Fungal Source Patents (Class 435/203)
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Patent number: 6982163Abstract: A process for preparing beta-fructofuranosidase enzyme and a process for producing fructooligosaccharides, in which the preparation of the enzyme is obtained by cultivating the fungus Aspergillus niger, either wild or mutated, in a preferably semi-solid culture medium, in order to produce an extracellular enzyme, which is submitted to transfructosylation for producing fructooligosaccharides comprising sugars which are formed by one unit of sacrose and by two, three and four units of fructose.Type: GrantFiled: September 30, 2002Date of Patent: January 3, 2006Assignee: Usina Da Barra S/A-Acucar e AlcoolInventors: Yong Kun Park, Glaucia Maria Pastores
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Patent number: 6916645Abstract: Liquefying alkaline amylases, each having residual activity not less than 70% when treated at pH 10 and 45° C. for 30 minutes in the presence of 1 to 100 mM of EDTA or EGTA, are described. Also described are detergents comprising these amylases. In comparison with conventional amylases for detergents, the liquefying alkaline amylases of this invention have a high chelating-agent resisting performance.Type: GrantFiled: May 2, 2002Date of Patent: July 12, 2005Assignee: Kao CorporationInventors: Hiroshi Hagihara, Kaori Kitayama, Yasuhiro Hayashi, Kazuaki Igarashi, Keiji Endo, Katsuya Ozaki
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Patent number: 6900039Abstract: The properties of dough or bread can be improved by the addition of a carbohydrate oxidase which can oxidize the reducing end of an oligosaccharide more efficiently than the corresponding monosaccharide, e.g., preferentially oxidizing maltodextrins or cellodextrins over glucose. A novel carbohydrate oxidase having the capability to oxidize maltodextrins and cellodextrins more efficiently than glucose may be obtained from a strain of Microdochium, particularly M. nivale. The amino acid sequence of the novel carbohydrate oxidase has very low homology (<20% identity) with known amino acid sequences.Type: GrantFiled: December 6, 2002Date of Patent: May 31, 2005Assignee: Novozymes A/SInventors: Palle Schneider, Søren Christensen, Lone Dybdal, Claus Crone Fuglsang, Feng Xu, Elizabeth Golightly
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Patent number: 6846656Abstract: The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.Type: GrantFiled: August 25, 2000Date of Patent: January 25, 2005Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Satoshi Koizumi, Kazuhiko Tabata, Tetsuo Endo, Akio Ozaki
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Publication number: 20040229764Abstract: The invention relates to a variant of a parent Fungamyl-like fungal alpha-amylase, which exhibits improved thermal stability at acidic pH suitable for, e.g., starch processes.Type: ApplicationFiled: April 7, 2004Publication date: November 18, 2004Applicant: Novozymes A/SInventors: Henrik Bisgard-Frantzen, Allan Svendsen, Sven Pedersen
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Patent number: 6815191Abstract: An endo-&bgr;-N-acetylglucosaminidase gene encoding the following protein (a) or (b): (a) a protein comprising the amino acid sequence represented by SEQ ID NO: 3; and (b) a protein comprising an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 3 by deletion, substitution, or addition of at least one amino acid and having the activity of endo-&bgr;-N-acetylglucosaminidase.Type: GrantFiled: July 20, 2001Date of Patent: November 9, 2004Assignee: Kirin Beer Kabushiki KaishaInventors: Kazuo Kobayashi, Makoto Takeuchi, Akihiko Iwamatsu, Kenji Yamamoto, Hidehiko Kumagai, Satoshi Yoshida
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Publication number: 20040142434Abstract: The invention relates to starch conversion processes using glucoamylases derived from Talaromyces emersonii and related glucoamylases.Type: ApplicationFiled: July 22, 2003Publication date: July 22, 2004Applicant: Novozymes A/SInventors: Bjarne Ronfeldt Nielsen, Ruby Ilum Nielsen, Jan Lehmbeck
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Patent number: 6743615Abstract: The present invention is a process for converting trimethylhydroquinone diacetate (TMHQ-DA) into trimethylhydroquinone-1-monoacetate (TMHQ-1-MA) by contacting TMHQ-DA with a lipase to effect an enzymatic monosaponification of the TMHQ-DA. Also provided are methods of making (all-rac)-&agr;-tocopherol and (all-rac)-&agr;-tocopherol acetate.Type: GrantFiled: February 19, 2002Date of Patent: June 1, 2004Assignee: Roche Vitamins, Inc.Inventors: Werner Bonrath, Detlef Eisenkraetzer, Valerie Enjolras, Reinhard Karge, Thomas Netscher, Michael Schneider
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Patent number: 6723840Abstract: The invention provides isolated nucleic acid and amino acid sequences of TL-&ggr;, antibodies to TL-&ggr;, methods of screening for TL-&ggr; modulators using biologically active TL-&ggr;, and kits for screening for TL-&ggr; modulators.Type: GrantFiled: November 28, 2000Date of Patent: April 20, 2004Assignee: The Regents of the University of CaliforniaInventors: Roman Sakowicz, Lawrence S. B. Goldstein
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Patent number: 6677137Abstract: Avian and reptile derived heparanase and nucleic acids encoding same.Type: GrantFiled: August 16, 2001Date of Patent: January 13, 2004Assignees: Insight Strategy & Marketing Ltd., Hadesit Medical Research Services and Development Ltd.Inventors: Orit Goldshmidt, Iris Pecker, Israel Vlodavsky, Israel Michal, Eyal Zcharia
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Publication number: 20030148500Abstract: The present invention relates to a transcription factor found in filamentous fungi, especially in Aspergillii, DNA sequences coding for said factor, its transformation into and expression in fungal host organisms, and the use of said factor in such hosts for increasing the expression of a polypeptide of interest being produced by said host.Type: ApplicationFiled: February 20, 2003Publication date: August 7, 2003Applicant: Novozymes A/SInventor: Tove Christensen
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Patent number: 6586209Abstract: An isolated DNA with a nucleotide sequence encoding a ripening form of a xylanase fungal origin having bread improving activity. Cells are transformed with this DNA and used to produce the ripening form of xylanase which itself is suitable for use in flour and dough.Type: GrantFiled: February 18, 1993Date of Patent: July 1, 2003Assignee: Quest International, B.V.Inventors: Robert F. M. van Gorcom, Johanna G. M. Hessing, Jan Maat, Martinus Roza, Johannes Maria A. Verbakel
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Patent number: 6566114Abstract: Novel mannanases comprising e.g. an amino acid sequence as shown in positions 31-330 of SEQ ID NO:2 or their homologues may be derived from e.g. Bacillus sp. I633, or may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO: 1 from nucleotide 91 to nucleotide 990, polynucleotide molecules that encode a polypeptide that is at least 65% identical to the amino acid sequence of SEQ ID NO: 2 from amino acid residue 31 to amino acid residue 330, or degenerate nucleotide sequences thereof. The mannanases are alkaline and are useful e.g. in cleaning compositions, in a fracturing fluid useful to fracture a subterranean formation, for modifying plant material, and for treatment of cellulosic fibers.Type: GrantFiled: June 24, 1999Date of Patent: May 20, 2003Assignee: Novozymes, A/SInventors: Markus Sakari Kauppinen, Martin Schülein, Kirk Schnorr, Lene Nonboe Andersen, Mads Eskelund Bjørnvad
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Patent number: 6558728Abstract: Recombinantly produced &agr;-glucuronidases which are useful in food manufacturing and as a feed additive to enhance the utilization of the feed components, and in other industrial applications such as pulp processing are provided. Genes coding for such enzymes can e.g. be isolated from Aspergillus sp. including A. tubigensis or A. niger.Type: GrantFiled: November 10, 1998Date of Patent: May 6, 2003Assignee: Danisco A/SInventors: Charlotte Horsmans Poulsen, Masoud R. Zargahi, Ronald Peter de Vries, Jacob Visser
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Patent number: 6559131Abstract: The invention concerns the use of fucanes for obtaining medicines for modulating metalloprotease and inhibiting leukocytic elastase. Said medicines help activate collagen synthesis inhibit proliferation of gingival fibroblasts, and activate proliferation of dermal fibroblasts. They are useful in particular for treating periodontal pathologies and dermal lesions.Type: GrantFiled: November 6, 2000Date of Patent: May 6, 2003Assignees: Ifremer, Centre National de la Recherche Scientifique, Universite Rene DescartesInventors: Karim Senni, Bernard Pellat, Bruno Gogly, Catherine Blondin, Didier Letourneur, Jacqueline Jozefonvicz, Corinne Sinquin, Sylvia Colliec-Jouault, Patrick Durand
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Patent number: 6541233Abstract: A &bgr;-Glucanase enzyme capable of hydrolytically cleaving mixed glucans is presented. The &bgr;-Glucanase is sufficiently stable under alkaline conditions for use in industrial cleaning processes, especially in the brewing industry.Type: GrantFiled: May 1, 2000Date of Patent: April 1, 2003Assignee: Henkel Kommanditgesellschaft auf AktienInventors: Wolfgang Hillen, Karl-Heinz Mauer
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Patent number: 6534101Abstract: The present invention relates to a novel micro-organism, Penicillium funiculosum, to a new enzymes mixture obtained from it, and nucleic acid sequences encoding such enzymes.Type: GrantFiled: April 7, 2000Date of Patent: March 18, 2003Assignee: Aveatis Animal Nutrition S.A.Inventors: Alain Sabatier, Neville Marshall Fish, Nigel Paterson Haigh
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Publication number: 20030044951Abstract: Rural biomass and other cellulosic materials are converted to a protein-enriched animal feed supplement or to single-cell protein by a series of bio-reactions. A first stage bio-reaction is a solid substrate bio-reaction. Enzymes, such as cellulase, produced by the first-stage bio-reaction are added to a second-stage bio-reaction. Raw second-stage bio-reaction feedstock is pretreated to hydrolyze hemicellulose and/or to partially digest starch in the feedstock. In the second-stage bio-reaction, the feedstock is substantially digested and single-cell protein is harvested in an aerobic bio-reaction, while ethanol is produced in an anaerobic reaction. Alternatively, raw biomass or other cellulosic materials can be treated with organic acid (e.g. maleic acid) combined with dry steam to produce a nutritional product that can be directly used as an animal feed supplement.Type: ApplicationFiled: September 3, 2002Publication date: March 6, 2003Inventors: Robert A. Sporleder, James C. Linden, Herbert A. Schroeder, Donald Johnson, Linda L. Henk, Robert P. Tengerdy, George Szakacs
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Publication number: 20030044955Abstract: Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence.Type: ApplicationFiled: March 20, 2002Publication date: March 6, 2003Applicant: Genencor International, Inc.Inventors: Randy Michael Berka, Daniel Cullen, Gregory Lawrence Gray, Kirk James Hayenga, Virgil Bryan Lawlis
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Patent number: 6503540Abstract: The present invention is directed to cloning and characterization of bves (blood vessel/epicardial substance), a cDNA expressed in developing and adult heart and skeletal muscle cells in chick, mouse and human. Also provided are applications of Bves as a marker for cardiovascular or skeletal muscle diseases.Type: GrantFiled: March 24, 2000Date of Patent: January 7, 2003Inventors: David E. Reese, David M. Bader
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Patent number: 6485953Abstract: The present invention relates to a dental plaque hydrolyzing or inhibiting enzyme having simultaneously dextranase and amylase activities.Type: GrantFiled: July 18, 2000Date of Patent: November 26, 2002Assignee: Lifenza Co. Ltd.Inventors: Doman Kim, Do-Won Kim, Soo-Jin Heo, Su-Jin Ryu
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Publication number: 20020155574Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.Type: ApplicationFiled: July 31, 2001Publication date: October 24, 2002Applicant: Novozymes A/SInventors: Thomas Thisted, Soren Kjaerulff, Carsten Andersen, Claus Crone Fuglsang
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Publication number: 20020151073Abstract: The present invention relates to a transcription factor found in filamentous fungi, especially in Aspergillii, DNA sequences coding for said factor, its transformation into and expression in fungal host organisms, and the use of said factor in such hosts for increasing the expression of a polypeptide of interest being produced by said host.Type: ApplicationFiled: August 1, 2001Publication date: October 17, 2002Applicant: Novozymes A/SInventor: Tove Christensen
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Patent number: 6380147Abstract: The invention relates to detergents characterized in that they contain &agr;-amylase from Bacillus amyloliquefaciens and protease from Bacillus lentus, optionally modified by genetic engineering, in addition to our usual ingredients compatible with said enzymes.Type: GrantFiled: February 9, 2001Date of Patent: April 30, 2002Assignee: Henkel Kommanditgesellschaft auf AktienInventors: Horst-Dieter Speckmann, Beatrix Kottwitz, Karl-Heinz Maurer, Christian Nitsch
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Patent number: 6355228Abstract: Disclosed is a polypeptide hybrid containing an amino acid sequence with binding affinity for mutan, the amino acid sequence being bound to an active component useful for oral care purposes; an oral care composition comprising a mutan binding domain; an oral care product comprising such an oral care composition of the invention; and finally the use of a mutan binding polypeptide hybrid or a single unit MBD for oral care purposes, including preventing dental plaque formation and/or removal of existing dental plaque.Type: GrantFiled: April 20, 1999Date of Patent: March 12, 2002Assignee: Novozymes A/SInventor: Claus Crone Fuglsang
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Patent number: 6352851Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.Type: GrantFiled: July 12, 1999Date of Patent: March 5, 2002Assignee: Novozymes A/SInventors: Bjarne Roenfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
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Patent number: 6338861Abstract: A natural flavor or fragrance can be easily and effectively produced by using a “unicellularized plant”, obtained by unicellularizing, without destroying, the cells of a plant usable as a starting material for a natural flavor or fragrance, as a material for the natural flavor or fragrance and further applying an enzymatic treatment or chemical treatment or continuously heating the same, to thereby remarkably shorten the aging period for producing the natural flavor or fragrance, or by homogenizing a plant usable as the starting material quickly after harvesting to thereby promote the aging.Type: GrantFiled: March 26, 1999Date of Patent: January 15, 2002Assignee: Shiseido Company, Ltd.Inventors: Yoko Gozu, Mineyuki Yokoyama, Shoko Yamaguchi, Katsuyuki Yomogida, Shinichiro Haze
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Publication number: 20010034335Abstract: Apoptosis inducers, anticancer drugs and carcinostatic drugs containing sulfated-fucose-containing polysaccharide(s) and/or degradation product(s) thereof, and a method for inducing apoptosis by using sulfated-fucose-containing polysaccharide(s) and/or degradation product(s) thereof as the active ingredient. A degrading enzyme which is useful in the production of the degradation products of sulfated-fucose-containing polysaccharides.Type: ApplicationFiled: January 29, 2001Publication date: October 25, 2001Inventors: Takeshi Sakai, Hideo Kitano, Fu-Gong Yu, Shinji Nakayama, Kaoru Kojima, Hitomi Kimura, Yoshikuni Nakanishi, Kaoru Katayama, Takanari Tominaga, Kazuo Shimanaka, Katsushige Ikai, Ikunoshin Kato
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Patent number: 6297037Abstract: Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.Type: GrantFiled: February 10, 1994Date of Patent: October 2, 2001Inventors: Christopher C. Barnett, Colin Mitchinson, Scott D. Power, Carol A. Requadt
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Patent number: 6197070Abstract: Fabrics are laundered in detergent compositions containing a mixture of &agr;-amylase enzymes to remove malodorous materials.Type: GrantFiled: November 13, 1998Date of Patent: March 6, 2001Assignee: The Procter & Gamble CompanyInventors: Thomas Wilhelm Hörner, Kristien Greta Govers
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Patent number: 6183741Abstract: The present invention is a process for reducing gelatin insolubles which includes providing a gelatin solution and adding to the gelatin solution amylase in an amount to provide a concentration of amylase of at least 0.1 ppm for a time sufficient to reduce gelatin insolubles. The present invention also provides a gelatin having a Bloom strength of from 60 to 400 and a concentration of amylase of greater than 0.1 ppm.Type: GrantFiled: October 30, 1998Date of Patent: February 6, 2001Assignee: Eastman Kodak CompanyInventors: Anne G. Rowlands, Robert F. Rainville
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Patent number: 6165761Abstract: The properties of dough or bread can be improved by the addition of a carbohydrate oxidase which can oxidize the reducing end of an oligosaccharide more efficiently than the corresponding monosaccharide, e.g., preferentially oxidizing maltodextrins or cellodextrins over glucose. A novel carbohydrate oxidase having the capability to oxidize maltodextrins and cellodextrins more efficiently than glucose may be obtained from a strain of Microdochium, particularly M. nivale. The amino acid sequence of the novel carbohydrate oxidase has very low homology (<20% identity) with known amino acid sequences.Type: GrantFiled: December 21, 1998Date of Patent: December 26, 2000Assignee: Novo Nordisk A/SInventors: Palle Schneider, S.o slashed.ren Christensen, Lone Dybdal, Claus Crone Fuglsang, Feng Xu, Elizabeth Golightly
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Patent number: 6100073Abstract: Novel acid-stable and thermo-stable enzymes having .alpha.-1,4 hydrolytic activity and a .alpha.-1,6 hydrolytic activity which are derived from strains of the genus Sulfolobus. These enzymes are capable of expressing high levels of .alpha.-1,4 hydrolytic activity, including the maximum .alpha.-1,4 hydrolytic activity thereof, at highly acidic pHs of between about 2.5 and about 4.5. These .alpha.-amylases are further capable of expressing high levels of .alpha.-1,4 hydrolytic activity, including the maximum .alpha.-1,4 hydrolytic activity thereof, at high temperatures of between about 90.degree. C. and about 120.degree. C. Particularly disclosed herein are such enzymes which are derived from strains of the species S. acidocaldarius and, in particular, Sulfolobus acidocaldarius DSM 639. Modified starch degradation (liquefaction and saccharification) processes using these novel enzymes are also disclosed herein.Type: GrantFiled: September 29, 1997Date of Patent: August 8, 2000Assignee: Genencor International, Inc.Inventors: Philippe Deweer, Antione Amory
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Patent number: 6080568Abstract: Novel .alpha.-amylase enzymes are disclosed in which one or more of residues corresponding to A210, H405 and T412 in Bacillus licheniformis are mutated. The disclosed .alpha.-amylase enzymes show altered or improved stability and/or activity profiles.Type: GrantFiled: August 19, 1997Date of Patent: June 27, 2000Assignee: Genencor International, Inc.Inventors: Anthony G. Day, Barbara A. Swanson
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Patent number: 6022724Abstract: The present invention relates to a method of constructing a variant of a parent Termamyl-like .alpha.-amylase, which variant has .alpha.-amylase activity and at least one altered property as compared to the parent .alpha.-amylase, comprisesi) analyzing the structure of the parent Termamyl-like .alpha.-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like .alpha.-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like .alpha.-amylase (as evaluated on the basis of structural or functional considerations),ii) constructing a Termamyl-like .alpha.-amylase variant, which as compared to the parent Termamyl-like .alpha.-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally,iii) testing the resulting Termamyl-like .alpha.-amylase variant with respect to the property in question.Type: GrantFiled: July 18, 1996Date of Patent: February 8, 2000Assignee: Novo Nordisk A/SInventors: Allan Svendsen, Henrik Bisg.ang.rd-Frantzen, Torben Borchert
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Patent number: 6008026Abstract: Novel .alpha.-amylase enzymes are disclosed in which one or more disulfide bonds are introduced into the enzyme via addition or substitution of a residue with a cysteine. The disclosed .alpha.-amylase enzymes show altered or improved stability and/or activity profiles.Type: GrantFiled: July 11, 1997Date of Patent: December 28, 1999Assignee: Genencor International, Inc.Inventor: Anthony G. Day
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Patent number: 5989169Abstract: The present invention relates to a method of constructing a variant of a parent Termamyl-like .alpha.-amylase, which variant has .alpha.-amylase activity and at least one altered property as compared to the parent .alpha.-amylase, comprising i) analyzing the structure of the parent Termamyl-like .alpha.-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like .alpha.-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like .alpha.-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like .alpha.-amylase variant, which as compared to the parent Termamyl-like .alpha.-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and iii) testing the resulting Termamyl-like .alpha.-amylase variant for the property in question.Type: GrantFiled: February 13, 1996Date of Patent: November 23, 1999Assignee: Novo Nordisk A/SInventors: Allan Svendsen, Henrik Bisg.ang.rd-Frantzen, Torben Vedel Borchert
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Patent number: 5962276Abstract: The present invention describes a simple and inexpensive purification method for acid-stable fungal alpha-amylase wherein the alpha-amylase is obtained without significant loss of enzyme activity. The purified acid-stable alpha-amylase which is obtained is substantially free from glucoamylase. The method for obtaining an acid-stable alpha-amylase comprises the steps ofadjusting the pH of the enzyme containing solution to a value between 1 and 8,heating the solution to a temperature and for a time sufficient to inactivate glucoamylase,removing the denatured glucoamylase.The purified acid-stable alpha-amylase is used for the conversion of starch. The purified alpha-amylase is also used in an immobilized form.Type: GrantFiled: February 17, 1998Date of Patent: October 5, 1999Assignee: Cerestar Hold B.V.Inventors: Ronny Vercauteren, Els Ginelle Alexander Dendooven, An Amanda Jules Heylen
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Patent number: 5958739Abstract: Novel .alpha.-amylase enzymes are disclosed in which one or more asparagine residues are substituted with a different amino acid or deleted. The disclosed .alpha.-amylase enzymes show altered or improved low pH starch hydrolysis performance, stability and activity profiles.Type: GrantFiled: February 20, 1997Date of Patent: September 28, 1999Assignee: Genencor International Inc.Inventors: Colin Mitchinson, Carol Requadt, Traci Ropp, Leif P. Solheim, Christopher Ringer, Anthony Day
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Patent number: 5866526Abstract: An enzyme preparation comprising a modified enzyme selected from the group consisting of an amylase, lipase, oxidorcductase, pectinace or hemicellulase, the modified enzyme having an improved performance due to an alkaline pI and/or increased surface activity obtained by chemical modification or amino acid substitution, is useful e.g., in detergents, in baking flour, in animal feed, in the manufacture of cellulosic fabrics and for the treatment of lignocellulosic fibers.Type: GrantFiled: May 2, 1996Date of Patent: February 2, 1999Assignee: Novo Nordisk A/SInventors: Arne Agerlin Olsen, Allan Svendsen, Kim Borch, Henrik Lund, Marianne Thellersen, Peter Rosholm, Niels Munk
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Patent number: 5830837Abstract: A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.Type: GrantFiled: November 22, 1994Date of Patent: November 3, 1998Assignee: Novo Nordisk A/SInventors: Henrik Bisg.ang.rd-Frantzen, Torben Vedel Borchert, Allan Svendsen, Marianne Thellersen, Pia Van der Zee
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Patent number: 5824532Abstract: Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.Type: GrantFiled: June 6, 1995Date of Patent: October 20, 1998Assignee: Genencor International, Inc.Inventors: Christopher C. Barnett, Colin Mitchinson, Scott D. Power, Carol A. Requadt
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Patent number: 5801043Abstract: A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.Type: GrantFiled: June 2, 1995Date of Patent: September 1, 1998Assignee: Novo Nordisk A/SInventors: Henrik Bisg.ang.rd-Frantzen, Torben Vedel Borchert, Allan Svendsen, Marianne Thellersen, Pia Van der Zee
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Patent number: 5783546Abstract: A detergent composition comprising an amylase enzyme which shows CMCase activity and/or is an amylase showing a positive immunological cross reaction with the antibody of the Fungamyl amylase, or an amylase produced by a host organism in which the gene encoding the Fungamyl has been cloned.Type: GrantFiled: October 18, 1996Date of Patent: July 21, 1998Assignee: Procter & Gamble CompanyInventors: Jean-Luc Philippe Bettiol, Michael Alan John Moss, Christiaan Arthur Jacques Kamiel Thoen, Stanton Lane Boyer, Michael Stanford Showell, Janice Jeffrey
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Patent number: 5763221Abstract: A method for transforming strain PF1022, which produces a cyclic depsipeptide (substance PF1022) and belongs to the order Agonomycetales of the class Hyphomycetes has been established by using a plasmid constructed with a promoter, a terminator, a marker gene and a target gene. When the strain PF1022 was transformed by this method, a gene encoding an enzyme, which is the target gene, was transduced into the host. As a result, the utilization of nutrients by the strain PF1022 was improved and, further, its cyclic depsipeptide productivity was also improved.Type: GrantFiled: February 7, 1997Date of Patent: June 9, 1998Assignee: Meiji Seika Kaisha, Ltd.Inventors: Kaoru Aoyagi, Manabu Watanabe, Kohji Yanai, Takeshi Murakami
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Patent number: 5756714Abstract: According to the invention a method is provided for liquefying starch comprising the steps of treating the starch prior to or simultaneously with liquefying the starch to inactivate and/or remove the enzyme inhibiting composition present in the starch and form treated starch; adding .alpha.-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Effective means to treat the starch include the addition of a phytate degrading enzyme and heat treatment, optionally followed by filtration or centrifugation, of granular starch or a starch solution.Type: GrantFiled: March 27, 1995Date of Patent: May 26, 1998Assignee: Genencor International, Inc.Inventors: Richard L. Antrim, Colin Mitchinson, Leif P. Solheim
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Patent number: 5753460Abstract: A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.Type: GrantFiled: October 10, 1996Date of Patent: May 19, 1998Assignee: Novo Nordisk A/SInventors: Henrik Bisgard-Frantzen, Torben Vedel Borchert, Allan Svendsen, Marianne Thellersen, Pia Van der Zee
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Patent number: 5736499Abstract: Novel .alpha.-amylase enzymes are disclosed in which one or more asparagine residues are substituted with a different amino acid or deleted. The disclosed .alpha.-amylase enzymes show altered or improved low pH starch hydrolysis performance, stability and activity profiles.Type: GrantFiled: June 6, 1995Date of Patent: April 7, 1998Assignee: Genencor International, Inc.Inventors: Colin Mitchinson, Carol Ann Requadt, Traci Helen Ropp, Leif P. Solheim
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Patent number: 5731280Abstract: The present invention relates to lipase and .alpha.-amylase variants, stabilized towards the inactiviation caused by peroxidase systems, in which variants a naturally occurring tryosine residue has been deleted, substituted with a different amino acid residue at one or more positions. The invention also relates to a method of stabilizing a lipase or an .alpha.-amylase towards the inactivation caused by the preoxidase systems, and detergent compositions comprising a lipase and/or .alpha.-amylase variant of the invention.Type: GrantFiled: June 15, 1995Date of Patent: March 24, 1998Assignee: Novo Nordisk A/SInventors: Egon Nielsen, Grethe Rasmussen, Torben Halkier
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Patent number: 5665585Abstract: The invention is directed to amino acid and DNA sequences of a unique glucoamylase P that has a high debranching activity, a Trichoderma host cell, transformed with such sequences, the expression of such recombinant glucoamylase P, and the industrial uses for the recombinant enzyme and hosts transformed therewith.Type: GrantFiled: February 7, 1995Date of Patent: September 9, 1997Assignee: Alko-Yhiot OyInventors: Tuula Torkkeli, Vesa Joutsjoki, Helena Torkkeli, Arja Vainio, Richard Fagerstrom, Sirpa Aho, Matti Korhola, Helena Nevalainen