid, L-proline or L-arginine from the culture.Type: GrantFiled: September 17, 2001Date of Patent: January 10, 2006Assignee: Ajinomoto Co., Inc.Inventors: Maria Grigorievna Lunts, Svetlana Aleksandrovna Fomina, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner
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Patent number: 6979560Abstract: A bacterium belonging to the genus Escherichia and having an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing an expression amount of an L-amino acid excretion protein, and a method for producing the L-amino acid using the bacterium.Type: GrantFiled: December 13, 1999Date of Patent: December 27, 2005Assignee: Ajinomoto Co., Inc.Inventors: Vitaliy Arkadievich Livshits, Natalia Pavlovna Zakataeva, Kazuo Nakanishi, Vladimir Veniaminovich Aleshin, Petr Vladimirovich Troshin, Irina Lyvovna Tokhmakova
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Patent number: 6953677Abstract: A novel rec-L-N-carbamoylase from Arthrobacter aurescens and its method of use for producing L-amino acids. The recombinantly produced L-carbamoylase is unexpectedly stable, so that an industrial method of producing L-amino acids can be established with it, in contrast to previously known L-carbamoylases.Type: GrantFiled: January 15, 2002Date of Patent: October 11, 2005Assignees: Degussa AG, Universitat StuttgartInventors: Josef Altenbuchner, Ralf Mattes, Markus Pietzsch, Christoph Syldatk, Anja Wiese, Burkard Wilms
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Patent number: 6808914Abstract: The invention relates to nucleotide sequences coding for the accDA gene and to a process for the preparation of L-amino acids, especially L-lysine, by fermentation using corynebacteria in which the accDA gene is amplified.Type: GrantFiled: December 21, 2001Date of Patent: October 26, 2004Assignee: Degussa AGInventors: Yvonne Tilg, Bernhard Eikmanns, Lothar Eggeling, Hermann Sahm, Bettina Möckel
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Publication number: 20040191876Abstract: L-Glutamic acid, L-proline or L-arginine is produced by culturing a bacterium belonging to the genus Escherichia, which is L-isoleucine auxotrophic and has ability to produce L-glutamic acid, L-proline or L-arginine, in a medium containing L-isoleucine, to produce and accumulate L-glutamic acid, L-proline or L-arginine in a culture, and collecting L-glutamic acid, L-proline or L-arginine from the culture.Type: ApplicationFiled: April 13, 2004Publication date: September 30, 2004Applicant: AJINOMOTO CO., INC.Inventors: Maria Grigorievna Lunts, Svetlana Aleksandrovna Fomina, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner
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Patent number: 6767726Abstract: An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.Type: GrantFiled: May 6, 2002Date of Patent: July 27, 2004Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Patent number: 6706517Abstract: The present invention provides an industrially efficient method for producing an L-amino acid useful as medicament, chemical agent, food material and feed additive, and the method comprising culturing in a medium a microorganism having an ability to produce the L-amino acid and having resistance to a DNA gyrase inhibitor or a microorganism having an ability to produce the L-amino acid and having both resistance to a DNA gyrase inhibitor and resistance to an aminoquinoline derivative, producing and accumulating the L-amino acid therein and recovering the L-amino acid therefrom.Type: GrantFiled: December 21, 2001Date of Patent: March 16, 2004Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kuniki Kino, Tetsuya Abe
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Publication number: 20040048345Abstract: Provided is an industrially applicable process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium.Type: ApplicationFiled: July 2, 2003Publication date: March 11, 2004Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Shigeru Chiba
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Patent number: 6686181Abstract: Process for the preparation of L -amino acids from their racemic N-acetyl-D,L derivatives by enzymatic resolution by means of isolated, recombinant enzymes A process for the preparation of proteinogenic or nonproteinogenic L-amino acids, in particular L-phosphinothricin, from their racemic N-acetyl-D,L derivatives comprises a) selectively deacetylating N-acetyl-L derivatives of the corresponding L-amino acids by an enzymatic resolution by means of isolated, recombinant enzymes, while N-acetyl-D derivatives of the corresponding D-amino acids are not deacetylated and (b) separating the deacetylated L-amino acids obtained preparatively from the nondeacetylated N-acetyl-D derivatives and/or the incompletely deacetylated N-acetyl-L derivatives.Type: GrantFiled: January 17, 2002Date of Patent: February 3, 2004Assignee: Aventis Corporation GmbHInventor: Klaus Bartsch
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Publication number: 20040014180Abstract: The invention relates to a method for the microbial production of metabolic products, polynucleotides from coryne-form bacteria and use thereof. According to the invention, by means of said method and polynucleotides it is possible to influence the synthesis of ATP in a controlled manner and also to control the synthesis of metabolic products. The invention relates to genes from Corynebacterium glutamicum coding for cytochrome aa3 oxidase and the cytochrome bc1 complex. The monocistronic ctaD gene codes for a 65 kDa protein, the primary structure of which displays all the typical properties of the sub-unit I of cytochrome aa3 oxidase. The genes which code for the sub-unit III of the cytochrome aa3 (ctaE) and the three characteristic sub-units of the cytochrome bc1 complex (qcrABC) are arranged in a group with the sequence ctaE-qcrCAB.Type: ApplicationFiled: August 1, 2003Publication date: January 22, 2004Inventors: Michael Bott, Axel Niebisch, Brigitte Bathe, Achim Marx, Thomas Hermann
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Patent number: 6656710Abstract: The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.Type: GrantFiled: October 11, 2001Date of Patent: December 2, 2003Assignee: Degussa AGInventors: Andreas Bommarius, Karlheinz Drauz, Stefan Verseck
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Publication number: 20030219881Abstract: The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.Type: ApplicationFiled: February 5, 2003Publication date: November 27, 2003Applicant: Degussa AGInventors: Bathe Brigitte, Kreutzer Caroline, Mockel Bettina, Thierbach Georg
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Patent number: 6617140Abstract: Provided is an industrially applicable process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel enzyme L-proline-4-hydroxylase which is useful for the process, a gene of L-proline-4-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-4-hydroxylase using the transformant.Type: GrantFiled: April 13, 2001Date of Patent: September 9, 2003Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Shigeru Chiba
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Publication number: 20030092153Abstract: An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.Type: ApplicationFiled: May 6, 2002Publication date: May 15, 2003Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Patent number: 6551803Abstract: A process is described for purifying an amino acid-containing solution by means of electrodialysis, wherein an amino acid-containing solution is employed which is obtained from the fermentation for producing at least one amino acid.Type: GrantFiled: May 1, 2002Date of Patent: April 22, 2003Assignee: BASF AktiengesellschaftInventors: Andreas Fischer, Christoph Martin, Jürgen Müller
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Publication number: 20020151000Abstract: Provided is an industrially applicable process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium.Type: ApplicationFiled: April 13, 2001Publication date: October 17, 2002Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Shigeru Chiba
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Patent number: 6413748Abstract: An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.Type: GrantFiled: December 6, 1999Date of Patent: July 2, 2002Assignee: Kyowa Hakko Kogyo, Co., Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Publication number: 20020058315Abstract: L-Glutamic acid, L-proline or L-arginine is produced by culturing a bacterium belonging to the genus Escherichia, which is L-isoleucine auxotrophic and has ability to produce L-glutamic acid, L-proline or L-arginine, in a medium containing L-isoleucine, to produce and accumulate L-glutamic acid, L-proline or L-arginine in a culture, and collecting L-glutamic acid, L-proline or L-arginine from the culture.Type: ApplicationFiled: September 17, 2001Publication date: May 16, 2002Applicant: AJINOMOTO CO., INC.Inventors: Maria Grigorievna Lunts, Svetlana Aleksandrovna Fomina, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner
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Patent number: 6365381Abstract: The present invention provides a method for industrially advantageously producing (S)-4-hydroxy-2-ketoglutaric acid and for producing compounds which are formed by biosynthesis from the precursor (S)-4-hydroxy-2-ketoglutaric acid, for example, for producing the compounds (2S,4S)-4-hydroxy-L-glutamic acid and (2S,4S)-4-hydroxy-L-proline, using a recombinant microorganism carrying a recombinant DNA harboring the DNA fragment encoding 4(S)-4-hydroxy-2-ketoglutaric acid aldolase gene.Type: GrantFiled: January 19, 2001Date of Patent: April 2, 2002Assignee: Kyowa Hakko Kogyo, Ltd.Inventors: Shin-ichi Hashimoto, Ryoichi Katsumata
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Publication number: 20020037559Abstract: Microorganism which can utilize an N-protected proline derivative of general formula (I) in the form of the racemate or one of its optically active isomers, R1 meaning —(CH2)2—COOH or, optionally substituted in each case, C1-C4 alkoxy, aryl or aryloxy, and R2 meaning hydrogen or hydroxy, as the only nitrogen, only carbon or only carbon and nitrogen source. These microorganisms can be used in a process for producing N-protected cyclic or aliphatic D-amino acid derivatives of general formulae (II) and (V), A together with —N— and —CH— and R3, R4 and R5 having the given meanings.Type: ApplicationFiled: August 25, 1998Publication date: March 28, 2002Inventors: MARTIN SAUTER, DANIEL VENETZ, FABIENNE HENZEN, DIEGO SCHMIDHALTER, GABRIELA PFAFFEN, OLEG WERBITZKY
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Method for optically resolving a racemic alpha-substituted heterocyclic carboxylic acid using enzyme
Publication number: 20020025565Abstract: Disclosed is a method for optically resolving a racemic &agr;-substituted heterocyclic carboxylic acid (&agr;-HCCA) by taking advantage of enantioselectivity of enzymes. &agr;-HCCA is reacted with alcohol to give a racemic &agr;-HCCA ester, which is then reacted with an enzyme with enantioselectivity, whereby either R-form or S-form of the racemate is hydrolyzed. Extraction with an organic solvent can obtain enantiomers of the &agr;-HCCA ester. The extracted enantiomeric &agr;-HCCA ester is converted to enantiomeric &agr;-HCCA by catalytic hydrogenation in an organic solution or by enzymatic hydrolysis in an aqueous solution. Thus, a racemic mixture of &agr;-HCCA can be optically resolved with high optical purity at high yields as well as at low cost.Type: ApplicationFiled: May 31, 2001Publication date: February 28, 2002Inventors: Ki-Nam Uhm, Sang-Chul Lim, Jong-Ho Lim -
Patent number: 6344347Abstract: The present invention provides an industrially efficient method for producing an L-amino acid useful as medicament, chemical agent, food material and feed additive, and the method comprising culturing in a medium a microorganism having an ability to produce the L-amino acid and having resistance to a DNA gyrase inhibitor or a microorganism having an ability to produce the L-amino acid and having both resistance to a DNA gyrase inhibitor and resistance to an aminoquinoline derivative, producing and accumulating the L-amino acid therein and recovering the L-amino acid therefrom.Type: GrantFiled: September 18, 2000Date of Patent: February 5, 2002Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kuniki Kino, Tetsuya Abe
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Publication number: 20020006644Abstract: Disclosed is a method for preparing an R- or S-forms of &agr;-substituted heterocyclic carboxylic acid (&agr;-HCCA) and a counter enantiomeric form of &agr;-substituted heterocyclic carboxylic acid ester thereto by use of an enzyme. A racemic &agr;-HCCA is reacted with alcohol to give a racemic &agr;-HCCA ester, which is then brought into contact with an enzyme with enantioselectivity, whereby either R-form or S-form of the racemate is hydrolyzed. Extraction with an organic solvent can obtain enantiomers of the &agr;-HCCA ester. Thus, a certain enantiomeric form of &agr;-HCCA and a counter enantiomeric form of &agr;-HCCA ester thereto, respectively can be prepared with high optical purity at high yields as well as at low cost.Type: ApplicationFiled: May 30, 2001Publication date: January 17, 2002Inventors: Ki-Nam Uhm, Sang-Chul Lim, Jong-Ho Lim
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Publication number: 20010012898Abstract: The present invention relates to a method for preparing a chiral ester and more particularly, the method for preparing an optically pure chiral ester from an alkenyl ester at a high yield by mixing and reacting:Type: ApplicationFiled: December 1, 2000Publication date: August 9, 2001Inventors: Jai Wook Park, Mahn-Joo Kim, Jeong Hwan Koh, Hyun Min Jung
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Patent number: 6238895Abstract: The use of strains of the genus Microbacterium for the production of organic acids or amino acids by the enzymatic conversion of a fumaric acid to the organic acid or amino acid desired, as well as methods for such use and the conversion solution produced by such use and the methods of the present invention are disclosed. The uses and the methods of the present invention provide the L-isomer form of the desired organic acid or amino acid produced thereby in the absence of the D-isomer form of the desired organic acid or amino acid. Also disclosed are reactants solutions which include the strain of the genus Microbacterium and the L-isomer form of either the organic acid or the amino acid.Type: GrantFiled: December 19, 1996Date of Patent: May 29, 2001Assignee: Genencor International, Inc.Inventor: James M. Miers
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Patent number: 6207427Abstract: The present invention provides a method for industrially advantageously producing (S)-4-hydroxy-2-ketoglutaric acid and for producing compounds which are formed by biosynthesis from the precursor (S)-4-hydroxy-2-ketoglutaric acid, for example, for producing the compounds (2S,4S)-4-hydroxy-L-glutamic acid and (2S,4S)-4-hydroxy-L-proline, using a recombinant microorganism carrying a recombinant DNA harboring the DNA fragment encoding 4(S)-4-hydroxy-2-ketoglutaric acid aldolase gene.Type: GrantFiled: June 5, 1998Date of Patent: March 27, 2001Assignee: Kyowa Hakko Kogyo, Ltd.Inventors: Shin-ichi Hashimoto, Ryoichi Katsumata
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Patent number: 6204050Abstract: The present invention relates to two novel microorganisms, Acinetobacter baumanni WT-50/50-4A (ATCC 202144) and Serratia marcescens WT-L-4A1(RED) (ATCC 202145) characterized by 4-hydroxyproline epimerase activity. The compounds trans-4-hydroxy-L-proline (THLP) or cis-4-hydroxy-D-proline (CHDP) are converted with this epimerase activity to a mixture of THLP and CHDP. Additionally, the compounds trans-4-hydroxy-D-proline (THDP) or cis-4-hydroxy-L-proline (CHLP) are converted with this epimerase activity to a mixture of THDP and CHLP.Type: GrantFiled: July 19, 1999Date of Patent: March 20, 2001Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert Dicosimo, Susan K. Fager, John E. Gavagan
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Patent number: 6171829Abstract: A substance PF1191 having an inhibitory activity to kainic acid toxicity represented by the following formula (I) which is obtained by incubating a fungus belonging to the genus Eupenicillium and isolating the product thus produced from the culture by solvent extraction, adsorption column chromatography, gel filtration, etc.Type: GrantFiled: September 10, 1999Date of Patent: January 9, 2001Assignee: Meiji Seika Kaisha, Ltd.Inventors: Haruo Seto, Kazuo Shin-Ya, Takashi Yaguchi, Toru Sasaki
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Patent number: 6143552Abstract: The present invention provides a process for producing an L-amino acid which comprises culturing in a nutrient medium a microorganism which is capable of producing the L-amino acid and which can not grow in a synthetic medium containing said L-amino acid as the sole nitrogen source in an amount of 5 mg/ml or below, allowing the L-amino acid to accumulate in the culture, and recovering the L-amino acid from the culture.Type: GrantFiled: March 11, 1999Date of Patent: November 7, 2000Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kazuyuki Okamoto, Masato Ikeda, Kuniki Kino
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Patent number: 6133018Abstract: 2-Aminopropane is used as the amine donor in the stereoselective synthesis of a chiral amine from a ketone with a transaminase. In a typical embodiment, (S)-1-methoxy-2-aminopropane is prepared by bringing methoxyacetone into contact with a transaminase in the presence of 2-aminopropane as an amine donor until a substantial amount of methoxyacetone is converted to (S)-1-methoxy-2-aminopropane and 2-aminopropane is converted to acetone. In a second embodiment, L-alanine is prepared by bringing pyruvic acid into contact with a transaminase in the presence of 2-aminopropane as an amine donor.Type: GrantFiled: March 10, 1999Date of Patent: October 17, 2000Assignee: CelgroInventors: Wei Wu, Mohit B. Bhatia, Craig M. Lewis, Wei Lang, Alice L. Wang, George W. Matcham
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Patent number: 6083917Abstract: Disclosed are methods and compositions for the identification, characterization and inhibition of mammalian protein farnesyltransferases, enzymes involved in the farnesylation of various cellular proteins, including cancer related ras proteins such as p21.sup.ras. One protein farnesyltransferase which is disclosed herein exhibits a molecular weight of between about 70,000 and about 100,000 upon gel exclusion chromatography. Also disclosed are methods and compositions for the preparation of farnesyltransferase by recombinant means, following the molecular cloning and co-expression of its two subunits, for assay and purification of the enzyme, as well as procedures for using the purified enzyme in screening protocols for the identification of possible anticancer agents which inhibit the enzyme and thereby prevent expression of proteins such as p21.sup.ras.Type: GrantFiled: August 24, 1992Date of Patent: July 4, 2000Assignee: Board of Regents, The University of Texas SystemInventors: Michael S. Brown, Joseph L. Goldstein, Yuval Reiss, Jim Marsters
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Patent number: 6063615Abstract: Disclosed is a process for preparing acylated amino esters and a process for preparing optically active amino esters from racemic amino esters with a carboxylic ester as acylating agent, whose acid component has a halogen, nitrogen, oxygen or sulfur atom neighboring the carbonyl carbon atom, in the presence of a hydrolase selected from the group of amidase, protease, esterase and lipase, and subsequent separation of the enantioselectively acylated amino ester from the non-acylated other enantiomer of the amino ester.Type: GrantFiled: June 25, 1998Date of Patent: May 16, 2000Assignee: BASF AktiengesellschaftInventors: Rainer Sturmer, Klaus Ditrich, Wolfgang Siegel
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Patent number: 6010891Abstract: An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant, is also provided.Type: GrantFiled: April 7, 1999Date of Patent: January 4, 2000Assignee: Kyowa Hakko Kogyo, Co, Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Patent number: 5962292Abstract: An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.Type: GrantFiled: September 9, 1996Date of Patent: October 5, 1999Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Patent number: 5928933Abstract: The present invention relates to a process for the enzymatic resolution of racemic mixtures of N-(alkoxycarbonyl)-4-keto-D,L-proline alkyl esters, using Candida antarctica lipase fraction B (CALB) as enzyme catalyst to enantioselectively hydrolyze the alkyl ester of one of the two enantiomers present. Separating the unreacted N-(alkoxycarbonyl)-4-keto-D-proline alkyl ester from the N-(alkoxycarbonyl)-4-keto-L-proline, followed by hydrogenation of the keto group of the D-isomer and subsequent hydrolysis of the ester and N-(alkoxy-carbonyl) groups produces cis-4-hydroxy-D-proline in high yield. Diastereomeric mixtures of N-(alkoxycarbonyl)-4-hydroxyproline alkyl esters can also be resolved using CALB to ultimately produce cis-4-hydroxy-D-proline or trans-4-hydroxy-L-proline.Type: GrantFiled: June 26, 1998Date of Patent: July 27, 1999Assignee: E. I. du Pont de Nemours & CompanyInventors: Robert Dicosimo, Wonpyo Hong
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Patent number: 5919670Abstract: The present invention provides a process for producing an L-amino acid which comprises culturing in a nutrient medium a microorganism which is capable of producing the L-amino acid and which can not grow in a synthetic medium containing said L-amino acid as the sole nitrogen source in an amount of 5 mg/ml or below, allowing the L-amino acid to accumulate in the culture, and recovering the L-amino acid from the culture.Type: GrantFiled: June 18, 1998Date of Patent: July 6, 1999Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kazuyuki Okamoto, Masato Ikeda, Kuniki Kino
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Patent number: 5876983Abstract: A phosphoenolpyruvate carboxylase gene, which has mutation such as mutation to replace 625th glutamic acid from the N-terminus of phosphoenolpyruvate carboxylase with lysine, mutation to replace 438th arginine from the N-terminus with cysteine and the like, is introduced into Escherichia coli or coryneform bacteria, so as to produce a phosphoenolpyruvate carboxylase which is not substantially inhibited by aspartic acid, thereby amino acid is efficiently produced.Type: GrantFiled: April 29, 1996Date of Patent: March 2, 1999Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugimoto, Tomoko Suzuki, Hiroshi Matsui, Katsura Izui
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Patent number: 5854040Abstract: Isolated and purified L-proline-4-hydroxylase which catalyzes hydroxylation of L-proline at the 4-position of L-proline in the presence of 2-ketoglutaric acid and a divalent iron ion to produce trans-4-hydroxy-L-proline. The L-proline-4-hydroxylase may be isolated from a microorganism selected from the group consisting of Dactylosporanlium sp. RHI deposited with the National Institute of Bioscience and Human-Technology Agency of Industrial Science and Technology, Japan, under the designation PERM BP-4400 and Amycolatopsis sp. RH2 deposited as FERM BP-4581.Type: GrantFiled: September 9, 1996Date of Patent: December 29, 1998Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Shigeru Chiba
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Patent number: 5783427Abstract: Disclosed is a process for preparing a D-amino acid selected from the group consisting of D-methionine, D-valine, D-leucine, D-isoleucine and D-histidine, which comprises the steps of:making a culture or treated culture of a microorganism having ability to asymmetrically degrade a L-amino acid selected from the group consisting of L-methionine, L-valine, L-leucine, L-isoleucine and L-histidine act on a corresponding racemic amino acid to the L-amino acid; andseparating and collecting the remaining D-amino acid.Type: GrantFiled: March 26, 1996Date of Patent: July 21, 1998Assignee: Tanabe Seiyaku Co., Ltd.Inventors: Masakatsu Furui, Eiji Takahashi, Hiroyasu Seko, Takeji Shibatani
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Patent number: 5773321Abstract: A method of counting semiconductor integrated circuit devices comprising steps of forming a semiconductor device block having a plurality of semiconductor integrated circuit devices of vertical mounting type coupled to each other in parallel, and mounting the semiconductor device block on a printed board. A block of semiconductor integrated circuit devices comprising a plurality of semiconductor integrated circuit devices of vertical mounting type, and coupling means for coupling the plurality of semiconductor integrated circuit devices each other in parallel.Type: GrantFiled: December 13, 1996Date of Patent: June 30, 1998Assignee: Kabushiki Kaisha ToshibaInventors: Toshimitsu Ishikawa, Atsushi Kitamura, Kenji Hirayama
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Patent number: 5728555Abstract: The present invention relates to materials and methods for production of natural and unnatural D-amino acids. In particular, the present invention relates to a fermentation method for the production of D-amino acids using recombinant host cells.Specifically, the invention relates to a method for producing a D-amino acid in a cell, comprising:(a) incorporating into the cell a D-aminotransferase gene and a L-aminodeaminase gene;(b) culturing the cell in a cell culture medium; and(c) isolating the D-amino acid from the cell culture medium.The invention also relates to a method for producing D-phenylalanine in a cell, comprising:(a) incorporating into the cell a D-aminotransferase gene, a L-aminodeaminase gene and means for increasing production of phenylpyruvate;(b) culturing the cell in a cell culture medium; and(c) isolating the D-phenylalanine from the cell culture medium.The invention also relates to the preparation of recombinant cells for use in the production of enantiomerically pure D-amino acids.Type: GrantFiled: September 30, 1996Date of Patent: March 17, 1998Assignee: Monsanto CompanyInventors: Ian G. Fotheringham, Paul P. Taylor, Jennifer L. Ton
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Patent number: 5714355Abstract: DSM 9771 is a mutant of DSM 7330 which was obtained under selective pressure. Its enzymatic activity is higher by a factor of 2.3 than that of its parent organism. In the presence of an inducer, this activity may be farther increased by a factor of 2.7. The reaction catalyzed by this microorganism or enzymes therefrom is the enantioselective conversion of a D-5-monosubstituted hydantoin or an L-5-monosubstituted hydantoin or a D-N-carbamoyl amino acid or an L-N-carbamoyl amino acid to a corresponding L-.alpha.-amino acid.Type: GrantFiled: May 29, 1996Date of Patent: February 3, 1998Assignee: Degussa AktiengesellschaftInventors: Fritz Wagner, Britta Hantke, Thomas Wagner, Karlheinz Drauz, Andreas Bommarius
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Patent number: 5705370Abstract: Culturing an L-amino acid producing microorganism belonging to the genus Brevibacterium or Corynebacterium and having a resistance to a peptide containing glutamic acid or aspartic acid gives L-amino acids in high yield.Type: GrantFiled: May 12, 1995Date of Patent: January 6, 1998Assignee: Ajinomoto Company, Inc.Inventors: Takayasu Tsuchida, Haruo Uchibori, Hiroshi Takeuchi, Mitsuyoshi Seki