From Aspergillus Patents (Class 435/225)
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Patent number: 10927358Abstract: The invention is directed to novel variant endoglucanases.Type: GrantFiled: January 15, 2020Date of Patent: February 23, 2021Assignee: Fornia BioSolutions, Inc.Inventors: Jie Yang, Khin Oo, Goutami Banerjee, Xiyun Zhang, Wenhua Lu, Stephen Joshua Macaso Millet, Tatsuya Fukushima, Eric Lin Hu, Imad N. Sawaya, Marielle De Jesus Palatino
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Patent number: 9399769Abstract: The present invention relates to isolated polypeptides having carboxypeptidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: December 12, 2013Date of Patent: July 26, 2016Assignee: Novozymes, Inc.Inventor: Michael Rey
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Patent number: 8815571Abstract: Novel fiber processing methods and the products obtained therefrom are disclosed. Methods may include thermochemical and/or enzymatic hydrolysis of fiber feedstocks including distillers' dried grains, distillers' dried grains with solubles, soyhull, miscanthus and switchgrass. Enzymatic hydrolysis includes hydrolysis with cellulase, hemicellulase, and protease.Type: GrantFiled: October 8, 2008Date of Patent: August 26, 2014Assignee: Archer Daniels Midland Co.Inventors: Charles A. Abbas, Wu-Li Bao
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Publication number: 20140205587Abstract: Gluten-degrading proteases can be used to degrade gluten and for making gluten-containing food safer for patients suffering from gluten intolerance.Type: ApplicationFiled: June 13, 2012Publication date: July 24, 2014Applicant: ALVINE PHARMACEUTICALS, INC.Inventor: Matthew John Siegel
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Patent number: 8765442Abstract: The invention relates to a process for production of an enzyme product having a plurality of enzyme activities obtained by fermentation of an Aspergillus strain.Type: GrantFiled: December 21, 2009Date of Patent: July 1, 2014Assignee: DuPont Nutrition Biosciences ApSInventor: Mette Zacho Grønfeldt
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Patent number: 8741626Abstract: The present invention relates to a mutant strain of Aspergillus sojae with an enhanced protease activity and a preparation method of a natural taste enhancer using the same. More specifically, the present invention relates to the mutant strain of Aspergillus sojae with an enhanced protease activity is obtained by selecting a strain with high protease activity and treating it with N-methyl-N?-Nitroso-N-nitrosoguanidine (NTG) and inducing a mutation through irradiating, and a preparation method of a natural taste enhancer using protein hydrolysate obtained by hydrolyzing the protein sources with their cultures.Type: GrantFiled: October 29, 2009Date of Patent: June 3, 2014Assignee: CJ Cheiljedang CorporationInventors: Hyun Chi, Seong-Jun Cho
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Patent number: 8735563Abstract: The present invention relates to isolated polynucleotides having promoter activity the juse of the isolated polynucleotides for the procuction of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.Type: GrantFiled: June 23, 2011Date of Patent: May 27, 2014Assignee: Novozymes A/SInventors: Hiroshi Teramoto, Hiroaki Udagawa
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Patent number: 8580557Abstract: The present invention relates to a casein hydrolysate containing free amino acids and in vivo indigestible peptides having minimally suppressed in vivo enzymatic digestibility, and expected to express functions, such as hypotensive effect, in living organism, and to a method for preparing such a hydrolysate, and use thereof. The casein hydrolysate of the present invention contains free amino acids and peptides, such as in vivo indigestible peptides including Xaa-Pro and Xaa-Pro-Pro, obtained by hydrolyzing animal milk casein to have an average chain length of not longer than 2.1 in terms of the number of amino acid residues, and has ACE inhibitory activity or hypotensive effect.Type: GrantFiled: September 1, 2010Date of Patent: November 12, 2013Assignee: Calpis Co., Ltd.Inventors: Naoyuki Yamamoto, Seiichi Mizuno, Takanobu Gotou, Keiichi Matsuura, Tadashi Shinoda, Hideo Nishimura
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Publication number: 20130156884Abstract: A food supplement comprises at least one protease enzyme selected from (i) CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus; (ii) CAS #9001-92-7, IUB 3.4.21.7 protease from Bacillus; (iii) CAS #9001-61-0, IUB 3.4.11.1 protease from Aspergillus; (iv) CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus; (v) CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus; (vi) CAS #9025-49-4, IUB 3.4.23.18 protease from Aspergillus; and (vii) combinations thereof. The food supplement may further comprise a protein, a stabilizer, a matrix modifier, a carrier, a preservative, a bulking agent, a dessicant, an emulsifier, an enzyme coating, or combinations thereof. Disclosed is a method of increasing protein absorption in the gastrointestinal system of a human being comprising the step of ingesting the food supplement as identified above.Type: ApplicationFiled: December 19, 2011Publication date: June 20, 2013Applicant: TRIARCO INDUSTRIESInventor: Mark L. Anderson
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Publication number: 20130122545Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: January 17, 2013Publication date: May 16, 2013Applicant: NOVOZYMES A/SInventor: NOVOZYMES A/S
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Publication number: 20130065810Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.Type: ApplicationFiled: April 28, 2010Publication date: March 14, 2013Applicant: AB Enzymes OyInventors: Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo
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Publication number: 20120276075Abstract: The present invention relates to a novel enzyme composition comprising a prolyl protease and tripeptidyl proteases having unique catalytic properties. The present invention further relates to methods for producing the enzyme composition as well as a pharmaceutical composition and a food supplement containing the enzyme composition and its use in the degradation of polypeptides.Type: ApplicationFiled: December 20, 2010Publication date: November 1, 2012Applicant: CENTRE HOSPITALIER UNIVERSITAIRE VAUDOIS (CHUV)Inventors: Michel Monod, Eric Grouzmann
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Patent number: 8198068Abstract: A method of forming localized variation of color density in the surface of a dyed cellulosic fabric with reducing back staining, with a composition comprising a cellulose having the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having at least 75% sequence identity with SEQ ID NO: 2 is provided. A method for biopolishing a cellulose-containing fabric by using the new endoglucanase is also provided.Type: GrantFiled: February 19, 2009Date of Patent: June 12, 2012Assignee: Novozymes A/SInventors: Guifang Wu, Sarah Teter, Paul Harris, Weijian Lai
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Publication number: 20120141628Abstract: The invention relates to a method for the prevention or reduction of haze in a beverage by the addition of an prolyl-specific endoprotease and to new beverages obtainable by the method according to the invention. It also relates to new endoproteases. Sequence information of a genomic DNA, cDNA as well as protein sequences.Type: ApplicationFiled: January 13, 2012Publication date: June 7, 2012Applicant: DSM IP Assets B.V.Inventors: Michel LOPEZ, Luppo Edens
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Patent number: 7998476Abstract: A method for treating various diseases, conditions and injuries with a protease preparation derived from Aspergillus orzyze and made using potato dextrin as the carbohydrate source is described. The method comprises orally administering the Aspergillus oryzae protease preparation on an empty stomach and in an amount greater than about 2,000,000 HUT per day. Additionally, a method for treating various diseases, conditions and injuries with a protease derived from Aspergillus oryzae made using potato dextrin as the carbohydrate source along with a nutritional supplement of vitamins and minerals is also described. The method comprises orally administering the Aspergillus oryzae protease preparation on an empty stomach in an amount of greater than 2,000,000 HUT per day and administering the dietary supplement of vitamins and minerals orally with food.Type: GrantFiled: November 16, 2007Date of Patent: August 16, 2011Assignee: Standard Biologics, Inc.Inventor: Joseph Cerceo
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Patent number: 7897728Abstract: The present invention provides a process to make a gelatine hydrolysate, a gelatine hydrolysate, and gelatine compositions including gelatine hydrolysates. More specifically, the invention provides gelatine compositions having a reduced tendency to cross-link and improved dissolution properties.Type: GrantFiled: May 31, 2006Date of Patent: March 1, 2011Assignee: Gelita AGInventors: John M. Dolphin, Jason D. Russell
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Publication number: 20110028375Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderm. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.Type: ApplicationFiled: April 28, 2010Publication date: February 3, 2011Applicant: AB Enzymes OyInventors: Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo, George Szakacs
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Publication number: 20110003729Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.Type: ApplicationFiled: April 28, 2010Publication date: January 6, 2011Applicant: AB Enzymes OyInventors: Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo
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Patent number: 7785824Abstract: Process for the preparation of a hydrolysed casein product comprising tripeptides VPP, IPP and/or LPP, wherein a substrate comprising casein or casein fragments is subjected to enzyme treatment wherein the enzyme is derived from Aspergillus, wherein the enzyme concentration is 2-10 wt. % based on casein and that the enzyme has a high proteolytic activity.Type: GrantFiled: April 23, 2004Date of Patent: August 31, 2010Assignee: Conopco Inc.Inventors: Monique Cecilia van der Burg-Koorevaar, René´ Bernardus Draaisma, Johannes Schalk
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Patent number: 7608697Abstract: A method of enzymatically producing a protein hydrolysate from a protein substrate is described, wherein a proline-specific endoprotease or a composition containing a proline-specific endoprotease and optionally a subtilisin or a metallo endoprotease, and other enzymes such as carboxypeptidases, is used to produce a protein hydrolysate enriched in peptide fragments having a carboxy terminal proline residue. Such protein hydrolysates may be used as such or to reduce bitterness in foods nutritionally supplemented by protein hydrolysates, as well as to produce hydrolysate containing foodstuffs having low antigenicity.Type: GrantFiled: December 6, 2001Date of Patent: October 27, 2009Assignee: DSM IP Assets B.V.Inventors: Luppo Edens, Robertus Antonius Mijndert Van Der Hoeven, Veronique Delest
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Publication number: 20090181007Abstract: A composition for in vitro use as a culture medium or in vivo use as a pharmaceutical composition or a medical device, capable of accelerating the differentiation of stem cells into cells with a chondrocytic phenotype and of restoring the original trophism of chondrocytes, is described. The composition comprises, in combination, at least one proteolytic enzyme, at least one growth factor and at least one from a sugar, an amino acid, a vitamin factor, a vitamin, a nucleotide and a nucleoside, in a physiologically acceptable carrier or diluent. A method of differentiating stem cells in cells having a chondrocytic phenotype, the cells obtained by the method and their uses, for example in human or animal cell therapy, for example by CBMP (Cellular Based Medicinal products) are also described.Type: ApplicationFiled: April 12, 2007Publication date: July 16, 2009Inventors: Luisa Gennero, Antonio Ponzetto, Enrico De Vivo, Luciano Contu, Emanuella Morra, Chiara Cesano, Giovanni Cavallo, Giuseppe Cicero, Gian Piero Pescarmona, Tetyana Denysenko, Maria Augusta Roos, Maurizio Sara
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Patent number: 7323327Abstract: The invention relates to newly identified gene sequences that encode novel proteases obtainable from Aspergillus niger. The invention features the full length gene sequence of the novel genes, their cDNA sequences as well as the full-length functional protein and fragments thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with DNA according to the invention and cells wherein a protease according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.Type: GrantFiled: February 22, 2002Date of Patent: January 29, 2008Assignee: DSM IP Assets B.V.Inventors: Luppo Edens, Albertus Van Dijk, Philipp Krubasik, Kaj Albermann, Alexander Stock, Erik Kimpel, Sabine Klugbauer, Christian Wagner, Andreas Fritz, Wilk Von Gustedt, Oliver Heinrich, Dieter Maier, Fabio Spreafico, Ulrike Folkers, Sylvia Hopper, Wolfram Kemmner, Pamela Tan, Josephine Stiebler, Richard Albang
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Patent number: 7297512Abstract: From fish egg skin produced by utilization of roe grains of various fish, constituent proteins thereof are enzymatically degraded to obtain peptides and amino acids, of which effective utilization is achieved. The invention provides a method for producing amino acids and peptides (useful as nutrient enhancers for foods) from fish egg skins which comprises treating cold water-washed fish egg skins with ozonized water at room temperature or below, subsequently, degrading the resultant product with a proteolytic enzyme produced by Bacillus subtilis, or further treating with a proteolytic enzyme produced by Aspergillus oryzae, to degrade myogenic fiber proteins (contractile proteins: myosins) in the fish egg skin, and then concentrating/drying the degraded solution.Type: GrantFiled: December 12, 2003Date of Patent: November 20, 2007Assignee: Fuji Bio Technology Institute Co., Ltd.Inventor: Kunihiko Kodaka
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Patent number: 7255888Abstract: The present invention discloses a method for producing foods and/or beverage having improved taste and/or flavour, comprising reacting a microbial aminopeptidase on a protein material optionally under the co-existence of a protease, wherein said aminopeptidase has the properties of: (a) having an activity of catalyzing the reaction of specifically releasing a glutamic acid and an aspartic acid from the N-terminal of a peptide and/or a protein; (b) having 50% or more activity at pH6.0-9.0 as compared with the activity at the optimum pH; (c) having 40% or more activity after heating at 25-60° C., pH7.5 for 30 minutes as compared with the activity of the non-heated enzyme; (d) having a molecular weight of about 40-60 kD as measured by SDS-PAGE and about 300-480 kD as measured by native-PAGE; (e) having a hydrolyzing activity of the aminopeptidase toward Glu-Glu dipeptide is 5 U/mg or more, preferably 10 U/mg or more.Type: GrantFiled: November 28, 2005Date of Patent: August 14, 2007Assignee: Ajinomoto Co., Inc.Inventors: Tomohiro Sakamoto, Nami Nakamura, Tomohiro Kodera, Noriki Nio, Noriaki Yamada, Hidehiko Wakabayashi
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Patent number: 7087422Abstract: The object of the present invention is to provide Koji mold aminopeptidases capable of efficiently hydrolyzing persistent peptides and also genes encoding the aminopeptidases. The present invention provides Aspergillus nidulans aminopeptidase and nucleic acid molecules encoding it. In particular, the present invention provides a protein having an amino acid sequence represented by amino acid Nos. 1 to 519 in SEQ ID NO: 2, or a protein containing the substitution, deletion, insertion, addition or inversion of one or more amino acids in said sequence, and which protein has an activity of catalyzing the reaction for releasing an amino acid at an N-terminal of a peptide, and nucleic acid molecules encoding them.Type: GrantFiled: September 22, 2003Date of Patent: August 8, 2006Assignee: Ajinomoto Co., Inc.Inventors: Kyoko Koibuchi, Daiki Ninomiya, Mari Kojima, Yoichi Ueda, Jun-ichi Maruyama, Katsuhiko Kitamoto
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Patent number: 6982163Abstract: A process for preparing beta-fructofuranosidase enzyme and a process for producing fructooligosaccharides, in which the preparation of the enzyme is obtained by cultivating the fungus Aspergillus niger, either wild or mutated, in a preferably semi-solid culture medium, in order to produce an extracellular enzyme, which is submitted to transfructosylation for producing fructooligosaccharides comprising sugars which are formed by one unit of sacrose and by two, three and four units of fructose.Type: GrantFiled: September 30, 2002Date of Patent: January 3, 2006Assignee: Usina Da Barra S/A-Acucar e AlcoolInventors: Yong Kun Park, Glaucia Maria Pastores
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Patent number: 6951750Abstract: Provided are a protein comprising the amino acid sequence as shown in SEQ ID NO: 2 and having prolidase activities, a prolidase gene coding for the protein, a prolidase gene comprising the nucleotide sequence as shown in SEQ ID NO: 1, recombinant DNA having the gene inserted into vector DNA, and a transformant or transductant comprising the recombinant DNA, and a method for producing prolidase using the transformant or transductant. The present invention can improve the prolidase through protein engineering. The present invention can be also used for improving microorganisms used in the production of enzymes for food processing and fermented foods.Type: GrantFiled: December 23, 2002Date of Patent: October 4, 2005Assignees: Kikkoman Corporation, Noda Institute for Scientific ResearchInventors: Kotaro Ito, Takeharu Nakahara, Yasuji Koyama, Toshifumi Matsuda, Tadashi Takahashi, Kenichiro Matsushima, Genryou Umitsuki, Tsutomu Masuda
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Patent number: 6899876Abstract: Methods and compositions that can reduce the symptoms of autism in a human patient comprising administering a physiologically effective amount of one or both of a purified casomorphin inhibitor selected from the group consisting of a casomorphinase and a casomorphin ligand, and a physiologically effective amount of a purified gluteomorphin inhibitor selected from the group consisting of a gluteomorphinase and a gluteomorphin ligand, to a human patient in sufficient quantities to reduce the effects of the autism. In some embodiments, the compositions and methods further comprise a physiologically effective amount of an enkephalin inhibitor, preferably an enkephalinase, and a physiologically effective amount of an endorphin inhibitor, preferably an endorphinase.Type: GrantFiled: December 6, 2002Date of Patent: May 31, 2005Assignee: ProThera, Inc.Inventor: Devin B. Houston
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Patent number: 6897049Abstract: The present invention relates to a novel methods and compositions for producing hyper and hypo allergenic compositions. Specifically, the present invention comprises neutralizing or reducing the ability of T-cells to recognize epitopes and thus prevent sensitization of an individual to the protein. Alternatively, T-cell epitopes are mutated to produce increased immunogenic reactions.Type: GrantFiled: January 23, 2001Date of Patent: May 24, 2005Assignee: Genencor International, Inc.Inventors: David A. Estell, Fiona A. Harding
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Patent number: 6863918Abstract: An improved infant formula resulting in reduced constipation, abdominal discomfort and gastrointestinal problems, comprises at least one protein component having a phosphorus content of less than 0.75 g P/100 g protein, and at least one lipid component that can be easily digested by an infant. Preferably, it further comprises at least one prebiotic component, and at least one viscosity-improving component. The protein fraction of the formula is preferably a hydrolysate prepared by hydrolysing a protein starting material, especially a whey protein with a combination of at least one endo- and at least one exoproteinase.Type: GrantFiled: December 13, 2000Date of Patent: March 8, 2005Assignee: N.V. NutriciaInventors: Jacob Geert Bindels, Antonie Van Baalen, Robert Johan Joseph Hageman, Peti Huybers, Liliane Marie-Rose Louisa Dominique Dumon
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Patent number: 6846656Abstract: The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.Type: GrantFiled: August 25, 2000Date of Patent: January 25, 2005Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Satoshi Koizumi, Kazuhiko Tabata, Tetsuo Endo, Akio Ozaki
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Patent number: 6821514Abstract: Methods and compositions that can reduce the symptoms of autism in a human patient comprising administering a physiologically effective amount of one or both of a purified casomorphin inhibitor selected from the group consisting of a casomorphinase and a casomorphin ligand, and a physiologically effective amount of a purified gluteomorphin inhibitor selected from the group consisting of a gluteomorphinase and a gluteomorphin ligand, to a human patient in sufficient quantities to reduce the effects of the autism. In some embodiments, the compositions and methods further comprise a physiologically effective amount of an enkephalin inhibitor, preferably an enkephalinase, and a physiologically effective amount of an endorphin inhibitor, preferably an endorphinase.Type: GrantFiled: December 6, 2002Date of Patent: November 23, 2004Assignee: ProThera, Inc.Inventor: Devin B. Houston
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Patent number: 6808708Abstract: Methods and compositions that can reduce the symptoms of autism in a human patient comprising administering a physiologically effective amount of one or both of a purified casomorphin inhibitor selected from the group consisting of a casomorphinase and a casomorphin ligand, and a physiologically effective amount of a purified gluteomorphin inhibitor selected from the group consisting of a gluteomorphinase and a gluteomorphin ligand, to a human patient in sufficient quantities to reduce the effects of the autism. In some embodiments, the compositions and methods further comprise a physiologically effective amount of an enkephalin inhibitor, preferably an enkephalinase, and a physiologically effective amount of an endorphin inhibitor, preferably an endorphinase.Type: GrantFiled: September 5, 2002Date of Patent: October 26, 2004Assignee: ProThera, Inc.Inventor: Devin B. Houston
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Patent number: 6770469Abstract: A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.Type: GrantFiled: February 27, 2001Date of Patent: August 3, 2004Assignee: Amano Pharmaceutical Co., Ltd.Inventors: Shotaro Yamaguchi, Akira Matsuura
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Patent number: 6447772Abstract: Methods and compositions that can reduce the symptoms of autism in a human patient comprising administering a physiologically effective amount of one or both of a purified casomorphin inhibitor selected from the group consisting of a casomorphinase and a casomorphin ligand, and a physiologically effective amount of a purified gluteomorphin inhibitor selected from the group consisting of a gluteomorphinase and a gluteomorphin ligand, to a human patient in sufficient quantities to reduce the effects of the autism. In some embodiments, the compositions and methods further comprise a physiologically effective amount of an enkephalin inhibitor, preferably an enkephalinase, and a physiologically effective amount of an endorphin inhibitor, preferably an endorphinase.Type: GrantFiled: October 1, 1999Date of Patent: September 10, 2002Assignee: Klaire Laboratories, Inc.Inventor: Devin B. Houston
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Patent number: 6379928Abstract: Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence.Type: GrantFiled: December 10, 1999Date of Patent: April 30, 2002Assignee: Genencor International, Inc.Inventors: Randy Michael Berka, Daniel Cullen, Gregory Lawrence Gray, Kirk James Hayenga, Virgil Bryan Lawlis
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Patent number: 6271013Abstract: Aminopeptidases originating from Aspergilli niger are disclosed, which can be produced in a fermentation broth or a liquid concentrate thereof substantially free of endoprotease. Such aminopeptidases may be advantageously employed in the food industry, e.g. in the preparation of bread doughs or cheese.Type: GrantFiled: June 22, 1999Date of Patent: August 7, 2001Assignee: DSM Patents and TrademarksInventors: Laurent Chevalet, Jerome Souppe, Joel De Leseleuc, Jacky Brunet, Martinus J. M. Warmerdam
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Patent number: 6251391Abstract: Compositions and methods are provided to reduce opioid-related symptoms in a human patient of an exorphin selected from the group consisting of a gluteomorphin and a caseomorphin, comprising a physiologically effective amount of a purified casomorphin inhibitor selected from the group consisting of a casomorphinase and a casomorphin ligand, a physiologically effective amount of a purified gluteomorphin inhibitor selected from the group consisting of a gluteomorphinase and a gluteomorphin ligand, and at least one of the group consisting of a physiologically acceptable carrier, adjuvant, excipient, buffer and diluent. In some embodiments, the compositions and methods further comprise a physiologically effective amount of an enkephalin inhibitor, preferably an enkephalinase, and a physiologically effective amount of an endorphin inhibitor, preferably an endorphinase. Preferrably the caseomorphinase is dipeptidyl peptidase IV and the gluteomorphinase is tyrosinase or phenylalaninase.Type: GrantFiled: October 1, 1999Date of Patent: June 26, 2001Assignee: Klaire Laboratories, Inc.Inventors: Randall Eugene Wilkinson, Devin B. Houston
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Patent number: 6171817Abstract: Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence.Type: GrantFiled: November 24, 1997Date of Patent: January 9, 2001Assignee: Genencor International, Inc.Inventors: Randy Michael Berka, Daniel Cullen, Gregory Lawrence Gray, Kirk James Hayenga, Virgil Bryan Lawlis
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Patent number: 6130064Abstract: The invention provides a human SMN-like protein (HSLP) and polynucleotides which identify and encode HSLP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for treating or preventing disorders associated with expression of HSLP.Type: GrantFiled: February 24, 1998Date of Patent: October 10, 2000Assignee: Incyte Pharmaceuticals, Inc.Inventors: Y. Tom Tang, Neil C. Corley, Karl J. Guegler
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Patent number: 6121025Abstract: The present invention relates to a process for producing optically active 3-quinuclidinol or derivatives, wherein a racemic 3-quinuclidinol ester represented by the general formula (I): ##STR1## wherein R represents a straight-chain or branched alkyl group, and (H.sup.+) represents that said ester may be in the form of a salt formed with a mineral acid or an organic acid, is reacted with a microorganism belonging to the genus Aspergillus, Rhizopus, Candida or Pseudomonas having the ability to asymmetrically hydrolyze said ester linkage, a culture of said microorganism, a treated material from said microorganism, an enzyme produced by said microorganism, or an enzyme derived from swine or cattle.According to the present invention, there is provided a process for easily producing optically active 3-quinuclidinol derivatives which are important synthetic intermediates for pharmaceutical preparations etc.Type: GrantFiled: May 5, 1999Date of Patent: September 19, 2000Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Eiji Sato, Kanehiko Enomoto
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Patent number: 6033867Abstract: The present invention relates to a fructosyl amino acid oxidase that is obtainable from a culture of a strain of the genus Aspergillus. This strain grows in a selective medium containing at least either of fructosyl lysine and fructosyl-N.sup..alpha. -Z-lysine and is capable of producing a fructosyl amino acid oxidase.Type: GrantFiled: July 29, 1998Date of Patent: March 7, 2000Assignee: Kyoto Daiichi Kagaku Co., Ltd.Inventors: Nobuo Kato, Yasuyoshi Sakai, Yoshiki Tani, Hiroshi Fukuya, Masayuki Yagi, Toshikatsu Sakai, Kaori Ishimaru, Satoshi Cyousa
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Patent number: 6013452Abstract: The present invention relates to fungi, which do not produce proteases. The fungi of the invention are useful as hosts for the production of proteins susceptible of proteolytic degradation by the proteases usually produced, and the invention consequently encompasses processes for the production of proteins of interest in high yields by using the fungi of the invention. The invention also comprises methods for producing such fungi and DNA constructs to be used in these methods.Type: GrantFiled: May 14, 1998Date of Patent: January 11, 2000Assignee: Novo Nordisk A/SInventors: Tove Christensen, Jan Lehmbeck
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Patent number: 5998190Abstract: An isolated and purified enzyme exhibiting protease activity at a pH of 4-7 which exhibits protease activity in 5% hydrogen peroxide and which is encoded by a DNA sequence which hybridizes to a DNA sequence depicted in SEQ ID NO. 1 or 2.Type: GrantFiled: November 12, 1998Date of Patent: December 7, 1999Assignee: Novo Nordisk A/SInventors: Henrik Dalb.o slashed.ge, Stephan Christgau, Lene Nonboe Andersen, Lene Venke Kofod, Markus Sakari Kauppinen, Jack Bech Nielsen, Claus Dambmann
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Patent number: 5989889Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having tripeptide aminopeptidase activity. The invention also relates; to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.Type: GrantFiled: March 19, 1997Date of Patent: November 23, 1999Assignee: Novo Nordisk Biotech, Inc.Inventors: Michael Rey, Elizabeth Golightly
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Patent number: 5939305Abstract: The present invention relates to a gene encoding an ascomycete or deuteromycete carboxypeptidase Y gene, and host cells modified so as to produce reduced amounts of carboxypeptidase.Type: GrantFiled: November 10, 1997Date of Patent: August 17, 1999Assignee: Novo Nordisk Biotech IncInventors: Debbie Sue Yaver, Sheryl Ann Thompson
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Patent number: 5914259Abstract: Aminopeptidases originating from Aspergilli niger are disclosed, which can be produced in a fermentation broth or a liquid concentrate thereof substantially free of endoprotease. Such aminopeptidases may be advantageously employed in the food industry, e.g. in the preparation of bread doughs or cheese.Type: GrantFiled: January 30, 1997Date of Patent: June 22, 1999Assignee: Gist-brocades B.V.Inventors: Laurent Chevalet, Jerome Souppe, Joel De Leseleuc, Jacky Brunet, Martinus J.M. Warmerdam
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Patent number: 5891669Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) introducing into a respiratory-defective mutant of a cell (i) one or more first nucleic acid sequences which complement the respiratory defect and (ii) a second nucleic acid sequence which encodes the polypeptide; (b) cultivating the cell containing the first and second nucleic acid sequences in a culture medium under aerobic conditions suitable for expression of the first and second nucleic acid sequences; and (c) isolating the polypeptide from the cultivation medium of the cell. The present invention also relates to methods for disrupting a gene in a respiratory-deficient mutant cell. The present invention further relates to respiratory-deficient mutant cells and methods for obtaining such mutant cells.Type: GrantFiled: March 17, 1997Date of Patent: April 6, 1999Assignees: Novo Nordisk A/S, Novoalle,, Novo Nordisk BiotechInventors: Ejner Bech Jensen, Joel R. Cherry, Susan L. Elrod
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Patent number: 5854050Abstract: A DNA construct comprising a DNA sequence encoding an enzyme exhibiting protease activity, which DNA sequence comprises the DNA sequence shown in SEQ ID No. 1 or 2 or an analog of any of these sequences being at least 80% homologous to the DNA sequence shown in SEQ ID No. 1 or 2. The proteases encoded by the DNA sequences have an acid pH optimum.Type: GrantFiled: February 1, 1996Date of Patent: December 29, 1998Assignee: Novo Nordisk A/SInventors: Henrik Dalb.o slashed.ge, Stephan Christgau, Lene Nonboe Andersen, Lene Venke Kofod, Markus Sakari Kauppinen, Jack Bech Nielsen, Claus Dambmann
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Patent number: 5846802Abstract: The present invention concerns a novel DNA sequence coding for an Aspergillus serine protease of the subtilisin-type, an Aspergillus serine protease of the subtilisin-type per se and a method for the preparation thereof. The invention further concerns a novel Aspergillus mutant strain defective in a serine protease of the subtilisin-type, which is useful for the expression of heterologous protein, and a method for the preparation of such a mutant strain.Type: GrantFiled: April 8, 1994Date of Patent: December 8, 1998Inventors: Frank Buxton, Albert Hinnen, Jacob Visser