Abstract: Compositions and methods for conferring hydroxyphenyl pyruvate dioxygenase (HPPD) herbicide resistance or tolerance to plants are provided. Compositions include amino acid sequences, and variants and fragments thereof, for mutant HPPD polypeptides. Nucleic acids that encode the mutant HPPD polypeptides are also provided. Methods for conferring herbicide resistance or tolerance, particularly resistance or tolerance to certain classes of herbicides that inhibit HPPD, in plants are further provided. Methods are also provided for selectively controlling weeds in a field at a crop locus and for the assay, characterization, identification and selection of the mutant HPPDs of the current invention that provide herbicide tolerance.

Abstract: A process to prepare a recombined transgenic Zea mays plant or plant cell from a first transgenic Zea mays plant cell, wherein the transgene in the recombinant plant or plant cell has an altered genetic structure relative to the genetic structure of the transgene in the first transgenic plant cell, due to homologous recombination-mediated transgene deletion.

Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract: Disclosed are methods of identifying peptides and compositions that bind to oocytes of animals in a species-specific manner. The methods utilize phage libraries and oocytes isolated from target species and non-target species.

Abstract: Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.

Abstract: Proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are polynucleotides capable of encoding these proteins, compositions that include one or more of these proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these compounds, diversification methods involving the compounds, and methods of using the compounds. Some of the methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract: The invention provides for identification and use of certain chloroplast transit peptides for efficient processing and localization of dicamba monooxygenase (DMO) enzyme in transgenic plants. Methods for producing dicamba tolerant plants, methods for controlling weed growth, and methods for producing food, feed, and other products are also provided, as well as seed that confers tolerance to dicamba when it is applied pre- or post-emergence.

Abstract: According to the invention, there is provided seed and plants of the hybrid corn variety designated CH889077. The invention thus relates to the plants, seeds and tissue cultures of the variety CH889077, and to methods for producing a corn plant produced by crossing a corn plant of variety CH889077 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH889077.

Abstract: According to the invention, there is provided seed and plants of the hybrid corn variety designated CH955017. The invention thus relates to the plants, seeds and tissue cultures of the variety CH955017, and to methods for producing a corn plant produced by crossing a corn plant of variety CH955017 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH955017.

Abstract: According to the invention, there is provided seed and plants of the hybrid corn variety designated CH457359. The invention thus relates to the plants, seeds and tissue cultures of the variety CH457359, and to methods for producing a corn plant produced by crossing a corn plant of variety CH457359 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH457359.

Abstract: The present invention provides novel promoters for use in plants. Specifically, the present invention provides novel chimeric promoters comprising combinations of plant viral enhancer elements and plant promoters. The present invention also provides DNA constructs; transgenic cells, plants, and seeds containing these novel chimeric promoters; and methods for preparing and using the same.

Abstract: Proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are polynucleotides capable of encoding these proteins, compositions that include one or more of these proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these compounds, diversification methods involving the compounds, and methods of using the compounds. Some of the methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract: Proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are polynucleotides capable of encoding these proteins, compositions that include one or more of these proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these compounds, diversification methods involving the compounds, and methods of using the compounds. Some of the methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract: The present invention relates, inter alia, a method of selectively controlling weeds at a locus comprising crop plants and weeds, wherein the method comprises application to the locus of a weed controlling amount of a pesticide composition comprising an homogentisate solanesyltransferase (HST) inhibiting herbicide and/or hydroxyphenyl pyruvate dioxygenase (HPPD) inhibiting herbicide, wherein the crop plants comprise at least one recombinant polynucleotide which comprises a region which encodes an HST; to a method of selectively controlling weeds at a locus comprising crop plants and weeds, wherein the method comprises application to the locus of a weed controlling amount of a pesticide composition comprising an homogentisate solanesyltransferase (HST) inhibiting herbicide, wherein the crop plants comprise at least one recombinant polynucleotide which comprises a region which encodes a HPPD enzyme and to recombinant polynucleotides and vectors for utilised in the methods.

Abstract: The invention provides transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products derived from event MON 87427. The invention also provides nucleotides specific for transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products comprising nucleotides specific for transgenic maize event MON 87427. The invention also provides methods related to transgenic maize event MON 87427 and to the Roundup® Hybridization System (RHS). The invention also provides a Relative Development Scale useful for monitoring and determining reproductive development in maize that reconciles developmental differences across various maize varieties. This is useful for determining the optimal timing of a treatment regimen in which tassel development stage is an important factor, including various methods in making hybrid seed.

Abstract: The invention provides for identification and use of certain chloroplast transit peptides for efficient processing and localization of dicamba monooxygenase (DMO) enzyme in transgenic plants. Methods for producing dicamba tolerant plants, methods for controlling weed growth, and methods for producing food, feed, and other products are also provided, as well as seed that confers tolerance to dicamba when it is applied pre- or post-emergence.

Abstract: The invention relates to a DNA vector comprising (a) a DNA sequence coding for the phytoene desaturase protein that is modified in one position by an amino add exchange providing resistance, and (b) a multiple cloning site into which any DNA sequence can be cloned. The invention also relates to the use of said DNA vector for transforming enkaryotic cells, transformation methods, and transgenic plant cells produced in said manner.

Abstract: The invention provides a modified variant of dicamba monooxygenase (DMO). The invention relates to the unexpected finding that cells expressing this DMO exhibit high levels of tolerance to the herbicide dicamba. Compositions comprising DMO-encoding nucleic acids and methods of use are provided.

Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract: The invention provides for identification and use of certain chloroplast transit peptides for efficient processing and localization of dicamba monooxygenase (DMO) enzyme in transgenic plants. Methods for producing dicamba tolerant plants, methods for controlling weed growth, and methods for producing food, feed, and other products are also provided, as well as seed that confers tolerance to dicamba when it is applied pre- or post-emergence.

Abstract: Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include a novel class of EPSPS enzymes, designated Class III, and polynucleotides encoding such enzymes, vectors comprising those polynucleotides, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the Class III domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.

Abstract: Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include a novel class of EPSPS enzymes, designated Class III, and polynucleotides encoding such enzymes, vectors comprising those polynucleotides, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the Class III domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.

Abstract: According to the invention, there is provided a novel soybean variety designated XB005B06. This invention thus relates to the seeds of soybean variety XB005B06, to the plants of soybean XB005B06 to plant parts of soybean variety XB005B06 and to methods for producing a soybean plant produced by crossing plants of the soybean variety XB005B06 with another soybean plant, using XB005B06 as either the male or the female parent.

Abstract: According to the invention, there is provided a novel soybean variety designated XB16S06. This invention thus relates to the seeds of soybean variety XB16S06, to the plants of soybean XB16S06 to plant parts of soybean variety XB16S06 and to methods for producing a soybean plant produced by crossing plants of the soybean variety XB16S06 with another soybean plant, using XB16S06 as either the male or the female parent.

Abstract: According to the invention, there is provided a novel soybean variety designated XB33N06. This invention thus relates to the seeds of soybean variety XB33N06, to the plants of soybean XB33N06 to plant parts of soybean variety XB33N06 and to methods for producing a soybean plant produced by crossing plants of the soybean variety XB33N06 with another soybean plant, using XB33N06 as either the male or the female parent.

Abstract: According to the invention, there is provided a novel soybean variety designated 91M61. This invention thus relates to the seeds of soybean variety 91M61, to the plants of soybean 91M61 to plant parts of soybean variety 91M61 and to methods for producing a soybean plant produced by crossing plants of the soybean variety 91M61 with another soybean plant, using 91M61 as either the male or the female parent.

Abstract: The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5? regulatory sequences for modulating the expression of operably linked genes in plants.

Abstract: The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5? regulatory sequences for modulating the expression of operably linked genes in plants.

Abstract: An improved process for the purification of antibodies from human plasma or other sources is disclosed. The process involves suspension of the antibodies at pH 3.8 to 4.5 followed by addition of caprylic acid and a pH shift to pH 5.0 to 5.2. A precipitate of contaminating proteins, lipids and caprylate forms and is removed, while the majority of the antibodies remain in solution. Sodium caprylate is again added to a final concentration of not less than about 15 mM. This solution is incubated for 1 hour at 25° C. to effect viral inactivation. A precipitate (mainly caprylate) is removed and the clear solution is diluted with purified water to reduce ionic strength. Anion exchange chromatography using two different resins is utilized to obtain an exceptionally pure IgG with subclass distribution similar to the starting distribution. The method maximizes yield and produces a gamma globulin with greater than 99% purity. The resin columns used to obtain a high yield of IgG retain IgM and IgA.

Abstract: The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5? regulatory sequences for modulating the expression of operably linked genes in plants.

Abstract: The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5? regulatory sequences for modulating the expression of operably linked genes in plants.

Abstract: This invention provides purified telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of matrices that bind molecules bearing negative charges, matrices that bind molecules bearing positive charges, intermediate-selectivity matrices, methods that separate molecules based on their size, shape, or buoyant density, and by affinity purification.

Abstract: The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5′ regulatory sequences for modulating the expression of operably linked genes in plants.

Abstract: A method for modifying structural gene sequences to enhance the expression of the protein product is disclosed. Also disclosed are novel structural genes which encode insecticidal proteins of B.t.k. HD-1, B.t.k. HD-73, B.t. tenebrionis, B.t. entomocidus, 2 protein of B.t.k. HD-1, and the coat protein of potato leaf roll virus.

Abstract: Provided is a method for producing transgenic monocotyledonous plants, plant cells, plant parts, seeds, and reproduction material with modified 5-aminolevulinic acid biosynthesis. This is achieved by stably integrating one or several nucleic acid molecules coding for a protein with a 5-aminolevulinic acid synthase function (ALAS) isolated from the alpha group of purple bacteria, an active fragment thereof or an antisense or complementary sequence thereof, into tie plant genome in stable form. This method can also be used to control undesired vegetation Also provided is a method for producing transgenic plants or plant cells whose glutamate-1-semialdehyde transferase (GSAAT) expression is suppressed or inhibited by stable integration of at least one nucleic acid molecule encoding an ALAS isolated from the alpha group of purple bacteria into the plant plastome by plastid transformation.

Abstract: The current invention provides the maize A3 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the A3 promoter directly by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I362697. This invention thus relates to the plants, seeds and tissue cultures of the variety I362697, and to methods for producing a corn plant produced by crossing a corn plant of variety I362697 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I362697 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I362697, and also to the SSR and isozyme typing profiles of corn variety I362697.

Abstract: The present invention relates to a novel plant expression construct. More specifically the present invention provides a DNA construct comprising 5′ regulatory sequences for modulating the expression of operably linked genes in plants.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I465837. This invention thus relates to the plants, seeds and tissue cultures of the variety I465837, and to methods for producing a corn plant produced by crossing a corn plant of variety I465837 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I465837 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I465837, and also to the SSR and isozyme typing profiles of corn variety I465837.

Abstract: An inbred corn line, designated TDC1, is disclosed. The invention relates to the seeds of inbred corn line TDC1, to the plants of inbred corn line TDCI and to methods for producing a corn plant produced by crossing the inbred line TDC1 with itself or another corn line. The invention further relates to hybrid corn seeds and plants produced by crossing the inbred line TDC1 with another corn line.

Abstract: The invention relates in general to plants, plant cells, methods of making, and methods of using plants and plant cells transformed to contain a DNA sequence encoding an AMPA-N-acetyltransferase, and to plants and plant cells exhibiting resistance to AMPA in an amount which inhibits the growth of a plant or plant cell lacking a sequence encoding an AMPA-N-acetyltransferase.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I014738. This invention thus relates to the plants, seeds and tissue cultures of the variety I014738, and to methods for producing a corn plant produced by crossing a corn plant of variety I014738 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I014738 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I014738, and also to the SSR and isozyme typing profiles of corn variety I014738.

Abstract: The current invention provides regulatory regions from the rice actin 2 gene. In particular, the current invention provides the rice actin 2 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the rice actin 2 intron and/or promoter directly by genetic transformation, as well as by plant breeding methods. The actin 2 sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: The present invention provides methods for preparing herbicide tolerant corn plants. Also provided are herbicide tolerant corn plants, as well as seeds and progeny derived from these plants.

Abstract: The instant invention is a novel buffer which enables simultaneous cryoprotection and transfection of mammalian cells. It enables the user to make cell stocks which can be kept long term. Use of these cell stocks circumvents the need to culture cells each time a transfection is undertaken. It alleviates the need for continuous cell culture and repeated transfections due to transfection variability.

Abstract: The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides and a method of selecting transformed plants are also provided.

Abstract: The current invention provides the maize A3 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the A3 promoter directly by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: Methods for classifying plants by remote sensing and image analysis technology are presented. These methods are useful for evaluating plants and for selecting plants for a plant breeding program which has as its goal to selectively alter phenotype. The methods combine the newer techniques of remote sensing technology to obtain indirect correlates of the traits of interest, with classical pedigree breeding strategies. Thermal and infrared reflectance measures of plant canopies are examples of energy values measured by remote sensing, used to indirectly predict the selected traits.

Abstract: An improved process for the purification of antibodies from human plasma or other sources is disclosed. The process involves suspension of the antibodies at pH 3.8 to 4.5 followed by addition of caprylic acid and a pH shift to pH 5.0 to 5.2. A precipitate of contaminating proteins, lipids and caprylate forms and is removed, while the majority of the antibodies remain in solution. Sodium caprylate is again added to a final concentration of not less than about 15 mM. This solution is incubated for 1 hour at 25° C. to affect viral inactivation. A precipitate (mainly caprylate) is removed and the clear solution is diluted with purified water to reduce ionic strength. Anion exchange chromatography using two different resins is utilized to obtain an exceptionally pure IgG with subclass distribution similar to the starting distribution. The method maximizes yield and produces a gamma globulin with greater than 99% purity. The resin columns used to obtain a high yield of IgG retain IgM and IgA.