Process Involving Micro-organisms Of Different Genera In The Same Process, Simultaneously Patents (Class 435/42)
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Patent number: 6599733Abstract: A biodegradation process for the organophosphonate product of Sarin (O-isopropyl methylphosphonofluoridate) hydrolysis, i.e., isopropylmethylphosphonate (IMPA). This process provides a feasible biodegradation demilitarization alternative to Sarin incineration. Public opposition of nerve agent incineration is widespread, and alternative methods are sought to help the U.S. Army meet the 2007 demilitarization deadline imposed by the Chemical Weapons Convention. This process uses a two-step approach to IMPA biodegradation. In the first step, a concentrated IMPA solution is used as the sole nutritional carbon and phosphorus source for microbial cultures. The second step involves diluting the culture and adding an inexpensive carbon source to encourage bacterial phosphate assimilation. The biodegradation typically involves a consortium of microorganisms comprising Methylobacterium radiotolerans GB21, Agrobacterium tumefaciens GB2GA, Klebsiella oxytoca GB2CS, GB272, Aureobacterium sp.Type: GrantFiled: April 27, 2001Date of Patent: July 29, 2003Assignee: The United States of America as represented by the Secretary of the ArmyInventors: Ilona J. Fry, Joseph J. DeFrank, James P. Earley
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Patent number: 6562585Abstract: A method and mixture for denitrifying aerobic bacterial compositions and for aerobic methods for biological treatment of aqueous systems polluted by nitrogen waste products. A mixture of and limited to bacillus bacteria are added to the treatment subject. Optionally enzymes can be added to the mixture. Optionally a particulate carbon ingredient can be placed into the treatment subject. Optionally a living tissue ingredient can be used.Type: GrantFiled: February 14, 2000Date of Patent: May 13, 2003Inventor: William N. Hiatt
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Patent number: 6562586Abstract: An in vitro model system for viral infection is comprised of a tissue block from adult tonsil or lymph node supported on a matrix which is flexible and porous and wherin the supported tissue block is cultured in a medium whose surface is congruent with the tissue block/matrix interface. The histoculture system can be used to screen for antiviral drugs and to monitor the course of viral diseases.Type: GrantFiled: June 12, 2000Date of Patent: May 13, 2003Assignee: AntiCancer, Inc.Inventors: Robert M. Hoffman, Leonid B. Margolis, Joshua Zimmerberg, Svetlana Glushakova
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Patent number: 6541027Abstract: The invention concerns lactic acid bacteria with anxiolytic properties and not inducing sedative effect. Said lactic acid bacteria are useful in particular for preparing foods or medicines.Type: GrantFiled: February 11, 2002Date of Patent: April 1, 2003Assignee: Compagnie Gervais DanoneInventors: Jean-Michel Antoine, Chantal Cayuela, Marie-Christine Degivry, Christian Latge, Eric Postaire
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Patent number: 6531293Abstract: An immobilized microbial consortium is formulated which comprises of a synergistic mixture of isolated bacteria namely, Aeromonas hydrophila, Pseudomonas aeruginosa, Yersinia enterocolitica, Serratia liquefaciens, Pseudomonas fluorescens, Enterobacter cloaca, Klebsiella oxytoca, Citrobacter amalonaticus and Enterobacter sakazaki. The formulated microbial consortium is immobilized on charged nylon membrane. The said immobilized microbial consortium is attached to dissolved oxygen probe for the preparation of electrode assembly. The prepared electrode assembly is used for rapid and reliable BOD estimation. The prepared electrode assembly is used for monitoring of BOD load of synthetic samples such as Glucose-Glutamic acid (GGA) used as a reference standard in BOD analysis and industrial effluents; covering a range from low to high biodegradable organic matter.Type: GrantFiled: March 27, 2000Date of Patent: March 11, 2003Assignee: Council of Scientific and Industrial ResearchInventors: Rita Kumar, Alka Sharma, Shikha Rastogi, Santosh Dayaram Makhijani, A. Manoharan
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Patent number: 6528305Abstract: A simple and efficient method of producing mammalian reovirus is developed using HEK 293 cells. The method provides for fast production of reovirus in high yield. Furthermore, this method provides for a simpler purification procedure of the produced reovirus.Type: GrantFiled: August 2, 2001Date of Patent: March 4, 2003Assignee: Oncolytics Biotech, Inc.Inventors: Bradley G. Thompson, Matthew C. Coffey
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Patent number: 6524842Abstract: A method for degrading an undesirable ether-based environmental contaminant by contacting the ether with a hydrogen-oxidizing microorganism to convert the ether to innocuous compounds which are environmentally acceptable, including treating the ether-based contaminants in situ or removing them from the contaminated site for treatment in a bioreactor, examples of the ether-based contaminants being tertiary butyl ethers of the type utilized as gasoline oxygenates, for example, methyl tert-butyl ether, ethyl tert-butyl ether, and methyl tert-amyl ether, and also ether solvents, for example, tetrahydrofuran.Type: GrantFiled: June 30, 2000Date of Patent: February 25, 2003Assignee: Envirogen, Inc.Inventors: Simon Vainberg, Robert J. Steffan
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Patent number: 6511820Abstract: The present invention relates to novel bacterial strains useful for the production of 2-keto-L-gulonic acid. The present invention further relates to the use of these strains for the production of 2-keto-L-gulonic acid by fermentative conversion of L-sorbose. The present invention further relates to the use of these novel bacterial strains for the production of pyrroloquinoline quinone and a nontoxic lipopolysaccharide. Also described is the strains of the present invention transformed by a vector.Type: GrantFiled: November 28, 2000Date of Patent: January 28, 2003Assignee: Archer-Daniels-Midland CompanyInventor: Steven F. Stoddard
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Patent number: 6511821Abstract: A process for the preparation of a growth media for mass multiplication of bio-control fungi, comprising a chopped, shade-dried distillation waste in a plastic bag that is plugged with cotton followed by autoclaving and an inoculation of a strain of bio-control fungi such as Trichoderma harzianum or Gliocladium virens, and incubating the bags at room temperature for 14-30 days, and then shade drying and grinding the product to obtain a fine powder.Type: GrantFiled: March 19, 2001Date of Patent: January 28, 2003Assignee: Council of Scientific and Industrial ResearchInventors: Harikesh Bahadur Singh, Alok Kalra, Nirmal Kumar Patra, Sushil Kumar, Rakesh Pandey, Suman Preet Singh Khanuja, Ajit Kumar Shasany
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Patent number: 6511822Abstract: An immobilized microbial consortium is formulated which comprises of a synergistic mixture of isolated bacteria namely, Aeromonas hydrophila, Pseudomonas aeruginosa, Yersinia enterocolitica, Serratia liquefaciens, Pseudomonas fluoresces, Enterobacter cloaca, Klebsiella oxytoca, Citrobacter amalonaticus and Enterobacter sakazaki. The formulated microbial consortium is immobilized on charged nylon membrane. The said immobilized microbial consortium is attached to dissolved oxygen probe for the preparation of electrode assembly. The prepared electrode assembly is used for rapid and reliable BOD estimation. The prepared electrode assembly is used for monitoring of BOD load of synthetic samples such as Glucose-Glutamic acid (GGA) used as a reference standard in BOD analysis and industrial effluents; covering a range from low to high biodegradable organic matter.Type: GrantFiled: October 18, 2001Date of Patent: January 28, 2003Assignee: Council of Scientific and Industrial ResearchInventors: Rita Kumar, Alka Sharma, Shikha Rastogi, Santosh Dayaram Makhijani, A. Manoharan
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Patent number: 6506583Abstract: The present invention relates to novel bacterial strains useful for the production of 2-keto-L-gulonic acid. The present invention further relates to the use of these strains for the production of 2-keto-L-gulonic acid by fermentative conversion of L-sorbose. The present invention further relates to the use of these novel bacterial strains for the production of pyrroloquinoline quinone and a nontoxic lipopolysaccharide. Also described is the strains of the present invention transformed by a vector.Type: GrantFiled: November 28, 2000Date of Patent: January 14, 2003Assignee: Archer-Daniels-Midland CompanyInventor: Steven F. Stoddard
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Patent number: 6503739Abstract: Provided is an industrially advantageous process for producing S,S-2-hydroxypropylenediamine-N,N′-disuccinic acid which does not use expensive optically active substances. S,S-2-hydroxypropylenediamine-N,N′-disuccinic acid is stereospecifically produced from fumaric acid and 2-hydroxypropylenediamine by the action of ethylenediamine disuccinic acid ethylenediamine lyase (EDDS-ase) or from maleic acid and 2-hydroxypropylenediamine by combining the action of EDDS-ase with the action of maleic acid isomerase.Type: GrantFiled: October 24, 2001Date of Patent: January 7, 2003Assignee: Mitsubishi Rayon Co., Ltd.Inventor: Makoto Kaneko
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Patent number: 6465207Abstract: An automated method for the silver metal staining of a biological specimen includes treating the biological specimen with a solution including non-gelling gelatin, a solution including a silver salt and a solution including a reducing agent. Non-gelling gelatin is soluble in cold water and the non-gelling gelatin solution can be formed and dispensed onto the biological specimen from liquid dispensers at room temperature. The method can be used in an automated process to detect spirochetes and other bacteria in tissue sections.Type: GrantFiled: November 3, 2000Date of Patent: October 15, 2002Assignee: CytoLogix CorporationInventor: Adrian Elmer Leek
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Patent number: 6461854Abstract: Screening procedures are disclosed for identifying compounds useful for inhibiting infection or pathogenicity. Methods are also disclosed for identifying pathogenic virulence factors.Type: GrantFiled: March 28, 1995Date of Patent: October 8, 2002Assignee: The General Hospital CorporationInventors: Frederick M. Ausubel, Laurence G. Rahme, Man-Wah Tan, Gary B. Ruvkun
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Patent number: 6451567Abstract: A process is provided for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids. Further, a process for producing lipids includes a fermentation by growing euryhaline microorganisms which are capable of producing 1.Type: GrantFiled: December 14, 1999Date of Patent: September 17, 2002Assignee: Omegatech, Inc.Inventor: William R. Barclay
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Patent number: 6444437Abstract: Rural biomass and other cellulosic materials are converted to a protein-enriched animal feed supplement or to single-cell protein by a series of bio-reactions. A first stage bio-reaction is a solid substrate bio-reaction. Enzymes, such as cellulase, produced by the first-stage bio-reaction are added to a second-stage bio-reaction. Raw second-stage bio-reaction feedstock is pretreated to hydrolyze hemicellulose and/or to partially digest starch in the feedstock. In the second-stage bio-reaction, the feedstock is substantially digested and single-cell protein is harvested in an aerobic bio-reaction, while ethanol is produced in an anaerobic reaction. Products of the invention can serve as feed supplements to enhance protein content of animal feed.Type: GrantFiled: July 14, 1999Date of Patent: September 3, 2002Assignee: Colorado State University Research FoundationInventors: Robert A. Sporleder, James C. Linden, Herbert A. Schroeder, Donald Johnson, Linda L. Henk, Robert P. Tengerdy, George Szakács
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Publication number: 20020119123Abstract: The present invention discloses high density antagonistic microbe base materials and a method of producing the same, more particularly, a method for—preparing high density antagonistic microbe base material comprising the steps of: inoculating a mixture of native microbes as main components to organic medium to obtain crude bacteria by subculture; and further inoculating the obtained crude bacteria in a culture medium consisting or crushed soil from volcanic rocks mineral, combined nutrients and water, culturing and then being subjected to air ventilation under agitating the cultured material along the rise in temperature.Type: ApplicationFiled: October 2, 2001Publication date: August 29, 2002Inventor: Shinya Tsuru
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Immobilized microbial consortium for the treatment of phenolic waste-water from petroleum refineries
Patent number: 6406882Abstract: An immobilized microbial consortium is formulated which comprises of a synergistic mixture of all the bacterial strains of Aeromonas hydrophila, Pseudomonas fluorescens, Pseudomonas aeruginosa, Bacillus circulans, Yersinia enterocolitica, Enterobacter cloaca and Bacillus brevis. The formulated microbial consortium is immobilized on a non-biodegradable and economically cheaper support. The said immobilized microbial consortium is used for the biodegradation of synthetic phenol as well as phenol present in petroleum refinery effluent. The results of biodegradation obtained with the microbial consortium immobilized on coconut fiber are compared with those obtained with microbial consortium immobilized on well known support. The coconut fiber used for immobilization proved to be a better support than a well known support such as calcium alginate.Type: GrantFiled: March 27, 2000Date of Patent: June 18, 2002Assignee: Council for Scientific and Industrial ResearchInventors: Rita Kumar, Alka Sharma, Archana Kumar -
Patent number: 6403084Abstract: Methods and compositions for treatment of animal feed or silage by treatment with a mixed culture of heterofermentive lactic acid bacteria and homofermentive lactic acid bacteria of the proper ratio. Bacterial strains for such treatment are also provided.Type: GrantFiled: November 3, 2000Date of Patent: June 11, 2002Assignee: Pioneer Hi-Bred International, Inc.Inventors: Russell Kuo-fu Chan, Scott Dennis, Elizabeth K. Harman, William Rutherford, Brenda Smiley
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Patent number: 6387654Abstract: The present invention relates to novel bacterial strains belonging to the genera Gluconobacter, Ketogulonogenium and Bacillus useful for the production of 2-keto-L-gulonic acid. The present invention further relates to the use of these strains for the production of 2-keto-L-gulonic acid in mixed culture by fermentative conversion of D-sorbitol. The present invention further relates to the strains of the present invention transformed by a vector.Type: GrantFiled: May 4, 2000Date of Patent: May 14, 2002Assignee: Archer-Daniels-Midland CompanyInventors: Hungming J. Liaw, Robert L. Kowzic, Jr., John M. Eddington, Yuequin Yang
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Patent number: 6379956Abstract: The populating method for populating a substrate with living cells provides a population with at least one populating phase and at least one perfusion phase. During the populating phase the substrate can be held in contact with the cell suspension by continuous rotation in various spatial arrangements. The populating device of the invention (10) comprises a rollable container (10a) and a removable insert in it with a means (12) for inserting the substrate being populated, such as a plastic or collagen matrix. The insert is designed so that in interaction with the inserted substrate, two liquid-tight chambers are formed within the container on opposite sides of the inserted substrate, so as to allow separate populating of both sides of the substrate. The container have liquid inlets and outlets (16) and may also have gas inlets and outlets. The rollable populating reactor (10) is inserted into a roller cabinet for certain phases of the populating.Type: GrantFiled: February 21, 2001Date of Patent: April 30, 2002Inventor: Augustinus Bader
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Patent number: 6376213Abstract: A polyester containing aromatic moieties is contacted with microorganisms having the activity of decomposing the polyester to decompose or reduce it. Preferably, either or both of Trichosporon FERM BP-6445 or Arthrobacter FERM BP-6444 was contacted with the polyester to decompose or reduce it. A fiber made of the polyester or a cloth made of such fiber may be reduced by contacting it with the microorganisms having the activity of decomposing the polyester.Type: GrantFiled: July 14, 1999Date of Patent: April 23, 2002Assignee: Kyoto Institute of TechnologyInventors: Kohei Oda, Yoshiharu Kimura
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Patent number: 6368819Abstract: A modified water-immiscible solvent useful in the extraction of acetic acid from aqueous streams is a substantially pure mixture of isomers of highly branched di-alkyl amines. This solvent is substantially devoid of mono-alkyl amines and alcohols. Solvent mixtures formed of such a modified solvent with a desired cosolvent, preferably a low boiling hydrocarbon which forms an azeotrope with water are useful in the extraction of acetic acid from aqueous gaseous streams. An anaerobic microbial fermentation process for the production of acetic acid employs such solvents, under conditions which limit amide formation by the solvent and thus increase the efficiency of acetic acid recovery. Methods for the direct extraction of acetic acid and the extractive fermentation of acetic acid also employ the modified solvents and increase efficiency of acetic acid production.Type: GrantFiled: March 7, 2001Date of Patent: April 9, 2002Assignees: Bioengineering Resources, Inc., Celanese International CorporationInventors: James L. Gaddy, Edgar C. Clausen, Ching-Whan Ko, Leslie E. Wade, Carl V. Wikstrom
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Patent number: 6368623Abstract: The invention relates to a pulverulent corn-steep without a drying substrate, said corn-steep containing lactic acid. It also relates to a method of obtaining this pulverulent corn-steep as well as to its use in the fermentation industry.Type: GrantFiled: April 4, 2000Date of Patent: April 9, 2002Assignee: Roquette FreresInventors: Thomas Erpicum, Marie-Hélène Saniez
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Patent number: 6350605Abstract: This invention relates to a novel microorganism capable of oxidizing manganese such as the genus Cedecea bacterium GSJ/MITA24A/ASHO-RO/1, the genus Aeromonas bacterium GSJ/MITA24B/ASHO-RO/2, or the genus Shewanella bacterium GSJ/MITA24C/ASHO-RO/3; to a method for removing manganese from water containing manganese, which comprises contacting the water containing manganese with a microbial symbiont of algae and one or more microorganisms capable of oxidizing manganese to oxidize and precipitate the manganese, thereby removing the manganese from the water; and to a method of recycling the recovered manganese.Type: GrantFiled: December 22, 1999Date of Patent: February 26, 2002Inventors: Naoki Mita, Yoshishige Kato, Akihiko Maruyama, Takanori Higashihara, Yutaka Kanai, Akira Usui, Hiroyuki Miura, Takashi Ito, Hidetoshi Tashiro
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Patent number: 6348326Abstract: L-Ribose is produced by the steps of producing ribitol from a saccharide raw material by using a microorganism having an ability to produce ribitol from a saccharide raw material, producing L-ribulose from the ribitol by using a microorganism having an ability to produce L-ribulose from ribitol, and producing L-ribose from the L-ribulose by using a microorganism having an ability to produce L-ribose from L-ribulose.Type: GrantFiled: November 27, 2000Date of Patent: February 19, 2002Assignee: Mitsubishi Chemical CorporationInventors: Tomoko Kawaguchi, Mari Hara, Makoto Ueda
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Patent number: 6261798Abstract: The present invention relates to a method of producing [S,S]-ethylenediamine-N,N′-disuccinate wherein a microorganism having malate isomerase activity or matter processed therefrom and a microorganism having ethylenediamine-N,N′-disuccinate ethylenediamine lyase activity or matter processed therefrom are allowed to act on a substrate solution containing maleic acid, maleic anhydride, or a maleic acid salt, and ethylenediamine, in the presence of at least one metal ion selected from the group consisting of alkaline earth metals, iron, zinc, copper, nickel, aluminum, titanium and manganese. The present invention enables to accumulate [S,S]-ethylenediamine-N,N′-disuccinate in a higher yield and at a high concentration within a reaction system using maleic acid as a raw material.Type: GrantFiled: June 22, 2000Date of Patent: July 17, 2001Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Osamu Kato, Makoto Kaneko, Takakazu Endo
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Publication number: 20010007763Abstract: This invention relates to a process for producing a complex carbohydrate, which comprises: selecting, as enzyme sources, a culture broth of a microorganism capable of producing a sugar nucleotide from a nucleotide precursor and a sugar, or a treated product of the culture broth, and a culture broth of a microorganism or animal cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth; carrying out an enzyme reaction in an aqueous medium containing the enzyme sources, the nucleotide precursor, the sugar and the complex carbohydrate precursor to form and accumulate the complex carbohydrate in the aqueous medium; and recovering the complex carbohydrate from the aqueous medium, and a process for producing a sugar nucleotide, which comprises selecting, as an enzyme source, a culture broth of a microorganism capable of producing a sugar nucleotide from a nucleotide precursor and a sugar, or a treated product of the culturType: ApplicationFiled: May 13, 1998Publication date: July 12, 2001Inventors: SATOSHI KOIZUMI, HISAJI KAWANO, KUNIKI KINO, AKIO OZAKI
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Patent number: 6238897Abstract: A process for the continuous production of 2-keto-L-gulonic acid or a salt thereof from D-sorbitol by fermentation with microorganisms in a nutrient medium containing D-sorbitol that is incubated in a first fermentation vessel with a microorganism capable of converting D-sorbitol to L-sorbose, whereafter the resulting fermentation broth containing L-sorbose is transferred to a second fermentation vessel where it is incubated with a microorganism capable of converting L-sorbose to 2-keto-L-gulonic acid. In a particularly preferred embodiment of this process, the fermentation broth from the first fermentation vessel is sterilized before being transferred to the second fermentation vessel. 2-keto-L-gulonic acid is a valuable intermediate for the production of vitamin C.Type: GrantFiled: July 13, 1999Date of Patent: May 29, 2001Assignee: Roche Vitamins Inc.Inventor: Markus Rückel
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Patent number: 6238906Abstract: A bacterial culture capable of degrading ethers, especially branched alkylethers including MTBE, under aerobic conditions has been prepared.Type: GrantFiled: April 14, 1999Date of Patent: May 29, 2001Assignee: Shell Oil CompanyInventor: Joseph Patrick Salanitro
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Patent number: 6221847Abstract: A method and composition for preventing and treating infection from clinically relevant bacterial isolates is disclosed. The composition contains an anti-bacterial agent that inhibits the growth of the bacteria, including members of both gram negative and gram positive isolates. In particular, the anti-bacterial agent comprises a bacteriocin produced by Propionibacterium. For instance, in one embodiment, the bacteriocin is produced by the B1264 strain of Propionibacterium jensenii.Type: GrantFiled: May 27, 1999Date of Patent: April 24, 2001Assignee: Clemson UniversityInventors: Susan F. Barefoot, Priya Ratnam
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Patent number: 6165757Abstract: A fermentation process is disclosed for producing an HMG-CoA reductase inhibitor such as lovastatin or compactin. In particular, it relates to a process wherein at least part of the assimilable nitrogen source and, optionally, the carbon source is provided continuously or intermittently to the culture.Type: GrantFiled: September 15, 1999Date of Patent: December 26, 2000Assignee: DSM N.V.Inventors: Adriaantje Ykema, Jennifer May Lindsay
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Patent number: 6124112Abstract: The invention relates to a method of production for a fermentation product, in which ripe compost is subjected to treatment with worms, bacteria and fungi, after which the fermentation product is dug off, and, if necessary, ground and sifted. After digging off the product, it can be pre-dehydrated at a temperature below 50 .degree. C., if one so wishes, then --if necessary--it can be ground and sifted, and be subjected to desiccation, during which the temperature of the fermentation product does not rise above 30.degree. C. during the desiccation process. Ripe compost, consisting of vegetable, fruit and garden waste, is especially suitable as basic material. If one so wishes, the fermentation product can be pelleted before desiccation. The pellets can, for example, be used as floor cover in animal pens or as cat's box litter. The non-dried product is especially suitable as a supplement for garden and pot-plant soil.Type: GrantFiled: April 30, 1997Date of Patent: September 26, 2000Inventor: Jan Molenaar
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Patent number: 6121033Abstract: Degradable polyesters useful in packaging, packing, agricultural, biomedical, and other applications are made by reacting amine-protected glutamic acid with diols or epoxy compounds. The polyesters include a thermoplastic main chain aliphatic polyester, a thermoset heterochain polyester and a thermoset heterochain aromatic polyester. Each of these polyesters can be hydrolyzed into monomers using a biological catalyst such as the enzyme lipase. The thermoplastic main chain aliphatic polyester and the thermoset heterochain polyester can be degraded to respiratory gases and biomass with a mixed culture of Rhizopus chinesis, Rhizopus delemar, Penecillium pinophilum, Aspergillus niger and Pseudomonas aeruginosa microorganisms. This mixed culture of microorganisms can also be used to degrade other polyesters containing hydrolyzable backbone polyesters.Type: GrantFiled: July 21, 1999Date of Patent: September 19, 2000Assignee: The Board of Regents of the University of NebraskaInventors: Raef M. Tadros, Delmar C. Timm
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Patent number: 6110875Abstract: Xanthan molecules are degraded using an xanthanase enzyme complex that is stable at temperatures above 250.degree. F., such as those temperatures found in some wellbores and process streams. The xanthanase enzyme complex is produced by a novel soil bacterium. The xanthanase enzyme complex may be used to remove xanthan based formation damage, such as drilling filter-cakes and filtrates, or to remove xanthan based filter-cakes and/or residues present in processing equipment. The xanthanase enzyme complex may also be used to reduce the viscosity of xanthan-containing fluids, such as hydraulic fracturing fluids, blocking gels, drilling muds, and process fluids. The xanthanase enzyme complex may also be used in conjunction with other well or process treatments, such as stimulations and cementing operations, to improve the effectiveness of these treatments.Type: GrantFiled: March 7, 1997Date of Patent: August 29, 2000Assignee: BJ Services CompanyInventors: Robert M. Tjon-Joe-Pin, Michelle Alana Carr, Bing Yang
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Patent number: 6043022Abstract: An apparatus and method for the generation and use of ferric ions produced by acidophilic, chemoautotrophic bacteria.Type: GrantFiled: August 25, 1998Date of Patent: March 28, 2000Assignee: Board of Control of Michigan Technological UniversityInventors: Donald R. Lueking, Carl C. Nesbitt
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Patent number: 6040154Abstract: A process for treating aqueous effluents that contain ethyl-tert-butyl ether (ETBE) to reduce the ETBE concentration is described, characterized in that in the presence of effluents, at least one bacterium that is selected from the group that is formed by Gordona terrae CIP I-1889 and Rhodococcus equi CIP I-2053 and in the presence of at least one bacterium that is selected from the group that is formed by Pseudomonas cepacia CIP 1-2052, Arthrobacter globiformis ATCC 53596, Bacillus coagulans ATCC 53595, Pseudomonas stutzerii ATCC 53602 and Mycobacterium vaccae JOB5 are grown to degrade essentially all of the ETBE. The concentration of ETBE in the effluent is equal to at most 1500 mg/L. Application for the water treatment industry.Type: GrantFiled: July 23, 1998Date of Patent: March 21, 2000Assignee: Institut Francais du PetroleInventors: Francoise Fayolle, Francoise Le Roux, Jean-Paul Vandecasteele
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Patent number: 6025152Abstract: A method and mixture for denitrifying aerobic bacterial compositions and for aerobic methods for biological treatment of aqueous systems polluted by nitrogen waste products. A mixture of and limited to bacillus bacteria are added to the treatment subject. Optionally enzymes can be added to the mixture. Optionally a particulate carbon ingredient can be placed into the treatment subject. Optionally a living tissue ingredient can be used.Type: GrantFiled: June 3, 1997Date of Patent: February 15, 2000Inventor: William N. Hiatt
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Patent number: 6007981Abstract: The cDNA that encodes a glycoprotein receptor from the tobacco hornworm which binds a Bacillus thuringiensis toxin has been obtained and sequenced. The availability of this cDNA permits the retrieval of DNAs encoding homologous receptors in other insects and organisms as well as the design of assays for the cytotoxicity and binding affinity of potential pesticides and the development of methods to manipulate natural and/or introduced homologous receptors and, thus, to destroy target cells, tissues and/or organisms.Type: GrantFiled: December 10, 1997Date of Patent: December 28, 1999Assignee: University of WyomingInventor: Lee A. Bulla
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Patent number: 5986095Abstract: A process is described for preparing a compound of the formula or the S-enantiomer thereof,wherein:R is alkyl, aryl, cycloalkyl, aralkyl, or cycloalkylalkyl,R.sup.1 is halogen;R.sup.2 is halogen, alkyl, cycloalkyl, aryl or ##STR1## wherein the process comprises treating the associated racemic alcohol with an acylatingagent ##STR2## (wherein L is a leaving group) and an enzyme or microorganism capable of enantioselective acylation. This process may also be used to isolate the unreacted R- or S-alcohol. The acylated product may be enantioselectively hydrolyzed with a lipase or lipase-supplying microorganism to the S- or R-alcohol. Compounds prepared by this invention are useful antipsychotic agents or useful intermediates therefor.Type: GrantFiled: January 6, 1992Date of Patent: November 16, 1999Assignee: E.R. Squibb & Sons, Inc.Inventors: Ronald L. Hanson, Ramesh N. Patel, Laszlo J. Szarka
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Patent number: 5981473Abstract: A method and composition for preventing and treating acne is disclosed. The composition contains an anti-bacterial agent that inhibits the growth of the cutaneous bacteria that are believed to cause acne. In particular, the anti-bacterial agent comprises a bacteriocin produced by Propionibacterium. For instance, in one embodiment, the bacteriocin is produced by the B1264 strain of Propionibacterium jensenii. The bacteriocins of the present invention can be contained in a topical preparation that is applied to the skin.Type: GrantFiled: September 12, 1997Date of Patent: November 9, 1999Assignee: Clemson UniversityInventors: Susan F. Barefoot, Priya Ratnam
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Patent number: 5955090Abstract: The present invention relates to a hybrid protein comprising the Pseudomonas aeruginosa outer membrane protein I (OprI) which is fused with its amino terminal end to the carboxy-terminal end of a carboxy-terminal portion of the Pseudomonas aeruginosa outer membrane protein F (OprF), as well as to monoclonal or polyclonal antibodies against this hybrid protein. Both, the hybrid protein and the antibodies directed to the hybrid protein confer protection against an infection by Pseudomonas aeruginosa to laboratory animals or man.Type: GrantFiled: December 14, 1995Date of Patent: September 21, 1999Assignee: Chiron Behring GmbH & Co.Inventors: Bernhard Knapp, Klaus-Dieter Hungerer, Michael Broker, Bernd-Ulrich von Specht, Horst Domdey
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Patent number: 5952188Abstract: A reusable immobilized microbial composition is formulated. The formulated microbial composition comprises a synergistic mixture of the bacterial strains of Enterobacter cloaca, Citrobacter amalonaticus, Pseudomonas aeruginosa,Yersinia enterocolitica, Klebsiella oxytoca, Enterobacter sakazaki and Serratia liquefaciens. The formulated microbial composition is immobilized on an appropriate immobilizing agent to form beads. The said beads are tested as microbial seeding material for BOD analysis using Glucose Glutamic Acid (GGA) as a reference standard. The obtained BOD values by the formulated beads are compared with BOD values obtained by sewage as seeding material using synthetic samples as well as industrial effluents. The formulated microbial beads are ready-to-use as well as reusable seeding material in BOD analysis. The said beads can be reused up to five times with same efficacy.Type: GrantFiled: July 10, 1998Date of Patent: September 14, 1999Assignee: Council of Scientific & Industrial ResearchInventors: Rita Kumar, Anil Kumar, Alka Sharma, Sharad Vishwanath Gangal, Santosh Daya Ram Makhijani
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Patent number: 5952020Abstract: A process of conversion of low protein, cellulose containing waste into a fodder or fodder supplement is provided.Type: GrantFiled: February 2, 1999Date of Patent: September 14, 1999Assignee: Bio-Feed Ltd.Inventor: Yuri Lizak
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Patent number: 5902734Abstract: An isolated mixed bacterial culture, preferably BC-1, ATCC No. 202057, which degrades ethers, especially branched alkylethers including MTBE, under aerobic conditions has been prepared.Type: GrantFiled: December 24, 1997Date of Patent: May 11, 1999Assignee: Shell Oil CompanyInventor: Joseph Patrick Salanitro
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Patent number: 5874291Abstract: This invention pertains to substantially purified cultures of a gram-negative, aerobic, filamentous bacterium with cells ranging in length from 20-200 .mu.m, that accumulates intracellular poly-.beta.-hydroxybutyrate in intracellular granules, and that degrades chlorinated aliphatic compounds such as trichloroethylene and dichloroethylene, as well as phenol and other substituted benzenes. The invention includes the representative strain A-1, which has been deposited at the American Type Culture Collection under the accession number 55581. Also included are methods for using the new bacterium for bioremediation of contaminated environmental sites.Type: GrantFiled: April 30, 1996Date of Patent: February 23, 1999Assignee: University of WashingtonInventors: Angela R. Bielefeldt, H. David Stensel, Stuart E. Strand, Russell P. Herwig
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Patent number: 5858763Abstract: The invention is a consortium of thermophilic methanotrophic organisms in culture medium containing said consortium reproduced at temperatures of 50.degree. C. to 80.degree. C., said consortium being comprised primarily of ovoid or rod-shaped organisms. The consortium can be instilled into soil or water to degrade pollutants, especially hydrocarbons and substituted hydrocarbons.Type: GrantFiled: April 25, 1997Date of Patent: January 12, 1999Assignees: The United States of America as represented by the Administrator of the Environmental Protection Agency, University of CincinnatiInventors: Wendy J. Davis-Hoover, Stephen J. Vesper
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Patent number: 5856141Abstract: The present invention relates to a process for making a highly productive fusant of Tolypocladium inflatum, a producing strain of cyclosporin A with immunosuppressive properties wherein the selection of the fusant KD461, designed to produce a large amount of cyclosporin A, was made available by the following steps of: developing amino acid-dependent mutants of Tolypocladium inflatum, wild strain isolated from soil, which mutants are induced by UV radiation; conjugating L-valine-dependent and L-leucine-dependent mutants to promote the demand and utility of L-valine and L-leucine, precursors of cyclosporin A, together with an organic nitrogen-source.Type: GrantFiled: April 18, 1997Date of Patent: January 5, 1999Assignee: Chong Kun Dang Corp.Inventors: Jung Woo Kim, Kwang Moo Lee, Byoung Tack Choi, Jin Man Lee, Nak Kyu Sung, Kyeong Bok Min
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Patent number: 5854032Abstract: Coal is treated aerobically or anaerobically to produce humic acid, volatile fatty acids, lower alcohols, and/or methane using a consortium of bacteria designated Mic-1 or KSARC56. This process can also be used to convert aromatic compounds, such as phenols and derivatives thereof, to methane and carbon dioxide.Type: GrantFiled: September 24, 1996Date of Patent: December 29, 1998Assignee: Arctech, Inc.Inventors: Kailash Chandra Srivastava, Daman S. Walia
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Patent number: 5846760Abstract: The method of the invention involves providing a first receptacle and a second receptacle. The first receptacle contains a sterile aqueous broth and the second receptacle contains an aqueous broth including a carbon source. The method then includes placing into the first receptacle a first support surface having a paraffin wax coating thereon and placing into the second receptacle a second support surface having a hydrophobic material coating thereon. A body specimen, such as sputum, is then introduced into each of the first and second receptacles. The presence of a nonparaffinophilic hydrophobic microorganism in the body specimen is determined by observing (i) a lack of microorganism growth on the paraffin coated material of the first support surface and (ii) a presence of microorganism growth on the hydrophobic material coating of the second support surface.An associated kit is also disclosed.Type: GrantFiled: November 13, 1997Date of Patent: December 8, 1998Assignee: Infectech, Inc.Inventor: Robert-A. Ollar