Abstract: Provided is a method of spotting a probe densely and efficiently on a surface of a solid support. A liquid containing a probe is attached to a solid support as droplets to form spots containing the probe on the solid support by an ink jet method.

Abstract: A test strip for immunochromatography comprising a sample receiving member, a label holding member, and a chromatography membrane is described. The chromatography membrane has a first detection zone and a second detection zone. The second detection zone is formed by utilizing a blocking material and the second substance. A method for producing above the test strip is also described.

Abstract: The subject invention is an immunoassay for the semi-quantitative test kit for determination of human chrionic gonadtropin (hCG) in fluid sample (such as urine) as an aid in the diagnosis of a certain stage of pregnancy. The test device includes five strips having each having a dipping end or sample ends where sample can be applied. Results are indicated by coloration of two bands across a clear area of the strips, one band being coated with a reagent such as hCG antigens and the other with a reagent such as goat/rabbit polyclonal antibody gold conjugate. The combination of color indications on the bands provides the test results.

Abstract: This invention provides an immunochromatography detection apparatus exhibiting excellent convenience, sensitivity, and specificity, a detection method therefore, a kit using the same, and a method for producing such apparatus and kit, by constructing a solid-phase support, on which the conditions for the reaction wherein an analyte specifically binds to a labeled reagent containing a ligand that specifically binds to the analyte and those for the reaction wherein a capture reagent specifically binds to a complex of the analyte and the labeled reagent in the immunochromatography detection method are continuously optimized, and the pretreated specimens are optimized for such reactions.

Abstract: A reagent container having an inner surface upon which at least two reagents are dried, with the first reagent dried on a first area separate from a second area where the second reagent is dried. The first and second reagents are a nucleic acid polymerase and its substrate. A method is disclosed which includes dispensing at least the first and second reagents onto separate areas of the inner surface of the reagent container, and removing excess water from the reagents. The reagent container can be used in a polymerase chain reaction (PCR) assay, an assay that utilizes a reverse transcriptase, a reverse transcriptase polymerase chain reaction, an immuno-PCR assay, a nucleic acid sequence based assay, a proximity ligation assay, a ligase chain reaction assay, a rolling circle amplification assay, and a strand displacement amplification assay.

Abstract: An analytical strip and a detecting method using the analytical strip are provided. The analytical strip includes a substrate having a channel thereon. The channel has a first region, a second region and a third region, which are connected sequentially. A first antibody is localized in the first region. A saccharide and a peroxidase are localized in the first or second region. A second antibody for recognizing a different epitope of an identical antigen with the first antibody is immobilized in the second region. An optical substrate and a substrate reagent including a saccharide oxidase are localized in the third region.

Abstract: A sensor probe suited for implanting into the skin of a person includes a sensor body which may be formed from a polymer which includes 2-hydroxyethyl methacrylate (HEMA). A sensing system is supported by the body. The sensing system exhibits a detectable change when the probe is exposed to the analyte in the fluid. The sensing system may include an enzyme capable of catalyzing a reaction of the analyte to form a reaction product and a dye system which absorbs in the infrared region of the spectrum in response to the reaction product.

Abstract: In the present invention, it is demonstrated for the first time, the influence of electrical current on the ability of surface plasmons to amplify fluorescence signatures. An applied direct current across silver island films (SiFs) of low electrical resistance perturbs the fluorescence enhancement of close-proximity fluorophores. For a given applied current, surface plasmons in “just-continuous” low resistance films are sparsely available for fluorophore dipole coupling and hence the enhanced fluorescence is gated as a function of the applied current.

Abstract: The problem to be resolved is to provide an electron mediator and a fusion body with high affinity with an enzyme, a measuring method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor. The present invention relates to an electron mediator for glucose oxidoreductase comprising extracellular secretion type cytochrome, a fusion body in which the electron mediator is fused with glucose oxidoreductase, a composition for glucose measurement including the electron mediator or fusion body, a gene encoding a new extracellular secretion type cytochrome, and a measurement method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.

Abstract: The present invention relates to a process for detecting one or more analytes in one or more samples of biological origin having complex composition. The present invention also relates to a microarray for quantitative determination of one or more analytes in samples of biological origin having complex composition which are immobilized in measurement ranges of microarray, and also to a quantitative detection method based thereon.

Abstract: The present invention relates to methods and devices for adhering cells in a specific and predetermined position with an adhesive control of internal cell organization, methods for preparing such devices, methods for studying modifications of cell shape and global internal cell organization such as the distribution of cellular compartments, centrosome centering, spindle orientation, internal compartmentalization and internal transports, methods for screening compounds of interest which enhance or inhibit specific cell functions.

Abstract: A device for detecting or quantifying influenza viruses in a sample, which comprises a detection region having a human anti-influenza virus nucleoprotein antibody immobilized onto a support, a sample supply region, and a sample-migrating region; and a kit for detecting or quantifying influenza viruses, which comprises a solid phase in which a human anti-influenza virus nucleoprotein antibody is fixed onto a carrier.

Abstract: A detection device comprises a basal layer fixed at the bottom of a container therein, and a cholinesterase-containing reaction layer is fixed on the basal layer. A sample is added to the detection device, and the presence of a cholinesterase-inhibiting substance in the sample is visually determined through coloring reaction. For detecting an organophosphate agrichemical, the sample is brought into contact with an oxidizing agent on a column to convert it into the oxon form thereof.

Abstract: The present invention provides an immunological detection method that can detect milk allergens, allergens of albumen, flour, buckwheat and peanut with high sensitivity in foods containing these allergens regardless they are denatured/native, and a detection kit to be used therefor. It is a method for detecting allergens by using 2 or more monoclonal antibodies recognizing native and denatured milk allergens, native and denatured albumen allergens, native and denatured flour allergens, native and denatured buckwheat allergens, and native and denatured peanut allergens, using asl casein which is the main protein of milk casein, ?-lactoglobulin which is the main protein of whey, ovalubumin and ovomucoid which are main proteins of albumen, gliadin which is the main protein of flour, protein with a molecular weight of 24 kDa and 76 kDa which are main proteins of buckwheat, and Ara h1 which is the main protein of peanut as an index.

Abstract: Disclosed herein is a biosensor, in which sensing electrodes are formed on an upper insulating substrate and a lower insulating substrate such that, using the sensing electrodes, a measuring device can determine whether a sample (e.g., blood) injected through a sample injection port is completely filled in a sample path and determine a time point at which the sample is injected and a time point at which the sample is completely filled. Therefore, it is possible to estimate the flow characteristics of the sample and the flow rate and use the estimated values to correct the measurement result, thus obtaining a more accurate measurement result.

Abstract: A diagnostic test kit that employs a lateral flow assay device and a plurality of assay reagents for detecting a test analyte within a test sample is disclosed. The assay reagents include detection probes that are capable of producing a detection signal representing the presence or quantity of the test analyte in the test sample. To further enhance detection accuracy, calibration probes are also used that are capable of producing a calibration signal representing the presence or quantity of a calibration analyte. The calibration signal may be utilized to calibrate the detection signal.

Abstract: Provided are a method for detecting biomaterials, a method for fabricating a chip for biomaterial detection and a chip for biomaterial detection. The method for detecting biomaterials is characterized by comprising the steps of: (S1) immobilizing polydiacetylene liposomes onto a substrate; (S2) linking the polydiacetylene liposomes together and layering them on the substrate; (S3) immobilizing a material which forms a complementary binding with a subject biomaterial to be detected onto the polydiacetylene liposomes; (S4) exposing the resulted polydiacetylene liposome to UV light so as to form a chip for biomaterial detection; (S5) applying the subject biomaterial to be detected to the chip for biomaterial detection for reaction; and (S6) measuring a fluorescent signal from the chip for biomaterial detection.

Abstract: The current invention is directed towards a device that useful for studying cell migration. It is preferred that the device is configured to study stem cell, particularly hematopoetic stem/precursor cell homing. The device allows for detailed analysis of the various phases of the multiphase homing process. There are also provided herein, novel methods of using the device, including, but not limited to, dissection of the homing process under microenvironmental conditions mimicking healthy conditions, diseased conditions, inflammatory conditions, drug treatment conditions and experimental conditions.

Abstract: Diagnostic in-vitro devices for use in the assaying of biological fluids are provided which include cover plates or backing strips which exhibit hydrophilic properties to assist in transport of the biological fluid or retention of same within the device. Exemplary diagnostic devices include lateral flow devices, microfluidic devices and microtiter plates. The devices may also be comprised of low fluorescent material in order to facilitate any diagnostic determination by use of fluorescent emissions. Hydrophilic properties may be imparted to the cover plates or backing strips by physical or chemical treatment thereof. The cover plates or backing strips may exhibit heat sealable or pressure sensitive properties.

Abstract: Adhesion of platelets to blood vessel walls is the first step that promotes arrest of bleeding by interaction of the platelet receptors with various extracellular matrix proteins that become exposed on vascular injury. A flow chamber is provided for use in analyzing or studying platelet function, in whole blood, either as part of a batch process or in real time. In the flow chambers, an inert polydimethylsiloxane (PDMS) surface is plasma-activated and a homobifunctional cross-linker is used to immobilize platelet-binding proteins onto a chamber wall surface. Immobilized collagen and fibrinogen may thus be assayed by continuously monitoring the adhesion of ADP and Ca2+ activated platelets from a subject, such as a patient having normal or type 2 diabetes (T2D). The flow chamber provides a simplified and robust method for the construction flow chambers which enable the kinetic monitoring of platelet adhesion in whole blood.

Abstract: An assay device comprising a compartment adapted to receive a sealed removable cartridge, wherein said cartridge is adapted to contain a biologic sample and internally comprises two or more assay locations, wherein said cartridge is adapted to facilitate two or more assays of said biologic sample; and an actuator adapted to interface with said cartridge to transport said biologic sample towards at least one of said assay locations.

Abstract: Disclosed is a cell culture article including: a substrate; a tie-layer attached to at least the substrate; and a bio-compatible layer attached to at least the tie layer, the bio-compatible layer having been obtained from surface oxidation of a polymer layer. Also disclosed are methods for making the cell culture article and methods for performing an assay of a ligand with the article.

Abstract: According to the biosensor and the blood component analytical method of the present invention, in a biosensor that is made of a single layer or plural layers of a porous material as shown in FIG. 1, having a reagent holding part and utilizing chromatography, a cell shrinkage reagent is carried on at least part of the reagent holding part, or at least part of a chromatographically developed part that is upstream of the reagent holding part. According to the biosensor having the above-mentioned structure and the blood component analytical method, even when whole blood is a sample, a high-accuracy blood component analysis cart be performed easily and quickly with less cost.

Abstract: Disclosed are a blood tester for measurement of temperature of a sample placed in a blood-testing disc using a thermochromic pigment, and a control method thereof. The disclosed blood-testing disc, the blood tester having the blood-testing disc loaded therein, and the control method of the same, are used to measure a light absorbance of the thermochromic pigment in order to determine a temperature of a sample when a biological material such as blood, blood serum, blood plasma, or sputum, is subjected to analysis, so that the test may be executed at a constant temperature. Accordingly, more reproducible and accurate results are obtainable, and temperature measurement of a sample may be simply and effectively attained by an optical analyzer built in the blood tester, without an alternative instrument or sensor for measurement thereof.

Abstract: The present disclosure relates to methods for attaching bioactive agents to paper products by contacting the paper with a solution comprising colloidal support particles where said colloidal support particles are associated with bioactive agents. In specific embodiment of the disclosure, the colloidal support particles are functionalized poly(N-isopropylacrylamide) microgels. The disclosure further covers the bioactive paper produced by this method as well as uses thereof, in particular for pathogen detection.

Abstract: Methods are provided to process a biological growth plate using a biological growth plate scanner. The scanner includes an imaging device, a processor, and an image processing profile memory. The scanner scans a plate type indicator associated with the biological growth plate. In some embodiments, the processor uses the plate type indicator to verify the suitability of the biological growth plate for use in the scanner. In some embodiments, the processor uses the plate type indicator to unlock the scanner for operation.

Abstract: The process, system and device being claimed are based on the measurement of the changes in impedance produced in the interface of an electrode whereonto bacteria from a host strain have been previously adhered for detecting the desired bacteriophage. The changes produced in said electrode-bacteria interface originate in the phagic action of the bacteriophages on the bacteria adhered onto the surface of the work electrode of a microelectronic sensor device.

Abstract: The invention relates to a method for detecting endotoxins in a sample.

Abstract: A method for detecting an objective substance in a sample using a chromatographic test device having a detection region on which a capture substance capable of binding to the objective substance is immobilized, comprising: mixing the sample and an enzyme-labeled substance capable of binding to the objective substance; trapping the objective substance binding to the enzyme-labeled substance by the capture substance in the detection region, by adding the liquid mixture to the chromatographic test device; adding an anionic surfactant-containing reagent to the detection region in which the objective substance is captured; reacting a luminescent substrate with the enzyme of the enzyme-labeled substance binding to the capture substance through the objective substance in the detection region; and detecting the objective substance in the sample by detecting luminescence generated from the detection region by the reaction of the luminescent substrate with the enzyme. A kit is also disclosed.

Abstract: Polymeric indicators are provided for visually monitoring, detecting, and/or determining the presence of metabolic byproducts from harmful or potentially harmful microorganisms.

Abstract: A method of attaching a cell or a membrane-coated particle-of-interest to a microtube is provided. The method comprising: co-electrospinning two polymeric solutions through co-axial capillaries, wherein a first polymeric solution of the two polymeric solutions is for forming a shell of the microtube and a second polymeric solution of the two polymeric solutions is for forming a coat over an internal surface of the shell, the first polymeric solution is selected solidifying faster than the second polymeric solution and a solvent of the second polymeric solution is selected incapable of dissolving the first polymeric solution and wherein the second polymeric solution comprises the cell or the membrane-coated particle-of-interest, thereby attaching the cell or the membrane-coated panicle-of-interest to the microtube. Also provided are microtubes with attached, entrapped or encapsulated cells or membrane-coated particles and methods of using same.

Abstract: A novel dry stick device construction for the determination of an analyte in a sample is provided. The device comprises: (i) optionally a solid support, (ii) at least one reagent pad comprising a reagent or a combination of reagents capable of reacting with the analyte, a derivative of said analyte or an indicator compound for said analyte to provide a detectable signal when in a moistened state, the at least one reagent pad providing a first environment for said reagent(s), said first environment permitting an improved storage stability of the reagent(s) and dry stick device when in a non-moistened state, and (iii) a regulating pad being in contact with the at least one reagent pad, the regulating pad creating a second environment for said reagent(s), when in a moistened state, said second environment permitting an increased rate of reaction between the analyte and the reagent.

Abstract: An object of the present invention is to provide a cell culture vessel which is simple in structure and easy to handle, and is capable of preventing damage to the cells when separated, promoting transport of nutrients and excretion of effete matter, and elevating the culturing efficiency improving effect by the structural features. In order to attain the above object, there is provided a cell culture vessel including a culture section provided with a plurality of projections having an equivalent diameter smaller than the cells to be cultured and the culture section side walls enclosing the culture section, wherein the distance between an arbitrary position on the culture section/side wall boundary line and the nearest projection is smaller than the diameter of the cells to be cultured. The effect of the projections in the vessel given to the cultured cells is enhanced.

Abstract: A method and apparatus are provided for processing a nucleic acid. The apparatus includes a disposable self-contained processing module that contains the nucleic acid and substantially all of the fluids to effect a nanoparticle hybridization test, a pump coupled to the processing module, a valving system disposed between the pump and processing module and a control system coupled to the pump and valving system causing the processing fluids to interact with the nucleic acid to effect a sandwich hybridization test using nanoparticles.

Abstract: It is an object of the present invention to provide a method for simply mixing two or more types of liquids in a porous carrier.

Abstract: Skin cholesterol is measured by applying an adhesive tape onto a selected area of the skin to adhere the tape to the selected skin area and stripping the tape off the selected skin area to obtain a sample representative of the outer stratum corneum layer of the skin, the sample adhering to the tape so as to have exposed skin constituents. The sample is assayed using a detector reagent that specifically binds to cholesterol and in addition has an indicator component that allows quantitation of the amount of cholesterol present in the exposed skin constituents.

Abstract: The present invention relates to analytical methods, platforms, and devices for the rapid and efficient immunochromatic determination of one or more components in fluid samples. The devices are especially useful for identifying analytes in small volumes of whole blood samples utilizing one membrane principally for separating particles such as red blood cells from plasma and a second membrane as the site for reactions to identify the analytes.

Abstract: A method and kit for detecting Trichomonas vaginalis infection in a human subject are disclosed. In the method, a body-fluid sample such as a vaginal-swab sample or urine is obtained from the subject and contacted with an antibody specific against a Trichomonas adhesin peptide, forming an antibody-adhesin peptide complex if the subject is infected with Trichomonas. The presence or absence of the complex establishes, with a reliability of at least 80%, in the case of a vaginal swab sample, and with a reliability of at least 40% in the case of a urine sample, the presence or absence, respectively, of Trichomonas infection in the subject. A preferred test kit employs a dry-strip, sandwich assay, format.

Abstract: The systems of the invention include test cells with a first sorbent material defining a first flow path for a solution, a second sorbent material defining a second flow path distinct from the first flow path for a sample, and a test site with immobilized antigens or antibodies or other ligand binding molecules such as aptamers, nucleic acids, etc. located at the junction of the first and second sorbent materials for identifying one or more ligands. The first and second sorbent strips touch each other at the test site location. The test cell may be used to test for pregnancy, HIV (including different HIV antigens or peptides), tuberculosis, prion, urin-analysis/drug, cardiac markers, cancer markers, Chagas, Chlamydia, dental bacteria (SM/LC), influenza A, influenza B, adenovirus, rotavirus, strep A, other bacteria or viruses, etc., and veterinary applications such as CPV, FIV, FeLV, heartworm, etc., although it is not limited to those applications.

Abstract: A sample analysis device is provided in which a target component to be analyzed is prevented from being contaminated by a sample itself, which can be formed in an appropriate size, and which has excellent operability. In a sample analysis device 1 in which a sample is to be held in a porous sheet 13, supporting films 11 and 12 are stuck on front and rear faces of the porous sheet 13, respectively, and a sample supply hole 14 is formed in a part of the supporting films.

Abstract: An apparatus, system and method of detecting contaminants, such as salmonella, in at least one ingestible item. The apparatus, system and method may include a disposable detector having therein at least one circuit layer, wherein a reaction of the reactant with at least a portion of the at least one circuit layer indicates, to the consumer user, a presence of a contaminant.

Abstract: A lateral flow test strip for the analysis of a sample featuring numerous capture zones arranged in an array such that each capture zone is substantially equidistant from the sample containing area. The sample does not pass through another capture zone to reach any one of the other capture zones. The capture zones are preferably arranged in a linear array perpendicular to the flow of the sample through the lateral flow test strip. The lateral flow test strip allows for an increased number of simultaneous analyses of numerous analytes from one sample to occur on one lateral flow test strip.

Abstract: A hinged cap for engaging a housing of a diagnostic device comprises a central body; two arms extending from opposing sides of one end of said central body, said arms being substantially parallel; each of said arms comprising a cap hinge member for engaging a housing hinge member on said housing, wherein said cap hinge member and said housing hinge member define a hinge axis around which said cap is pivotable between an open and a closed position with respect to said housing.

Abstract: Disclosed is a test strip for measuring medical data, in which a hydrophilic material is introduced to improve the spreadability of blood. Having the structure comprising a blood-filtering layer designed to filter off either or both of erythrocytes and low-density lipoprotein (LDL) cholesterol from an applied blood sample; a reaction layer in which the blood sample free of erythrocytes and/or low-density lipoprotein (LDL) cholesterol is reacted with an reagent, and a hydrophilic material, intercalated between the blood-filtering layer and the reaction layer, for uniformly spreading the blood sample filtered through the filtering layer, the blood-filtering layer and the reaction layer being stacked between the lower support and the upper cover, the test strip prevents the infiltration of erythrocytes into the reaction layer and improves the spreadability of blood to reduce measurement errors, thus bringing about higher reproducibility.

Abstract: The present invention discloses an enzyme electrode and its producing method, which comprises: providing a substrate with a carbon surface; forming a gold surface on the carbon surface and thus forming an electrode; covalently bonding a mediator containing an aldehyde group to the electrode; and covalently bonding a glucose oxidase to the electrode.

Abstract: Reagents are disclosed for use in assays for analytes. The reagents are dry assay reagents that may be readily reconstituted for use in the assays. The dry assay reagents comprise a solid support and one or more molecules of a receptor immobilized on the solid support. The receptor comprises one or more binding sites for a ligand. A portion of a total number of the binding sites is bound to a conjugate comprising the ligand linked to a specific binding pair member and a portion of the total number of the binding sites is free. In use in an assay, a combination is provided in an aqueous medium comprising the sample and reagents for detecting the analyte wherein at least one of the reagents comprises the dry assay reagent mentioned above. The combination is incubated under conditions for binding of the analyte to one or more of the reagents.

Abstract: This invention provides methods and compositions for testing for pregnancy and non-pregnancy in ungulates and non-hoofed ruminates. The tests provided by this invention are useful during a time that coincides with the estrus cycle during which breeding occurs or the first estrus cycle after breeding of a non-pregnant animal. The tests provided by this invention are useful in estrus and ovulation synchronization programs, with pregnancy testing useful at a time allowing for resynchronization of non-pregnant animals within the first estrus cycle. The tests provided by this invention assay for the presence, absence, or level of a selected IFN-?-induced protein in a sample from a female animal. The tests of this invention are useful for testing cells, blood, plasma, serum, cells, milk, nasal secretions, ocular secretions, vaginal secretions, urine, and saliva samples. The tests provided by this invention are immunoassays.

Abstract: This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications. In particular, the medical device reduces interference with an optical signal which is indicative of the presence of an analyte in a bodily sample.

Abstract: A method and system for rapidly detecting Candida on the skin of a host, such as an infant with diaper rash, is provided. The method includes contacting a dermal sample with a colorant that exhibits a certain spectral response (e.g., color change) in the presence of Candida. For example, the colorant may change from a first color to a second color, from colorless to a color, or from a color to colorless. The colorant is typically capable of differentiating between Candida (e.g., Candida albicans) and other microorganisms commonly associated with diaper rash, such as S. aureus and E. coli. Thus, when a dermal sample is placed into contact with the colorant, the color change may simply be observed to determine whether the infection is caused by Candida. If the color change occurs to a certain extent (e.g., from yellow to bright red), it may be determined that the test sample contains Candida. Likewise, if a color change occurs to a lesser extent (e.g.

Abstract: A diagnostic test kit that provides an integrated system for accurately detecting a test analyte over a broad range of possible concentrations is provided. One feature of the integrated system is that it is capable of indicating whether an analyte is within the “hook effect” region. Based on this indication, a technique may be selected for correlating a measured signal intensity to an analyte concentration or range of concentrations. For example, when it is determined that the test sample falls outside the “hook effect” region, the analyte concentration may be determined using one portion of a dose response curve. On the other hand, when it is determined that the test sample falls within the “hook effect” concentration, the analyte concentration may be determined using another portion of the dose response curve. Alternatively, the sample may simply be diluted for re-performing the assay.