Ribonuclease (3.1.4) Patents (Class 435/199)
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Publication number: 20150079064Abstract: Disclosed herein are transcription activator-like effector nuclease (TALEN)-related compositions and methods of using said TALENs for correcting mutant genes.Type: ApplicationFiled: April 26, 2013Publication date: March 19, 2015Applicant: Duke UniversityInventors: Charles Gersbach, David Ousterout
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Patent number: 8969061Abstract: Compositions, methods and a kit are described relating to a novel family of enzymes which preferentially bind to a hydroxymethylated cytosine or a glucosylated hydroxymethylated cytosine and cleave double-stranded DNA at a defined distance 3? of the recognition site to produce a cleavage fragment with a 1-3 base overhang.Type: GrantFiled: January 20, 2011Date of Patent: March 3, 2015Assignee: New England Biolabs, Inc.Inventors: Zhenyu Zhu, Yu Zheng, Shengxi Guan, Hua Wang, Aine Quimby, Penghua Zhang, Lynne Apone
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Publication number: 20150040252Abstract: The invention relates to isolation of novel ?-actin and ribosomal protein S21 (rpS21) promoters and uses thereof. In particular, this invention features nucleotide sequences for rodent ?-actin promoters including, hamster, rat, and mouse, and hamster rpS21 promoter.Type: ApplicationFiled: August 1, 2014Publication date: February 5, 2015Applicant: GENZYME CORPORATIONInventors: Scott D. ESTES, Weiqun ZHANG
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Publication number: 20150037842Abstract: The disclosure relates to a Gram negative bacterial cell that is transformed with a nucleic acid molecule that encodes a Gram positive twin-arginine translocase and including methods for the production of polypeptides.Type: ApplicationFiled: February 22, 2013Publication date: February 5, 2015Inventor: Colin Robinson
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Patent number: 8945839Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.Type: GrantFiled: April 18, 2014Date of Patent: February 3, 2015Assignees: The Broad Institute Inc., Massachusetts Institute of TechnologyInventor: Feng Zhang
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Publication number: 20150017728Abstract: The present invention provides compositions and methods for targeted cleavage of cellular chromatin in a region of interest and/or homologous recombination at a predetermined site in cells. Compositions include fusion polypeptides comprising a TAL effector binding or a zinc finger domain and an I-TevI homing endonuclease cleavage domain as well as nucleic acid sequence encoding the same. The use of the I-TevI domain allows for monomer endonuclease sequences to achieve cleavage of cellular chromatin and represents an advantage over prior endonucleases which require self-dimerization, and two nucleases with appropriate spacers.Type: ApplicationFiled: September 19, 2012Publication date: January 15, 2015Inventors: Bing Yang, Ting Li, Sheng Huang
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Patent number: 8932814Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.Type: GrantFiled: April 22, 2014Date of Patent: January 13, 2015Assignees: The Broad Institute Inc., Massachusetts Institute of Technology, President and Fellows of Harvard CollegeInventors: Le Cong, Feng Zhang
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Publication number: 20150010982Abstract: The present invention concerns methods and compositions for making and using bioactive assemblies of defined compositions, which may have multiple functionalities and/or binding specificities. In particular embodiments, the bioactive assembly is formed using dock-and-lock (DNL) methodology, which takes advantage of the specific binding interaction between dimerization and docking domains (DDD) and anchoring domains (AD) to form the assembly. In various embodiments, one or more effectors may be attached to a DDD or AD sequence. Complementary AD or DDD sequences may be attached to an adaptor module that forms the core of the bioactive assembly, allowing formation of the assembly through the specific DDD/AD binding interactions. Such assemblies may be attached to a wide variety of effector moieties for treatment, detection and/or diagnosis of a disease, pathogen infection or other medical or veterinary condition.Type: ApplicationFiled: September 17, 2014Publication date: January 8, 2015Inventors: Chien-Hsing Chang, David M. Goldenberg, William J. McBride, Edmund A. Rossi
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Patent number: 8911948Abstract: The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3? end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.Type: GrantFiled: July 22, 2009Date of Patent: December 16, 2014Assignee: Integrated DNA Technologies, Inc.Inventors: Joseph Alan Walder, Mark Aaron Behlke, Scott Rose, Joseph Dobosy
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Publication number: 20140363561Abstract: The invention relates to methods for stably integrating a desired polynucleotide into a plant genome.Type: ApplicationFiled: March 14, 2014Publication date: December 11, 2014Applicant: J.R. SIMPLOT COMPANYInventors: CAIUS M. ROMMENS, HUI DUAN, J. TROY WEEKS
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Publication number: 20140356960Abstract: The present invention relates to a nucleic acid containing at least one homing endonuclease site (HE) and at least one restriction enzyme site (X) wherein the HE and X sites are selected such that HE and X result in compatible cohesive ends when cut by the homing endonuclease and restriction enzyme, respectively, and the ligation product of HE and X cohesive ends can neither be cleaved by the homing endonuclease nor by the restriction enzyme. Further subject-matter of the present invention relates to a vector comprising the nucleic acid of the present invention, host cells containing the nucleic acid and/or the vector; a kit for cloning and/or expression of multiprotein complexes making use of the vector and the host cells, a method for producing a vector containing multiple expression cassettes, and a method for producing multiprotein complexes.Type: ApplicationFiled: April 25, 2014Publication date: December 4, 2014Inventor: Imre Berger
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Publication number: 20140359798Abstract: Genetically engineered mice having a corrected Cbr1rd8 mutation and methods for correcting the Cbr1rd8 mutation to produce the genetically engineered mice are provided according to aspects of the present invention. Kits, expression vectors and fusion proteins according to aspects of the invention are provided for use to produce the genetically engineered mice characterized by a corrected Cbr1rd8 mutation.Type: ApplicationFiled: June 3, 2014Publication date: December 4, 2014Applicant: THE JACKSON LABORATORYInventors: Michael V. Wiles, Benjamin E. Low
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Publication number: 20140342400Abstract: The present invention relates to an isolated peptide consisting of the amino acid sequence of SEQ ID NO: 1. The present invention also relates to a method for increasing expression of a target protein using the peptide consisting of the amino acid sequence of SEQ ID NO: 1, comprising (a) fusing the peptide with the target protein to form a recombinant protein; and (b) expressing the recombinant protein by an expression host.Type: ApplicationFiled: May 13, 2014Publication date: November 20, 2014Applicant: NATIONAL TSING HUA UNIVERSITYInventor: Margaret Dah-Tsyr Chang
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Patent number: 8889394Abstract: Described herein are methods and compositions for generating and using fusion proteins.Type: GrantFiled: September 7, 2009Date of Patent: November 18, 2014Assignee: Empire Technology Development LLCInventor: Sreekanth H. Chalasani
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Patent number: 8889356Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.Type: GrantFiled: February 18, 2014Date of Patent: November 18, 2014Assignees: The Broad Institute Inc., Massachusetts Institute of TechnologyInventor: Feng Zhang
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Publication number: 20140335593Abstract: This invention relates to altered forms of members of the RNase A superfamily. An RNase A can be modified to be cytotoxic by altering its amino acid sequence so that it is not bound easily by the ribonuclease inhibitor while still retaining catalytic properties. While earlier work had identified some modifications to RNase A that would result in cytotoxicity, the use of the FADE algorithm for molecular interaction analysis has led to several other locations that were candidates for modification. Some of those modifications did result in RNase A variants with increase cytotoxicity.Type: ApplicationFiled: July 18, 2014Publication date: November 13, 2014Inventors: Ronald Raines, Julie C. Mitchell, Thomas J. Rutkoski
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Publication number: 20140329233Abstract: Methods and kits for joining two or more polynucleotides to form a product polynucleotide are provided. A mixture contains a first polynucleotide comprising a selectable marker. The mixture further contains a second polynucleotide comprising a first typeIIs recognition sequence and a second typeIIs recognition sequence. The second polynucleotide is other than the first polynucleotide. The mixture further contains a first typeIIs restriction endonuclease that cleaves the first typeIIs recognition sequence to produce a first end, a second typeIIs restriction endonuclease that cleaves the second typeIIs recognition sequence to produce a second end, and a DNA ligase. The first end is not compatible with the second end. The combined actions of the enzymes in the mixture join the first polynucleotide to the second polynucleotide forming a product polynucleotide, which is obtained by transforming the mixture into a host cell.Type: ApplicationFiled: May 1, 2013Publication date: November 6, 2014Applicant: DNA Twopointo, Inc.Inventors: Jeremy Minshull, Jon Ness, Elias Theodorou
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Patent number: 8871445Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.Type: GrantFiled: April 23, 2014Date of Patent: October 28, 2014Assignees: The Broad Institute Inc., Massachusetts Institute of Technology, President and Fellows of Harvard CollegeInventors: Le Cong, Feng Zhang
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Patent number: 8865406Abstract: The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are compositions and methods related to components of a CRISPR complex particularly comprising a Cas ortholog enzyme.Type: GrantFiled: March 24, 2014Date of Patent: October 21, 2014Assignees: The Broad Institute Inc., Massachusetts Institute of TechnologyInventors: Feng Zhang, Fei Ran
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Patent number: 8859266Abstract: The present disclosure encompasses systems, and their methods of use, for detecting a target analyte. The systems include a first and second oligonucleotide probe that associate together to form a complex that binds to a target analyte; a cleavable reporter molecule that binds to the complex; and cleaving agent.Type: GrantFiled: April 28, 2011Date of Patent: October 14, 2014Assignee: University of Central Florida Research Foundation, Inc.Inventors: Yuliva V. Gerasimova, Dmitry M. Kolpashchikov
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Publication number: 20140302563Abstract: The present disclosure provides nucleic acids encoding an RNA recognition sequence positioned proximal to an insertion site for the insertion of a sequence of interest; and host cells genetically modified with the nucleic acids. The present disclosure also provides methods of modifying the activity of a target RNA, and kits and compositions for carrying out the methods.Type: ApplicationFiled: April 9, 2014Publication date: October 9, 2014Applicant: The Regents of the University of CaliforniaInventors: Jennifer A. Doudna, Lei S. Qi, Rachel E. Haurwitz, Adam P. Arkin
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Patent number: 8852911Abstract: The present disclosure provides a method for producing a Dicer polypeptide in a prokaryotic host cell. The present disclosure further provides a purified Dicer complex. The present disclosure further provides kits for producing a Dicer polypeptide in a prokaryotic host cell.Type: GrantFiled: August 2, 2012Date of Patent: October 7, 2014Assignee: The Regents of the University of CaliforniaInventors: Jennifer A. Doudna, Enbo Ma
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Publication number: 20140294758Abstract: Provided are recombinant antibodies comprising one or more peptides fused to the C-terminus of the light chain constant region. Recombinant immunocytokines comprising a cytokine fused to the C-terminus of the light chain constant region are described and shown to be surprisingly active.Type: ApplicationFiled: June 24, 2012Publication date: October 2, 2014Inventor: Stephen D. Gillies
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Publication number: 20140294801Abstract: The present invention provides an improved and specific mRNA interferase and related methods of protein-based mRNA interference.Type: ApplicationFiled: August 3, 2012Publication date: October 2, 2014Applicant: Rutgers, The State University of New JerseyInventors: Masayori Inouye, Yoshihiro Yamaguchi
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Publication number: 20140295493Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: April 1, 2014Publication date: October 2, 2014Inventors: Hiroaki Udagawa, Christian Isak Jorgensen
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Publication number: 20140295556Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.Type: ApplicationFiled: March 14, 2014Publication date: October 2, 2014Applicant: THE GENERAL HOSPITAL CORPORATIONInventors: J. Keith Joung, Shengdar Tsai
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Patent number: 8840882Abstract: The present invention relates generally to conjugates of human ribonucleases and water-soluble polymers, compositions comprising the conjugates and methods of using the same. In particular, the present invention provides conjugates of human ribonucleases and one or more water-soluble polymer compositions (e.g., to increase serum half-life and a pharmacokinetic profile, in vivo biological activity, stability, and/or reduce host immune response to the protein in vivo) as well as methods of using the conjugates in the therapy, treatment, and/or prevention of disease (e.g., cancer).Type: GrantFiled: June 25, 2007Date of Patent: September 23, 2014Assignee: Quintessence Biosciences, Inc.Inventors: John A. Kink, Laura E. Strong, Vladimir Trubetskoy, Mark N. Shahan
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Publication number: 20140273234Abstract: The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are compositions and methods related to components of a CRISPR complex particularly comprising a Cas ortholog enzyme.Type: ApplicationFiled: June 2, 2014Publication date: September 18, 2014Applicants: The Board Institute, Inc., Massachusetts Institute of TechnologyInventors: Feng ZHANG, Fei RAN
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Publication number: 20140255925Abstract: The invention provides a provides improvements to assays that employ RNase H cleavage for biological applications related to nucleic acid amplification and detection, where the RNase H has been reversibly inactivated.Type: ApplicationFiled: March 15, 2013Publication date: September 11, 2014Applicant: Integrated DNA TechnologiesInventors: Joseph Alan WALDER, Mark Aaron BEHLKE, Scott D. ROSE, Joseph DOBOSY, Susan Marie RUPP
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Patent number: 8828698Abstract: Stabilized angiogenin compositions and methods of preparing a stabilized angiogenin compositions by covalent immobilization on a naturally occurring substrate, such as a polysaccharide substrate, are disclosed. In particular, the polysaccharide substrate includes galactose-rich polysaccharide.Type: GrantFiled: April 12, 2007Date of Patent: September 9, 2014Assignee: Naidu LPInventor: A. Satyanarayan Naidu
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Publication number: 20140249094Abstract: An object of the present invention is to provide a safety sensation-improving agent that can improve dulled peripheral sensations through daily ingestion or application to the skin. Another object of the present invention is to provide a sensation-improving food, beverage, feed, or cosmetics that can improve dulled peripheral sensations through oral ingestion or application to the skin. A sensation-improving agent containing a milk-derived protein and/or a hydrolysate therefrom as an active ingredient is provided. The milk-derived protein and/or the hydrolysate therefrom can be orally ingested or applied direct to the skin to improve dulled sensations, particularly peripheral sensations, and be formed into a sensation-improving food, beverage, feed, or cosmetics.Type: ApplicationFiled: October 2, 2012Publication date: September 4, 2014Inventors: Ken Katoh, Hiroshi Ueno, Yuko Ono, Noriko Ueda, Toshiya Kobayashi, Takahiro Moriya, Yutaro Obata
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Publication number: 20140242702Abstract: Methods and compositions for increasing nuclease-mediated genomic modification using DNA repair inhibitors are provided.Type: ApplicationFiled: February 24, 2014Publication date: August 28, 2014Inventors: Fuqiang Chen, Qiaohua Kang, Thomas Wechsler
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Publication number: 20140234975Abstract: The present invention relates to a method for increasing double-strand-break induced mutagenesis at a genomic locus of interest in a cell, thereby giving new tools for genome engineering, including therapeutic applications and cell line engineering. More specifically, the present invention concerns the combined use of TALEN or meganucleases with TREX2, especially under the form of single-chain proteins.Type: ApplicationFiled: July 3, 2012Publication date: August 21, 2014Applicant: CELLECTISInventors: George H Silva, Rachel Macmaster
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Publication number: 20140227244Abstract: The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.Type: ApplicationFiled: February 5, 2014Publication date: August 14, 2014Inventors: Andrew M. Scharenberg, Michael T. Certo, Kamila Sabina Gwiazda
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Publication number: 20140227787Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.Type: ApplicationFiled: April 18, 2014Publication date: August 14, 2014Applicants: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGYInventor: Feng Zhang
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Patent number: 8795965Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.Type: GrantFiled: February 18, 2014Date of Patent: August 5, 2014Assignees: The Broad Institute, Inc., Massachusetts Institute of TechnologyInventor: Feng Zhang
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Publication number: 20140212928Abstract: Disclosed herein are methods and compositions for generating a single-stranded break in a target sequence, which facilitates targeted integration of one or more exogenous sequences.Type: ApplicationFiled: March 6, 2014Publication date: July 31, 2014Applicant: Sangamo BioSciences, Inc.Inventor: Jianbin Wang
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Publication number: 20140212946Abstract: The present invention is directed toward the delivery of a toxic protein to pathogenic cells, particularly cancer cells. In preferred embodiments, the toxic protein is a ribonuclease that has been modified to make it toxic to target cells and that can be conjugated to a target cell-specific delivery vector, such as an antibody, for delivery to pathogenic cells.Type: ApplicationFiled: April 14, 2014Publication date: July 31, 2014Applicant: Quintessence Biosciences, Inc.Inventors: Laura E. Strong, Peter A. Leland, Thomas Burke
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Publication number: 20140212871Abstract: Methods for extracting high quality nucleic acids from a heterogenous collection of nucleic acid-containing materials from a biological sample are disclosed. The heterogenous collection of nucleic-acid containing materials may contain cells or microvesicles, or both. The extractions obtained by the methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis, or therapy evaluation for a medical condition.Type: ApplicationFiled: May 11, 2012Publication date: July 31, 2014Applicant: EXOSOME DIAGNOSTICS, INC.Inventors: Wayne Comper, Leileata M. Russo, Johan Karl Olov Skog
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Publication number: 20140208457Abstract: The present invention is concerned with nuclease fusion proteins and various uses thereof. Specifically, it relates to a polynucleotide encoding a polypeptide comprising (i) a first module comprising at least a first DNA binding domain derived from a homing endonuclease, (ii) a linker, and (iii) a second module comprising at least a second DNA binding domain and a cleavage domain derived from a restriction endonuclease, wherein said polypeptide functionally interacts only with DNA comprising a DNA recognition site for the first DNA binding domain and a DNA recognition site for the second DNA binding domain, and wherein said cleavage domain cleaves DNA within a specific DNA cleavage site upon binding of the polypeptide. Further contemplated are a vector and a non-human transgenic organism comprising said polynucleotide as well as a polypeptide encoded by the polynucleotide of the invention.Type: ApplicationFiled: June 8, 2012Publication date: July 24, 2014Applicant: BASF PLANT SCIENCE COMPANY GMBHInventors: Ines Fonfara, Wolfgang Wende, Alfred Pingoud
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Publication number: 20140194299Abstract: Compositions, methods and related uses are provided relating to cleaving modified DNA. For example, a set of DNA fragments obtainable by enzymatic cleavage of a large DNA is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide. In addition, an enzyme preparation is provided that includes one or more enzymes that recognize a modified nucleotide in a DNA and cleave the DNA at a site that is at a non-random distance from the modified nucleotide. The one or more enzymes are further characterized by an N-terminal conserved domain with greater than 90% amino acid sequence homology to WXD(X)10YXGD. The related uses include creating a methylome, methods of purifying DNA fragments containing a modified nucleotide and diagnostic applications.Type: ApplicationFiled: January 30, 2014Publication date: July 10, 2014Applicant: New England Biolabs, Inc.Inventors: YU ZHENG, RICHARD J. ROBERTS
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Patent number: 8771982Abstract: The present invention relates, in general, to telomeres, and, in particular, to a method or effecting telomere elongation in mammalian cells. The invention further relates to a protein suitable for use in such a method and to nucleic acid sequences encoding same.Type: GrantFiled: April 30, 2004Date of Patent: July 8, 2014Assignee: Duke UniversityInventors: Christopher M. Counter, Blaine N. Armbruster
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Patent number: 8771945Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.Type: GrantFiled: February 18, 2014Date of Patent: July 8, 2014Assignees: The Broad Institute, Inc., Massachusetts Institute of TechnologyInventor: Feng Zhang
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Publication number: 20140186895Abstract: The subject of the invention is a peptide with the enzymatic activity of a Dicer-like protein, a method for preparing short RNA molecules, and use thereof. The purpose of the solution was to develop a new method of producing short RNA molecules, using a new, MtDCL1pepA peptide of a Dicer protein activity designed by inventors.Type: ApplicationFiled: June 25, 2012Publication date: July 3, 2014Applicant: INSTYTUT CHEMII BIOORGANICZNEJ PANInventors: Marek Figlerowicz, Aleksander Tworak, Jan Podkowinski, Natalia Koralewska, Anna Kurzynska-Kokorniak
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Publication number: 20140179005Abstract: Methods of inserting genes into defined locations in the chromosomal DNA of cultured mammalian cell lines which are subject to gene amplification are disclosed. In particular, sequences of interest (e.g., genes encoding biotherapeutic proteins) are inserted proximal to selectable genes in amplifiable loci, and the transformed cells are subjected to selection to induce co-amplification of the selectable gene and the sequence of interest. The invention also relates to meganucleases, vectors and engineered cell lines necessary for performing the methods, to cell lines resulting from the application of the methods, and use of the cell lines to produce protein products of interest.Type: ApplicationFiled: November 27, 2013Publication date: June 26, 2014Applicant: PRECISION BIOSCIENCES, INC.Inventors: Derek JANTZ, James Jefferson SMITH, Michael G. NICHOLSON
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Publication number: 20140178353Abstract: An isolated polypeptide is disclosed comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 2773-5544 and 11089-11094, wherein the polypeptide has antimicrobial activity. Uses thereof for treating microbial infections are also disclosed.Type: ApplicationFiled: December 26, 2013Publication date: June 26, 2014Applicant: Yeda Research and Development Co., Ltd.Inventors: Rotem SOREK, Hila Sberro, Azita Leavitt
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Publication number: 20140163084Abstract: Based at least in part on an understanding of the mechanisms by which small RNAs (e.g., naturally-occurring miRNAs) mediate RNA silencing in plants, rules have been established for determining, for example, the degree of complementarity required between an RNAi-mediating agent and its target, i.e., whether mismatches are tolerated, the number of mismatches tolerated, the effect of the position of the mismatches, etc. Such rules are useful, in particular, in the design of improved RNAi-mediating agents which allow for more exact control of the efficacy of RNA silencing.Type: ApplicationFiled: June 13, 2013Publication date: June 12, 2014Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Phillip D. ZAMORE, Guiliang TANG
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Patent number: 8748698Abstract: A genome shuffling method for autogamous plants, including producing individuals having the following three traits in a tight coupling linkage by a gene engineering technique selected from a transgenic technique and a gene targeting technique: 1) dominant male sterility, 2) chemical tolerance and 3) lethality inducible by activating an inducible promoter, selecting, from progeny of the individuals, male-sterile individuals by means of the chemical tolerance described in 2) and male-fertile individuals by means of the lethality described in 3), arranging the male-sterile individuals and the male-fertile individuals close together in flowering periods thereof, so that the male-sterile individuals are crossed with the male-fertile individuals, harvesting seeds from the male-sterile individuals, and repeating outcrossing using the seeds from generation to generation.Type: GrantFiled: December 10, 2013Date of Patent: June 10, 2014Inventor: Junichi Tanaka
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Patent number: 8748146Abstract: Aspects of the invention provide engineered endonucleases that are characterized by both a long recognition sequence and specific cleavage outside of the recognition site. Engineered endonucleases of the invention are useful for manipulating long pieces of DNA.Type: GrantFiled: April 19, 2008Date of Patent: June 10, 2014Assignee: Celexion, LLCInventors: Shaun Lippow, Dasa Lipovsek, Patricia M. Aha
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Publication number: 20140157458Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.Type: ApplicationFiled: November 25, 2013Publication date: June 5, 2014Applicants: Sangamo BioSciences, Inc., Dow AgroSciences LLCInventors: Russell DeKelver, Manju Gupta, Jeffrey C. Miller, Stephen Novak, Joseph F. Petolino