Human Patents (Class 435/366)
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Patent number: 10377989Abstract: The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation.Type: GrantFiled: December 30, 2013Date of Patent: August 13, 2019Assignee: Janssen Biotech, Inc.Inventors: Benjamin Fryer, Daina Laniauskas, Marcia Blackmoore, Haiyun Wang, Kostadinka LiLova, Shelley Nelson, Elizabeth Rosocha
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Patent number: 10370644Abstract: The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.Type: GrantFiled: June 17, 2014Date of Patent: August 6, 2019Assignee: Janssen Biotech, Inc.Inventors: Benjamin Fryer, Daina Laniauskas, Marcia Blackmoore, Haiyun Wang, Kostadinka Lilova, Shelley Nelson, Elizabeth Rosocha
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Patent number: 10363341Abstract: The present invention relates to a tissue-engineered intervertebral disc (IVD) suitable for total disc replacement in a mammal and methods of fabrication. The IVD comprises a nucleus pulposus structure comprising a first population of living cells that secrete a hydrophilic protein and an annulus fibrosis structure surrounding and in contact with the nucleus pulposus structure, the annulus fibrosis structure comprising a second population of living cells and type I collagen. The collagen fibrils in the annulus fibrosis structure are circumferentially aligned around the nucleus pulposus region due to cell-mediated contraction in the annulus fibrosis structure. Also disclosed are methods of fabricating tissue-engineered intervertebral discs.Type: GrantFiled: April 26, 2017Date of Patent: July 30, 2019Assignee: Cornell UniversityInventors: Lawrence J. Bonassar, Roger Hartl, Robert D. Bowles, Harry H. Gebhard
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Patent number: 10351827Abstract: There is provided a method of differentiating an induced pluripotent stem cell (iPSC) into a renal proximal tubular cell (PTC)-like cell. The method comprises culturing an undifferentiated iPSC in a renal epithelial cell culture medium in the presence of one or more extracellular matrix (ECM) molecules, bone morphogenic protein 2 (BMP2) and bone morphogenic protein 7 (BMP7), for a period of from about 8 to about 10 days, under conditions sufficient to induce differentiation of the iPSC into a PTC-like cell. A cell population of differentiated PTC-like cells is also provided, as well as uses and methods of use of the cell population.Type: GrantFiled: November 11, 2014Date of Patent: July 16, 2019Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCHInventors: Daniele Zink, Jackie Y. Ying
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Patent number: 10336980Abstract: A portable apparatus useful for collection and processing of human biological material, such as adipose or cancellous bone material, to prepare a concentrated product (e.g., stromal vascular fraction). The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a pellet well for collecting concentrate product in the form of a pellet phase from centrifuge processing. The pellet well is accessible only from above when the apparatus is in an access orientation. Collected pellet phase material may be removed from the pellet well by direct aspiration, without suspending the material in a suspension liquid within the container. Access ports may be configured for access only from above the container. The apparatus may include a tissue collector disposed in the disuse retention volume to engage and collect collagen or other stringy tissue.Type: GrantFiled: September 5, 2014Date of Patent: July 2, 2019Assignee: The GID Group, Inc.Inventors: William W. Cimino, Ramon Llull, Adam J. Katz
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Patent number: 10337009Abstract: The invention provides a single-stranded nucleic acid molecule of (A) or (B) containing a TGF-?1 gene expression inhibitory sequence. The single-stranded nucleic acid molecule (A) consists of region (Xc), linker region (Lx) and region (X) from the 5?-side to the 3?-side, wherein linker region (Lx) has a non-nucleotide structure containing at least one of a pyrrolidine skeleton and a piperidine skeleton, and at least one of region (X) and region (Xc) contains the expression inhibitory sequence.Type: GrantFiled: December 15, 2015Date of Patent: July 2, 2019Assignee: BONAC CORPORATIONInventors: Takahiro Matsumoto, Hidekazu Toyofuku
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Patent number: 10321704Abstract: The present application relates to propionic grain-based compositions comprising lactic acid-producing bacteria.Type: GrantFiled: April 13, 2017Date of Patent: June 18, 2019Assignee: Ganeden Biotech, Inc.Inventors: Sean Farmer, Andrew R. Lefkowitz, Michael Bush, David Maske
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Patent number: 10287549Abstract: Provided is a muscle stem cell in vitro culture method. A muscle stem cell is cultivated in vitro by using a cell culture medium of a cell factor added with a blood cell or a conditioned medium of the blood cell. Also provided is a culture medium used in the muscle stem cell in vitro culture method and an application thereof.Type: GrantFiled: May 21, 2014Date of Patent: May 14, 2019Assignee: Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesInventors: Ping Hu, Xin Fu, Jun Xiao
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Patent number: 10278993Abstract: A method for the treatment of acute stroke in a subject by administering to said subject an effective amount of a cell-based composition containing a suspension of mesenchymal stem cells in crystalloid with a cellular concentration from 0.01 million to 3.0 million cells/ml.Type: GrantFiled: May 17, 2017Date of Patent: May 7, 2019Assignee: Cytopeutics Sdn. Bhd.Inventors: Bernard Sze-Piaw Chin, Kong Yong Then, Soon Keng Cheong
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Patent number: 10273459Abstract: The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells. The invention system is useful for isolating somatic cells from tissue samples, producing iPSC lines from adult differentiated cells by reprogramming such cells, identifying the pluripotent reprogrammed adult cells among other cells, and expanding and screening the identified reprogrammed cells.Type: GrantFiled: November 30, 2012Date of Patent: April 30, 2019Assignee: New York Stem Cell FoundationInventors: Scott Noggle, Kevin C. Eggan, Stephen Chang, Susan Solomon
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Patent number: 10190097Abstract: The present invention provides a method of forming a fibromodulin (FMOD) reprogrammed (FReP) cell. The method comprises steps of treating a human cell with a cell culture medium comprising fibromodulin (FMOD) for a period ranging from a day to a month, and changing the cell culture medium regularly until a FMOD reprogrammed (FreP) cell forms; wherein the FreP cell expresses NANOG and does not form teratoma, and wherein the human cell is a fibroblastic cell.Type: GrantFiled: December 19, 2016Date of Patent: January 29, 2019Assignee: The Regents of the University of CaliforniaInventors: B. Chia Soo, Kang Ting, Zhong Zheng
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Patent number: 10179152Abstract: Provided herein are methods of treatment comprising administering to a subject, e.g., a human subject, mononuclear cells from human placental perfusate and hematopoietic cells, and compositions comprising them, and their uses to establish chimerism, engraft tissue (e.g., blood), reduce the severity or duration of graft versus host disease, and treat or ameliorate symptoms of metabolic disorders and hematologic disorders, such as hematologic malignancies.Type: GrantFiled: October 2, 2014Date of Patent: January 15, 2019Assignee: Celularity, Inc.Inventors: Jodi P. Gurney, Xiaokui Zhang, Stacy Herb, Robert J. Hariri
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Patent number: 10167449Abstract: An isolated human brown adipose tissue stem cell line. In one embodiment, the isolated human brown adipose tissue stem cell line expresses the markers CD9, SSEA4, CD44, CD90, CD166, CD73, but not CD14, CD34, CD45 or STRO-1. In another embodiment, the isolated human brown adipose tissue stem cell line expresses the genes UCP1, PPARGC1A, NRF1, FOXC2, CREB1, SIRT3, and WNT5A (REFX). In still another embodiment, the isolated human brown adipose tissue stem cell line is capable of differentiating into osteoblasts, chondrocytes, and adipocytes.Type: GrantFiled: April 17, 2014Date of Patent: January 1, 2019Assignee: BIORESTORATIVE THERAPIES, INC.Inventor: Francisco Javier Silva
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Patent number: 10155927Abstract: This application relates to a method for differentiating pluripotent stem cells (PSCs) into cardiomyocytes. Moreover this application relates to a method for differentiating human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) into defined cardiomyocytes based on linked steps of chemically defined medium inductions.Type: GrantFiled: May 6, 2015Date of Patent: December 18, 2018Assignee: HOFFMANN-LA-ROCHE INC.Inventors: Mark Burcin, Sonja Schlicht
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Patent number: 10150946Abstract: The present invention relates to a medium for storing, preserving, culturing, and/or differentiating stem cells. The medium employs a synthetic thermo-responsive copolymer, dispersed in an aqueous vehicle, which allows the medium to be readily “switched” between a non-gelatinous (fluid) form and a gelatinous form by simply adjusting temperature across the gelling temperature of the medium. As such, stem cells can be easily captured within a 3D gel matrix by simply mixing the stem cells with the non-gelatinous/fluid form of the medium and then gelling the medium by adjusting the temperature across the gelling temperature of the medium. The stem cells encapsulated within the gelatinous form of the medium may then be preserved for a significant time period before either using the stem cells directly within the medium (e.g. in therapy, or in culturing, differentiation, and such like), or after their extraction from the medium.Type: GrantFiled: February 21, 2014Date of Patent: December 11, 2018Assignee: The University of SheffieldInventors: Irene Canton, Steven Peter Armes, Nicholas John Warren, Harry Moore, Giuseppe Battaglia, Denis Cecchin
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Patent number: 10138463Abstract: The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.Type: GrantFiled: November 24, 2014Date of Patent: November 27, 2018Assignee: VIACYTE, INC.Inventor: Thomas C Schulz
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Patent number: 10106773Abstract: The present invention relates to methods of isolating a substantially homogeneous population of pluripotent stem cells from adult neural crest tissue (e.g., periodontal ligament) as well as pharmaceutical compositions comprising such isolated pluripotent stem cells. Methods of inducing the isolated pluripotent stem cells into specific cell lineages, such as neurogenic and retinogenic lineages, are also described. The isolated pluripotent stem cells find use in various regenerative medicine applications and the treatment of degenerative diseases.Type: GrantFiled: March 1, 2013Date of Patent: October 23, 2018Assignee: UNIVERSITY OF MIAMIInventors: Herman S. Cheung, Daniel Pelaez, C-Y Charles Huang
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Patent number: 10064893Abstract: Compositions and methods are provided for the treatment of cancer. An immune effector cell population is pre-infected with an oncolytic virus. The combined therapeutic is safe and highly effective, producing an enhanced anti-tumor effect compared to either therapy alone. The methods of the invention thus provide for a synergistic effect based on the combined biotherapeutics.Type: GrantFiled: July 6, 2015Date of Patent: September 4, 2018Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Christopher H. Contag, Stephen H. Thorne
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Patent number: 10047341Abstract: Provided herein are methods for the generation of functional keratinocyte stem cells that are differentiated directly from human ESCs/iPSCs in a chemically defined serum-free cell culture system, as well as cells derived therefrom and methods of use thereof. Also provided are methods for culturing primary keratinocytes.Type: GrantFiled: October 13, 2015Date of Patent: August 14, 2018Assignee: FUJIFILM Cellular Dynamics, Inc.Inventors: Junying Yu, Ying Zhang, Lisa Harms
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Patent number: 10035118Abstract: A system and method for isolating and separating lipoaspirate particles. The system includes a generally cylindrical container having a lid and a bottom wherein the container includes at least one input port positioned to permit a lipoaspirate fluid to enter the container above the bottom; and a source of a vacuum coupled to the container to provide a partial vacuum during use of the system.Type: GrantFiled: April 30, 2012Date of Patent: July 31, 2018Assignee: SpineSmith Partners, L.P.Inventors: Ted Sand, David Chau, Matthew R. DeWitt, Sujata Ghosh, Kevin Hao-Yu Lin
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Patent number: 10030057Abstract: Described herein are novel compositions comprising multimodal TRAIL agents and cells engineered to express such multimodal TRAIL agents, including cells encapsulated in a scaffold or matrix, for use in the treatment of disorders such as cancer.Type: GrantFiled: August 1, 2016Date of Patent: July 24, 2018Assignee: THE GENERAL HOSPITAL CORPORATIONInventor: Khalid Shah
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Patent number: 10018040Abstract: A technique facilitates detection and analysis of constituents, e.g. chemicals, which may be found in formation fluids and/or other types of fluids. The technique comprises intermittently introducing a first fluid and a second fluid into a channel in a manner which forms slugs of the first fluid separated by the second fluid. The intermittent fluids are flowed through the channel to create a mixing action which mixes the fluid in the slugs. The mixing increases the exchange, e.g. transfer, of the chemical constituent between the second fluid and the first fluid. The exchange aids in sensing an amount of the chemical or chemicals for analysis. In many applications, the intermittent introduction, mixing, and measuring can be performed in a subterranean environment.Type: GrantFiled: October 25, 2015Date of Patent: July 10, 2018Assignee: SCHLUMBERGER TECHNOLOGY CORPORATIONInventors: Farshid Mostowfi, Ronald E. G. van Hal, Shahnawaz Hossain Molla, Jane T. Lam, Amy Du, Neil William Bostrom, Michael Mallari Toribio
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Patent number: 10018630Abstract: An objective of the present invention is to provide: a cancer stem cell isolated using a cell marker; a substantively homogeneous cancer stem cell population including said cancer stem cell; and a method of preparing said cancer stem cell population. Another objective of the present invention is to provide: a method for separating cancer stem cells with a high proliferative potential and those with a low proliferative potential; a method for inducing cancer stem cells to have a different proliferative potential; and cancer stem cells separated or induced by these separation or induction methods. A further objective of the present invention is to provide: a method of screening for pharmaceuticals using said cancer stem cell or cancer stem cell population; a method for detecting the presence of said cancer stem cell or cancer stem cell population and for identifying or quantifying the same.Type: GrantFiled: September 7, 2012Date of Patent: July 10, 2018Assignees: CHUGAI SEIYAKU KABUSHIKI KAISHA, PHARMALOGICALS RESEARCH PTE. LTD.Inventors: Tatsumi Yamazaki, Hisafumi Okabe, Shinta Kobayashi, Takeshi Watanabe, Koichi Matsubara, Atsuhiko Kato, Masami Suzuki
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Patent number: 10010883Abstract: A deterministic lateral displacement array that includes a channel, within a substrate, having a first sidewall, a second sidewall, and a channel length. A condenser portion that includes an entry port and an exit port. A first array of pillars is disposed between the entry port and the exit port of the condenser portion along the channel length, the first array of pillars operative to drive a first material particle and a second material particle towards the first sidewall of the channel. A separator portion that includes an entry port and an exit port, and a second array of pillars disposed between the entry port and the exit port of the separator portion along the channel length, the pillars operative to drive the first material particle towards the second sidewall of the channel.Type: GrantFiled: September 20, 2016Date of Patent: July 3, 2018Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Huan Hu, Sung Cheol Kim, Joshua T. Smith, Benjamin H. Wunsch
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Patent number: 10000740Abstract: This invention relates to the differentiation of pluripotent cells (PSCs) into foregut stem cells (FSCs) using a definitive endoderm induction medium comprising a TGFfi ligand, fibroblast growth factor (FGF), bone morphogenetic protein (BMP) and a PI3K inhibitor to differentiate the pluripotent cells into definitive endoderm cells and a foregut induction medium comprising a TGF? ligand to differentiate the definitive endoderm cells into foregut stem cells (FSCs). Methods of differentiation, populations of foregut stem cells, culture media and kits are provided.Type: GrantFiled: October 6, 2014Date of Patent: June 19, 2018Assignee: Cambridge Enterprise LimitedInventors: Ludovic Vallier, Nicholas Hannan
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Patent number: 10000813Abstract: The invention relates to method and compositions for treating and diagnosing cancer, in particular ?-catenin related cancers. In general, the methods include administering RNAi constructs. The invention further relates to methods of identifying CTNNB1 related cancer therapeutics.Type: GrantFiled: January 3, 2012Date of Patent: June 19, 2018Assignee: The United States of America, as represented by the Secretary, Department of Health & Human ServicesInventors: Mark J. Roth, Konrad Huppi
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Patent number: 9994810Abstract: The present invention relates to an a process for manufacturing dry powder cell culture media. The preparation and usage of mixed particles generated by co-lyophilisation leads to homogenously blended cell culture media.Type: GrantFiled: May 17, 2013Date of Patent: June 12, 2018Assignee: MERCK PATENT GMBHInventor: Joerg Von Hagen
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Patent number: 9994812Abstract: The present invention generally relates to the field of cell growth and tissue engineering, in particular, tissue engineered compositions comprising a nanotextured substrate which is structurally configured for growth of cells in an anatomically correct adult phenotype in vitro. In particular, described herein are nanotextured substrates which are structurally configured for the anisotropic organization, maturation, and growth of in vitro-differentiated muscle cells, such as cardiomyocytes, and methods for the production and use thereof in varying sizes, nanotextures and substrate rigidities. In vitro-differentiated cardiomyocytes grown on the nanotextured substrates described herein are better-differentiated and more closely mimic adult cardiac tissue than the same cells grown on a non-textured substrate of the same composition.Type: GrantFiled: March 15, 2013Date of Patent: June 12, 2018Assignee: University of Washington through Its Center For CommercializationInventors: Deok-Ho Kim, Michael Laflamme, Charles Murry, Kshitiz Gupta, Hyok Yoo, Alex Jiao
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Patent number: 9994822Abstract: This invention provides a method for testing a factor for cardiogenicity which comprises differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence and absence of the factor wherein the human embryonic stem (hES) cells are cultured under a serum free condition comprising co-culture in the presence of END-2 cells or serum-free extracellular medium therefrom, and measuring the differentiation in the presence and absence of the factor. This invention also provides a method for identifying a cardiogenic factor, which comprises differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence or absence of the factor wherein the human embryonic stem (hES) cells are cultured under a serum free condition comprising co-culture in the presence of END-2 cells or serum-free extracellular medium therefrom, and identifying the factor that affects the differentiation of human embryonic stem (hES) cells to cardiomyocytes in the presence or absence of the factor.Type: GrantFiled: August 24, 2015Date of Patent: June 12, 2018Assignee: ES Cell International Pte LtdInventors: Robert Passier, Christine L. Mummery
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Patent number: 9994849Abstract: Provided is a method of separating senescent cells from a sample including the senescent cells, and a method of removing senescent cells from a sample or a subject including the senescent cells.Type: GrantFiled: December 21, 2016Date of Patent: June 12, 2018Assignee: SAMSUNG ELECTRONICS CO., LTD.Inventors: Byungju Kim, Sungjin Ryu, Sangchul Park
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Patent number: 9988606Abstract: Embodiments of the present invention are directed to various methods for generating airway and lung progenitors and epithelial cells and three-dimensional anterior foregut spheres, and to populations of cells made using the methods. The airway and lung progenitors and epithelial cells can be used as a model to study diseases that primarily affect airway epithelial cells, and to study human lung development. Methods are also provided for drug screening. Anterior foregut spheres can be used as a model for lung fibrosis.Type: GrantFiled: July 24, 2013Date of Patent: June 5, 2018Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORKInventors: Hans-Willem Snoeck, Sarah Xuelian Huang, Ya-Wen Chen, Jahar Bhattacharya, Mohammad Naeem Islam
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Patent number: 9988605Abstract: The present invention prepared insulin-producing endocrine cells by inducing the differentiation of human embryonic stem cells or human induced pluripotent stem cells into definitive endoderm (DE), pancreatic endoderm (PE), endocrine progenitors (EP), and endocrine cells (EC) stepwise in that order. Particularly, the present invention established the conditions for the formation of an insulin producing endocrine aggregate (EA) from the endocrine cells. Especially in this invention, it was confirmed that the endocrine aggregate has the cell proliferation potential at a significant level and has the increased insulin productivity as well as the activity of inhibiting cell necrosis and apoptosis. Therefore, the method for preparing the endocrine aggregate of insulin-producing beta cells from human pluripotent stem cells can be effectively used for the examination of the medicinal effect of the conventional antidiabetic agents and of the novel antidiabetic drugs.Type: GrantFiled: August 7, 2014Date of Patent: June 5, 2018Assignee: Korea Advanced Institute of Science and TechnologyInventors: Yong-mahn Han, Young-jin Kim, Hail Kim
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Patent number: 9982233Abstract: The present invention relates to a method of promoting generation of exosomes from stem cell by using thrombin. The method according to the present invention has superior effects of promoting generation of stem cell-derived exosomes and thus exosomes can be more efficiently obtained thereby compared to conventionally known methods. In addition, the method can be useful for related research.Type: GrantFiled: December 12, 2014Date of Patent: May 29, 2018Assignee: SAMSUNG LIFE PUBLIC WELFARE FOUNDATIONInventors: Yun Sil Chang, Won Soon Park, Dong Kyung Sung, So Yoon Ahn
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Patent number: 9982231Abstract: Compositions and methods are described herein for chemically inducing cells to change their differentiation state and become cardiac progenitor cells or cardiomyocytes.Type: GrantFiled: March 10, 2016Date of Patent: May 29, 2018Assignee: THE J. DAVID GLADSTONE INSTITUTES, A TESTAMENTARY TRUST ESTABLISHED UNDER THE WILL OF J. DAVID GLADSTONEInventors: Nan Cao, Sheng Ding
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Patent number: 9969982Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells and the products related to or resulting from such methods. In particular, the present invention provides an improved method for the formation of pancreatic hormone expressing cells and pancreatic hormone secreting cells. In addition, the present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer and the products related to or resulting from such methods. The present invention also provides methods to promote glucose-stimulated insulin secretion in insulin-producing cells derived from pluripotent stem cells.Type: GrantFiled: May 21, 2015Date of Patent: May 15, 2018Assignee: LifeScan, Inc.Inventor: Alireza Rezania
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Patent number: 9963679Abstract: Methods of preparing pancreatic islets that may comprise pancreatites are provided. The methods may involve subjecting a pancreas and/or pieces thereof to disruption, such as, for example, an enzyme digest, and seeding the recovered cellular product comprising islets in a culture medium comprising at least a detectable amount of endocrine tissue and/or exocrine tissue.Type: GrantFiled: October 21, 2011Date of Patent: May 8, 2018Assignee: LIFELINE SCIENTIFIC, INC.Inventors: Michael J. Taylor, Simona C. Baicu
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Patent number: 9950095Abstract: The invention provides a method for producing an implant from interstitial, connective or supporting tissue, the method comprising at least one step of perfusing the tissue with at least one decellularization medium under negative pressure applied for substantially the whole time period of the perfusion.Type: GrantFiled: June 16, 2014Date of Patent: April 24, 2018Assignee: Videregen LimitedInventors: Tahera Iqbal Ansari, Paul David Sibbons
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Patent number: 9944901Abstract: Provided is a protein coded by a gene related to cell totipotency and a transcriptional activation domain of a mammalian YAP protein or a fusion protein of a segment with a transcriptional control activity, a coding nucleotide sequence, an expression vector and a composition thereof, as well as a method for inducing the pluripotent stem cells by using the fusion protein.Type: GrantFiled: June 17, 2014Date of Patent: April 17, 2018Assignees: Institute of Zoology, Chinese Academy of Sciences, Emory University School of MedicineInventors: Dahua Chen, Peng Jin, Qinmiao Sun, Weiqi Tan
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Patent number: 9938500Abstract: Methods of generating and expanding human hemangio-colony forming cells in vitro and methods of expanding and using such cells are disclosed. The methods permit the production of large numbers of hemangio-colony forming cells as well as derivative cells, such as hematopoietic and endothelial cells. The cells obtained by the methods disclosed may be used for a variety of research, clinical, and therapeutic applications.Type: GrantFiled: September 29, 2015Date of Patent: April 10, 2018Assignee: Astellas Institute for Regenerative MedicineInventors: Robert Lanza, Shi-Jiang Lu
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Patent number: 9932556Abstract: The present invention relates to a method for in vitro differentiation of a population of blood circulating cells, such as monocytes and preferably pluripotent macrophages derived therefrom, into cells displaying functional and phenotypic neuronal characteristics. The invention further encompasses neuronal-like cells obtainable according to the present method, compositions comprising said cells, and applications thereof.Type: GrantFiled: October 16, 2014Date of Patent: April 3, 2018Assignees: Centre National de la Recherche Scientifique (CNRS), Universite Paris Descartes, Institut Ntaitonal de la Sante et de la recherche Medicale (INSERM), Assistance Publique—Hopitaux de ParisInventors: Anne Hosmalin, Vincent Feuillet, Marie-Odile Krebs, Thérèse Jay, Alfredo Bellon
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Patent number: 9920299Abstract: Methods are disclosed for generating HLA homozygous parthenogenetic human stem cell (hpSC-Hhom) lines from both HLA homozygous and HLA heterozygous donors. These hpSC-Hhom lines demonstrate typical human embryonic stem cell morphology, expressing appropriate stem cell markers and possessing high levels of alkaline phosphatase and telomerase activity. Additionally, injection of these cell lines into immunodeficient animals leads to teratoma formation. Furthermore, in the case of HLA heterozygous donors, the hpSC-Hhom lines inherit the haplotype from only one of the donor's parents. SNP data analysis suggests that hpSC-Hhom lines derived from HLA heterozygous oocyte donors are homozygous throughout the genome as assessed by single-nucleotide polymorphism (SNP) analysis. The protocol as disclosed minimizes the use of animal-derived components, which makes the stem cells more practical for clinical application.Type: GrantFiled: November 24, 2015Date of Patent: March 20, 2018Assignee: International Stem Cell CorporationInventors: Elena S. Revazova, Leonid N. Kuzmichev, Nickolay A. Turovets, Jeffrey D. Janus
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Patent number: 9903796Abstract: A photocleavable polymer comprising a photocleavable linker and a polymer having a functional group that binds to a protein, lipid, sugar of a cell, or any combination thereof, as well as a method of selectively lysing cells by incubating a sample comprising two or more cells with the photocleavable polymer to reversibly fix the cells; selectively irradiating the fixed cells or a target or non-target cell among the fixed cells to cleave the photocleavable polymer; and adding a cell lysis solution to selectively lyse the irradiated cells.Type: GrantFiled: July 3, 2014Date of Patent: February 27, 2018Assignee: SAMSUNG ELECTRONICS CO., LTD.Inventors: Hun-joo Lee, Yeon-jeong Kim, Dong-hyun Park, Jong-myeon Park
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Patent number: 9884076Abstract: The present disclosure relates generally to methods for the increased processing of tissue for the generation of cardiac stem cells, wherein the stem cells are suitable for use in cardiac stem cell therapy. In particular, several embodiments relate to the processing of allogeneic donor cardiac tissue for the generation of multiple patient doses of cardiac stem cells.Type: GrantFiled: May 31, 2013Date of Patent: February 6, 2018Assignee: Capricor, Inc.Inventors: Michelle Kreke, Rachel Smith
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Patent number: 9868936Abstract: The present invention is directed towards methods for producing cell and tissue compositions suitable for therapeutic applications to a mammal in need of a therapeutic cell or tissue treatment. In particular, the invention is directed towards a method of culturing cells for use in therapy, which method comprises (i) culturing a sample of cells in a first cell culture medium and (ii) prior to harvesting cells for use in therapy culturing the cells in a second cell culture medium.Type: GrantFiled: November 3, 2014Date of Patent: January 16, 2018Assignee: ISOPOGEN PTY LTDInventor: Marian June Sturm
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Patent number: 9856293Abstract: A synthetic peptide having nestin expression inducing ability which allows induction of expression of nestin in astrocytes; an agent for inducing nestin expression including the peptide as an active ingredient; and a method for producing high nestin expressing cells derived from astrocytes, the method being characterized in that the agent for inducing nestin expression is supplied. The agent for inducing nestin expression provided by the present invention includes, as an active ingredient, a synthetic peptide including a nestin-inducing peptide sequence consisting of an amino acid sequence of SEQ ID NO: 1 or an amino acid sequence formed by conservative replacement of 1, 2 or 3 amino acid residues in the amino acid sequence. The method for producing high nestin expressing cells derived from astrocytes provided by the present invention includes preparing an astrocyte culture, and supplying, at least once, the agent for inducing nestin expression to the astrocyte culture.Type: GrantFiled: October 10, 2014Date of Patent: January 2, 2018Assignee: TOAGOSEI CO. LTD.Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida
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Patent number: 9844514Abstract: This application discloses methods for treating an established myocardial infarction, including treatment with an epicardial construct containing stromal vascular fraction (SVF) from adipose tissue which may be seeded onto a biocompatible substrate, which preserves microvascular function and LV contractile mechanisms.Type: GrantFiled: July 9, 2014Date of Patent: December 19, 2017Assignee: University of Louisville Research Foundation, Inc.Inventors: Stuart K. Williams, James B. Hoying, Amanda J. LeBlanc
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Patent number: 9845460Abstract: The present invention is provides a method for treating human pluripotent cells. In particular, the methods of the invention are directed to the treatment of human pluripotent cells, whereby the human pluripotent cells can be efficiently expanded in culture and differentiated by treating the pluripotent cells with an inhibitor of glycogen synthase kinase 3? (GSK-3B) enzyme activity.Type: GrantFiled: November 20, 2013Date of Patent: December 19, 2017Assignee: Janssen Biotech, Inc.Inventors: Janet Davis, Jiajian Liu
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Patent number: 9822343Abstract: This document provides methods and materials involved obtaining induced pluripotent stem (iPS) cells. For example, methods and materials for increasing the efficiency for making iPS cells as well as methods and materials for selecting iPS cells are provided.Type: GrantFiled: August 21, 2012Date of Patent: November 21, 2017Assignee: Mayo Foundation for Medical Education and ResearchInventors: Timothy J. Nelson, Andre Terzic, Almudena J. Martinez Fernandez, Clifford D. Folmes
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Patent number: 9790469Abstract: The subject matter of the invention is a functional population of human brown adipocytes, in which the expression of UCP1, CIDEA, CPT1B and Bc12 is higher, the expression of Bax is lower and the expression of PPAR-alpha, PGC-1alpha, PGC-1 beta and PRDM16 is similar compared with the corresponding expressions of a population of human white adipocytes. The invention also relates to a method for differentiation of hMADS cells into the functional population of human brown adipocytes, to a method for conversion of a population of human white adipocytes into the functional population of human brown adipocytes, and also to a method of screening for molecules capable of modulating the bodyweight in an individual.Type: GrantFiled: June 23, 2009Date of Patent: October 17, 2017Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), UNIVERSITE DE NICE SOPHIA-ANTIPOLISInventors: Gérard Paul Ailhaud, Ez-Zoubir Amri, Christian Jean Lucien Dani, Christian Elabd
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Patent number: 9783784Abstract: In some embodiments, methods for improving the survival of pancreatic ?-cell progenitors in culture are provided. Such methods may include contacting a population of pancreatic progenitor cells with an amino acid (aa) sequence comprising IKVAV (SEQ ID NO:1). In other embodiments, methods for (i) verifying the establishment of a population of pancreatic progenitor or stem cells and (ii) methods for generating or establishing a population of pancreatic endocrine progenitor cells in vitro are provided.Type: GrantFiled: March 14, 2013Date of Patent: October 10, 2017Assignees: CITY OF HOPE, CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Hsun Ku, David Tirrell