Tryptophan; Tyrosine; Phenylalanine; 3,4 Dihydroxyphenylalanine Patents (Class 435/108)
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Patent number: 11866749Abstract: Described herein are recombinant microbial host cells comprising biosynthetic pathways and their use in producing oxidation products and downstream products, e.g., melatonin and related compounds, as well as enzyme variants, nucleic acids, vectors and methods useful for preparing and using such cells. In specific aspects, the present invention relates to monooxygenases, e.g., amino acid hydroxylases, with a modified cofactor-dependency, and to enzyme variants and microbial cells providing for an improved supply of cofactors.Type: GrantFiled: October 23, 2020Date of Patent: January 9, 2024Assignee: Danmarks Tekniske UniversitetInventors: Hao Luo, Jochen Förster
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Patent number: 11746345Abstract: The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.Type: GrantFiled: May 26, 2016Date of Patent: September 5, 2023Assignee: AJINOMOTO CO., INC.Inventors: Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova
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Patent number: 11198861Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. Methods for producing PAL enzymes are also provided. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activities that are useful under industrial process conditions for the production of pharmaceutical compounds.Type: GrantFiled: June 14, 2019Date of Patent: December 14, 2021Assignee: Codexis, Inc.Inventors: Scott J. Novick, Ravi David Garcia, Charlene Ching, Nikki Dellas, David Entwistle, Vesna Mitchell
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Patent number: 11142780Abstract: Disclosed herein are methods that may be used for the synthesis of benzylisoquinoline alkaloids (BIAs) such as alkaloid morphinan. The methods disclosed can be used to produce thebaine, oripavine, codeine, morphine, oxycodone, hydrocodone, oxymorphone, hydromorphone, naltrexone, naloxone, hydroxycodeinone, neopinone, and/or buprenorphine. Compositions and organisms useful for the synthesis of BIAs, including thebaine synthesis polypeptides, purine permeases, and polynucleotides encoding the same, are provided.Type: GrantFiled: June 27, 2017Date of Patent: October 12, 2021Assignee: Antheia, Inc.Inventors: Peter James Facchini, Xue Chen, Jeffrey C. Colbeck, Joseph Tucker
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Patent number: 11098288Abstract: An enzyme having the following characteristics (a) and (b): (a) the enzyme has an activity of reversible dehydrogenation of D-amino acids; (b) the enzyme is a hexamer of polypeptides having an amino acid sequence having 80% or greater identity to the amino acid sequence of SEQ ID NO: 2.Type: GrantFiled: August 9, 2018Date of Patent: August 24, 2021Assignees: KUREHA CORPORATION, NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Hironaga Akita, Yuusuke Nakamichi, Masahiro Watanabe, Akinori Matsushika, Tomotake Morita
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Patent number: 11066637Abstract: A live bacteria-containing particulate is provided, wherein the particulate comprises a bacterial strain, a first covering layer, and a second covering layer, and wherein the first covering layer is in-between the cell membrane and cell wall of the bacterial strain and the bacterial strain is dispersed in the second covering layer. A method of preparing a live bacteria-containing particulates is also provided, wherein the method comprises the following steps: (a) subjecting a bacterial strain to a three-stage fermentation to obtain a fermentation broth; (b) concentrating the fermentation broth to provide a concentrated bacterial solution; (c) mixing the concentrated bacterial solution with a protective agent; and (d) drying the mixture to provide live bacteria-containing particulates.Type: GrantFiled: March 23, 2020Date of Patent: July 20, 2021Assignee: TCI CO., LTD.Inventors: Yung-Hsiang Lin, Cheng-Yu Ho, Jin-Jia Wang
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Patent number: 11041159Abstract: The present disclosure provides nucleic acids and vectors for use with methanotrophic bacteria. Related host cells and methods for using such nucleic acids and vectors for expressing polypeptides or other genetic manipulation of methanotrophic bacteria are also provided.Type: GrantFiled: August 28, 2019Date of Patent: June 22, 2021Assignee: Calysta, Inc.Inventors: Renee M. Saville, Joshua Silverman, Jeremy Minshull, Jon Edward Ness, Effendi Leonard, Jana Stumpe, Mark Welch
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Patent number: 10815510Abstract: The present application relates to a microorganism of the genus Escherichia producing L-tryptophan and, more specifically, to a microorganism of the genus Escherichia with improved activity of producing L-tryptophan by weakening or inactivating the activity of endogenous 6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase. Additionally, the present application relates to a method for producing L-tryptophan using the microorganism of the genus Escherichia.Type: GrantFiled: June 23, 2015Date of Patent: October 27, 2020Assignee: CJ CHEILJEDANG CORPORATIONInventors: Ki Yong Cheong, Kyung Rim Kim, Seok Myung Lee, Kwang Ho Lee, Keun Cheol Lee, Hyeong Pyo Hong
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Patent number: 10731187Abstract: The invention provides a recombinant microbial host cell capable of converting a raw material comprising a fermentable carbon substrate to o-aminobenzoate biologically. The invention further provides a method for producing aniline, comprising the steps of: a) producing o-aminobenzoate by fermentation of a raw material comprising at least one fermentable carbon substrate using the recombinant microbial host cell of the capable of converting said raw material comprising at least one fermentable carbon substrate to o-aminobenzoate biologically, wherein said o-aminobenzoate comprises anthranilate anion, b) converting said o-aminobenzoate from said anthranilate anion to anthranilic acid by acid protonation, c) recovering said anthranilic acid by precipitation or by dissolving in an organic solvent, and d) converting said anthranilic acid to aniline by thermal decarboxylation in an organic solvent.Type: GrantFiled: February 19, 2015Date of Patent: August 4, 2020Assignee: Covestro Deutschland AGInventors: Gernot Jaeger, Jorgen Magnus, Amgad Salah Moussa, Guenter Olf, Giulio Lolli, Swantje Behnken, Jung-Won Youn, Mohammad Yalfani, Georg Sprenger
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Patent number: 10612056Abstract: The present disclosure provides methods for preparing ?-substituted tryptophan compounds. The methods include: combining i) an unsubstituted indole or a substituted indole, ii) a ?-substituted serine, and iii) a tryptophan synthase ?-subunit (i.e., a TrpB); and maintaining the resulting mixture under conditions sufficient to form the ?-substituted tryptophan. The TrpB contains at least one amino acid mutation which promotes formation of an amino-acrylate intermediate. New TrpB variants and new ?-substituted tryptophan analogs are also described.Type: GrantFiled: May 3, 2018Date of Patent: April 7, 2020Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Christina E. Boville, Sabine Brinkmann-Chen, Andrew R. Buller, David K. Romney, Christopher K. Prier, Philipp Koch, Remkes A. Scheele
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Patent number: 10570422Abstract: A method for the preparation of 2,4-dihydroxybutyric acid from homoserine includes a first step of conversion of the primary amino group of homoserine to a carbonyl group to obtain 2-oxo-4-hydroxybutyrate, and a second step of reduction of the obtained 2-oxo-4-hydroxybutyrate (OHB) to 2,4-dihydroxybutyrate.Type: GrantFiled: July 10, 2013Date of Patent: February 25, 2020Assignee: ADISSEO FRANCE S.A.S.Inventors: Thomas Walther, Hélène Cordier, Clémentine Dressaire, Jean Marie Francois, Robert Huet
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Patent number: 10392672Abstract: The present invention relates to a strain of Rhodotorula sp. OMK-1(CCTCC M 2015326) and a method for producing natural cinnamic acid through the fermentation of Rhodotorula sp. OMK-1. The production method comprises steps of strain activation, seed culture, fermentation, extraction, etc. The fermentation process uses natural glucose, amino acids, etc. as raw materials to produce cinnamic acid via the metabolism of Rhodotorula sp. OMK-1. The present invention provides a safe and environmentally friendly method for producing natural cinnamic acid at low temperature and low pressure with simple operation and reduced pollution.Type: GrantFiled: January 13, 2016Date of Patent: August 27, 2019Assignee: XIAMEN OAMIC BIOTECHNOLOGY CO., LTD.Inventors: Chenguang Xing, Xijing Zhao, Zhiqiang Huang, Cuiping Chen, Wei Liu
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Patent number: 10253307Abstract: Disclosed are a structure and a method capable of protecting from outside stimuli while containing in a liquid state a water-dispersible substance to be protected. The protective structure of the substance to be protected is a water-in-oil emulsion structure comprising an aqueous phase configuring a discontinuous phase containing the water-dispersible substance to be protected, an oil phase in which said aqueous phase is dispersed, and either vesicles formed with an amphiphilic substance which spontaneously forms vesicles or polycondensation polymer particles having hydroxyl groups.Type: GrantFiled: June 17, 2013Date of Patent: April 9, 2019Assignee: KANAGAWA UNIVERSITYInventors: Kazuo Tajima, Yoko Imai
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Patent number: 10174303Abstract: The invention provides genetically engineered microorganisms and methods for producing chorismate-derived products, such as para-hydroxybenzoic acid, salicylate, 2-aminobenzoate, 2,3-dihydroxybenzoate, and 4-hydroxycyclohexane carboxylic acid. Typically, the microorganism comprises at least one of (a) an exogenous chorismate pyruvate lyase, (b) an exogenous isochorismate synthase, (c) an exogenous isochorismate pyruvate lyase, and (d) a prephenate synthase comprising a disruptive mutation.Type: GrantFiled: May 26, 2016Date of Patent: January 8, 2019Assignee: LANZATECH NEW ZEALAND LIMITEDInventors: James Bruce Yarnton Haycock Behrendorff, Michael Koepke, Loan Phuong Tran, Wyatt Eric Allen
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Patent number: 10167477Abstract: A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline.Type: GrantFiled: March 7, 2018Date of Patent: January 1, 2019Assignee: GENOMATICA, INC.Inventor: Priti Pharkya
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Patent number: 10119155Abstract: Methods that may be used for the manufacture of the chemical compound (S)-norcoclaurine, (S)-norlaudanosoline, and (S)-norcoclaurine or [S]-norlaudanosoline synthesis intermediates are provided. (S)-Norcoclaurine, (S)-norlaudanosoline, and (S)-norcoclaurine or (S)-norlaudanosoline synthesis intermediates are useful as precursor products in the manufacture of certain medicinal agents.Type: GrantFiled: June 12, 2015Date of Patent: November 6, 2018Assignee: Epimeron Inc.Inventor: Peter James Facchini
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Patent number: 10023886Abstract: The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c] from the corresponding ketones of the general formula [1][a] by using novel transaminases. These novel transaminases are selected from two different groups: either from a group of some 20 proteins with sequences as specified herein, or from a group of proteins having transaminase activity and isolated from a microorganism selected from the group of organisms consisting of Rahnella aquatilis, Ochrobactrum anthropi, Ochrobactrum tritici, Sinorhizobium morelense, Curtobacterium pusiffium, Paecilomyces lilacinus, Microbacterium ginsengisoli, Microbacterium trichothecenolyticum, Pseudomonas citronellolis, Yersinia kristensenii, Achromobacter spanius, Achromobacter insolitus, Mycobacterium fortuitum, Mycobacterium frederiksbergense, Mycobacterium sacrum, Mycobacterium fluoranthenivorans, Burkhoideria sp.Type: GrantFiled: October 16, 2015Date of Patent: July 17, 2018Assignee: Patheon Holdings I B.V.Inventors: Martin Schurmann, Wijnand Peter Helena Peeters, Natascha Hubertina Johannes Smeets, Helmut Schwab, Kerstin Steiner, Kateryna Mykolayivna Lypetska, Gernot Strohmeier
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Patent number: 9732105Abstract: A compound having a structure of:Type: GrantFiled: November 13, 2015Date of Patent: August 15, 2017Assignee: University of Pittsburgh—Of the Commonwealth System of Higher EducationInventors: Peter Wipf, Marie-Celine Frantz
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Patent number: 9701948Abstract: The present disclosure provides recombinant E. coli and other bacteria with reduced alcohol dehydrogenase and/or aldehyde reductase activity. The present disclosure further provides recombinant E. coli and other bacteria with reduced isobutyraldehyde reductase activity. Methods for the production and the uses of the recombinant bacteria are also provided. Specifically, recombinant bacteria further expressing 2-keto-acid decarboxylase can be used for producing higher aldehydes or other non-alcohol chemicals derived from aldehydes.Type: GrantFiled: June 18, 2013Date of Patent: July 11, 2017Assignee: The Regents Of The University Of CaliforniaInventors: Shota Atsumi, Gabriel Rodriguez
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Patent number: 9587261Abstract: The present invention relates to microorganisms of Escherichia coli having enhanced L-tryptophan productivity and to a method for producing L-tryptophan using the same. More particularly, the present invention relates to an Escherichia coli variant in which repression and attenuation control of the tryptophan operon is released and accumulation of anthranilate is reduced and thereby enhancing L-tryptophan productivity. The present invention also relates to a method for producing L-tryptophan using the Escherichia coli variant.Type: GrantFiled: January 10, 2013Date of Patent: March 7, 2017Assignee: CJ Cheiljedang CorporationInventors: Kwang Ho Lee, Hye Min Park, Hyo Hyoung Lee, Young Bin Hwang, Seok Myung Lee
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Patent number: 9518280Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: GrantFiled: October 28, 2014Date of Patent: December 13, 2016Assignee: CARGILL, INCORPORATEDInventors: Ellen Burke, Steven John Gort, Paula M. Hicks, Peter Luginbuhl, Sara C. McFarlan, Toby Richardson, Christopher Solheid, David P. Weiner, Lishan Zhao
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Patent number: 9493777Abstract: The present invention provides a method for producing L-amino acids or salts thereof by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to overexpress the yajL gene.Type: GrantFiled: August 10, 2016Date of Patent: November 15, 2016Assignee: AJINOMOTO CO., INC.Inventors: Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova, Vera Georgievna Doroshenko
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Patent number: 9441253Abstract: The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.Type: GrantFiled: February 7, 2014Date of Patent: September 13, 2016Assignee: William Marsh Rice UniversityInventors: Ka-Yiu San, George N. Bennett, Hui Wu
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Patent number: 9428726Abstract: The present invention provides for a modified host cell comprising a heterologous expression of an efflux pump capable of transporting an organic molecule out of the host cell wherein the organic molecule at a sufficiently high concentration reduces the growth rate of or is lethal to the host cell.Type: GrantFiled: May 25, 2011Date of Patent: August 30, 2016Assignee: The Regents of the University of CaliforniaInventors: Mary J. Dunlop, Jay D. Keasling, Aindrila Mukhopadhyay
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Patent number: 9180081Abstract: Improved formulations for transdermal delivery of botulinum toxin are disclosed. The formulations include, for example, botulinum toxin non-covalently associated with a positively charged backbone having branching or efficiency groups. The formulations also include a partitioning agent, oligo-bridge, or polyanion bridge, and may optionally contain a viscosity modifying agent. The formulations are designed for topical application onto the skin of a patient and may be used to treat wrinkles, hyperhidrosis, and other health-related problems. Kits for administration are also described.Type: GrantFiled: March 3, 2006Date of Patent: November 10, 2015Assignee: REVANCE THERAPEUTICS, INC.Inventors: Michael D. Dake, Jacob M. Waugh
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Patent number: 9081199Abstract: The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.Type: GrantFiled: March 17, 2006Date of Patent: July 14, 2015Assignee: KYOWA HAKKO BIO CO., LTD.Inventors: Kuniki Kino, Yuji Nakazawa, Makoto Yagasaki
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Publication number: 20150147788Abstract: The present invention relates to microorganisms of Escherichia coli having enhanced L-tryptophan productivity and to a method for producing L-tryptophan using the same. More particularly, the present invention relates to an Escherichia coli variant in which repression and attenuation control of the tryptophan operon is released and accumulation of anthranilate is reduced and thereby enhancing L-tryptophan productivity. The present invention also relates to a method for producing L-tryptophan using the Escherichia coli variant.Type: ApplicationFiled: January 10, 2013Publication date: May 28, 2015Inventors: Kwang Ho Lee, Hye Min Park, Hyo Hyoung Lee, Young Bin Hwang, Seok Myung Lee
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Publication number: 20150140614Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.Type: ApplicationFiled: January 28, 2015Publication date: May 21, 2015Applicant: EVONIK DEGUSSA GMBHInventors: ALEXANDER RETH, BRIGITTE BATHE, STEPHAN HANS, WILFRIED CLAES
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Patent number: 9034610Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: GrantFiled: April 4, 2013Date of Patent: May 19, 2015Assignee: CARGILL, INCORPORATEDInventors: Timothy W. Abraham, Douglas C. Cameron, Paula M. Hicks, Sara C. McFarlan, James R. Millis, John Rosazza, David P. Weiner, Lishan Zhao
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Patent number: 9005938Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: GrantFiled: September 16, 2011Date of Patent: April 14, 2015Assignee: Cargill, IncorporatedInventors: Ellen Burke, Steven John Gort, Paula M. Hicks, Peter Luginbuhl, Sara C. McFarlan, Toby Richardson, Christopher Solheid, David Weiner, Lishan Zhao
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Patent number: 8975046Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.Type: GrantFiled: November 2, 2011Date of Patent: March 10, 2015Assignee: Cargill, IncorporatedInventors: Brian J. Brazeau, Mervyn L. De Souza, Steven J. Gort, Paula M. Hicks, Sherry R. Kollmann, Jose M. Laplaza, Sara C. McFarlan, Fernando A. Sanchez-Riera, Christopher Solheid
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Patent number: 8975045Abstract: A method for efficiently producing an L-amino acid utilizing a bacterium belonging to the family Enterobacteriaceae from a fatty acid or an alcohol such as glycerol as a raw material is provided. A bacterium belonging to the family Enterobacteriaceae which is able to produce L-amino acid and harbors an RpsA protein which has a mutation such that the native aspartic acid residue at position 210 is replaced with another amino acid residue is used. This bacterium is cultured in a medium containing a carbon source selected from a fatty acid and an alcohol, and the produced L-amino acid is collected from the medium.Type: GrantFiled: August 8, 2012Date of Patent: March 10, 2015Assignee: Ajinomoto Co., Inc.Inventors: Hidetaka Doi, Yusuke Hagiwara, Yoshihiro Usuda
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Publication number: 20150056666Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.Type: ApplicationFiled: November 4, 2014Publication date: February 26, 2015Applicant: Evonik Degussa GmbHInventors: Alexander RETH, Brigitte Bathe, Stephan Hans, Wilfried Claes
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Publication number: 20150056667Abstract: The present invention relates to a hydantoinase having an amino acid sequence selected from (i) or (ii), with (i) amino acid sequence selected from SEQ ID NO: 6-20 and SEQ ID NO: 73-119 (ii) amino acid sequence wherein in the amino acid sequence of SEQ ID NO: 6-20 and SEQ ID NO: 73-119, 1 to 75 amino acid residues have been substituted, deleted, inserted and/or added, and wherein further the catalytic activity of the hydantoinase is higher by a factor of at least 1.2 than the catalytic activity of the hydantoinase having amino acid sequence SEQ ID NO: 1, The present invention further relates to a process for preparing amino acids, wherein said hydantoinase is used.Type: ApplicationFiled: November 16, 2012Publication date: February 26, 2015Inventors: Steffen Osswald, Heiko Schuster, Jürgen Roos, Andreas Karau, Ulrich Schwaneberg, Ronny Martinez, Hemanshu Mundhada, Ursula Holter
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Publication number: 20150056665Abstract: A method of producing a chemical includes culturing cells in a culture solution in a fermentor to ferment a feedstock to produce a chemical; supplying the culture solution containing the chemical produced in the culturing to a plurality of separation membrane units arranged in parallel; filtering the culture solution supplied in the supplying to separate a permeate containing the chemical; refluxing a retentate that is not filtered in the filtering to the fermentor; and supplying a gas containing oxygen to the plurality of separation membrane units while a supply amount is changed to at least two different values to perform scrubbing, wherein the supply amount and supply time of the gas containing oxygen supplied in the culturing and the supplying the gas are set so that a kLa value is within a predetermined range from an optimal kLa value for the cells cultured in the culturing.Type: ApplicationFiled: March 27, 2013Publication date: February 26, 2015Inventors: Satoko Kanamori, Hideki Sawai, Norihiro Takeuchi
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Publication number: 20150050703Abstract: The present invention relates to a microorganism able to produce L-threonine or L-tryptophan, and to a method for producing L-threonine or L-tryptophan by using same. More specifically, the present invention relates to: recombinant Escherichia coli which is more efficient in producing L-threonine or L-tryptophan by increasing the ability to produce ATP which is used as the most plentiful energy source in cells when producing L-threonine or L-tryptophan; and a method for producing L-threonine or L-tryptophan by using same.Type: ApplicationFiled: January 7, 2013Publication date: February 19, 2015Inventors: Ki Yong Cheong, Seok Myung Lee, Young Bin Hwang, Keun Cheol Lee, Kwang Ho Lee
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Publication number: 20150044734Abstract: The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom.Type: ApplicationFiled: August 31, 2012Publication date: February 12, 2015Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFONIAInventors: Darmawi Juminaga, Jay D. Keasling
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Publication number: 20150037849Abstract: Recombinant microbial cells and methods for producing 5-hydroxytryptophan (5HTP) using such cells are described. More specifically, the recombinant microbial cell comprises an exogenous gene encoding an L-tryptophan hydroxylase, and means for providing tetrahydrobiopterin (THB). Related sequences and vectors for use in preparing such recombinant microbial cells are also described.Type: ApplicationFiled: February 28, 2013Publication date: February 5, 2015Inventors: Eric Michael Knight, Jiangfeng Zhu, Jochen Förster, Hao Luo
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Patent number: 8945907Abstract: The present invention relates to a microorganism having L-tryptophan productivity and a method for producing L-tryptophan using the same. More precisely, the present invention relates to the recombinant E. coli strain CJ600 (KCCM 10812P) having tryptophan productivity produced from the mutant form (KFCC 10066) of E. coli having L-phenylalanine productivity, wherein tryptophan auxotrophy is released, L-phenylalanine biosynthesis is blocked but tryptophan productivity is enhanced by reinforcing the gene involved in tryptophan biosynthesis, and a method of producing L-tryptophan using the same.Type: GrantFiled: December 28, 2007Date of Patent: February 3, 2015Assignee: CJ Cheiljedang CorporationInventors: Jae-yeong Ju, Hyang Choi, Eun-sung Koh, Ji-sun Lee, Jin-ho Lee, So-young Kim, Chang-hyun Jin, Young-hoon Park
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Publication number: 20140377814Abstract: The present invention relates to a method for biologically treating carbon dioxide using the sulfur-oxidizing chemolithoautotroph Sulfurovum lithotrophicum 42BKT. The method of the present invention may enable carbon dioxide to be fixed or converted in high-concentration and high-pressure conditions which do not allow the biological photosynthetic conversion of microalgae or the like, and may exhibit high efficiency in the fixation of carbon dioxide as compared to existing methods for biologically treating carbon dioxide using microalgae. Further, the method of the present invention may use a gas mixture without a process of separating nitrogen and other gases, thus simplifying the process of the fixation or conversion of carbon diode.Type: ApplicationFiled: September 20, 2012Publication date: December 25, 2014Inventors: Chang Ha Lee, Ik Sung Ahn, Hyuk Sung Kwon, Jae Hyuk Lee
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Patent number: 8895270Abstract: Disclosed is a method for producing a ?-amino acid comprising a step of synthesizing a ?-amino acid from an ?-amino acid in the presence of an amino acid aminomutase. In this method, a ?-amino acid is precipitated as a solid in the reaction solution.Type: GrantFiled: September 30, 2008Date of Patent: November 25, 2014Assignee: Mitsui Chemicals, Inc.Inventor: Tomonori Hidesaki
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Publication number: 20140315261Abstract: The present invention provides a method for producing L-amino acids by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the yjjK gene.Type: ApplicationFiled: April 23, 2014Publication date: October 23, 2014Applicant: AJINOMOTO CO., INC.Inventors: Natalia Viktorovna Stoynova, Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Evgeniya Aleksandrovna Polyakova
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Patent number: 8859243Abstract: The present invention provides a method for producing an L-amino acid by fermentation by culturing a microorganism having an L-amino acid-producing ability in a liquid medium to precipitate the L-amino acid, wherein a polymer such as a water-soluble cellulose derivative, a water-soluble polyvinyl compound, a polar organic solvent-soluble polyvinyl compound, a water-soluble starch derivative, an alginic acid salt, and a polyacrylic acid salt is added to the medium.Type: GrantFiled: April 7, 2009Date of Patent: October 14, 2014Assignee: Ajinomoto Co., Inc.Inventors: Satoshi Okutani, Shinya Fujiki, Shintaro Iwatani
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Publication number: 20140287472Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.Type: ApplicationFiled: June 11, 2014Publication date: September 25, 2014Applicant: AJINOMOTO CO., INC.Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
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Patent number: 8835154Abstract: A microorganism of the genus Escherichia having enhanced L-amino acid productivity, wherein the microorganism is transformed to have an enhanced NAD kinase activity and an inactivated activity of an enzyme having an amino acid sequence of SEQ ID NO: 2 encoded by tehB gene and a method for producing L-amino acids using the microorganism of the genus Escherichia.Type: GrantFiled: January 18, 2012Date of Patent: September 16, 2014Assignee: CJ Cheiljedang CorporationInventors: Kwang Ho Lee, Keun Chul Lee, Seok Myung Lee, Young Bin Hwang
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Publication number: 20140227745Abstract: The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.Type: ApplicationFiled: February 7, 2014Publication date: August 14, 2014Applicant: William Marsh Rice UniversityInventors: Ka-Yiu SAN, George N. BENNETT, Hui WU
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Publication number: 20140212931Abstract: The present invention concerns iodoperoxidases from Zobellia galactanivorans, isolated nucleic acids encoding same, as well as methods for preparing these enzymes. Moreover, the invention is also directed to the use of such iodoperoxidases in a wide range of industrial, pharmaceutical, medical, cosmetics, and ecological applications, as well as in the food industry.Type: ApplicationFiled: July 5, 2012Publication date: July 31, 2014Applicants: Universite Pierre et Marie Curie (Paris 6), Centre National de la Recherche Scientifique (CNRS)Inventors: Catherine Leblanc, Ludovic Delage, Gurvan Michel, Etienne Rebuffet, Mirjam Czjzek, Philippe Potin
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Patent number: 8785162Abstract: The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.Type: GrantFiled: December 31, 2008Date of Patent: July 22, 2014Assignee: Cargill, IncorporatedInventors: Mervyn L. De Souza, Paula M. Hicks, Sherry R. Kollmann, Jose Laplaza, Joshua M. Lundorff, Sara C. McFarlan, Erin Marasco, Wei Niu, Fernando A. Sanchez-Riera, Christopher Solheid, David P. Weiner, Peter Luginbuhl, Analia Bueno, Joslin Cuenca
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Patent number: 8771997Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.Type: GrantFiled: October 12, 2011Date of Patent: July 8, 2014Assignee: Ajinomoto Co., Inc.Inventors: Yasuaki Takakura, Hiroomi Ogino, Masakazu Sugiyama, Kenichi Mori, Eri Tabuchi, Koki Ishikawa, Uno Tagami, Hidemi Fujii
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Publication number: 20140162325Abstract: The present invention provides a method for producing L-amino acid using a bacterium belonging to the family Enterobacteriaceae, particularly a motile bacterium belonging to the genus Escherichia, Enterobacter or Pantoea, wherein the bacterium has been modified so that expression of at least one gene of the flagella formation and motility cascade is enhanced.Type: ApplicationFiled: February 11, 2014Publication date: June 12, 2014Applicant: AJINOMOTO CO., INC.Inventors: Irina Borisovna Altman, Tatyana Abramovna Yampolskaya, Leonid Romanovich Ptitsyn