Abstract: Provided herein are isolation processes and the associated hardware to allow fluid streams to be isolated from a sterilized system (e.g., a sterile process vessel) that contains a sterile process. The isolation processes described herein allow for continuous removal of fluid streams (e.g., waste streams, liquid containing recombinant therapeutic proteins) from a sterilized system (e.g., a biological manufacturing system), which provides for less manual manipulation of the sterilized system and a decreased risk of contaminating the sterilized system.

Abstract: The present invention concerns a method for carrying out the combined operation of a bioethanol production unit and a biogas unit. The method comprises the following steps: a) mashing corn meal from a dry milling step with at least 0.1 t of dry matter in the form of whole stillage and at least 0.1 m3 of outflow from the biogas unit per tonne of corn meal, b) feeding the mash from a) to a cooking stage with mash temperatures below the gelatinization temperature of the starch inn the corn meal, followed by an ethanol-forming fermentation step and then feeding the fermented mash to a distillation step, c) feeding the whole stillage from b) to the mashing step in a) and to the biogas unit.

Abstract: A method for converting an alcohol to a hydrocarbon, the method comprising contacting said alcohol with a metal-loaded zeolite catalyst at a temperature of at least 100° C. and up to 550° C., wherein said alcohol can be produced by a fermentation process, said metal is a positively-charged metal ion, and said metal-loaded zeolite catalyst is catalytically active for converting said alcohol to said hydrocarbon.

Abstract: The present invention provides for novel metabolic pathways to reduce or modulate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous proteins that function to import glycerol and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous proteins or enzymes is activated, upregulated, or downregulated.

Abstract: Ear corn is picked from corn fields by ear corn harvesters and transported to a central shelling station associated with an ethanol manufacturing facility. Shelled corn from the central shelling station is processed into ethanol at the ethanol manufacturing facility, and corn cobs from the central shelling station are burned to provide process heat for the ethanol manufacturing process. Energy is conserved and costs are reduced during the picking and shelling of the ear corn and by the burning of cobs for process heat.

Abstract: The invention provides a process for producing a fermentable gas stream from a gas source that contains one or more constituent which may be harmful to the fermentation process. To produce the fermentable gas stream, the gas stream is passed through a specifically ordered series of removal modules. The removal modules remove and/or convert various constituents found in the gas stream which may have harmful effects on downstream removal modules and/or inhibitory effects on downstream gas fermenting microorganisms. At least a portion of the fermentable gas stream is preferably capable of being passed to a bioreactor, which contains gas fermenting microorganisms, without inhibiting the fermentation process.

Abstract: The present invention relates to novel nucleic acid sequences encoding bacterial xylose isomerases that upon transformation of a eukaryotic microbial host cell, such as yeast, to confer to the host cell the ability of isomerising xylose to xylulose. The nucleic acid sequences encode xylose isomerases that originate from bacteria such as Eubacterium sp., Clostridium cellulosi and others. The invention further relates to fermentation processes wherein the transformed host cells ferment a xylose-containing medium to produce ethanol or other fermentation products.

Abstract: The present disclosure relates to the modulation in the RAS/cAMP/PKA signaling pathway for maintaining the propagation efficiency and increasing fermentation efficiency of yeast cells. The present disclosure provides yeast cells having or engineered to exhibit a modulation in signaling in a RAS/cAMP/PKA pathway, depending on conditions. For example the yeast cells can be selected or genetically modified to express a mutated Ras1 protein, a mutated Ras2 protein, a mutated Ira1 protein and/or a mutated Ira2 protein, optionally in combination with specific promoters. Also provided herewith are methods for propagating the yeast cells as well as using the yeast cells to generate a fermented product (such as ethanol).

Abstract: Methods and systems for the production of one or more products from a gas stream produced in a methanol production process. The method comprises converting at least a portion of a methane feedstock to a substrate comprising CO and H2. The substrate comprising CO and H2 is anaerobically fermented in a bioreactor to produce one or more alcohols. The method and system may further include process for the production of methanol processes for the production of acetic acid.

Abstract: Disclosed are a novel strain of Bacillus amyloliquefaciens, a method of producing fermented grains using the strain, fermented grains produced using the strain, and a composition for thrombolysis; digestion improvement; prophylaxis, amelioration or treatment of bowel inflammation, serous membrane weakening or intestinal injury; or antioxidation, comprising the fermented grains.

Abstract: The present invention provides processes for producing food products, particularly juice beverages, with reduced sugar content enriched with dietary fibers and the food products produced. The processed food products are low in calories while preserving the palatable test of the starting material, and contain beneficial amount of dietary fibers. The processed food product further contains sorbitol and gluconic acid.

Abstract: A fruit wine aroma enhancement brewing technology using cutinase belongs to the technical field of fruit wine brewing. According to the present disclosure, in order to solve the problem that fruit wine prepared through an existing fruit wine fermentation technology is poor in quality such as aroma and taste, Muscat grapes or apples are adopted as raw materials, and after crushing, separating peel residue, performing peel residue heat treatment and enzyme treatment, mixing the treated peel residue and clear juice, adding auxiliary materials (potassium metabisulfite, pectinase and ?-glucosidase), adjusting a sugar degree, inoculating a high-glycerol-field yeast and fermenting with temperature controlled, inoculating an active dry yeast and fermenting with the temperature controlled until fermentation is finished, separating and removing the peel residue, fining, filtering to remove residue, performing stabilizing treatment and performing filtration sterilization, dry grape wine or cider is obtained.

Abstract: The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatement. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.

Abstract: Genetically modified microorganisms useful in fermentation and methods of using such microorganisms are provided. Such microorganisms contain a [GAR+] prion or are modified to contain a [GAR+] prion. Exemplary microorganisms include yeast such as S. cerevisiae. The microorganisms can be further modified to convert xylose and/or arabinose. Methods of fermentation using such microorganisms exhibit improved fermentation efficiency and improved microorganism viability.

Abstract: Methods and compositions for cleaning industrial equipment (e.g. biofuel or corn ethanol plant equipment) in place at high temperatures using acidic methane sulfonic acid compositions having a low pH.

Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment b) optionally washing; c) enzymatic hydrolysis; d) fermentation; and e) optionally recovery of a fermentation product; wherein in step c) an enzyme composition is used that has a temperature optimum of 55 degrees C. or more, the hydrolysis time is 40 hours or more and the temperature is 50 degrees C. or more.

Abstract: The present invention provides for a nucleic acid encoding an isomerase and uses of the isomerase for bioconversion of sugar substrates. The invention represents an advancement in the field of enzyme engineering and discloses a modified nucleic acid for achieving optimum expression of a protein having isomerase activity in a heterologous host. The invention also discloses vectors carrying the modified nucleic acid and recombinant host cells carrying the vectors. The invention also discloses the process for producing a recombinant host cell, process for production of the recombinant enzyme and the process for bioconversion of sugars into their respective isomers using the recombinant protein.

Abstract: The invention describes a process for the production of ethanol from a composition comprising glucose and between 50 ?M and 100 mM acetic acid, said process comprising fermenting said composition in the presence of a recombinant yeast which is capable to convert acetic acid anaerobically; maintaining the amount of undissociated acetic acid at a value of at least 50 ?M; and recovering the ethanol. Said process is useful for both starch and cellulosic based, acetic acid containing hydrolysates and advantageously results in a greater consumption of acetic acid and thus higher ethanol yields.

Abstract: Described herein is a corn protein product comprising greater than about 20% corn protein on a dry weight basis and a free sulfite concentration of less than 150 ppm on an as-is basis. Also disclosed herein is a method to obtain this corn protein product including the steps of providing a protein-rich stream derived from a wet corn milling operation, wherein the protein-rich stream comprises greater than 20% corn protein on a dry weight basis, and treating the protein-rich stream with an oxidant, preferably hydrogen peroxide.

Abstract: The present invention discloses a polypeptide having beta-glucosidase activity. The activity is retained also in high glucose concentration. The invention also discloses an isolated polynucleotide, a nucleic acid construct and a recombinant host usable in production of said polypeptide, and a method for producing the polypeptide. Further the invention discloses compositions including the polypeptide and method of using the polypeptide in hydrolysis or synthesis.

Abstract: A feedstock delivery system transfers a carbonaceous material, such as municipal solid waste, into a product gas generation system. The feedstock delivery system includes a splitter for splitting bulk carbonaceous material into a plurality of carbonaceous material streams. Each stream is processed using a weighing system for gauging the quantity of carbonaceous material, a densification system for forming plugs of carbonaceous material, a de-densification system for breaking up the plugs of carbonaceous material, and a gas and carbonaceous material mixing system for forming a carbonaceous material and gas mixture. A pressure of the mixing gas is reduced prior to mixing with the carbonaceous material, and the carbonaceous material to gas weight ratio is monitored. A transport assembly conveys the carbonaceous material and gas mixture to a first reactor where at least the carbonaceous material within the mixture is subject to thermochemical reactions to form the product gas.

Abstract: The present invention relates to a process for reducing and/or preventing an increase in lactic acid levels in a fermentation product production process, such as especially ethanol production, wherein a lytic polysaccharide monooxygenase (LPMO) or an enzyme composition comprising an LPMO is added before or during saccharification and/or fermentation, or before or during propagation, to reduce and/or prevent an increase in lactic acid levels.

Abstract: A novel isolated Pichia stipitis strain is provided. The strain is capable of fermenting at least a pentose sugar in the presence of one or more inhibitory substances to produce ethanol. A method of utilizing the strain to produce ethanol is also provided.

Abstract: This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.

Abstract: Processes and systems for recovering products from a fermentation mash. In some examples, a process for recovering products from a fermentation mash can include processing a ground corn product to produce a fermentation mash that can include ethanol. At least a portion of the ethanol can be separated from the fermentation mash to produce a whole stillage. The whole stillage can be separated to produce a fiber rich product and a filtrate. The fiber rich product can be hydrolyzed to produce a saccharification mash. The saccharification mash can be processed to produce additional ethanol and a stillage protein product.

Abstract: The invention relates to a recombinant yeast comprising a nucleotide sequence allowing the expression of a glucoamylasey (EC 3.2.1.20 or 3.2.1.3). This cell can be used for the production of ethanol and advantageously produces little or no glycerol.

Abstract: The invention relates to a method and an apparatus for washing a crude lignin slurry (5) formed from plant based raw material, wherein the method comprises separating a soluble carbohydrate containing fraction (10) from the crude lignin slurry (5) by using displacement washing in at least one solid-liquid separation stage (6) so that the crude lignin slurry is prepressed, washed and pressed, and recovering a solid fraction (11) and the soluble carbohydrate containing fraction (10). Further, the invention relates to the soluble carbohydrate containing fraction and the solid fraction, and their uses.

Abstract: Processes and systems for recovering products from a corn fermentation mash. In one example, a process for recovering products from a corn fermentation mash can include separating ethanol from a fermentation mash to produce a whole stillage. The fermentation mash can be derived from a ground corn product milled from a plurality of corn pieces. The plurality of corn pieces can include whole corn kernels, fragmented corn kernels, size-reduced corn kernels, milled corn kernels, or a mixture thereof. Greater than 25 wt % of the ground corn product can have a particle size of greater than 105 ?m and greater than 80 wt % of the ground corn product can have a particle size of 425 ?m or less, as measured according to AOAC 965.22-1966. The process can also include separating the whole stillage to produce a fiber rich portion and a filtrate.

Abstract: A process for producing an organic aliphatic product (such as butanol) from lignocellulosic biomass is provided, comprising: (a) fractionating lignocellulosic biomass in the presence of a solvent for lignin, a hydrolysis catalyst, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin; (b) washing the cellulose-rich solids and separating the cellulose-rich solids from the liquor; (c) enzymatically hydrolyzing the cellulose-rich solids to generate a hydrolysate comprising glucose; (d) detoxifying the hydrolysate by neutralizing the hydrolysate, removing insoluble solids, and removing or oxidizing residual hydrolysis catalyst, thereby generating a purified hydrolysate; (e) fermenting the purified hydrolysate using a suitable microorganism to produce a dilute organic aliphatic product, wherein the microorganism is recycled with a membrane; (f) extracting the dilute organic aliphatic product into a water-immiscible extractant, to generate an intermediate material; and (g) disti

Abstract: Method for producing a fertilizer or herbicide from a whole stillage byproduct produced in a corn dry-milling process for making alcohol and system therefore is disclosed. In one embodiment, the method includes separating the whole stillage byproduct into an insoluble solids portion and a thin stillage portion. Thereafter, the thin stillage portion can be dewatered to provide a water soluble solids portion and a dewatered protein portion, which may be optionally dried. The protein in the resulting protein portion can serve as a nitrogen source and sulfur containing amino acids can serve as a sulfur source, which can be desirable components in fertilizers and herbicides. To that end, the resulting protein portion directly may be sold and/or used as a fertilizer or herbicide or can be combined with other components to provide the fertilizer or herbicide.

Abstract: A method for rehabilitating alcohol, including placing a quantity of ethanol solution in a pressure-controllable environment, decreasing the pressure of the pressure-controllable environment to about 25 Torr, holding the pressure of the pressure-controllable environment at about 25 Torr for a first predetermined period of time, removing unwanted congeners, such as ethyl acetate, from the ethanol solution to yield a treated ethanol solution, and removing treated solution from the pressure-controllable environment.

Abstract: Genetically modified microorganisms that have the ability to convert carbon substrates into chemical products such as 2,3-BDO; 1,4-BDO; isobutyraldehyde; isobutanol; 1-butanol; n-butanol; ethanol; fatty alcohols; and fatty acid methyl ester are disclosed. For example, genetically modified methanotrophs that are capable of generating 2,3-BDO; 1,4-BDO; isobutyraldehyde; isobutanol; 1-butanol; n-butanol; ethanol; fatty alcohols; and fatty acid methyl ester at high titers from a methane source are disclosed. Methods of making these genetically modified microorganisms and methods of using them are also disclosed. These microorganisms and methods make use of molecular switches to regulate gene expression.

Abstract: A process for fermenting syngas is provided which is effective for decreasing an amount of time needed to inoculate a main reactor. The process includes propagating a culture of acetogenic bacteria to provide an inoculum for a main reactor and fermenting syngas in the main reactor.

Abstract: It is disclosed a process to produce a fermentation product from a ligno-cellulosic feedstock hydrolyzate slurry which comprises water, water soluble glucose and xylose and water insoluble pretreated ligno-cellulosic feedstock. The process comprises at least two conversion step. A first conversion medium comprising a first portion of the ligno-cellulosic feedstock hydrolyzate slurry and a yeast capable to ferment glucose and xylose is first created, then the yeast is allowed to convert at least 50% of the glucose and less than 20% of the xylose of the first conversion medium to a first propagated yeast and a first portion of the fermentation product in a first conversion step having a first sugar-to-cells conversion ratio in a range of from 5% to 25%.

Abstract: Apparatuses and associated methods are described for the efficient evaluation of C1-containing substrates, and especially for such evaluation conducted locally, or on-site, at a prospective facility for implementation of a biological conversion process for desired end product using a C1 carbon source. The exact composition of a given, industrial C1-containing substrate, as well as the range in composition fluctuations, are generally difficult to reproduce at a remote facility (e.g., a laboratory or a pilot-scale or demonstration-scale process), as required for the accurate prediction/modeling of commercial performance to justify large capital expenditures for commercial scale-up.

Abstract: The invention relates to production of a yeast strain with increased mannan oligosaccharides, amino acids, and complex carbohydrates. The invention further relates to the process to propagate and ferment such yeast. The invention further relates to methods of use of the yeast as a feed additive for animals and a food product for humans.

Abstract: A process for the production of ethoxylated and hydroxylated lignin fractions, cellulose, lignocellulosic sugars, and ethanol in high yields is provided. The process comprises steaming, pretreatment, chemical recovery, saccharification, and optionally fermentation. A combination of pretreatment conditions is provided resulting in simultaneously high yields of ethoxylated or hydroxylated lignin and cellulose or cellulosic sugars and hernicellulosic sugars. High yield production of ethanol through fermentation can be obtained using this process.

Abstract: In one aspect, the present invention provides a method for preparing a sugar composition. The method includes: forming a mixture including polysaccharide biomass and an ionic liquid solution, wherein the ionic liquid solution contains water and an ionic liquid, and wherein the ionic liquid contains a dicarboxylic acid anion and a cation. The pH of the mixture is greater than or equal to about 10, and the molar ratio of the dicarboxylic acid anion to the cation is at least about 1:2. The method further includes: maintaining the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the polysaccharide biomass; reducing the pH of the mixture containing the dissolved polysaccharide to at least about 7; adding at least one glycoside hydrolase to the mixture having the reduced pH.

Abstract: A method of treating a patient who has melanoma includes administering to said patient a composition containing a population of activated T cells that selectively recognize cells in the patient that aberrantly express a peptide. A pharmaceutical composition contains activated T cells that selectively recognize cells in a patient that aberrantly express a peptide, and a pharmaceutically acceptable carrier, in which the T cells bind to the peptide in a complex with an MHC class I molecule, and the composition is for treating the patient who has melanoma. A method of treating a patient who has melanoma includes administering to said patient a composition comprising a peptide in the form of a pharmaceutically acceptable salt, thereby inducing a T-cell response to the melanoma.

Abstract: Disclosed herein are materials and methods for the production of high concentrations of ethanol from plant starch material broken down into disaccharide and trisaccharide sugars such as maltose, isomaltose, and maltotriose from uncooked starch. Herein is also described use of a yeast strain capable of fermenting high maltose syrups into ethanol without the need to convert disaccharides or trisaccharides into glucose using exogenous glucoamylases.

Abstract: Described herein are recombinant yeast cells expressing a xylulose kinase (XK) which are suitable for fermentation of pentoses. Also described are recombinant yeast cells with higher tolerance to formic and/or acetic acid and suitable for fermentation of pentoses. Also described are recombinant yeast cells expressing an enolase, a phosphofructokinase beta subunit, a 6-phosphofructo-2-kinase, a glucose-6-phosphate isomerase, a phosphoglycerate mutase and/or a triose-phosphate isomerase, and suitable for fermentation of pentoses. Also described are recombinant yeast cells expressing a phosphoglucomutase and/or phosphoribomutase which are suitable for fermentation of pentoses. Further described are methods of using or producing such recombinant yeast cells and related materials.

Abstract: The present disclosure provides processes and systems for dehydrating a byproduct stream in ethanol production. In one embodiment, a feed mixture is distilled with one or more distillation units to remove at least a portion of the water, and form a first byproduct stream. The first byproduct stream is contacted with a molecular sieve unit, thereby forming a product stream. The molecular sieve unit is cyclically contacted with at least a portion of the product stream to regenerate the molecular sieve unit and form one or more regenerate streams. A second byproduct stream including at least one of (1) the regenerate streams and (2) at least a portion of the fusel oil stream is contacted with a separation system, thereby forming a permeate and a retentate. At least a portion of the retentate is forwarded into the product stream.

Abstract: A system and process is disclosed for adding pre-fermentation separated non-fermentables, e.g., fiber, germ/oil, and/or protein, to a post-fermentation stream in a corn (or similar carbohydrate-containing grain) dry milling process for making alcohol and/or other biofuels/biochemical. The process includes mixing grain particles with a liquid to produce a slurry having starch and non-fermentables. The slurry is subjected to liquefaction to convert the starch in the slurry to complex sugars and produce a liquefied stream including the complex sugars and non-fermentables. After liquefaction but prior to fermentation of simple sugars resulting from conversion of the complex sugars, the non-fermentables are separated out to define a non-fermentables portion and an aqueous solution including the complex and/or simple sugars. The simple sugars are fermented to provide a fermented stream. Then, the separated non-fermentables portion is reincorporated back into the process into a post-fermentation stream.

Abstract: A system and method for commercial production of aqueous Sargassum seaweed extract concentrate having arsenic level of around 1.32-15 mg/l; cadmium level of around 0.005-5 mg/l; lead level of around 0.2-12 mg/l; mercury level of around 0.00002-2 mg/l; and pH of around 7.4-9 is disclosed. The resulting seaweed extract concentrate can be used in plant and soil amendments, rooting hormones, other organic plant and soil amendments, organic fertilizers, adjuvants for use with organic pesticides, organic fungicides, in combinations of organic pesticides, with fungicides, and as fertilizers for agricultural, commercial and domestic use. The resulting seaweed extract concentrate can also be used to regulate plant growth, or as a plant nutrient. The method is believed to be able to prepare an extract concentrate from any high protein biomass, including Sargassum, water hyacinth or other seaweed.

Abstract: Disclosed herein is a recombinant microorganism having enhanced 2,3-butanediol producing ability, wherein a pathway for converting pyruvate to acetyl-CoA, a pathway for converting pyruvate to formic acid, or a pathway for converting pyruvate to lactate is inhibited in a microorganism having acetyl-CoA and lactate biosynthetic pathways.

Abstract: A dry-milling ethanol process includes the steps of: dry-milling corn kernels to form a corn flour; combining the corn flour with water to form a mash; fermenting the mash thereby producing beer and carbon dioxide; adding an alkanesulfonic acid to the beer in an amount sufficient to adjust the pH to a range of from about 3 to about 5; distilling the beer to produce ethanol and whole stillage; and processing the whole stillage to produce wet distiller's grains with solubles and/or dried distiller's grains with solubles.

Abstract: The present invention relates to methods for obtaining yeast strains suitable for metabolizing arabinose, and to improved strains with good performance as regards their capacity to ferment arabinose as well as xylose and glucose, including in the presence of inhibitors such as acetic acid.

Abstract: A dry-milling process for the production of dried distiller's grains with solubles (“DDGS”) includes the steps of dry-milling corn kernels to form a corn flour comprising corn fiber; combining the corn flour with water to form a mash; separating the corn fiber from the mash; treating the separated corn fiber with a composition; combining the treated corn fiber with the mash having the corn fiber separated therefrom to form a slurry; fermenting the slurry to produce beer and carbon dioxide; distilling the beer to produce ethanol and whole stillage; and processing the whole stillage to produce DDGS. The composition includes an alkanesulfonic acid, water, an enzyme, and optionally a surfactant.

Abstract: The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.

Abstract: The present disclosure provides recombinant microorganisms capable of sugar co-utilization, the recombinant microorganism comprising a genetically altered microorganism (e.g. S. cerevisiae) having a lack of, or reduced expression of, or expression of truncated or mutated forms of at least one polypeptide selected from Hxk1, Hxk2, and Glk1. Reduction of the expression or biological activity of Hxk1, Hxk2 and Glk1 leads to reduction in the glucose consumption pathway, allowing the microorganism to co-utilize multiple sugars (e.g. glucose, xylose, galactose) at an improved rate.