Abstract: The present invention relates to a method for detecting a quantitative trait locus (QTL) associated with resistance to Botrytis cinerea in tomato, comprising the steps of crossing a Botrytis-resistant donor tomato plant with a non-resistant, or Botrytis-susceptible, recipient tomato plant, contacting one or more offspring plants with an infective amount of Botrytis, quantitatively determining the disease incidence and/or the rate of lesion growth in said one or more offspring plants, establishing a genetic linkage map that links the observed disease incidence and/or rate of lesion growth to the presence of chromosomal markers of said donor tomato plant in said one or more offspring plants, and assigning to a QTL the contiguous markers on said map that are linked to a reduced disease incidence and/or a reduced lesion growth rate.

Abstract: The invention provides seed and plants of the tomato variety designated EX01419137. The invention thus relates to the plants, seeds and tissue cultures of tomato variety EX01419137 and to methods for producing a tomato plant produced by crossing a plant of tomato variety EX01419137 with itself or with another tomato plant, such as a plant of another variety. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of tomato variety EX01419137 including the fruit and gametes of such plants. The invention also relates to tomato variety CHI 14-2079. The present invention is also directed to tomato variety CHD 14-2080.

Abstract: The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

Abstract: Compositions and methods for providing nematode resistance are provided. One aspect provides transgenic plants or cells comprising an inhibitory nucleic acid specific for one or more nematode esophageal polypeptides. Other aspects provide transgenic plants or cells resistant to at least two different root-knot nematode species.

Abstract: The present invention provides isolated polynucleotide sequences encoding ET37, TIC810 and TIC812 proteins from Bacillus thuringiensis, and nucleotide sequences for use in expressing TIC809, ET37, TIC810 and TIC812, and fusions of various insecticidally effective combinations of these proteins such as TIC 127, in plants. Methods of making and using the polynucleotide sequences and the proteins in the development of transgenic plant cells and transgenic plants exhibiting improved insect resistance against (1) Coleopteran insects including Western Corn Rootworm (Dibrotica virgifera), Southern Corn Rootworm (Dibrotica undecempunctata), Northern Corn Rootworm (Diabrotica barbed), Mexican Corn Rootworm (Diabrotica virgifera zeae), Brazilian Corn Rootworm (Diabrotica balteata) and Brazilian Corn Rootworm complex (Diabrotica viridula and Diabrotica speciosa), and against Hemipteran insects such as Lygus bugs, are disclosed.

Abstract: The present invention relates to a nucleic acid construct having a nucleic acid molecule configured to silence ?-carotene hydroxylase expression, and host cells, expression systems, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of enhancing beta-carotene content by growing a transgenic plant from a plant or seed transformed with the construct.

Abstract: The present invention relates to an isolated polynucleotide capable of giving a plant tolerance to drought and/or salt stress, which comprises a polynucleotide sequence as shown in SEQ ID NO:1, and to a promoter capable of giving a plant tolerance to drought and/or salt stress. The present invention also relates to an expression vector comprising the said polynucleotide and/or the said promoter, and to a host cell transformed or transfected by the said expression vector. The present invention further relates to a use of the said polynucleotide or promoter sequence in improvement of plant tolerance to drought and/or salt stress.

Abstract: The present invention provides non-coding regulatory element polynucleotide molecules isolated from the ribosomal protein L18A and heat shock cognate HSC71 genes of Oryza sativa and useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Oryza sativa regulatory polynucleotide sequences, and methods for preparing and using the same.

Abstract: Methods for identifying one or more amino acid substitutions in a Rubisco large subunit polypeptide (variant) that confer increased Rubisco activity in a unicellular photosynthetic organism and transferring those substitutions to a Rubisco large subunit polypeptide of a higher plant cell are described herein. Methods and compositions for modulating plant productivity using the modified Rubisco large subunit polypeptide variants are provided. The Rubisco large subunit sequences are used in a variety of methods including increasing plant productivity in a plant. Transformed plants, plant cell, tissues, seed, and expression vectors are also provided.

Abstract: A nucleic acid containing a glutelin gene promoter. Disclosed are transformed plant cells and transgenic plants containing a nucleic acid that includes the promoter operably linked to a sequence encoding heterologous protein. Also disclosed are methods of making the transformed plant cells and transgenic plants and methods for expressing a polypeptide.

Abstract: Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novel B. thuringiensis genes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Abstract: The present invention relates to a transformed plant cell expressing ?-amyloid gene, which is known as a causal substance of senile dementia in human, a transformed plant and a preparation method of the same, more particularly, to a transformed plant cell expressing tandem repeats of ?-amyloid gene, a transformed plant produced by tissue-culturing the above cells and a preparation method of the same. The transformed plant of the present invention expresses multiple ?-amyloid protein, so that the plant can be effectively used for the preparation of an oral vaccine composition for the prevention of senile dementia in human.

Abstract: Transformed plants which have increased or decreased linolenic acid content are disclosed. Also disclosed are plants which express a linoleic acid desaturase gene.

Abstract: The present invention relates to new DNA sequences encoding an insecticidal Cry9Fa protein and insecticidal parts thereof, which are useful to protect plants from insect damage. Also included herein are micro-organisms and plants transformed with a DNA sequence encoding an insecticidal Cry9Fa protein and processes for controlling insects and to obtain a plant resistant to insects.

Abstract: The present invention provides non-coding regulatory element polynucleotide molecules isolated from Oryza sativa and useful for expressing transgenes in plants, in particular root tissues. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Oryza sativa regulatory polynucleotide sequences, and methods for preparing and using the same.

Abstract: The present invention relates to a rapid and large-scale production of tomato mutants by utilizing a miniature tomato plant which can be crossed with tomato commercial plants. Mutations are induced in the miniature tomato cultivars and desired mutants are subsequently identified in the resulting mutant tomato population.

Abstract: The inventions is drawn towards vectors and methods useful for preparing genetically transformed plant cells that express immunogens from pathogenic organisms which are used to produce immunoprotective particles useful in vaccine preparations. The invention includes plant optimized genes that encode the HN protein of Newcastle Disease Virus. The invention also relates to methods of producing an antigen in a transgenic plant.

Abstract: The use of DNA sequences comprising a glucanase coding region operably linked to a promoter, or other regulatory sequence, which provides for expression of the DNA sequence with appropriate tissue and/or temporal specificity, in the preparation of a male sterile transgenic tomato plant is disclosed. In preferred embodiments the promoter is a tapetum specific promoter, eg an A3 or an A9 promoter. DNA sequences comprising the PR-Glucanase coding region and an A3 or an A9 promoter, preferably an A9 promoter are also described, as are transgenic tomato plants, plant cells, propagating material, seeds, antisense DNA sequences and ribozyme encoding DNA sequences for restoration of male-fertility.

Abstract: The subject invention pertains to materials and methods for producing plants that are resistant to infection by geminiviruses and other related viruses. Methods of the invention comprise transforming a plant with a polynucleotide wherein when the polynucleotide is expressed in the plant, the transformed plant exhibits resistance to plant viral infections. Exemplified herein is the use of a polynucleotide encoding a Rep protein derived from tomato mottle geminivirus. The methods of the invention can be used to provide resistance to viral infection in plants such as tomato and tobacco. The present invention also concerns transformed and transgenic plants in plant tissue that express a polynucleotide encoding a plant virus Rep protein, or a fragment or variant thereof.

Abstract: The invention provides a purified or recombinant phytase enzyme (SEQ ID NO:2) initially derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.

Abstract: The present invention provides for the nucleic acid sequences of plant centromeres. This will permit construction of stably inherited recombinant DNA constructs and minichromosomes which can serve as vectors for the construction of transgenic plant and animal cells.

Abstract: A method for obtaining a transgenic plant that over-expresses a soluble isoform AGPPase enzyme. The method includes a step of transforming a plant with a vector comprising a polynucleotide of SEQ ID NO: 7 linked to a promoter that promotes expression of the polynucleotide in the plant whereby to form the transgenic plant. The transgenic plant has a reduced starch content and a higher resistance to salinity than the plant before the transforming step.

Abstract: This invention relates to a tomato plant, seed, variety and hybrid. More specifically, the invention relates to a tomato plant having a mutant allele for allflesh which results in the tomato fruit having a cavity area which is solid and lacks a gel or liquid. The invention also relates to crossing inbreds, varieties and hybrids containing the allflesh allele to produce novel types and varieties of tomato plants.

Abstract: A novel CrtZ carotenoid hydroxylase, isolated from Brevundimonas vesicularis DC263, is provided that is useful for the production of hydroxylated carotenoids. Additionally, a previously identified hypothetical protein from Novosphingobium aromaticivorans has found to have carotenoid hydroxylase activity. Both hydroxylase genes exhibit low homology in comparison to other CrtZ hydroxylases previously reported. Expression of the hydroxylases in heterologous host cells enabled production of hydroxylated carotenoids.

Abstract: An inbred maize line, designated PH705, the plants and seeds of inbred maize line PH705, methods for producing a maize plant, either inbred or hybrid, produced by crossing the inbred maize line PH705 with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred line PH705 with another maize line or plant and to methods for producing a maize plant containing in its genetic material one or more transgenes and to the transgenic maize plants produced by that method. This invention also relates to inbred maize lines derived from inbred maize line PH705, to methods for producing other inbred maize lines derived from inbred maize line PH705 and to the inbred maize lines derived by the use of those methods.

Abstract: The present invention relates to a method for producing recombinant rotavirus structural proteins by culturing a transformed plant cell, and to a vaccine composition comprising the same as an effective component. More specifically, the method comprises the steps of preparing the expression vector comprising cDNA fragments encoding rotavirus structural proteins; transforming a plant cell with the expression vector; and recovering the vaccine composition, including rotavirus antigens, from the cell culture. The method for producing the recombinant rotavirus structural proteins is advantageous in that higher yields of more than 0.3 mg/L are obtained, as well as a lower cost and a lower contamination hazard than those of eukaryotic expression systems such as baculovirus and animal cell. Furthermore, the method can be used to produce a edible vaccine. The invention also proves that the rotavirus structural protein can be produced with a high aspect rotary vessel reactor.

Abstract: An inbred tomato line, designated 294, is disclosed. The invention relates to the seeds of inbred tomato line 294, to the plants of inbred tomato line 294 and to methods for producing a tomato plant, either inbred or hybrid, by crossing the inbred line 294 with itself or another tomato line. The invention further relates to methods for producing a tomato plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred tomato lines derived from the inbred 294.

Abstract: The invention relates to a distinct tomato line, designated the Goose Creek tomato line. Additionally, the invention relates to seed and fruit of the Goose Creek tomato line as well as methods of producing the plant, fruit, and seed of the Goose Creek tomato line. The invention also relates to Goose Creek-derived tomato plants, fruit, and seed and methods of producing the Goose Creek-derived plants, fruit, and seed.

Abstract: The current invention provides nucleic acid sequences encoding the RIN and MC genes. Compositions comprising these sequences are described, as are plants transformed with such compositions. Further provided are methods for the expression of the RIN and MC genes. The methods of the invention include the direct creation of transgenic plants with the RIN and MC genes by genetic transformnation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: The present invention relates to a rapid and large-scale production of tomato mutants by utilizing miniature tomato plants which can be crossed with tomato commercial plants. Mutations are induced in the miniature tomato cultivars and desired mutants are subsequently identified in the resulting mutant tomato population.

Abstract: The invention relates to the nucleotide sequence from the sugarcane ubi9 polyubiquitin gene promoter, which is capable of directing constitutive expression of a nucleic acid sequence of interest that is operably linked to it. The sugarcane ubi9 promoter is useful in regulating expression of a nucleic acid sequence of interest in monocotyledonous and dicotyledonous plants.

Abstract: A method for modifying structural gene sequences to enhance the expression of the protein product is disclosed. Also disclosed are novel structural genes which encode insecticidal proteins of B.t.k. HD-1, B.t.k. HD-73, B.t. tenebrionis, B.t. entomocidus, 2 protein of B.t.k. HD-1, and the coat protein of potato leaf roll virus.

Abstract: The invention involves recombinant, double-stranded DNA that contains a promoter which functions in plant cells to cause the production of RNA sequences of a plant virus, a DNA sequence that causes the production of an RNA sequence encoding the coat protein of said plant virus, and a 3′ non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3′ end of said RNA sequence; which double-stranded DNA can be used in a method for genetically transforming plants to produce genetically transformed plant cells and plants that are resistant to virus infection.

Abstract: This invention relates to plant cells, plant tissues or plants transgenic for a nucleic acid encoding a puroindoline. This invention also relates to methods of producing such transgenic plant cells, plant tissues or plants. The transgenic plants produced by the methods of this invention are useful in reducing the damage caused by plant pests, especially plant pathogens such as fungi and bacteria.

Abstract: The present invention provides yellow tomato plants producing fruit suitable for processing.

Abstract: The present invention is directed to isolated fragments of an Erwinia hypersensitive response elicitor protein or polypeptide which fragments elicit a hypersensitive response in plants. Also disclosed are isolated DNA molecules which encode the Erwinia hypersensitive response eliciting fragment. Isolated fragments of hypersensitive response elicitor proteins or polypeptides, which elicit a hypersensitive response, and the isolated DNA molecules that encode them can used to impart disease resistance to plants, to enhance plant growth, and/or to control insects on plants. This can be achieved by applying the hypersensitive response eliciting fragments in a non-infectious form to plants or plant seeds under conditions effective to impart disease resistance, to enhance plant growth, and/or to control insects on plants or plants grown from the plant seeds.

Abstract: This application discloses the tissue-specific transcription regulatory sequences from the soybean Msg, gene, which is highly expressed in tissues which are potential sites of entry of plant pathogens or attack by plant pests, including the developing soybean pods. The Msg gene shows significant homology to a family of fruit and flower specific genes, designated the major latex protein (MLP) homologs, so far reported in only a few species and whose functions are unknown. The Msg transcription regulatory sequences are fully active in Arabidopsis only in plants transformed with the 2.26 kb fragment promoter, expressing an associated coding sequence in nectaries, nodes, short style and in guard cells of the silique, pedicel and stem but not in mature leaves. Methods for tissue specific sequence expression are provided in the present application.

Abstract: The invention concerns the use of recombinant nucleotides sequences containing cDNA coding for a preduodenal lipase, or any sequence derived from this cDNA, for transforming plant cells in order to obtain recombinant preduodenal lipase or polypeptide derivatives. The invention also concerns the use of genetically modified plants or parts thereof, or extracts of these plants or the use of recombinant preduodenal lipase or résultant polypeptide derivatives in the field of foodstuffs, or for producing medicaments, or in industry.

Abstract: Transformed plant cells which have increased'starch content are disclosed. Also disclosed are whole plants comprising plant cells which express CTP/ADPglucose pyrophosphorylase genes.

Abstract: Introducing sucrose phosphorylase activity into plants by transformation with a gene for the enzyme increases the rate of sucrose hydrolysis, leading to increased starch, oil, and protein levels. The preferred gene is from Streptococcus mutans. Surprisingly, in potatoes transformed to express this gene in tubers, reduced bruise discoloration susceptibility and increased uniformity of starch deposition throughout the tuber are achieved.

Abstract: The invention relates in general to plants, plant cells, methods of making, and methods of using plants and plant cells transformed to contain a DNA sequence encoding an AMPA-N-acetyltransferase, and to plants and plant cells exhibiting resistance to AMPA in an amount which inhibits the growth of a plant or plant cell lacking a sequence encoding an AMPA-N-acetyltransferase.

Abstract: The present invention relates to the isolation of two DNA promoters from a coffee plant. The isolated promoters, one inducible and one constitutive, are capable of inducing the expression of a second DNA operably linked to the promoter. The present invention also relates to host cells, expression systems and transgenic plants containing the promoters of the invention.

Abstract: Nucleic acid molecules that encode a plant promoter involved in photoperiodism and circadian rhythms are disclosed. These molecules may be introduced into plants in order to alter the photoperiodic and/or circadian clock-based gene expression of the plants.

Abstract: The present invention relates to the DNA sequence for eukaryotic genes encoding &egr; cyclase isolated from romaine lettuce as well as vectors containing the same and hosts transformed with said vectors. The present invention provides methods for controlling the ratio of various carotenoids in a host and to the production of novel carotenoid pigments. The present invention also provides a method for treating disease by administering carotenoids obtained from transformed hosts, or by administering a composition containing the transformed hosts.

Abstract: A gene amplification system based on plant viral genetic elements dramatically increases foreign protein production in plants. A safer and more economical production system for vaccines and antibodies in recombinant plants grown using agricultural practice is described. The high-level expression system uses the replicative process of a plant mastrevirus, exemplified by bean yellow dwarf virus (BeYDV). The expression system is preferably inducible to avoid interference with plant growth and development. Developmental cues, such as fruit ripening, are employed to trigger expression of the foreign protein using a tissue-specific promoter. A single, stably integrated expression cassette for foreign protein is replicated extrachromosomally in ripening fruit, forming hundreds of transcriptionally competent copies. Preferred plant hosts include tomato as a model system and soybean for production of large quantities of protein at high total protein levels.

Abstract: The present invention relates generally to a transgenic fruit-bearing plant having a foreign nucleotide sequence inserted into its genome which is substantially similar to a portion of the plant's fruit ripening specific lipoxygenase cDNA. Transgenic plants according to the present invention produce fruits having modified and surprisingly superior ripening characteristics, including improved quality and texture, greater firmness, longer shelf life, better packaging and storage characteristics, and improved processing characteristics. Also provided are transgenic fruits; transgenic plant cells; methods for making transgenic plants, fruits and plant cells; methods for inhibiting lipoxygenase production in plants; isolated nulceic acid sequences; and vectors comprising these isolated nucleotide sequences.

Abstract: The invention provides the identification and characterization of plant SNF4 and SNF1 genes. Examples of the genes were cloned from tomato. They are nucleic acids and proteins belonging to the SNF1-related protein kinase family, and are involved in plant's response to stress conditions such as nutritional and environmental stresses. The invention also provides transgenic plants containing the genes, and methods of modulating stress responses in transgenic plants comprising these genes.

Abstract: A method is provided for expressing a mammalian antigen in transformed plants to provide a source of plant material for oral or enteral administration to a mammal to produce tolerance to the antigen.

Abstract: Introducing sucrose phosphorylase activity into plants by transformation with a gene for the enzyme increases the rate of sucrose hydrolysis, leading to increased starch, oil, and/protein levels. Sucrose phosphorylase genes from Streptococcus mutans and Leuconostoc mesenteroides have been found particularly advantageous for use in the present invention. Surprisingly, in potatoes transformed to express these genes in tubers, reduced bruise discoloration susceptibility and increased uniformity of starch deposition throughout the tuber are achieved.

Abstract: Sucrose phosphate synthase (SPS), its process for preparation, its cDNA, and utilization of cDNA to modify the expression of SPS in the plant cells are provided.