Carrier-bound Or Immobilized Enzyme Or Microbial Cell; Carrier-bound Or Immobilized Cell; Preparation Thereof Patents (Class 435/174)
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Publication number: 20150073389Abstract: Human hepatocyte cell cultures and their use in bioreactors and bioartificial liver (BAL) systems are provided. The cells have constitutive liver-specific metabolic activity resembling that of freshly isolated human hepatocytes. The hepatocyte cells and BAL systems can be used to treat patients suffering from acute liver failure, end-stage liver disease, or acute-on-chronic liver disease.Type: ApplicationFiled: November 14, 2014Publication date: March 12, 2015Applicant: Academisch Ziekenhuis Bij De Universiteit Van AmsterdamInventors: Robert Antoine Francois Marie CHAMULEAU, Ruurdtje HOEKSTRA, Gerardus Adrianus Antonius NIBOURG
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Publication number: 20150072383Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered polypeptides having transaminase activity, polynucleotides encoding the polypeptides, methods of the making the polypeptides, and methods of using the polypeptides for the biocatalytic conversion of ketone substrates to amine products. The present enzymes have been engineered to have one or more residue differences as compared to the amino acid sequence of the naturally occurring transaminase of Vibrio fluvialis. In particular, the transaminases of the present disclosure have been engineered for efficient formation of chiral tryptamine derivatives from its corresponding prochiral ketone substrates.Type: ApplicationFiled: March 22, 2013Publication date: March 12, 2015Inventors: Jovana Nazor, Derek Smith, Michael Crowe, Shiwei Song, Steven J. Collier
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Publication number: 20150073381Abstract: The invention provides multi-chamber encapsulated cell therapy cartridge devices that are capable of delivering biologically active molecules as well as methods of using these devices.Type: ApplicationFiled: September 10, 2014Publication date: March 12, 2015Inventors: Konrad A. Kauper, John Fraser Mills, Megan Billings, Michael R. Rivera, Alline Monteiro Alcantara Lelis
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Patent number: 8975074Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.Type: GrantFiled: October 15, 2007Date of Patent: March 10, 2015Assignee: The Invention Science Fund I, LLCInventors: Ed Harlow, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
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Patent number: 8975052Abstract: Methods of marking paper products and marked paper products are provided. Some methods include irradiating the paper product to alter the functionalization of the paper.Type: GrantFiled: June 18, 2014Date of Patent: March 10, 2015Assignee: Xyleco, Inc.Inventor: Marshall Medoff
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Patent number: 8969027Abstract: The present invention provides a diagnostic reagent or assay for assessing the activity of a protease in vivo or in vitro and methods of detecting the presence of a cancerous or precancerous cell. The assays are comprised of two particles linked via an oligopeptide linkage that comprises a consensus sequence specific for the target protease. Cleavage of the sequence by the target protease can be detected visually or using various sensors, and the diagnostic results can be correlated with cancer prognosis.Type: GrantFiled: March 3, 2009Date of Patent: March 3, 2015Assignee: Kansas State University Research FoundationInventors: Stefan H. Bossmann, Deryl L. Troyer, Matthew T. Basel
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Publication number: 20150050711Abstract: There is provided a device, and related method and uses, for drawing a polymer fiber, the device comprising: a. at least two polymer compartments, wherein each polymer compartment is capable of retaining a polymer solution, and wherein adjacent compartments comprise different polymer solutions; and b. a slider comprising at least one prong, wherein the prong is capable of contacting the different polymer solutions, and wherein the slider is arranged in a retractable manner from the at least two polymer compartments. There is further provided a system and a related method for manufacturing a polymer fiber.Type: ApplicationFiled: March 18, 2013Publication date: February 19, 2015Inventors: Andrew Chwee Aun Wan, Meng Fatt Leong, Tze Chiun Lim, Jackie Y. Ying, Jerry Kah Chin Toh
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Patent number: 8956823Abstract: An anti-antibody reagent for use in a competitive or sandwich simplex or multiplex assay, said reagent comprising one or more labeled anti-antibodies for the primary antibodies to be determined in the assay, the reagent further comprising a corresponding unlabeled anti-antibody in an excess or near excess concentration with respect to their binding partners.Type: GrantFiled: August 1, 2008Date of Patent: February 17, 2015Assignee: Bio-Rad Laboratories, Inc.Inventors: William F. Link, Renato B. Del Rosario, Randy Sweet, David L. King
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Publication number: 20150044723Abstract: The present invention relates to cofactor regeneration systems, components and uses thereof and methods for generating and regenerating cofactors. The cofactor regeneration system comprises a first electron transfer component selected from a polypeptide comprising a NADH:acceptor oxido-reductase or NADPH:acceptor oxido-reductase, a second electron transfer component selected from a hydrogenase moiety and/or non-biological nanoparticles and an electronically conducting surface. The first and second electron transfer components are immobilised on the electrically conducting surface, and the first and second electron transfer components do not occur together in nature as an enzyme complex.Type: ApplicationFiled: October 3, 2012Publication date: February 12, 2015Inventors: Kylie Vincent, Lars Lauterbach, Oliver Lenz
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Patent number: 8951761Abstract: The disclosed matter relates to immobilized enzymes and methods of use thereof.Type: GrantFiled: June 13, 2006Date of Patent: February 10, 2015Assignee: DSM Nutritional Products AGInventors: Jaroslav A. Kralovec, Weijie Wang
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Patent number: 8951793Abstract: Disclosed are methods of isolating and using a population of FOXP3+ regulatory T cells in a variety of preventative and therapeutic approaches to autoimmune diseases, graft-versus-host disease and transplant rejection.Type: GrantFiled: August 21, 2009Date of Patent: February 10, 2015Assignee: The United States of America, as represented by The Secretary, Department of Health and Human ServicesInventors: Dat Tran, Ethan M. Shevach
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Publication number: 20150037865Abstract: Kits and assemblies for causing, enhancing, and/or expediting consumption of an article by at least one biodegradative living organism are disclosed. The kits and assemblies include at least one additional living organism that enhances and/or expedites consumption of the article by the at least one biodegradative living organism. Methods of producing and using same are also provided.Type: ApplicationFiled: October 21, 2014Publication date: February 5, 2015Inventor: Donald E. Weder
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Patent number: 8945895Abstract: Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange/hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration/diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.Type: GrantFiled: July 30, 2010Date of Patent: February 3, 2015Assignees: Baxter International Inc., Baxter Healthcare S.A.Inventors: Meinhard Hasslacher, Christian Fiedler, Christa Mayer, Artur Mitterer
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Patent number: 8945941Abstract: Aspects of the present invention relate to a method for the preparation of samples for MALDI MS imaging. Certain embodiments relate to a method of matrix deposition for samples, wherein tissue sections are prepared via a synergistic combination of fixation with matrix. In certain embodiments, tissue is fixed with cold solvent, according to well-established histology protocols, and in the presence of matrix, allowing for high resolution spatial mapping of protein, lipid, sugar, and/or nucleic acid distribution. In certain embodiments, the present invention relates to fixation with matrix of whole organisms. In certain embodiments, animals are perfused with fixation and matrix mixtures, which allows for direct mass spectrometry analysis.Type: GrantFiled: May 24, 2007Date of Patent: February 3, 2015Assignees: Brandeis University, The Brigham and Women's Hospital, Inc.Inventors: Jeffrey N. Agar, Nathalie Y. R. Agar
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Patent number: 8945485Abstract: A microfluidic valve system is disclosed that includes a matrix, a hydrophilic acceptor region a hydrophilic transfer region, and a hydrophobic gap between the acceptor region and the transfer region.Type: GrantFiled: September 24, 2012Date of Patent: February 3, 2015Assignee: Board of Governors for Higher Education, State of Rhode Island and Providence PlantationsInventors: Hong Chen, Constantine Anagnostopoulos, Mohammed Faghri, Jeremy Cogswell
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Patent number: 8936926Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.Type: GrantFiled: December 21, 2006Date of Patent: January 20, 2015Assignee: Pacific Biosciences of CaliforniaInventors: David Hanzel, Jonas Korlach, Paul Peluso, Geoffrey Otto, Thang Pham, David Rank, Stephen Turner
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Publication number: 20150017676Abstract: A capsule containing at least one mammalian cell, includes a liquid core, and at least one external envelope totally encapsulating the liquid core at its periphery, the external envelope including at least one gelled polyelectrolyte and/or a stiffened biopolymer and being able to retain the liquid core when the capsule is immersed in a gas. The present invention further relates to the method for preparing such a capsule, to a method for screening cosmetic active ingredients as well as a culture method using such capsules.Type: ApplicationFiled: January 31, 2013Publication date: January 15, 2015Applicant: CAPSUMInventors: Jerome Bibette, Nicolas Atrux-Tallau, Hugo Domejean, Annette Funfak, Nicolas Bremond, Pierre Nassoy, Kevin Allessandri
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Patent number: 8906687Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.Type: GrantFiled: June 12, 2006Date of Patent: December 9, 2014Assignee: The Invention Science Fund I, LLCInventors: Ed Harlow, Roderick A. Hyde, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
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Patent number: 8900865Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.Type: GrantFiled: March 24, 2006Date of Patent: December 2, 2014Assignee: The Invention Science Fund I, LLCInventors: Ed Harlow, Roderick A. Hyde, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
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Patent number: 8900843Abstract: The invention relates to a kit and method for the capture of tumor cells in a body fluid sample. The kit and method of the invention can capture living tumor cells but not non-living tumor cells or cell fragments so that the tumor species can be further identified by further culture of the captured tumor cells. Also, the kit and method of the invention can readily identify whether a sample contains tumor cells and collect these tumor cells for further identification so that the presence of cancer and development of the metastasis and early relapse can be found.Type: GrantFiled: March 14, 2014Date of Patent: December 2, 2014Assignee: Taipei Medical UniversityInventors: Fang-Chi Hsu, Richard Li-Chern Pan
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Patent number: 8900842Abstract: The invention relates to a method of storing and/or transporting in vitro two-dimensional cell cultures. The inventive method comprises the following steps consisting in: a) coating a cell culture that is immobilized on an asymmetric support with a gelatine solution in culture medium at a concentration of between 1 and 5%; b) solidifying the gelatine added to the support at a temperature of between 15 and 25° C.; and c) storing and/or transporting the cell culture at a temperature of between 15 and 25° C. for a period of up to 96 hours. The invention also relates to a kit which is used to store and/or transport the in vitro two-dimensional cell cultures according to the inventive method, said kit comprising: i) an asymmetric support, and (ii) a gelatine solution in culture medium at a concentration of between 1 and 5%.Type: GrantFiled: March 29, 2004Date of Patent: December 2, 2014Assignee: Advanced In Vitro Cell Technologies, S.L.Inventors: Myriam Fabre, Sonia Gonzalez Menoyo, Mariana Lopez Matas, Roser Pagan I Esquius
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Patent number: 8895279Abstract: A method to recover and harvest nutrients from a liquid stream by incorporating them into microorganisms grown in a rotating photobioreactor. The method further includes optionally integrating the rotating photobioreactor with a composting or biogenic drying process.Type: GrantFiled: September 3, 2013Date of Patent: November 25, 2014Inventor: Dennis A. Burke
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Patent number: 8895280Abstract: The present invention generally relates to improvements in enzyme immobilization, particularly for use in the field of carbon dioxide capture and sequestering. It has been discovered that the utilization of sol-gel processes to immobilize enzymes in polysilicate-polysilicone copolymer coatings and particles, and the deposition of these coatings on solid state supports or use of suspensions of these particles, provides significant benefits for use in industrial applications involving enzymatic catalysts.Type: GrantFiled: March 15, 2013Date of Patent: November 25, 2014Assignee: Akermin, Inc.Inventors: Brett M. Rambo, Tracy L. Bucholz, Dawn C. Powell, Luke E. Weber, Alexander J. Linder, Caroline M. H. Duesing, Aleksey Zaks
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Publication number: 20140342387Abstract: The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme from Agrobacterium that display improved activity when compared to the naturally occurring OpdA. The invention is also towards polypeptides that have organophosphate hydrolysing activity for the organophosphates chlorpyrifos methyl, diazinon and parathion ethyl.Type: ApplicationFiled: July 20, 2012Publication date: November 20, 2014Applicant: Commonwealth Scientific and Industrial Research OrganisationInventors: Colin Scott, John Oakeshott, Robyn Russell, Nigel French, Steven Kotsonis, Kaiyan Liu
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Publication number: 20140341982Abstract: The present invention relates to immuno-protected encapsulated cells producing an immunomodulator, for example GM-CSF (granulocyte-macrophage colony stimulating factor). The cells of the invention are particularly well adapted for providing an active adjuvant or immunomodulator, for example in the context of immunisation in humans and animals. These cells can be used for vaccination where they provide the immunomodulator in an active form, in a continuous, non-immunogenic manner in the immediate vicinity of the vaccine antigen(s). The invention also relates to a vaccine composition comprising immuno-protected encapsulated cells producing an immunomodulator and an antigenic component. The invention also relates to a kit comprising a cell as described and an antigenic component. The strategy of the invention is perfectly suited for both cancer immunotherapy and vaccination against infectious agents.Type: ApplicationFiled: March 18, 2014Publication date: November 20, 2014Inventor: Nicolas Mach
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Publication number: 20140336125Abstract: A method for improving bioactivity and/or biodegradation time of a collagen surgical implant and collagen surgical implants having such improved properties. A gas-cluster ion-beam (GCIB) is formed in a reduced-pressure chamber, a collagen surgical implant is introduced into the reduced-pressure chamber, and at least a first portion of the surface of said collagen surgical implant is irradiated with a GCIB-derived beam.Type: ApplicationFiled: December 18, 2012Publication date: November 13, 2014Inventors: Joseph Khoury, Laurence B. Tarrant, Sean R. Kirkpatrick, Richard C. Svrluga, Kshama J. Doshi
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Patent number: 8883441Abstract: A method for detecting and counting the microorganisms in a sample is described. The method comprises: a) selectively enriching the microorganism sought in the sample, b) inducing or activating at least one enzymatic activity of the microorganism, c) immunomagnetically concentrating the microorganism, d) fluorescently labeling the microorganism, and e) detecting and analyzing the fluorescence making possible the numeration or counting of the microorganisms sought by flow cytometry, filtration cytometry or fluorescence microscopy.Type: GrantFiled: October 1, 2003Date of Patent: November 11, 2014Assignee: Metis BiotechnologiesInventors: Bruno Vedrine, Aline Lachaise, Vincent Carre
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Patent number: 8883435Abstract: Described herein are irreversible kinase inhibitor compounds, methods for synthesizing such irreversible inhibitors, and methods for using such irreversible inhibitors in the treatment of diseases. Further described herein are methods, assays and systems for determining an appropriate irreversible inhibitor of a protein, including a kinase.Type: GrantFiled: March 27, 2008Date of Patent: November 11, 2014Assignee: Pharmacyclics, Inc.Inventors: Lee Honigberg, Erik Verner, Joseph J. Buggy, David Loury, Wei Chen
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Publication number: 20140329289Abstract: The invention relates to nucleic acid aptamers binding specifically to factor H, to a method for obtaining same, and to the uses thereof, in particular for the purposes of purifying factor H.Type: ApplicationFiled: November 28, 2012Publication date: November 6, 2014Applicant: Laboratoire Francais du Fractionnement et des BiotechnologiesInventors: Gerald Perret, Agnes Cibiel
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Publication number: 20140329722Abstract: Disclosed herein are methods of immobilizing a particle which comprise focusing the flow of a sample fluid containing the particle into a virtual channel which flows towards an unoccupied hydrodynamic trap in a microfluidic channel such that the particle flows into the hydrodynamic trap and becomes immobilized therein. Also disclosed are microfluidic devices which comprise at least one microchannel having at least one hydrodynamic trap, at least one focusing fluid inlet, said focusing fluid inlet is upstream of the hydrodynamic trap such that a focusing fluid introduced therein results in a virtual channel of a sample fluid when present which preferentially flows toward the hydrodynamic trap.Type: ApplicationFiled: July 15, 2014Publication date: November 6, 2014Applicant: SANDIA CORPORATIONInventors: Thomas D. Perroud, Kamlesh D. Patel
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Patent number: 8877478Abstract: This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like.Type: GrantFiled: September 21, 2007Date of Patent: November 4, 2014Assignee: Verenium CorporationInventors: Brian Steer, Alberto Alvarado, Mark Dycaico, Fatima El-Farrah, Gerhard Frey, Katie A. Kline, Arne Solbak, Tomas Todaro, Axel Trefzer
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Publication number: 20140323356Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: ApplicationFiled: May 5, 2014Publication date: October 30, 2014Applicant: BP Corporation North America Inc.Inventors: Edward J. Bylina, Ronald V. Swanson, Eric J. Mathur, David E. Lam
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Patent number: 8871266Abstract: Probiotic microorganisms are micro encapsulated by dispersing the probiotic microorganism in an aqueous suspension of a film forming protein and a carbohydrate; in an oil in water emulsion of a film forming protein and a carbohydrate and a fat; or in an oil which is subsequently dispersed in a film forming protein and a carbohydrate. The emulsion or suspension may be dried to form a powder. The probiotic may be dispersed in oil and then emulsified with the aqueous suspension and then dried to produce an encapsulated oil be dried to produce a powder. Oil suspended probiotics may be preferred where the probiotic is water sensitive. The preferred protein is casein or whey protein and the carbohydrate may be a resistant starch or a saccharide with a reducing sugar group. Where the probiotic is oxygen sensitive the protein carbohydrate is heated to create Maillard reaction products in the encapsulating film.Type: GrantFiled: March 30, 2004Date of Patent: October 28, 2014Assignee: Commonwealth Scientific & Industrial Research OrganisationInventors: Ross Crittenden, Luz Sanguansri, Mary Ann Augustin
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Patent number: 8871482Abstract: Functionalized substrate materials, for example inorganic particles and/or synthetic polymeric particles, are used to enhance bioprocesses such as saccharification and fermentation.Type: GrantFiled: May 9, 2013Date of Patent: October 28, 2014Assignee: Xyleco, Inc.Inventors: Marshall Medoff, Thomas Craig Masterman, Harrison Medoff
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Publication number: 20140315244Abstract: A fungal strain Beauveria species bearing accession number MTCC 5184 is disclosed. The process for the preparation of an enzyme mix including at least one enzyme selected from, but not limited to protease, carbohydrase, and lipase from the disclosed Beauveria species and uses of the enzyme mix in various areas also disclosed.Type: ApplicationFiled: April 10, 2014Publication date: October 23, 2014Applicant: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCHInventors: Seeta Laxman RYALI, Shiv Shankar, Snehal Vijay More, Harish Bansilal Khandelwal, Chandra Babu Kannan Narasimhan, Saravanan Palanivel, Padmanabhan Balaram
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Patent number: 8859151Abstract: Disclosed is an improved biofuel cell having a cathode comprising a dual function membrane, which contains an oxygen oxidoreductase enzyme immobilized within a buffered compartment of the membrane and an electron transport mediator which transfers electrons from an electron conducting electrode to the redox reaction catalyzed by the oxygen oxidoreductase enzyme. The improved biofuel cell also has an anode that contains an oxidoreductase enzyme that uses an organic fuel, such as alcohol, as a substrate. An electric current can flow between the anode and the cathode.Type: GrantFiled: August 31, 2004Date of Patent: October 14, 2014Assignee: St. Louis UniversityInventors: Shelley D. Minteer, Sabina Topcagic, Becky Treu
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Patent number: 8859249Abstract: A method for producing an iron (III) ion from the acidic solution containing an iodide ion and an iron (II) ion efficiently and stably is provided. The method including performing the following steps (a)-(b), repeatedly and continuously: (a) a step wherein the iron (II) ion in the acidic solution containing the iodide ion and the iron (II) ion is oxidized into iron (III) ion in a reactor using a microbes immobilizing carrier to which iron oxidizing microbes attached; (b) a step wherein sedimentation of the solution obtained in the step (a) is performed in a sedimentation tank to obtain the solution containing the iron (III) ion and concurrently the sediment of the microbes immobilizing carrier to which the iron oxidizing microbes have attached is recovered and then reintroduced into the reactor in the step (a).Type: GrantFiled: March 13, 2012Date of Patent: October 14, 2014Assignee: JX Nippon Mining & Metals CorporationInventors: Kenichi Kuwano, Akira Miura
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Patent number: 8852923Abstract: Described herein is a bioreactor system and modules capable of developing physiologically relevant fluid-induced shear stresses and regionally specific flow patterns to scaffold specimens and which can couple these stresses to cyclic flexure and/or stretch states. Methods of use of the bioreactor system and module also are provided.Type: GrantFiled: December 3, 2010Date of Patent: October 7, 2014Assignee: University of Pittsburgh—Of the Commonwealth System of Higher EducationInventors: Steven M. Boronyak, George C. Engelmayr, Jr., Sharan Ramaswamy, Michael S. Sacks, David E. Schmidt, Mohammed S. El-Kurdi
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Patent number: 8852881Abstract: A device for characterizing the biological properties of cells can include a plurality of dual-compartment assay chambers wherein the compartments of each chamber are separated by a cell layer across which ions can flow. The biological properties of the cell layer in the presence or absence of experimental compounds can be determined by measuring an electrical gradient across the layer. A individual dual-compartment chamber of this type may be referred to as an “Ussing chamber.Type: GrantFiled: November 29, 2006Date of Patent: October 7, 2014Assignee: Vertex Pharmaceuticals, Inc.Inventors: Paul A. Negulescu, Alec T. Harootunian, Philip E. Salzmann, Javier H. Flores, James E. Sinclair, Minh Vuong, Ashvani K. Singh, Fred F. VanGoor
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Patent number: 8852917Abstract: The present invention relates to a new bacteriocin, to microbial strains which can produce it and to uses of the bacteriocin and the strains. The bacteriocin is effective against Clostridium difficile and Listeria monocytogenes amongst other organisms.Type: GrantFiled: November 28, 2008Date of Patent: October 7, 2014Assignees: University College Cork, TEAGASC, The Agriculture and Food Development AuthorityInventors: Colin Hill, Mary Rea, Paul Ross
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Patent number: 8852932Abstract: A method of making engineered tissue from a plurality of cell aggregates is disclosed. A cell suspension is centrifuged. The resulting pellet is extruded through an orifice, and the extruded pellet is cut into pieces to produce cell aggregates. A plurality of the cell aggregates are printed in a pattern, and allowed to fuse to form a desired three-dimensional engineered tissue structure. Modeling methods predict the structural evolution of fusing cell aggregates for combinations of cell type to enable selection of organ printing process parameters for use in producing an engineered tissue having a desired three-dimensional structure.Type: GrantFiled: June 21, 2012Date of Patent: October 7, 2014Assignees: The Curators of the University of Missouri, Medical University of South Carolina, MUSC Foundation for Research DevelopmentInventors: Gabor Forgacs, Karoly Jakab, Adrian Neagu, Vladimir Mironov
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Publication number: 20140296111Abstract: A protein-immobilizing solid phase is a protein-immobilizing solid phase comprising an mRNA-nucleic acid linker-protein complex, obtained by linking the mRNA and the protein encoded by that mRNA through the nucleic acid linker, immobilized on the solid phase, wherein the nucleic acid linker has a photocleavage site and a solid phase binding site.Type: ApplicationFiled: April 30, 2014Publication date: October 2, 2014Applicants: Nikon Corporation, The University of TokyoInventors: Shingo Ueno, Naoto Nemoto, Takanori Ichiki, Hirofumi Shiono, Hisao Osawa
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Publication number: 20140298499Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: December 16, 2013Publication date: October 2, 2014Applicant: BP Corporation North America Inc.Inventors: Kevin A. Gray, Reinhard Dirmeier
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Publication number: 20140273142Abstract: Provided herein are nanolipoprotein particles that comprise a biosynthetic enzyme more particularly an enzyme capable of catalyzing rubber or other rubbers polymerization, and related assemblies, devices, methods and systems.Type: ApplicationFiled: March 6, 2014Publication date: September 18, 2014Applicant: LAWRENCE LIVERMORE NATIONAL SECURITY, LLCInventor: Paul D. HOEPRICH
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Patent number: 8835143Abstract: The invention relates to a method for the preparation of hybrid cross-linked enzyme-silica aggregates including the steps of taking up enzyme molecules in a solvent, precipitating the enzyme molecules using a precipitation agent, and adding an alkoxysilane and crosslinking the mixture of alkoxysilane and precipitated enzyme aggregates, using a crosslinking agent comprising an aldehyde, to obtain hybrid crosslinked enzyme-silica aggregates.Type: GrantFiled: June 16, 2011Date of Patent: September 16, 2014Assignee: Clea Technologies BVInventors: Willem Robert Klaas Schoevaart, Lukas Michael Van Langen, Ronald Tako Marinus van Den Dool, Johannes Wilhelmus Leonardus Boumans
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Patent number: 8822190Abstract: The present invention relates to eco-friendly compositions and methods for providing plant growth enhancing formulations comprising mixtures of microbial isolates. In particular, numerous bacterial and fungal strains were isolated from a variety of soil types, from rhizospheres and from root nodules of leguminous plants, in designed combinations, for providing plant growth and plant productivity enhancing formulations. These specifically designed polymicrobial formulations would further provide protection against plant pathogens lowering the need for nitrogen containing fertilizers, solubilize minerals, protect plants against pathogens, and make available to the plant valuable nutrients, such as phosphate, thus reducing and eliminating the need for using chemical fertilizers and chemical pesticides.Type: GrantFiled: January 15, 2009Date of Patent: September 2, 2014Assignee: Board of Trustees of Michigan State UniversityInventors: C. Adinarayana Reddy, Lalithakumari Janarthanam
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Patent number: 8822170Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.Type: GrantFiled: August 19, 2011Date of Patent: September 2, 2014Assignee: Promega CorporationInventors: Keith V. Wood, Monika G. Wood, Mary P. Hall
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Patent number: 8815584Abstract: The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture.Type: GrantFiled: April 23, 2010Date of Patent: August 26, 2014Assignee: University of Central Florida Research Foundation, Inc.Inventors: James J. Hickman, Mainak Das
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Patent number: 8815556Abstract: In accordance with certain embodiments of the present disclosure, a kit is described. The kit includes primed living cells joined to and at least partially within a three-dimensional hydrogel structure and an isolated polypeptide having the carboxy-terminal amino acid sequence of an alpha Connexin, or a conservative variant thereof, wherein the polypeptide does not include the full length alpha Connexin protein.Type: GrantFiled: April 1, 2013Date of Patent: August 26, 2014Inventors: Robert G. Gourdie, Jay D. Potts
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Patent number: 8807879Abstract: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.Type: GrantFiled: September 14, 2012Date of Patent: August 19, 2014Assignee: The General Hospital CorporationInventors: Mehmet Toner, Dino Dicarlo, Jon F. Edd, Daniel Irimia