Dish, Plate, Or Tray Patents (Class 435/305.1)
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Patent number: 8304228Abstract: A manipulation tool is disclosed for producing cell material having multiple biological cells, which have a predefined geometrical arrangement. The tool includes a tool body, whose surface at least partially contacts the cell material, and a setting device for adjusting the tool body by a continuous expansion, so that geometrical properties of the surface change and an interior of the tool body is enlarged. The setting device is adapted to expand the tool body at an advance velocity in a range from 0.1 ?m/h to 1 mm/h.Type: GrantFiled: March 8, 2010Date of Patent: November 6, 2012Assignee: Fraunhofer-Gesellschaft zur Foerderung der Angewandten Forschung e.V.Inventors: Günter R. Fuhr, Heiko Zimmermann
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Publication number: 20120270310Abstract: An apparatus for transfer of a liquid using a diaphragm that separates a working fluid volume from a working air volume and can controllably induce a change pressure to draw or expel a target fluid into a tube. The apparatus is particularly suitable for automated processing of nucleic acids and other samples includes a disposable container comprising a tray and a flexible barrier. The barrier is configured to seal with a top edge of the tray, providing a closed, aseptic work area within the sealed tray. A pipette head and/or other sample manipulation device can be attached to the inside of the barrier under the diaphraghm, and the barrier can include an interface for a robotic arm or other device. When the barrier is sealed over the tray, the barrier separates the contents of the tray from the robot or other manipulation device.Type: ApplicationFiled: June 7, 2012Publication date: October 25, 2012Inventors: Simon Jonathon Spence, Richard Alexander Grant, Timothy Doyle Peele, William Samuel Hunter, Ashraf F. Abdelmoteleb, David Thomas Kneen, Robert Alister Neil, Simon Harris
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Publication number: 20120252105Abstract: Cultivators and methods of cultivating microorganisms are provided. In some examples, a cultivator may include a retaining wall defining a cavity for retaining liquid and may also include a horizontally orientated frame at least partially positioned within the cavity and having media for supporting microorganisms. In other examples, the horizontal frame may be partially submerged in the liquid retained in the cavity and rotatable to selectively submerge and unsubmerge portions of the frame and media.Type: ApplicationFiled: September 22, 2011Publication date: October 4, 2012Applicant: BIOPROCESSH20 LLCInventors: Toby D. Ahrens, Shawn R. Kitchner, John W. Haley, III
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Patent number: 8273312Abstract: A liquid testing assembly for testing a liquid, the assembly comprising a test vessel and a stopper adapted to fit into a free end of the vessel. The stopper substantially hermetically seals the test vessel from the ambient. Further, the assembly includes a support coated with one or more identifying materials for identifying one or more constituents of the liquid. The support is fixed in the stopper and/or the vessel and extends into its interior for a predetermined distance. The liquid testing assembly when assembled is pre-evacuated to a predetermined vacuum sufficient to draw a predetermined volume of liquid to be sampled into the test vessel. The predetermined volume is of such an amount that it wets the one or more identifying materials ensuring identification of one or more constituents present in the liquid. A kit employing the liquid testing assembly is also discussed.Type: GrantFiled: January 22, 2008Date of Patent: September 25, 2012Assignee: Association for Public HealthInventors: Gadi Porat, Joel Stern, Boaz Rimon, Yoram Cohen
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Patent number: 8263391Abstract: The invention relates to a specimen carrier for the study of cell growth, comprising a substrate with a reservoir with a bottom, wherein the reservoir is filled up to a predetermined height of the side wall, which is smaller than the complete height of the reservoir, with a carrier material for cell growth and the side wall of the reservoir is formed such that a predetermined contact angle of the carrier material can be adjusted with respect to the side wall.Type: GrantFiled: July 19, 2007Date of Patent: September 11, 2012Assignee: ibidi GmbHInventors: Roman Zantl, Valentin Kahl
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Publication number: 20120214230Abstract: The present invention relates to a fast a simple coating procedure for coating of cell culture and/or handling surfaces to prevent cell adhesion and growth. The invention relates to a cell culture/handling product coated with phenyl dextran, as well as methods of producing and using it.Type: ApplicationFiled: October 21, 2010Publication date: August 23, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Cecilia Anneren, Gunnar Glad, Christine Sund Lundstrom
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Publication number: 20120208761Abstract: It is an object of the present invention to provide a Type I-Type IV collagen hybrid gel, which maintains characteristics of a Type IV collagen and is superior in gel strength. It is the Type I-Type IV collagen hybrid gel obtained by mixing 100 to 500 parts by mass of the Type I collagen having fibrosis ability, relative to 100 parts by mass of the Type IV collagen having gelling ability. A three-dimensional structure is formed, where a membrane-like substance by the Type IV collagen is formed onto a fibrous substance by the Type I collagen, so as to be able to provide cell culture environment approximate to a basement membrane of a living body.Type: ApplicationFiled: February 14, 2011Publication date: August 16, 2012Inventors: Shunji Hattori, Yoh-ichi Koyama, Hitomi Fujisaki, Kiyotoshi Sekiguchi, Sugiko Futaki, Ryoko Sato
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Publication number: 20120208266Abstract: Disclosed herein are cell culture surfaces comprising a substrate and a polymer layer on the substrate where the polymer layer contains enzyme-cleavable amino acid sequences and cell adhesive peptide sequences.Type: ApplicationFiled: February 7, 2012Publication date: August 16, 2012Inventors: Dana Craig Bookbinder, Arthur Winston Martin, Jodelle Karen Nelson, Shawn Michael O'Malley, Meenal Pauddar Soni
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Publication number: 20120183510Abstract: A method and device for creating hanging drop cell aggregates. The method and device includes a plurality of pegs that allow for high throughput culture of aggregates. Also disclosed are means of transferring formed aggregates to various destinations, such as well plates, scaffolding, tissues, or wounds. Use of the device permits aggregates to be prepared or created in larger quantities than current methods, and allows for them to be transferred more efficiently.Type: ApplicationFiled: June 30, 2010Publication date: July 19, 2012Applicant: University of Virginia Patent FoundationInventors: Blair Taylor Stocks, Shayn Peirce Cottler, Adam J. Katz
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Publication number: 20120184029Abstract: The present invention relates to preparation of a polymer coating comprising or consisting of polymer chains comprising or consisting of units of 2-methoxyethyl acrylate synthesized by Surface-Initiated Atom Transfer Radical Polymerization (SI ATRP) such as ARGET SI ATRP or AGET SI ATRP and uses of said polymer coating.Type: ApplicationFiled: July 14, 2010Publication date: July 19, 2012Applicant: Technical University of DenmarkInventors: Katja Jankova Atanasova, Soren Hvilsted, Charlotte Juel
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Patent number: 8222026Abstract: A membrane supported bioreactor arrangement and method for anaerobic conversion of gas into liquid products including membrane modules having hollow fibers packed across a cross sectional area of the membrane module, each of the hollow fibers formed from an asymmetric membrane wall having a porous outer layer defining biopores for retaining a porous biolayer about the outer surface of the membrane wall and a less permeable hydration layer around the hollow fiber lumen; a membrane vessel for surrounding the outside of the hollow fibers with a process gas from a gas supply conduit; and a liquid supply conduit operably connected to the hollow fibers for supplying a process liquid to the hollow fiber lumens. The gas supply conduit enables the formation of a biolayer on the outer surface of the hollow fiber wall by interaction of microorganisms with the process gas and the production of a liquid product.Type: GrantFiled: October 24, 2008Date of Patent: July 17, 2012Assignee: Coskata, Inc.Inventors: Shih-Perng Tsai, Rathin Datta, Rahul Basu, Seong-Hoon Yoon
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Publication number: 20120156777Abstract: A carrier for growing adherent cells is provided, wherein the carrier comprises one or more outer surfaces; and one or more structured indentations on one or more of the outer surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. The carrier may comprise a single indentation or ‘cup’ like structure, or may comprise a plurality of indentations. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.Type: ApplicationFiled: December 16, 2010Publication date: June 21, 2012Applicant: GENERAL ELECTRIC COMPANYInventors: Arvind Rangarajan, Prameela Susarla, Scott Michael Miller, Reginald Donovan Smith, Slawomir Rubinsztajn, Anton Beletskii
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Publication number: 20120156772Abstract: In one example of a carrier for growing adherent cells, comprises one or more surfaces; and one or more relief features on one or more of the surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.012 mm to 0.5 mm; and wherein each of the relief features has a height in a range from about 2 to 200 ?m, and width in a range from about 20 to 200 ?m.Type: ApplicationFiled: November 2, 2011Publication date: June 21, 2012Applicant: GENERAL ELECTRIC COMPANYInventors: Scott Michael Miller, Yosang Yoon, Slawomir Rubinsztajn, Peter Kennedy Davis, Prameela Susarla
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Publication number: 20120134963Abstract: Provided are viral sensitizing compounds that enhance the efficacy of viruses by increasing spread of the virus in cells, increasing the titer of virus in cells, or increasing the cytotoxicity of virus to cells. Other uses, compositions and methods of using same are also provided.Type: ApplicationFiled: July 7, 2010Publication date: May 31, 2012Applicant: Ottawa Hospital Research InstituteInventors: John Bell, Jean-Simon Diallo
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Publication number: 20120135513Abstract: The invention relates to a bioreactor for cultivating phototrophic organisms in an aqueous culture medium, the reactor parts that come into contact with the culture medium being entirely or partially produced from silicone materials. The invention is characterized in that the silicone materials are produced from addition-crosslinked silicones, and the surface of the silicone materials has a contact angle to the water of at least 100°.Type: ApplicationFiled: August 6, 2010Publication date: May 31, 2012Inventors: Christoph Müller-Rees, Rupert Pfaller
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Publication number: 20120135514Abstract: The invention relates to a bioreactor for cultivating phototrophic organisms in an aqueous culture medium. The reactor parts and/or fittings that come into contact with the culture medium are entirely or partially coated with a silicone layer, and the surface of the silicone layer has a contact angle to the water of at least 100°.Type: ApplicationFiled: August 6, 2010Publication date: May 31, 2012Applicant: Wacker Chemie AGInventors: Christoph Muller-Rees, Rupert Pfaller
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Patent number: 8177082Abstract: An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly includes a flexible membrane, a contiguous side wall, an end wall, and a microbial filter. The end wall has an opening and extends across one end of the side wall to form a major cavity within the side wall and the end wall. The flexible membrane has a slit spanning the membrane and is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the end wall and the membrane and (ii) a minor cavity formed by the membrane and a portion of the side wall. The minor cavity is in fluid communication with the reservoir.Type: GrantFiled: April 18, 2008Date of Patent: May 15, 2012Assignee: Corning IncorporatedInventors: Henry Joseph Cattadoris, Gregory Roger Martin, Allison Jean Tanner
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Patent number: 8168432Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.Type: GrantFiled: February 17, 2011Date of Patent: May 1, 2012Assignee: Wilson Wolf ManufacturingInventors: John R. Wilson, Douglas A. Page, Dan Welch, Alison Robeck
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Patent number: 8163537Abstract: A nested permeable support device is described herein that can be used to perform various experiments to test new therapeutic compounds (new chemical entities). In one application, the nested permeable support device is used to perform a first pass assay to determine the bioavailability of a new chemical entity following absorption through the digestive tract and metabolism by the liver.Type: GrantFiled: April 8, 2009Date of Patent: April 24, 2012Assignee: Corning IncorporatedInventors: Gregory R. Martin, Allison J. Tanner
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Patent number: 8158426Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.Type: GrantFiled: July 29, 2011Date of Patent: April 17, 2012Assignee: Wilson Wolf Manufacturing CorporationInventors: John R. Wilson, Douglas A. Page, Dan Welch, Alison Robeck
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Patent number: 8158427Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.Type: GrantFiled: July 29, 2011Date of Patent: April 17, 2012Assignee: Wilson Wolf Manufacturing CorporationInventors: John R. Wilson, Douglas A. Page, Dan Welch, Alison Robeck
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Patent number: 8158407Abstract: Hybrid synthetic grafts and embodiments of systems and methods for producing hybrid vascular grafts that can yield implantable grafts that combine synthetic grafts with living cells. Embodiments of systems can include a pressure/flow loop subsystem having an external flow loop system coupled to a specimen holder, where the pressure/flow loop subsystem is capable of adjusting at least two dynamic conditions in the specimen holder or a diameter of a specimen in the specimen holder. Embodiments of methods can coat a hybrid graft with a confluent monolayer of endothelial cells by immobilizing stem cells on a hybrid hemodialysis access graft or a hybrid femoral artery bypass graft, and placing the hybrid graft in a system embodiment according to the invention under conditions effective to promote the stem cells to form a confluent monolayer on the hybrid graft and in an environment to promote the stem cells to differentiate into endothelial cells.Type: GrantFiled: January 24, 2008Date of Patent: April 17, 2012Assignee: ICE Development Technologies, LLCInventor: Michael Dancu
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Publication number: 20120077259Abstract: We describe a thermal cycler comprising a Peltier-type thermoelectric element used for cooling a sample block, and a non-Peltier-type heating device for heating the sample block. The cycler also includes a heat sink connected to the Peltier-type element by a heat pipe, which permits thermal energy to transfer from the Peltier-type element to the heat sink. This configuration operates more efficiently than conventional thermal cyclers which use Peltier-type elements for heating and cooling, and allows a more rapid cycling time as well as operation in a wider range of ambient temperatures. Certain embodiments utilise the Peltier-type element as a thermal gate to reduce thermal loss during heating when the Peltier-type element is switched off.Type: ApplicationFiled: September 22, 2011Publication date: March 29, 2012Applicant: EPISTEM LIMITEDInventor: Ben COBB
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Publication number: 20120064046Abstract: Various embodiments of the present invention include compositions, materials and methods for maintaining and propagating mammalian mesenchymal stem cells in an undifferentiated state in the absence of feeder cells and applications of the same.Type: ApplicationFiled: November 16, 2011Publication date: March 15, 2012Applicants: THE UNITED STATES OF AMERICA AS REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS, THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSASInventors: Xiao-Dong Chen, Robert L. Jilka
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Patent number: 8129179Abstract: A bioreactor (200) for cultivating living cells in a liquid medium. In one embodiment, the bioreactor (200) has a substrate (230) having a first surface and an opposite second surface, defining a channel (202) therein, and a plurality of chambers (204) formed in the substrate, wherein each of the plurality of chambers (204) is adapted for receiving cells in a liquid medium and formed with an open end (262), an opposite closed end (264) and side walls, the open end (262) and the closed end (264) defining a depth, d, therebetween for the corresponding chamber (206), the sidewalls defining a width, w, therebetween for the corresponding chamber (206). As such formed, each chamber (206) is in fluid communication with the channel (202) through the open end of the chamber (206), and at least two of the plurality of chambers (206) have depths or widths the same or different form each other.Type: GrantFiled: August 27, 2003Date of Patent: March 6, 2012Assignee: Vanderbilt UniversityInventors: John F. Wikswo, David Cliffel, Eugene J. Leboeuf, Randall S. Reiserer
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Publication number: 20120052565Abstract: A cell culture article has substrate that is predominantly opaque and that provides a three-dimensional (e.g., irregular) surface, but incorporates an optically transparent, substantially regular (e.g., two-dimensional) surface to serve as a microscopic observation and imaging window for the 3D cell culture. In many embodiments, the 3D portion of the substrate occupies greater than 99% of the surface while the 2D portion occupies less than 1% of the surface so as not to substantially disrupt the general 3D culture environment.Type: ApplicationFiled: August 25, 2011Publication date: March 1, 2012Inventors: Michael Edward DeRosa, Joydeep Lahiri, Hongwei Hanna Rao, Po Ki Yuen
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Publication number: 20120003686Abstract: The present disclosure describes holder devices, testing kits that include the holder devices, and methods for using the holder devices to allow a user to dispense an aqueous test sample from a sampling device onto a culture plate device having a cover sheet, whereby the cover sheet is supported in an open position by the holder while the user dispenses the aqueous test sample onto a base member of the culture plate device.Type: ApplicationFiled: June 3, 2011Publication date: January 5, 2012Inventors: Manjiri T. Kshirsagar, Kurt J. Halverson, James E. Aysta
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Patent number: 8088573Abstract: Apparatus and methods for carrying out biochemical processes directly on a substrate are provided. A substrate assembly includes a cartridge that removably supports a substrate in a fixed position, and a reaction containment member that is removably located on top of the substrate. The reaction containment member includes one or more cavities that form chambers directly above one or more target locations on the surface of the substrate. The chambers can be used to conduct biochemical processes directly over the substrate, as well as to perform thermal cycling of material contained inside the chamber using a heating element disposed directly on the substrate. The substrate assembly is preferably used in combination with a processing machine that dispenses materials into the chambers and that conducts biochemical reactions of materials contained within the chambers, without requiring the substrate assembly to be moved from one location to another location during the processes.Type: GrantFiled: April 25, 2008Date of Patent: January 3, 2012Assignee: Sequenom, Inc.Inventors: Paul Heaney, Chao Lin, David Opalsky, Phillip Bruce, III, Charles Griswold, Sheila A. Walker, Ralf Worl, Andrzej Maczuszenko
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Publication number: 20110318825Abstract: An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. Each treatment step occurs within the same reaction compartment. The reaction conditions of each reaction compartment for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide. The reagents are preferably held in a reagent dispensing strip similar to a “blister pack”.Type: ApplicationFiled: August 29, 2011Publication date: December 29, 2011Inventor: Lee Angros
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Patent number: 8080412Abstract: A continuous temperature-gradient incubation apparatus designed to solve the problem of moving of liquid vapor generated on the high-temperature side toward the low-temperature side to thereby cause condensation.Type: GrantFiled: April 13, 2007Date of Patent: December 20, 2011Assignee: Research Organization of Information and SystemsInventor: Nobuo Shimamoto
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Publication number: 20110306136Abstract: A cell culture system including: a substrate, a substrate coating, one or more live cells, and an overlay source. The substrate coating and overlay can be laminin, and laminin•entactin complex, respectively. Alternatively, the substrate coating and overlay can be laminin•entactin complex, and laminin, respectively. The cell culture system can further include liquid media and a protective cover. A method for making and using the system in cell culture articles and culture methods, as defined herein, is also disclosed.Type: ApplicationFiled: May 19, 2011Publication date: December 15, 2011Inventors: Theresa Chang, Jin Liu, Odessa Natalie Petzold, Ruchirej Yongsunthon
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Publication number: 20110306134Abstract: A method for promoting the retention of a stem cell phenotype in a population of stem cells. A biocompatible substrate is provided, e.g. in the form of cultureware. The substrate has an arrangement of topographical features arrayed in a pattern based on a notional symmetrical lattice in which the distance between nearest neighbour notional lattice points is between 10 nm and 10 ?m, and wherein the topographical features are either located in register with the respective notional lattice points or are locally misordered such that the centre of each topographical feature is at most 10% of the distance between nearest neighbour notional lattice points from its respective notional lattice point. A population of stem cells is provided in contact with said arrangement of topographical features. Culturing the population of stem cells under conditions that allow the stem cells to proliferate allows the retention of the stem cell phenotype in the population of stem cells.Type: ApplicationFiled: February 23, 2010Publication date: December 15, 2011Inventors: Matthew Dalby, Nikolaj Gadegaard, Richard Oreffo
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Publication number: 20110306122Abstract: A hanging drop plate and a method of cultivating cells or of producing molecular aggregates in at least one liquid volume that adheres to a drop contact area of such a hanging drop plate. The hanging drop plate has a body with a first surface and a second surface that is essentially coplanar to the first surface. The second surface has a drop contact area for adherently receiving a liquid volume. The drop contact area is distinguished from a surrounding area by a relief structure that prevents spreading of the liquid volume on the second surface of the body. The body has at least one conduit that mouths into the drop contact area from the direction of the first surface of the body. A liquid volume is applied to the drop contact area through a communicating conduit. Cells and/or molecules can be introduced into this liquid volume.Type: ApplicationFiled: September 22, 2008Publication date: December 15, 2011Applicant: Universitat Zurich Prorektorat ForschungInventors: Wolfgang Moritz, Jens Kelm, Pierre-Alain Clavien, Simon Philip Hoerstrup
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Patent number: 8058060Abstract: The present invention relates to a culture insert carrier, a culture insert and a culture system for culturing and testing of different kinds of cells, such as for example skin models. A culture insert carrier for supporting at least one culture insert in a culture tray having at least one well, wherein the culture insert carrier comprises a plane member with at least one opening for insertion of the at least one culture insert in such a way that the culture insert carrier supports the at least one culture insert in a position in the culture tray is provided. Further, a culture insert comprising a plurality of suspension elements including a first set of suspension elements and a second set of suspension elements, the suspension elements being adapted to suspend the culture insert in a plurality of vertical positions, including a first vertical position and a second vertical position, in relation to a frame is provided.Type: GrantFiled: June 9, 2006Date of Patent: November 15, 2011Assignee: Nunc A/SInventors: Peter Esser, Frank T. Stigborg, Klaus Pedersen, Stefan Iskov
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Publication number: 20110263011Abstract: Method for providing a substrate with an anti-biofouling coating the method comprising: a. obtaining a coating composition comprising nanoparticles being grafted with reactive groups and hydrophilic polymer chains and a solvent; b. applying the coating composition to the substrate; and c. optionally curing the coating composition herein the surface tension of the coating composition at 25° C. is below 40 mN/m.Type: ApplicationFiled: October 30, 2009Publication date: October 27, 2011Inventors: Jun Qiu, Ronnie B.M. de Rijk, Jens Christoph Thies
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Patent number: 8043849Abstract: Multi-layer devices suitable for thermal cycling processes. The devices are particularly suitable for performing polymerase chain reactions (PCR). One embodiment includes a first conducting layer, a second conducting layer adjacent to the first layer, and a third conducting layer adjacent to the second layer opposite the first layer. Insulating layers are positioned between said three conducting layers. Continuous channels are formed within the layers. The channels can be formed in either the conducting layer or the insulating layers, or both. Other embodiments include two conducting layers. At least one integral or separate temperature source may be provided to maintain the conducting layers at various desired temperatures.Type: GrantFiled: May 4, 2007Date of Patent: October 25, 2011Assignee: Thermal GradientInventor: Joel W. Grover
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Patent number: 8039252Abstract: A multi-chamber cell culture assembly has provisions for the distribution of nutrient culture medium and gasses throughout each of the chambers. A device is constructed to provide a large surface area for the growth and cultivation of hybridomas, mammalian and insect cells. The device may incorporate macro, micro or nano structures on the growth surfaces to promote or enhance distribution of nutrients, cell product, gasses or growth area. Cell growth, nutrient addition and cell product withdrawal may be carried out automatically.Type: GrantFiled: January 11, 2007Date of Patent: October 18, 2011Inventor: Eric Berry
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Patent number: 8039246Abstract: Hybrid synthetic grafts and embodiments of systems and methods for producing hybrid vascular grafts that can yield implantable grafts that combine synthetic grafts with living cells. Embodiments of systems can include a pressure/flow loop subsystem having an external flow loop system coupled to a specimen holder, where the pressure/flow loop subsystem is capable of adjusting at least two dynamic conditions in the specimen holder or a diameter of a specimen in the specimen holder. Embodiments of methods can promote endothelialization of a hybrid hemodialysis access graft or a hybrid femoral artery bypass graft by placing the hybrid hemodialysis access graft or the hybrid femoral artery bypass graft in a system embodiment according to the invention under conditions effective to promote endothelial cells to form a confluent monolayer on the surface of the hybrid graft.Type: GrantFiled: January 24, 2008Date of Patent: October 18, 2011Assignee: ICE Development Technologies, LLCInventor: Michael Dancu
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Patent number: 8034608Abstract: Hybrid synthetic grafts and embodiments of systems and methods for producing hybrid vascular grafts that can yield implantable grafts that combine synthetic grafts with living cells. Embodiments of systems can include a pressure/flow loop subsystem having an external flow loop system coupled to a specimen holder, where the pressure/flow loop subsystem is capable of adjusting at least two dynamic conditions in the specimen holder or a diameter of a specimen in the specimen holder. Embodiments of methods can promote endothelialization of a hybrid hemodialysis access graft or a hybrid femoral artery bypass graft by placing the hybrid hemodialysis access graft or the hybrid femoral artery bypass graft in a system embodiment according to the invention under conditions effective to promote stem cells to form a confluent monolayer on the hybrid graft.Type: GrantFiled: January 24, 2008Date of Patent: October 11, 2011Assignee: ICE Development Technologies, LLCInventor: Michael Dancu
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Publication number: 20110206643Abstract: A method for automated cell processing is provided, including receiving at least one tissue containing a multiplicity of cells belonging to multiple cell types, and automatically increasing both the proportion of cells of at least one of said multiple cell types as compared with at least another of said multiple cell types and the absolute number of cells of said at least one of said multiple cell types. Other embodiments are also described.Type: ApplicationFiled: May 31, 2006Publication date: August 25, 2011Applicants: IN MOTION INVESTMENT, LTD.Inventors: Valentin Fulga, Robert Michael Clark, Yael Porat, Danny Belkin, Avner Schrift, Eyal Teichman
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Patent number: 7998730Abstract: A method and apparatus are provided for transferring growth media or infection fluids to a bioculture bag having Could Process fitting thereon in fluid communication with the inside of the bag. The media or fluid is placed in a flask. A cap is threaded onto the neck of the flask. The cap has a spout with an opening in a distal end of the spout and a vent hole in the cap. The spout has a tubular end in which the opening is formed, with an O-ring seal adjacent the distal end of the tubular end. The tubular end fits within the fitting and the seal forms a fluid tight seal with the fitting to allow fluid transfer to the bag in reduced time with reduced spillage. The cap is preferably used on a flask having shaped baffles in the bottom. The baffles are highest toward the centerline, and decline in height linearly toward the corners of the flask at an angle of about 9° to the horizontal.Type: GrantFiled: April 30, 2010Date of Patent: August 16, 2011Assignee: Securus, Inc.Inventors: Samuel A. Ellis, Kenneth E. Holmes
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Patent number: 7981668Abstract: A method and system for culturing cells, having a substantially airtight enclosure configured to culture cells. The method and system also have a first conduit configured to provide a reduced pressure to the substantially airtight enclosure and a second conduit configured to provide a culture media to the substantially airtight enclosure.Type: GrantFiled: January 17, 2007Date of Patent: July 19, 2011Assignee: KCI Licensing Inc.Inventors: Robert P. Wilkes, Amy K. McNulty, Kristine Kieswetter, Teri D. Feeley, Marisa Schmidt
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Patent number: 7977089Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier dividing the chamber into a first subchamber and a second subchamber, wherein the barrier has a porosity to allow the first subchamber and the second subchamber in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber and the second subchamber.Type: GrantFiled: August 27, 2003Date of Patent: July 12, 2011Assignee: Vanderbilt UniversityInventors: John P. Wikswo, Franz J. Baudenbacher, David Cliffel, Frederick R. Haselton, Eugene J. Leboeuf, Ales Prokop, Randall S. Reiserer, Mark A. Stremler
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Patent number: 7972841Abstract: The present invention relates to an exposure device for living cell cultures, the device having a medium chamber common to a plurality of cell culture chambers and medium directing means. The medium chamber, cell culture chambers and the medium directing means may be arranged so as to provide substantially contemporaneous medium exchange at the cell culture chambers.Type: GrantFiled: May 22, 2003Date of Patent: July 5, 2011Assignee: British American Tobacco (Investments) LimitedInventors: Elan David Massey, Justine Williamson, Jeremy Francis Neale Phillips
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Patent number: 7968329Abstract: A method of yielding a functional human hybrid coronary bypass graft is provided. The method includes conditioning a hybrid synthetic tubular structure having stem cells and/or endothelial cells on at least one surface to yield the functional human hybrid coronary bypass graft. Specifically, the method includes placing the hybrid synthetic tubular structure in a system capable of producing three dimensional dynamic conditions for a sufficient time to yield said functional human hybrid coronary bypass graft.Type: GrantFiled: December 17, 2007Date of Patent: June 28, 2011Inventor: Michael Dancu
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Patent number: 7964387Abstract: A method of yielding a functional human hybrid hemodialysis access graft is provided. The method includes conditioning a hybrid synthetic tubular structure having stem cells and/or endothelial cells on at least one surface to yield the functional human hybrid hemodialysis access graft. Specifically, the method includes placing the hybrid synthetic tubular structure in a system capable of producing three dimensional dynamic conditions for a sufficient time to yield said functional human hybrid hemodialysis access graft.Type: GrantFiled: December 19, 2007Date of Patent: June 21, 2011Inventor: Michael Dancu
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Publication number: 20110129812Abstract: An algal production system that uses CO2 injections to promote the growth of algae. The system includes an algal growth medium within a floway for channeling water. A fluid diffuser also resides in the floway in proximity to the algal growth medium. The fluid diffuser injects CO2 into the water in the floway. The system further includes a detector for monitoring the pH levels of the water and a controller, which based on the measured pH levels, determines when and how much CO2 to inject into the water.Type: ApplicationFiled: November 19, 2010Publication date: June 2, 2011Inventors: Walter H. Adey, Erik T. Adey
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Publication number: 20110111504Abstract: The invention relates to a bioreactor (1) for cell and tissue culture for the mechanical stimulation and for perfusion of tissue or cell cultures, comprising an upper part (3) and a lower part (4) which is connected to said upper part (4) and which comprises a tissue culture chamber (8), wherein at least one distributor plate (12) is arranged in the tissue culture chamber (8), and a method for cultivating cells and tissues in the bioreactor (1).Type: ApplicationFiled: May 25, 2009Publication date: May 12, 2011Applicant: Greiner Bio - One GmbHInventors: Guenther Knebel, Sven Muehlfriedel
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Publication number: 20110097790Abstract: A lockable cell growth chamber is disclosed wherein the lid and base of the dish are provided with at least two pairs of locking and anti-locking members, the locking and antilocking functions being activated by application of rotational torque.Type: ApplicationFiled: October 26, 2009Publication date: April 28, 2011Inventor: Patrick Yerbic
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Patent number: 7932082Abstract: A device is disclosed for extracting a smear sample. The device includes a cavity, into which a sample carrier carrying a smear sample can be introduced. Liquid can be introduced into the cavity through at least one liquid feed connected to the cavity, and the liquid can be removed from the cavity through at least one liquid discharge connected to the cavity.Type: GrantFiled: November 15, 2006Date of Patent: April 26, 2011Assignee: Siemens AktiengesellschaftInventors: Klaus Abraham-Fuchs, Thomas Ehben, Walter Gumbrecht, Peter Paulicka, Sebastian Schmidt, Manfred Stanzel, Christian Zilch