Sugar Acid Having Five Or More Carbon Atoms (i.e., Aldonic, Keto-aldonic, Or Saccharic Acid) Patents (Class 435/137)
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Publication number: 20130269061Abstract: Genetically engineered plants having altered levels of one or more starch regulation enzymes and a polysaccharide degrading enzyme are provided. Methods of genetically engineering plants to express products altering expression of one or more starch regulation enzymes and polysaccharide degrading enzymes, and genetic constructs are provided. Methods of agricultural processing and animal feed using the genetically engineered plants are described.Type: ApplicationFiled: March 11, 2013Publication date: October 10, 2013Inventors: Philip A. Lessard, Michael Lanahan, Vladimir Samoylov, Oleg Bougri, Jonas Emery, R. Michael Raab, Dongcheng Zhang
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Publication number: 20130260423Abstract: The present invention relates to methods of saccharifying the trash (leaf) fraction of sugar cane using enzymes.Type: ApplicationFiled: October 26, 2011Publication date: October 3, 2013Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Benjamin Knudsen, Armindo Ribeiro Gaspar
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Publication number: 20130260420Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 26, 2012Publication date: October 3, 2013Inventors: Marc D. Morant, Paul Harris
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Publication number: 20130210087Abstract: The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 4, 2011Publication date: August 15, 2013Applicant: NOVOZYMES INC.Inventors: Ye Liu, Lan Tang
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Publication number: 20130203126Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: April 5, 2013Publication date: August 8, 2013Applicant: NOVOZYMES A/SInventor: NOVOZYMES A/S
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Publication number: 20130196396Abstract: The present disclosure relates to hydrolysis of hexose-?-lactones by use of an S. thermophile extracellular aldonolactonase. In particular the present disclosure relates to compositions including a S. thermophile extracellular aldonolactonase and methods of use thereof.Type: ApplicationFiled: June 23, 2011Publication date: August 1, 2013Applicant: The Regents of the University of CaliforniaInventors: William T. Beeson, IV, James H. Doudna Cate, Michael A. Marletta
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Publication number: 20130189747Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a sulfur-containing compound. The present invention also relates to methods of using the compositions.Type: ApplicationFiled: August 5, 2011Publication date: July 25, 2013Applicant: NOVOZYMES, INC.Inventor: Feng Xu
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Publication number: 20130183713Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 30, 2011Publication date: July 18, 2013Applicants: NOVOYZMES A/S, NOVOZYMES, INC.Inventor: Marc Morant
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Publication number: 20130157314Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a heterocyclic compound. The present invention also relates to methods of using the compositions.Type: ApplicationFiled: August 5, 2011Publication date: June 20, 2013Applicant: NOVOZYMES, INC.Inventors: Feng Xu, Matthew Sweeney, Jason Quinlan
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Publication number: 20130157307Abstract: The invention relates to a process of fermenting plant material in a fermentation medium into a fermentation product using a fermenting organism, wherein one or more deamidases are present in the fermentation medium.Type: ApplicationFiled: August 2, 2011Publication date: June 20, 2013Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Chee-Leong Soong, Shiro Fukuyama, Allan Noergaard, Preben Nielsen, Peter Rahbek Oestergaard
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Patent number: 8466135Abstract: A hair treatment composition comprising at least one lactone in combination with at least one disaccharide.Type: GrantFiled: September 8, 2008Date of Patent: June 18, 2013Assignee: Conopco, Inc.Inventors: Masahiro Kasai, Kayo Kobori
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Publication number: 20130143278Abstract: The present invention provides a process for producing fermentable sugar or a fermentation product from a lignocellulosic feedstock. The process comprises leaching the lignocellulosic feedstock with an aqueous solution to remove at least potassium salts from the lignocellulosic feedstock and without significantly hydrolyzing hemicellulose and cellulose, thereby producing a leached feedstock and leachate. The leachate is removed from the leachate and concentrated. The leached feedstock is hydrolyzed to produce fermentable sugar, which may be fermented to produce a fermentation broth comprising the fermentation product. The concentrated leachate is recirculated to one or more stages in the process involving alkali addition to adjust the pH of a process stream.Type: ApplicationFiled: August 11, 2011Publication date: June 6, 2013Applicant: Iogen Energy CorporationInventors: Jeffrey S. Tolan, Daphne Wahnon, David Geros
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Publication number: 20130130331Abstract: A method is provided for producing sugars using a combination of acids to hydrolyze hemicellulosic and cellulosic materials in biomass, said combination of acids namely comprising a first, weak organic acid (such as acetic acid or formic acid) for providing a pentose product or stream from hydrolyzing hemicellulosic materials in the biomass on a batchwise, semi-continuous or continuous basis, and a second, strong mineral acid (such as sulfuric acid) for providing a hexose product or stream from hydrolyzing cellulosic materials in the biomass.Type: ApplicationFiled: January 18, 2011Publication date: May 23, 2013Applicant: ARCHER DANIELS MIDLAND COMPANYInventors: Thomas P. Binder, Paul D. Bloom, Perry H. Doane, Chi-Cheng Ma
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Publication number: 20130084612Abstract: Disclosed herein is a recombinant Escherichia coli (E. coli) capable of producing D-xylonic acid from D-xylose and a method for producing D-xylonic acid using the same. The recombinant E. coli producing D-xylonic acid from D-xylose according to the present invention is a recombinant E. coli EWX4 (Microorganism deposition number KCTC11988BP) capable of producing D-xylonic acid from D-xylose. When utilizing the recombinant E. coli prepared by the method of the present invention, it is possible to produce D-xylonic acid from D-xylose with high yield while reducing production cost using sole carbon source.Type: ApplicationFiled: August 21, 2012Publication date: April 4, 2013Inventors: Wook-Jin Chung, Huaiwei Liu, Kris Nino Gomez Valdehuesa, Kristine Rose Medina Ramos, Mi-Deok Han
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Publication number: 20130071894Abstract: Aspects of the invention relate to the design and construction of Metabolite Valves, such as Glucose Valves, that can be used to divert metabolites from endogenous pathways toward alternative pathways in a cell.Type: ApplicationFiled: March 30, 2011Publication date: March 21, 2013Applicant: Massachusetts Institute of TechnologyInventors: Kevin Solomon, Tae Seok Moon, Kristala Lanett Jones Prather
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Publication number: 20130052693Abstract: The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.Type: ApplicationFiled: July 13, 2012Publication date: February 28, 2013Applicant: CODEXIS, INC.Inventors: DIPNATH BAIDYAROY, LOUIS CLARK, DAVID ELGART, RAMA VOLADRI, XIYUN ZHANG
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Patent number: 8383375Abstract: The present invention relates to a novel thermostable gluconate dehydratase from the thermoacidophilic archaeon Sulfolobus solfataricus, a coding sequence, and an expression system. The gluconate dehydratase has a molecular weight of about 320,000 to 380,000 daltons as the native protein, and about 40,000 to 50,000 daltons as the monomer protein, and catalyzes the dehydration reaction of aldonic acids to 2-keto-3-deoxy derivatives at temperatures of less than 120° C. The gluconate dehydratase can be produced from native or recombinant host cells and thereby used in the pharmaceutical, agricultural, and other industries.Type: GrantFiled: October 22, 2010Date of Patent: February 26, 2013Assignees: Posco, Postech FoundationInventors: Seonghun Kim, Sun Bok Lee
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Publication number: 20130017571Abstract: A truncated pullulanase variant of a parent pullulanase belonging to family GH57 comprising an X47 domain and the use thereof.Type: ApplicationFiled: December 22, 2010Publication date: January 17, 2013Applicant: NOVOZYMES A/SInventors: Martin Borchert, Morten Gjermansen, Suzanne Clark, Bernard Henrissat, Maria B. Silow, Peter F. Hallin
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Publication number: 20120276594Abstract: The present invention relates to cellobiohydrolase variants having improved thermostability and/or thermoactivity in comparison to wild-type Myceliophthora thermophila CBH2b.Type: ApplicationFiled: April 27, 2012Publication date: November 1, 2012Applicant: Codexis, Inc.Inventors: Rama Voladri, Xiyun Zhang, Sachin Patil, David Elgart, Gregory Miller, Louis Clark, Kui Chan
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Publication number: 20120276585Abstract: The present invention relates to a method for producing a hydrolysate of from lignocellulose-containing material, comprising pre-treatment with low temperature, hydrolysis and fermentation, wherein hydrolysis is performed by contacting the lignocellulose-containing material with an enzyme composition comprising at least 10% xylanase enzyme protein w/w%.Type: ApplicationFiled: December 21, 2010Publication date: November 1, 2012Applicants: COFCO CORPORATION, NOVOZYMES A/SInventors: Haiyu Ren, Dongmin Li, Yun Wang, Chuanji Fang, Hong Zhi Huang
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Publication number: 20120237979Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 30, 2010Publication date: September 20, 2012Applicant: NOVOZYMES A/SInventors: Junxin Duan, Kirk Matthew Schnorr, Wenping Wu
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Publication number: 20120214196Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 30, 2010Publication date: August 23, 2012Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
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Publication number: 20120214209Abstract: The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction of the substrate.Type: ApplicationFiled: April 27, 2012Publication date: August 23, 2012Applicant: Danisco US Inc.Inventors: Gopal K. Chotani, Manoj Kumar, Jeff P. Pucci, Karl J. Sanford, Jayarama K. Shetty
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Patent number: 8241878Abstract: Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.Type: GrantFiled: November 15, 2011Date of Patent: August 14, 2012Assignee: Butamax(TM) Advanced Biofuels LLCInventors: Larry Cameron Anthony, Dennis Flint, Wonchul Suh, Rick W. Ye, Steven Cary Rothman, Jean-Francois Tomb
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Patent number: 8232079Abstract: After various sugar nucleotide solutions and glycosyltransferases (or primers) have been mixed, they are introduced into a reaction tank (column) with primers (or glycosyltransferases) immobilized thereon. Then solutions coming out of the reaction tank are led to an ultrafiltration column. The oligosaccharide synthesizer according to the present invention is equipped with a flow path for ensuring that glycosyltransferases or primers separated by the ultrafiltration column are returned into a container for storing each solution in a sample injector.Type: GrantFiled: August 5, 2005Date of Patent: July 31, 2012Assignee: Hitachi High-Technologies CorporationInventors: Kisaburo Deguchi, Genzou Hirata, Masahito Ito, Hiroaki Nakagawa, Shinichiro Nishimura
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Patent number: 8178326Abstract: The invention relates to the utilization of fatty materials with substantial free fatty acid content in the production of biodiesel by the use of microbial enzymes that are effective in a solvent-free process for the production of esters of fatty acids and C1-C3 alkyl alcohols.Type: GrantFiled: April 9, 2008Date of Patent: May 15, 2012Assignees: Novozymes A/S, N.V. Desmet Ballestra Group S.A.Inventors: Wim De Greyt, Marc Kellens, Hans Christian Holm, Morten Wurtz Christensen, Per Munk Nielsen
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Patent number: 8173404Abstract: A process for obtaining fermentable sugars from barley, involving treating hulled barley with H2SO4 to produce acid treated barley and treating the acid treated barley with enzymes to produce fermentable sugars.Type: GrantFiled: March 17, 2009Date of Patent: May 8, 2012Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: Tae Hyun Kim, Kevin Hicks, Frank Taylor
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Patent number: 8142620Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: GrantFiled: August 3, 2010Date of Patent: March 27, 2012Assignee: Xyleco, Inc.Inventor: Marshall Medoff
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Patent number: 8137941Abstract: A chondroitin polymerase having such properties that it transfers GlcUA and GalNAc alternately to a non-reduced terminal of a sugar chain from a GlcUA donor and a GalNAc donor, respectively, and the like; and a process for producing the chondroitin polymerase.Type: GrantFiled: August 10, 2009Date of Patent: March 20, 2012Assignee: Seikagaku CorporationInventors: Toshio Ninomiya, Nobuo Sugiura, Koji Kimata
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Publication number: 20120064585Abstract: Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.Type: ApplicationFiled: November 15, 2011Publication date: March 15, 2012Applicant: BUTAMAXTM ADVANCED BIOFUELS LLCInventors: Larry Cameron Anthony, Lori Ann Maggio-Hall, Steven Cary Rothman, Jean-Francois Tomb
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Publication number: 20120064586Abstract: The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.Type: ApplicationFiled: September 9, 2011Publication date: March 15, 2012Inventors: Robert M. Caldwell, M. Harunur Rashid, Fernando Valle
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Patent number: 8097448Abstract: Cells of the species Issatchenkia orientalis and closely related yeast species are transformed with a vector to introduce an exogenous lactate dehydrogenase gene. The cells produce lactic acid efficiently and are resistant at low pH, high lactate titer conditions.Type: GrantFiled: May 30, 2006Date of Patent: January 17, 2012Assignee: Cargill Inc.Inventors: Pirkko Suominen, Aristos Aristidou, Merja Pentilla, Marja Ilmen, Laura Ruohonen, Kari Koivuranta, Kevin Roberg-Perez
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Publication number: 20120009635Abstract: The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.Type: ApplicationFiled: September 9, 2011Publication date: January 12, 2012Applicant: Danisco US Inc.Inventors: Timothy C. Dodge, Manoj Kumar, M. Harunur Rashid, Fernando Valle
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Publication number: 20120005788Abstract: Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolysed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolysed lignocellulosic plant biomass.Type: ApplicationFiled: March 17, 2010Publication date: January 5, 2012Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTTInventors: Peter Richard, Marilyn Wiebe, Mervi Toivari, Dominik Mojzita, Laura Ruohonen, Merja Penttilä
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Publication number: 20110229942Abstract: Microorganisms for the production of high-value chemicals from free fatty acids are provided. The microorganisms comprise genetic mutations that alter fatty acid metabolism. The genetic mutations include a mutation or deletion of a fadR gene in which the FadR enzyme activity is partially or substantially eliminated and a mutation in an atoC gene that provides overexpression of the microorganism's ato operon. Methods of using the microorganisms to produce high-value chemicals are also provided. The high-value chemicals include ethanol, methyl acetate, succinate, gamma-butyrolactone, 1,4-butanediol, acetone, iso-propanol, butyrate, butanol, mevalonate, propionate, ethanolamine and 1,2-propanediol.Type: ApplicationFiled: December 15, 2008Publication date: September 22, 2011Applicant: Glycos Biotechnologies, IncorporatedInventors: Paul Campbell, Ramon Gonzalez
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Patent number: 8017981Abstract: Wide bandgap semiconductor devices including normally-off VJFET integrated power switches are described. The power switches can be implemented monolithically or hybridly, and may be integrated with a control circuit built in a single- or multi-chip wide bandgap power semiconductor module. The devices can be used in high-power, temperature-tolerant and radiation-resistant electronics components. Methods of making the devices are also described.Type: GrantFiled: June 29, 2010Date of Patent: September 13, 2011Assignee: SemiSouth Laboratories, Inc.Inventors: Igor Sankin, Joseph Neil Merrett
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Publication number: 20110201061Abstract: Disclosed are a method of hydrolysis of wet fiber and a method for preparing ethanol. Generally, an agricultural plant material, such as corn hulls, distiller's dried grains, or spent germ, is treated to at least partially hydrolyze the fiber. The process may include a maceration step followed by a shearing operation in the presence of steam to yield a treated product, in which, in many embodiments, saccharides will be released and unbound from fibrous portions of the agricultural product. In some embodiments, the process includes macerating the material to provide a slurry having a solids content of at least 10 percent and jet cooking the slurry. A mixture of saccharides prepared in this fashion may be fermented to yield ethanol and/or biochemicals.Type: ApplicationFiled: February 16, 2011Publication date: August 18, 2011Inventors: Sarjit Johal, Steven L. Ramsden, Zachary J. Halloran, Albert J. Pollmeier
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Publication number: 20110183382Abstract: This invention provides systems and methods for the production of compounds by C. phytofermentans. C. phytofermentans is genetically-engineered for hydrolysis and fermentation of carbonaceous biomass to synthesize compounds of commercial value.Type: ApplicationFiled: December 15, 2010Publication date: July 28, 2011Applicant: Qteros, Inc.Inventors: Matthias Schmalisch, Chelsea Ju, Francis H. Verhoff, Gregory S. Coil
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Publication number: 20110177567Abstract: The invention relates to a method for the production of a fermentation product from lignocellulosic biomass, to a reactor to carry out the method and to use of the reactor to produce a fermentation product.Type: ApplicationFiled: August 12, 2008Publication date: July 21, 2011Inventors: Robert Reurd Christophor Bakker, Edserd De Jong, Ronald Hubertus Wilhelmus Maas, Ruud Alexander Weusthuis, Diana Visser, Hendrik Martinus Winkelaar, Mickel Leonardus August Jansen
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Publication number: 20110124065Abstract: The invention relates to the production of glucuronic and glucaric acid in cells through recombinant expression of myo-inositol 1-phosphate synthase, myo-inositol oxygenase and uronate dehydrogenase. Cloning and characterization of the gene encoding uronate dehydrogenase is also disclosed.Type: ApplicationFiled: April 3, 2009Publication date: May 26, 2011Applicant: Massachusetts Institute of TechnologyInventors: Tae Seok Moon, Sang-Hwal Yoon, Kristala Lanett Jones Prather
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Publication number: 20110111471Abstract: The present invention relates to a novel thermostable gluconate dehydratase from the thermoacidophilic archaeon Sulfolobus solfataricus, a coding sequence, and an expression system. The gluconate dehydratase has a molecular weight of about 320,000 to 380,000 daltons as the native protein, and about 40,000 to 50,000 daltons as the monomer protein, and catalyzes the dehydration reaction of aldonic acids to 2-keto-3-deoxy derivatives at temperatures of less than 120° C. The gluconate dehydratase can be produced from native or recombinant host cells and thereby used in the pharmaceutical, agricultural, and other industries.Type: ApplicationFiled: October 22, 2010Publication date: May 12, 2011Inventors: Seonghun Kim, Sun Bok Lee
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Publication number: 20110076730Abstract: Improved enzyme systems, recombinant cells, and processes employing the same to produce biosynthetic D-1,2,4-butanetriol; D-1,2,4-butanetriol prepared thereby and derivatives thereof; D-1,2,4-butanetriol trinitrate prepared therefrom; and enzymes and genes useful in the enzyme systems and recombinant cells.Type: ApplicationFiled: July 19, 2007Publication date: March 31, 2011Applicant: Board of Trustees of Michigan State UniversityInventors: John W. Frost, Wei Niu
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Patent number: 7883873Abstract: The present invention provides a sugar chain synthesizer capable of continuously reacting sugar chains when a plurality of sugar chains are successively reacted. The sugar chain synthesizer of the present invention includes a plurality of vessels containing respective sugar nucleotide solutions, a plurality of vessels containing respective glycosyltransferases, and a reactor containing a primer that is a water-soluble polymer, into which the above described sugar nucleotide solution and glycosyltransferase are introduced. In the present invention, components in a reaction solution obtained in the reactor are separated through an ultrafiltration column, and a reaction product is then returned to the above described reactor, so as to continuously synthesize sugar chains. Although it is a complicated synthesis of sugar chains, it becomes possible to carry out such synthesis continuously and automatically.Type: GrantFiled: February 18, 2005Date of Patent: February 8, 2011Assignee: Hitachi High-Technologies CorporationInventors: Masanori Takaki, Kuriko Yamada, Kisaburo Deguchi, Hiroaki Nakagawa, Shinichiro Nishimura
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Publication number: 20110003353Abstract: The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.Type: ApplicationFiled: August 16, 2010Publication date: January 6, 2011Inventors: Robert M. CALDWELL, M. Harunur Rashid, Fernando Valle
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Publication number: 20100112647Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.Type: ApplicationFiled: October 15, 2009Publication date: May 6, 2010Inventors: Yoshihiko Hara, Keita Fukui, Yoshinori Tajima, Kazue Kawamura, Yoshihiro Usuda, Kazuhiko Matsui
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Patent number: 7579175Abstract: The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.Type: GrantFiled: January 9, 2003Date of Patent: August 25, 2009Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Satoshi Koizumi, Kazuhiko Tabata, Tetsuo Endo, Akio Ozaki
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Publication number: 20090181433Abstract: The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction of the substrate.Type: ApplicationFiled: October 2, 2008Publication date: July 16, 2009Applicant: GENENCOR INTERNATIONAL, INC.Inventors: Gopal K. Chotani, Manoj Kumar, Jeffrey P. Pucci, Karl J. Sanford, Jayarama K. Shetty
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Publication number: 20090104339Abstract: The invention provides for a method for the preparation of a gluconic acid from glucose. It is an aspect of the invention to provide a method that comprises the use of enzymes. The method according to the invention can be applied with great benefit to the production of calcium gluconate suitable for use as a food fortifier by a process that is economical.Type: ApplicationFiled: December 19, 2008Publication date: April 23, 2009Applicant: PURAC BIOCHEM B.V.Inventors: Marcus Johannes Anthonius Wilhelmus Vorage, Diderik Reinder KREMER, Boelem SLOOTS, Johannes Bernardus Maria MEIBERG
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Patent number: 7517675Abstract: A method of oxidising carbohydrates and/or carbohydrate derivatives having primary alcohol groups comprising contacting a reaction medium containing said carbohydrates and/or carbohydrate derivatives with a nitroxy radical mediator and a peroxidase enzyme, characterized in that the initial reaction medium contains at least 10% by weight carbohydrates and/or carbohydrate derivatives, in that the peroxidase enzyme is an oilseed peroxidase and in that a hydroperoxide and an alkali compound are gradually added to the reaction medium such that its pH is maintained at between 3.5 and 10.0 (pH-Stat); use of said method in a process for producing gluconic acid, glucaric acid and/or D-glucuronolactone.Type: GrantFiled: December 17, 2004Date of Patent: April 14, 2009Assignee: Cerestar Holding B.V.Inventors: Ronny Leontina Marcel Vercauteren, An Amanda Jules Heylen
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Patent number: 7510861Abstract: A novel gluconate dehydratase derived from Achromobacter xylosoxidans and a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell containing the gene with an aldonic acid, the corresponding 2-keto-3-deoxyaldonic acid can be efficiently produced.Type: GrantFiled: September 20, 2006Date of Patent: March 31, 2009Assignee: Mitsui Chemicals, Inc.Inventors: Hitoki Miyake, Toshifumi Yamaki, Toshihiro Oikawa, Takeshi Nakamura, Hiroki Ishibashi, Yasushi Fukuiri, Atsushi Sakuma, Hironori Komatsu, Tomoyuki Ando, Kazuhiko Togashi, Hideki Umetani